Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| treatment of local complications of bcg vaccination. | 1985 | 4049527 | |
| antiarrhythmic and electrophysiologic actions of clofilium in experimental canine models. | clofilium was studied in three experimental models. in non-ischemic and chronically infarcted canine hearts, clofilium (0.5-2 mg/kg) produced a dose-dependent increase in electrical ventricular fibrillation threshold (vft), but prolonged the effective refractory period (erp) of normal myocardium in only the non-ischemic heart. when chronically infarcted hearts were subjected to programmed electrical stimulation, 1 mg/kg of clofilium inhibited the re-induction of either ventricular tachycardia or ... | 1985 | 4054216 |
| characterization of a mitochondrial transport system for branched chain alpha-keto acids. | efflux of branched chain alpha-keto acids from preloaded rat heart mitochondria was slow at low external ph. efflux was first order, and measured rate constants, kappa efflux, were 0.104 +/- 0.005 and 0.115 +/- 0.006 min-1 for alpha-ketoisovalerate and alpha-ketoisocaproate (kic), respectively. efflux was stimulated significantly by branched chain alpha-keto acids and related carboxylates such as alpha-ketocaproate and alpha-ketovalerate, but not by substrates for the pyruvate transporter. kic w ... | 1985 | 4055776 |
| comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae. | sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ... | 1985 | 4056003 |
| [scintillation assay for evaluating chemosensitivities of human tumors--the basic principle and clinical utility]. | 1985 | 4056552 | |
| cloning of the cytotoxin-hemolysin gene of vibrio vulnificus. | genes encoding the cytotoxin-hemolysin of vibrio vulnificus were cloned in escherichia coli by using the lytic cloning vector, lambda 1059. subcloning in plasmid pbr325 resulted in the isolation of a 3.2-kilobase dna fragment containing the cytotoxin gene. by using this fragment as a dna probe, homologous gene sequences were detected in all 54 v. vulnificus strains studied; homologous sequences were present in none of 96 isolates from 29 other bacterial species. | 1985 | 4066036 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 50-1985. a 41-year-old man with diffuse tracheal narrowing. | 1985 | 4069162 | |
| some properties of vibrio vulnificus hemolysin. | some properties of hemolysin produced by vibrio vulnificus were investigated. the hemolysin was heat labile, and the hemolytic activity was inhibited by adding cholesterol or divalent cations. cholesterol inhibited the temperature-independent hemolysin-binding step, suggesting that cholesterol made up the binding site of the cell membrane, whereas the divalent cations inhibited the temperature-dependent membrane-degradation step. however, the v. vulnificus hemolysin was stable to oxygen and sulf ... | 1985 | 4088099 |
| uptake and clearance of vibrio vulnificus from gulf coast oysters (crassostrea virginica). | oysters collected in late winter, when they were free of vibrio vulnificus, were exposed in the organism in the laboratory. the oysters effectively concentrated the bacteria from seawater, but when the inoculum was removed, the bacteria were rapidly cleared from the oyster tissues. these results suggest that v. vulnificus may be found in oysters as a result of filtration of the bacteria from seawater rather than active multiplication of the bacteria in the oysters. | 1985 | 4091570 |
| isolation and characterization of neuropeptide y from porcine intestine. | the isolation and primary structure of intestinal neuropeptide y (npy) is described. the peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for npy. the amino acid sequence of this peptide is: tyr-pro-ser-lys-pro-asp-asn-pro-gly-glu-asp-ala-pro-ala-glu-asp-leu-ala- arg-tyr-tyr- ser-ala-leu-arg-his-tyr-ile-asn-leu-ile-thr-arg-gln-arg-tyr-nh2. this the structure of intestinal npy is identical to the npy of brain origin. | 1985 | 3838090 |
| chemoattractants in fibrotic disorders. | fibrosis represents an excessive deposition of connective tissue which impedes the normal functions of an organ or tissue. the mechanisms leading to this increased deposition of connective tissue may be similar to those occurring in normal wound repair. we have previously shown that the repair process involves the migration of connective tissue cells to the site of injury and their subsequent proliferation. one of the principal factors controlling these events appears to be the platelet-derived ... | 1985 | 3851719 |
| human c-myc and n-ras expression during induction of hl-60 cellular differentiation. | the genome of the human hl-60 promyelocytic leukemia cell contains amplified c-myc sequences and the transforming n-ras oncogene. the present study has monitored c-myc and n-ras expression in hl-60 cells during induction of myeloid and monocytic differentiation with dimethyl sulfoxide, hexamethylene bisacetamide, 12-o-tetradecanoylphorbol-13-acetate and 1,25 dihydroxy-vitamin d3. the results demonstrate that induction of hl-60 differentiation is associated with decreases in c-myc rna, while ther ... | 1985 | 3856429 |
| selective uptake of a toxic lipophilic anthracycline derivative by the low-density lipoprotein receptor pathway in cultured fibroblasts. | n-(n-retinoyl)-l-leucyldoxorubicin 14-linoleate (r11-dox), a new lipophilic derivative of doxorubicin, was synthesized and incorporated into low-density lipoprotein (ldl). the drug-ldl complex contained 100-200 drug molecules/ldl particle. when cultured normal human fibroblasts were incubated with 125i-ldl-incorporated drug, there was a perfect correlation between the cellular uptake plus degradation of 125i-ldl and the cellular drug accumulation. the presence of excess native ldl inhibited the ... | 1985 | 3856679 |
| in vitro activity of ci-934, a quinolone carboxylic acid active against gram-positive and -negative bacteria. | ci-934 is a totally synthetic difluorinated quinolinecarboxylic acid with an ethyl-amino-methyl pyrrolidine side chain, which has broad-spectrum antibacterial activity, including particular potency directed against streptococci and staphylococci. the ci-934 mic (micrograms per milliliter) for 90% of the strains tested was 0.4 (range, 0.2 to 0.8) for a group of streptococci (pneumococci, viridans streptococci, streptococcus faecalis, and lancefield groups a, b, and c), 0.2 (0.05 to 0.2) for staph ... | 1985 | 3866513 |
| characterization with monoclonal antibodies of a surface antigen of plasmodium falciparum merozoites. | the merozoite, the extracellular form of the erythrocyte stage of the malarial parasite, invades the erythrocyte and develops intracellularly. cloned hybridoma cell lines secreting monoclonal antibodies directed against the merozoite surface were selected by indirect immunofluorescent assay by using intact isolated merozoites. monoclonal antibodies to a 200,000 m.w. merozoite surface antigen were selected and were used to characterize this protein and its role in erythrocyte invasion. immunoelec ... | 1985 | 3881529 |
| cloning and expression in escherichia coli of vibrio parahaemolyticus thermostable direct hemolysin and thermolabile hemolysin genes. | two hemolysin genes of vibrio parahaemolyticus wp1, a thermostable direct (tsd) hemolysin gene and a thermolabile hemolysin gene, were cloned into the pbr322 vector in escherichia coli k-12 c600. a large amount of the tsd hemolysin produced in e. coli k-12 accumulated in the periplasmic space. the tsd hemolysin gene was localized on a 0.9-kilobase hindiii-bamhi fragment by identifying qualitatively the production of the tsd hemolysin by a reverse passive hemagglutination assay in the osmotic sho ... | 1985 | 3886626 |
| cardioprotective effects of defibrotide in acute myocardial ischemia in the cat. | we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ... | 1985 | 3890260 |
| cardioprotective effects of defibrotide in acute myocardial ischemia in the cat. | we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ... | 1985 | 3890260 |
| spontaneous vibrio vulnificus peritonitis and primary sepsis in two patients with alcoholic cirrhosis. | two patients with alcoholic cirrhosis were seen on two separate occasions for fever, swollen legs, petechial hemorrhage, purpura, and cutaneous bullae. one patient ate oysters 2 days before the onset of illness. vibrio vulnificus, a lactose-positive halophilic vibrio, was isolated from the ascitic and cutaneous fluid in both cases, and from the blood in one of the two cases. both isolated strains were sensitive to the antibiotics given to the patients from the beginning; however, both patients d ... | 1985 | 3898820 |
| production and partial characterization of an elastolytic protease of vibrio vulnificus. | conditions are described for the production of large amounts of an extracellular elastolytic protease by vibrio vulnificus. the yield of enzyme was maximal during the late exponential growth phase and was stable during the stationary growth phase in a medium composed of 2% proteose peptone and 1.5% nacl. the protease has a molecular weight of ca. 50,500 (estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis), an isoelectric point of ca. 5.8, and a ph optimum range against azocas ... | 1985 | 3902648 |
| identification of vibrio vulnificus by indirect immunofluorescence. | a rapid, sensitive, and specific indirect immunofluorescence (iif) procedure is described for identifying vibrio vulnificus. reference antisera were prepared by vaccinating rabbits with surface antigen preparations of v. vulnificus, and the antisera were examined for the ability to react with and serologically group 85 isolates of v. vulnificus grown in heart infusion broth, and to detect v. vulnificus in tissue specimens from mice experimentally infected with a virulent isolate of the bacterium ... | 1985 | 3905226 |
| location of polar substituents and fatty acyl chains on lipid a precursors from a 3-deoxy-d-manno-octulosonic acid-deficient mutant of salmonella typhimurium. studies by 1h, 13c, and 31p nuclear magnetic resonance. | eight anionic disaccharide precursors of lipid a accumulate at 42 degrees c in 3-deoxy-d-manno-octulosonic acid-deficient temperature-sensitive mutants of salmonella typhimurium. these compounds comprise a series of lipids based on the minimal structure, o-[2-amino-2-deoxy-n2,o3-bis(3-hydroxytetradecanoyl)-beta-d-glucopyranos yl] -(1----6)-2-amino-2-deoxy-n2, o3-bis(3-hydroxytetradecanoyl)-alpha-d-glucopyranose 1,4'- bisphosphate (designated lipid iva) that differ from each other by the presence ... | 1985 | 3905805 |
| location of polar substituents and fatty acyl chains on lipid a precursors from a 3-deoxy-d-manno-octulosonic acid-deficient mutant of salmonella typhimurium. studies by 1h, 13c, and 31p nuclear magnetic resonance. | eight anionic disaccharide precursors of lipid a accumulate at 42 degrees c in 3-deoxy-d-manno-octulosonic acid-deficient temperature-sensitive mutants of salmonella typhimurium. these compounds comprise a series of lipids based on the minimal structure, o-[2-amino-2-deoxy-n2,o3-bis(3-hydroxytetradecanoyl)-beta-d-glucopyranos yl] -(1----6)-2-amino-2-deoxy-n2, o3-bis(3-hydroxytetradecanoyl)-alpha-d-glucopyranose 1,4'- bisphosphate (designated lipid iva) that differ from each other by the presence ... | 1985 | 3905805 |
| mechanism of sbcb-suppression of the recbc-deficiency in postreplication repair in uv-irradiated escherichia coli k-12. | the mechanism by which an sbcb mutation suppresses the deficiency in postreplication repair shown by recb recc mutants of escherichia coli was studied. the presence of an sbcb mutation in uvra recb recc cells increased their resistance to uv radiation. this enhanced resistance was not due to a suppression of the minor deficiency in the repair of dna daughter-strand gaps or to an inhibition of the production of dna double-strand breaks in uv-irradiated uvra recb recc cells; rather, the presence o ... | 1985 | 3911022 |
| [effect of r plasmids on the growth characteristics of shigella sonnei and escherichia coli]. | revealing of growth characteristics in plasmid and plasmid-free strains was studied with the use of two different hosts: e. coli 15-3 and sh. sonnei 11-941 containing conjugative r plasmids differing in the set of the resistance markers. it was shown that the r plasmids had no noticeable effect on the period of the lag phase and the time of the microbial cell generation. it was also shown that the number of the viable cells in separate cultures of the plasmid-free strain of sh. sonnei 11-941 and ... | 1985 | 3911872 |
| rapid microimmunodiffusion method with species-specific antiserum raised to purified antigen for identification of vibrio vulnificus. | an antigen common to vibrio vulnificus strains, designated vva, was purified by ammonium sulfate precipitation, gel filtration, ion-exchange column chromatography, and preparative gel electrophoresis. the molecular weight of vva was 64,000 when estimated by gel filtration and 40,000 when measured by denaturing polyacrylamide gel electrophoresis. antiserum prepared against purified vva (anti-vva serum) did not agglutinate whole cells of v. vulnificus. therefore, vva was considered a possible inte ... | 1985 | 3918072 |
| demonstration of a common antigen in sonicated cells for identification of vibrio vulnificus serotypes. | ouchterlony immunodiffusion of sonicated vibrio vulnificus cells illustrated a single major precipitation line with antiserum prepared from whole cells of the same species. antigenic analysis by two-dimensional immunoelectrophoresis verified the presence of a single dominant precipitation line. tandem two-dimensional immunoelectrophoretic analyses of v. vulnificus antigens from various strains revealed one fused precipitation line of identity. no fused precipitation lines were seen with other vi ... | 1985 | 3918076 |
| the prostaglandin h synthase-dependent activation of 2-aminofluorene to products mutagenic to s. typhimurium strains ta98 and ta98nr. | 1985 | 3925332 | |
| pressure- and temperature-dependent adhesion of pseudomonas aeruginosa to hep-2 cells. | pseudomonas aeruginosa bacteria adhere to human epitheloid (hep-2) cells in culture. under normal atmospheric conditions (1 ata), this adhesion increased significantly when the temperature rose from 22 to 37 degrees c. under hyperbaric atmosphere (= air, 7 ata) conditions, a similar, significant enhancement of bacterial adhesion to the cells was noted when the temperature rose. if the temperature was kept stable at 22 or 37 degrees c and the pressure was increased from 1 to 7 ata, a pressure-ind ... | 1985 | 3925713 |
| synthesis of dihomoprostaglandins from adrenic acid (7,10,13,16-docosatetraenoic acid) by human endothelial cells. | human umbilical vein endothelial cells were found to contain adrenic acid (22:4) in their cellular lipids. since this fatty acid may be metabolized by cyclooxygenase in the kidney, the metabolism of adrenic acid was studied in endothelial cell cultures. [14c]adrenic acid was metabolized to several more polar metabolites. two of these metabolites co-migrated on hplc with 1 alpha,1 beta-dihomo-8-ketoprostaglandin f1 alpha (the metabolite of 1 alpha, 1 beta-dihomoprostaglandin i2) and 1 alpha,1 bet ... | 1985 | 3931686 |
| de novo expression of t cell markers on theileria parva-transformed lymphoblasts in cattle. | we describe monoclonal antibodies that detect two new membrane antigens on bovine t cells. one molecule is only expressed on activated t cells and has a m.w. of 100,000. the other antigen is a glycoprotein that is precipitated as two bands of m.w. 150,000 and 158,000 and is expressed on a subpopulation of t cells and all myeloid cells. we show that when bovine lymphocytes are transformed by the protozoan parasite theileria parva, both antigens become expressed on the cell surface. the infected c ... | 1985 | 3934278 |
| [an experimental study on adhesion blocking in simultaneous injury of tendon and nerve]. | the formation of adhesions between simultaneously injured tendon and nerve with and without adhesion blocking materials was studied by using a rabbit model. a collagen membrane, produced from adult bovine corium and with a negative electric charge induced by chemical modification, was used as the blocking material. microangiography and histological studies were performed to investigate the vascular supply to the adhesions. the following results were obtained. blood vessel proliferation was obser ... | 1985 | 3831164 |
| [effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. | 1985 | 3835674 | |
| the ada conference on increasing access to dental care. a report. | 1985 | 268525 | |
| drugs and the respiratory system. | 1985 | 261253 | |
| [intravenous administration of amikacin sulfate for the infections complicated with hematologic disorders (author's transl)]. | 1985 | 271749 | |
| [intermittent parasystole, parasystolic slow ventricular tachycardia and ventricular parasystoles: clinical observations and electrophysiological mechanisms]. | 1985 | 261915 | |
| will test tube babies spawn legal problems? | 1985 | 263190 | |
| [pretreatment in periodontal therapy]. | initial treatment is of central importance in dental interventions for marginal periodontal lesions. the hygienic phase must be successfully concluded before further measures of periodontal surgery or bigger reconstructions of any kind are undertaken. in this way optimal prognosis is achieved for all phases of therapy. | 1985 | 271583 |
| parathyroid hormone secretion in magnesium deficiency. | the effect of an acute elevation of the serum magnesium concentration on the concentrations of serum immunoreactive parathyroid hormone (ipth) were studied in hypocalcemic hypomagnesemic patients, hyperparathyroid patients, and normal individuals. basal serum ipth concentrations in the hypomagnesemic patients ranged from undetectable to 3 times the upper limit of normal. all hypomagnesemic patients were observed to have an immediate rise in the serum ipth concentration after magnesium administra ... | 1984 | 263326 |
| iatrogenic foreign body. | 1984 | 271282 | |
| the ldh virus: an interfering biological contaminant. | 1984 | 263259 | |
| [inhibition of premature uterine contractions]. | various drugs used to stop premature uterine contractions are discussed in the paper. particular attention is paid to beta-mimetic drugs. the results of ming partusisten, one of beta-mimetic drugs, is presented on the material of 104 patients with threatening immature and premature labour. partusisten was administered in the form of intravenous drip infusion or tablets. during treatment monitoring of the uterine contractility and of foetal heart rate took place. inhibition of the uterine contrac ... | 1984 | 263521 |
| jaw lymphoma in middle east children. | six cases of jaw lymphoma occurring in middle east children between 1971-1976 are reported. while the histological and radiological appearances of the tumours were suggestive of a diagnosis of burkitt's tumour, the middle east tumour failed to respond clinically to treatment which is effective against burkitt's lymphoma. it is believed that these tumours may constitute a separate clinical entity. the identification of the initiating agent should be a matter of urgency, for treatment at present i ... | 1984 | 271017 |
| [results of the treatment of nonlymphoblastic acute leukemia in a pediatric population]. | on a prospective fashion during approximately two years, 22 pediatric patients with acute non lymphocytic leukemia were evaluated. of this population the majority had acute mielocytic leukemia, followed by acute myelomonocytic leukemia. absolutely all patients at the time of diagnosis and subsequently every 4 to 6 weeks had a bone marrow aspiration test. when the patients were first seen, 54% of them presented fever; lymph node enlargement was not a common finding. only few of this patients pres ... | 1984 | 270999 |
| kinetic investigation of unfolding and partial refolding of a crab satellite (da-dt)n. | crab (da-dt)n was isolated from the testes of cancer borealis by a procedure involving separation of dna and segregation of the satellite fraction by hg2+ binding/cs2so4 density gradient ultracentrifugation. the titration of crab (da-dt)n samples at 10 degrees indicated a sharp absorbance change at ph 11.98 in agreement with the phm value observed for synthetic poly(da-dt) under identical conditions. the reversal of the titration, however, resulted only in about 50% recovery of the original abso ... | 1984 | 240385 |
| protection of mice against vibrio vulnificus disease by vaccination with surface antigen preparations and anti-surface antigen antisera. | vaccination of mice with either formalin-killed cells or cell extracts of a virulent strain and a weakly virulent strain of vibrio vulnificus or with rabbit antisera (as) against the formalin-killed cells and cell extracts protected against the virulent strain of v. vulnificus. however, the virulent strain vaccines and as elicited a significantly stronger immune response than the weakly virulent strain vaccines and as. adsorption of the as with either the homologous or heterologous formalin-kill ... | 1984 | 6469346 |
| halophilic vibrio species from seafish in senegal. | sucrose-positive and sucrose-negative halophilic vibrio species at counts of up to 10(7)/100 g were isolated from muscles tissue in 27 and 43%, respectively, of 128 seafish from coastal waters in senegal. vibrio parahaemolyticus, including 21% urease-positive strains, was the most common isolate, followed by vibrio alginolyticus, vibrio vulnificus, vibrio damsela, and vibrio fluvialis. | 1984 | 6476829 |
| nucleotide base sequence of vibrionaceae 5 s rrna. | nucleotide base sequences of 5 s rrnas isolated from vibrio vulnificus, vibrio anguillarum, and aeromonas hydrophila were determined. comparisons among these and sequences of 5 s rrnas from other species of vibrionaceae provide information useful in the evaluation of the evolution of bacterial species. | 1984 | 6479333 |
| resistance of vibrio vulnificus to serum bactericidal and opsonizing factors: relation to virulence in suckling mice and humans. | vibrio vulnificus causes soft-tissue infections, gastrointestinal disease, and severe sepsis in humans. bacterial and host factors in virulence have remained poorly defined. we found that blood culture isolates of v. vulnificus were completely resistant to the bactericidal effects of 10% normal human serum, in contrast to soft-tissue and environmental isolates that showed a mean 2.6 log10 decline during 120 min of incubation. following opsonization by 10% normal human serum, mean uptake of blood ... | 1984 | 6481186 |
| vibrio vulnificus endometritis. | vibrio vulnificus most frequently causes wound infections contracted after exposure to seawater or primary septicemias resulting from the consumption of raw oysters. we report a case of endometritis caused by v. vulnificus. the infection was apparently acquired during the act of sexual intercourse in seawater in an area in which v. vulnificus has been frequently isolated. the efficacy of treatment with an antimicrobial regimen which included tetracycline is discussed. | 1984 | 6490813 |
| necrotising fasciitis caused by vibrio vulnificus. | a case of necrotising fasciitis caused by vibrio vulnificus is described. the need for early recognition and aggressive surgical treatment are highlighted, and the necrotising infections due to v vulnificus described in the published work are reviewed. | 1984 | 6501592 |
| evidence for the presence of a capsule in vibrio vulnificus. | vibrio vulnificus strain fcc, isolated from a patient with a wound infection, and reference strain atcc 27562, were examined by electron microscopy for the presence of capsules. both strains had a layer heavily stained with ruthenium red. the number of stained cells was high in strain fcc and low in strain atcc 27562. the proportion of stained cells correlated with virulence against mice and with susceptibility to the bactericidal activity of normal human serum. rapid freezing and substitution f ... | 1984 | 6512503 |
| on the serology of vibrio vulnificus. | the serology of 70 strains of vibrio vulnificus was studied. seven o-antigenic groups were defined among them and it was shown that the h antigens of all the strains were identical. a heat-labile masked antigen, which inhibits o agglutination, was found in some strains. as all o antisera for v. vulnificus contained some r antibody, all diagnostic o sera must be absorbed with r organisms before use. | 1984 | 6536784 |
| comparative studies and laboratory diagnosis of vibrio vulnificus, an invasive vibrio sp. | vibrio vulnificus was isolated from a bacteremic patient. this strain, together with other isolates of v. vulnificus, was compared with v. alginolyticus, v. fluvialis, and v. parahaemolyticus with regard to growth characteristics on enteric agar media (enabling isolation and identification) and production of exoenzymes which could correlate with invasive potential. v. vulnificus grew well on macconkey. endo, xylose-lysine deoxycholate, and hektoen enteric agar plates. because v. vulnificus colon ... | 1984 | 6699140 |
| wound infections caused by vibrio vulnificus, a marine vibrio, in inland areas of the united states. | vibrio vulnificus is a halophilic marine vibrio which may produce infection in wounds exposed to seawater or raw shellfish. the centers for disease control has received two isolates from wounds exposed to inland waters, a new mexico creek and an oklahoma reservoir. halophilic organisms were recovered from both the creek and the reservoir, and the water in both sites was found to be brackish. both clinical isolates of v. vulnificus grew in salt concentrations as low as those found in the creek an ... | 1984 | 6699148 |
| cytolytic activity and virulence of vibrio damsela. | a correlation was observed between the ability of 19 isolates of vibrio damsela, a halophilic bacterium recently recognized as a human pathogen, to cause disease in mice and to produce large amounts of a cytolytic toxin in vitro. the yield of toxin in the culture supernatant fluids was optimal during the mid- and late-logarithmic phases of growth in medium containing 0.5% na+ ion, was stable during the stationary growth phase, and was significantly reduced in culture medium containing greater th ... | 1984 | 6715036 |
| clinical features and an epidemiological study of vibrio vulnificus infections. | vibrio vulnificus, a recently described halophilic vibrio species, has been isolated from the blood, wounds, and other skin lesions of patients with primary sepsis or wound infections. because no study of risk factors for infections with v vulnificus has been reported, a case-control study was performed with the 30 patients from whom v vulnificus isolates were recently submitted to the centers for disease control (atlanta, georgia). patients with primary sepsis were more likely than controls to ... | 1984 | 6725989 |
| role of heme compounds and haptoglobin in vibrio vulnificus pathogenicity. | an induced peritonitis model was employed in mice to determine whether heme-containing molecules enhance the lethality of infections by vibrio vulnificus. the lethality of intraperitoneal (ip) inocula of the bacteria was increased by concurrent injections (ip) of hemoglobin, methemoglobin, or hematin, but not by myoglobin. similar results were obtained in mice with phenylhydrazine-induced hemoglobinemia, in which after ip injections of v. vulnificus, a direct correlation between lethality and le ... | 1984 | 6746093 |
| extracellular phospholipase a2 and lysophospholipase produced by vibrio vulnificus. | phospholipase a2 and lysophospholipase activities were detected in the culture supernatant fluids of a virulent strain of vibrio vulnificus. the phospholipase a2 was inactivated by heating at 56 degrees c for 30 min, had an apparent molecular weight of greater than or equal to 80,000 (estimated by gel filtration with sephadex g-75), and a pi of ca. 5.0. phospholipid hydrolysis was unaffected by ca2+ or ethylene glycol-bis(beta-aminoethyl ether)-n,n-tetraacetic acid and was optimal at ph 5.0 to 5 ... | 1984 | 6746100 |
| vibrio vulnificus septicaemia. | a patient, who had been receiving treatment for refractory anaemia with excess of myeloblasts for three years, developed septic shock due to vibrio vulnificus septicaemia. the infection was believed to have been introduced with the ingestion of raw oysters. this organism is comparatively rare, but it can cause serious infection, and is known to be capable of very rapid tissue invasion. | 1984 | 6749004 |
| [a case report of vibrio vulnificus infection with successful treatment]. | 1984 | 6086784 | |
| vibrio hand infections: a case report and review of the literature. | a case of vibrio vulnificus infection of the hand is presented. the role of vibrio species as primary pathogens in infections associated with exposure to a marine environment is discussed. | 1984 | 6386958 |
| [case of bacteremic phlegmon due to vibrio vulnificus]. | 1983 | 6420485 | |
| vibrio vulnificus septicemia. isolation of organism from stool and demonstration of antibodies by indirect immunofluorescence. | vibrio vulnificus was isolated from blood and stool cultures from a 65-year-old man who had underlying alcoholic liver disease. the patient had eaten raw oysters the day before he became ill. to our knowledge, this is the first published report of isolation of the organism from stool in a patient with primary septicemia, and it provides support for epidemiologic studies suggesting that the infection is acquired through the gastrointestinal tract by eating raw seafood containing the organism. it ... | 1983 | 6340632 |
| siderophore production by vibrio vulnificus. | previous studies in our laboratory, as well as clinical evidence, have suggested that increased iron levels in the host may be important in infections caused by the halophilic pathogen vibrio vulnificus. to study iron acquisition, we induced siderophore production by growth in a low-iron medium, and biochemical testing indicated the production of both hydroxamate- and phenolate-type siderophores. the siderophores were extracted from growth filtrates with ethyl acetate (for phenolates) and phenol ... | 1983 | 6223882 |
| vibrio vulnificus. a gastronomic hazard. | 1983 | 6572281 | |
| spectrum of vibrio infections in a gulf coast community. | infection with vibrio species was diagnosed in 23 patients over a 10-year period in a gulf coast community. species isolated were vibrio vulnificus, v. cholerae, v. parahaemolyticus, and v. alginolyticus. clinical presentations included diarrhea, wound infection, and bacteremia. gastrointestinal tract infection was infrequently diagnosed, despite the routine use of stool culture media appropriate for vibrio isolation. bacteremic infections were severe and occurred only in patients with underlyin ... | 1983 | 6625378 |
| [vibrio vulnificus]. | 1983 | 6635466 | |
| differential complement activation and susceptibility to human serum bactericidal action by vibrio species. | the ability of vibrio vulnificus to resist human serum bactericidal action and to activate human complement was compared with similar cultures of vibrio cholerae and vibrio parahaemolyticus. both v. vulnificus and v. parahaemolyticus had similar survival rates in sera and were much more resistant to killing than was v. cholerae. in contrast, v. vulnificus activated significantly less serum complement than did v. cholerae and v. parahaemolyticus. the relative ability of v. vulnificus to survive i ... | 1983 | 6642665 |
| [fatal vibrio vulnificus infection in a patient with aplastic anemia]. | the patient was a 59-year-old man who had been in hospital suffering from aplastic anemia with transfusion hemosiderosis. sudden onset of weakness, shaking chills and headache was observed after his staying out overnight on july 25, 1981. his temperature was 39.3 degrees c and he complained of abdominal pain and abdominal distension. his blood pressure dropped to a dangerous level and tonic convulsions that had begun in the upper body gradually extended to the whole body and he died 23 hours aft ... | 1983 | 6679624 |
| marine noncholera vibrio infections in florida. | in the past decade, there has been a dramatic increase in the number of reported cases of clinical illness from noncholera (marine) vibrio infections, leading to a greater recognition of this potentially lethal disease. the wide spectrum of pathogenicity of this organism is reflected in the six cases in this report, varying from simple gastroenteritis to focal necrotizing cellulitis to fatal septicemia. we have reviewed the fundamental clinical differences between vibrio vulnificus and other non ... | 1983 | 6828895 |
| extraintestinal infections due to halophilic vibrios. | to compare the clinical and epidemiologic features, we reviewed the hospital records and interviewed 18 patients with extraintestinal infections caused by three species of halophilic vibrios. vibrio vulnificus, the organism most frequently isolated in virginia during the six-year study period, caused primary septicemia in three patients. fifteen patients had soft tissue infections, most of which followed injuries that were contaminated by seawater and mostly caused by v vulnificus and v parahaem ... | 1983 | 6844963 |
| distribution of vibrio vulnificus and other lactose-fermenting vibrios in the marine environment. | during the summer of 1981, 3,887 sucrose-negative vibrios were isolated from seawater, sediment, plankton, and animal samples taken from 80 sites from miami, fla., to portland, maine. of these, 4.2% were able to ferment lactose. the lactose-positive strains isolated from the various samples correlated positively with ph and turbidity of the water, vibrios in the sediment and oysters, and total bacterial counts in oysters. negative correlations were obtained for water salinity. numerical taxonomy ... | 1983 | 6847190 |
| antibiotic efficacy against vibrio vulnificus in the mouse: superiority of tetracycline. | seven antimicrobial agents, all effective against vibrio vulnificus in vitro, were compared for in vivo efficacy in mice experimentally infected with v. vulnificus strain b3547. mice were injected s.c. with 1 x 10(8) cells, and i.p. injection of antimicrobials was begun 1.5 hr later when mice were bacteremic and had edematous lesions at the injection site. the study was done in two phases. phase i was a dose-ranging experiment, using single injections within the range (on a body weight-adjusted ... | 1983 | 6864521 |
| new selective plating medium for isolation of vibrio vulnificus biogroup 1. | a new plating medium (vv agar) has been developed as an alternative to thiosulfate-citrate-bile salts-sucrose (tcbs) agar for the isolation of vibrio vulnificus. salicin (2% wt/vol) is employed as the source of carbohydrate, with potassium tellurite (0.0005% wt/vol), crystal violet (0.00015% wt/vol), oxgall (0.8% wt/vol), and a ph of 8.6 to inhibit growth of gram-positive and gram-negative organisms other than v. vulnificus. because strains of v. vulnificus do not strongly ferment salicin in vv ... | 1983 | 6874897 |
| vibrio vulnificus infection. case report and update since 1970. | vibrio vulnificus infections is being reported with increasing frequency in coastal regions of the united states. raw seafood consumption, particularly raw oysters, and wounds acquired in a marine environment predispose to infection. patients with advanced liver disease are at increased risk of developing septicemia. v. vulnificus is a virulent pathogen producing significant morbidity and mortality; its virulence relates in part to the production of exotoxin. skin lesions occur early in the clin ... | 1983 | 6886115 |
| measures of fitness and demographic stability. | the concepts of entropy and reproductive potential of a genotype were introduced in a previous paper [demetrius, l. (1974) proc. natl. acad. sci. usa 71, 4645-4647] and an analoge of the fundamental theorem of natural selection was derived. this paper relates to entropy of a population with the rate of convergence of the population to the stable age distribution. i show that (i) at maximal entropy, no oscillatory components exist and the birth sequence is unaffected by perturbations in the stabl ... | 1983 | 264691 |
| [fibrous dysplasia]. | fibrous dysplasia of bone may occur also in the facial skeleton. main locations are the lateral midface and the mandible. the foci, which are sharply circumscribed in most cases, produce tumour-simulating deformities of single of several facial bones and, in part, disorders of the articulation are bulbar displacements. in contrast to paget's disease, fibrous dysplasia is a disorder of the younger age groups. apart from bony changes, albright's syndrome (which occurs in women only) comprises also ... | 1983 | 272797 |
| [continued education of teachers--reflections about a model of interrelationships]. | 1983 | 242401 | |
| gastric secretion, gastroesophageal reflux and esophagitis. | neither gastroesophageal reflux nor esophagitis were found to have a clear-cut relationship with gastric secretory output. patients with esophagitis had significantly longer periods of reflux than those without esophagitis. it is concluded that the important factor in the genesis of esophagitis is the duration of contact between the esophageal mucosa with gastric contents rather than gastric secretory output. | 1982 | 242213 |
| [bronchial asthma due to the "trigger mechanism" (foreign bodies in the bronchi; hiatal hernia): presentation of clinical cases]. | 1982 | 242070 | |
| validity of family informants' ratings of psychiatric patients: general validity. | 1982 | 242027 | |
| two dental schools. | 1982 | 351169 | |
| collagenolytic activity of vibrio vulnificus: potential contribution to its invasiveness. | vibrio vulnificus (lactose-positive vibrio) produced collagenase when grown in 2% synthetic sea salts supplemented with hydrolyzed casein. the addition of collagen or peptone to the medium increased the level of collagenase production. collagenase activity was inhibited by edta but not by fetal calf serum. | 1982 | 6279515 |
| [a case of hepatoma associated with vibrio vulnificus bacteremia and its bacteriological findings]. | 1982 | 6290579 | |
| [clinical and bacteriological studies on a case with infectious disease caused by vibrio vulnificus]. | 1982 | 6819326 | |
| statistical evaluation of a quality control method for isolation of pathogenic vibrio species on selected thiosulfate-citrate-bile salts-sucrose agars. | the recovery of vibrio cholerae, vibrio fluvialis, vibrio parahaemolyticus, and vibrio vulnificus, employing eight strains of each species, was studied by using four brands of thiosulfate-citrate-bile salts-sucrose (tcbs) agar prepared according to manufacturers' instructions and following a standardized procedure. a standardized broth inoculum of each strain was placed on duplicate plates of each brand of tcbs agar and also on tryptic soy agar (difco laboratories) containing 1% (wt/vol) nacl, t ... | 1982 | 6761362 |
| diluent composition for use of api 20e in characterizing marine and estuarine bacteria. | nine chemically defined inoculation diluents, with compositions ranging from 0.85% nacl to 35% marine salts, were used to evaluate the influence of diluent composition on the biochemical profiles of 30 marine and estuarine bacterial strains, including species of vibrio, aeromonas, allomonas, and photobacterium. results demonstrated that a 20% marine salts diluent enabled the characterization of halophilic strains normally nonreactive by the api 20e system. furthermore, the use of 20% marine salt ... | 1982 | 7125655 |
| vibrio vulnificus biogroup 2: new biogroup pathogenic for eels. | clinical and nonclinical isolates of the lactose-positive vibrio vulnificus were compared with vibrio strains isolated from lesions on eels (anguilla japonica) cultured commercially in japan. strains were compared phenotypically and antigenically, for pathogenicity to mice and eels, and for genetic relatedness. the strains isolated from diseased eels differed phenotypically from the original species description of v. vulnificus in that they were negative for indole production, ornithine decarbox ... | 1982 | 7138004 |
| disease caused by a marine vibrio--vibrio vulnificus. | 1982 | 7144852 | |
| distribution and ecology of vibrio vulnificus and other lactose-fermenting marine vibrios in coastal waters of the southeastern united states. | water, sediment, plankton, and animal samples from five coastal sites from north carolina to georgia were sampled for their lactose-fermenting vibrio populations. over 20% of all vibrios tested were sucrose negative and o-nitrophenyl-beta-d-galactopyranoside (onpg) positive, suggesting identification as the human pathogen vibrio vulnificus. these vibrios were isolated from all sample sites and sources (water, sediment, plankton, and animals). correlations with several of 19 environmental paramet ... | 1982 | 7159083 |
| isolation and characterization of vibrio vulnificus from two florida estuaries. | vibrio vulnificus was enumerated in seawater and shellfish from two florida estuaries at selected seasonal intervals. there were significant fluctuations in the presence and numbers of v. vulnificus. relatively high seawater temperature and salinity favored the presence of v. vulnificus in both seawater and shellfish samples. | 1982 | 7159088 |
| vibrio vulnificus septicemia in a patient with liver cirrhosis. | 1982 | 7187150 | |
| lethal cold stress of vibrio vulnificus in oysters. | studies were conducted on the survival of vibrio vulnificus, an estuarine human pathogen, in oyster homogenates held at 4 degrees c. results indicated a rapid and dramatic decrease in viability not attributable to either cold shock or the oyster homogenate alone but to a combination of the two. such a decline was not observed with vibrio parahaemolyticus. chilled v. vulnificus cells were unable to repair themselves in brain heart infusion broth at 37 degrees c. v. vulnificus cells incubated on w ... | 1981 | 7224632 |
| acute bacterial myositis caused by vibrio vulnificus. | 1981 | 7241733 | |
| edema and hemoconcentration in mice experimentally infected with vibrio vulnificus. | vibrio vulnificus (lactose-positive vibrio), a recently recognized pathogenic marine species, produced extreme hemoconcentration and death within 3 to 6 h after subcutaneous or intraperitoneal injection of 10(8) viable cells into mice; hemotocrit values approached 70% (normal, 45%). about 1 ml of edema fluid accumulated at the site of each subcutaneous injection, and locally increased vascular permeability was demonstrated by a skin bluing assay, using evans blue dye. a corresponding fluid accum ... | 1981 | 7251164 |
| wound infection and septic shock due to vibrio vulnificus. | vibrio vulnificus is an organism commonly found in the marine environment whose pathogenic potential for humans has been recently recognized. two patients are described who developed rapidly progressing wound infections and bacteremia due to this halophilic, lactose-positive vibrio. the clinical manifestations of the resulting infection differed from patterns reported by other authors. | 1981 | 7258496 |
| interaction of vibrio vulnificus with human polymorphonuclear leukocytes: association of virulence with resistance to phagocytosis. | the results of studies described in this report support the ideas that virulence of vibrio vulnificus is associated, at least in part, with resistance to phagocytosis and that the ability of the bacterium to resist phagocytosis results from its possession of an antiphagocytic surface antigen. these conclusions are based on the observations that (1) human polymorphonuclear leukocytes (pmnls) exhibited a significantly weaker chemiluminescent response and phagocytic response when interacting with a ... | 1981 | 7276636 |
| hemolytic reaction of clinical and environmental strains of vibrio vulnificus. | all vibrio vulnificus strains tested (four clinical isolates and eight environmental isolates) hemolyzed human erythrocytes. in contrast to findings with vibrio parahaemolyticus, in which hemolytic activity correlates with isolation from clinical specimens, results from the present study suggest that hemolysis is not usefull in differentiating v. vulnificus strains. | 1981 | 7287896 |