Publications

[etiology of dairy cows mastitis in slovakia in the years 1972-1974].	in the period from 1972 to 1974, veterinary institutes in slovakia performed bacteriological examinations in 178,853 milk samples coming from 72,454 dairy cows. on an annual average, the number of examined samples were higher by 61% than in 1967-1971. germs responsible for the inflammation of the mammary gland were found in the milk of 22.32% of the dairy cows. the following bacterial germs were represented: streptococcus agalactiae -- 15.77%, staphylococcus aureus -- 4.19%, other streptococci ...	1975	813355
bactericidal and haemolytic activity of complement in bovine colostrum and serum: effect of proteolytic enzymes and ethylene glycol tetraacetic acid (egta).	bovine colostral whey (cw) possessed heat-labile bactericidal activity against an enteropathogenic serum-susceptible strain of escherichia coli. activity was readily destroyed by trypsin or rat pancreatic juice, and less readily by chymotrypsin. loss of activity due to trypsin, but not by chymotrypsin, could be prevented by addition of excess bovine colostral trypsin inhibitor. a second enteropathogenic strain of e. coli was killed by cw only if exogenous complement was added. this activity was ...	1975	813560
[etiology of mastitis in dairy cows in slovakia during 1972-1974].	in the period from 1972 to 1974, veterinary institutes in slovakia performed bacteriological examinations in 178,853 milk samples coming from 72,454 dairy cows. on an annual average, the number of examined samples was higher by 61% than in 1967-1971. germs responsible for the inflammation of the mammary gland were found in the milk of 22.32% of the dairy cows. the following bacterial germs were represented: streptococcus agalactiae - 15.77%, staphylococcus aureus - 4.19%, other streptococci - 1. ...	1975	814671
indirect consequences of low-level use of antimicrobial agents in animal feeds.	antibiotics may be classified as "therapeutic" or "feed" depending on their use for treatment of disease or for promotion of growth in animals. the mechanism of growth promotion is largely unknown, but it has been shown that the specific feed additive causes defined lesions in the cell wall of escherichia coli. this effect may sensitize the bacteria to the action of therapeutic drugs. damage to the bacterial cell wall may also cause the bacteria to be more susceptible to the body defense mechani ...	1975	1090454
inhibition of escherichia coli by bovine colostrum and post-colostral milk. i. complement-mediated bactericidal activity of antibodies to a serum susceptible strain of e. coli of the serotype o 111.	bovine colostral whey diulted in unheated bovine milk or in kolmer saline containing 5 per cent pre-colostral calf serum was bactericidal for escherichia coli nctc 9703 (serotype o 111). whey diluted in saline without pre-colostral calf serum was inactive. bactericidal activity was abolished by heating at 56 degrees for 30 minutes or by pre-incubating with n-acetyl-l-tyrosine ethylester, indicating that complement present in milk or pre-colostral calf serum was involved in the bactericidal activ ...	1975	1090521
calf ligated intestinal segment test to detect enterotoxigenic escherichia coli.	the ligated intestinal segment test in the young calf was utilized to establish the enterotoxigenicity of approximately 600 escherichia coli isolates obtained from fecal specimens intestinal contents of calves with diarrheal disease. one hundred and fifty isolates were routinely tested in a single calf. there was little problem with false-positive reactions. false-negative reactions normally occurred only if the isolate was tested in the posterior 3 m of the small intestine. the ligated small in ...	1975	1090536
[bacteriocinogeny in yersinia enterocolitica].	bacteriocinogeny was found in 19.8 per cent of y. enterocolitica strains tested. it was designated by the authors as enterocoliticacinogeny and the substance produced by the pathogen was named enterocoliticacine. bacteriocinogeny was found among the collection strains isolated in the south primorye and not among the strains isolated abroad. some cultures of y. enterocolitica isolated abroad and in the south primorye were used as indicator strains for revealing enterocoliticacinogeny. enterocolit ...	1975	1091204
deoxyribonucleic acid-cytosine methylation by host- and plasmid-controlled enzymes.	deoxyribonucleic acid (dna)-cytosine methylation specified by the wild-type escherichia coli k 12 mec+ gene and by the n-3 drug resistance (r) factor was studied in vivo and in vitro. phage lambda and fd were propagated in the presence of l-[methyl-3h]methionine in various host bacteria. the in vivo labeled dna was isolated from purified phage and depurinated by formic acid-diphenylamine treatment. the resulting pyrimidine oligonucleotide tracts were separated according to size and base composit ...	1975	1091619
enzymatic arginylation of beta-melanocyte-stimulating hormone and of angiotensin ii.	porcine beta-melanocyte-stimulating hormone and angiotensin ii were examined as acceptors in the reaction catalyzed by arginyl-trna-protein transferase. both inhibited enzymatic transfer of [14c]arginine from trna to bovine albumin. inhibition was competitive with albumin and the k-i values were, respectively, 15 and 0.8 mum. the expected arginylated compounds were isolated and characterized. beta-melanocyte-stimulating hormone and its arginylated product had identical activities in the frog epi ...	1975	1091639
defective mononuclear leukocyte chemotaxis in the chediak-higashi syndrome of humans, mink, and cattle.	chemotaxis of mononuclear leukocytes from humans, mink, and cattle was evaluated in vitro using a morphologic boyden chamber technique and a new 51-cr-labeled mononuclear radioassay with a double micropore filter system. significantly decreased mononuclear leukocyte chemotactic response were noted when human, mink, or cattle chediak-higashi cells were tested using autologous serum or endotoxin-activated autolotous serum. a similar chediak-higashi mononuclear leukocyte defect was noted in humans ...	1975	1092386
enzymatic multiplication of a chemically synthesized dna fragment.	a synthetic dna fragment of 19 residues was enlarged by the enzymatic addition of deoxyadenylate residues to its 3'-end with calf thymus terminal deoxynucleotidyl transferase. the 3'-terminus of this elongated dna strand was blocked with 2', 3'-dideoxyadenylate to prevent hydrolysis by the 3'-exonuclease function of e. coli dna polymerase i. this elongated and 3'-blocked fragment was annealed to an oligomeric primer and used as a template for the synthesis of a complementary copy of the syntheti ...	1975	1093143
quantitation of amanitins in amanita verna with calf thymus rna polymerase b.	a procedure utilizing the specific inhibition of calf thymus dna-directed rna polymerase b has been applied to the quantitation of amanitins. this procedure has permitted the accurate quantitation of alpha-amanitin in amounts as low as 0.05 nanogram, a sensitivity 2000-fold greater than chemical detection methods used following tlc. analysis of extracts of specimens of amanita verna identified by morphological criteria has demonstrated that while toxin concentration is variable, some specimens ...	1975	1094215
some effects of calcium and magnesium ions on the activity of bovine pancreatic deoxyribonuclease a.	bovine pancreatic deoxyribonuclease requires divalent metal cations for hydrolysis of dna. the effects of calcium and magnesium, alone and combined, on the rate and kinetics of the reaction were examined. divalent metal salts of dna were used as substrates. the ratio of either ca-2+ or mg-2+ to dna-p in these salts was 1:2. the mg-2+ salt of dna was found to have sufficient mg-2+ for optimal dnaase activity. addition of mgcl-2 to a large excess of mg-2+ over dna-p had no effect on the rate. km f ...	1975	1095074
[problems of resistance (author's transl)].	the results of sensitivity tests performed during the period from 1970 up to 1973 inclusive are compared with those obtained in pervious years. this showed that resistance to penicillin had increased in staphylococci causing bovine mastitis. during each single year, however, there was a decrease. s. typhimurium isolated from cattle showed increased resistance to chloramphenicol, neomycin and ampicillin; s. dublin showed an increase in resistance to chloramphenicol. e. coli isolated from calves w ...	1975	1096359
the effects of overloading in density-gradient centrifugation.	the effects of overloading of the sample zone in density gradient centrifugation have been studied by use of a three-component shelf-lavered sample in which the total protein concentration was increased by addition of different amounts of albumin. it is found that overloading of the gradient gives rise to particle movements which are not predictable from the svedberg equation. the two typical effects of overloading are dislocation of the zone mass centres and changes in the zone shapes. it is fo ...	1975	1097246
the effects of organic solvents on escherichia coli dna polymerase iii.	the polymerizing ability of escherichia coli dna polymerase iii is enhanced by a variety of water-miscible organic solvents of which dimethyl sulfoxide at 17% (v/v) is the most effective tested. the extent of stimulation depends on the organic solvent used and its concentration, but shows no obvious correlation with the chemical structure of the solvent or its dielectric constant. kinetic studies indicate that the mechanism of stimulation is complex.	1975	1098695
distribution of r plasmids among the o-antigen types of escherichia coli isolated from human and animal sources.	the o-antigen types of 600 independently isolated escherichia coli strains from human feces have been determined, and the types have been related to the antibiotic resistance patterns of the strains. the relative abundance of each o-antigen type differed in the susceptible and resistant series of strains. the majority (86%) of the resistant strains carried r plasmids. resistant e. coli (20.3%) were found associated with o-antigen types 8, 9 and 101, whereas the susceptible strains covered a wide ...	1975	1101815
hematology of the neonatal calf. ii. response associated with acute enteric infections, gram-negative septicemia, and experimental endotoxemia.	the hematological responses of neonatal calves with acute enteric infections were compared to the responses of calves with septicemia and experimentally induced endotoxemia. the mean hematocrit of septicemic calves (45.0% +/- 7.8) was similar to that of calves with primary enteric infections (45.3% +/- 7.0) but the total plasma protein concentration of septicemic calves (5.8g/100 ml +/- 0.69) was significantly lower than that of calves with primary enteric infections (8.6 g/100 ml +/- 1.5). the ...	1975	1104259
[sodium thiocyanate as a protective agent in intensive calf fattening. brief report].	daily administration of sodium thiocyanate at 750 mg a calf for the first three weeks in the fattening stalls reduced the occurrence of illness by 60%. this protective effect seemed to persist during the following weeks without additional treatment. addition of the thiocyanate to the food may be beneficial through a nutritive effect.	1975	1106349
diverse effects of bursectomy on humoral immune responses in the chicken.	chickens bursectomized hormonally or surgically at various ages in embryonic to postembryonic lives were immunized with a mixture of antigens, and the antibodies produced titrated. immune responses to salmonella pullorum (sp), brucella abortus (ba). staphylococcus aureus (staph) and ovalbumin (oa) were more markedly suppressed by the bursal deprivation than those to heterogenous erythrocytes, viruses and such bacteria as pasteurella multocida (pm) and escherichia coli b. the immune response to b ...	1975	1187514
herd incidence of bovine mastitis in four danish dairy districts. i. the prevalence and mastitogenic effect of micro-organisms in the mammary glands of cows.		1974	4213559
infection and immunoglobulin concentrations in chediak-higashi mice.	the chediak-higashi syndrome (chs) has been reported in man, cattle, mink, and mice. chs humans and cattle have an increased incidence of pyogenic infections, whereas chs mink are more susceptible to aleutian disease. age- and sex-matched groups of chs mice (mutant strain) and c57 bl/6n (parent strain) were challenged with candida albicans, escherichia coli, klebsiella pneumoniae, and staphylococcus aureus intravenously and streptococcus pneumoniae intraperitoneally. a significant increase (p < ...	1974	4601767
growth of indigenous organisms in aerated filtrate of feedlot waste.	filtrates from feedlot waste were incubated under aerobic conditions to evaluate the availability of nutrients for cell production and to assess the capacity of indigenous flora to produce stabilized effluents. incubation was carried out in 9-liter aerated jar fermentors. three-fourths of the organic material and one-third of the nitrogen were taken up in 4 days; 90% utilization of organic material and nitrogen required almost a month. acid was produced initially, but aerated liquid thereafter r ...	1974	4609184
continuous non-therapeutic use of antibacterial drugs in feed and drug resistance of the gram-negative enteric florae of food-producing animals.	fecal samples from illinois farm animals and montana range cattle were examined for the frequency of gram-negative enteric organisms resistant to oxytetracycline, dihydrostreptomycin, or ampicillin. the illinois farm animals were continually fed rations containing various antibacterial drugs. the montana range cattle had minimal exposure to antibacterial drugs. the majority of samples from illinois farm animals demonstrated 10% or more antibiotic-resistant organisms. in contrast, resistant organ ...	1974	4615630
isolation of repeated and self-complementary sequences from e. coli dna.	we have used the single-strand specific nuclease from neurospora crassa and chromatography on methylated albumin-kieselguhr to purify and characterize repeated and self-complementary sequences from escherichia coli dna. approximately 0.5% of the genome renatures spontaneously at zero time and another 2% renatures somewhat more rapidly than the total dna. the early renaturing dna has a base composition and a tm similar to the total dna and contains on the average 100 base pairs; the self-compleme ...	1974	10793709
comparative studies on nicotinamide nucleotide transhydrogenase from different sources.	(1) nicotinamide nucleotide transhydrogenases in submitochondrial particles from beef heart mitochondria, chromatophores from rhodospirillum rubrum and membrane preparations from escherichia coli and pseudomonas aeruginosa have been compared with respect to the following properties: stereospecificity for the 4-hydrogen of nadh, reactivity with 3'-nadp, inhibition by palmityl-coa, sensitivity tot rypsin, and effects of ca2+ and 2'-amp on the reaction rates. (2) transhydrogenases from submitochond ...	1974	19400036
a technique for ultracryotomy of cell suspensions and tissues.	ultracryotomy of fixed tissue has been investigated for a number of years but, so far, success has been limited for several reasons. the simple technique herein reported allows the ultracryotomy not only of a variety of tissues but also of single cells in suspension, with a preservation and visualization of ultrastructural detail at least equivalent to that obtained with conventional embedding procedures. in this technique, sucrose is infused into glutaraldehyde-fixed tissue pieces before freezi ...	1973	4121290
modification of rna polymerase after t3 phage infection of escherichia coli b.	e. coli b cells infected with t3 phage contain a modified host rna polymerase in addition to the normal rna polymerase found in uninfected cells. the modified rna polymerase behaves differently in its elution properties from the normal enzyme on deae-cellulose, phosphocellulose, and dna-cellulose column chromatography. the modified enzyme also differs from the normal polymerase in some of its enzymatic parameters. the specific activity of the modified rna polymerase is markedly lower (i.e., (1/4 ...	1973	4126728
studies on the chick-lethal toxin of eecherichia coli.	a toxin which is lethal for two week old chicks has been recovered from strains of escherichia coli o78:k80 of bovine and avian origin and from avian isolates of serogroups o2, o45 and o109. the toxin is heat-labile, antigenic, high in protein, inactivated by pronase, trypsin, amylase, and pancreatic lipase. the toxin may be precipitated by ammonium sulfate or tca treatment from the supernatant obtained by repeated centrifugation of sonicated cells. considerable purification has been obtained by ...	1973	4270809
the kinetics of hydrolysis of synthetic glucuronic esters and glucuronic ethers by bovine liver and escherichia coli beta-glucuronidase.	1. the relative rates of hydrolysis of synthetically prepared beta-d-glucuronic esters [aglycone: benzoic acid, veratroic (3,4-dimethoxybenzoic) acid, indol-3-ylacetic acid and ethylbutyric acid], and beta-d-glucuronic ethers (aglycone: phenolphthalein, p-nitrophenol, 3,4-dimethoxyphenol, 3,4-dimethoxybenzyl alcohol) by commercial preparations of beta-glucuronidase from bovine liver and escherichia coli were investigated. the rates of hydrolysis of all compounds tested were followed by measuring ...	1973	4584027
platelet interaction with bacteria. 3. ultrastructure.	the interaction of several common strains of bacteria with rabbit or human platelets in vitro has been examined sequentially with scanning and transmission electron microscopy. bacteria were added to platelets in their native plasma or to washed platelets in a balanced salt solution at ratios of about 1:1 or at low bacteria to platelet ratios (down to 1:100). the platelet-bacterial interaction (pbi) was studied with recording nephelometry. matched samples were fixed for microscopy at various poi ...	1973	4632008
[r factors in strains of pathogenic enterobacteria isolated from domestic animals and particularly from dogs].	strains of enterobacteria (nine escherichia coli and two salmonella) isolated from primary or secondary infections in the dog, cat, pig, calf and kangaroo were studied for the presence of extrachromosomal drug resistance factors (r factors). seven strains of e. coli and two strains of salmonella transferred resistance involving the following antibiotics: streptomycin, ampicillin, chloramphenicol, neomycin and tetracycline. all strains harboring r factors transferred streptomycin resistance and t ...	1972	4258542
degradation of dna rna hybrids by ribonuclease h and dna polymerases of cellular and viral origin.	ribonuclease h from human kb cells, chick embryos, calf thymus, avian myeloblastosis virus, and rous associated virus specifically degrades the rna of dna.rna hybrids, producing mono- and oligoribonucleotides terminated in 5'-phosphates. the cellular rnase h is an endonuclease, whereas the viral enzyme appears to be an exonuclease. viral dna polymerase and rnase h copurify through all separation steps. therefore, rnase h activity is an intrinsic part of the viral dna polymerase. dna.rna hybrids ...	1972	4343966
phagocytosis of microbial l-forms.	little data are available regarding l-form phagocyte interactions. due to difficulty with more conventional methods, a serum-free technique of surface phagocytosis employing cellulose ester micropore filters, fluorochrome staining, and reflecting fluorescence microscopy was developed. intracellular localization of l-frms was confirmed by electron microscopy. in timed experiments, phagocytosis of a group d streptococcus (atcc 23241) and its l-form (t53i) were maximal at 30 min, although the bacte ...	1972	4628960
anti-gamma globulins and chronic infection: comparative studies of the immune response to various bacteria and gamma globulin preparations.	a study of the relationship of clinical states associated with prolonged infection (bacterial endocarditis and osteomyelitis) and generation of serum anti-gamma globulins was made with particular reference to quantitative amounts of staphylococcal protein a in various infecting strains. no correlation between individual strain amounts of protein a and presence of anti-gamma globulins was detected. thirty-eight rabbits were immunized intravenously with various strains of bacteria (staphylococcus ...	1972	4404684
[practical experiences in the prevention and therapy of most important calf and young cattle diseases in large herds].		1972	4569838
transcription of brain chromatin by ribonucleic acid polymerases from brain nuclei and from escherichia coli.	1. transcription of ox brain chromatin by brain nuclear rna polymerase ii and escherichia coli rna polymerase was studied. 2. the soluble chromatin prepared from brain nuclei contained dna, rna, histone and non-histone proteins. such chromatin preparations did not display any endogenous rna polymerase activity, when assayed in the presence of concentrations of kcl as high as 0.4m. 3. the chromatin-templated activity of brain nuclear polymerase ii was stimulated by kcl, with an optimum around 0.2 ...	1972	4571299
[studies, in the field, of a polyvalent vaccine against pulmonary infections in cattle].		1972	4573942
antigenic relationships in mammalian dna polymerase.	rabbit antibody was prepared against a high-molecular-weight dna polymerase purified from the soluble fraction of calf thymus gland. this antibody does not inhibit terminal deoxynucleotidyl transferase isolated from that source, but does inhibit both low-molecular-weight and high-molecular-weight dna polymerases isolated from cytoplasmic and nuclear fractions of a number of mammalian tissues (mouse l cells, calf thymus, phytohemagglutinin-stimulated human lymphocytes, rat liver, and rabbit bone ...	1972	4110484
invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a neurospora mutant and a component of molybdenum-enzymes.	an active neurospora-like assimilatory nadph-nitrate reductase (ec 1.6.6.2), which can be formed in vitro by incubation of extracts of nitrate-induced neurospora crassa mutant nit-1 with extracts of (a) certain other nonallelic nitrate reductase mutants, (b) uninduced wild type, or (c) xanthine oxidizing and liver aldehyde-oxidase systems was also formed by combination of the nit-1 extract with other acid-treated enzymes known to contain molybdenum. these molybdenum enzymes included (a) nitrogen ...	1971	4399835
the hygiene and marketing of fresh cream as assessed by the methylene blue test. a report by a working party to the director of the public health laboratory service.	the hygiene and marketing of fresh cream in england and wales was investigated by a working party of the public health laboratory service (phls) between 1 october 1968 and 31 july 1969. thirty-one cream-producing dairies were visited and observations made in the light of the code of practice published by the milk and milk products technical advisory committee of the ministry of agriculture, fisheries and food, and the scottish home and health department. suggestions are made in this report to st ...	1971	4326247
electrophoretic analysis of ribosomal and viral ribonucleic acids with a simple technique for slicing low-concentration polyacrylamide gels.	the electrophoretic mobilities of ribosomal ribonucleic acids (rna) from cultured mammalian (hela, vero, mdbk), avian (chick embryo), and bacterial (escherichia coli) cells, and rna species extracted from selected viruses (sindbis, polio, tobacco mosaic, sendai) were compared, employing a simple, inexpensive technique for slicing low-concentration polyacrylamide gels. the procedure provides for rapid fractionation of gels used for characterization of rna, incorporating extrusion and serial secti ...	1971	4331767
[detection of e. coli in cow's milk].		1971	4942596
characteristics of antimicrobial resistance of escherichia coli from animals: relationship to veterinary and management uses of antimicrobial agents.	five-hundred fifty-five (555) isolates of escherichia coli were obtained from fecal specimens of a representative number of animals from five farms in the united states. antibiotic exposure of the selected herds was determined by an epidemiological survey of these farms. the incidence of multiple resistance in the e. coli isolates was higher in herds exposed to continuous feeding of antimicrobial agents (84.8%) than in a herd not receiving antimicrobials (15.7%). the most common resistance confi ...	1971	4943278
[disinfection in colibacteriosis of small cattle].		1971	4946542
[microflora of the uterus in postpartum cows].		1971	4946545
uridinediphosphogalactose-4-epimerase deficiency in salmonella typhimurium and its correction by plasmoid-borne galactose genes of escherichia coli k-12: effects on mouse virulence, phagocytosis, and serum sensitivity.	the synthesis of smooth lipopolysaccharide (lps) in relation to mouse virulence and resistance to serum bactericidal activity in vitro and to rapid intravenous clearance in vivo was studied in salmonella typhimurium by using a virulent [median lethal dose (ld(50)) = 10(2)], smooth, and genetically marked strain, a uridinediphosphogalactose epimerase-deficient mutant of it which was, therefore, rough, and a derivative of the mutant made smooth again by acquisition of the galactose-positive genes ...	1971	4949486
antibacterial activity of dihydro-1,3-oxazine derivatives condensed with aromatic rings in positions 5,6.	1. the antibacterial activity in vitro of dihydro-1,3-oxazine derivatives with aromatic rings condensed in positions 5, 6 was examined.2. of more than thirty compounds examined, two (t 615 and t 638) showed marked activity against various strains of mycobacterium tuberculosis at concentrations below 2 mug/ml.3. these two compounds also showed marked activity against escherichia coli, clostridium pneumoniae and salmonella typhi.4. both showed marked activity in vivo against tuberculosis produced ...	1971	5003353
escherichia coli agglutinins in cow serum, colostrum and the nursing calf.	immunochemical properties of escherichia coli o antibodies present in bovine serum and colostrum were investigated. dam and calf serum samples plus colostral whey samples were fractionated by gel filtration, and the 7s and 19s fractions isolated. antibody activity against the o antigens of four recognized e. coli bovine pathogens was determined by the indirect hemagglutination test on the whole serum and colostral whey samples and the 7s and 19s fractions thereof. mercaptoethanol reduction was u ...	1970	4248443
volatile fatty acids and the inhibition of escherichia coli growth by rumen fluid.	concentrations of volatile fatty acids (vfa) normally found in bovine rumen fluid inhibited growth of escherichia coli in antibiotic medium 3. acetic, propionic, and butyric acids each produced growth inhibition which was markedly ph-dependent. little inhibition was observed at ph 7.0, and inhibition increased with decreasing ph. a combination of 60 mumoles of acetate, 20 mumoles of propionate, and 15 mumoles of butyrate per ml gave 96, 69, and 2% inhibition at ph 6.0, 6.5, and 7.0, respectively ...	1969	4886864
comparative studies of antigens from mycoplasma mycoides and escherichia coli.	extracts of sonically disrupted mycoplasma mycoides and escherichia coli were fractionated by sucrose density gradient centrifugation. the presence of antigen in each of the fractions was determined by complement-fixation and agar-gel diffusion precipitin tests, in which cow, pig, and rabbit anti-m. mycoides sera and rabbit anti-e. coli serum were used. fractions of m. mycoides, with a buoyant density of 1.225 or lower, fixed complement with cow and pig anti-m. mycoides sera. these fractions als ...	1968	4967193
in vitro and in vivo characterization of pyocin.	pyocin, a bacteriocin obtained from lysates of ultraviolet-induced cultures of pseudomonas aeruginosa was characterized in vitro and in vivo after 1,000-fold purification by chemical, column, and differential centrifugation procedures. electron micrographs of negatively stained pyocin preparations contained rod-shaped particles which resembled the contractile tail protein of the t-even phages of escherichia coli. although two separate and distinct pyocin fractions were eluted from diethylaminoet ...	1967	4960899
evaluation of t3 coliphage injuries and efficacy of selected materials in preventing them.	a procedure was developed to analyze the inactivation of coliphage t3 during freeze-drying and subsequent rehydration. the amount of gross disruption of the phage as compared with the amount of phage remaining intact was evaluated by cesium chloride density gradient centrifugation. the amount of phage material able to adsorb to host cells and the residual infectivity after the drying were also evaluated. these analyses made it possible to determine the amount of phage material (i) degraded to pr ...	1967	4860532
[the properdin level in cattle during immunization and hyperimmunization with escherichia coli, pasteurella and tetanus toxin].		1965	5321201
galactosidase action on human blood group b active escherichia coli and on red cell substances.	the blood group b specificity of escherichia coli o(86) antigen, a b specific oligosaccharide fraction from it, and the b activity of a preparation of an "infectious mononucleosis receptor" from cattle erythrocytes are destroyed by alpha-galactosidase from the coffee bean, as demonstrated for the e. coli antigen by three methods. blood group h(o) activity appears simultaneously. galactose is the only hexose released from all three substances.	1964	14202031
escherichia coli associated with colostrum-free neonatal pigs raised in isolation.	escherichia coli 08 was the most frequent coliform isolated from the blood and liver of morbid and dead neonatal, colostrum-free piglets raised under extremely sanitary conditions. this strain accounted for 67 per cent of the typable e. coli. the next most numerous strain occurred at a frequency of 6 per cent. hence, e. coli 08 was considered the main coli enteropathogen in our experimental, isolated environment. in random samples of the feces of healthy and diarrhetic neonatal piglets, 24 per c ...	1962	14463349
studies on the metabolic function of branched-chain volatile fatty acids, growth factors for ruminococci. i. incorporation of isovalerate into leucine.	allison, milton j. (dairy cattle research branch, u. s. department of agriculture, beltsville, md.), m. p. bryant, and r. n. doetsch. studies on the metabolic function of branched-chain volatile fatty acids, growth factors for ruminococci. i. incorporation of isovalerate into leucine. j. bacteriol. 83:523-532. 1962.-ruminococcus flavefaciens strain c94, a cellulolytic rumen bacterium, requires either isobutyrate or isovalerate for growth. the organism was grown in the presence of c(14)-labeled i ...	1962	13860621
studies of the bactericidal action of phagocytin.	the bactericidal activity of phagocytin on gram-negative enteric bacilli is influenced by the reaction of the medium; the more acid the environment, the more marked is the activity. phagocytin exerts approximately the same action whether citrate, acetate, or phosphate salts are used as buffer, and the addition of glucose, casein hydrolysate, or cation binding agents does not produce notable change. although proteins in general have but little effect, the inclusion in the medium of a high concent ...	1956	13319581
cloning and sequence analysis of wild argali isg15 cdna.	the complete coding sequence of wild argali isg15 cdna was generated by rapid amplification of cdna ends. the isg15 cdna was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kda protein composed of 157 amino acids. its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of isg15cdna from ovis aries (accession no. nm001009735.1), capra hircus (accession no. hq329186.1), bos taurus (accession no. bc102318.1), bubalus bubalis (accession no. hm543269.1) ...	0	25049988