Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| inhibitory effect of ferulic acid and isoferulic acid on the production of macrophage inflammatory protein-2 in response to respiratory syncytial virus infection in raw264.7 cells. | we investigated the effect of ferulic acid (fa) and isoferulic acid (ifa), which are the main active components of the rhizoma of cimicifuga heracleifolia (ch), an anti-inflammatory drug used frequently in japanese traditional medicine, on the production of macrophage inflammatory protein-2 (mir-2) in a murine macrophage cell line, raw264.7, in response to respiratory syncytial virus (rsv) infection. following the exposure of cells to rsv for 20h, the mip-2 level in condition medium was increase ... | 1999 | 10704056 |
| tumor necrosis factor-alpha and interleukin-6 profiles in children with pneumonia. | pneumonia is a common cause of hospitalization and is associated with high morbidity in children. tumor necrosis factor-alpha (tnf-alpha) and interleukin-6 (il-6) are primary mediators of inflammation, and have been implicated in a large number of infectious and non-infectious inflammatory diseases. the serum concentrations of tnf-alpha and il-6 were measured by enzyme-linked immunosorbent assay (elisa) in 27 patients with bacterial pneumonia (n = 12) or respiratory syncytial virus (rsv) pneumon ... | 1999 | 10650487 |
| prenatal orogastric gene delivery results in transduction of the small bowel in the fetal rabbit. | gene therapy for meconium ileus, or other genetic diseases involving the gastrointestinal epithelium, may be possible with prenatal delivery of the cftr gene to the gastrointestinal tract. although minimally invasive techniques will probably be used for any future therapy of gastrointestinal disease, it is important to first test this strategy with a reliable animal model. | 1999 | 10640868 |
| update: respiratory syncytial virus activity--united states, 1998-1999 season. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract disease in infants and young children worldwide. in temperate climates, rsv infections occur primarily during annual outbreaks, which peak during winter months . in the united states, rsv activity is monitored by the national respiratory and enteric virus surveillance system (nrevss), a voluntary, laboratory-based system. this report summarizes trends in rsv activity reported to nrevss during july 1998 june 199 ... | 1999 | 10632299 |
| the outcome of 26 patients with respiratory syncytial virus infection following allogeneic stem cell transplantation. | respiratory syncytial virus (rsv) is known to cause acute lung injury in the immunocompromised host, especially recipients of bone marrow allografts. specific prognostic factors for the development of severe life-threatening disease remain to be identified as does the optimum treatment of established disease. over a 5-year period the incidence and outcome of rsv in bmt recipients was analysed retrospectively. prognostic factors assessed included type of transplant, engraftment status at the time ... | 1999 | 10627641 |
| mortality following isolation of various respiratory viruses in nursing home residents. | to compare mortality following isolation of influenza a to mortality following isolation of other respiratory viruses in a nursing home. | 1999 | 10614604 |
| rsv infection in infants and young children. what's new in diagnosis, treatment, and prevention? | rsv is the most important respiratory pathogen in infants and young children. about 1% of primary rsv infections result in hospitalization. the virus is spread by large droplets of secretions or contact with contaminated secretions. infants infected with rsv may demonstrate poor feeding, rhinorrhea, apnea, lethargy, wheezing, and respiratory distress. diagnosis may be made by clinical signs and symptoms (especially those observed during epidemics), by chest radiographs showing hyperinflation, or ... | 1999 | 10608968 |
| [recommendations for immunologic prevention of respiratory syncytial virus (rsv) infections]. | 1999 | 10612193 | |
| cetirizine counter-regulates interleukin-8 release from human epithelial cells (a549) | cetirizine, a h1-receptor antagonist, exerts besides its well-known anti-allergic potential an array of anti-inflammatory activities. in particular epithelial cells activated in the presence of cetirizine showed a reduced icam-1 cell surface expression and a diminished release of sicam-1. | 1999 | 10594545 |
| effects of respiratory syncytial virus persistence on airway responsiveness and inflammation in guinea-pigs. | recurrent wheezing and asthma often develop after acute respiratory syncytial virus (rsv) bronchiolitis, but the mechanisms of these sequelae are poorly understood. using a guinea-pig model of human rsv lung infection, the effects of long-term viral persistence on three hallmarks of asthma: nonspecific airway responsiveness, airway inflammation and airway remodelling were examined. guinea-pigs were studied 100 days after intranasal instillation of either human rsv or uninfected vehicle, using: 1 ... | 1999 | 10596691 |
| respiratory syncytial virus: an underestimated cause of respiratory infection, with prospects for a vaccine. | respiratory syncytial virus (rsv) infects most people by the time they are 2 years old and reinfects throughout life. rsv is best recognised for causing bronchiolitis in infants--it is one of the most important respiratory pathogens in childhood in industrialised countries. the clinical manifestations of rsv infection in adults and elderly people, from upper respiratory tract infection to pneumonia, are less well known. part of the burden of winter mortality in elderly people is attributable to ... | 1999 | 10598379 |
| efficient gene transfer and long-term expression in neurons using a recombinant adenovirus with a neuron-specific promoter. | adenoviruses are highly efficient vectors for gene transfer into brain cells. restricting transgene expression to specific cell types and maintaining long-term expression are major goals for gene therapy in the central nervous system. we targeted gene expression to neurons by constructing an adenoviral vector that expressed the e. coli lacz reporter gene under the control of the rat neuron-specific enolase promoter (ad-nse). expression from ad-nse was compared with that from an adenoviral vector ... | 1999 | 10602384 |
| [prophylaxis of respiratory syncytial virus (rsv) in preterm infants with/without bronchopulmonary dysplasia: hyperimmune globulin (rsv-igiv) and palivizumab (medi-493)]. | respiratory syncytial virus (rsv) causes seasonal epidemics between december and march (april) and remains the main agent that causes severe lower respiratory tract infections in young infants. children with bronchopulmonary dysplasia up to 24 months of age and preterm infants with a gestational age of 32 weeks and below, who are less than six months of age, are at highest risk for severe rsv infection. rsv-igiv has been demonstrated to reduce significantly rsv associated hospitalizations, rsv a ... | 1999 | 10592925 |
| molecular epidemiology of respiratory syncytial virus in the gambia. | respiratory syncytial virus (rsv) infection in the gambia occurs seasonally in association with the rainy season. this study examined the genetic variability of rsv isolates from four consecutive epidemics from 1993-6. each epidemic was made up of a number of variants which were replaced in subsequent epidemics. analysis of attachment (g) protein gene sequences showed that isolates were closely related to those observed in the rest of the world. however, many isolates from 1993 and 1994 were unl ... | 1999 | 10098799 |
| efficacy of rd3-0028 aerosol treatment against respiratory syncytial virus infection in immunosuppressed mice. | rd3-0028, a benzodithiin compound, has antiviral activity against respiratory syncytial virus (rsv) in cell culture. we used a mouse model of rsv infection to determine the in vivo effect of rd3-0028. cyclophosphamide (cyp)-treated, immunosuppressed mice were inoculated intranasally. the lungs of the mice were removed on day 4. the virus titers of the lungs of rd3-0028-treated mice were compared to the virus titers of the lungs of virus-inoculated, untreated control mice. in an effort to increas ... | 1999 | 10103176 |
| bronchiectasis and bronchiolitis obliterans post respiratory syncytial virus infection: think again. | respiratory syncytial virus (rsv) lower respiratory tract infections are common, yet long-term sequelae in previously healthy infants and children are rarely reported. we report a child who developed bronchiectasis and bronchiolitis obliterans following an rsv lower respiratory tract infection but in whom adenovirus was detected on stored serum. co-infection with adenovirus is the likely cause for the bronchiectasis and bronchiolitis obliterans. | 1999 | 10571767 |
| [respiratory syncytial virus pneumonia in four immunocompromised adults]. | in hematologic malignancies, respiratory syncytial viral infections can be explained by neutropenia, and cellular and humoral immunodepression, and may cause severe respiratory infections. | 1999 | 10573730 |
| activation of presynaptic camp-dependent protein kinase is required for induction of cerebellar long-term potentiation. | cerebellar long-term potentiation (ltp) is a persistent increase in the strength of the granule cell-purkinje neuron synapse that occurs after brief stimulation of granule cell axons at 2-8 hz. previous work has indicated that cerebellar ltp induction requires presynaptic ca influx, stimulation of ca-sensitive adenylyl cyclase, and activation of pka. the evidence implicating pka has come from bath application of drugs during ltp induction, an approach that does not distinguish between pka activa ... | 1999 | 10575019 |
| active and passive immunisation against respiratory syncytial virus. | rsv is a major cause of respiratory illness in infants under 2 years of age. evidence is accumulating that it is also underestimated as a cause of respiratory infection in adults, the elderly and immunocompromised individuals. active interventions to control the impact of rsv infection have been hampered by a lack of understanding of the immune response to rsv in different age groups. a number of different strategies for developing rsv vaccines have been pursued, including live attenuated vaccin ... | 1999 | 10578118 |
| complications of pediatric laryngotracheal reconstruction: prevention strategies. | a retrospective chart review was performed to quantify the postoperative complications and outcomes of 82 consecutive cases of laryngotracheal reconstruction (ltr) and cricotracheal resection (ctr) performed at a pediatric tertiary care hospital over the last 9 years. six cases of respiratory syncytial virus (rsv) bronchiolitis and 8 cases of cervical pseudomonal wound abscess (pwa) were identified in a total of 12 patients. all of these infections occurred after single-stage ltr or ctr. both rs ... | 1999 | 10579227 |
| epidemiology of respiratory syncytial virus infection among paediatric patients in hong kong: seasonality and disease impact. | in a 5-year retrospective survey of respiratory syncytial virus (rsv) infections among hospitalized children, 1340 cases were identified of which, 98.4% were children < 5 years old with a male:female ratio of 1.5: 1. most cases occurred from april to september showing a significant positive correlation with temperature and relative humidity. community-acquired infections accounted for 92.5% of the cases with a mean hospital stay of 5 days. the estimated annual incidence of rsv infection requirin ... | 1999 | 10579445 |
| stable transfection of rat preporinsulin ii gene into rat hematopoietic stem cells via recombinant adeno-associated virus. | we investigated the ability of recombinant adeno-associated virus (raav), to mediate the transfer of rat preproinsulin ii (ri2) gene into rat hematopoietic stem cells in vitro and expression of ri2 following intra-venous (i.v.) injection of infected stem cells into syngeneic rats. the plp-1 recombinant plasmid containing ri2 was engineered as follows: ri2 with rsv-promoter was released from pbc 12bi (atcc), purified, and inserted into bamh1 site of raav vector plasmid pwp-19. plasmid plp-1, toge ... | 1999 | 10579458 |
| the epidemiology of respiratory syncytial virus (rsv) infections in south african children. | to review the incidence, outcomes and risk factors associated with respiratory syncytial virus (rsv) infection in south african children. | 1999 | 10582064 |
| prednisolone treatment of respiratory syncytial virus infection: a randomized controlled trial of 147 infants. | to evaluate the effect of systemic prednisolone as an adjunct to conventional treatment with beta2-agonist, respiratory support, and fluid replacement in hospitalized infants <24 months of age with respiratory syncytial virus (rsv) infection. | 1999 | 10586011 |
| respiratory illness after severe respiratory syncytial virus disease in infancy in the gambia. | to determine the frequency of later respiratory tract morbidity after respiratory syncytial virus (rsv) disease in infancy. | 1999 | 10586169 |
| comparison of vidas with direct immunofluorescence for the detection of respiratory syncytial virus in clinical specimens. | requirements for infection control measures and the decision for treatment with antiviral agents make the rapid detection of respiratory syncytial virus (rsv) essential for hospitalized, pediatric and immunocompromised patients. immunofluorescence is considered to be the most rapid and sensitive method for direct detection of rsv in clinical specimens, but several enzyme-linked immunoassays have also been commercially available. | 1999 | 10588455 |
| plasma surfactant protein-b is elevated in infants with respiratory syncytial virus-induced bronchiolitis. | respiratory syncytial virus (rsv) is the most frequent cause of bronchiolitis. however the pathophysiology of bronchiolitis is unclear. leukocytes, especially neutrophils, may play an important role in the pathogenesis of bronchiolitis. whereas we have previously shown that neutrophils augment epithelial leakage and detachment in rsv infection in vitro, it is unknown whether epithelial damage occurs in vivo in infants with rsv bronchiolitis. we hypothesized that respiratory epithelial damage occ ... | 1999 | 10590031 |
| a simple and reproducible method for collecting nasal secretions in frail elderly adults, for measurement of virus-specific iga. | the standard method for collection of respiratory secretions, by use of a nasal wash (nw) to measure virus-specific iga, is problematic in frail elderly adults. therefore, a simplified collection approach using a nasal swab (ns) is described. nw and ns samples were collected from healthy young and frail elderly adults, and iga titers to respiratory syncytial virus (rsv) fusion and attachment glycoproteins were determined by enzyme immunoassay. correlation between iga titers in nw and ns was exce ... | 1999 | 10191235 |
| surfactant protein levels in severe respiratory syncytial virus infection. | infection with respiratory syncytial virus (rsv) is a common cause of respiratory disease in infancy. surfactant phospholipids have been shown to be reduced in severe rsv infection. reduction in surfactant proteins might also contribute to the pathogenesis of this disease. we investigated daily levels of surfactant proteins in bronchoalveolar lavage (bal) fluid from 18 ventilated infants with rsv infection (median age 3.1 mo) and in a control group of 16 ventilated surgical patients (median age ... | 1999 | 10194154 |
| severity of illness models for respiratory syncytial virus-associated hospitalization. | the objective of this investigation was to examine the feasibility of multivariate severity of illness models for pediatric patients hospitalized with respiratory syncytial virus (rsv) infection. from a preexisting retrospective cohort study database, all infants and children 2 yr of age or younger with community-acquired rsv infection admitted to the university of michigan's c. s. mott children's hospital during nine epidemics were examined. the study group consisted of 802 hospitalized patient ... | 1999 | 10194171 |
| respiratory syncytial virus infection: immune response, immunopathogenesis, and treatment. | respiratory syncytial virus (rsv) is the single most important cause of lower respiratory tract infection during infancy and early childhood. once rsv infection is established, the host immune response includes the production of virus-neutralizing antibodies and t-cell-specific immunity. the humoral immune response normally results in the development of anti-rsv neutralizing-antibody titers, but these are often suboptimal during an infant's initial infection. even when the production of rsv neut ... | 1999 | 10194461 |
| surfactant protein-a enhances respiratory syncytial virus clearance in vivo. | to determine the role of surfactant protein-a(sp-a) in antiviral host defense, mice lacking sp-a (sp-a-/-) were produced by targeted gene inactivation. sp-a-/- and control mice (sp-a+/+) were infected with respiratory syncytial virus (rsv) by intratracheal instillation. pulmonary infiltration after infection was more severe in sp-a-/- than in sp-a+/+ mice and was associated with increased rsv plaque-forming units in lung homogenates. pulmonary infiltration with polymorphonuclear leukocytes was g ... | 1999 | 10194474 |
| risk factors for severe respiratory syncytial virus infection leading to hospital admission in children in the western region of the gambia. | acute lower respiratory tract infections (alri) are the major cause of mortality and morbidity in young children worldwide. respiratory syncytial virus (rsv) infection is the most important viral cause of severe alri but only a small proportion of children infected with this virus develop severe disease. to identify possible risk factors for severe rsv infection leading to hospital admission we have carried out a case-control study of gambian children with rsv infection admitted to hospital. | 1999 | 10195682 |
| interferon gamma production during bovine respiratory syncytial virus (brsv) infection is diminished in calves vaccinated with formalin-inactivated brsv. | formalin-inactivated respiratory syncytial virus (fi-rsv) vaccination has been associated with severe disease in humans. research in mice suggests that fi-rsv may prime for decreased interferon gamma (ifn-gamma) production at subsequent infection. interferon-gamma production by peripheral blood mononuclear cells (pbmc) was measured following challenge of calves vaccinated with fi-brsv to determine whether a similar mechanism is operative in a host naturally susceptible to rsv. eight-week old mal ... | 1999 | 10195762 |
| the two amino acid substitutions in the l protein of cpts530/1009, a live-attenuated respiratory syncytial virus candidate vaccine, are independent temperature-sensitive and attenuation mutations. | cpts530/1009 is a live-attenuated, temperature-sensitive (ts) rsv vaccine candidate that was shown previously to be attenuated for seronegative humans. it was generated by two rounds of chemical mutagenesis: first, a partially attenuated, cold-passaged (cp), non-ts rsv mutant (cprsv) was mutagenized to yield the ts derivative cpts530, and then cpts530 was mutagenized to yield cpts530/1009, which is more ts. previous nucleotide (nt) sequence analysis of cpts530 showed that it has a single nt chan ... | 1999 | 10195777 |
| experimental gene therapy against subcutaneously implanted glioma with a herpes simplex virus-defective vector expressing interferon-gamma. | we investigated the feasibility of local treatment or tumor vaccination with a herpes simplex virus (hsv) type 1-defective vector. the vector was engineered to express murine interferon-gamma (ifn-gamma) for experimental gene therapy against mouse glioma rous sarcoma virus (rsv). the murine ifn-gamma gene was driven by the cytomegalovirus promoter. the helper virus (tsk) was thermosensitive; consequently, this vector could only proliferate at 31 degrees c. a high level of murine ifn-gamma expres ... | 1999 | 10195881 |
| incomplete regulation of nf-kappab by ikappabalpha during respiratory syncytial virus infection in a549 cells. | respiratory syncytial virus (rsv) infection of airway epithelial cells results in persistent nf-kappab activation and nf-kappab-mediated interleukin-8 production. previous studies in airway epithelial cells demonstrated that tumor necrosis factor alpha (tnf-alpha)-induced nf-kappab activation is transient due to regulation by ikappabalpha. however, during rsv infection, ikappabalpha has only a partial inhibitory effect on nf-kappab activation. studies presented here demonstrate that neither incr ... | 1999 | 10196356 |
| reducing the rate of nosocomially transmitted respiratory syncytial virus. | a large number (17) of nosocomial respiratory syncytial virus cases led to the development of control measures to prevent transmission of respiratory syncytial virus (rsv) within the johns hopkins hospital's children's center. | 1999 | 10196485 |
| sequential mri, spect and pet in respiratory syncytial virus encephalitis. | we report on a 3-year-old girl with respiratory syncytial virus (rsv) encephalitis manifested by disturbance of consciousness, conjugate eye deviation, anuria, truncal ataxia and intention tremor. t2-weighted magnetic resonance imaging (mri) showed hyperintense areas in the cerebellar cortex. no lesion was detected in the cerebral cortex, pons or spinal cord. the hyperintense areas in the cerebellar cortex diminished with recovery from the clinical manifestations and had resolved 2 months after ... | 1999 | 10199908 |
| cd8 t cells are essential in the development of respiratory syncytial virus-induced lung eosinophilia and airway hyperresponsiveness. | viral respiratory infections can cause bronchial hyperresponsiveness and exacerbate asthma. in mice, respiratory syncytial virus (rsv) infection results in airway hyperresponsiveness (ahr) and eosinophil influx into the airways. the immune cell requirements for these responses to rsv infection are not well defined. to delineate the role of cd8 t cells in the development of rsv-induced ahr and lung eosinophilia, we tested the ability of mice depleted of cd8 t cells to develop these symptoms of rs ... | 1999 | 10201948 |
| cloning and expression of bovine herpesvirus-1 glycoprotein c. | glycoprotein c (gc) of bovine herpesvirus-1 (bhv-1) is expressed at high levels on surface of infected cells and on virus envelope. it is relatively immunodominant in antibody response to bhv-1 infection and protective in immunized bovines against bhv-1 challenge. in an attempt to express gc in mammalian cells, the 2.4 kb bamhi-ecori fragment, containing complete coding sequence of the gc gene was excised from a recombinant plasmid and cloned under the control of rsv-ltr. the resultant plasmid p ... | 1999 | 10205673 |
| cooperation between phosphorylation and acetylation processes in transcriptional control. | we previously reported that the activation of the m promoter of the human choline acetyltransferase (chat) gene by butyrate and trapoxin in transfected chp126 cells is blocked by pd98059, a specific mitogen-activated protein kinase kinase (mek) inhibitor (e. espinos and m. j. weber, mol. brain res. 56:118-124, 1998). we now report that the transcriptional effects of histone deacetylase inhibitors are mediated by an h7-sensitive serine/threonine protein kinase. activation of the chat promoter by ... | 1999 | 10207071 |
| induction of cd95 (fas) and apoptosis in respiratory epithelial cell cultures following respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection is associated with epithelial cell death and vigorous inflammation. in mouse models, and in immunosuppressed patients, cd8(+) t cells are necessary for rsv clearance. in vitro, rsv has been shown to induce expression of several proteins on the respiratory epithelial cell, including rsv proteins, icam-1, and mhc class i, that can potentially interact with cd8(+) t cells in initiating apoptosis of the target cell. one mechanism of t-cell-directed cell de ... | 1999 | 10208933 |
| tracheal aspirate as a substrate for polymerase chain reaction detection of viral genome in childhood pneumonia and myocarditis. | infectious respiratory disorders are important causes of childhood morbidity and mortality. viral causes are common and may lead to rapid deterioration, requiring mechanical ventilation; myocardial dysfunction may accompany respiratory decompensation. the etiologic viral diagnosis may be difficult with classic methods. the purpose of this study was to evaluate polymerase chain reaction (pcr) as a diagnostic method for identification of causative agents. | 1999 | 10209006 |
| comparison of long-term survival of cytomegalovirus promotre versus rous sarcoma virus promoter-driven thymidine kinase gene therapy in nude mice bearing human ovarian cancer. | the cytomegalovirus (cmv) promoter is considered one of the strongest positive regulators leading to expression of higher levels of the thymidine kinase (tk) enzyme than the rous sarcoma virus (rsv) promoter in vitro and in vivo. cell killing efficacy of adenovirus-mediated cmv promoter-driven herpes simplex virus (hsv) tk gene therapy has been found to be 2 to 10 times more effective than rsv driven hsv-tk gene therapy in vitro. in this study the impact of cmv- versus rsv-driven hsv-tk gene the ... | 1999 | 10211795 |
| [the efficacy of oral vitamin a supplementation for measles and respiratory syncytial virus (rsv) infection]. | recently, the efficacy of oral vitamin a supplementation for measles and respiratory syncytial (rsv) infection has been evaluated in developing countries. however, in developed countries where vitamin a deficiency is little worth consideration, few studies have been conducted on the effect of vitamin a supplementation. the effect of oral vitamin a (100,000 iu) supplementation was evaluated in 105 children with measles (age 5 months to 4 years) and in 96 children with rsv infection (ages a month ... | 1999 | 10213986 |
| safety and pharmacokinetics of an intramuscular monoclonal antibody (sb 209763) against respiratory syncytial virus (rsv) in infants and young children at risk for severe rsv disease. | we conducted a multicenter, double-blind, placebo-controlled, randomized trial of a humanized monoclonal antibody against a respiratory syncytial virus (rsv) fusion protein (sb 209763) to evaluate its safety, pharmacokinetics, and fusion inhibition and neutralization titers. forty-three infants who were either delivered prematurely (</=35 weeks' gestation) or exhibited bronchopulmonary dysplasia were administered either single or repeat (two doses, 8 weeks apart) intramuscular injections of sb 2 ... | 1999 | 10223933 |
| protective efficacy against respiratory syncytial virus following murine neonatal immunization with bbg2na vaccine: influence of adjuvants and maternal antibodies. | alum-adsorbed bbg2na, a recombinant vaccine derived in part from the respiratory syncytial virus (rsv) subgroup a g protein, induced moderate antibody titers after 1 immunization in 1-week-old mice but conferred complete lung protection upon rsv challenge. the anti-bbg2na igg1-igg2a neonatal isotype profile was suggestive of dominant th2 responses compared with those in adults. formulation of bbg2na with a th1-driving adjuvant efficiently shifted neonatal responses toward a more balanced and adu ... | 1999 | 10228051 |
| an in vitro selected binding protein (affibody) shows conformation-dependent recognition of the respiratory syncytial virus (rsv) g protein. | using phage-display technology, a novel binding protein (z-affibody) showing selective binding to the rsv (long strain) g protein was selected from a combinatorial library of a small alpha-helical protein domain (z), derived from staphylococcal protein a (spa). biopanning of the z-library against a recombinant fusion protein comprising amino acids 130-230 of the g protein from rsv-subgroup a, resulted in the selection of a z-affibody (z(rsv1)) which showed g protein specific binding. using biose ... | 1999 | 10231093 |
| a single amino acid substitution in the phosphoprotein of respiratory syncytial virus confers thermosensitivity in a reconstituted rna polymerase system. | the single amino acid change gly172 to ser in the phosphoprotein (p) of respiratory syncytial virus (rsv) has previously been shown to be responsible for the thermosensitivity and protein-negative phenotype of tsn19, a mutant of the b subgroup rsn-2 strain. this single change was inserted into the p gene of the a subgroup virus rss-2, and the resulting phenotype was observed in a plasmid-driven reconstituted rsv rna polymerase system. expression from a genome analogue containing two reporter gen ... | 1999 | 10233981 |
| virus-inducible expression of a host chemokine gene relies on replication-linked mrna stabilization. | analysis of the host response to viral infection generally has focused on the capacity of viruses to activate or repress transcription of cellular genes, and this approach is also characteristic of work on rna viruses such as respiratory syncytial virus (rsv). in the present study, it appeared initially that rsv-driven expression of a critical immune regulator, the beta-chemokine rantes (regulated upon activation, normal t cell expressed and secreted), in primary-culture airway epithelial cells ... | 1999 | 10318944 |
| 2-5a-dna conjugate inhibition of respiratory syncytial virus replication: effects of oligonucleotide structure modifications and rna target site selection. | to define more fully the conditions for 2-5a-antisense inhibition of respiratory syncytial virus (rsv), relationships between 2-5a antisense oligonucleotide structure and the choice of rna target sites to inhibition of rsv replication have been explored. the lead 2-5a-antisense chimera for this study was the previously reported nih8281 that targets the rsv m2 rna. we have confirmed and extended the earlier study by showing that nih8281 inhibited rsv strain a2 replication in a variety of antivira ... | 1999 | 10320045 |
| impact of respiratory syncytial virus immune globulin in 1996-1997: a local controlled comparison. | immune globulin containing high titers of neutralizing antibodies specific for respiratory syncytial virus (rsv) is clinically used to prevent hospitalizations for rsv-related respiratory infections among high-risk infants. however, recommendations regarding which patient populations should receive rsv immune globulin are inconsistent. | 1999 | 10323631 |
| evaluation of a nested reverse transcription-pcr assay based on the nucleoprotein gene for diagnosis of spontaneous and experimental bovine respiratory syncytial virus infections. | the first nested reverse transcription (rt)-pcr based on the nucleoprotein gene (n rt-pcr-n) of the bovine respiratory syncytial virus (brsv) has been developed and optimized for the detection of brsv in bronchoalveolar lavage fluid cells of calves. this test is characterized by a low threshold of detection (0.17 pfu/ml), which is 506 times lower than that obtained by an enzyme immunosorbent assay (eia) test (rsv testpack abbott). during an experimental infection of 17 immunocompetent calves les ... | 1999 | 10325337 |
| effects of humanization by variable domain resurfacing on the antiviral activity of a single-chain antibody against respiratory syncytial virus. | hnk20 is a mouse monoclonal iga that binds to the f glycoprotein of respiratory syncytial virus (rsv) and neutralizes the virus, both in vitro and in vivo. the single-chain antibody fragment (scfv) derived from hnk20 is equally active and has allowed us to assess rapidly the effect of mutations on affinity and antiviral activity. humanization by variable domain resurfacing requires that surface residues not normally found in a human fv be mutated to the expected human amino acid, thereby elimina ... | 1999 | 10325407 |
| absence of lung immunopathology following respiratory syncytial virus (rsv) challenge in mice immunized with a recombinant rsv g protein fragment. | the relative immunopathogenic potential of a recombinant fusion protein incorporating residues 130-230 of respiratory syncytial virus (rsv-a) g protein (bbg2na), formalin-inactivated rsv-a (fi-rsv), and phosphate-buffered saline (pbs) was investigated in mice after immunization and rsv challenge. fi-rsv priming resulted in massive infiltration of b cells and activated cd4(+) and cd8(+) t lymphocytes in mediastinal lymph nodes (mln) and lungs, where eosinophilia and elevated ifn-gamma, il-2, -4, ... | 1999 | 10329575 |
| clinical impact of preoperative respiratory syncytial virus testing. | the purpose of this study was to determine if preoperative assessment (i.e., physical examination and history review) of pediatric patients can predict positive test results for respiratory syncytial virus (rsv) infection, surgery cancellations, and adverse anesthetic outcomes. approximately 400 pediatric patients were identified for the study from infection control statistics and classified by rsv test result and surgery cancellation status into three groups for analyses. researchers analyzed r ... | 1999 | 10332554 |
| a novel and effective intranasal immunization strategy for respiratory syncytial virus. | in designing subunit vaccination strategies for respiratory syncytial virus (rsv), immunization by mucosal routes may present a realistic alternative to parenteral administration for inducing protective immune responses. to this end, we have utilized the balb/c mouse model and an adjuvant formulation containing caprylic/capric glycerides (ccg) and polyoxyethylene-20-sorbitan monolaurate (ps). the intranasal (i.n.) delivery of purified natural f protein (3 microg per vaccine) formulated with ccg- ... | 1999 | 10333241 |
| adenovirus-mediated transgene expression in nonhuman primate brain. | transgene expression in the brain of st. kitts green monkey, cercopithecus aethiops sabeus, was studied following injection of a serotype 5 adenoviral vector deleted in e1 and e3. the vector harbored the transgene for escherichia coli beta-galactosidase (beta-gal) with the simian virus 40 (sv40) nuclear localization signal under control of the rous sarcoma viral (rsv) long terminal repeat. several titers ranging from 5 x 10(7) to 2 x 10(9) plaque-forming units (pfu) in volumes ranging from 5 to ... | 1999 | 10340549 |
| respiratory syncytial virus infects the bonnet monkey, macaca radiata. | the bonnet monkey model of respiratory syncytial virus (rsv) infection may be a useful nonhuman primate model for studying rsv disease in humans because bonnet monkeys can predictably be infected to obtain an orderly sequence of morphologic, cytologic, virologic, serologic, and inflammatory changes related to time of infection. young feral bonnet monkeys, macaca radiata, were infected endotracheally with 10(6) plaque-forming units (pfu) of the long strain of rsv. rsv was recovered from the anima ... | 1999 | 10347274 |
| respiratory syncytical virus-induced chemokine expression in the lower airways: eosinophil recruitment and degranulation. | characterization of chemokine expression patterns in virus-infected epithelial cells provides important clues to the pathophysiology of such infections. the aim of this study was to determine the chemokine response pattern of respiratory epithelium when infected with respiratory syncytial virus (rsv). macrophage inflammatory protein-1-alpha (mip-1-alpha), interleukin-8 (il-8), and rantes concentrations were measured from rsv-infected hep-2, mrc-5, and wi-38 cell culture supernatants daily follow ... | 1999 | 10351940 |
| analysis of ruminant respiratory syncytial virus isolates by rnase protection of the g glycoprotein transcripts. | two different respiratory syncytial virus (rsv) radiolabeled probes were used to characterize the genetic heterogeneity of 25 ruminant rsv isolates by the ribonuclease protection assay. a 32p-radiolabeled antisense rna probe was transcribed from cloned ovine and bovine rsv g glycoprotein genes and then hybridized with total rna isolated from infected cells with various ruminant rsv isolates. the results of this study, along with previously published nucleotide sequence data of the ovine rsv g gl ... | 1999 | 10353351 |
| a direct comparison of the activities of two humanized respiratory syncytial virus monoclonal antibodies: medi-493 and rshzl9. | two humanized monoclonal antibodies, medi-493 and rshz19, were developed independently as potential improvements over rsv-igiv for prevention of respiratory syncytial virus (rsv) infection. rsv-igiv is a polyclonal human antibody preparation for intravenous infusion enriched for rsv neutralizing activity. a phase iii clinical trial showed that medi-493 significantly reduced hospitalizations due to rsv infection. in a separate trial, rshz19 failed to show significant efficacy. in new studies, the ... | 1999 | 10353858 |
| severe respiratory syncytial virus disease in alaska native children. rsv alaska study group. | hospitalization rates for respiratory syncytial virus (rsv) infection range from 1 to 20/1000 infants. to determine the rate and severity of rsv infections requiring hospitalization for infants in the yukon-kuskokwim (yk) delta of alaska, a 3-year prospective surveillance study was conducted. the annual rate of rsv hospitalization for yk delta infants <1 year of age was 53-249/1000. rsv infection was the most frequent cause of infant hospitalization. rsv disease severity did not differ among non ... | 1999 | 10353859 |
| [round table: urticaria in relation to infections]. | 1) to study the clinical and analytic features of infectious disease associated to urticaria in children. 2) to look into the probable etiology of the infectious disease. 3) to determine atopic predisposition and previous urticarial episodes and to rule out the involvement of antibiotics. | 1999 | 10354011 |
| subgroup-a rous sarcoma virus-induced growth stimulation of chick embryos infected via the chorioallantoic membrane. | chicks that hatch from eggs containing group specific antigen (gs antigen) of lymphoid leukosis virus (llv) subgroups, grow poorly. in our laboratory for more precise identification of llv-of subgroup a (llv-a) resistant and susceptible genotypes by progeny testing, the chorioallantoic membrane (cam) assay in complemented by liver tumour (lt) assay, wherein rous sarcoma virus (rsv) of subgroup a (homologous to llv-a) was used. the present study was conducted in a light breed (white leghorn) and ... | 1999 | 10355368 |
| response to wing-web challenge of rous sarcoma virus subgroups in some chicken breeds. | response to wing-web challenge (wwc) of rous sarcoma virus (rsv) subgroups was studied in 4-8 weeks old chicks of a light breed, a heavy breed and a cross between an indigenous black plumage bantam fowl and australorp breed. wing-web tumor (wwt) began to develop within one week in response to virus subgroups a (bs-rsv) and c [rsv (rav-49)] challenge. in chicks challenged with subgroup d [rsv (rav-50)] virus it took a minimum of 4 weeks for development of wwt. positive response to wwc by subgroup ... | 1999 | 10355369 |
| viral infection modulates expression of hypersensitivity pneumonitis. | hypersensitivity pneumonitis (hp) is a granulomatous, inflammatory lung disease caused by inhalation of organic ags, most commonly thermophilic actinomycetes that cause farmer's lung disease. the early response to ag is an increase in neutrophils in the lung, whereas the late response is a typical th1-type granulomatous disease. many patients who develop disease report a recent viral respiratory infection. these studies were undertaken to determine whether viruses can augment the inflammatory re ... | 1999 | 10358192 |
| rapamycin-resistant phosphorylation of the initiation factor-4e-binding protein (4e-bp1) in v-src-transformed hamster fibroblasts. | increased phosphorylation of the translational repressor protein 4e-bp1 was found in the cell line derived from the tumor induced in syrian hamster by rous sarcoma virus (rsv). this was accompanied by its dissociation from the complex with initiation factor eif4e. the ribosomal s6 protein kinase p70s6k is supposed to be regulated by the same or a closely related rapamycin-sensitive signalling pathway to that which modulates 4e-bp1. phosphorylation and activity of p70s6k were found to be also inc ... | 1999 | 10362146 |
| induction of antibody responses in the common mucosal immune system by respiratory syncytical virus immunostimulating complexes. | immunostimulating complexes (iscoms) containing envelope proteins of respiratory syncytial virus (rsv) were explored as a mucosal delivery system for the capacity of inducing a common mucosal antibody response. two intranasal (i.n.) administrations of balb/c mice with iscoms induced potent serum igg, and strong iga responses to rsv locally in the lungs and the upper respiratory, and remotely in the genital and the intestinal tracts. virtually no measurable iga response was found in these mucosal ... | 1999 | 10363675 |
| impact of palivizumab on expected costs of respiratory syncytial virus infection in preterm infants: potential for savings. | in its clinical assessment of the respiratory syncytial virus (rsv)-specific monoclonal antibody palivizumab, the impact-rsv study group demonstrated a reduction in hospitalizations for rsv-related lower respiratory tract infection in infants who received prophylaxis compared with infants who did not receive prophylaxis. an assessment of the rsv-related expenses for managing both groups of infants is needed to provide insight into the value of prophylaxis. the present study was conducted to iden ... | 1999 | 10363740 |
| identification of multiple protective epitopes (protectopes) in the central conserved domain of a prototype human respiratory syncytial virus g protein. | a recombinant fusion protein (bbg2na) comprising the central conserved domain of the respiratory syncytial virus subgroup a (rsv-a) (long) g protein (residues 130 to 230) and an albumin binding domain of streptococcal protein g was shown previously to protect mouse upper (urt) and lower (lrt) respiratory tracts against intranasal rsv challenge (u. f. power, h. plotnicky-gilquin, t. huss, a. robert, m. trudel, s. stahl, m. uhlén, t. n. nguyen, and h. binz, virology 230:155-166, 1997). panels of m ... | 1999 | 10364313 |
| role of the m2-1 transcription antitermination protein of respiratory syncytial virus in sequential transcription. | m2-1 protein of human respiratory syncytial virus (rsv) is a transcription antitermination factor that is important for the efficient synthesis of full-length mrnas as well as for the synthesis of polycistronic readthrough mrnas, which are characteristic of nonsegmented negative-strand rna viruses. the contributions of these effects to rsv sequential transcription were investigated with minigenomes which contained one to five genes which were either foreign marker genes or authentic rsv genes. w ... | 1999 | 10364337 |
| biodegradable alginate microspheres as a delivery system for naked dna. | sodium alginate is a naturally occurring polysaccharide that can easily be polymerized into a solid matrix to form microspheres. these biodegradable microspheres were used to encapsulate plasmid dna containing the bacterial beta-galactosidase (lacz) gene under the control of either the cytomegalovirus (cmv) immediate-early promoter or the rous sarcoma virus (rsv) early promoter. mice inoculated orally with microspheres containing plasmid dna expressed lacz in the intestine, spleen and liver. ino ... | 1999 | 10369574 |
| synergistic effect of immunization with a peptide cocktail inducing antibody, helper and cytotoxic t-cell responses on protection against respiratory syncytial virus. | respiratory syncytial virus (rsv)-specific cytotoxic t lymphocytes (ctl) or neutralizing antibodies can protect against rsv infection when induced separately by immunization with synthetic peptides. in the work described here, rsv-specific neutralizing antibodies and ctls were induced after immunization with a cocktail of peptides consisting of a b-cell mimotope (s1s-map), a t-helper epitope (sh:45-60) and a ctl epitope linked to a fusion (f) peptide (f/m2:81-95) that were comparable to those in ... | 1999 | 10374957 |
| transformation by v-myb. | the v-myb oncogene of the avian myeloblastosis virus (amv) is unique among known oncogenes in that it causes only acute leukemia in animals and transforms only hematopoietic cells in culture. amv was discovered in the 1930s as a virus that caused a disease in chickens that is similar to acute myelogenous leukemia in humans (hall et al., 1941). this avian retrovirus played an important role in the history of cancer research for two reasons. first, amv was used to demonstrate that all oncogenic vi ... | 1999 | 10378700 |
| atrial natriuretic peptide gene delivery attenuates gentamycin-induced nephrotoxicity in rats. | atrial natriuretic peptide (anp) is a cardiac hormone which exerts potent natriuretic and vasorelaxant activities. the aim of this study is to investigate potential protective effects of anp gene delivery in gentamycin-induced nephrotoxicity. | 1999 | 10382996 |
| support plasmids and support proteins required for recovery of recombinant respiratory syncytial virus. | respiratory syncytial virus (rsv) can be recovered from plasmids that separately encode antigenomic rna and the n, p, l, and m2-1 proteins of the nucleocapsid. however, in a recent study the inclusion of a separate m2-1 expression plasmid was found to be unnecessary (h. jin, d. clarke, h. zhou, x. cheng, k. coelingh, m. bryant, and s. li, virology 1998, 251, 206-214). this suggested that the m2-1 protein, which is a transcription antitermination factor, is not required to reconstitute the minimu ... | 1999 | 10388648 |
| efficacy of directigen rsv testing in patient management following admission from a paediatric emergency department. | we investigated the use of the directigen respiratory syncytial virus test performed under 'stat laboratory' conditions, in the management of infants after admission from the paediatric emergency department (ed). the study group consisted of 242 consecutive paediatric ed patients tested by directigen in the stat laboratory during the winter 1995-1996 respiratory virus season. specimens were submitted to the virology laboratory for confirmatory consensus testing utilizing in part, an in-house mul ... | 1999 | 10392339 |
| optimizing vascular gene transfer of plasminogen activator inhibitor 1. | the vessel wall fibrinolytic system plays an important role in maintaining the arterial phenotype and in regulating the arterial response to injury. plasminogen activator inhibitor type 1 (pai-1) regulates tissue fibrinolysis and is expressed in arterial tissue; however, its biological role remains uncertain. to help elucidate the role of pai-1 in the artery wall, and to begin to clarify whether manipulation of vascular pai-1 expression might be a target for gene therapy, we used adenoviral vect ... | 1999 | 10395372 |
| human monoclonal antibodies isolated from spontaneous epstein-barr virus-transformed tumors of hu-spl-scid mice and specific for fusion protein display broad neutralizing activity toward respiratory syncytial virus. | two human monoclonal antibodies, rf-1 and rf-2, specifically recognize the fusion protein of the human respiratory syncytial virus (rsv). these were isolated from spontaneous tumors in scid mice reconstituted with human splenocytes and boosted with fusion protein. the tumors consisted of epstein-barr virus-transformed human b cells in animals with antigen-specific antibody titers>105. the binding affinity of rf-1 and rf-2 to the fusion protein is 1010 and 109 m-1, respectively. the antibodies bi ... | 1999 | 10395839 |
| the gag domains required for avian retroviral rna encapsidation determined by using two independent assays. | the rous sarcoma virus (rsv) gag precursor polyprotein is the only viral protein which is necessary for specific packaging of genomic rna. to map domains within gag which are important for packaging, we constructed a series of gag mutations in conjunction with a protease (pr) active-site point mutation in a full-length viral construct. we found that deletion of either the matrix (ma), the capsid (ca), or the protease (pr) domain did not abrogate packaging, although the ma domain is likely to be ... | 1999 | 10400719 |
| changes in rous sarcoma virus rna secondary structure near the primer binding site upon trnatrp primer annealing. | predicted secondary-structure elements encompassing the primer binding site in the 5' untranslated region of rous sarcoma virus (rsv) rna play an integral role in multiple viral replications steps including reverse transcription, dna integration, and rna packaging (a. aiyar, d. cobrinik, z. ge, h. j. kung, and j. leis, j. virol. 66:2464-2472, 1992; d. cobrinik, a. aiyar, z. ge, m. katzman, h. huang, and j. leis, j. virol. 65:3864-3872, 1991; j. t. miller, z. ge, s. morris, k. das, and j. leis, j ... | 1999 | 10400722 |
| induction of th-1 and th-2 responses by respiratory syncytial virus attachment glycoprotein is epitope and major histocompatibility complex independent. | in balb/c mice, sensitization to respiratory syncytial virus (rsv) attachment (g) glycoprotein leads to the development of lung eosinophilia upon challenge infection with rsv, a pathology indicative of a strong in vivo induction of a th-2-type response. in this study, we found that a strong, rsv g-specific, th-1-type cytokine response occurred simultaneously with a th-2-type response in g-primed mice after rsv challenge. both th-1 and th-2 effector cd4(+) t cells recognized a single immunodomina ... | 1999 | 10400756 |
| identification of a linear heparin binding domain for human respiratory syncytial virus attachment glycoprotein g. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract disease in infants and young children worldwide. infection is mediated, in part, by an initial interaction between attachment protein (g) and a highly sulfated heparin-like glycosaminoglycan (gag) located on the cell surface. synthetic overlapping peptides derived from consensus sequences of the g protein ectodomain from both rsv subgroups a and b were tested by heparin-agarose affinity chromatography for their abi ... | 1999 | 10400758 |
| mass determination of rous sarcoma virus virions by scanning transmission electron microscopy. | the internal structural protein of retroviruses, gag, comprises most of the mass of the virion, and gag itself can give rise to virus-like particles when expressed in appropriate cells. previously the stoichiometry of gag in virions was inferred from indirect measurements carried out 2 decades ago. we now have directly determined the masses of individual particles of the prototypic avian retrovirus, rous sarcoma virus (rsv), by using scanning transmission electron microscopy. in this technique, ... | 1999 | 10400808 |
| viral aetiology of lower respiratory tract infection in young malaysian children. | to study the viral aetiology of lower respiratory tract infection (lrti) in young malaysian children. | 1999 | 10404452 |
| attenuation of the recombinant human parainfluenza virus type 3 cp45 candidate vaccine virus is augmented by importation of the respiratory syncytial virus cpts530 l polymerase mutation. | a phenylalanine to leucine mutation at position 521 in the l polymerase of cpts530, a live-attenuated respiratory syncytial virus (rsv) cold-passaged (cp), temperature-sensitive (ts) candidate vaccine, specifies the ts and attenuation (att) phenotypes. sequence alignment of this region in the l proteins of several distantly related paramyxoviruses revealed that this phenylalanine is conserved. using reverse genetics, the analogous phenylalanine at position 456 in the l protein of wild-type piv3 ... | 1999 | 10405364 |
| macrophage inflammatory protein-1alpha and rantes are present in nasal secretions during ongoing upper respiratory tract infection. | macrophage inflammatory protein-1alpha (mip-1alpha) and rantes (regulated upon activation, normal t-cell expressed and secreted) were measured by enzyme-linked immunosorbent assay (elisa) from virus-infected respiratory cell culture supernatants and from 100 nasal wash samples obtained from patients aged 8 d to 10 yr. the results of the nasal wash samples were analyzed in relation to the etiology of the viral infection. in vitro, respiratory syncytial virus (rsv) induced the production of mip-1a ... | 1999 | 10410916 |
| involvement of caveolae in the uptake of respiratory syncytial virus antigen by dendritic cells. | the uptake of respiratory syncytial virus (rsv) antigen by cattle dendritic cells was investigated. pathways of antigen uptake were monitored by flow cytometry using specific tracers and by proliferation assays, which were used to measure the presentation of rsv antigen and ovalbumin. inhibitors that differentially affected pathways were used to distinguish them. presentation of rsv antigen, but not ovalbumin, was inhibited by phorbol myristate acetate and filipin, which have been reported to in ... | 1999 | 10410989 |
| respiratory syncytial virus infection of human respiratory epithelial cells enhances inducible nitric oxide synthase gene expression. | the induction kinetics of the mrna of interferon regulatory factor 1 (irf-1), inducible nitric oxide synthase (inos), and proinflammatory cytokines in respiratory syncytial virus (rsv)-infected human type 2 alveolar epithelial cells (a549 cells) were analyzed semiquantitatively by rt-pcr. rsv enhanced irf-1 and inos mrna expression as early as 4 h after rsv infection and this enhancement lasted several hours. no ifn-gamma gene expression was observed during the whole course of the infection. exp ... | 1999 | 10410996 |
| adenoviral nerve growth factor and beta-galactosidase transfer to spinal cord: a behavioral and histological analysis. | the present study characterizes the time course and loci of gene expression induced by the administration of adenoviral vectors into spinal cord. although a marked inflammatory response to these vectors occurred, no effect on spinal cord function was seen in the 1st postoperative week. the expression of transgenic genes delivered by viral vectors is being exploited throughout the nervous system. the present study utilized adenoviral vectors containing the rous sarcoma virus (rsv) promoter and a ... | 1999 | 10413133 |
| surfactant replacement therapy in rsv-induced acute respiratory distress syndrome (ards). | acute respiratory distress syndrome (ards) associated with severe respiratory syncytial virus infection is rare. we report a 5-month-old indian girl who was admitted to our intensive care ward with severe respiratory failure who fulfilled the criteria for ards using both murray's lung injury score of > 2.5 and the american-european consensus conference definition for ards. she developed diffuse bilateral alveolar infiltrates, severe hypoxaemia (pao2/fio2 < 100) and required high peep (> 15 cm h2 ... | 1999 | 10414173 |
| clinical and epidemiological aspects of respiratory syncytial virus lower respiratory tract infections. | this paper has analyzed respiratory syncytial virus lower respiratory tract infections in 201 hospitalized children. in children with wheezing, erythrocyte sedimentation rate (esr) was significantly higher in those with pneumonia than with syndroma pertussis, while the white blood cell (wbc) count was significantly lower in patients with bronchitis than in those with bronchiolitis and syndroma pertussis. bronchodilatators were applied in 75.6% and corticosteroids in 20% of patients. ten patients ... | 1999 | 10414377 |
| effect of respiratory syncytial virus on subsequent allergic sensitization to ovalbumin in guinea-pigs. | children with acute respiratory syncytial virus (rsv) bronchiolitis often develop recurrent wheezing, asthma and allergic sensitization, but the role of rsv in the pathogenesis of these sequelae is unclear. this study examined whether rsv infection potentiates subsequent allergic sensitization, airway hyperresponsiveness (ahr) and airway inflammation induced by repeated exposures to aerosolized ovalbumin (oa) in guinea-pigs. guinea-pigs received either rsv or sham inoculum, followed by exposures ... | 1999 | 10414392 |
| respiratory system mechanics in patients receiving aerosolized ribavirin during mechanical ventilation for suspected respiratory syncytial viral infection. | respiratory syncytial virus (rsv) is an important respiratory pathogen for infants. aerosolized ribavirin (ar) has been used in mechanically ventilated (mv) patients with rsv bronchiolitis. the purpose of this study was to measure respiratory system mechanics (rsm) in pediatric patients requiring mv and receiving ar for suspected rsv. patients were prospectively randomized to receive ar, either at a regular dose (rd) (6 g/300 ml over 18 hr/day) or a high dose (hd) (6 g/100 ml over 2 hr, three ti ... | 1999 | 10423311 |
| phosphorylation of p125fak and paxillin focal adhesion proteins in src-transformed cells with different metastatic capacity. | hamster fibroblasts transformed by rous sarcoma virus (rsv) display different metastatic potentials that are associated with specific structural features of the v-src oncoprotein. this diverse metastatic activity could be due to various tyrosine phosphorylation levels of specific src protein substrates. to check this hypothesis, phosphorylation of the fak and paxillin proteins, involved in signal transduction pathways and known as src protein substrates, was tested. it was shown that fak and pax ... | 1999 | 10428489 |
| [coincidental outbreaks of rotavirus and respiratory syncytial virus in paris: a survey from 1993 to 1998]. | in a pediatric hospital of paris, from 1993 to 1998, respiratory secretions were positive for respiratory syncytial virus (rsv) in 26.3% of 4,738 children (0-5 years) examined or hospitalized for lower respiratory tract infections. rotavirus detection was positive in stools of 23.7% of the 8,537 children of the same age with acute diarrhea. | 1999 | 10429813 |
| respiratory syncytial virus: a continuing culprit and conundrum. | for more than 4 decades, respiratory syncytial virus (rsv) has been recognized as a worldwide pathogen of import. in the united states alone, approximately 91,000 infants are hospitalized with rsv infections yearly, at an estimated annual cost of at least 300 million dollars. the burden of rsv infections is even greater if outpatient visits for children and adults and rsv morbidity in patients with underlying conditions are included. obstacles to controlling rsv remain appreciable and challengin ... | 1999 | 10431132 |
| pulmonary function changes in children after respiratory syncytial virus infection in infancy. | during the last 20 years, an association between respiratory syncytial virus (rsv) bronchiolitis or pneumonia in infants and abnormal pulmonary function later in childhood has been established. study designs have varied considerably, but most investigators have used an observational approach in which children with early bronchiolitis or pneumonia are identified and pulmonary function is measured later in childhood. decreased forced expiratory flows at mid-lung volumes and increased airway reacti ... | 1999 | 10431136 |