Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| effects of trichomonas vaginalis on the ph and glycogen content of the vagina. | 1985 | 4054105 | |
| alterations of cervical cytology and steroid contraceptive use. | the alterations of cervical cytology in 3206 'users' of steroid contraceptives (sc) and 2394 'non-users' (controls) have been followed by means of five surveys for eight years. the alterations of cervical cytology have not differed significantly between the two groups seven years after the first examination, but have differed significantly between younger and older women in both groups. the incidence of progression to and regression from pap iii class cytology was more frequent than the prevalen ... | 1985 | 4055204 |
| [occurrence of trichomonas vaginalis infection in patients with neoplasms of the female genitalia]. | 1985 | 3834693 | |
| [a patient with urogenital trichomoniasis unsuccessfully treated due to resistance of the causative agent to nitroimidazole preparations]. | 1985 | 3871654 | |
| effect of culture medium iron content on the biochemical composition and metabolism of trichomonas vaginalis. | trichomonas vaginalis grown in iron-enriched medium contained increased concentrations of iron-sulfur proteins, including ferredoxin and pyruvate-ferredoxin oxidoreductase. the increases in hydrogenosomal constituents correlated with increased in vivo hydrogenosomal metabolism. | 1985 | 3871763 |
| "in vitro" effect of microtubule inhibitors on trichomonas vaginalis. | this paper describes the effects of some antimicrotubular drugs (mebendazole, flubendazole, thiabendazole, colchicine, griseofulvin, vinblastine and isopropyl-n-phenylcarbamate or ipc) on growth and viability of trichomonas vaginalis. among the inhibitors tested, mebendazole and flubendazole irreversibly inhibit protozoa growth at low concentration (greater than or equal to 1 microgram/ml), while colchicine and griseofulvin act at higher concentrations and thiabendazole and ipc are ineffective. ... | 1985 | 3871893 |
| sensitivity testing of trichomonas vaginalis using bromocresol purple indicator. | 1985 | 3872359 | |
| reduction of nitroimidazole derivatives by hydrogenosomal extracts of trichomonas vaginalis. | the reduction rate of nitroimidazole derivatives by pyruvate:ferredoxin oxidoreductase activity in ferredoxin depleted hydrogenosomal extracts of trichomonas vaginalis depended on the one-electron midpoint potential (e7(1)) of 15 compounds out of the 16 tested. the results showed a linear correlation with a positive slope between the logarithm of the rate and the e7(1) in the range from -564 to -260 mv. addition of t. vaginalis ferredoxin stimulated the reduction. the additional rate (stimulated ... | 1985 | 3872412 |
| in vitro inhibition of giardia lamblia and trichomonas vaginalis growth by bithionol, dichlorophene, and hexachlorophene. | bithionol, dichlorophene, and hexachlorophene, which are used in treating some helminthic infections, killed trophozoites of giardia lamblia and trichomonas vaginalis in modified bi-s-33 and asami media, respectively. virtually all g. lamblia and t. vaginalis cells were killed within 24 h with a 0.42 mm concentration of these compounds, except that 0.93 mm dichlorophene was required for sterilizing t. vaginalis in the same period. in modified bi-s-33 and asami media from which bovine and human s ... | 1985 | 3872626 |
| measurement of adenine uptake as an in vitro screen for antitrichomonal agents. | a semi-automated microdilution test was adapted for the in vitro testing of potential antitrichomonal agents. washed cell suspensions of the pathogenic protozoan trichomonas vaginalis were incubated in microtitration plates for 4 h at 37 degrees c in the presence radiolabelled adenine and serial dilutions of the test compounds. after automatic harvesting of the cells onto filter paper discs, the extent of adenine uptake was estimated by scintillation counting. from the dose-response data it was ... | 1985 | 3872780 |
| isolation and characterization of dna from tritrichomonas foetus and trichomonas vaginalis. | high molecular weight dna samples free of contaminating proteins or rna were obtained from tritrichomonas foetus or trichomonas vaginalis by lysing the cells in 4 m guanidinium thiocyanate before centrifuging in cscl density gradient and then purifying the dna band by nacs-37 column chromatography. the bulk dna from either organism acted as a single component in ion-exchange chromatography, agarose gel electrophoresis, cscl density gradient centrifugation and thermal denaturation. t. foetus dna ... | 1985 | 3873006 |
| [trichomoniasis of the urogenital tract in patients hospitalized in 1976-1983]. | we report on investigations carried out on t. vaginalis and mixed infection (t. vaginalis and yeast-like fungi). special attention was paid to metronidazol resistance on t. vaginalis strains isolated from patients who had been repeatedly treated with this medicament. therefore we point to the necessity of defining the patients' susceptibility to this remedy. | 1985 | 3873142 |
| use of a time-kill technique for susceptibility testing of trichomonas vaginalis. | the emergence of metronidazole-resistant trichomonas vaginalis and questions about the safety of metronidazole are significant concerns in treatment of trichomoniasis. at 24 h, a microtiter assay was used to test antimicrobial susceptibility of 16 recent isolates; the mics of metronidazole ranged from less than 0.06 to 25 micrograms/ml. observable motility as an endpoint correlated imperfectly with survival as measured in pour plates. quantitative pour plate cultures of six t. vaginalis isolates ... | 1985 | 3873214 |
| prevalence of low genital tract infectious agents in young israeli women. | low genital tract infectious agents were examined 411 times in 386 young women. each examination included anamnesis, physical examination and broad-spectrum microbiologic analysis for the detection of: ureaplasma urealyticum, chlamydia trachomatis, trichomonas vaginalis, candida species, mycoplasma hominis, streptococcus beta hemolyticus group b, hemophilus vaginalis, neisseria gonorrhoeae and syphilis serology. three hundred and six patients were symptomatic for low genital tract infection and ... | 1985 | 3873446 |
| proliferative response of human lymphocytes to secretory and cellular antigens of trichomonas vaginalis. | lymphocytes from patients with active trichomoniasis showed a proliferative response when incubated in the presence of secretory and cellular products of either pathogenic or non-pathogenic trichomonas vaginalis. maximal responses were detected using 50 micrograms per ml protein after 5 days incubation. lymphocytes from non-infected laboratory workers showed no response to these antigens. this indicates that delayed hypersensitivity reactions may act to modulate inflammatory responses in trichom ... | 1985 | 3874274 |
| ultrastructural changes of trichomonas vaginalis prior and after cryopreservation. | fine structural changes of trichomonas vaginalis are described prior and after the freezing process in liquid nitrogen. dimethyl sulfoxide (dmso) used as the cryoprotectant caused distinct alterations of the cytoplasm when trichomonads were equilibrated with 5% dmso under various experimental conditions. changes were bubble-like protrusions, fissuration and/or vacuolation of the cytoplasm, doubling and removal or/and rupture of the cell membrane. apart from these findings cryopreservation caused ... | 1985 | 3874499 |
| trichomonas vaginalis infestation in sexually abused girls. | we report infection with trichomonas vaginalis in four sexually abused girls; two of these patients are unique in that they were premenarchal. we discuss the clinical features in premenarchal and postmenarchal children and relate infection to the likelihood of prior sexual abuse. | 1985 | 3875279 |
| importance of differential diagnosis in acute vaginitis. | acute vaginitis is one of the most common diseases seen in the practice of office gynecology. large survey studies of women with lower genital tract symptoms suggestive of vaginitis have demonstrated the presence of three major etiologic categories in acute vaginitis: (1) nonspecific vaginosis (gardnerella vaginalis), (2) vulvovaginal candidiasis (candida albicans), and (3) trichomoniasis (trichomonas vaginalis). effective treatment of acute vaginitis requires that an accurate diagnosis be estab ... | 1985 | 3875290 |
| 13c-nmr reveals glycerol as an unexpected major metabolite of the protozoan parasite trichomonas vaginalis. | 13c-nmr has been used to study the kinetics of the formation of metabolites from [1-13c]glucose in intact cells of trichomonas vaginalis during anaerobic incubation. as well as the expected metabolites lactate and acetate, this technique revealed glycerol as an additional major product, present in amounts equimolar with acetate. the formation of glycerol is readily explained in terms of the need to maintain redox balance. this protozoan now joins the small group of organisms which are known to p ... | 1985 | 3876951 |
| vitamin b12 requirement for the growth of trichomonas vaginalis in vitro. | 1985 | 3877156 | |
| specific nature of trichomonas vaginalis parasitism of host cell surfaces. | the adherence of trichomonas vaginalis nyh 286 to host cells was evaluated by using monolayer cultures of hela and hep-2 epithelial cells and human fibroblast cell lines. saturation of sites on hela cells was achieved, yielding a maximal t. vaginalis nyh 286-to-cell ratio of two. the ability of radiolabeled nyh 286 to compete with unlabeled trichomonads for attachment and the time, temperature, and ph-dependent nature of host cell parasitism reinforced the idea of specific parasite-cell associat ... | 1985 | 3877690 |
| contact-dependent cytopathogenic mechanisms of trichomonas vaginalis. | the cytopathogenic mechanisms of trichomonas vaginalis have been debated since the 1940s. we examined the following three proposed pathogenic mechanisms: contact-dependent extracellular killing, cytophagocytosis, and extracellular cytotoxins. serial observations of chinese hamster ovary (cho) cell monolayers exposed to trichomonads revealed that (i) trichomonads form clumps, (ii) the clumps adhere to cells in culture, and (iii) monolayer destruction occurs only in areas of contact with t. vagina ... | 1985 | 3877693 |
| microbiology of the lower genital tract in postmenarchal adolescent girls: differences by sexual activity, contraception, and presence of nonspecific vaginitis. | the prevalence of selected microorganisms in the lower genital tract in postmenarchal adolescent girls was assessed, including vaginal gardnerella vaginalis, group b streptococcus, lactobacillus, mycoplasma species, ureaplasma urealyticum, staphylococcus aureus, and yeast, and endocervical mycoplasma species, u. urealyticum, chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis. specific attention was focused on important sexually transmitted disease organisms, and differences ... | 1985 | 3877803 |
| [specific antibodies in women treated for trichomonas vaginitis]. | 1985 | 3878629 | |
| [methods of obtaining a pure culture of trichomonas vaginalis]. | 1985 | 3879061 | |
| [vaginal microbial flora in a normal term pregnancy]. | 1985 | 3879109 | |
| aspartate: 2-oxoglutarate aminotransferase from trichomonas vaginalis. identity of aspartate aminotransferase and aromatic amino acid aminotransferase. | aspartate: 2-oxoglutarate aminotransferase from the anaerobic protozoon trichomonas vaginalis was purified to homogeneity and characterized. it is a dimeric protein of overall mr approx. 100000. only a single isoenzyme was found in t. vaginalis. the overall molecular and catalytic properties have features in common with both the vertebrate cytoplasmic and mitochondrial isoenzymes. the purified aspartate aminotransferase from t. vaginalis showed very high rates of activity with aromatic amino aci ... | 1985 | 3879173 |
| isolation of gardnerella vaginalis from high vaginal swabs. | vaginal swabs from 2715 consecutive unselected women with vaginal discharge were examined for gardnerella vaginalis, candida albicans and trichomonas vaginalis. of 2715 women, 2043 had non-specific vaginitis and g. vaginalis was isolated from 269 cases (13.2%). thirty five of the strains were isolated together with c. albicans, eight with t. vaginalis, six with bacteroides species and three each with group b streptococcus and staphylococcus aureus. two hundred and sixteen (80%) of the isolates w ... | 1985 | 3879398 |
| [ultrastructural study of the in vitro mode of action of p-chlorophenyl bis-(2-hydroxynaphthyl)methane on trichomonas vaginalis]. | 1985 | 3879716 | |
| [antimicrobial activity of nifuratel]. | 1985 | 3879754 | |
| [antimicrobial activity of a douche containing ketoconazole]. | 1985 | 3879755 | |
| [effect of some steroid hormones on the growth of trichomonas vaginalis]. | studies were conducted to determine the effect of some steroid hormones, particularly sex hormones, on the growth of trichomonas vaginalis "in vitro". 17 alpha-estradiol and 17 beta-estradiol were stimulatory for parasite growth, progesterone and testosterone were inhibiting, whereas cholesterol and ergosterol lacked such effects. changes on the growth of t. vaginalis were observed in the first 20 hours of incubation with specific hormonal concentrations. these observations confirm the influence ... | 1985 | 3879810 |
| [detection of specific antibodies against trichomonas vaginalis in human serum]. | 1985 | 3882195 | |
| the treatment of trichomonas vaginalis vaginitis. an open controlled prospective study comparing a single dose of metronidazole tablets, benzoyl metronidazole suspension and tinidazole tablets. | metronidazole tablets, benzoyl metronidazole suspension and tinidazole tablets each given as a single 2 g bolus dose in an open controlled prospective study were found to be effective forms of treatment for trichomonas vaginalis vaginitis. the cure rates on wet smear preparations were 100%, 100% and 94,8% respectively, and symptomatic and observed improvements were excellent. there was a statistically significant difference between the response rates associated with both metronidazole preparatio ... | 1985 | 3885425 |
| detection and characterization of serum antitrichomonal antibodies in urogenital trichomoniasis. | antibodies against trichomonas vaginalis were detected in serum samples from 98 patients by three immunological assays. a good correlation was observed between the enzyme-linked immunosorbent assay and the immunofluorescence method, whereas it was found that the enzyme-linked immunosorbent assay correlated better with the current or past detection of organisms than did other serological methods (immunofluorescence and hemagglutination). similar results were obtained with whole trichomonads and t ... | 1985 | 3886694 |
| [the trichomonads]. | 1985 | 3887632 | |
| [transmission of urogenital trichomoniasis in female prostitutes]. | a group of female prostitutes (n = 41) was found to be infected with trichomonas vaginalis to 36.6%, diagnosed by using culture methods. the examination of these women for t. vaginalis and their treatment is not obligatory, therefore the transmission of these parasites by infected prostitutes is not properly controlled; transmission of the trichomoniasis continues under nearly optimal conditions. it is urgently necessary to examine female prostitutes for t. vaginalis infections without exception ... | 1985 | 3888822 |
| [clinico-experimental study of local humoral immunity of the vagina in human cases of trichomoniasis]. | 1985 | 3890111 | |
| the modes of action of some anti-protozoal drugs. | 1985 | 3892438 | |
| identification of a surface antigen of trichomonas vaginalis. | a major surface antigen of trichomonas vaginalis was purified by using three independently derived monoclonal antibodies (two immunoglobulin m and one immunoglobulin g1) prepared against t. vaginalis phs-2j. a 115,000-molecular-weight antigen and one or more components with a molecular weight of 58,000 to 64,000 were recovered when any of the three antibodies was used as an immunoadsorbent. the purified antigen reacted with all three monoclonal antibodies in an enzyme-linked immunosorbent assay, ... | 1985 | 3894230 |
| problems in the cultivation of cervical bacteria. | multiple variations in the types, species and prevalence of cervical bacteria are described in the literature. a review of the literature was undertaken to determine the effect of delay on the ability to identify bacteria. experiments were initiated to elaborate on those observations. the rate of organism isolation was related to the delay in transport to the laboratory. | 1985 | 3894658 |
| problems in specimen collection for sexually transmitted diseases. | laboratory methods for the diagnosis of sexually transmitted diseases (stds) are continuously undergoing improvement. it remains the responsibility of the clinician to become familiar with the tests available for the diagnosis of stds. those tests depend on obtaining clinical specimens from the proper site and on transporting them to the laboratory under satisfactory conditions. | 1985 | 3894659 |
| relationship of vaginitis to the sex of conceptuses. | the relative viability of x-bearing and y-bearing spermatozoa is influenced by factors in the vagina such as ph. the vaginal environment, in turn, is influenced by its flora. this study examined the relationship of the vaginal flora to the sex of conceptuses. it was found that women who carried trichomonas vaginalis or bacteroides sp or who had nonspecific vaginitis at first prenatal visit were significantly more likely to deliver females than women who carried none of these organisms (54 versus ... | 1985 | 3895073 |
| electron microscope observations on the interaction of mycoplasma fermentans with trichomonas vaginalis. | cultures of trichomonas vaginalis were found to be contaminated with mycoplasma fermentans. by means of electron microscopy the interaction between the prokaryotic organisms and the trichomonads was examined. cells of m. fermentans were observed in the medium; some of them were attached to the surface of the trichomonads and others were observed in membrane-bounded vacuoles of trichomonads. they were also present in the ground substance of the cytoplasm. the mycoplasmas divided by binary fission ... | 1985 | 3895766 |
| [trichomoniasis]. | 1985 | 3902591 | |
| treatment of the gardnerella vaginalis syndrome. a controlled, double-blind study comparing pivampicillin and metronidazole. | a double-blind, randomized, therapeutic study was carried out in 289 patients with vaginal discharge and growth of gardnerella vaginalis (gv) and no growth of neisseria gonorrhoeae, trichomonas vaginalis or candida. treatment consisted of either pivampicillin (pondocillin), 700 mg twice daily for seven days, or metronidazole, 500 mg twice daily for seven days. the effect was evaluated on the basis of the patient's statement and on the result of culture for gv immediately after end of treatment. ... | 1985 | 3903906 |
| [detection of gardnerella vaginalis in the pathogen spectrum of sexually transmissible diseases in vulvovaginitis]. | gardnerella vaginalis, a sexually transmittable organism, is regarded as the indicator of the so-called "non-specific vaginitis". the isolation rate of g. vaginalis from 72 women attending our out-patient department during one year because of urogenital complaints was higher in pretreated than in untreated patients. in genital swabs taken from untreated patients, g. vaginalis could be isolated only in 17%, whereas this was observed in 38% of the specimens from women pretreated with various antim ... | 1985 | 3907172 |
| [analysis of growth curves of trichomonas vaginalis in the presence of staphylococcus aureus and escherichia coli]. | 1985 | 3909648 | |
| the relation between vaginal ph and the microbiological status in vaginitis. | the vaginal ph, microbial flora and presence of clue cells were investigated in 89 women who were seen at a sexually transmitted diseases clinic with a vaginal discharge or because they were contacts of men with gonococcal or non-gonococcal urethritis or because they were seeking a routine examination. none of the women had received antibiotics for at least 4 weeks before examination. a clinically normal vaginal secretion was found in 21 (55%) of 38 women who had a vaginal ph of 5.0-5.5, while s ... | 1985 | 3910080 |
| [vaginal microscopic evidence in women with symptoms of genital infection]. | 1985 | 3915347 | |
| metronidazole inhibition of hydrogen production in vivo in drug-sensitive and resistant strains of trichomonas vaginalis. | h2 production by the human protozoan parasite trichomonas vaginalis was monitored continuously under a mobile gas phase using a membrane-inlet mass spectrometer. simultaneous and continuous measurement of dissolved h2, o2 and co2 indicated that h2 evolution was inhibited at levels of o2 (less than 0.25 microm) undetectable by the technique, whereas co2 production was stimulated. respiration was not stimulated by admitting h2 to the gas phase. metronidazole inhibited both h2 and co2 production. v ... | 1985 | 3921658 |
| further studies on the surface saccharides in trichomonas vaginalis strains by fluorescein-conjugated lectins. | fluorescence emitted by individual cells of several trichomonas vaginalis strains, nearly all of which were cloned, incubated with fluorescein-conjugated lectins in the absence (experimental) or presence (control) of inhibitory sugars, or else in phosphate-buffered saline alone (autofluorescence) was measured with a leitz mpv compact microfluorometer. irrespective of whether the organisms were postfixed in formalin or glutaraldehyde, the relative fluorescence emitted by the cells was closely com ... | 1985 | 3927600 |
| [gas chromatographic-mass spectrometric analysis of gases produced by trichomonas vaginalis in vitro]. | five strains of trichomonas vaginalis were independently inoculated into cysteine-peptone-liver infusion-maltose medium (cplm medium), and gases in the culture bottle were qualitatively analysed at five days after the inoculation by gas chromatography mass spectrometry (gc-ms). methane, ethane and carbon dioxide were commonly found in the gases produced during the culture of trichomonas vaginalis in vitro. carbon monoxide was also found in the gases from 4 of 5 strains. small amount of both ethy ... | 1985 | 3928776 |
| sexually transmitted diseases in females in a juvenile detention center. | of 37 female adolescent prostitutes and 63 other females held in the king county, washington, juvenile detention facility, 81 (81%) complained of vaginal discharge, and only eight (8%) had no genitourinary symptoms. the prevalence of genital infections among prostitutes and nonprostitutes was similar. neisseria gonorrhoeae was found in 18 (18%) of 98 detainees tested, and chlamydia trachomatis was found in 17 (20%) of 86 detainees tested; either organism was found in 27 (32%) of 85 tested for bo ... | 1985 | 3929406 |
| s-adenosylhomocysteine hydrolase activity in trichomonas vaginalis and other trichomonads. | s-adenosylhomocysteine hydrolase has been detected in crude homogenates of trichomonas vaginalis, tritrichomonas foetus and trichomitus batrachorum at activities of 14, 1.2 and 3.3 nmol min-1 mg-1 protein, respectively. the enzyme from t. vaginalis was found to be soluble with ph optimum of 8.0 and apparent km values for adenosine and homocysteine of 100 and 155 microm, respectively. ara a was shown to inhibit the t. vaginalis enzyme but only at relatively high concentration (i50 100 microm), wh ... | 1985 | 3932851 |
| [prevalence of 7 microorganisms in abnormal vaginal secretions (vaginitis)]. | seven microorganisms (n. gonorrhoeae, c. albicans, t. vaginalis, g. vaginalis, m. hominis, u. urealyticum and streptococcus of group b) have been assayed in genital samplings of 164 asymptomatic women (control group) and of 374 women suffering from abnormal vaginal secretions (vaginitis group). all these bacteria except group b streptococci were isolated in the vaginitis group more frequently than in the control group (p less than 0.01). the proportion of negative cultures for all tested bacteri ... | 1985 | 3934750 |
| influence of a constant magnetic field on antibody formation in experimentally immunized white rats. | 1985 | 3938764 | |
| energy-dispersive x-ray microanalysis of membrane-associated inclusions in hydrogenosomes isolated from trichomonas vaginalis. | energy-dispersive x-ray microprobe analysis of electron-dense inclusions in hydrogenosomes isolated from the aerotolerant anaerobic protozoal human parasite trichomonas vaginalis, bushby strain, indicated the presence of high levels of mg, p and ca. this suggested that divalent cation (e.g. ca2+ or mg2+) accumulation by hydrogenosomes may be important in the regulation of intracellular ion concentrations. | 1985 | 3005473 |
| [vaginitis due to gardnerella vaginalis in a university medical service]. | between august 1983 and june 1984, a total of 363 women were studied who had been referred to the medical service of the school of bacteriology and laboratory clinic of the university of antioquia, medellin, colombia, for obtaining vaginal cytology. they either had or did not have vaginal discharge. the smears were tested for gardnerella vaginalis and trichomonas vaginalis. the majority of the patients were in the third, fourth, and fifth decades of their lives: 51%, 24.5%, and 14%, respectiv ... | 1985 | 12292701 |
| [frequency of trichomonas vaginalis infection in apparently healthy women in western santiago]. | the incidence of infection by trichomonas vaginalis in apparently healthy women was surveyed in the western area of santiago, chile, which comprised both urban and rural areas with medium- or low-income people. a retrospective review of the data of the cytopathology laboratory of san juan de dios hospital was conducted. the data contained all annual pap smears and epidemiological information about the presenting patients. during 1983, a total of 29,501 women had pap smears taken to detect sig ... | 1985 | 12320978 |
| the vaginal contraceptive pill. | vaginal administration of oral contraceptives (ocs) has been shown to be an acceptable and effective method of contraception. the most widely tested vaginal contraceptive pill (vcp) contains 500 mcg of d-1 norgestrel and 50 mcg of ethinyl estradiol (ee) and is administered on the same 21-day schedule as the oral contraceptive. other combination ocs used vaginally include 1 mg of norethisterone plus 50 mcg of mestranol, 250 mcg of levonorgestrel plus 50 mcg of ee, and 2 mg of cyproterone acetat ... | 1985 | 12339969 |
| [vaginal microscopic evidence in women with symptoms of genital infection]. | vaginal living organisms have been defined as the most complete in human ecology. as many as 100 million colonies of microorganisms can live in a single ml of vaginal liquid. more common are the anaerobic types, particularly the lactobacillus and bacteroides. the low ph of the vagina, normally 4, is due to the high lactic acid content of the lactobacillus. pathogenic bacteria is inhibited at 4.5 ph. conversely, it proliferates at a high ph. the slightest alteration of this delicate equilib ... | 1985 | 12341331 |
| trachomoniasis in imo state, nigeria: a first report. | the prevalence of trichomonas vaginalis infection in imo state of nigeria is reported and adds to the meagre knowledge on its distribution in the country. the study (august 1978-march 1981) is based on 794 high vaginal swabs (hvs), 178 male urethral swabs (mus) and 5883 male and female urine samples obtained from out-patients at the owerri general hospital as well as 447 hvs from adult out-patients from holy rosary hospital, emekuku. 10.2% of 1241 hvs, 3.4% of 178 mus and 1.3% of 5883 urine sa ... | 1984 | 12340185 |
| iron uptake and increased intracellular enzyme activity follow host lactoferrin binding by trichomonas vaginalis receptors. | lactoferrin acquisition and iron uptake by pathogenic trichomonas vaginalis was examined. saturation binding kinetics were obtained for trichomonads using increasing amounts of radioiodinated lactoferrin, while no significant binding by transferrin under similar conditions was achieved. only unlabeled lactoferrin successfully and stoichiometrically competed with 125i-labeled lactoferrin binding. time course studies showed maximal lactoferrin binding by 30 min at 37 degrees c. data suggest no int ... | 1984 | 6088662 |
| the deoxyribonucleoside phosphotransferase of trichomonas vaginalis. a potential target for anti-trichomonial chemotherapy. | trichomonas vaginalis, a human protozoan parasite known to lack the capability of synthesizing purine and pyrimidine nucleotides de novo, was found also incapable of converting its ribonucleotides to deoxyribonucleotides. the only apparent means of providing deoxyribonucleotides for dna synthesis relies on salvaging exogenous deoxyribonucleosides by a deoxyribonucleoside phosphotransferase activity in the t. vaginalis 10(5) g pelletable fraction. the activity, constituted by at least two isozyme ... | 1984 | 6090576 |
| sexually transmitted diseases among randomly selected attenders at an antenatal clinic in the gambia. | one hundred randomly selected women attending a free government antenatal clinic in the town of bakau, the gambia, were examined. vaginal swabs were taken for microscopical examination for trichomonas vaginalis and for culture on sabouraud's medium. cervical swabs were taken for culture of neisseria gonorrhoeae and chlamydia trachomatis and, in 50 cases, herpesvirus hominis; in addition, urethral swabs were taken for culture of n gonorrhoeae. serum samples were tested for antibodies to treponema ... | 1984 | 6091843 |
| thiol groups on the surface of anaerobic parasitic protozoa. | evidence is presented that giardia lamblia and entamoeba histolytica, phylogenetically unrelated aerotolerant anaerobes, have crucial thiol groups on or easily accessible to their external surface. both parasites were killed by three structurally unrelated thiol-blocking reagents which penetrate intact cells poorly or not at all. the parasites were protected from p-chloromercuribenzenesulfonic acid (10-100 microm) by cysteine or by reduced glutathione. killing was arrested with identical kinetic ... | 1984 | 6096710 |
| non-gonococcal urethritis in men & its response to therapy. | 1984 | 6097537 | |
| [microbiologic findings in vaginal discharges]. | microbiological examinations were performed in 247 women complaining of vaginal discharge. an average of 3.9 different organisms was isolated from each patient. gardnerella vaginalis was found in 62.8% of all women. candida spp. were seen in 22.7%. trichomonas vaginalis occurred in 6.5%, neisseria gonorrhoeae in 1.2%, chlamydia trachomatis in 10.1%, mycoplasmata in 13.8%, cytomegalovirus in 2.8%, and herpes simplex type 2-virus in 1.1%. none of the above was found in 17.4% of the patients. stati ... | 1984 | 6098512 |
| clinical and microbiological characterization of patients with nonspecific vaginosis associated with motile, curved anaerobic rods. | the vaginal secretions of 20 normal control subjects and 21 patients with motile, curved anaerobic rods were cultured for aerobic and anaerobic bacteria, chlamydia trachomatis, herpes simplex virus, and trichomonas vaginalis. extensive histories and physical examinations of the patients and microscopic appearance and gas-liquid chromatography patterns of vaginal secretions were compared between the two groups. the patients who had motile rods in their vaginal secretions more frequently presented ... | 1984 | 6144713 |
| prevalence of six sexually transmitted disease agents among pregnant inner-city adolescents and pregnancy outcome. | 115 pregnant girls aged 13-17 years were investigated during the third trimester for endocervical infection with six sexually transmissible microorganisms. specimens from 21 patients destroyed the tissue cell monolayers for propagation of chlamydia trachomatis, but 11 were no longer toxic when recultured after freezing or with additional antimicrobial agents; trichomonas vaginalis was present in 76% of the toxic specimens. c trachomatis was recovered from 37% of 105 specimens. t vaginalis was re ... | 1984 | 6146874 |
| salvage of pyrimidine nucleosides by trichomonas vaginalis. | trichomonas vaginalis is incapable of de novo pyrimidine biosynthesis because it cannot incorporate bicarbonate, aspartate or orotate into its pyrimidine nucleotides or nucleic acids. the organism can salvage exogenous cytidine greater than uridine greater than uracil and thymidine, and incorporate them into the nucleotide pool. a portion of cytidine is converted to cmp, cdp and ctp by cytidine phosphotransferase and nucleotide kinases. some cytidine and most of uracil are, however, converted fi ... | 1984 | 6199666 |
| [unusual cytologic aspects of cervico-vaginal trichomoniasis. study of cervico-vaginal smears stained by the papanicolaou's method]. | trichomonas vaginalis has been recognized by cytologists in papanicolaou-stained smears of cervical and vaginal secretions for some years. the highest percentage of positive trichomonas vaginalis detection was obtained with this technique. classical cytologic aspects of trichomonas vaginalis infection on papanicolaou-stained smears have been commonly reported: cytoplasmic alterations with amphophilia, eosinophilia, perinuclear halo, vacuolisation; nuclear abnormalities with pycnosis, dysplasia-l ... | 1984 | 6203958 |
| inhibition of growth of giardia lamblia by difluoromethylornithine, a specific inhibitor of polyamine biosynthesis. | difluoromethylornithine (dfmo) is a specific and irreversible inhibitor of ornithine decarboxylase, an enzyme which catalyzes the first step in the biosynthetic pathway of the polyamines. we tested the effect of dfmo on the growth of giardia lamblia, entamoeba histolytica, and trichomonas vaginalis. growth of g. lamblia was inhibited by dfmo at concentrations of greater than or equal to 1.25 mm. culture doubling time increased with increasing dfmo concentration. growth inhibition was reversed if ... | 1984 | 6330350 |
| susceptibility of trichomonas vaginalis to metronidazole. | 1984 | 6332727 | |
| [study of antibody responses in rabbits immunized with trichomonas vaginalis using serological reactions]. | 1984 | 6333267 | |
| pyrimidine metabolism in trichomonas vaginalis. | pyrimidine metabolism in trichomonas vaginalis was investigated using washed cell suspensions of the organism with radiolabelled pyrimidine ring precursors and preformed pyrimidines. the precursors [14c]orotate, [14c]bicarbonate and [14c]aspartate were not incorporated into the pyrimidine bases of trichomonal nucleic acids, indicating that the protozoan is unable to synthesise the pyrimidine ring and is dependent on the salvage of exogenous pyrimidines. [3h]uracil, [3h]uridine, [3h]cytidine, deo ... | 1984 | 6333357 |
| trichomonas vaginalis is dependent on uptake and degradation of human low density lipoproteins. | human plasma low density lipoprotein uptake by the urogenital pathogen, trichomonas vaginalis, was examined. rapid binding and internalization of 125i-labeled low density lipoproteins by live t. vaginalis was observed at 37 degrees c. data showing parasite degradation of the internalized apoproteins and lipid accumulation following low density lipoprotein uptake was obtained. acquisition of low density lipoproteins was by a trichomonad surface protein that possessed a molecular weight of greater ... | 1984 | 6333482 |
| a metronidazole-resistant strain of trichomonas vaginalis and its sensitivity to go 10213. | in the present study the comparative in-vitro and in-vivo efficacy of go 10213 was compared with those of metronidazole, secnidazole, tinidazole, ornidazole and nimorazole against a metronidazole-resistant strain of t. vaginalis. go 10213 was found to be superior in activity. it appears more promising than the drugs mentioned above on the basis of the evidence presented. | 1984 | 6334072 |
| acridine orange staining in the identification of trichomonas vaginalis. | 1984 | 6334422 | |
| soluble trichomonas vaginalis antigens in cell-free culture supernatants. | trichomonad proteins present in cell-free supernatants from logarithmic phase cultures of a pathogenic strain of trichomonas vaginalis were detected with enzyme-linked immunosorbent assay employing antibody from mice and rabbit antisera. numerous immunogenic membrane proteins of t. vaginalis were identified by immunoblotting of culture filtrate with rabbit antisera. released membrane antigens ranging in molecular weight from 30 000 to 300 000 under reducing conditions were also detected in radio ... | 1984 | 6334804 |
| [trichomoniasis in pregnancy. cultural and colpocytologic research on 208 asymptomatic pregnant women]. | 1984 | 6334820 | |
| [role of chlamydia, mycoplasmataceae and trichomonas vaginalis in non-gonococcal urethritis in men]. | 1984 | 6334872 | |
| [studies on the animal model of trichomonas vaginalis]. | 1984 | 6335065 | |
| [usefulness of staining specimens with acridine orange in the diagnosis of trichomonas vaginitis]. | 1984 | 6335800 | |
| [antitrichomonal properties of an antiseptic drug cidizol-kb]. | 1984 | 6335801 | |
| new evidence elucidating the mechanism of action of gynatren/solcotrichovac. | 1984 | 6336149 | |
| the destructive effect of solcotrichovac-induced serum antibodies on trichomonas vaginalis; an electron microscopic investigation. | 1984 | 6336150 | |
| [diagnosis, clinical aspects and therapy of vulvovaginitis in daily medical practice]. | 1984 | 6371989 | |
| on the causation of pelvic inflammatory disease. | the classic descriptive model of pelvic inflammatory disease (pid) omits any direct statement that sexual intercourse leads to the development of the lower genital or cervical infection which ultimately leads to upper genital or tubal infection. despite this, clinicians treating patients with pid frequently, either implicity or explicitly, link its onset to sexual activity. in the past decade numerous authors have commented on the strong association between the widespread changes in sexual at ... | 1984 | 6372489 |
| isolation of gardnerella vaginalis from routine genito-urinary tract specimens. | routine screening of 2374 high vaginal swabs yielded 132 cultures (5.6 per cent) which were positive for gardnerella vaginalis. thirty-four of the strains were isolated together with candida albicans and 46 with trichomonas vaginalis. one hundred and twenty (90.9 per cent) of the isolates were from females between the ages of 16 and 40 years. one hundred and nineteen (90 per cent) patients sought medical advice because of a history of discharge (88) or inflammation (31). the other 13 (10 per cen ... | 1984 | 6373950 |
| pathogenicity of trichomonas vaginalis: cytopathologic and histopathologic changes of the cervical epithelium. | virulence of 52 trichomonas vaginalis isolates was estimated by the subcutaneous mouse assay. a positive linear relationship was found between the mean volumes of subcutaneous abscesses caused by the parasites in mice and severity of cervical epithelial abnormalities observed in the patients from whom these strains had been isolated. this relationship implies that virulence of the human urogenital trichomonad, as measured by the mouse assay, may be related to some factors associated with dysplas ... | 1984 | 6377149 |
| selective acquisition of plasma proteins by trichomonas vaginalis and human lipoproteins as a growth requirement for this species. | trichomonas vaginalis avidly bound numerous host macromolecules which were not removed by repeated washing in phosphate buffered saline. the use of radioiodinated cohn plasma fractions in binding studies allowed the identification of plasminogen, fibrinogen, immunoglobulin g, lipoproteins a and b, transferrin, alpha 1-antitrypsin, and albumin on intact organisms. the binding of immunoglobulin g, albumin, transferrin, and lipoproteins to intact, motile trichomonads was further demonstrated using ... | 1984 | 6379453 |
| prostatitis syndromes: pathophysiology, differential diagnosis, and treatment. | prostatitis syndromes are best understood in terms of the anatomy and physiology of the genitourinary tract. infections of the prostate may occur despite numerous host defenses of the male lower urogenital tract. histologic criteria for prostatitis correlate imperfectly with clinical and microbiologic findings. it is critical to distinguish patients with lower-urinary-tract complaints associated with bacteriuria from the larger number of patients without bacteriuria. careful lower-urinary-tract ... | 1984 | 6379919 |
| trichomonas vaginalis, tritrichomonas foetus, and trichomitus batrachorum: comparative proteolytic activity. | at least four proteolytic activities were detected in the lysates of each of trichomonas vaginalis, tritrichomonas foetus, and trichomitus batrachorum. these were hpaase, a dithiothreitol-dependent activity on hide powder azure; azcase, a dithiothreitol-dependent activity on azocasein; and two distinct activities towards peptide nitroanilide derivatives--one was optimally active at ph 7 and stimulated by dithiothreitol; the other had no dithiothreitol requirement and was highly active at ph 5. h ... | 1984 | 6389167 |
| [gardnerella vaginalis infection--another sexually transmitted disease]. | the gardnerella vaginalis infection of the urogenital tract, an std, is of clinical importance in females and of epidemiological importance in males. females suffer from vulvovaginitis amine colpitis, with a bad-smelling grey vaginal discharge with a ph of 5.0-5.5, which contains "clue cells". the urethra of males is often asymptomatically infected. the identification of g. vaginalis is time-consuming and requires a lot of material. isolation and identification of g. vaginalis can not yet be mad ... | 1984 | 6389437 |
| risk factors for prematurity and premature rupture of membranes: a prospective study of the vaginal flora in pregnancy. | prematurity remains a major cause of perinatal mortality in the united states. some research has indicated that infectious agents play a role in either initiating preterm labor, causing premature rupture of the membranes, or preventing tocolysis. this study attempted to determine if the presence of various vaginal pathogens in early pregnancy was associated with the subsequent development of premature rupture of membranes or preterm labor. we found that among 233 evaluable patients those with tr ... | 1984 | 6391179 |
| [value of indirect immunofluorescence and passive hemagglutination reactions in the detection of urogenital trichomoniasis]. | the indirect fluorescent antibody (ifat) and indirect hemagglutination tests (iha) were performed in 375 patients attending the venereal disease clinic (chu of rouen). in addition, wet mount, acridine orange stain and culture were used to detect trichomonas vaginalis in genital discharge. significant levels of antitrichomonas antibodies were present in sera, in 44 per cent of women with vaginal discharge and 23 p. cent of men with urethritis or prostatitis, whereas no evidence of trichomonal inf ... | 1984 | 6391297 |
| double-blind comparative study of trichomonas vaginalis infection: solcotrichovac versus placebo. | 1984 | 6399488 | |
| relationship between contraceptive method and vaginal flora. | this study was undertaken to assess whether the vaginal flora was affected by the method of contraception, and in particular as to whether the incidence of vaginal candidiasis increased when oral contraceptives were used. one thousand and two consecutive vaginal or cervical swabs from women attending a family planning centre were cultured. candida albicans was isolated from 13% of women using no contraception, 16% using oral contraceptives, and from 9%, 19% and 18% of those using diaphragms, int ... | 1984 | 6596089 |