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gas-forming brain abscess caused by klebsiella pneumoniae.gas forming brain abscess is a rare disease caused by klebsiella pneumoniae occurring in patients with impaired host defense mechanism such as diabetes mellitus or liver cirrhosis. a 59-year-old man with 2-year history of diabetes mellitus and 20-year history of liver cirrhosis presented to the hospital with headache. on the day after admission, severe headache was developed and he deteriorated rapidly. brain ct showed a non-enhanced mass including multiple air density as well as surrounding ede ...200819137083
klebsiella pneumoniae lo10 producing extended-spectrum beta-lactamase shv-12 in saudi arabia.klebsiella pneumoniae lo10 was responsible for an outbreak that occurred in the neonatal unit at security forces hospital, kingdom of saudi arabia. over a period of eight months nine cases of bacteremia resulted in two deaths. resistance to third generation cephalosporins was transferred from strain lo10 to e. coli by both conjugation and transformation. sequence determination of the plasmid gene from two transconjugants and one transformant indicated that resistance was carried by a ca.100-kb p ...200819129068
antimicrobial resistance among uropathogens responsible for community-acquired urinary tract infections in an italian community.this study aimed to establish the pattern of the antimicrobial resistance among the leading uropathogens causing community-acquired utis in an area of the region of apulia, southern italy. twenty-one thousand and two hundred outpatients, 6,893 males and 14,307 females, were enrolled. urinary isolates were identified by conventional methods and the susceptibility to 18 antimicrobials determined. recognized uropathogens escherichia coli, klebsiella pneumoniae and proteus mirabilis were isolated fr ...200819129070
first report of ciprofloxacin resistance among klebsiella pneumoniae harbouring the qnra1 gene and producing shv-5 extended-spectrum beta-lactamase in scotland. 200819129076
occurrence and detection of ampc beta lactamases among clinical isolates of e. coli and k. pneumoniae causing uti.presence of bush class c enzymes in uropathogenic strains of klebsiella pneumoniae & e. coli resistant to extended spectrum cephalosporins is an emerging threat to clinical therapeutics. these resistant strains result in considerable treatment failure and cannot be detected by routine antibiotic sensitivity screening methods. an effort was therefore made to study ampc beta lactamase production in e. coli and klebsiella pneumoniae strains causing uti. a total of 126 e. coli and 49 k. pneumnoniae ...200819127665
laboratory detection of extended-spectrum beta-lactamase by an automated system.this study aims to evaluate the positive predictive value (ppv) and the negative predictive value (npv) of vitek2 in detecting extended-spectrum beta-lactamase (esbl) phenotypes when compared to a manual confirmatory test as gold standard. a sample of escherichia coli, klebsiella spp and proteus mirabilis isolates were collected by 5 laboratories in the emilia-romagna region (italy). vitek2 appears to be an accurate tool to detect esbl phenotypes of e. coli isolates; some concern remains about i ...200819123314
ctx-m type beta-lactamases among fecal escherichia coli and klebsiella pneumoniae isolates in non-hospitalized children and adults.we investigated the occurrence and diversity of extended-spectrum beta-lactamase (esbl) enzymes among antibiotic-resistant escherichia coli and klebsiella pneumoniae isolates obtained from human feces. all esbl-positive isolates were characterized at the molecular level by polymerase chain reaction, sequencing and pulsed-field gel electrophoresis (pfge). eight of 46 antibiotic-resistant e. coli (6 from children and 2 from adults) and 4 of 8 k. pneumoniae (all from adults) isolates were found to ...200819122926
antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in rainforest.the aim of this study was to screen antitumour and antimicrobial activities of endophytic actinomycetes isolated from pharmaceutical plants in rainforest in yunnan province, china.200819120929
protection of immuno-compromised mice from lethal infection of klebsiella pneumonia by raav2-bpi23-fcgamma1 gene transfer.in previous research, chimerical bpi23-fcgamma1 gene which consisted of human bactericidal/permeability increasing protein (bpi) gene of encoding the functional n terminus (amino acid residues 1 to 199) of human bpi and fcgamma1 gene of encoding the fc segment of human immunoglobulin g1 was successfully reconstructed within a recombinant adeno-associated virus serotype 2 (raav2) vector as raav2-bpi23-fcgamma1. here, to evaluate the potentiality of applying gene therapy to gram negative bacterial ...200819118510
pyogenic liver abscess associated with septic pulmonary embolism. 200819114323
xanthogranulomatous pyelonephritis successfully treated with antibiotics only.a 73-year-old woman with leukemia presented with urinary tract infection, splenic abscess, and a renal mass. both urine culture and pus culture of the splenic abscess yielded klebsiella pneumoniae. percutaneous biopsy of the renal mass confirmed the diagnosis of xanthogranulomatous pyelonephritis. because of high risk for surgery, the patient received treatment with antibiotic therapy for 2 months. with antibiotic therapy, not only was the splenic abscess cured but follow-up ultrasonography also ...200819114330
magnetic resonance imaging findings in a red kangaroo (macropus rufus) with otitis.magnetic resonance imaging (mri) was performed on an adult, male red kangaroo (macropus rufus) with a history of nonspecific neurologic signs and acute discharge from the left ear. mri revealed findings consistent with otitis and possible osteomyelitis of the temporal and mastoid bones. to the authors' knowledge, this is the first report of otitis and mri findings in a kangaroo.200819110716
use of the iso 9308-1 procedure for the detection of e. coil in water utilizing two incubation temperatures and two confirmation procedures and comparison with defined substrate technology.disinfected and non-disinfected samples have been used to determine the accuracy of the iso procedure (iso 9308-1) for detection of e. coli in drinking water. samples were analysed using the iso procedure at both 36 and 44 degrees c and using the defined substrate technology method colilert-18/quanti-tray (colilert-18). utilizing the confirmation procedure described in iso 9308-1, large numbers of false positive e. coli results were obtained using the iso primary isolation procedure at 36 degree ...200819108560
update on antimicrobial susceptibility rates among gram-negative and gram-positive organisms in the united states: results from the tigecycline evaluation and surveillance trial (test) 2005 to 2007.the tigecycline evaluation and surveillance trial (ttest) is a global surveillance study initiated in 2004.its goal is to assess the in vitro activity of the glycylcycline, tigecycline, and comparator antimicrobials. objective: the aim of this study was to measure the in vitro activity of a panel of antimicrobial agents against gram-negative and gram-positive organisms collected in the united states in 2005, 2006, and 2007.200819108792
[comparative analysis of the pathogens responsible for hospital acquired and community acquired late onset neonatal septicemia].late onset neonatal septicemia (systemic infection after 72 hours of life) remains a major cause of neonatal morbidity and mortality. early treatment with appropriate antibiotics is critical since infected infants can deteriorate rapidly. the aim of this study was to review the pathogens responsible for late onset neonatal septicemia (lons) and their antimicrobial susceptibilities in order to guide the initial selection of appropriate antibiotics for infants with suspected lons.200819099687
[bacterial etiology of pneumonia in hospitalized children: combined detection with culture and polymerase chain reaction].bacterial cultures from respiratory aspirate or sputum have been the conventional diagnostic method for pneumonia, but the results of culture was often affected by early extensive use of antibiotics, sample collection and delivery. the objective of this study was to explore application of the combined detection of culture and polymerase chain reaction (pcr) assay in hospitalized children with pneumonia.200819099875
dissemination of extended-spectrum beta-lactamase-producing klebsiella pneumoniae and escherichia coli in thai hospitals.fifty clinical isolates of klebsiella pneumoniae and escherichia coli with reduced susceptibility to third-generation cephalosporins, collected from 11 hospitals in thailand, were studied. all isolates were found to produce extended-spectrum beta-lactamase (esbl), as judged by double-disk synergy and combination disk methods. most esbl-producing k. pneumoniae isolates were resistant to ceftazidime (94%) and aztreonam (90%). in contrast, most esbl-producing e. coli isolates were resistant to ceft ...200819089552
[severe laryngotracheal involvement in a case of rhinoscleroma due to klebsiella pneumoniae subspecies ozaenae]. 200819091241
[pathogens and drug resistance of pulmonary infection in aids patients].to study the pathogens and drug resistance profiles of pulmonary infection in patients with aids.200819080136
[risk factors and prognosis in 31 patients with extended-spectrum beta-lactamase producing escherichia coli and klebsiella pneumoniae bloodstream infection].to investigate the risk factors, prognosis and resistance to antibiotics in patients with extended-spectrum b-lactamase (esbls)-producing escherichia coli and klebsiella pneumoniae bloodstream infection.200819080534
epidemiology of bloodstream infection associated with parenteral nutrition.epidemiology: catheter-related bloodstream infections (cr-bsis) occur in 1.3% to 26.2% of patients with central venous catheters used to administer parenteral nutrition (pn). because of their nutritional components, pn solutions can support microbial growth. contamination during preparation and handling is rare in hospitals and home-infusion pharmacies but may be difficult to control in a home setting. the risk of infection is increased in hospitalized patients because of malnutrition-associated ...200819084152
microbiology of gallbladder bile in uncomplicated symptomatic cholelithiasis.few studies have assessed microflora and their antibiotic sensitivity in normal bile and lithogenic bile with different types of gallstones.200819073410
antimicrobial resistance of bacterial agents of the upper respiratory tract of school children in buea, cameroon.the study was aimed at determining bacterial agents of the upper respiratory tract and the susceptibility patterns of isolates to antibiotics. in total, 200 throat swabs were obtained from students attending different boarding schools within the buea municipality and screened to obtain the prevalence of respiratory pathogens and to understand the antibiotic susceptibility patterns of isolates using standard microbiological procedure and the disc-diffusion test. of the 200 samples screened, 112 ( ...200819069618
prevalence of bacteria and intestinal parasites among food-handlers in gondar town, northwest ethiopia.food-handlers with poor personal hygiene working in food-service establishments could be potential sources of infection due to pathogenic organisms. the study was undertaken to determine the prevalence of bacteria and intestinal parasites among 127 food-handlers working in the cafeterias of the university of gondar and the gondar teachers training college, gondar, ethiopia. fingernail contents of both the hands and stool specimens were collected from all the 127 food-handlers. the samples were e ...200819069624
changing trend in antimicrobial resistance of pediatric uropathogens in taiwan.there is growing concern regarding antimicrobial resistance worldwide, particularly of escherichia coli, and the first choice of an antimicrobial agent for empiric treatment of pediatric urinary tract infection (uti) is not well established.200819067894
[detection of ampc beta-lactamases in gram-negative bacteria isolated from urine].ampc beta-lactamases, enzymes produced by a range of gram-negative bacteria and able to hydrolyse penicillins, monobactams, cephalosporins and cephamycins, are not inhibited by beta-lactamase inhibitors. they are spread via plasmids and pose a huge epidemiological and diagnostic problem. the production of ampc b-lactamases was tested in various gram-negative bacteria. of strains isolated from urine of patients diagnosed with urinary tract infection, we selected those that were resistant to third ...200819069027
study on the bacterial midgut microbiota associated to different brazilian populations of lutzomyia longipalpis (lutz & neiva) (diptera: psychodidae).the bacterial community associated with the midgut of three brazilian lutzomyia longipalpis (lutz & neiva) populations, two from endemic areas for visceral leishmaniasis (jacobina, bahia state and são luís, maranhão state) and one from a non-endemic area (lapinha cave, minas gerais state), was identified. five groups, 35 females each, from each population were separated; a total of 175 females per collecting area were analyzed. the species identification was based on molecular and traditional ba ...200819061048
ablation of sp-a has a negative impact on the susceptibility of mice to klebsiella pneumoniae infection after ozone exposure: sex differences.surfactant protein a (sp-a) enhances phagocytosis of bacteria, including klebsiella pneumoniae, by alveolar macrophages. ozone, a major air pollutant, can cause oxidation of surfactant and may influence lung immune function. immune function may also be affected by sex-specific mechanisms. we hypothesized that ablation of sp-a has a negative impact on the susceptibility of mice to klebsiella pneumoniae infection after ozone exposure, and that sex differences in the effect of ozone do exist.200819055785
antimicrobial and antifungal activities of the extracts and essential oils of bidens tripartita.the aim of this study was to determine the antibacterial and antifungal properties of the extracts, subextracts and essential oils of bidens tripartita flowers and herbs. in the study, twelve extracts and two essential oils were investigated for activity against different gram-positive bacillus subtilis, micrococcus luteus, staphylococcus aureus, gram-negative bacteria escherichia coli, e. coli (beta-laktamase+), klebsiella pneumoniae (esbl+), pseudomonas aeruginosa and some fungal organisms can ...200819056546
[biochemical and bacteriological investigation of six cases of purple urine bag syndrome (pubs) in a geriatric ward for dementia].purple urine bag syndrome is a condition in which the urinary catheter bag turns purple. a tryptophan-indigo hypothesis has been proposed as the mechanism of pubs, in which bacterial decomposition of tryptophan in gut associated with chronic constipation, bacterial overgrowth in the urinary tract and alkaline urine causes production of indigo and discoloration. we considered that further investigation of cases was needed.200819057104
first characterization and emergence of shv-60 in raw milk of a healthy cow in japan.during monitoring of raw milk samples from healthy cows for the presence of antibiotic resistant bacteria, one isolate of klebsiella pneumoniae strain huf-100 was found to be resistant to oxyimino-cephalosporins and aztreonam. it was found to carry a chromosomally-encoded extended-spectrum beta-lactamase that has not been described previously, namely shv-60. thus, it must be expected that this strain will spread further among food-producing animals and thereby constitute a reservoir of this resi ...200819057150
colonization of the respiratory tract by drug-resistant bacteria in hiv-infected children and prior exposure to antimicrobials.the aim of this short communication is to assess colonization by mrsa, penicillin-resistant pneumococci (prp), fluconazole-resistant (flu-r) candida albicans (ca) and non-albicans candida (nac), and ciprofloxacin-resistant e. coli with regard to immune recovery due to cd4 t-cell increase depending on the duration of highly active antiretroviral therapy (haart). prior exposure to oral cephalosporins (p<0.01) was significantly related to mrsa colonization. penicillin-resistant pneumococci were mor ...200819043382
the major surface-associated saccharides of klebsiella pneumoniae contribute to host cell association.analysing the pathogenic mechanisms of a bacterium requires an understanding of the composition of the bacterial cell surface. the bacterial surface provides the first barrier against innate immune mechanisms as well as mediating attachment to cells/surfaces to resist clearance. we utilised a series of klebsiella pneumoniae mutants in which the two major polysaccharide layers, capsule and lipopolysaccharide (lps), were absent or truncated, to investigate the ability of these layers to protect ag ...200819043570
[antagonistic properties of enterococci isolated from gastrointestinal tract of long-livers].the strains with antagonistic activity against indicator strains pseudomonas aeruginosa, proteus vulgaris, staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebsiella pneumonia, salmonella enterica were revealed among 58 enterococcus strains isolated from gastrointestinal tract of long-livers. the influence of different components of mrs medium on the antagonistic effect was established. antagonistic activity remained on the medium without glucose in 36% of strains, on the me ...200819044009
hyperammonemic encephalopathy caused by urinary tract infection with urinary retention. 200819046708
a case of haemoptysis. 200819046714
capsule polysaccharide is a bacterial decoy for antimicrobial peptides.antimicrobial peptides (aps) are important host weapons against infections. nearly all aps are cationic and their microbicidal action is initiated through interactions with the anionic bacterial surface. it is known that pathogens have developed countermeasures to resist these agents by reducing the negative charge of membranes, by active efflux and by proteolytic degradation. here we uncover a new strategy of resistance based on the neutralization of the bactericidal activity of aps by anionic ...200819047754
functional behaviour of polyethylene-zno nanocomposites.hybrid inorganic-organic nanocomposite materials are of current interest because of their multifunctionality, ease of processability, and potential for large-scale manufacturing. the focus of this study is to ascertain the functional properties of the commodity plastic impregnated with zno/starch nanocomposites. zno/starch nanocomposites was prepared by a simple process using zinc nitrate and sodium hydroxide as precursor and soluble starch as stabilizing agent. the peak obtained in terms of wav ...200819049187
[occurrence of ampc-positive klebsiella pneumoniae strains in patients with haematological malignancies].currently, important bacterial beta-lactamases of increasing clinical significance include ampc enzymes. the aim was to assess their occurrence in klebsiella pneumoniae strains isolated from patients with haematological malignancies in a prospective study.200819051165
synthesis and antimicrobial activity of some novel oxadiazole derivatives.a series of 5-(3'-oxo-6'-(substituted aryl)-2',3',4',5'-tetrahydropyridazin-2'-ylmethyl )-2-substituted 1,3,4-oxadiazole has been synthesized. appropriate aromatic hydrocarbon reacts with succinic anhydride in the presence of aicl3 to yield beta-aroyl propionic acid (1a). the corresponding acid is cyclized with hydrazine hydrate to give 6-(substituted aryl)-2,3,4,5-tetrahydro-3-pyridazinone (1b). this ntermediate after reaction with ethyl bromoacetate, was hydrazinolyzed into 3-oxo-6-(substitute ...200819051585
phytochemical and comparative antibacterial studies on the crude ethanol and aqueous extracts of the leaves of lecaniodiscus cupanoides planch (sapindaceae).phytochemical investigation was carried out on the leaves of lecaniodiscus cupanoides planch (sapindaceae). the diameter of the zones of inhibition of the 90% ethanol and aqueous extracts of the leaves were compared in order to determine the relative activity of the extracts against the tested microorganisms and also to verify its claimed ethnomedicinal use in the treatment of microbial infections. phytochemical tests were carried out employing standard procedures. the antimicrobial activity of ...200819051603
multiplex pcr for bla(ctx-m) & bla(shv) in the extended spectrum beta lactamase (esbl) producing gram-negative isolates.phenotyping is commonly used for detection of extended spectrum beta lactamase (esbl) production in gram-negative isolates. esbls are mainly coded for by three important genes, namely bla(tem), bla(shv) and bla(ctx-m). in this study we used a multiplex pcr as a rapid method to identify two common genes (bla(ctx-m) & bla(shv)) responsible for extended spectrum beta lactamase production in members of enterobacteriaceae family isolated from different clinical samples from a specialty hospital at ch ...200819052344
donovanosis with auto-amputation of penis in a hiv-2 infected person.donovanosis is a slowly progressive, granulomatous ulcerative disease , caused by klebsiella (calymmatobacterium) granulomatis. the disease is known to persist for years together, leading to complications. a male patient aged 30 years with underlying hiv-2 infection presented to the department of std with painful ulceration over the genital region of 5 months duration, with absence of penis. tissue smear from the ulcer and histopathological examination revealed large histiocytes with intracellul ...200819052412
antibiotic resistance in gram-negative bacilli: a singapore perspective.antibiotic resistance in gram-negative bacilli is an area of increasing importance. this prospective study was performed to survey antibiotic resistance in escherichia coli (e. coli), klebsiella spp., pseudomonas aeruginosa and acinetobacter spp. over a 1-year period.200819037514
ertapenem for treatment of extended-spectrum beta-lactamase-producing and multidrug-resistant gram-negative bacteraemia.imipenem and meropenem are treatment of choice for extended-spectrum betalactamase (esbl)-positive gram-negative bacteraemia. they may select for carbapenemresistant acinetobacter baumannii and pseudomonas aeruginosa; ertapenem may not do so as it is inactive against these bacteria. clinical efficacy of ertapenem in esbl-producing gramnegative bacteraemia is limited.200819037516
antibacterial activity of cefditoren against major community-acquired respiratory pathogens recently isolated in italy.in this study we evaluated the in vitro activities of cefditoren--a broad-spectrum oral cephalosporin--and other comparator agents against 2,396 fresh isolates from community-acquired respiratory tract infections, collected from 6 clinical italian microbiology laboratories. on penicillin-susceptible pneumococci and streptococcus pyogenes, cefditoren demonstrated to be the most active antibiotic (mic(90)values of 0.03 and 0.06 mg/l respectively), showing only a slight decrease in potency on penic ...200819028617
in vitro activity of tigecycline: mics, mbcs, time-kill curves and post-antibiotic effect.the minimum inhibitory concentrations (mics), minimum bactericidal concentrations (mbcs), time-kill curves, and postantibiotic effect (pae) of tigecycline, the first in the glycylcycline class of antibiotics, were evaluated. mics were determined against 749 clinical isolates. time-kill curves were performed against two isolates each of enterococcus faecalis, mssa, and mrsa. the presence of pae, against the same isolates, was investigated. mic(90)s (microg/ml) were the following: escherichia coli ...200819028619
risk factors in community-acquired/onset urinary tract infections due to extended-spectrum beta-lactamase-producing escherichia coli and klebsiella pneumoniae.we investigated the risk factors for community acquired/onset urinary tract infections due to extended spectrum beta-lactamase (esbl)-positive escherichia coli or klebsiella pneumoniae in 62 patients who were followed-up from august 1, 2003 to september 1, 2006. sixty patients with community-acquired urinary tract infections caused by esbl-negative e. coli or k. pneumoniae who were followed-up during the same dates were included as a control group. age (> or =65 or <65 years old), sex, bladder c ...200819028620
nosocomial spread of arma-mediated high-level aminoglycoside resistance in enterobacteriaceae isolates producing ctx-m-3 beta-lactamase in a cancer hospital in bulgaria.1,310 enterobacteriaceae, 242 pseudomonas aeruginosa and 97 acinetobacter baumannii clinical isolates collected at a cancer hospital in bulgaria were screened for the presence of 16s rrna methylases. the arma methylase gene was identified in 20 (1.5%) enterobacteriaceae (7 klebsiella pneumoniae, 3 escherichia coli, 3 serratia marcescens, 3 citrobacter freundii , 3 enterobacter cloacae and 1 klebsiella oxytoca). arma-mediated aminoglycoside resistance was transferable by conjugation and carried b ...200819028622
prevalence and genetic characterization of blactx-m among klebsiella pneumoniae isolates collected in an intensive care unit in brazil.thirteen (44.8%) ctx-m-2-producing k. pneumoniae clinical isolates were identified among 29 strains collected from single patients with serious infection hospitalized in an intensive care unit of a tertiary hospital located in são paulo, brazil. these isolates belonged to 9 different typing clusters and showed great diversity of plasmid content. their bla(ctx-m-2)was carried in an iscr1/sul1-type integron structure located in transferable plasmids of different sizes or in the chromosome.200819028623
effect of clarithromycin on lung inflammation and alveolar macrophage function in klebsiella penumoniae b5055-induced acute lung infection in balb/c mice.acute lung injuries due to acute lung infections remain the major cause of mortality. thus antibiotics with immunomodulatory and anti-inflammatory activities ,regardless of their antibacterial properties, will help to overcome acute lung infection-induced injuries. the macrolide antibiotics have been shown to possess these properties. clarithromycin has been shown to possess anti-inflammatory properties in chronic inflammatory conditions. so we evaluated the anti-inflammatory effect of clarithro ...200819028625
frequency of renal stone disease in patients with urinary tract infection.to determine incidence of renal stone disease in patients with urinary tract infection, this cross sectional study was conducted at surgical unit of district head quarters hospital, charsadda over a period of one year from january to december 2005.200819024188
aerobic vaginal pathogens and their sensitivity pattern.the vaginal flora is a complicated environment, containing dozens of microbiological species in variable quantities and relative proportions. the frequent cause of vaginal discharge is an infection or colonization with different microorganisms. some pathologic conditions causing vaginitis are well defined yet, 7-72% of women with vaginitis may remain undiagnosed and such forms of abnormal vaginal flora neither considered as normal, nor can be called bacterial vaginosis have been termed as 'inter ...200819024202
antibacterial activity of acalypha indica l.hexane, chloroform, ethyl acetate and methanol extracts from the leaves of acalypha indica were tested against gram-positive (staphylococcus aureus, staphylococcus epidermidis, bacillus cereus, streptococcus faecalis) and gram-negative (klebsiella pneumoniae, escherichia coli, proteus vulgaris, pseudomonas aeruginosa) bacteria. all the extracts exhibited antibacterial activity against gram-positive organisms with minimum inhibitory concentrations (mic) between 0.156 to 2.5 mg/ml. among the gram- ...200819024213
[a case of klebsiella pneumoniae outbreak in a newborn intensive care unit].to investigate and take a case study on a klebsiella pneumoniae outbreak in a newborn intensive care unit (nicu).200819024381
[susceptibilities of bacteria isolated from patients with lower respiratory infectious diseases to antibiotics (2005)].from october 2005 to september 2006, we collected the specimen from 366 patients with lower respiratory tract infections in 12 institutions in japan, and investigated the susceptibilities of isolated bacteria to various antibacterial agents and patients' characteristics. of 411 strains that were isolated from specimen (mainly from sputum) and assumed to be bacteria causing in infection, 406 strains were examined. the isolated bacteria were: staphylococcus aureus 70, streptococcus pneumoniae 85, ...200819024644
[nationwide susceptibility surveillance of ciprofloxacin and various parenteral antimicrobials against bacteria isolated from patients with severe infections--third ciproxan injection special survey (2005)].we conducted 3 nationwide surveillance studies between 2001 and 2005 at 39 participating institutions throughout japan according to the special survey plan to investigate susceptibility to ciprofloxacin (cpfx) and various parenteral antimicrobials using clinical isolates from patients with severe infection during the reexamination period of parenteral cpfx. results of the first special survey (2001) were already reported in this journal. the current third special survey (2005) was conducted at 3 ...200819024645
antimicrobial drug resistance in taiwan.antimicrobial resistance is a major health problem worldwide, but marked variations in the resistance profiles of bacterial pathogens are found between countries and in different patient settings, especially in asia. in taiwan, the strikingly high prevalence of resistance to macrolides and streptogramins in clinical isolates of gram-positive bacteria correlates with the widespread use of these agents in the medical and farming communities. the relatively low proportion of enterococci that are re ...200819013350
nationwide surveillance in taiwan of the in-vitro activity of tigecycline against clinical isolates of extended-spectrum beta-lactamase-producing enterobacteriaceae.tigecycline in-vitro surveillance in taiwan (tist), initiated in 2006, is a nationwide surveillance programme designed to monitor longitudinally the in-vitro activity of tigecycline against commonly encountered resistant bacteria. this study compared the in-vitro activity of tigecycline against clinical isolates of resistant gram-negative bacteria determined by the broth microdilution and etest methods. a total of 622 isolates were collected from patients treated at 20 teaching hospitals. tigecy ...200819013351
functional nanoparticle-based proteomic strategies for characterization of pathogenic bacteria.although matrix-assisted laser desorption/ionization mass spectrometry (maldi ms) can be employed to rapidly characterize pathogenic bacteria, bacterial cultures are generally required to obtain sufficient quantities of the bacterial cells prior to maldi ms analysis. if this time-consuming step could be eliminated, the length of time required for identification of bacterial strains would be greatly reduced. in this paper, we propose an effective means of rapidly identifying bacteria--one that do ...200819007241
towards understanding the nitrogen signal transduction for nif gene expression in klebsiella pneumoniae.in the diazotroph klebsiella pneumoniae, the nitrogen sensory protein glnk mediates the cellular nitrogen status towards the nifl/nifa system that regulates transcription of the nitrogen fixation genes in response to ammonium and molecular oxygen. to identify amino acids of glnk essential for this signal transduction by protein-protein interaction, we performed random point mutagenesis by pcr amplification under conditions of reduced taq polymerase fidelity. three thousand two hundred mutated gl ...200819016838
therapeutic potential of bacteriophage in treating klebsiella pneumoniae b5055-mediated lobar pneumonia in mice.klebsiella pneumoniae causes infections in humans especially in immunocompromised patients. about 80 % of nosocomial infections caused by k. pneumoniae are due to multidrug-resistant strains. the emergence of antibiotic-resistant bacterial strains necessitates the exploration of alternative antibacterial therapies, which led our group to study the ability of bacterial viruses (known as bacteriophages or simply phages) to treat mice challenged with k. pneumoniae. phage ss specific for k. pneumoni ...200819018021
a klebsiella pneumoniae sputum culture isolate from china carrying blaoxa-1, blactx-m-55 and aac(6')-ib-cr. 200819018035
bacterial aetiology and outcome in children with severe pneumonia in uganda.pneumonia is a major cause of morbidity and mortality in the 'under-5s' and in uganda accounts for 10-30% of childhood deaths. antibiotic resistance is increasing.200819021940
emergence of extensively drug-resistant and pandrug-resistant gram-negative bacilli in europe.international and local surveillance networks as well as numerous reports in the biomedical literature provide evidence that the prevalence of antibiotic resistant gram-negative bacteria is escalating in many european countries. furthermore, isolates characterised as multidrug-resistant (i.e. resistant to three or more classes of antimicrobials), extensively drug resistant (i.e. resistant to all but one or two classes) or pandrug-resistant (i.e. resistant to all available classes) are increasing ...200819021957
increasing prevalence of esbl-producing enterobacteriaceae in europe.extended-spectrum beta-lactamases (esbls) have been increasingly reported in europe since their first description in 1983. during the 1990s, they were described mainly as members of the tem- and shv-beta-lactamase families in klebsiella pneumoniae causing nosocomial outbreaks. nowadays, they are mostly found in escherichia coli that cause community-acquired infections and with increasing frequency contain ctx-m enzymes. dissemination of specific clones or clonal groups and epidemic plasmids in c ...200819021958
pyogenic portal vein thrombosis as a reservoir of persistent septicemia after cyanoacrylate injection for bleeding gastric varices.gastric variceal bleeding is a serious complication of liver cirrhosis. a recent consensus suggested that endoscopic injection of tissue glue for gastric variceal obliteration (gvo) should be the first choice for treatment of acute gastric variceal bleeding. following the widespread use of gvo, more severe complications such as needle cementation, fistula formation, embolic sequels, recurrent septicemia, etc., have been reported. we present the first case of gvo-complicated pyogenic portosplenic ...200819023208
emergence of extensive drug resistance (xdr) among gram-negative bacilli in south africa looms nearer. 200818928032
[qnr-type quinolone resistance in extended-spectrum beta-lactamase producing enterobacteria in abidjan, ivory coast].the aim of the study was to show the emergence of the qnr genes in extended spectrum beta-lactamases producing enterobacteria in abidjan between 2005 and 2006. the whole of 151 strains of extended spectrum beta-lactamases producing enterobacteria were studied: 64 escherichia coli, 66 klebsiella pneumoniae, seven klebsiella oxytoca and 14 enterobacter spp. isolated from various biological products and from in- and out-patients. the techniques of disks diffusion, double-disk synergy, e-test were r ...200818926644
childhood bacterial meningitis in ibadan, nigeria--antibiotic sensitivity pattern of pathogens, prognostic indices and outcome.bacterial meningitis remains a major cause of morbidity, mortality and neurodisability in childhood, particularly in the developing world where effective vaccines against the usual pathogens responsible for the disease are not in routine use. to describe the patterns and outcome of bacterial meningitis among children admitted into the university college hospital (uch), ibadan, nigeria. all children who satisfied the case definition for meningitis, admitted into the paediatric wards of the univer ...200818939404
[evaluation of elderly patients with community-acquired pneumonia admitted to our hospital, who have neurologic disease, such as late effects of cerebral strokes in the past and medical history].hospitalized cases of community-acquired pneumonia (cap) of the elderly with a history of neurologic diseases have not been examined in detail in the past.200818939411
antioxidant and antimicrobial effects of phenolic compounds extracts of northeast portugal honey.phenolic compounds of dark and clear honeys from trás-os-montes of portugal were extracted with amberlite xad-2 and evaluated for their antioxidant and antimicrobial activities. the antioxidant effect was studied using the in vitro test capacity of scavenge the 2,2-diphenyl-1-picryhydrazyl (dpph) free radical and of reducing power of iron (iii)/ferricyanide complex. the antimicrobial activity was screened using three gram-positive bacteria (bacillus subtilis, staphylococcus aureus, staphylococcu ...200818940227
novel microchip for in situ tem imaging of living organisms and bio-reactions in aqueous conditions.a novel and disposable microchip (k-kit) with sio(2) nano-membranes was developed and used as a specimen kit for in situ imaging of living organisms in an aqueous condition using transmission electron microscopy (tem) without equipment modification. this k-kit enabled the successful tem observation of living escherichia coli cells and the tellurite reduction process in klebsiella pneumoniae. the k. pneumoniae and saccharomyces cerevisiae can stay alive in k-kit after continuous tem imaging for u ...200818941693
nhsn annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the national healthcare safety network at the centers for disease control and prevention, 2006-2007.to describe the frequency of selected antimicrobial resistance patterns among pathogens causing device-associated and procedure-associated healthcare-associated infections (hais) reported by hospitals in the national healthcare safety network (nhsn).200818947320
clinical and molecular epidemiology of healthcare-associated infections due to extended-spectrum beta-lactamase (esbl)-producing strains of escherichia coli and klebsiella pneumoniae that harbor multiple esbl genes.to characterize healthcare-associated infections due to extended-spectrum beta-lactamase (esbl)-producing strains of escherichia coli and klebsiella pneumoniae that harbor multiple esbl genes, as opposed to a single esbl gene.200818947321
rhinoscleroma: a french national retrospective study of epidemiological and clinical features.rhinoscleroma (rs) is a rare, chronic, granulomatous disease of the upper respiratory tract that is associated with infection with klebsiella rhinoscleromatis. rs is more common in certain geographic regions than in others, but other risk factors and the pathogenesis of rs remain unclear.200818947330
[distribution and drug resistance of the isolated bacteria from children with acute respiratory infection].to study the distribution and drug resistance of the isolated bacteria from children with acute respiratory infection (ari) in dalian.200818947473
detection of protozoan and bacterial pathogens of public health importance in faeces of corvus spp. (large-billed crow).parasites and bacteria are reported in the faeces of birds in the current study. fresh faecal samples of the large-billed crow (corvus spp.) were collected from the study site at bangsar, an urban setting in kuala lumpur, malaysia. these samples were transported to laboratory and analysed for parasites and bacteria. pre-prepared xld agar plates were used for culturing the bacteria in the laboratory. using the api 20etm test strips, 9 different species of bacteria were identified belonging to the ...200818948884
a cluster of klebsiella pneumoniae bacteremia in a radiation oncology ward.we report an outbreak of genetically related strains of klebsiella pneumoniae bloodstream infection.200818834724
impact of a hospital-wide antimicrobial formulary intervention on the incidence of multidrug-resistant gram-negative bacteria.we examined the impact of an antimicrobial formulary change, based on reduction in third-generation cephalosporin use, on resistant gram-negative pathogens in a tertiary hospital. no significant changes were demonstrated in their incidence per 1000 patient-days. otherwise, there was a significant decrease in rate of extended-spectrum beta-lactamase (esbl)-producing klebsiella pneumoniae (63.1% to 52.5%, p = .04) and third-generation cephalosporin-resistant enterobacter species (31.4% to 25%, p = ...200818834728
in vitro pharmacodynamics of colistin against multidrug-resistant klebsiella pneumoniae.resistance to colistin is emerging in multidrug-resistant gram-negative bacteria and no solid pharmacodynamic data are available for colistin against klebsiella pneumoniae.200818922815
simple disk-based method for detection of klebsiella pneumoniae carbapenemase-type beta-lactamase by use of a boronic acid compound.a disk potentiation method using carbapenems as substrates and 3-aminophenyl boronic acid as an inhibitor was evaluated for the detection of klebsiella pneumoniae carbapenemase (kpc)-type beta-lactamases. when combined with nonsusceptibility to ertapenem, the method was easy to perform and reliably differentiated isolates producing kpc-type beta-lactamases from those producing other types of beta-lactamases.200818923005
the hpx genetic system for hypoxanthine assimilation as a nitrogen source in klebsiella pneumoniae: gene organization and transcriptional regulation.growth experiments showed that adenine and hypoxanthine can be used as nitrogen sources by several strains of k. pneumoniae under aerobic conditions. the assimilation of all nitrogens from these purines indicates that the catabolic pathway is complete and proceeds past allantoin. here we identify the genetic system responsible for the oxidation of hypoxanthine to allantoin in k. pneumoniae. the hpx cluster consists of seven genes, for which an organization in four transcriptional units, hpxde, h ...200818849434
different application volumes of ethyl-cyanoacrylate tissue adhesive can change its antibacterial effects against ocular pathogens in vitro.to analyze the antibacterial effects in vitro of ethyl-cyanoacrylate (ec) tissue adhesive in different application volumes.200818853314
[etiological bacterial factors of respiratory system infections in the intensive care unit surgery clinic].pneumonia is the most frequently occurring hospital-acquired infection in intensive care units (icu). the frequency of bacteriological pathogenic factors in the surgical icu and efficiency of empiric antibiotic therapy used in icu was assessed. the study included 239 icu patients in the i department of general surgery and gastroenterological surgery clinics of jagiellonian university hospital in 2006 year. the bronchoscope materials were worked out with the application of qualitative and quantit ...200818853659
prevalence of quinolone-resistant urinary tract infections in comanche county memorial hospital.urinary tract infection (uti) is not only a common outpatient but also the most frequently occurring nosocomial infection. the most common causative organisms of uti remain escherichia coli, nosocomial gram-negative bacilli, enteroccoci and candida. many physicians use quinolones as the agent of choice for treatment of uti. as calculated by the drug utilization data of 2007, the rate of quinolone use by physicians for uti is 48%. this study was performed to determine the prevalence of quinolone- ...200818856050
locked nucleic acid pentamers as universal pcr primers for genomic dna amplification.multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods. however, the multiplexing method currently used requires precise handiwork and many complicated steps, making a new, simpler technique desirable. oligonucleotides containing locked nucleic acid residues are an attractive tool because they have strong affinities for their complem ...200819002243
atypical mycobacteriosis of the larynx: an unusual clinical presentation secondary to steroids inhalation.vocal cords stiffness can be associated with a variety of etiologic agents, but it is rarely seen with atypical mycobacteria, for example, mycobacterium avium complex. we report a case of a 35-year-old white woman who is and was maintained on inhaled steroids. she presented with hoarseness and low-grade fever, but her medical history was otherwise unremarkable. laryngoscopy revealed bilateral scarring of the vocal cords. vocal cord biopsies were performed. histologic examination revealed unremar ...200818995207
a study on nosocomial pathogens in icu with special reference to multiresistant acinetobacter baumannii harbouring multiple plasmids.antibiotic resistant bacterial nosocomial infections are a leading problem in intensive care units (icu). present investigation was undertaken to know antibiotic resistance in acinetobacter baumannii and some other pathogens obtained from clinical samples from icu causing nosocomial infections. special emphasis was given on plasmid mediated transferable antibiotic resistance in acinetobacter.200819001682
protective efficacy of emblica officinalis against klebsiella pneumoniae induced pneumonia in mice.emblica officinalis (amla), which is a good source of vitamin c, has been shown to be beneficial due to its immune system enhancing property coupled with its tonifying and antiageing effect. the present study was conducted to evaluate the effect of e. officinalis feeding on the susceptibility of experimental mice to respiratory tract infection induced by klebsiella pneumoniae.200819001683
[analysis of antibiotic consumption and microorganism resistance changes].summary objective: the main goal of this study was to evaluate the variation of antibiotic consumption and relation between antibiotic consumption and microorganism resistance.200819001833
role of human cd36 in bacterial recognition, phagocytosis, and pathogen-induced jnk-mediated signaling.scavenger receptor cd36 mediates staphylococcus aureus phagocytosis and initiates tlr2/6 signaling. we analyzed the role of cd36 in the uptake and tlr-independent signaling of various bacterium, including escherichia coli, klebsiella pneumoniae, salmonella typhimurium, s. aureus, and enterococcus faecalis. expression of human cd36 in hela cells increased the uptake of both gram-positive and gram-negative bacteria compared with the control mock-transfected cells. bacterial adhesion was associated ...200818981136
klebsiella pneumoniae liver abscess: an emerging problem in north america.background: before the 1980s, escherichia coli was the most common cause of pyogenic liver abscess, but more recently, klebsiella pneumoniae has emerged as the most common organism in the united states and taiwan. objective: our goal is to present a case of k. pneumoniae liver abscess (kla) and review the risk factors, presenting symptoms, complications, and treatment of this disease that is emerging in north america. case report: we present a patient who was found to have kla complicated by bac ...200818993020
bacteriology of non-surgical wound infections in ibadan, nigeria.previous studies done on wound infections in this environment had been mostly on the surgical variety rather than the non-surgical. however,few studies available on the non-surgical type have indicated that changes do occur in the pattern and antibiogram of the bacterial isolates of these non-surgical wound infections. this study was therefore designed to investigate the bacteriology of non-surgical wound infections in this environment. a retrospective review of seven hundred and fifty four case ...200818982819
antimicrobial susceptibility pattern of microorganisms involved in the pathogenesis of surgical site infection (ssi); a 1 year of surveillance.the aim of this study is to identify the antibiotic sensitivity pattern of pathogens involved in the process of surgical site infection, in surgical wards. changes made in the pattern of antibiotic use will result in different microorganism susceptibility patterns, which needs correct determination for precise empiric antibiotic therapy. one thousand patients (62% men and 38% women, 18- 74-years-old, with mean age 43 +/- 8)) who underwent surgical treatment, in alzahra university hospital, isfah ...200818983037
spectrum of organisms causing peritonitis in peritoneal dialysis patients--experience from bangladesh.in the present study, organisms responsible for peritonitis and their sensitivity to antibiotics were evaluated in peritoneal dialysis (pd) patients in bangladesh. we collected pd effluent from 100 peritonitis cases and sent samples to the laboratory for gram stain and cytology. cultures used direct inoculation of pd fluid in plate media and broth media simultaneously. organisms were isolated by gram stain in 60% of cases. cell counts showed a mean of 700 (range: 90-7000) white blood cells per m ...200818985999
time-dependent effects of klebsiella pneumoniae endotoxin on the telithromycin pharmacokinetics in rats; restoration of the parameters in 96-hour kplps rats to the control levels.it has been reported that telithromycin is primarily metabolized via hepatic cyp3a4 and 3a1/2 in humans and rats, respectively, and that the protein expression of hepatic cyp3a subfamily significantly decreased (59.1% decrease) in 24-h kplps rats (lipopolysaccharide derived from klebsiella pneumoniae; the protein expression was measured 24h after kplps administration) compared with that in control rats, but restored to that in control rats in 96-h kplps rats.200818976719
[tracheopathia osteoplastica].report a case of tracheopathia osteoplastica and describe from a literature analysis the main clinical, radiological, and therapeutic features of this rare disease based on literature review.200818614154
transfer of antibiotic multiresistant plasmid rp4 from escherichia coli to activated sludge bacteria.in situ transfer of a self-transmissible, antibiotic-multiresistant plasmid rp4 from a laboratory escherichia coli strain c600 to indigenous activated sludge bacteria was investigated using filter mating. the transfer frequency of rp4 from the donor e. coli to the bacteria that was sampled from two wastewater treatment plants was 5.1x10(-2) to 7.5x10(-1) and 4.6x10(-3) to 1.3x10(-2)/potential recipient. the isolated transconjugants showed resistance to ap, km, and tc and the presence of a plasmi ...200818930008
klebsiella pneumoniae carbapenemase-producing organisms: an ounce of prevention really is worth a pound of cure. 200818973453
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