Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| [chronology of a pandemic: the new influenza a (h1n1) in bogota, 2009-2010]. | describing the behaviour of the a h1n1/09 virus related to time, age and gender in patients having suspected infection, medical health care and laboratory diagnosis. | 2011 | 22031000 |
| viral-bacterial interactions and risk of acute otitis media complicating upper respiratory tract infection. | acute otitis media (aom) is a common complication of upper respiratory tract infection whose pathogenesis involves both viruses and bacteria. we examined risks of acute otitis media associated with specific combinations of respiratory viruses and acute otitis media bacterial pathogens. data were from a prospective study of children ages 6 to 36 months and included viral and bacterial culture and quantitative pcr for respiratory syncytial virus (rsv), human bocavirus, and human metapneumovirus. r ... | 2011 | 21900518 |
| aetiology of upper respiratory tract infections in children in arak city: a community based study. | viruses are frequent causes of upper respiratory tract infections in children. we investigated the viral aetiology of community-acquired upper respiratory tract infections (uris) in young children treated as outpatients in community settings.during november 2008, nasal swab specimens were taken from children with recent onset of upper respiratory tract infections. the patients attended day care or primary schools; the specimens were randomly obtained by pediatricians from schools and childcare i ... | 2011 | 22207287 |
| comparison of differing cytopathic effects in human airway epithelium of parainfluenza virus 5 (w3a), parainfluenza virus type 3, and respiratory syncytial virus. | parainfluenza virus 5 (piv5) infects a wide range of animals including dogs, pigs, cats, and humans; however, its association with disease in humans remains controversial. in contrast to parainfluenza virus 3 (piv3) or respiratory syncytial virus (rsv), piv5 is remarkably non-cytopathic in monolayer cultures of immortalized epithelial cells. to compare the cytopathology produced by these viruses in a relevant human tissue, we infected an in vitro model of human ciliated airway epithelium and mea ... | 2011 | 21986028 |
| nanobodies® specific for respiratory syncytial virus fusion protein protect against infection by inhibition of fusion. | despite the medical importance of respiratory syncytial virus (rsv) infections, there is no vaccine or therapeutic agent available. prophylactic administration of palivizumab, a humanized monoclonal rsv fusion (f) protein-specific antibody, can protect high-risk children. previously, we have demonstrated that rsv can be neutralized by picomolar concentrations of a camelid immunoglobulin single-variable domain that binds the rsv protein f (f-vhhb nanobodies). here, we investigated the mechanism b ... | 2011 | 21998474 |
| human metapneumovirus. | human metapneumovirus (hmpv) is a common pathogen that can cause both upper and lower respiratory tract infections, particularly in children, elderly adults, and immunocompromised hosts. since its initial identification in 2001, hmpv has been isolated from individuals with acute respiratory tract infections (artis) in virtually every continent. serological studies indicate that it has caused human infection since 1958 or earlier. the epidemiology and clinical manifestations of hmpv are similar t ... | 2011 | 21858749 |
| effect of reduction in household air pollution on childhood pneumonia in guatemala (respire): a randomised controlled trial. | pneumonia causes more child deaths than does any other disease. observational studies have indicated that smoke from household solid fuel is a significant risk factor that affects about half the world's children. we investigated whether an intervention to lower indoor wood smoke emissions would reduce pneumonia in children. | 2011 | 22078686 |
| respiratory syncytial virus glycoprotein g interacts with dc-sign and l-sign to activate erk1 and erk2. | respiratory syncytial virus (rsv) interaction with epithelial and dendritic cells (dcs) is known to require divalent cations, suggesting involvement of c-type lectins. rsv infection and maturation of primary human dcs are reduced in a dose-dependent manner by edta. therefore, we asked whether rsv infection involves dc-sign (cd209) or its isoform l-sign (cd299) (dc-sign/r). using surface plasmon resonance analysis, we demonstrated that the attachment g glycoprotein of rsv binds both dc- and l-sig ... | 2011 | 22090124 |
| association of herd bhv-1 seroprevalence with respiratory disease in youngstock in estonian dairy cattle. | the associations between herd bovine herpesvirus 1 (bhv-1) seroprevalence, along with other infectious and farm management factors with bovine respiratory disease (brd) in dairy calves and heifers, were investigated. serum samples from 103 dairy cattle herds were analyzed for antibodies against bhv-1, bovine respiratory syncytial virus (brsv), bovine viral diarrhea virus (bvdv), and mycoplasma bovis (m. bovis). a questionnaire was used to record herd management practices. a high occurrence of re ... | 2011 | 22100246 |
| first influenza season after the 2009 pandemic influenza: characteristics of intensive care unit admissions in adults and children in vall d'hebron hospital. | clin microbiol infect abstract: to assess potential differences in epidemiology and management of patients admitted with influenza infection in the intensive care unit (icu) during the first post-pandemic influenza period. observational prospective study comparing september 2009-january 2010 with september 2010-january 2011. variables captured: demographics, co-morbidities, physiological parameters, outcomes and management. analysis was performed using spss v. 13.0; significance was set at p 0.5 ... | 2011 | 21851487 |
| resveratrol-mediated gamma interferon reduction prevents airway inflammation and airway hyperresponsiveness in respiratory syncytial virus-infected immunocompromised mice. | respiratory syncytial virus (rsv) is the most important cause of severe, lower respiratory tract infections in infants, and rsv infections have been associated with chronic wheezing and asthma during childhood. however, the mechanism of rsv-induced airway inflammation and airway hyperresponsiveness (ahr) is poorly understood. furthermore, there are presently neither effective vaccines nor drugs available for the prevention or treatment of rsv infections. in this study, we investigated the effect ... | 2011 | 21937650 |
| down syndrome and hospitalizations due to respiratory syncytial virus: a population-based study. | objective: to assess the risk estimates for respiratory syncytial virus (rsv) hospitalization in children with down syndrome (ds) and the clinical features and severity of rsv lower respiratory tract infection (lrti) in hospitalized children. study design: statewide hospitalization data for children with ds for 1995 through 2006 from the colorado health and hospital association database were combined with birth data from the colorado department of public health and environment to obtain populati ... | 2011 | 22177993 |
| immunoprotectivity of hla-a2 ctl peptides derived from respiratory syncytial virus fusion protein in hla-a2 transgenic mouse. | identification of hla-restricted cd8+ t cell epitopes is important to study rsv-induced immunity and illness. we algorithmically analyzed the sequence of the fusion protein (f) of respiratory syncytial virus (rsv) and generated synthetic peptides that can potentially bind to hla-a*0201. four out of the twenty-five 9-mer peptides tested: peptides 3 (f33-41), 13 (f214-222), 14 (f273-281), and 23 (f559-567), were found to bind to hla-a*0201 with moderate to high affinity and were capable of inducin ... | 2011 | 21980478 |
| Cholesterol-rich Microdomains as Docking Platforms for Respiratory Syncytial Virus in Normal Human Bronchial Epithelial Cells. | Respiratory syncytial virus (RSV) is one of the major causes of respiratory infections in children and it is the main pathogen causing bronchiolitis in infants. The binding and entry mechanism by which RSV infects respiratory epithelial cells has not yet been determined. In this study, the earliest stages of RSV infection in normal human bronchial epithelial cells were probed by tracking virions with fluorescent lipophilic dyes in their membranes. Virions colocalized with cholesterol-containing ... | 2011 | 22090136 |
| identification of potential human respiratory syncytial virus and metapneumovirus t cell epitopes using computational prediction and mhc binding assays. | human respiratory syncytial virus (rsv) and human metapneumovirus (mpv) are two of the most common causes of serious viral lower respiratory tract illness in humans. cd8+ t cells have been shown to be important in animal models and human clinical studies for the clearance of viral infection, and they may contribute in part to protection against severe disease during reinfections. precise enumeration and accurate phenotyping of rsv- or mpv-specific cd8+ t cells in humans is currently limited by t ... | 2011 | 21854782 |
| Requirements for Human Respiratory Syncytial Virus Glycoproteins in Assembly and Egress from Infected Cells. | Human respiratory syncytial virus (HRSV) is an enveloped RNA virus that assembles and buds from the plasma membrane of infected cells. The ribonucleoprotein complex (RNP) must associate with the viral matrix protein and glycoproteins to form newly infectious particles prior to budding. The viral proteins involved in HRSV assembly and egress are mostly unexplored. We investigated whether the glycoproteins of HRSV were involved in the late stages of viral replication by utilizing recombinant virus ... | 2011 | 21931576 |
| evaluation of type-specific real-time pcr assays using the lightcycler and j.b.a.i.d.s. for detection of adenoviruses in species hadv-c. | sporadically, hadvs from species hadv-c are detected in acute respiratory disease outbreaks. to rapidly type these viruses, we designed real-time pcr assays that detect and discriminate between adenovirus types hadv-c1, -c2, -c5, and -c6. sixteen clinical isolates from the california department of public health were used to validate the new assays. type-specific taqman real-time pcr assays were designed and used independently to successfully identify 16 representative specimens. the lower limit ... | 2011 | 22046386 |
| Respiratory support by neurally adjusted ventilatory assist (NAVA) in severe RSV-related bronchiolitis: a case series report. | Neurally adjusted ventilatory assist (NAVA) is a new mode of mechanical ventilation controlled by diaphragmatic electrical signals. The electrical signals allow synchronization of ventilation to spontaneous breathing efforts of a child, as well as permitting pressure assistance proportional to the electrical signal. NAVA provides equally fine synchronization of respiratory support and pressure assistance varying with the needs of the child. NAVA has mainly been studied in children who underwent ... | 2011 | 22014152 |
| The first two nucleotides of the respiratory syncytial virus antigenome RNA replication product can be selected independently of the promoter terminus. | There is limited knowledge regarding how the RNA-dependent RNA polymerases of the nonsegmented negative-strand RNA viruses initiate genome replication. In a previous study of respiratory syncytial virus (RSV) RNA replication, we found evidence that the polymerase could select the 5'-ATP residue of the genome RNA independently of the 3' nucleotide of the template. To investigate if a similar mechanism is used during antigenome synthesis, a study of initiation from the RSV leader (Le) promoter was ... | 2011 | 21878549 |
| The post-infection outcomes of influenza and acute respiratory infection in patients above 50 years of age in Japan: an observational study. | Please cite this paper as: Ikematsu et al. (2011) The post-infection outcomes of influenza and acute respiratory infection in patients above 50 years of age in Japan: an observational study. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2011.00296.x. Objectives Influenza can be a serious illness, especially for older people, and reducing the impact of influenza in elderly is important. The objective of this study was to estimate the prevalence and postinfection outcomes of in ... | 2011 | 21985038 |
| Silver nanoparticles as potential antiviral agents. | Virus infections pose significant global health challenges, especially in view of the fact that the emergence of resistant viral strains and the adverse side effects associated with prolonged use continue to slow down the application of effective antiviral therapies. This makes imperative the need for the development of safe and potent alternatives to conventional antiviral drugs. In the present scenario, nanoscale materials have emerged as novel antiviral agents for the possibilities offered by ... | 2011 | 22024958 |
| il-17 boosts proinflammatory outcome of antiviral response in human cells. | excessive inflammation during bacterial and viral infections is destructive to the host and involves elevated production of proinflammatory cytokines. it is especially deleterious in organs with space constraints such as lung and the cns. indeed, a number of viruses that infect lungs, such as avian influenza virus, sars-associated coronavirus, and respiratory syncytial virus, elicit a very high level of proinflammatory cytokines; however, it is unclear what triggers their production. in this stu ... | 2011 | 21964025 |
| respiratory syncytial virus vaccine development. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract viral disease in infants and young children. presently, there are no explicit recommendations for rsv treatment apart from supportive care. the virus is therefore responsible for an estimated 160,000 deaths per year worldwide. despite half a century of dedicated research, there remains no licensed vaccine product. herein are described past and current efforts to harness innate and adaptive immune potentials to comb ... | 2011 | 21988307 |
| Severe acute respiratory syndrome coronavirus envelope protein regulates cell stress response and apoptosis. | Severe acute respiratory syndrome virus (SARS-CoV) that lacks the envelope (E) gene (rSARS-CoV-?E) is attenuated in vivo. To identify factors that contribute to rSARS-CoV-?E attenuation, gene expression in cells infected by SARS-CoV with or without E gene was compared. Twenty-five stress response genes were preferentially upregulated during infection in the absence of the E gene. In addition, genes involved in signal transduction, transcription, cell metabolism, immunoregulation, inflammation, a ... | 2011 | 22028656 |
| Viral aetiology of influenza-like illness in Belgium during the influenza A(H1N1)2009 pandemic. | The purpose of this investigation was to determine the proportion of influenza-like illness (ILI) attributable to specific viruses during the influenza A(H1N1)2009 pandemic and to describe the demographic and clinical characteristics of ILI due to respiratory viruses in Belgium. Nasopharyngeal swabs were collected from ILI patients by general practitioners (GPs) and paediatricians (PediSurv) and analysed for viruses. Of 139 samples collected from children <5 years of age by PediSurv, 86 were pos ... | 2011 | 21901635 |
| twenty-first century vaccines. | in the twentieth century, vaccination has been possibly the greatest revolution in health. together with hygiene and antibiotics, vaccination led to the elimination of many childhood infectious diseases and contributed to the increase in disability-free life expectancy that in western societies rose from 50 to 78-85 years (crimmins, e. m. & finch, c. e. 2006 proc. natl acad. sci. usa 103, 498-503; kirkwood, t. b. 2008 nat. med 10, 1177-1185). in the twenty-first century, vaccination will be expe ... | 2011 | 21893537 |
| The respiratory syncytial virus transcription antiterminator M(2-1) is a highly stable, zinc binding tetramer with strong pH-dependent dissociation and a monomeric unfolding intermediate. | The human respiratory syncytial virus M(2-1) transcription antiterminator is an essential elongation factor required by the RNA polymerase for effective transcription beyond the first two nonstructural genes. Its exclusive presence in pneumovirus among all paramyxovirus suggests a unique function within this small genus. With the aim of understanding its biochemical properties, we investigated this a-helical tetramer by making use of a biophysical approach. We found that the tetramer hydrodynami ... | 2011 | 21877705 |
| CT of viral lower respiratory tract infections in adults: comparison among viral organisms and between viral and bacterial infections. | We retrospectively compared the CT findings of consecutive viral and bacterial lower respiratory tract infections (LRTIs) to determine their imaging appearance and any definable differences among the causative viruses and between the viral and bacterial infections. | 2011 | 22021500 |
| Evidence for a causal relationship between respiratory syncytial virus infection and asthma. | Respiratory syncytial virus (RSV) infects all children early in life, is the most common cause of infant lower respiratory tract infections, and causes disease exacerbations in children with asthma. Episodes of lower respiratory tract infection in early life are associated with asthma development. Whether RSV infection early in life directly causes asthma or simply identifies infants who are genetically predisposed to develop subsequent wheezing is debatable. Recent studies suggest that these tw ... | 2011 | 21905783 |
| dp2 (crth2) antagonism reduces ocular inflammation induced by allergen challenge and respiratory syncytial virus. | background: allergic conjunctivitis is characterized by itchy, watery and swollen eyes which occur in response to exposure to seasonal or environmental allergens. the early phase reaction of allergic conjunctivitis is primarily mediated by mast cell degranulation while the late phase reaction is driven by th2 cells and eosinophils. prostaglandin d(2) (pgd(2)), released from mast cells, is present in allergic conjunctival tears and may elicit classical allergic responses via interaction with the ... | 2011 | 22042170 |
| Central role of dendritic cells in shaping the adaptive immune response during respiratory syncytial virus infection. | Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract disease in young children. Premature infants, immunocompromised individuals and the elderly exhibit the highest risk for the development of severe RSV-induced disease. Murine studies demonstrate that CD8 T cells mediate RSV clearance from the lungs. Murine studies also indicate that the host immune response contributes to RSV-induced morbidity as T-cell depletion prevents the development of disease despite sustaine ... | 2011 | 21887154 |
| progress in the development of human parainfluenza virus vaccines. | in children under 5 years of age, human parainfluenza viruses (hpivs) as a group are the second most common etiology of acute respiratory illness leading to hospitalization, surpassed only by respiratory syncytial virus but ahead of influenza viruses. using reverse genetics systems for hpiv serotypes 1, 2 and 3 (hpiv1, 2 and 3), several live-attenuated hpivs have been generated and evaluated as intranasal vaccines in adults and in children. two vaccines against hpiv3 were found to be well tolera ... | 2011 | 21859271 |
| [aetiology of bronchiolitis in hospitalised children in south-east spain.] | background: bronchiolitis is the most common respiratory disease in children under 2 years-old and a major cause of hospitalization in young children, especially during the winter. objectives: to determine the prevalence and etiology of bronchiolitis in south-east of spain. study design: a prospective study was conducted during the bronchiolitis season (december-april). children below 18 months-old admitted to the hospital for a first bronchiolitis episode were included. nasopharyngeal aspirates ... | 2011 | 22104022 |
| effect of injectable trace minerals on the humoral immune response to multivalent vaccine administration in beef calves. | the objective of this experiment was to investigate the effects of injectable trace minerals on humoral responses of calves receiving a viral vaccination. beef steer calves (n = 99; average bw = 316 ± 4.2 kg), seronegative for bovine herpesvirus-1 (bhv-1) and bovine viral diarrhea virus, genotypes 1 and 2 (bvdv-1 and bvdv-2) were sourced from 2 locations. all calves, except 15 non-vaccinated (sentinel) calves, received a single dose of a multivalent modified live vaccine (titanium 5; agrilabs, s ... | 2011 | 22178855 |
| viral epidemiology of acute exacerbations of chronic obstructive pulmonary disease. | the role of viruses in acute exacerbations of chronic obstructive pulmonary disease (aecopd) needs further elucidation. the aim of the present study was to evaluate the molecular epidemiology of viral pathogens in aecopd. patients presenting to the emergency room with aecopd needing hospitalization were recruited. oropharyngeal and sputum samples were collected in order to perform microarrays-based viral testing for the detection of respiratory viruses. a total of 200 (100%) patients were analyz ... | 2011 | 21983132 |
| seasonal variations in clostridium difficile infections are associated with influenza and respiratory syncytial virus activity independently of antibiotic prescriptions: a time series analysis in quebec, canada. | seasonal variations in clostridium difficile-associated diarrhea (cdad), with a higher incidence occurring during winter months, have been reported. although winter epidemics of respiratory viruses may be temporally associated with an increase in cdad morbidity, we hypothesized that this association is mainly due to increased antibiotic use for respiratory infections. the objective of this study was to evaluate the effect of the two most frequent respiratory viruses (influenza virus and respirat ... | 2011 | 22106208 |
| bug breakfast in the bulletin: respiratory syncytial virus. | 2011 | 21982261 | |
| human rhinovirus species associated with hospitalizations for acute respiratory illness in young us children. | the contribution of human rhinovirus (hrv) to severe acute respiratory illness (ari) is unclear. | 2011 | 22013207 |
| autophagy-mediated dendritic cell activation is essential for innate cytokine production and apc function with respiratory syncytial virus responses. | the regulation of innate immune responses during viral infection is a crucial step to promote antiviral reactions. recent studies have drawn attention to a strong relationship of pathogen-associated molecular pattern recognition with autophagy for activation of apc function. our initial observations indicated that autophagosomes formed in response to respiratory syncytial virus (rsv) infection of dendritic cells (dc). to further investigate whether rsv-induced dc activation and innate cytokine p ... | 2011 | 21911604 |
| a respiratory syncytial virus isolate enables the testing of virucidal products. | the respiratory syncytial virus (rsv) is known as a major cause of respiratory infections and nosocomial diseases. testing this virus is rather difficult due to the problems encountered in producing it at a high titer without using any purification method. a rsv isolate which replicates to high level on a hep-2 cell line with an infectious titer of at least 10(7)tcid(50)ml(-1) in culture supernatant fluids has been identified. thanks to this isolate, the virucidal effects of two products, a hand ... | 2011 | 22079427 |
| antiviral drugs for viruses other than human immunodeficiency virus. | most viral diseases, with the exception of those caused by human immunodeficiency virus, are self-limited illnesses that do not require specific antiviral therapy. the currently available antiviral drugs target 3 main groups of viruses: herpes, hepatitis, and influenza viruses. with the exception of the antisense molecule fomivirsen, all antiherpes drugs inhibit viral replication by serving as competitive substrates for viral dna polymerase. drugs for the treatment of influenza inhibit the ion c ... | 2011 | 21964179 |
| high frequency of human bocavirus 1 dna in infants and adults with lower acute respiratory infection. | human bocavirus (hbov) is a parvovirus with a possible etiological role in respiratory disease currently under investigation. we detected hbov1 in children and adults hospitalized with acute disease of the lower respiratory tract. hbov genome was detected by pcr in nasopharyngeal swabs collected from 75 patients aged 0-89 years old during 2010. hbov was found in 17/75 (22.7%) patients, 64.7% of them infants younger than 1 year old and 29.4% adults older than 30 years (the bimodal age distribu ... | 2011 | 22116985 |
| [a guinea pig model of respiratory syncytial virus infection for cough and its neurogenic inflammatory mechanism]. | to establish a guinea pig model of acute and postinfectious cough caused by respiratory syncytial virus (rsv) infection and investigate the role of neurogenic inflammation in its pathogenesis. | 2011 | 21914323 |
| applications of pcr (real-time and masstag) and enzyme-linked immunosorbent assay in diagnosis of respiratory infections and diarrheal illness among deployed u.s. military personnel during exercise balikatan 2009, philippines. | laboratory-based surveillance for diarrheal and respiratory illness was conducted at the 2009 republic of the philippines-united states balikatan exercise to determine the presence of specific pathogens endemic in the locations where the military exercises were conducted. ten stool and 6 respiratory specimens were obtained from individuals meeting case definitions for diarrhea or respiratory illness. stool specimens were frozen in dry ice and remotely tested using enzyme-linked immunosorbent ass ... | 2011 | 22128641 |
| low neonatal toll-like receptor 4-mediated interleukin-10 production is associated with subsequent atopic dermatitis. | atopic dermatitis (ad) and respiratory syncytial virus lower respiratory tract infection (rsv lrti) are common diseases during early life. impaired th1-cell polarizing toll-like receptor (tlr) responses play an important role in the pathogenesis of both diseases. neonatal tlr-mediated production of th1-type cytokines is decreased at birth, but rapidly increases during the first month of life. | 2011 | 22092594 |
| respiratory syncytial virus load, viral dynamics, and disease severity in previously healthy naturally infected children. | respiratory syncytial virus (rsv) disease severity was thought to be a result of host immunopathology but alternatively may be driven by high-level viral replication. the relationships between rsv load, viral clearance dynamics, and disease severity have not been carefully evaluated. | 2011 | 21881113 |
| in vitro inhibition of influenza virus infection by a crude extract from isatis indigotica root resulting in the prevention of viral attachment. | isatis indigotica root (iir) has been widely used as a chinese medicinal herb to treat regular seasonal influenza over the long history of traditional chinese medicinal practice. however, its inhibitory activities against influenza virus infections along with the associated mechanisms have not been investigated comprehensively. in this study, the chemical nature, mode of action and in vitro anti-influenza activities of a crude extract ( ... | 2011 | 22179315 |
| performance on a multipathogen external quality assessment (eqa) panel by different mono- and multiplex nucleic acid amplification tests. | an external quality assessment (eqa) panel consisting of a total of 48 samples in broncho alveolar lavage (bal) or transport medium was prepared in collaboration with quality control for molecular diagnostics (qcmd) (www.qcmd.org). the panel was used to assess the proficiency of the 3 laboratories that would be responsible to examine the 6000 samples to be collected in the grace network of excellence (www.grace-lrti.org). the main objective was to decide on the best performing testing approach f ... | 2011 | 22170925 |
| severity of viral coinfection in hospitalized infants with respiratory syncytial virus infection. | 2011 | 22011744 | |
| airway ifn-γ production during rsv bronchiolitis is associated with eosinophilic inflammation. | study objective: this study was designed to investigate the possible role of ifn-γ in eosinophil degranulation that occurs during respiratory syncytial virus (rsv) bronchiolitis. methods: sixty-seven infants, 2-24 months old and hospitalized with their first episode of acute rsv bronchiolitis, were selected for this study. eosinophil-active cytokine and chemokine profiles in nasal lavage supernatants taken within ... | 2011 | 22160185 |
| progress in understanding and controlling respiratory syncytial virus: still crazy after all these years. | human respiratory syncytial virus (rsv) is a ubiquitous pathogen that infects everyone worldwide early in life and is a leading cause of severe lower respiratory tract disease in the pediatric population as well as in the elderly and in profoundly immunosuppressed individuals. rsv is an enveloped, nonsegmented negative-sense rna virus that is classified in family paramyxoviridae and is one of its more complex members. although the replicative cycle of rsv follows the general pattern of the param ... | 2011 | 21963675 |
| use of data linkage to investigate the aetiology of acute lower respiratory infection hospitalisations in children. | aim: to document the aetiology of acute lower respiratory infection (alri) hospitalisations in western australian children by linking population-based laboratory data with hospital morbidity data. methods: data from all alri hospitalisations and laboratory records related to respiratory pathogens between 2000 and 2005 were extracted and linked through a population-based record linkage system. the proportion of specimens that were positive for each respiratory viral or bacterial pathogen was do ... | 2011 | 22077532 |
| polyclonal immunoglobulins and hyperimmune globulins in prevention and management of infectious diseases. | immunoglobulin therapy has a rich history of use in preventing and treating infectious diseases; however, clinical data on the efficacy of immunoglobulin is lacking for many infectious diseases. immunoglobulin therapy is routinely used in postexposure prophylaxis for bacterial infections, including tetanus, botulism, and diphtheria, and viral infections, including hepatitis a and b and varicella. immunoglobulin therapy has also been used in many severe and life-threatening infections where treat ... | 2011 | 22054755 |
| th17 cytokines are critical for respiratory syncytial virus-associated airway hyperreponsiveness through regulation by complement c3a and tachykinins. | respiratory syncytial virus (rsv) infection is associated with serious lung disease in infants and immunocompromised individuals and is linked to development of asthma. in mice, acute rsv infection causes airway hyperresponsiveness (ahr), inflammation, and mucus hypersecretion. infected cells induce complement activation, producing the anaphylatoxin c3a. in this paper, we show rsv-infected wild-type mice produce th17 cytokines, a response not previously associated with viral infections. mice def ... | 2011 | 21918196 |
| mortality burden of the a/h1n1 pandemic in mexico: a comparison of deaths and years of life lost to seasonal influenza. | the mortality burden of the 2009 a/h1n1 influenza pandemic remains controversial, in part because of delays in reporting of vital statistics that are traditionally used to measure influenza-related excess mortality. here, we compare excess mortality rates and years of life lost (yll) for pandemic and seasonal influenza in mexico and evaluate laboratory-confirmed death reports. | 2011 | 21976464 |
| clinical and radiological features of respiratory syncytial virus in solid organ transplant recipients: a single-center experience. | background: respiratory syncytial virus (rsv) infections range from upper respiratory illness to severe lower respiratory disease. there is no universally accepted treatment for rsv in solid organ transplant (sot) recipients. methods: retrospective review of adult sot patients with rsv infections, between january 2007 and december 2009, in a single transplant center was performed. results: during the 3-year period, a total of 24 adults developed rsv infection, including 12 (50%) sot recipients ( ... | 2011 | 22093238 |
| respiratory syncytial virus in neutrophils. | 2011 | 22207650 | |
| What happens when you mix a transplant with respiratory syncytial virus? | 2011 | 22211065 | |
| chest physiotherapy using passive expiratory techniques does not reduce bronchiolitis severity: a randomised controlled trial. | chest physiotherapy (cp) using passive expiratory manoeuvres is widely used in western europe for the treatment of bronchiolitis, despite lacking evidence for its efficacy. we undertook an open randomised trial to evaluate the effectiveness of cp in infants hospitalised for bronchiolitis by comparing the time to clinical stability, the daily improvement of a severity score and the occurrence of complications between patients with and without cp. children <1 year admitted for bronchiolitis in a t ... | 2011 | 21927808 |
| Respiratory viral infections in hematopoietic stem cell and solid organ transplant recipients. | Respiratory viral infections (RVIs) are common causes of mild illness in immunocompetent children and adults with rare occurrences of significant morbidity or mortality. Complications are more common in the very young, very old, and those with underlying lung diseases. However, RVIs are increasingly recognized as a cause of morbidity and mortality in recipients of hematopoietic stem cell transplants (HSCT) and solid organ transplants (SOTs). Diagnostic techniques for respiratory syncytial virus ... | 2011 | 21858751 |
| disease burden of the most commonly detected respiratory viruses in hospitalized patients calculated using the disability adjusted life year (daly) model. | the most common acute infections occur in the respiratory tract. recent discoveries of several novel viruses have markedly increased the repertoire of agents understood to cause presentations of acute respiratory disease. | 2011 | 21880543 |
| specific viruses detected in nigerian children in association with acute respiratory disease. | occurrence of different viruses in acute respiratory tract infections of nigerian children was examined. respiratory swabs were collected from 246 children referred to hospital clinics because of acute respiratory symptoms from february through may 2009. validated real-time rt-pcr techniques revealed nucleic acids of at least one virus group in 189 specimens (77%). human rhinoviruses and parainfluenza viruses were present each in one third of the children. adenoviruses, enteroviruses, human meta ... | 2011 | 22007241 |
| clinical and laboratory features of pertussis in infants at the onset of a california epidemic. | we report clinical characteristics and outcome of infants <3 months of age hospitalized with pertussis compared with viral respiratory infection (respiratory syncytial virus and influenza). patients with pertussis more often were afebrile, had more visits before admission, and had longer hospital stays. household coughing contacts were common. | 2011 | 21925678 |
| Nanobodies®: new ammunition to battle viruses. | In 1989, a new type of antibody was identified, first in the sera of dromedaries and later also in all other species of the Camelidae family. These antibodies do not contain a light chain and also lack the first constant heavy domain. Today it is still unclear what the evolutionary advantage of such heavy chain-only antibodies could be. In sharp contrast, the broad applicability of the isolated variable antigen-binding domains (VHH) was rapidly recognized, especially for the development of thera ... | 2011 | 21939690 |
| a frequentist approach to estimating the force of infection for a respiratory disease using repeated measurement data from a birth cohort. | this article aims to develop a probability-based model involving the use of direct likelihood formulation and generalised linear modelling (glm) approaches useful in estimating important disease parameters from longitudinal or repeated measurement data. the current application is based on infection with respiratory syncytial virus. the force of infection and the recovery rate or per capita loss of infection are the parameters of interest. however, because of the limitation arising from the study ... | 2011 | 22028340 |
| Respiratory viruses in children with cystic fibrosis: viral detection and clinical findings. | Please cite this paper as: Burns et al. (2011) Respiratory viruses in children with cystic fibrosis: viral detection and clinical findings. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2011.00292.x. Background Viral detection from different respiratory sample types in children with cystic fibrosis (CF) is facilitated by available molecular methods, but optimum sampling strategies have not been identified. In addition, associations between viral detection and respiratory symp ... | 2011 | 21955319 |
| protein kinase r is a novel mediator of cd40 signaling and plays a critical role in modulating immunoglobulin expression during respiratory syncytial virus infection. | effective immunoglobulin responses play a vital role in protection against most pathogens. however, the molecular mediators and mechanisms responsible for signaling and selective expression of immunoglobulin types remain to be elucidated. previous studies in our laboratory have demonstrated that protein kinase r (pkr) plays a crucial role in ige responses to double-stranded rna (dsrna) in vitro. in this study, we show that pkr plays a critical role in igg expression both in vivo and in vitro. pk ... | 2011 | 21994357 |
| respiratory syncytial virus hospitalization trends in infants with chronic lung disease of infancy, 1998-2008. | infants with chronic lung disease of infancy (cldi) are at high risk for severe respiratory syncytial virus (rsv) illness requiring hospitalization. palivizumab was first licensed in 1998 for the prevention of rsv disease in high-risk infants, including those with cldi. we performed a retrospective cohort study of all hospitalized children with cldi aged <2 years between 1998 and 2008 in the usa to determine trends in rates of hospitalizations due to rsv (rsvh) since the launch of palivizumab. | 2011 | 22003308 |
| Non-invasive sample collection for respiratory virus testing by multiplex PCR. | Identifying respiratory pathogens within populations is difficult because invasive sample collection, such as with nasopharyngeal aspirate (NPA), is generally required. PCR technology could allow for non-invasive sampling methods. | 2011 | 21855405 |
| comparison of four rt-pcr assays for detection of bovine respiratory syncytial virus. | rt-pcr assays for detection of brsv, based on four different sets of primers were optimized and evaluated for their sensitivity and specificity. primers used in this study were specific for genes encoding three brsv proteins, nucleoprotein n and glycoproteins f and g. our results indicated that rt-pcr with primers b7:b8 for g protein was the most efficient in detecting brsv. starters b7:b8 reacted specifically only with brsv strains, no cross-reaction with other closely related viruses to brsv w ... | 2011 | 21957740 |
| respiratory hospitalizations and respiratory syncytial virus prophylaxis in special populations. | palivizumab utilization, compliance, and outcomes were examined in infants with preexisting medical diseases within the canadian registry database (caress) to aid in developing guidelines for potential "at-risk" infants in the future. infants who received ≥1 dose of palivizumab during the 2006-2010 respiratory syncytial virus (rsv) seasons at 29 sites were recruited and utilization, compliance, and outcomes related to respiratory infection/illness (ri) events were collected monthly. hazard ratio ... | 2011 | 22203430 |
| Krüppel-like factor 6 regulates transforming growth factor-ß gene expression during human respiratory syncytial virus infection. | Human respiratory syncytial virus (RSV) infection is associated with airway remodeling and subsequent asthma development. Transforming growth factor-beta (TGF) plays a crucial role in asthma development. The mechanism regulating TGF gene expression during RSV infection is not known. Kruppel-like factor family of transcription factors are critical regulators of cellular/tissue homeostasis. Previous studies have shown that Kruppel-like factor 6 (KLF6) could function as a trans-activator of TGF gen ... | 2011 | 21849067 |
| renal transplant with bronchiolitis obliterans organizing pneumonia (boop) attributable to tacrolimus and herpes simplex virus (hsv) pneumonia. | background: solid organ transplants (sots) may be complicated by a wide variety of infectious and noninfectious pulmonary disorders. transplant patients receive immunosuppressive drugs to prevent rejection, but these drugs also predispose them to infection. because immunosuppressive therapy impairs t-lymphocyte function, ie, cell-mediated immunity, such therapy, not surprisingly, predisposes patients to intracellular pulmonary pathogens. community-acquired pneumonia (cap) in patients with sot us ... | 2011 | 21996615 |
| Occult serious bacterial infection in infants younger than 60 to 90 days with bronchiolitis: a systematic review. | To summarize the risk of occult serious bacterial infection in the youngest febrile infants presenting with either clinical bronchiolitis or respiratory syncytial virus infection. | 2011 | 21969396 |
| [In Process Citation]. | 2011 | 21971620 | |
| Prenatal prevention of respiratory syncytial virus bronchiolitis. | 2011 | 21905778 | |
| Respiratory syncytial virus and the premature infant parent. | 2011 | 21846631 | |
| rela ser276 phosphorylation-coupled lys310 acetylation controls transcriptional elongation of inflammatory cytokines in respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is a negative-sense single-stranded rna virus responsible for lower respiratory tract infections (lrtis) in humans. in experimental models of rsv lrti, the actions of the nuclear factor κb (nf-κb) transcription factor mediate inflammation and pathology. we have shown that rsv replication induces a mitogen-and-stress-related kinase 1 (msk-1) pathway that activates nf-κb rela transcriptional activity by a process involving serine phosphorylation at serine (ser) re ... | 2011 | 21900162 |
| phosphorylation of the human respiratory syncytial virus n protein provokes a decrease in viral rna synthesis. | when hep-2 cells are infected by human respiratory syncytial virus (hrsv) its n protein becomes phosphorylated at tyrosine (y) y38, in a strictly regulated way. to determine how this phosphorylation affects nucleocapsid (nc) template activity during viral rna synthesis, n protein variants were analysed in which y38 and nearby y residues were substituted by phenylalanine (f; y23f, y38f and y69f) or aspartic acid (d; y23d and y38d). while the capacity of these proteins to form the nc and to intera ... | 2011 | 22019509 |
| equal virulence of rhinovirus and respiratory syncytial virus in infants hospitalized for lower respiratory tract infection. | respiratory syncytial virus (rsv) and rhinovirus (rv) are predominant viruses associated with lower respiratory tract infection in infants. we compared the symptoms of lower respiratory tract infection caused by rsv and rv in hospitalized infants. rv showed the same symptoms as rsv, so on clinical grounds, no difference can be made between these pathogens. no relation between polymerase chain reaction cycle threshold value and length of hospital stay was found. | 2011 | 21909047 |
| the prevalance of respiratory viruses among healthcare workers serving pilgrims in makkah during the 2009 influenza a (h1n1) pandemic. | despite the high risk of acquiring respiratory infections, healthcare workers who treat pilgrims at hajj have not been studied in previous research on respiratory diseases during hajj. the objective of this study was to determine the prevalence of different respiratory viruses among healthcare workers who treated pilgrims during hajj 2009, the year of the influenza a h1n1 pandemic. a cross-sectional study was performed just before and after hajj (25-29 november, 2009). nasal and throat swabs wer ... | 2011 | 22197024 |
| Communicable diseases prioritized for surveillance and epidemiological research: results of a standardized prioritization procedure in Germany, 2011. | To establish strategic priorities for the German national public health institute (RKI) and guide the institute's mid-term strategic decisions, we prioritized infectious pathogens in accordance with their importance for national surveillance and epidemiological research. | 2011 | 21991334 |
| Virological and clinical characterization of respiratory infections in children attending an emergency department during the first autumn-winter circulation of pandemic A (H1N1) 2009 influenza virus. | Clin Microbiol Infect ABSTRACT: To characterize respiratory virus infections during the first autumn-winter season of pandemic A (H1N1) 2009 influenza virus (A/H1N1/2009) circulation, a prospective study in children attending a paediatric emergency department at the Sapienza University hospital, Rome, was conducted from November 2009 to March 2010. By means of both nasal washings and pharyngeal swabs, enrolled children were checked for 14 respiratory viruses. The majority of acute respiratory in ... | 2011 | 21923780 |
| future opportunities for passive immunity against viral diseases. | 2011 | 21998473 | |
| infections caused by rsv among children and adults during two epidemic seasons. | respiratory syncytial virus (rsv) is one of the most common causes of lower respiratory tract infections in young children, immunocompromised patients (children and adults), patients with chronic respiratory diseases and elderly people. reinfections occur throughout the life, but the severity of disease decreased with subsequent infection. the aim of this study was to analyze the frequency of rsv infections in two selected subpopulations: young children (below 5 y.) and adults with chronic respi ... | 2011 | 22184933 |
| flavone c-glycosides from the leaves of lophatherum gracile and their in vitro antiviral activity. | four new flavone c-glycosides, luteolin 6- c- α-l-arabinopyranosyl-7- o- β-d-glucopyranoside ( 1), apigenin 6- c- β-d-galactopyranosiduronic acid (1 → 2)- α-l-arabinopyranoside ( 2), luteolin 6- c- β-d-galactopyranosiduronic acid (1 → 2)- α-l-arabinopyranoside ( 3), and luteolin 6- c- β-d-glucopyranosiduronic acid (1 → 2)- α-l-arabinofuranoside ( 4), along with three known ones, were isolated from the leaves of lophatherum gracile. the structures of the new compounds were elucidated by extensi ... | 2011 | 21870321 |
| clinical and epidemiological characteristics of respiratory syncytial virus and influenza virus associated hospitalization in urban thai infants. | respiratory syncytial virus (rsv) and influenza infections are among the leading cause of hospitalized lower respiratory tract infections (lrti) in children especially among those younger than 1 year of age. few descriptions of these 2 important viruses in thai children less than 1 year of age have been published. | 2011 | 22043771 |
| emerging antibody products and nicotiana manufacturing. | antibody based products are not widely available to address multiple global health challenges due to high costs, limited manufacturing capacity, and long manufacturing lead times. nicotiana-based manufacturing of antibody products may now begin to address these challenges as a result of revolutionary advances in transient expression and altered glycosylation pathways. this review provides examples of emerging antibody-based products (mucosal and systemic) that could be competitive and commercial ... | 2011 | 21358287 |
| liquid chromatographic resolution of 3-amino-1,4-benzodiazepin-2-one derivatives on various pirkle-type chiral stationary phases. | the two enantiomers of n-acyl amide and n-ureide derivatives of 3-amino-5-phenyl-1,4-benzodiazepin-2-ones, which have been known to show anti-respiratory syncytial virus (rsv) activity, were resolved on seven different pirkle-type chiral stationary phases (csps) with the use of 10% isopropyl alcohol in hexane as a mobile phase. among the seven pirkle-type csps, the one based on (s)-leucine derivative named as n-phe-l-leu was found to be most successful, the separation factors (a) and the resolut ... | 2011 | 21365690 |
| establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species. | a sheep kidney-derived cell line, flk-n3, was successfully established after serial (>100) passages. persistent infection of this cell line with viruses and mycoplasma was not detected. the cells grew well and showed susceptibility to a wide variety of viruses derived from ovine, bovine, and porcine species, including orf virus, maedi visna virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine viral diarrhea viruses 1 and 2, bovine coronavirus, bovine respiratory syncytial virus, bov ... | 2011 | 21695582 |
| characterization of human metapneumovirus from pediatric patients with acute respiratory infections in a 4-year period in beijing, china. | human metapneumovirus (hmpv) was discovered by scientists in the netherlands as a novel respiratory virus in 2001 and had been found in children with acute respiratory tract infections (arti) in china. the objective of this study was to determine the importance of hmpv infection in children in beijing and the genotypes of the circulating virus by the surveillance during a four-consecutive-year period. | 2011 | 21740766 |
| respiratory syncytial virus binds and undergoes transcription in neutrophils from the blood and airways of infants with severe bronchiolitis. | neutrophils are the predominant cell in the lung inflammatory infiltrate of infants with respiratory syncytial virus (rsv) bronchiolitis. although it has previously been shown that neutrophils from both blood and bronchoalveolar lavage (bal) are activated, little is understood about their role in response to rsv infection. this study investigated whether rsv proteins and mrna are present in neutrophils from blood and bal of infected infants. | 2011 | 21742845 |
| Preschool asthma after bronchiolitis in infancy. | Asthma risk is lower after wheezing associated with RSV than with non-RSV infection in infancy. RSV is the main wheezing-associated virus in infants aged <6 months. We evaluated the outcome of children hospitalised for bronchiolitis at <6 months of age, with special focus on viral etiology and early risk factors. Out of 205 infants hospitalised for bronchiolitis at <6 months of age, 127 (62%) attended the control visit at age (mean) 6.5 years, and the parents of additional 39 children were inter ... | 2011 | 21700604 |
| respiratory syncytial virus prevention in children with congenital heart disease: who and how? | respiratory syncytial virus (rsv) is a major cause of respiratory infection in children. most of the pediatric population have rsv infection before the age of 2, and recurrent infections are common even within one season. chronic lung disease, prematurity, along with congenital heart disease (chd) are major risk factors in severe lower respiratory infection. in hemo-dynamically significant chd patients with rsv infection, hospitalization is usually needed and the possibility of treatment in inte ... | 2011 | 21829410 |
| RSV 2010: Recent advances in research on respiratory syncytial virus and other pneumoviruses. | Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) are important causes of acute respiratory tract disease in infants, immunocompromised patients and the elderly. The Seventh International RSV symposium was held in Rotterdam, the Netherlands, from December 2-5, 2010. This symposium is the flagship event for leading investigators engaged in RSV and HMPV research around the world. The objective of the symposium was to provide a forum to review recent advances in research on RSV, HM ... | 2011 | 21827813 |
| comparative dynamics, morbidity and mortality burden of pediatric viral respiratory infections in an equatorial city. | : although acute respiratory infections (aris) are the global leading cause of pediatric morbidity and mortality, the relative impact of viral pathogens on pediatric aris is still poorly understood, especially in equatorial settings. long-term studies of multiple viruses concurrently circulating in these regions are still lacking. here, we report the results of a systematic prospective surveillance of multiple respiratory viruses conducted every weekday for nearly a decade in an equatorial city ... | 2011 | 22001966 |
| rna viruses in young nepalese children hospitalized with severe pneumonia. | pneumonia is among the leading causes of illness and death in children <5 years of age worldwide. there is little information on the viral etiology of severe pneumonia in low-income countries, where the disease burden is particularly high. | 2011 | 21860338 |
| bovine respiratory syncytial virus iscoms-immunity, protection and safety in young conventional calves. | bovine respiratory syncytial virus (brsv) is a major cause of bronchiolitis and pneumonia in cattle and causes yearly outbreaks with high morbidity in europe. commercial vaccines against this virus needs improvement of efficacy, especially in calves with brsv-specific maternally derived antibodies (mda). we previously reported that an experimental brsv-iscom vaccine, but not a commercial vaccine, induced strong clinical and virological protection in calves with mda, immunized at 7-15 weeks of ag ... | 2011 | 21864616 |
| a study of the genetic variability of human respiratory syncytial virus (hrsv) in cambodia reveals the existence of a new hrsv group b genotype. | human respiratory syncytial virus (hrsv) is the leading cause of hospitalization of children aged <5 years due to respiratory illness in industrialized countries, and pneumonia is the leading cause of mortality among children aged <5 years worldwide. although hrsv was first identified in 1956, a preventative vaccine has yet to be developed. here we report the results of the first study to investigate the circulation and genetic diversity of hrsv in cambodia among an all-ages population over 5 co ... | 2011 | 21865418 |
| the effects of hourly differences in air pollution on the risk of myocardial infarction: case crossover analysis of the minap database. | to investigate associations between air pollution levels and myocardial infarction (mi) on short timescales, with data at an hourly temporal resolution. | 2011 | 21933824 |