Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| regulation of acetamide utilization. | 1994 | 7765128 | |
| sulphur metabolism. | 1994 | 7765129 | |
| the early history. | 1994 | 7765130 | |
| nitrogen metabolite repression. | 1994 | 7765131 | |
| aspergillus nidulans as an experimental organism. | 1994 | 7765132 | |
| carbon catabolite repression. | 1994 | 7765133 | |
| regulation of gene expression by oxygen, phosphorus and ph. | 1994 | 7765134 | |
| genetic regulation of conidiation. | 1994 | 7765135 | |
| sexual sporulation. | 1994 | 7765136 | |
| control of cell growth. | 1994 | 7765137 | |
| ribosomes. | 1994 | 7765138 | |
| mitochondria. | 1994 | 7765139 | |
| penicillin biosynthesis. | 1994 | 7765141 | |
| carbon metabolism. | 1994 | 7765142 | |
| vectors for genetic manipulation. | 1994 | 7765143 | |
| homologous recombination. | 1994 | 7765145 | |
| translational suppression. | 1994 | 7765146 | |
| linkage map and locus list. | 1994 | 7765147 | |
| gene symbols. | 1994 | 7765148 | |
| media. | 1994 | 7765149 | |
| sources of strains, vectors, libraries. | 1994 | 7765150 | |
| how many 5s rrna genes and pseudogenes are there in aspergillus nidulans? | we have estimated the number of 5s rrna genes in aspergillus nidulans using two-dimensional agarose gel electrophoresis and hybridization to appropriate probes, representing the 5'-halves, the 3'-halves of the 5s rrna sequence and a sequence found at the 3'-end of all known a. nidulans pseudogenes (block c). we have found 23 5s rrna genes, 15 pseudogenes consisting of the 5'-half of the 5s rrna sequence (of which 3 are flanked by block c) and 12 copies of block c which do not seem to be in the v ... | 1994 | 7732763 |
| purification of arginase from aspergillus nidulans. | arginase (ec 3.5.3.1) of aspergillus nidulans, the enzyme which enables the fungus to use arginine as the sole nitrogen source was purified to homogeneity. molecular mass of the purified arginase subunit is 40 kda and is similar to that reported for the neurospora crassa (38.3 kda) and saccharomyces cerevisiae (39 kda) enzymes. the native molecular mass of arginase is 125 kda. the subunit/native molecular mass ratio suggests a trimeric form of the protein. the arginase protein was cleaved and pa ... | 1994 | 7732765 |
| molecular biological and biochemical aspects of fungal dimorphism. | 1994 | 7722802 | |
| cdna structure and characterization of a kinesin-like protein from the silkworm bombyx mori. | we have isolated a 1224 bp cdna clone from a bombyx mori embryonic cdna library which contains sequences homologous to the kinesin-like protein gene, ncd, which is required for distribution of chromosomes at meiosis in drosophila melanogaster females. this clone includes both a microtubule motor and the atp-binding domains found in kinesin-like proteins. the motor domain is classified in the group of the bimc and cut7, which have a role in spindle formation during mitosis of aspergillus nidulans ... | 1994 | 7704303 |
| cellular effects of misscheduled brla, abaa, and weta expression in aspergillus nidulans. | asexual sporulation in the fungus aspergillus nidulans is controlled, in part, by a central regulatory pathway composed of the brla, abaa, and weta genes. the coding region of each of these genes was fused, in frame, to the threonine-inducible alcohol dehydrogenase promotor then stably incorporated into the a. nidulans genome at the argb locus. misscheduled expression of each of these developmental genes interfered with normal growth and sporulation. expression of brla or abaa terminated vegetat ... | 1994 | 7704830 |
| production and characterization of sterigmatocystin. | fourteen strains of aspergillus versicolor and 2 strains of a. nidulans were screened for sterigmatocystin (st) production on a semi-synthetic solid substrate by high performance liquid chromatography (hplc) analysis. two strains of a. versicolor producing st at 550.5 mg.kg-1 substrate and 1160.8 mg.kg-1 substrate were selected to inoculate 4 kg solid st-producing media. after 30 days stationary incubation at 28 degrees c in the dark, 2271.6 mg of pale-yellow needle-shaped crystals were isolated ... | 1994 | 7702759 |
| mitotic catastrophe is the mechanism of lethality for mutations that confer mutagen sensitivity in aspergillus nidulans. | we have examined the consequences of treatment with dna-damaging agents of uvs mutants and the bimd6 mutant of aspergillus nidulans. we first established that wild-type aspergillus undergoes a cell cycle delay following treatment with the dna-damaging agents methyl methanesulfonate (mms) or ultraviolet light (uv). we have also determined that strains carrying the bimd6, uvsb110, uvsh77, uvsf201 and the uvsc114 mutations, all of which cause an increased sensitivity to dna-damaging agents, undergo ... | 1994 | 7506362 |
| genotoxic activity of mebendazole in aspergillus nidulans. | mebendazole is an anthelmintic drug widely used in cuba and in mexico. its interaction with tubulin interferes with the assemblage of the mitotic apparatus in the parasite cells, thus suggesting a possible genotoxic activity leading to chromosomal malsegregation. the heterozygous diploid strain d30 of aspergillus nidulans was used to establish the ability of mebendazole to induce mitotic recombination and/or chromosomal non-disjunction, and the haploid strain fgsc #219 of a. nidulans was used to ... | 1994 | 7510023 |
| the genetic activity of 6-n-hydroxylaminopurine in aspergillus nidulans. | the activity of a base analog (6-n-hydroxylaminopurine, hap) has been tested on aspergillus nidulans. in germinating haploid conidia hap is a strong mutagen, while it does not have any activity in resting conidia. moreover, hap does not increase the frequency of recombination in germinating conidia. the mutagenic activity of this base analog has also been tested in diploid conidia of a. nidulans; in fact, it has been shown (pavlov et al., 1991) that the hap-induced frequency of heteroallelic rec ... | 1994 | 7510841 |
| a test for uniparental disomy in saccharomyces cerevisiae. | uniparental disomy is a condition in a diploid organisms where one parental chromosome is absent and its homolog from the other parent duplicated. it can be a cause of genetic somatic disease in mammals because of imprinting. imprinting creates a sex-specific pattern of epigenetic gene inactivation at least in mammals and, consequently, a complete set of both maternal and paternal chromosomes is required for normal development. moreover, it has been shown for several types of tumors that recessi ... | 1994 | 7512218 |
| mycoflora of post-harvest maize and wheat grains and the implication of their contamination by molds. | thirteen fungi including toxigenic aspergillus nidulans and a. clavatus were isolated from the grains. the isolated fungi grew well at 25-30 degrees c. a. clavatus and a. nidulans were grown in liquid maize yeast extract medium and wheat yeast extract medium. both fungi produced amylases on the two media and on the basal medium at 30 degrees c. during incubation more total reducing sugars were detected in maize grains than in wheat while non-reducing sugars were detected than reducing sugars in ... | 1994 | 7518052 |
| cell cycle-dependent expression of nek2, a novel human protein kinase related to the nima mitotic regulator of aspergillus nidulans. | the serine/threonine protein kinase nima of aspergillus nidulans is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. here, we report the isolation of complementary dnas encoding nek2 and nek3, two novel human protein kinases structurally related to nima. sequence comparisons revealed several unique features which may define a family of nima-related protein kinases. nek2 was chosen for further study since it represents the closest known mammal ... | 1994 | 7522034 |
| molecular and cellular parameters controlling the immunogenicity of foreign b- or t-cell epitopes expressed by recombinant vectors. | extensive work is being performed to develop live recombinant bacterial vaccines. the use of non-pathogenic bacteria or attenuated strains derived from pathogens may allow protection against the pathogen and at the same time induce immunity against one or several foreign antigens expressed by the recombinant micro-organism. several bacteria such as attenuated salmonella or bcg have been used successfully in several experimental models to induce protective immune responses against several pathoge ... | 1994 | 7525391 |
| nitrite reductase gene cloning of amycolatopsis mediterranei u-32. | southern blot analysis showed great homology existed between niad (nr gene) of aspergillus nidulans and a. mediterranei u-32 chromosome dna. a 5.0kb psti fragment from a. mediterranei u-32 complementary to a. nidulans niad gene was cloned in e. coli nm522 using niad as a probe. an identical dna band was observed through back-hybridization of the cloned dna fragment to psti digest of a. mediterranei u-32 chromosome dna. its 2.1 kb smai-ecorv fragment can only hybridize with total rna from nitrate ... | 1994 | 7534488 |
| based on biochemical and physiological behavior, where is aspergillus egyptiacus better placed? | physiological and biochemical properties were tested in 45 isolates of aspergillus egyptiacus (16 isolates), emericella nidulans (16) and aspergillus versicolor (13). the three fungal species exhibited common and similar features. the big similarity between a. egyptiacus and e. nidulans was greater than between a. egyptiacus and a. versicolor. it included the inability to produce base either from sodium citrate or lactic acid media, growth at 45 degrees c (thermophilicity), and production of ver ... | 1994 | 7537240 |
| monoclonal antibodies against a 97-kilodalton antigen from aspergillus flavus. | we prepared a panel of five monoclonal antibodies (mabs) directed against aspergillus flavus that all reacted against one 97-kda antigen by western blot (immunoblot). flow cytometry demonstrated that these antibodies bound (in increasing degrees) to all morphologic stages of a. flavus growth: conidia, swollen conidia, and hyphae. cross-reactivity among species was examined by enzyme-linked immunosorbent assay of fungal culture filtrates. four mabs reacted with 10 of 11 a. flavus isolates, and th ... | 1994 | 7496924 |
| transport of lactate and its regulation in saccharomyces cerevisiae mutants deficient in specific metabolic steps. | 1994 | 8550003 | |
| genetic and molecular characterisation of purine permease genes of aspergillus nidulans reveals a novel family of transporters conserved in prokaryotes and eukaryotes. | the ascomycete fungus aspergillus nidulans can utilize purines (adenine, guanine, hypoxanthine, xanthine, and uric acid) as sole nitrogen sources [1]. the expression of most structural genes involved in the pathway of purine uptake and catabolism is subject to uric acid induction, mediated by the product of the positive regulatory gene uay, and to nitrogen metabolite repression, mediated by the product of the general, positive-acting, gata-like transcription factor, encoded by the area gene [1]. | 1994 | 8550005 |
| structure-function analysis of the proline permease (prnb) of the filamentous fungus aspergillus nidulans. | 1994 | 8550020 | |
| cloning and characterisation of the cytochrome c gene of aspergillus nidulans. | the cytochrome c gene (cyca) of the filamentous fungus aspergillus nidulans has been isolated and sequenced. the gene is present in a single copy per haploid genome and encodes a polypeptide of 112 amino acid residues. the nucleotide sequence of the a. nidulans cyca gene shows 87% identity to the dna sequence of the neurospora crassa cytochrome c gene, and approximately 72% identity to the sequence of the saccharomyces cerevisiae iso-1-cytochrome c gene (cyc1). the s. cerevisiae cyc1 gene was us ... | 1994 | 8277943 |
| relationship between zinc content and dna-binding activity of the dna-binding motif of the transcription factor alcr in aspergillus nidulans. | the transcription factor alcr of the ethanol utilisation pathway in aspergillus nidulans contains a zinc binuclear motif (cysx2cysx6cysx16cysx2cysx6cys), within the dna-binding domain located in the n-terminal region of the alcr protein. specific targets have been localised in the promoter of the alcr gene, involved in the autoregulation process, and in the promoter of the structural gene alca (encoding alcohol dehydrogenase i), which is also under the control of alcr. the dna-binding domain has ... | 1994 | 8277945 |
| analysis of the regulation of penicillin biosynthesis in aspergillus nidulans by targeted disruption of the acva gene. | to analyse the regulation of the biosynthesis of the secondary metabolite penicillin in aspergillus nidulans, a strain with an inactivated acva gene produced by targeted disruption was used. acva encodes delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine synthetase (acvs), which catalyses the first step in the penicillin biosynthetic pathway. to study the effect of the inactivated acva gene on the expression of acva and the second gene, ipna, which encodes isopenicillin n synthase (ipns), a. nidul ... | 1994 | 8277946 |
| isolation and characterization of two chitin synthase genes from aspergillus nidulans. | two chitin synthase genes, designated chsa and chsb, were isolated from aspergillus nidulans with the saccharomyces cerevisiae chs2 gene as the hybridization probe. nucleotide sequencing showed that chsa and chsb encoded polypeptides consisting of 1013 and 916 amino acid residues, respectively; the hydropathy profiles of the enzymes were similar to those of other fungal chitin synthases. northern analysis indicated that both genes were transcribed, suggesting that cellular chitin in a. nidulans ... | 1994 | 7765508 |
| genetic modification of an echinocandin b-producing strain of aspergillus nidulans to produce mutants blocked in sterigmatocystin biosynthesis. | the production of echinocandin b (ecb), a lipopolypeptide used for chemical manufacture of the anti-candida agent cilofungin, was accomplished by fermentation using a strain of aspergillus nidulans. in addition to ecb, this fermentation also produces a significant amount of sterigmatocystin (st), a potent carcinogen structurally related to the aflatoxins. mutants blocked in the st biosynthetic pathway were created by genetic modification of the polyploid production strain c747. the following ste ... | 1994 | 7765669 |
| isolation of a chitin synthase gene (chsc) of aspergillus nidulans. | we isolated a class i chitin synthase gene (chsc) from aspergillus nidulans. expression of this gene was confirmed by northern analysis and by sequencing of the pcr-amplified dna fragments from cdna. chsc disruptants showed no difference of morphology in the asexual cycle and no difference of growth rate compared to a wild-type strain. | 1994 | 7765719 |
| two novel deduced serine/threonine protein kinases from saccharomyces cerevisiae. | we have cloned genes for two closely related protein kinase (pk) homologues from saccharomyces cerevisiae. sequence alignment of the kinase domain with previously characterized pk reveals the highest degree of similarity with second messenger-regulated kinases. rck1 encodes a 58-kda protein, and is weakly expressed. rck2 encodes a 65-kda protein, and transcripts from this gene are readily detected. rck1 has been mapped to the l arm of chromosome vii, and rck2 to chromosome xii. disruption of the ... | 1994 | 8112585 |
| induction of pseudohyphal growth by overexpression of phd1, a saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. | when starved for nitrogen, mata/mat alpha cells of the budding yeast saccharomyces cerevisiae undergo a dimorphic transition to pseudohyphal growth. a visual genetic screen, called phd (pseudohyphal determinant), for s. cerevisiae pseudohyphal growth mutants was developed. the phd screen was used to identify seven s. cerevisiae genes that when overexpressed in mata/mat alpha cells growing on nitrogen starvation medium cause precocious and unusually vigorous pseudohyphal growth. phd1, a gene whos ... | 1994 | 8114741 |
| fungal catabolic gene regulation: molecular genetic analysis of the amds gene of aspergillus nidulans. | aspergillus nidulans is an excellent experimental organism for the study of gene regulation. genetic and molecular analyses of trans-acting and cis-acting mutations have revealed a complex pattern of regulation involving multiple independent controls. expression of the amds gene is regulated by the facb and amda genes which encode positively acting regulatory proteins mediating a major and a minor form of acetate induction respectively. the product of the amdr gene mediates omega amino acid indu ... | 1993 | 8119589 |
| the macronuclear gamma-tubulin-encoding gene of euplotes octocarinatus contains two introns and an in-frame tga. | the gamma-tubulin (gamma-tub)-encoding gene (gamma-tub) of euplotes octocarinatus was amplified from macronuclear dna with the help of the polymerase chain reaction (pcr) and sequenced. the polypeptide deduced from the gene consists of 462 amino acids (aa). it shares 61% aa identity with the aspergillus nidulans gamma-tub. the gene contains an in-frame tga codon and two small pre-mrna introns (36 and 26 bp). we suggest that the tga, like tga codons in the pheromone-encoding genes of e. octocarin ... | 1993 | 8294024 |
| genesis of eukaryotic transcriptional activator and repressor proteins by splitting a multidomain anabolic enzyme. | the genes necessary for the correctly regulated catabolism of quinate in aspergillus nidulans and neurospora crassa are controlled at the level of transcription by a dna-binding activator protein and a repressor protein that directly interact with one another. the repressor protein is homologous throughout its length with the three c-terminal domains of a pentafunctional enzyme catalysing five consecutive steps in the related anabolic shikimate pathway. we now report that the activator protein i ... | 1993 | 8294040 |
| molecular organization of the escherichia coli gab cluster: nucleotide sequence of the structural genes gabd and gabp and expression of the gaba permease gene. | we have determined the nucleotide sequences of two structural genes of the escherichia coli gab cluster, which encodes the enzymes of the 4-aminobutyrate degradation pathway: gabd, coding for succinic semialdehyde dehydrogenase (ssdh, ec 1.2.1.16) and gabp, coding for the 4-aminobutyrate (gaba) transport carrier (gaba permease). we have previously reported the nucleotide sequence of the third structural gene of the cluster, gabt, coding for glutamate: succinic semialdehyde transaminase (ec 2.6.1 ... | 1993 | 8297211 |
| induction of glucose oxidase, catalase, and lactonase in aspergillus niger. | the induction of glucose oxidase, catalase, and lactonase activities was studied both in wild-type and in glucose oxidase regulatory and structural mutants of aspergillus niger. the structural gene for glucose oxidase was isolated and used for northern analysis and in transformation experiments using various gox mutations. wild-type phenotype could be restored in the glucose oxidase-negative mutant (goxc) by transformation with the structural gene. we conclude, therefore, that the goxc marker wh ... | 1993 | 8299156 |
| co-transformation with autonomously-replicating helper plasmids facilitates gene cloning from an aspergillus nidulans gene library. | autonomously-replicating, marker-less "helper" plasmids were added to transformations of aspergillus nidulans with plasmids which normally transform by chromosomal integration. this resulted in as much as a 200-fold increase in transformation efficiency. recovery of autonomously-replicating plasmid co-integrates indicated that co-transformation involves recombination between integrating and helper plasmids, which occurs at a high frequency. increasing dna sequence-homology between pairs of plasm ... | 1993 | 8299174 |
| cloning and characterization of the abfb gene coding for the major alpha-l-arabinofuranosidase (abf b) of aspergillus niger. | based on amino-acid sequence data from aspergillus niger alpha-l-arabinofuranosidase b (abf b), and cyanogen bromide fragments derived thereof, deoxyoligonucleotide mixtures were designed to be employed as primers in a polymerase chain reaction (pcr) on a. niger genomic dna. this resulted in amplification of three related pcr products. the abfb gene encoding abf b was isolated from a genomic library using such an amplification product as a probe. a 5.1-kb bamhi fragment was subcloned to result i ... | 1993 | 8299175 |
| nucleotide sequence of the large mitochondrial rrna gene of penicillium chrysogenum. | the nucleotide sequence of a large rrna (1-rrna) gene and its flanking regions in the cloned fragments of mitochondrial (mt) dna from penicillium chrysogenum nrrl1951 (sekiguchi j., ohsaki, t., yamamoto, h., koichi, k. and shida, t. (1990) j. gen. microbiol. 136, 535-543) was determined and compared with those in aspergillus nidulans and neurospora crassa mitochondrial dnas. the p. chrysogenum mt 1-rrna gene has a 1678 bp intron which intervenes between a 2835 bp 5' exon and a 581 bp 3' exon, an ... | 1993 | 7680578 |
| an evaluation of the use of in vitro tubulin polymerisation, fungal and wheat assays to detect the activity of potential chemical aneugens. | the test chemicals included in the ec aneuploidy project were evaluated for their ability to induce aneuploidy or aneuploidy related endpoints in assays using in vitro tubulin polymerisation, fungi and wheat. the results obtained demonstrated considerable qualitative and quantitative differences between the responses of the assays to the 10 test chemicals. fungal assays failed to respond to the potent mammalian spindle poisons colchicine and vinblastine and only three chemicals were positive in ... | 1993 | 7683381 |
| design of an object-oriented database for reverse genetics. | we present the design of an object-oriented database system for reverse genetics applications. such a database will encapsulate not only the data in the genetic and physical maps, but also the methods used to create the maps as well as methods to link them to other databases, such as genbank, pir, and medline. the purpose of this database is to provide the fungal genetics community with an electronic tool for identifying the biochemical function of any dna fragment in the database--electronic re ... | 1993 | 7584341 |
| metabolism of folate glutamates in aspergillus nidulans. | 1993 | 7508173 | |
| a glucose-derepressed promoter for expression of heterologous products in the filamentous fungus aspergillus nidulans. | we describe a putative binding sequence (gcggggc) for the glucose-responsive repressor protein crea at two positions upstream of the transcription start site of the alcohol dehydrogenase i (alca) gene of aspergillus nidulans. to positively identify the putative binding sites as crea-specific, the gcggggc blocks were mutated at five internal nucleotide positions to gtactac and reintroduced into the wild type alca promoter driving expression of the endogenous alcohol dehydrogenase i gene. this cre ... | 1993 | 7763860 |
| cloning of the aspergillus niger gene encoding alpha-l-arabinofuranosidase a. | using l-arabitol as an inducer, simple induction conditions were established that resulted in high-level expression of alpha-l-arabinofuranosidase a by an aspergillus niger d-xylulose kinase mutant strain. these conditions were adapted to construct a cdna expression library from which an alpha-l-arabinofuranosidase a cdna clone was isolated using specific antiserum. the corresponding gene encoding alpha-l-arabinofuranosidase a (abfa) was isolated from a genomic library and cloned into a high cop ... | 1993 | 7764056 |
| development of a new transformant selection system for penicillium chrysogenum: isolation and characterization of the p. chrysogenum acetyl-coenzyme a synthetase gene (faca) and its use as a homologous selection marker. | a new transformation system for the filamentous fungus penicillium chrysogenum is described, based on the use of the homologous acetyl-coenzyme a synthetase (faca) gene as a selection marker. acetate-non-utilizing (fac-) strains of p. chrysogenum were obtained by positive selection for spontaneous resistance to fluoroacetate. among these fac mutants putative faca strains were selected for a loss of acetyl-coenzyme a (coa) synthetase activity. the faca gene, coding for the enzyme acetyl-coa synth ... | 1993 | 7765289 |
| evidence for a multi-allelic heterokaryon incompatibility (het) locus detected by hybridization among three heterokaryon-compatibility (h-c) groups of aspergillus nidulans. | a strain of heterokaryon-compatibility (h-c) group a was crossed sexually to strains of h-cb and h-cgl of aspergillus nidulans. a back-crossing programme established that there were seven hetero-allelic heterokaryon compatibility (het) genes controlling somatic incompatibility between strains of h-ca and h-cb. a similar back-crossing programme between strains of h-ca and h-cgl confirmed that these two groups differ at six het loci. previous work has shown that h-cb differs from h-cgl at two het ... | 1993 | 8314715 |
| spatial and temporal controls of the aspergillus brla developmental regulatory gene. | the aspergillus nidulans brla gene encodes a transcriptional regulator of central importance in controlling conidiophore development. i have determined the effects of mutations in other developmental regulatory genes on expression of a brla-lacz fusion gene. deletion of brla reduced beta-galactosidase levels by half and led to delocalization of enzyme accumulation. the meda26 and abaa2 developmental mutations led to overexpression of the fusion gene without altering spatial specificity. in contr ... | 1993 | 8316075 |
| choline: its role in the growth of filamentous fungi and the regulation of mycelial morphology. | choline is an essential metabolite for the growth of filamentous fungi. it occurs most notably as a component of the major membrane phospholipid, phosphatidyl choline (lecithin), and fulfills a major role in sulphate metabolism in the form of choline-o-sulphate in many species. choline is usually synthesised endogenously, but exogenous choline can also be taken up, either to compensate for metabolic deficiencies in choline-requiring mutants such as those of aspergillus nidulans and neurospora cr ... | 1993 | 8318261 |
| isolation and nucleotide sequence of the 5-aminolevulinate synthase gene from aspergillus nidulans. | the structural gene for 5-aminolevulinate (ala) synthase has been cloned and sequenced from the filamentous fungus aspergillus nidulans using an oligonucleotide probe based on a highly conserved-amino-acid sequence found in ala synthase genes of a wide range of species. the cloned gene, hema, has a 5' untranslated mrna of 92 nucleotides (nt) and one intron (64 nt). the deduced protein sequence (648 amino acids) shows 64% identity to the yeast ala synthase in the c-terminal region of 453 amino ac ... | 1993 | 8319309 |
| complement binding to aspergillus conidia correlates with pathogenicity. | complement has significant effects on the phagocytosis of aspergillus organisms. we examined the amount and type of complement component c3 bound to the resting conidia of 29 isolates from nine aspergillus species. the highly pathogenic species a. fumigatus and a. flavus bound fewer c3 molecules per unit of conidial surface area than did the less pathogenic species a. glaucus, a. nidulans, a. niger, a. ochraceus, a. terreus, a. versicolor, and a. wentii, as determined by quantitative flow cytome ... | 1993 | 8320488 |
| the isolation of mutagen-sensitive nuv mutants of aspergillus nidulans and their effects on mitotic recombination. | more than 200 mutants of aspergillus nidulans were isolated as hypersensitive to the monofunctional alkylating agent mnng and/or uv-irradiation (designated nuv mutants). of these, 23 were selected for further characterization. all were markedly hypersensitive to both mnng and the quasi-uv-mimetic mutagen 4-nqo. the hypersensitive phenotype of each mutant was shown to result from mutation of a single gene. the nuv mutants exhibited a diverse range of growth responses on solid media containing var ... | 1993 | 8325481 |
| either alpha-tubulin isogene product is sufficient for microtubule function during all stages of growth and differentiation in aspergillus nidulans. | the filamentous fungus aspergillus nidulans has two genes encoding alpha-tubulin, tuba and tubb, which are differentially required at distinct stages during the life cycle. the tuba gene is required during vegetative growth for mitosis and nuclear migration (b. r. oakley, c. e. oakley, and j. e. rinehart, mol. gen. genet. 208:135-144, 1987; p. doshi, c. a. bossie, j. h. doonan, g. s. may, and n. r. morris, mol. gen. genet. 225:129-141, 1991). the tubb gene is not required for any detectable aspe ... | 1993 | 8336695 |
| uvsi mutants defective in uv mutagenesis define a fourth epistatic group of uvs genes in aspergillus. | three uv-sensitive mutations of a. nidulans, uvsi, uvsj and uvsa, were tested for epistatic relationships with members of the previously established groups, here called the "uvsf", "uvsc", and "uvsb" groups. uvsi mutants are defective for spontaneous and induced reversion of certain point mutations and differ also for other properties from previously analyzed uvs types. they are very sensitive to the killing effects of uv-light and 4-nqo (4-nitro-quinoline-n-oxide) but not to mms (methylmethane ... | 1993 | 8358834 |
| the aspergillus niger carbon catabolite repressor encoding gene, crea. | in order to undertake a comparative analysis of carbon catabolite repression in two aspergillus species, the crea gene has been isolated from a. niger by cross hybridization, using the cloned a. nidulans gene. the a. niger gene has been shown to be functional in a. nidulans by heterologous complementation of the crea204 mutation of a. nidulans. overall, the genes show 90% sequence similarity (82% identity) at the amino acid (aa) level. there were some striking similarities between the aa sequenc ... | 1993 | 8359691 |
| characterization of the pyruvate kinase-encoding gene (pki1) of trichoderma reesei. | the pyruvate kinase-encoding gene (pki1) from trichoderma reesei was isolated by hybridization to the corresponding aspergillus nidulans pkia gene. the 1614-bp nucleotide (nt) sequence of the cloned gene codes for a 538-amino-acid protein. the coding sequence contains a single intron of 246 nt at a position identical to that of intron e in the a. nidulans gene. the pki protein shows extensive homology to the pkis of a. nidulans and a. niger (67%) and saccharomyces cerevisiae (59%). the 5' non-co ... | 1993 | 8359694 |
| the gamma-tubulin gene of the malaria parasite plasmodium falciparum. | by screening of cdna and genomic libraries of plasmodium falciparum with a dna probe derived from the cognate beta-tubulin gene, gene pf gamma tub has been identified that codes for gamma-tubulin, a newly discovered member of the tubulin superfamily that is indispensible for nuclear division and microtubule assembly [12]. gene pf gamma tub is not interrupted by introns and only present as a single-copy in the parasite genome. its encoded amino acid sequence (452 amino acids; m(r) 50,560) has a 6 ... | 1993 | 8366893 |
| the nia gene of fusarium oxysporum: isolation, sequence and development of a homologous transformation system. | the fusarium oxysporum gene nia, encoding nitrate reductase (nr), was isolated from a cosmid library by direct complementation of an f. oxysporum nia- mutant. the gene specifies a protein of 905 amino acids and contains a 57-bp intron. comparison of the deduced aa sequence with nr of other fungi revealed a high degree of similarity and conservation in the intron position. the cloned nia made it possible to develop the first homologous transformation system for this fungus. transformation frequen ... | 1993 | 8370541 |
| analysis of heterokaryon incompatibility between heterokaryon-compatibility (h-c) groups r and gl provides evidence that at least eight het loci control somatic incompatibility in aspergillus nidulans. | protoplast fusion was used to hybridize strains 99-6 (h-cr) and 7-141 (h-cgl) in the birmingham collection of aspergillus nidulans. a sparsely conidiating, brown-pigmented diploid strain (ma7) was isolated. inocula from ma7 were haploidized on medium containing benlate and a haploid progeny sample collected. heterokaryon compatibility testing of selected progeny strains assayed each linkage group in turn and established that h-cr and h-cgl were hetero-allelic for heterokaryon incompatibility (he ... | 1993 | 8371120 |
| mutation in the bimd gene of aspergillus nidulans confers a conditional mitotic block and sensitivity to dna damaging agents. | mutation in the bimd gene of aspergillus nidulans results in a mitotic block in anaphase characterized by a defective mitosis. mutation in bimd also confers, at temperatures permissive for the mitotic arrest phenotype, an increased sensitivity to dna damaging agents, including methyl methanesulfonate and ultraviolet light. in order to better understand the relationship between dna damage and mitotic progression, we cloned the bimd gene from aspergillus. a cosmid containing the bimd gene was iden ... | 1993 | 8375649 |
| quantitative structure-activity relationship models correctly predict the toxic and aneuploidizing properties of six halogenated methanes in aspergillus nidulans. | in a previous study, the relationships between the chemical structure and the ability of 35 chlorinated aliphatic hydrocarbons to induce aneuploidy and toxicity in aspergillus nidulans were analyzed. quantitative structure-activity relationships (qsar) were defined for each of the biological activities under study: arr (the dose able to block mitotic growth), d37 (the dose with 37% of survival) and lec (the lowest efficient concentration in aneuploidy induction). in this study, these qsar equati ... | 1993 | 8377647 |
| development of a transformation system for the filamentous, ml-236b (compactin)--producing fungus penicillium citrinum. | we present here the first report of a transformation system developed for the filamentous, ml-236b (compactin)-producing fungus penicillium citrinum. hygromycin b-resistant colonies were obtained after treatment of protoplasts with a vector containing an escherichia coli hygromycin b phosphotransferase gene fused to a 3-phosphoglycerate kinase promoter from aspergillus nidulans. the transformation rate was 194 transformants per microgram circular dna per 4 x 10(5) viable protoplasts under optimi ... | 1993 | 8381335 |
| the molecular biology of the pentafunctional arom protein. | 1993 | 8383607 | |
| overproduction of, and interaction within, bifunctional domains from the amino- and carboxy-termini of the pentafunctional arom protein of aspergillus nidulans. | the pentafunctional arom protein in aspergillus nidulans and other fungi catalyses five consecutive enzymatic steps leading to the production of 5-enolpyruvylshikimate 3-phosphate (epsp) in the shikimate pathway. the arom protein has five separate enzymatic domains that have previously been shown to display a range of abilities to fold and function in isolation as monofunctional enzymes. in this communication, we report (1) the stable overproduction of a bifunctional protein containing the 3-deh ... | 1993 | 8393515 |
| ph regulation is a major determinant in expression of a fungal penicillin biosynthetic gene. | transcription of the ipna gene encoding isopenicillin n synthetase, an enzyme of secondary metabolism, is under the control of the ph regulatory system in the fungus aspergillus nidulans. external alkaline ph or mutations in pacc, the wide domain regulatory gene which mediates ph regulation, override carbon regulation of ipna transcript levels, resulting in elevation of the levels of this message in sucrose broth. strains carrying these mutations, which mimic growth at alkaline ph, produce highe ... | 1993 | 8404862 |
| purification and molecular properties of an alpha-galactosidase synthesized and secreted by aspergillus nidulans. | alpha-galactosidases from mycelial extract and culture filtrate of aspergillus nidulans have been purified to homogeneity and utilised to obtain polyclonal antibodies anti-alpha-galactosidase. the enzymatic characteristics and the cross reactivity of the antibodies suggest that alpha-galactosidases isolated from the two sources were the same enzyme. thus, a. nidulans synthesized and secreted only one enzymatic form of alpha-galactosidase which is a multimeric enzyme of 370 kda composed of four m ... | 1993 | 8405947 |
| biochemical characterization and molecular genetics of nine mutants of penicillium chrysogenum impaired in penicillin biosynthesis. | nine mutants of penicillium chrysogenum (npe1 to npe8 and npe10) impaired in penicillin biosynthesis were screened after nitrosoguanidine mutation. mutants npe1, npe4, npe5, npe6, npe7, npe8, and npe10 failed to synthesize significant levels of penicillin, whereas strains npe2 and npe3 synthesized about 20% of the penicillin level produced by the parental strain. mutants npe5 and npe10 did not show alpha-aminoadipylcysteinyl-valine (acv) synthetase activity in vitro and did not form acv in vivo. ... | 1993 | 8416976 |
| functional redundancy in mitotic force generation. | 1993 | 8416980 | |
| suppression of the bimc4 mitotic spindle defect by deletion of klpa, a gene encoding a kar3-related kinesin-like protein in aspergillus nidulans. | to investigate the relationship between structure and function of kinesin-like proteins, we have identified by polymerase chain reaction (pcr) a new kinesin-like protein in the filamentous fungus aspergillus nidulans, which we have designated klpa. dna sequence analysis showed that the predicted klpa protein contains a cooh terminal kinesin-like motor domain. despite the structural similarity of klpa to the kar3 and ncd kinesin-like proteins of saccharomyces cerevisiae and drosophila melanogaste ... | 1993 | 8416986 |
| identification of aspergillus brla response elements (bres) by genetic selection in yeast. | the brla gene of aspergillus nidulans plays a central role in controlling conidiophore development. to test the hypothesis that brla encodes a transcriptional regulator and to identify sites of interaction for the brla polypeptide, we expressed brla in saccharomyces cerevisiae (yeast) strains containing aspergillus dna sequences inserted upstream of a minimal yeast promoter fused to the escherichia coli lacz gene. initially, a dna fragment from the promoter region of the developmentally regulate ... | 1993 | 8417986 |
| genetic maps of eight linkage groups of aspergillus niger based on mitotic mapping. | this paper provides a genetic map of aspergillus niger. at present 84 markers have been assigned to eight linkage groups. the chromosomal location of 60 markers is presented in this paper. the allocation of markers is based on recombination due to mitotic crossing over. various methods for selection and analysis of homozygous recombinants were applied, using colour, auxotrophic and resistance markers. in addition, transformants carrying the heterologous aspergillus nidulans gene coding for aceta ... | 1993 | 8428383 |
| disruption of the 3' phosphoglycerate kinase (pgk) gene in aspergillus nidulans. | we report the isolation of a pgk- mutant strain of aspergillus nidulans by means of a gene disruption strategy, and demonstrate that the pgk gene is located on chromosome viii. the pgk- mutant conidiates poorly, will only grow on media supplemented with both a glycolytic and a gluconeogenic carbon source, and is inhibited by hexoses. | 1993 | 8431952 |
| cloning and sequencing of the 3-phosphoglycerate kinase (pgk) gene from penicillium citrinum and its application to heterologous gene expression. | the gene coding for 3-phosphoglycerate kinase (pgk) in ml-236b (compactin)-producing penicillium citrinum was isolated from the recombinant phage lambda library using the corresponding aspergillus nidulans pgk gene as a probe. the p. citrinum pgk gene has an open reading frame of 1,254 bp, encoding a protein of 417 amino acids with a predicted molecular weight of 44,079 daltons. the position of the two introns, 59 and 60 bp respectively, was deduced from an homology comparison with the sequence ... | 1993 | 8431954 |
| bima, a tpr-containing protein required for mitosis, localizes to the spindle pole body in aspergillus nidulans. | the aspergillus nidulans bima gene is required for mitosis. loss of function mutations in bima cause cells to arrest growth with condensed chromatin and a short, metaphaselike mitotic spindle. bima is a member of a gene family defined by a repeated motif called the tetratrico peptide repeat (tpr), which is found in genes from bacteria, yeast and insects. several yeast tpr genes are also required for mitosis, including saccharomyces cerevisiae cdc27 and schizosaccharomyces pombe nuc2+, which appe ... | 1993 | 8432735 |
| c-terminal truncation of the transcriptional activator encoded by area in aspergillus nidulans results in both loss-of-function and gain-of-function phenotypes. | mutations truncating as many as 143 c-terminal residues from the transcriptional activator encoded by the area gene, mediating nitrogen metabolite repression in aspergillus nidulans, do not significantly reduce the ability of the area product to activate expression of most genes under area control. such mutations can even have a gain-of-function, derepressed phenotype, consistent with a critical role for this region in modulating the activity of the area protein. however, expression of a few gen ... | 1993 | 8437521 |
| operator derepressed mutations in the proline utilisation gene cluster of aspergillus nidulans. | the proline utilisation gene cluster of aspergillus nidulans can be repressed efficiently only when both repressing nitrogen and repressing carbon sources are present. we show that two cis-acting mutations in this cluster permit the efficient transcription of the prnb gene under repressing conditions, resulting in direct or indirect derepression of two other transcripts of the pathway. these mutations are transitions that define a 5'gagacccc3' sequence. similar sequences are found upstream of ot ... | 1993 | 8437566 |
| cloning of two isozymes of trichoderma koningii glyceraldehyde-3-phosphate dehydrogenase with different sensitivity to koningic acid. | koningic acid inhibits glyceraldehyde-3-phosphate dehydrogenase (gapdh) by binding to the sh group in the active center. the fungus trichoderma koningii, the producer of koningic acid, contains two gapdh isozymes (gapdhs i and ii). gapdh i is inhibited 50% by 1.1.10(-3) m koningic acid, while gapdh ii is inhibited 50% at 6.8 x 10(-6) m. cdnas of the two isozymes were cloned from t. koningii and their nucleotide sequences were determined. the sequence of coding region and codon usage in both clon ... | 1993 | 8439569 |
| molecular cloning and analysis of abundant and stage-specific mrnas from puccinia graminis. | to characterize highly expressed mrnas from germinated urediniospores of puccinia graminis f. sp. tritici, we isolated 68 cdna clones of abundant mrna species belonging to at least six homology groups. the two most abundant homology groups, hg1 and hg2, contained 54 of the 68 cdna clones and accounted for 2.4 and 0.6% of the poly(a)+ rna in germinated urediniospores, respectively. by sampling different developmental stages of the uredinial cycle, we showed that the uam transcript, corresponding ... | 1993 | 8439672 |
| the mitochondrial genome of the entomopathogenic fungus beauveria bassiana: analysis of the ribosomal rna region. | the 28.5-kbp mitochondrial (mt) genome from the entomopathogenic fungus beauveria bassiana was studied using restriction enzyme analysis, gene probe hybridization, and dna sequence comparisons. a detailed restriction enzyme map allowed cloning of the entire genome into a number of segments. hybridization of heterologous gene probes to the mtdna resulted in the identification of the large ribosomal rna (lrrna) and small ribosomal rna (srrna) genes. gene probes derived from several yeasts and fung ... | 1993 | 8439871 |
| cerato-ulmin, a toxin involved in dutch elm disease, is a fungal hydrophobin. | 1993 | 8453298 | |
| aspergillus nidulans nuclear proteins bind to a ccaat element and the adjacent upstream sequence in the promoter region of the starch-inducible taka-amylase a gene. | aspergillus nidulans was used as an intermediate host to investigate the regulation of the taka-amylase a (taa) gene from aspergillus oryzae. the induction of taa by starch was confirmed to be regulated at the transcriptional level by analyzing the transcripts specific for taa synthesized in vitro in nuclei from starch- and glucose-grown cells. a 55 bp dna fragment containing a consensus ccaat sequence from the promoter region of the taa gene was shown to confer starch inducibility on the gene. ... | 1993 | 8455560 |
| evolutionary conservation of excision repair in schizosaccharomyces pombe: evidence for a family of sequences related to the saccharomyces cerevisiae rad2 gene. | cells mutated at the rad13 locus in the fission yeast, schizosaccharomyces pombe are deficient in excision-repair of uv damage. we have cloned the s.pombe rad13 gene by its ability to complement the uv sensitivity of a rad13 mutant. the gene is not essential for cell proliferation. sequence analysis of the cloned gene revealed an open reading-frame of 1113 amino acids with structural homology to the rad2 gene of the distantly related saccharomyces cerevisiae. the sequence similarity is confined ... | 1993 | 8464724 |
| carnitine acetyltransferase is absent from acuj mutants of aspergillus nidulans. | the acuj mutant of aspergillus nidulans has been shown to lack carnitine acetyltransferase (cat) activity when grown under conditions where this activity is readily detectable in wild-type strains. revertants selected for growth on acetate recover cat activity and the ability to grow on long-chain fatty acids. when growing on carbon sources such as sucrose, cytosolic acetyl coenzyme a was generated by adenosine triphosphate (atp):citrate lyase. | 1993 | 8472927 |
| properties and regulation of the cell cycle-specific nima protein kinase of aspergillus nidulans. | nima is the protein product of the nima gene of the filamentous fungus aspergillus nidulans, required for progression of cells from g2 into mitosis. the protein kinase activity of nima, assayed by phosphorylation of beta-casein, varies during the nuclear division cycle, reaching a maximum in late g2 and m. to investigate the biochemical properties of this cell cycle-regulated protein kinase, we have expressed nima cdna that encodes full-length nima in escherichia coli as a fusion product with gl ... | 1993 | 8473320 |