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a pilot study of in vivo immediate tissue contraction with co2 skin laser resurfacing in a live farm pig.it has been suggested that tissue ablation, collagen shrinkage, and new collagen deposition contribute to the clinical outcome of laser skin resurfacing.199910594596
effect of challenge dose and route on porcine reproductive and respiratory syndrome virus (prrsv) infection in young swine.porcine reproductive and respiratory syndrome virus (prrsv) is perceived to be highly infectious because of the rapid spread of the virus through populations of domestic swine throughout the world. however, no information has been published on the minimum infectious dose of prrsv and the effect of challenge dose on clinical response. in this experiment, ten groups of pigs (n = 3 per group) were inoculated with one of five different quantities (10(1)-10(5) fluorescent foci units per millilitre) o ...199910596410
introducing fluorine-18 fluoromisonidazole positron emission tomography for the localisation and quantification of pig liver hypoxia.fluorine-18 labelled fluoromisonidazole ([18f]fmiso) has been shown to accumulate in hypoxic tissue in inverse proportion to tissue oxygenation. in order to evaluate the potential of [18f]fmiso as a possible positron emission tomography (pet) tracer for imaging of liver tissue hypoxia, we measured the [18f]fmiso uptake in 13 domestic pigs using dynamic pet scanning. hypoxia was induced by segmental arterial hepatic occlusion. during the experimental procedure the fractional concentration of insp ...19999933343
seroprevalence of trichinella infection in domestic swine based on the national animal health monitoring system's 1990 and 1995 swine surveys.swine sera collected by the us department of agriculture's center for animal health monitoring during 1990 and 1995 was tested for antibodies to trichinella spiralis using an enzyme immunoassay. from a total of 3048 sera collected from lactating sows in 1990, five sera tested positive for a prevalence of 0.16%. from a total of 7987 sera collected from both finishing pigs and gestating sows in 1995, one serum was positive for a prevalence of 0.013%. responses to questionnaires administered at the ...19999950336
adventitial angiogenesis early after coronary angioplasty : correlation with arterial remodeling.the spatial correlation between arterial wall microvessels and the accumulation of atherosclerotic plaque is well documented. the role of these microvessels in the development of primary and restenotic lesions is not known. to investigate the effect of interventional procedures on arterial wall microvessels, we studied the adventitial microvascularity of porcine coronary arteries subjected to angioplasty. twenty-two juvenile domestic swine were subjected to single or repeated (double) balloon an ...19999974402
trichinellosis in the united states, 1991-1996: declining but not gone.since the u.s. public health service began recording statistics on trichinellosis in 1947, the number of cases reported by state health departments has decreased steadily. in the late 1940s, health departments reported an average of 400 cases and 10-15 deaths each year. from 1991 to 1996, the period covered in this report, three deaths in 230 cases were reported to the centers for disease control and prevention (an average of 38 cases per year), including 14 multiple case outbreaks from 31 state ...19999988325
filtered versus unfiltered technetium sulfur colloid in lymphatic mapping: a significant variable in a pig model.lymphatic mapping with sentinel node biopsy is becoming a standard diagnostic test for melanoma and is being extensively investigated for use with other soft tissue tumors. both filtered and unfiltered technetium sulfur colloid (tc 99) have been used for preoperative lymphoscintigraphy, as well as intraoperative lymphatic mapping, and it is not clear if one is preferable over the other. the purpose of this study was to compare these two preparations to determine whether the form of tc 99 used af ...199910030419
the effect of wiping on skin resurfacing in a pig model using a high energy pulsed co2 laser system.the impact of wiping in laser skin resurfacing has not been systematically studied.199910037508
oral immunisation of swine with a classical swine fever vaccine (chinese strain) and transmission studies in rabbits and sheep.seven experiments including a total of 47 pigs, 11 wild boars, 26 rabbits, 10 hares and 16 sheep were carried out to assess the efficacy, safety and transmission of the chinese vaccine strain of the classical swine fever virus (csfv) administrated by the oral route. within 3 weeks after oral vaccination, a clear seroconversion occurred in the pigs. six weeks after vaccination, vaccinated pigs were fully protected against a virulent challenge. the c-strain was not isolated from tonsils, spleen, l ...199910063532
hepatic cryosurgery via minilaparotomy in a porcine model: an alternative to open cryosurgery.cryosurgery of liver lesions is becoming increasingly accepted for the ablation of liver tumors. attempts at laparoscopic cryosurgery have been very limited and often need to be converted to open laparotomy due to the complexity of the procedure.199910064758
sutureless small bowel anastomoses: experimental study in pigs.to evaluate a new technique for experimental anastomosis with fibrin glue, and to compare the results with those of stapled and one-layer sutured anastomosis.199910069636
erv-l elements: a family of endogenous retrovirus-like elements active throughout the evolution of mammals.we have previously identified in the human genome a family of 200 endogenous retrovirus-like elements, the herv-l elements, disclosing similarities with the foamy retroviruses and which might be the evolutionary intermediate between classical intracellular retrotransposons and infectious retroviruses. southern blot analysis of a large series of mammalian genomic dnas shows that herv-l-related elements-so-called erv-l-are present among all placental mammals, suggesting that erv-l elements were al ...199910074184
plesiomonas shigelloides and its serovars in animals in the czech republic--region moravia.in the period of 1994-1997, 4,552 samples of sectional, clinical material and environment were examined for the presence of plesiomonas shigelloides. in 55 cases (1,21%) plesiomonas shigelloides was isolated. within 55 isolated strains, 26 serovars have been successfully identified. among other secured cases was plesiomonas shigelloides isolated in pig (sus scrofa domestica), mouse mus musculus), binturong (arctictis binturong) and raccoon (pocyon lotor). in mandarin duck (aix galericulata) the ...199910084023
vascular endothelial growth factor in porcine coronary arteries following balloon angioplasty.the present study was a preliminary inquiry into the presence of vascular endothelial growth factor (vegf) in a model of coronary artery response to injury. we examined domestic pigs who had received a diet enriched in saturated fat and cholesterol and undergone balloon angioplasty of one or more coronary arteries. immunohistochemical analysis of the coronary arteries 2 months after injury revealed the presence of vegf distributed throughout the media and neointima of the angioplasty lesions and ...199910084675
[effects of octreotide (somatostatin analog sms 201-995) on superior mesenteric artery blood flow in swine. an experimental study using doppler color ultrasonography].the effect of octreotide on splanchnic haemodynamics was investigated in 25 domestic pigs by mean of us-colordoppler. in a blind-operator design the superior mesenteric artery (sma) blood flow was evaluated before and after i.m. injection of 5, 10, 20, 25 micrograms/kg or of an equivalent volume of saline solution. seven serial measurements were taken every 10 min in each pig excluding the higher and the lower values. the administration of 5 and 10 micrograms/kg determined a rapid and stable (3 ...199910097449
confirmed quantitative trait loci for fatness and growth on pig chromosome 4.quantitative trait loci (qtls) with large effects on fatness and growth have been identified previously on pig chromosome 4 in an intercross between the european wild pig and large white domestic pigs. two f2 sows, heterozygous for the actual chromosome region, were backcrossed to a large white boar, and two backcross (bc1) boars were in turn backcrossed to large white/landrace sows. one of the boars was heterozygous for an intact wild pig-derived qtl region, whereas the other carried a recombin ...199910098263
effects of overlap and pass number in co2 laser skin resurfacing: a study of residual thermal damage, cell death, and wound healing.newer co2 laser systems incorporating short pulse and scanning technology have been used effectively to resurface the skin. as the number of resurfacing cases has increased, hypertrophic scarring has been reported more commonly. previous dermabrasion and continuous wave co2 studies have suggested that depth of injury and thermal damage are important predictors of scarring for a given anatomic region. to determine whether rapid overlapping of laser pulses/scans significantly altered wound healing ...199910100647
melatonin receptors in neonatal pig brain and pituitary gland.summer infertility remains a major problem in domestic pigs. it has been proposed that sows which display this trait are inherent seasonal breeders. the influence of photoperiod on domestic pigs has been difficult to ascertain as significant diurnal fluctuations in blood levels of the pineal hormone, melatonin, which provide a direct neuroendocrine transduction of the ambient photoperiod in other species, remain questionable in adult pigs. to investigate whether the pig is potentially receptive ...199910102759
[comparison of laboratory diagnostic methods for the detection of infection with the virus of classical swine fever in the early inspection phase: an experimental study].virus isolation in the pk-15 cell culture, two commercial antigen elisas, reverse transcriptional-polymerase chain reaction (rt-pcr), and flow cytometry have been evaluated to detect viremic pigs in the early period of classical swine fever virus (csfv) infection. domestic pigs were experimentally inoculated with the virulent csfv strain 'alfort 187' and two field isolates. csfv isolation and rt-pcr were found to be the most sensitive methods for the detection of highly virulent csfv in the earl ...199910189722
antilithiasic effect of beta-cyclodextrin in lpn hamster: comparison with cholestyramine.beta-cyclodextrin (bcd), a cyclic oligosaccharide that binds cholesterol and bile acids in vitro, has been previously shown to be an effective plasma cholesterol lowering agent in hamsters and domestic pigs. this study examined the effects of bcd as compared with cholestyramine on cholesterol and bile acid metabolism in the lpn hamster model model for cholesterol gallstones. the incidence of cholesterol gallstones was 65% in lpn hamsters fed the lithogenic diet, but decreased linearly with incre ...199910191297
vascularization and cartilage mineralization of the thyroid cartilage of munich minipigs and domestic pigs.thyroid cartilages of munich minipigs and domestic pigs were investigated by polychrome sequential labeling, radiography, intravascular injections, histologic examination and scanning electron microscopy in order to gain further insight into the process of vascularization and cartilage mineralization. the relationship between vascularization and cartilage mineralization has only been studied in chondroepiphyses of long bones. vessels branch off the perichondrial vascular network and enter parts ...199910195303
vasopressin improves vital organ blood flow after prolonged cardiac arrest with postcountershock pulseless electrical activity in pigs.although a benefit of vasopressin when compared with epinephrine was shown during cardiopulmonary resuscitation (cpr) after a short duration of ventricular fibrillation cardiac arrest, the effect of vasopressin during prolonged cardiac arrest with pulseless electrical activity is currently unknown.199910199526
does desmopressin reduce bleeding in liver surgery? an experimental study on pigs.the main problem with liver surgery is to control intraoperative bleeding. bleeding together with postoperative infection and liver failure are the most common causes of postoperative morbidity and mortality in liver surgery. hospitals with experience of liver resection, report postoperative mortality rates of 3.2%-14.3%. improved results in liver surgery have been made by refinement of surgical methods and modern techniques. desmopressin acetate (octostim) reduces bleeding time by activating pl ...199910218138
prevalence of toxoplasma gondii antibodies in sera of domestic pigs and some wild game species from zimbabwe.serum samples of domestic pigs (sus scrofa), elands (taurotragus oryx), sable antelopes (hippotragus niger), warthogs (phacochoerus aethiopicus), bushpigs (koiropotamus [potamochoerus] koiropotamus), white rhinos (ceratotherium simus), african buffalos (syncerus caffer), wildebeest (connochaetas taurinus), and african elephants (loxodonta africana) from zimbabwe were tested for toxoplasma gondii igg antibodies by the modified agglutination test (mat) with whole formalized tachyzoites and mercapt ...199910219323
cardiopulmonary effects of the laparoscopic pneumoperitoneum in a porcine model of adult respiratory distress syndrome.increasingly, laparoscopy is being used in critically ill patients in whom there is a question of intra-abdominal sepsis. we examine the cardiopulmonary effects of laparoscopy in a porcine model of adult respiratory distress syndrome (ards).199910219857
effect of porcine reproductive and respiratory syndrome virus (prrsv) on alveolar lung macrophage survival and function.porcine reproductive and respiratory syndrome virus (prrsv) recently emerged as an important cause of reproductive disorders and pneumonia in domestic pigs throughout the world. acute cytocidal replication of prrsv in alveolar lung macrophages causes the acute pneumonia; however, it remains largely unresolved whether there may also be a predisposition to longer-term local immunodeficiency in the prrsv-convalescent lung. we applied various flow cytometric techniques to study the interplay between ...199910223319
prevalence and risk association for trichinella infection in domestic pigs in the northeastern united states.to determine trichinella infection in a selected group of farm raised pigs, 4078 pigs from 156 farms in new england and new jersey, employing various management styles, were selected based on feed type (grain, regulated waste, non-regulated waste). the number of pigs bled from each farm were based on detecting infection assuming a 0.05 prevalence rate. serum was tested by enzyme-linked immunoassay for antibodies to trichinella spiralis. seropositive pigs were tested by digestion at slaughter (wh ...199910223350
mr-guided percutaneous nephrostomy of the nondilated upper urinary tract in a porcine model.the purpose of this study was to assess the safety and feasibility of mr-guided percutaneous nephrostomy of the nondilated contrast-enhanced upper urinary tract in a porcine model.199910227492
evaluation of ureteric contraction: a comparison among ring, spiral-cut and longitudinal segments.to determine the optimal contractile response of isolated ureters to inflammatory mediators and neurotransmitters by evaluating four common methods of ureteric suspension.199910233460
prevalence of toxoplasma gondii infection in domestic pigs in the new england states.to determine regional prevalence of infection with toxoplasma gondii, 1897 pigs from 85 farms in five new england states were tested using a modified direct agglutination test. sera were diluted 1:25 and a titer at this dilution was indicative of t. gondii infection. farm management questionnaires were completed at the time of blood collection and were used to develop descriptive statistics on farms tested and to determine measures of association for risk factors for the presence of t. gondii-se ...199910321584
electrocardiographic prediction of the success of cardiac resuscitation.to identify a method for predicting the success or failure of a defibrillatory shock such as to avoid potentially detrimental interruptions of cardiopulmonary resuscitation (cpr). such a method would also guide more optimal programming of automated external defibrillators.199910321659
ct-monitored percutaneous cryoablation in a pig liver model: pilot study.to determine the safety and feasibility of percutaneous cryoablation with computed tomographic (ct) guidance in a pig liver model.199910352592
detection of glycosidic residues in carpal glands of wild and domestic pig revealed by basic and lectin histochemistry.carpal glands are compound tubuloalveolar glands, located on the medial surface of the carpus. this study was carried out on samples from carpal glands of adult wild and domestic pigs of both sexes. we elucidated the glycosidic composition of carpal gland secretion in situ using traditional histochemical methods and lectin histochemistry. some secretory cells exhibited an intense reaction with pas in both wild and domestic pigs. lectin histochemistry showed differences in the localization and co ...199910363109
[linkage of a porcine esterase d locus with a lethal factor].this work is devoted to a linkage study of the gene controlling esterase d and a lethal factor in domestic pig. segregation for two alleles esdb, which originated from populations of miniature swine and pigs of a large white breed, and the effect of these variants on the number of offspring in a litter were compared. the results showed a linkage of locus esd with a lethal factor. this lethal factor causes the mortality of homozygotes and half of the heterozygotes in a litter, which depends on th ...199910368786
african swine fever virus: a b cell-mitogenic virus in vivo and in vitro.the two major characteristics of pathogenesis in african swine fever virus (asfv) infections of domestic pigs are massive b-cell apoptosis and haemorrhage. the effects of asfv on porcine b cells have therefore been systematically examined in vivo, by using virus-infected pigs and scid-beige mice reconstituted with porcine bone marrow, and in vitro, by using porcine b-cell lines and b cells from normal and asfv-infected pigs. secretion of porcine ig was stimulated by asfv both in vivo and in bone ...199910374963
characterization of porcine and ovine oesophagostomum spp. by isoenzymatic patterns and restriction-fragment-length polymorphisms (rflps).four different morphological and biometrical populations of oesophagostomum have been identified, using classical taxonomy methods, from sus scrofa domestica (pigs): o. dentatum (od), o. quadrispinulatum (oq), o. granatensis (og), and a fourth population, including individuals with morphological and biometric parameters overlapping these three species that were clasified as oesophagostomum sp. the g6pd and mdh isoenzymatic patterns did not discriminate between the three species, while gpi showed ...199910379817
morphological study of mast cell localization in the wall of the proximal tubule in the domestic swine kidney.morphological studies to localize mast cells in the wall of the convoluted and straight parts of the proximal tubules of the domestic swine kidney were performed. kidney perfusion was carried out in 8-month-old pigs and semi-thin and ultrathin sections were prepared after a routine treatment. a light microscopic study showed that mast cells were not frequently found. most were localized in the wall of the proximal convoluted tubule, while in the proximal straight tubule wall they were fewer in n ...199910386001
responses of single taste fibers and whole chorda tympani and glossopharyngeal nerve in the domestic pig, sus scrofa.whole nerve, as well as single fiber, responses in the chorda tympani proper (ct) and glossopharyngeal (ng) nerves of 1- to 7-week-old pigs were recorded during taste stimulation. in the ct acids and in the ng bitter compounds gave the largest responses. both nerves exhibited large responses to monosodium glutamate (msg), msg with guanosine 5'-monophosphate (gmp) and msg with inositine 5'-monophosphate (imp) as well as to glycine, xylitol, sucrose, fructose and glucose. alitame, aspartame, betai ...199910400449
statistical method for characterization of hypertrophic cardiomyopathy by use of morphologic and pathologic measurements in pigs (sus scrofa domestica).hypertrophic cardiomyopathy (hcm) is characterized by symmetric or asymmetric hypertrophy of the left and/or right ventricle. morphologic and pathologic indices (mi and pi) of hearts were established for classification of hcm in pigs.199910403442
temporal differences in the responses of the pituitary adrenocortical axis, the sympathoadrenomedullar axis, heart rate, and behaviour to a daily repeated stressor in domestic pigs.in this study we examined responses of domestic pigs (sus scrofa domestica) to a daily repeated stressor. in particular, we focused on differences in temporal changes across treatment repetition between the pituitary adrenocortical axis, the sympathoadrenomedullar axis, heart rate, and behaviour. to induce a stress response, we separated eight castrated male pigs from their group mates visually and auditorily for 1 h on 10 successive days. overall, enhanced plasma levels of cortisol, acth, and a ...199910405105
laparoscopic renal cryoablation: acute and long-term clinical, radiographic, and pathologic effects in an animal model and application in a clinical trial.to evaluate renal cryosurgery by studying the feasibility of laparoscopic delivery and the radiographic characteristics and histopathologic effects in a porcine model using different freeze cycles. on the basis of the results, a clinical trial of laparoscopic cryosurgical ablation in select patients with clinical stage t1 renal tumors was started.199910405898
laparoscopic replacement of urinary tract segments using biodegradable materials in a large-animal model.we elected to evaluate laparoscopic segmental bladder and ureteral replacement with free biodegradable graft materials in a large-animal model.199910405899
antifibrinolytic additives to fibrin glue for laparoscopic wound closure in urinary tract.fibrinolytic activity of urine may rapidly degrade fibrin glue used in the urinary tract, thereby limiting tissue adhesion. the goals of this study were to verify the ability of antifibrinolytic agents to delay the degradation of fibrin glue in the urinary tract and to assess the results of this delay on subsequent wound healing.199910405907
the differentiation of traumatic and heat-related fractures in burned bone.interpretations of antemortem and perimortem trauma are complicated when dealing with cases involving extreme exposure to fire. this investigation attempts to discern the signatures of perimortem trauma from heat related trauma. femora of domestic pig, sus scrofa, with minimal soft tissue and articulated patellae were subjected to varying traumatic forces. skeletal elements were impacted with blunt and sharp forces, cut with varying instruments, subjected to torsional forces of shot. bones were ...199910408102
decreased cerebrospinal fluid absorption during abdominal insufflation.intracranial pressure (icp) is known to rise during induced co(2) pneumoperitoneum. this rise correlates with an increase in inferior vena caval pressure; therefore, it is probably associated with increased pressure in the lumbar venous plexus. branches of this plexus communicate with arachnoid villi in the lumbar cistern and the dural sleeves of spinal nerve roots-areas where cerebrospinal fluid (csf) absorption to normally takes place. the increased venous pressure in this area may impede csf ...199910430688
beneficial effects of leukocyte-depleted blood and low-potassium dextran solutions on microvascular permeability in preserved porcine lung.modified euro-collins (ec) solution, a crystalloid intracellular-type solution, has been commonly used for pulmonary preservation. several experimental studies have shown the advantages of using extracellular colloid-based solutions. the aim of this study was to compare the quality of preservation of two extracellular colloid solutions, leukocyte-depleted blood (bl) and low-potassium dextran (lpd) solutions, with that of ec solution. lungs of 22 domestic pigs were flushed and preserved with ec ( ...199910430747
trichinella nativa in sylvatic wild boars.of 17 trichinella isolates from domestic pigs and wild boars (sus scrofa) in regions where trichinella nativa is widespread among sylvatic animals, two wild boars from estonia were found to be naturally infected with this trichinella species. the other 15 animals were infected with trichinella spiralis. trichinella nativa is tolerant to freezing when in the muscles of carnivores. the biological characteristics and temperature tolerance of this species in swine need to be further investigated if ...199910431373
central haemodynamics in experimental acute pancreatitis.to investigate central haemodynamics in severe and mild acute pancreatitis in pigs.199910433147
resting rectal temperature of vietnamese potbellied pigs.to determine resting rectal temperatures of vietnamese potbellied pigs.199910434970
the effect of morphine on melatonin secretion in the domestic pig. in vivo and in vitro study.up till now the results of performed investigations suggest the involvement of opioids in the regulation of the pineal gland activity in mammals. on the other hand, they show the existence of large interspecies differences in the presence of opioid peptides in the pineal gland and in the effects of opiates on the melatonin secretion. the aim of the present work was to study the influence of morphine on the melatonin secretion in the domestic pig. morphine (about 2.5 mg/kg) was given intravenousl ...199911462111
clinical and experimental study on regional administration of phosphorus 32 glass microspheres in treating hepatic carcinoma.aim:to study the therapeutical effectiveness, dosage range and toxic adverse effects of domestic phosphorus 32 glass microsphere and evaluate its clinical significance.methods:i.fifty two balb/c tumor bearing male nude mice were allocated into treatment group(n = 38) and control group(n = 14). in the former group different doses of (32) p-gms were injected into the tumor mass, while in the latter (31)p-gms or no treatment was given. the experimental animals were sacrificed in batches, and then t ...199911819498
influence of age on the electrocardiographic waves in taiwanese lan-yu miniature pigs.the purpose of the study reported here was to determine baseline information of the electrocardiogram (ecg) and the influence of age on cardiac function in taiwanese lan-yu (tly) miniature pigs. non-anesthetized tly minipigs (from birth to 6 months of age) were placed on a webbed stanchion, simulating a standing position, for acquisition of an ecg, using six limb leads (i, ii, iii, avr, avl, and avf), with each as a single-channel ecg recorder. the p and t waves obtained from leads i, iii, and a ...199912086414
detection of low-virulent classical swine fever virus in blood of experimentally infected animals: comparison of different methods.the effectiveness of virus isolation, commercial antigen enzyme-linked immunosorbent assay (elisa), reverse transcriptase-polymerase chain reaction (rt-pcr), and flow cytometry in detection of a low-virulent classical swine fever virus (csfv) in blood in the early period of infection was evaluated. domestic pigs at the age of 6-8 weeks and young wild boars were inoculated with a low-virulent field isolate of csfv originating from a wild boar. this virus induced serious clinical reaction in only ...199910825927
mitochondrial genetic variation in chinese pigs and wild boars.the mitochondrial dnas (mtdnas) from 30 pig breeds (29 chinese native breeds and 1 european breed) and wild boars were investigated for restriction fragment length polymorphisms (rflps) to determine the phylogenetic relationships and genetic diversity among pig breeds and wild boars. of the 24 enzymes used, 8 (avai, bcli, bglii, ecori, ecorv, scai, stui, and xbai) detected polymorphisms. by combining the cleavage patterns for each enzyme, 108 individuals were sorted into eight mtdna mitotypes. t ...199910690429
anatomy and histology of the babirusa (babyrousa babyrussa) stomach.stomachs from six adult and one 12-month-old babirusa (babyrousa babyrussa) were collected from zoological gardens. the babirusa stomach was larger than that of the domestic pig (sus scrofa), and possessed a large diverticulum ventriculi. its gastric glands were confined to a small, easily identifiable unit at the end of the corpus ventriculi, and the connections between the different stomach parts were wide and unrestricted, with the exception of the well-defined connection between the divertic ...199910656148
the influence of systemic growth hormone administration on the healing time of skin graft donor sites in a pig model.a more rapid healing of skin graft donor sites has often been observed during ultimoratio therapies with growth hormone in adults who have suffered extremely severe burns. the purpose of this animal experimental study was to examine the influence of systemic growth hormone administration on the healing time of skin graft donor sites under standardized conditions in pigs. the animals were 14 (7 experimental and 7 control) male, sexually mature, german domestic pigs, in which 30 skin graft donor s ...199910654690
matrix metalloproteinases (mmps) are required for re-epithelialization of cutaneous wounds.matrix metalloproteinases (mmps) are neutral zinc-dependent endopeptidases with substrate specificity for most extracellular matrix molecules. mmps participate in physiological and pathological biological processes. in wound repair, several mmps are upregulated in migrating epithelium although the biological significance of their presence is unknown. to elucidate the role of mmps in epithelial migration of cutaneous wounds, a broad-spectrum synthetic mmp inhibitor (gm 6001, 10 mg/ml) was applied ...199910638331
high-resolution magnetic resonance coronary angiography of the entire heart using a new blood-pool agent, nc100150 injection: comparison with invasive x-ray angiography in pigs.recent developments of novel magnetic resonance intravascular contrast agents with low t1 in blood and a long intravascular half-life will rapidly position magnetic resonance coronary angiography (mrca) at the threshold of clinical application. this article describes the use of one such intravascular contrast agent for noninvasive coronary angiography and comparison with routine invasive x-ray angiography. six domestic farm pigs with an artificial stenoses at the left circumflex were studied. nc ...199911550346
toxicology of intrahepatic arterial administration of interventional phosphorus-32 glass microspheres to domestic pigs.to evaluate the toxic response to intrahepatic arterial administration of radioactive phosphorus-32 glass microspheres (32p-gms) in domestic pigs.199911601259
photoreceptor density of the domestic pig retina.the spatial distribution and densities of photoreceptors in seven whole-mounted porcine retinas were studied and maps illustrating photoreceptor topography were constructed. total photoreceptor densities ranged from to 83 000 to 200 000 cells/mm2, with a mean of 138 500 cells/mm2. cone densities ranged from 39 000 (area centralis) to 8500 cones/mm2 (peripherally), with a mean of 16 400 cones/mm2. rod:cone ratios ranged from 3:1 centrally to 16:1 peripherally, with a mean ratio of 8:1. averaged p ...199911397262
genetic evidence for the role of gdp-mannose in plant ascorbic acid (vitamin c) biosynthesis.vitamin c (l-ascorbic acid; asa) acts as a potent antioxidant and cellular reductant in plants and animals. asa has long been known to have many critical physiological roles in plants, yet its biosynthesis is only currently being defined. a pathway for asa biosynthesis that features gdp-mannose and l-galactose has recently been proposed for plants. we have isolated a collection of asa-deficient mutants of arabidopsis thaliana that are valuable tools for testing of an asa biosynthetic pathway. th ...199910097187
[pharmacognosy of hog nail of sus scrofa domestica brisson].this paper reports the characteristics of microscopic and physicochemical identifications, as well as the determination of amino acids and inorganic elements in unguis suillus of the crude drug hog nail of sus scrofa domestica. the results show that the protein content of unguis suillus amounts to 80.65%. the amino acids in the drug consist of 7 essential amino acids and 9 nonessential amino acids. the main inorganic elements are na, mg, al, si, p, s, cl, k, ca, fe and zn.199811601295
[study on the replacement of the portal vein of pig with autogeneous-peritoneal tube].to investigate the possibility of applying autogeneous peritoneal tube in the portal vein reconstruction.199811367686
bone morphogenetic protein and ceramic-induced osteogenesis.to investigate the relationship between bone morphogenetic proteins (bmp) and calcium phosphate ceramic-induced osteogenesis in soft tissues, in vitro and in vivo experiments were performed. in an in vitro study, the ability of different calcium phosphate ceramics to absorb bovine bmp (bbmp) from a bbmp solution was tested. in vivo studies included immunohistochemical bmp staining before bone formation in the ceramics was detected, and the enhancement of bone formation in calcium phosphate ceram ...199815348928
[experimental study of the architecture of skin following tension traction and wound closure].the experiment performed on domestic pig, was designed to investigate the architecture of the stretching skin and the effect of tension traction on the wound closure. an 7 cm x 3.5 cm was drawn at a standard position. drawing horizontal and vertical lines on the traction area, points of 1 cm apart were tattooed on the lines. a traction force measuring device was used to draw the wound edges together. measurements of the distances between the points were made. an 7 cm x 10 cm wound was made on id ...199810437064
the tissue effects of ultrasound-assisted lipoplasty.the objective of our study was to investigate the effects of ultrasonic energy on tissues, using a porcine model, performed under various instrumental and procedural parameters. domestic pigs were anesthetized and prepared for surgery. an incision was made on the side of the hip randomly assigned to the right or left side. tumescence solution was infiltrated via a blunt tip, small diameter cannula, followed by performance of standard liposuction. on the contralateral side, a similar incision was ...19989655430
african swine fever virus infection of the bushpig (potamochoerus porcus) and its significance in the epidemiology of the disease.warthog (phacochoerus aethiopicus), giant forest hog (hylochoerus meinertzhageni) and bushpig (potamochoerus porcus) are known to be susceptible to infection with african swine fever (asf) virus. little however, is known about the ecology of the disease in the bushpig. this study has shown that the bushpig remains viraemic for between 35 and 91 days following infection during which time it is able to infect the tick vector o. moubata. these ticks were able to transmit the disease to pigs. the vi ...19989659687
morphologic, biometric, and isoenzyme characterization of trichuris suis.trichuris suis isolates were collected from the cecum of sus scrofa domestica (pig) and s. s. scrofa (wild boar). morphology and biometry studies were carried out. morphology studies showed the existence of typical caudal papillae in males of t. suis from wild boars, but no other difference was observed in the biometric parameters (total length, esophageal length, posterior-portion body length, and spicular length) of t. suis isolated from either host. individual extracts were subjected to malat ...19989660144
obliterative lesions in small airways in an immunosuppressed porcine heterotopic bronchial allograft model.we have recently developed a heterotopic large-animal model for research into obliterative lesions in small airways caused by allograft rejection. in this model, the small airways of subcutaneously implanted allografts gradually obliterate, whereas autografts remain patient. twenty lung fragments and 20 segments of bronchi were implanted in domestic pigs weighing 20 kg from non-related donors. the histology of five animals receiving daily cyclosporine a (csa) (10 mg/kg), azathioprine (2 mg/kg) a ...19989665049
nutritional state, growth rate, and morphology after total gastrectomy with restoration of duodenal passage or roux-en-y oesophagojejunostomy with or without a pouch: an experimental study in pigs.to compare the effect of restoration of duodenal continuity by a roux-en-y oesophagojejunostomy with or without a pouch on nutritional state, growth, and morphology after total gastrectomy in pigs.19989667472
detecting gastrointestinal hypoperfusion during cardiac tamponade in pigs: a role for nitric oxide tonometry?to evaluate different techniques and regional approaches for detecting critical reductions in gastrointestinal (gi) perfusion.19989671377
influence of artificial light regimens on sexual maturation and boar taint in entire male pigs.to determine if artificial light regimens could influence sexual maturation and boar-taint factors in entire male pigs, 48 weaned, winter-born crossbred males (52-64 days old) were exposed to either a natural photoperiod (january-june, 60 degrees n, control, n= 16) or to one of two artificial photoperiods (1400 1x) in light-sealed rooms. we exposed the spring group to an increasing artificial photoperiod (january - june, 60 degrees n, n=16) and the autumn group to a decreasing one (july-december ...19989672668
[the situation of classical swine fever in wild boars in the european community and selected aspects of disease transmission].the situation of classical swine fever (csf) in europe is described on the basis of the literature. in the european community, csf is present among wild boars in germany (federal states mecklenburg-western pomerania, brandenburg and lower saxony), in france (northern vosges) and in italy (regio emìlìa romagna in 1997 and sardinia--enzootically infected). infected wild boars are important as a source of infection for domestic pigs in germany. selected aspects of the transmission of csf virus from ...19989674308
systemic and regional pco2 gradients as markers of intestinal ischaemia.we evaluated the response of mixed venous-arterial carbon dioxide (pco2) to severe intestinal ischaemia produced by gradual occlusion of the superior mesenteric artery (sma).19989681782
isolation of putative olfactory receptor sequences from pig nasal epithelium.binding to olfactory receptors is the first step in odorant and pheromonal recognition and discrimination. these receptors constitute one of the most important, although poorly known, families of neuronal receptors. in this study we used degenerated oligonucleotides and a rt-pcr approach to selectively amplify olfactory receptors in the nasal epithelium of the domestic pig sus scrofa. several combinations of oligonucleotide were tested and allowed the isolation of eleven different partial sequen ...19989682823
stent versus endovascular graft healing characteristics in the porcine iliac artery.to evaluate the healing characteristics of stents versus endovascular grafts in the porcine iliac artery.19989684832
transthoracic induction of a hiatal hernia in domestic swine.with the common performance of laparoscopic nissen fundoplication for gastroesophageal reflux disease, there is renewed interest in the pathophysiology and potential histologic consequences of hiatal hernias. however, in vivo model exists that both reliably reproduces the hiatal hernia and is amenable to subsequent laparoscopic repair.19989685543
[mri-guided percutaneous nephrostomy of the contrast-enhanced, nondilated upper urinary tract: initial experimental results].to evaluate technique and practicability of mr-guided percutaneous nephrostomy (mrpcn) in an animal model.19989687955
animal model of acute deep vein thrombosis.to develop an animal model of acute deep vein thrombosis (dvt).19989688802
detection of african swine fever virus in infected pig tissues by immunocytochemistry and in sity hybridisation.the techniques for determining cellular sites of establishment and persistence of african swine fever virus (asfv) were established in susceptible domestic pigs and the resistant african reservoir hosts, the warthog and bushpig. detection, both in vitro and in vivo, was achieved by in situ hybridisation and immunocytochemistry, focusing principally on specific probes for vp73, a major capsid protein. hybridisation of radio-labelled probes for dna and rna was relatively insensitive and time consu ...19989694328
retinoids are endogenous to the porcine blastocyst and secreted by trophectoderm cells at functionally-active levels.retinoic acid and its isomers are the major morphogens in vertebrate development. for mammals, it was previously considered that circulating retinoic acid was recruited from the uterine environment, to influence embryonic differentiation, morphogenesis and development. here we report that retinoic acid is endogenous to the blastocyst of the domestic pig, sus scrofa, as detected by high pressure liquid chromatography. furthermore, using a continuous, normal line of porcine trophectoderm cells, te ...19989694636
is growth influencing diameter and function of laparoscopic tesa hepaticojejunostomy? a comparison of adult minipigs and juvenile domestic swine.current treatment of obstructive jaundice includes endoscopic stenting and open surgical bypass. to combine the advantages of surgical bypass with the minimal invasive approach of endoscopic stenting we developed a laparoscopic technique, transient endoluminally stented anastomosis (tesa). as shown previously, small-diameter anastomoses (e.g., hepaticojejunostomy) by tesa technique can be performed reliably in growing domestic swine. this further preclinical trial was designed to exclude growth ...19989700619
transjugular intrahepatic portosystemic shunt in a porcine model: histologic characteristics at the early stage.the authors attempted to determine the histologic processes that take place during development of stenosis after transjugular intrahepatic portosystemic shunt (tips) creation.19989702265
three-dimensional 87rb imaging of isolated pig hearts: effects of regional ischemia.the aim of this work was to investigate whether 1) rb uptake is reduced in ischemic myocardium, and 2) 87rb three-dimensional (3d) imaging can detect the ischemic area. hearts of domestic pigs (n = 8, 20-30 kg) were perfused retrogradely with krebs-henseleit buffer in a 7-t, 40-cm horizontal bore magnet interfaced with bruker mslx spectrometer. control (c) and ischemic (i) (45 min ligation of the left anterior descending coronary artery (lad)) hearts were loaded with rb+ by perfusion with a rb(+ ...19989702698
technetium-99m-nitroimadazole uptake in a swine model of demand ischemia.nitroheterocycles are electron affinic, lipophilic compounds that are retained in hypoxic tissue. this study was designed to test the hypothesis that 99mtc-5-oxa-amine-oxime nitroimadazole (bms-194796) is retained in ischemic myocardial tissue in a swine model of demand ischemia and that the retained tracer can be imaged in vivo.19989708532
flap flow and cardiac output as functions of pulmonary artery wedge pressure: experimental study in the pig.cardiac output (co) is redistributed during hypovolemia, but it has not been clearly documented how this influences flap perfusion. simultaneous changes in perfusion of a muscle flap and co as modulated by reductions in cardiac filling pressure were compared in a pig model. the hypothesis was that flap flow (ff) would remain relatively constant as co was reduced, according to the frank-starling curve. under general anesthesia, five domestic pigs were utilized. following right carotid artery cann ...19989714037
midazolam-fentanyl-isoflurane anaesthesia is suitable for haemodynamic and fluid balance studies in pigs.the domestic pig is a useful model in certain areas of biomedical research. effective use of this species is often encumbered by lack of reference values in conscious as well as anaesthetized animals. anaesthesia itself influences physiological and biological variables; the anaesthetic technique often affects experimental results. the relationship between anaesthesia and haemodynamics is well characterized in man, but less established in pigs. we studied the effect of midazolam-fentanyl-isoflura ...19989718480
molecular basis for the dominant white phenotype in the domestic pig.the change of phenotypic traits in domestic animals and crops as a response to selective breeding mimics the much slower evolutionary change in natural populations. here, we describe that the dominant white phenotype in domestic pigs is caused by two mutations in the kit gene encoding the mast/stem cell growth factor receptor (mgf), one gene duplication associated with a partially dominant phenotype and a splice mutation in one of the copies leading to the fully dominant allele. the splice mutat ...19989724328
serological and immunohistochemical study of african swine fever in wild boar in spain.a serological and immunohistochemical study of african swine fever was carried out in wild boar killed in seven municipalities in the north of the province of córdoba during two hunting seasons (1991-92 and 1992-93), when the area was affected by the disease. fourteen of 147 wild boar analysed by elisa and immunoblotting had antibodies to african swine fever virus. the immunohistochemical study revealed that four cases (two seropositive and two seronegative) showed immunoreactivity to the anti-v ...19989725185
pulmonary function after one-lung ventilation in newborns: the basis for neonatal thoracoscopy.to maintain good exposure during major video-assisted thoracic surgery it is necessary to deflate completely the ipsilateral lung. however, little is known about the effects of one-lung ventilation (olv) on pulmonary function in newborn patients.19989725400
measurement of adenosine concentration in aqueous and vitreous.the release of adenosine by the ischemic retina may be an initial signal in the development of ischemic macular edema and neovascularization. the levels of adenosine have never been quantified in ocular fluids. in this study, a technique was developed for in vivo measurement of the concentration of adenosine in aqueous and vitreous.19989727417
experimental tricyclic antidepressant toxicity: a randomized, controlled comparison of hypertonic saline solution, sodium bicarbonate, and hyperventilation.we sought to compare the effects of hypertonic sodium chloride solution (hts), sodium bicarbonate solution, and hyperventilation (hv) on severe tricyclic antidepressant (tca) toxicity in a swine model.19989737495
morphologic peculiarities of the renal cortical vasculature connected with blood redistribution in the kidney of the domestic pig.the cortical blood vessels from 2 to 8 month old camborow hybrid pigs and belgium landrace pigs were studied using histologic and semithin sections as well as corrosion casts and cleared preparations of intravascular injection. it was established that the cortical blood vessels of the porcine kidney possess peculiarities that may regulate the redistribution of blood in the cortex. these peculiarities were found in the interlobular arteries, in the efferent arterioles of midcortical nephrons, in ...19989741149
effects of weaning and restraint stress on glucocorticoid receptor binding capacity in limbic areas of domestic pigs.changes in glucocorticoid receptor (gr) binding in different brain areas were investigated in neonatal and adult pigs exposed to psychological stress (weaning) and a physical stressor (repeated snaring). the gr binding was significantly decreased 4 days after weaning in both the hippocampus and the amygdala, but there were no changes in the hypothalamus. repeated snaring of adult pigs resulted in a significant diminished gr binding only in the hippocampus.19989757073
five new cases of reciprocal translocation in the domestic pig.five new cases of reciprocal translocation in the domestic pig are described. three of them, rep(3;5)(p1.3;q2.3), rep(6;13)(p1.5;q4.1) and rep(13;17)(q4.1;q1.1) were found in boars with decreased litter size. the remaining two were identified in animals karyotyped before reproduction: a young boar, rep(4;6)(q2.1;q2.8), and a gilt, rep(2;14)(q1.3;q2.7). a parental origin by inheritance of the translocations was established in cases 1, 4, and 5. a decrease in prolificacy of 43% and 34% was estimat ...19989760870
the toxic mechanism and metabolic effects of atractyloside in precision-cut pig kidney and liver slices.the toxic and cellular metabolic effects of atractyloside, a diterpenoid glycoside, which causes fatal renal and hepatic necrosis in vivo in animals and humans, have been investigated in tissue slices prepared from male domestic pig kidney and liver. precision-cut slices (200 microm thick) were incubated with atractyloside at concentrations of 200 microm, 500 microm, 1.0 mm and 2.0 mm for 3 h at 37 degrees c and changes in lipid profile and pyruvate-stimulated gluconeogenesis investigated. lipid ...19989765068
left ventricular functional improvement after transmyocardial laser revascularization.transmyocardial laser revascularization has been used to treat patients with end-stage coronary artery disease that is not amenable to standard revascularization. although there is evidence of angina relief and quality of life enhancement, there is little information concerning improvement in myocardial contractility. the purpose of this study was to determine whether transmyocardial laser revascularization improves myocardial function in chronically ischemic myocardium.19989768921
diving seals, ischemia-reperfusion and oxygen radicals.the cardiovascular adaptations of seals that contribute to their ability to tolerate long periods of diving asphyxial hypoxia result in episodic regional ischemia during diving and abrupt reperfusion upon termination of the dive. these conditions might be expected to result in production of oxygen-derived free radicals and other forms of highly reactive oxygen species. seal organs vary during dives with respect to the degree and persistence of ischemia. myocardial perfusion is reduced and interm ...19989773490
angiographic and histological evaluation of porcine retinal vascular damage and protection with perfluorocarbons after massive air embolism.we have previously shown that perfluorocarbon emulsions (pfes) reduce the severity of cerebral injury (indicated by infarct, reduced blood flow, and depressed eeg) induced by air embolism during cardiopulmonary bypass (cpb). this study used retinal fluorescein angiography to define the mechanisms of cerebral injury and to determine the efficacy of pfes in cerebral protection. these angiographic findings were correlated to previously reported histologic findings.19989804654
genetic and experimental evidence for cross-species infection by swine hepatitis e virus.prior to the recent discovery of the swine hepatitis e virus (swine hev) in pigs from the midwestern united states, hev was not considered endemic to this country. since swine hev is antigenically and genetically related to human strains of hev, it was important to characterize this new virus further. the infectivity titer of a pool of swine hev in pigs was determined in order to prepare a standardized reagent and to evaluate the dose response in pigs. although the sequence of swine hev varied e ...19989811705
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