Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| monoclonal antibodies multireactive with parasite antigens produced by hybridomas generated from naive mice. | hybridoma clones producing igm naturally occurring (natural) antibodies were generated from naive balb/c mice and characterized for reactivity against parasite antigens. ascites of mice injected with these hybridomas reacted with toxoplasma gondii soluble antigen at levels approximately 1000-10,000 times higher than serum pooled from mice used for generating hybridomas as determined by a conventional enzyme-linked immunosorbent assay. western blot analysis indicated that these monoclonal antibod ... | 1997 | 9364565 |
| entamoeba histolytica: computer-assisted modeling of phosphofructokinase for the prediction of broad-spectrum antiparasitic agents. | pyrophosphate-dependent phosphofructokinase (ppi-pfk) is the rate-limiting glycolytic enzyme found in the pathogenic protists entamoeba histolytica, giardia lamblia, toxoplasma gondii, trichomonas vaginalis, and naegleria fowleri. the enzyme differs significantly from atp-dependent phosphofructokinases found in humans and as such represents an important drug target. current therapy for infections caused by these pathogens is inadequate, especially for children, pregnant women, and the immune com ... | 1997 | 9371084 |
| pleuropulmonary amebiasis. | amebiasis is the third leading parasitic cause of death in the world. approximately 500 million people worldwide are infected with entamoeba histolytica. invasive disease is more common in the immunosuppressed, pregnant women, children, and alcoholics. amebic colitis and liver abscess are the most common intestinal and extraintestinal manifestations of e. histolytica infection. pleuropulmonary complications occur almost exclusively in individuals with a liver abscess. common pleuropulmonary comp ... | 1997 | 9195675 |
| simple differential detection of entamoeba histolytica and entamoeba dispar in fresh stool specimens by sodium acetate-acetic acid-formalin concentration and pcr. | amoebiasis is caused by two distinct species, a pathogenic form (entamoeba histolytica) and a nonpathogenic form (entamoeba dispar), which are morphologically identical. although the distinction between these two species is of great clinical importance, the methods developed for this purpose either are very time-consuming or involve laborious procedures for isolation of the dna. we report here a simple pcr method starting with fresh stool specimen that allows for the sensitive and reliable disti ... | 1997 | 9196177 |
| who/paho/unesco report. a consultation with experts on amoebiasis. mexico city, mexico 28-29 january, 1997. | 1997 | 9197085 | |
| differentiation of entamoeba histolytica from e. dispar facilitated by monoclonal antibodies against a 150-kda surface antigen. | murine monoclonal antibodies (mabs) were produced against an n-octyl-beta-d-glucopyranoside-extracted fraction of trophozoites of entamoeba histolytica hm-1:imss. four of the mabs were reactive with a 150-kda surface antigen characterized by western-immunoblot analysis under nonreducing conditions. when the reactivity of the four mabs with nine reference strains of e. histolytica was examined by an indirect fluorescence antibody test, two of the mabs (eh3015 and eh3023) were found to react with ... | 1997 | 9197389 |
| human amebic liver abscess: expression of intercellular adhesion molecules 1 and 2 and of von willebrand factor in endothelial cells. | liver invasion by amebas with production of amebic liver abscess (ala) is the most common extraintestinal lesion produced by the protozoan parasite entamoeba histolytica. this hepatic damage is characterized by the presence of extensive tissue necrosis. however, little is known about the parasite and host factors involved in the process of tissue damage. during the early establishment of amebas in the liver parenchyma as well as during the extension of the tissue necrosis, parasites interact wit ... | 1997 | 9197402 |
| the tumor necrosis factor alpha-stimulating region of galactose-inhibitable lectin of entamoeba histolytica activates gamma interferon-primed macrophages for amebicidal activity mediated by nitric oxide. | entamoeba histolytica adheres via galactose-lectin (gal-lectin) to human colonic mucins and intestinal epithelial cells as a prerequisite to amebic invasion. native gal-lectin is a protective antigen in the gerbil model of amebiasis. amino acids 596 to 1082 of gal-lectin mediate e. histolytica adherence to target cells and stimulate tumor necrosis factor alpha (tnf-alpha) production by naive murine bone marrow macrophages (bmm). resistance to amebiasis requires an effective cell-mediated immune ... | 1997 | 9199414 |
| cytokine genes are down-regulated when attachment of entamoeba histolytica to ht-29 colon epithelial cells is prevented. | entamoeba histolytica can cause invasive disease by disruption of the intestinal barriers and subsequent lysis of the intestinal cells. adherence to and contact dependent killing of host cells requires the galactose inhibitable lectin. to elucidate the mechanism whereby e. histolytica influences host defence, the authors assessed the change of proinflammatory cytokine genes expressed by colon epithelial cells in response to co-culture with e. histolytica trophozoites and carbohydrates, including ... | 1997 | 9201300 |
| frequency of intestinal parasites in adult cancer patients in mexico. | approximately 28% of the mexican population has intestinal parasites. oncologic patients receiving chemotherapy should have a coproparasitoscopic study to avoid disseminated parasitic infections. the frequency of intestinal parasites, including cryptosporidium and isospora, was evaluated in 100 diarrheic (ds) and 100 formed stools (fs) from adult patients recently diagnosed with cancer, using wet mounts stained with kinyoun, saccharose and znso4 procedures stained with lugol's iodine. seven pati ... | 1997 | 9204612 |
| [amebic colitis and amebic liver abscess--epidemiology and personal case report]. | the large intestine reacts relatively monomorphically to different stimuli. from this differential-diagnostic problems may result. the history of a patient is described which could be pursued clinically over 12 weeks and during the course of which the correction of the diagnosis ulcerative colitis into amoebic colitis was necessary. it is concluded that in every symptomatology of colitis bacterial and parasitologic examinations of the faeces should be performed primarily specially if there is a ... | 1997 | 9206916 |
| prevalence of intestinal parasites in the human population of león, nicaragua. | intestinal parasites appear to be prevalent in nicaragua, which motivated a more extensive prevalence study in which socioeconomic conditions such as degree of crowding, quality of water supply, type of floor and disposal of excretion, were considered. the study was performed on 1267 stool samples from about 8% of the citizens of the city of león. the overall prevalence of intestinal pathogenic parasites among the 1267 individuals was found to be 47.2%. the prevalence of entamoeba histolytica/di ... | 1997 | 9210962 |
| molecular medicine's applications to the developing world: enlightened self interest. | 1997 | 9211411 | |
| preparation of a monoclonal antibody specific for entamoeba dispar and its ability to distinguish e. dispar from e. histolytica. | a monoclonal antibody (mab), mab ed17 (immunoglobulin g2a [igg2a]), prepared against trophozoites of entamoeba dispar saw1734rclar cultured monoxenically with crithidia fasciculata, reacted with 25 of 26 isolates of e. dispar by an indirect fluorescent-antibody test. in contrast, the mab failed to react with any of 20 isolates of e. histolytica or other enteric protozoan parasites. western blot (immunoblot) analysis showed that the molecular mass of the e. dispar antigen recognized by the mab wa ... | 1997 | 9220156 |
| cuda: a dictyostelium gene with pleiotropic effects on cellular differentiation and slug behaviour. | the dictyostelium cuda gene encodes a nucleoplasmic protein that is essential for normal culmination. there are no functionally characterised homologues in other organisms but there is a related gene of unknown function in entamoeba histolytica. the cuda gene is expressed by the prestalk cells that constitute the slug tip (the psta cells), it is not detectably expressed in the band of prestalk cells that lies behind the tip (the psto cells) but it is expressed in the prespore cells. this unusual ... | 1997 | 9226443 |
| invasive amoebiasis in two patients with aids and cytomegalovirus colitis. | homosexual persons or human immunodeficiency virus (hiv)-infected patients frequently excrete cysts of nonpathogenic strains of entamoeba histolytica ("entamoeba dispar"). however, invasive amoebiasis is rare. we report two patients with aids and cytomegalovirus colitis in whom invasive amoebiasis was histologically diagnosed. it is concluded that e. histolytica has to be considered in hiv-infected patients with colitis. | 1997 | 9230408 |
| encystation of entamoeba parasites. | entamoeba histolytica is a protozoan parasite of humans, and the causitive agent of intestinal amebiasis. the disease-causing stage of the parasite is an osmotically sensitive ameboid form, which differentiates into a thick-walled cyst for transmission from person to person. the conditions within the human intestine that induce encystment of the amoeba are unknown, but studies using an amoebic parasite of reptiles are now yielding information about the molecules and host:parasite interactions in ... | 1997 | 9230696 |
| nitazoxanide in the treatment of cryptosporidial diarrhea and other intestinal parasitic infections associated with acquired immunodeficiency syndrome in tropical africa. | eighteen patients hospitalized with intestinal parasitic infections associated with diarrhea and dehydration completed a study of nitazoxanide in the treatment of cryptosporidium parvum and other intestinal parasitic infections. seventeen of the 18 patients were positive for human immunodeficiency virus. twelve patients were diagnosed with clinical stage 4 acquired immunodeficiency syndrome (aids) according to the 1990 world health organization proposed clinical classification system and cryptos ... | 1997 | 9230795 |
| evaluation of a stage-specific proteolytic enzyme of schistosoma mansoni as a marker of exposure. | cercarial elastase (ce) is one of the first proteins released in the host by invading schistosome cercariae. an enzyme-linked immunosorbent assay (elisa)-formatted immunoassay has been developed to detect antibodies to the stage-specific ce antigen of schistosoma mansoni as marker of exposure. we have evaluated this test system as an epidemiologic tool, using well-characterized sera collected from s. mansoni- and s. haematobium-infected subjects residing in endemic areas and from control subject ... | 1997 | 9230801 |
| a gene homologous to hgl2 of entamoeba histolytica is present and expressed in entamoeba dispar. | 1997 | 9233678 | |
| oral immunization with attenuated vaccine strains of vibrio cholerae expressing a dodecapeptide repeat of the serine-rich entamoeba histolytica protein fused to the cholera toxin b subunit induces systemic and mucosal antiamebic and anti-v. cholerae antibody responses in mice. | entamoeba histolytica is a significant cause of morbidity and mortality worldwide. the serine-rich e. histolytica protein (srehp) is a surface-expressed trophozoite protein that includes multiple hydrophilic tandem repeats. a purified fusion protein between the dodecapeptide repeat of srehp and cholera toxin b subunit (ctb) has previously been shown to be immunogenic in mice after oral inoculation when cholera toxin is coadministered as an immunoadjuvant. we engineered a live attenuated el tor v ... | 1997 | 9234763 |
| transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite entamoeba histolytica. | entamoeba histolytica is a single cell eukaryote that is the etiologic agent of amoebic colitis. core promoter elements of e. histolytica protein encoding genes include a tata-like sequence (gtatttaaag/c) at -30, a novel element designated gaac (gaact) that has a variable location between tata and the site of transcription initiation, and a putative initiator (inr) element (aaaaattca) overlying the site of transcription initiation. the presence of three separate conserved sequences in a eukaryot ... | 1997 | 9238060 |
| genetic transformation of parasitic protozoa. | 1997 | 9241817 | |
| differentiation of entamoeba histolytica and entamoeba dispar from german travelers and residents of endemic areas. | entamoeba histolytica and e. dispar detected in fecal samples of german travelers and residents of tropical and subtropical countries were cultured and differentiated by the polymerase chain reaction (pcr) and hexokinase isoenzyme typing. twenty-one of 60 isolates were identified as e. histolytica. it was shown that 13 of 18 german short-term travelers were infected with e. histolytica, whereas only three of 22 of the examined inhabitants of tropical countries and five of 20 german long-term tra ... | 1997 | 9242322 |
| analysis of expressed sequence tags (ests) of the parasitic protozoa entamoeba histolytica. | a directional cdna library constructed from mrna of the trophozoite of entamoeba histolytica hm-1:imss strain was used for the generation of expressed sequence tags (ests). from 5' ends of the distinct cdna clones, 105 ests were obtained. of these, 30 clones (29%) were previously known e. histolytica genes. forty-five clones (42%) had matches with entries for other organisms in the databases. these new e. histolytica genes constituted a broad range of transcripts distributed among cytoplasmic st ... | 1997 | 9245698 |
| evidence for loss of mitochondria in microsporidia from a mitochondrial-type hsp70 in nosema locustae. | in molecular phylogenies based on ribosomal rna, three amitochondriate protist lineages, microsporidia, metamonada (including diplomonads) and parabasala (including trichomonads), are the earliest offshoots of the eukaryotic tree. as an explantation for the lack of mitochondria in these organisms, the hypothesis that they have diverged before the mitochondrial endosymbiosis is preferred to the less parsimonious hypothesis of several independent losses of the organelle. nevertheless, if they had ... | 1997 | 9247927 |
| sequencing, expression and properties of triosephosphate isomerase from entamoeba histolytica. | we have isolated a cdna clone of the glycolytic enzyme, triosephosphate isomerase (tpi) from entamoeba histolytica. degenerate oligonucleotides obtained by reverse translation of conserved polypeptide sequences, derived from tpis of other organisms, were used to amplify a 450-bp fragment using e. histolytica cdna as a template. the fragment was used to screen a cdna library. the isolated cdna, encoding a protein of 261 amino acids, shares 43-52.6% positional identity with other known protozoan t ... | 1997 | 9249046 |
| cutaneous amebiasis in an iranian immunodeficient alcoholic: immunochemical and histological study. | 1997 | 9252762 | |
| colonic involvement in patients with amebic liver abscess: endoscopic findings. | amebic liver abscess is the most common form of extraintestinal amebiasis. although the parasite enters the liver via the portal vein after invading large bowel mucosa, only 15% to 30% of patients have diarrhea. this study was done to evaluate colonic involvement in patients with amebic liver abscesses. | 1997 | 9260703 |
| role of prostaglandin e2 on amoebic liver abscess formation in hamsters. | entamoeba histolytica can modulate macrophage functions and cytokine production by a prostaglandin e2 (pge2) mechanism. to study the participation of pge2 on amoebic liver abscess formation, we tested the effect of the pg synthesis inhibitor indomethacin (indo) on abscess development in hamsters infected intrahepatically with e. histolytica trophozoites. male infected animals had higher levels of plasma pge2 (5.7 +/- 0.7 pg/ml pre-infection; 26.0 +/- 2.0 pg/ml 7 days postinfection; p < 0.001). i ... | 1997 | 9261861 |
| purification of alcohol dehydrogenase from entamoeba histolytica and saccharomyces cerevisiae using zinc-affinity chromatography. | we have developed a single-step method for the purification of nadp(+)-dependent alcohol dehydrogenase from entamoeba histolytica and nad(+)-dependent alcohol dehydrogenase from saccharomyces cerevisiae. it is based on the affinity for zinc of both enzymes. the amebic enzyme was purified almost 800 times with a recovery of 54% and the yeast enzyme was purified 30 times with a recovery of 100%. the kinetic constants of the purified enzymes were similar to those reported for other purification met ... | 1997 | 9268681 |
| first isolation and characterization in humans of entamoeba histolytica (laboratory-made) zymodeme xx. | isoenzyme analysis by starch-gel electrophoresis has proved to be a useful method for the biochemical differentiation of pathogenic entamoeba histolytica and non-pathogenic e. dispar isolates. of the known 24 zymodemes, 3 are laboratory-made and have not previously been identified in humans. parasitology screening was carried out in a psychiatric institution. two amebic stocks were isolated and characterized that had never previously been found in humans and that have protein patterns identical ... | 1997 | 9272564 |
| a new method for isolation and differentiation of native entamoeba histolytica and e. dispar cysts from fecal samples. | a new method for the purification of protozoan cysts from feces was established, allowing to isolation of native cysts. the procedure consists of two sucrose-density gradients and enzymatic digestion of cellulose particles by cellulase and can be accomplished in a few hours. the cyst fractions were differentiated into entamoeba histolytica and e. dispar using the dna probes p145 and b133 and a dot-blot test. | 1997 | 9272565 |
| entamoeba histolytica contains a gene encoding a homologue to the 54 kda subunit of the signal recognition particle. | we have determined the nucleotide sequence and predicted amino acid sequence of the 54 kda subunit of the signal recognition particle (srp54) from the amitochondrial protist entamoeba histolytica. the srp54 gene was isolated from a genomic library using a polymerase chain reaction (pcr) probe. nucleotide sequence analysis of a 2.3 kb fragment, derived from a 7 kb genomic clone, revealed an open reading frame encoding a protein of 487 amino acids (mw 53.8 kda). the identities of the predicted ami ... | 1997 | 9274882 |
| comparison of use of enzyme-linked immunosorbent assay-based kits and pcr amplification of rrna genes for simultaneous detection of entamoeba histolytica and e. dispar. | a comparison of the use of three commercially available enzyme-linked immunosorbent assay-based kits and pcr amplification of rrna genes to detect and differentiate entamoeba histolytica from e. dispar was carried out. only the techlab kit did not cross-react with e. dispar antigens, but it was 100 times less sensitive than pcr in detection of and differentiation between the two types of entamoeba. | 1997 | 9276425 |
| entamoeba taxonomy. | 1997 | 9277015 | |
| the effect of in vitro bacterial association on virulence of entamoeba histolytica. | when trophozoites of axenic e. histolytica strains nih : 200 were associated in vitro with escherichia coli k12 for three hours at 37 degrees c, the virulence was enhanced as shown by increased cytopathogenicity to baby hamster kidney (bhk) cell monolayer. further, the trophozoites were observed to adhere to the polystyrene surface, a hitherto unreported phenomenon. the co-association of e. histolytica trophozoites with bacteroides fragilis and clostridium perfringens for three hours duration at ... | 1997 | 9277039 |
| thermodynamics of metal ion binding and denaturation of a calcium binding protein from entamoeba histolytica. | the thermodynamics of the binding of calcium and magnesium ions to a calcium binding protein from entamoeba histolytica was investigated by isothermal titration calorimetry (itc) in 20 mm mops buffer (ph 7.0) at 20 degrees c. enthalpy titration curves of calcium show the presence of four ca2+ binding sites. there exist two low-affinity sites for ca2+, both of which are exothermic in nature and with positive cooperative interaction between them. two other high affinity sites for ca2+ exist of whi ... | 1997 | 9283081 |
| necrosis versus apoptosis as the mechanism of target cell death induced by entamoeba histolytica. | the human pathogen entamoeba histolytica is known to kill a variety of host cells, including leukocytes. using human myeloid cells as targets, we studied whether cytotoxicity of amoebic trophozoites in vitro is equivalent to the induction of apoptosis or whether these target cells die via necrosis. based upon morphological criteria, incubation of target cells with amoebae resulted in necrosis, with cell swelling, rupture of plasma membrane, and release of cell contents including nucleic acids be ... | 1997 | 9284127 |
| neutrophils play a critical role in early resistance to amebic liver abscesses in severe combined immunodeficient mice. | animal models of liver abscess formation with entamoeba histolytica suggest that the neutrophil is the first cell of the host immune system to interact with the invading ameba. in vitro studies have suggested that lysis of neutrophils by virulent amebae may exacerbate the damage seen in amebic liver abscesses. to investigate the role of neutrophils in vivo, we used the severe combined immunodeficient (scid) mouse model of amebic liver abscess formation and compared liver damage in neutrophil-dep ... | 1997 | 9284178 |
| does non-dysenteric intestinal amoebiasis exist? | chronic abdominal pain and frequent bowel disturbance are common symptoms experienced by more than 15% of apparently healthy people. in areas endemic for entamoeba histolytica infection, these symptoms are often diagnosed as non-dysenteric intestinal amoebiasis even though no causal relation between such symptoms and e histolytica has been established and clinical presentation of non-dysenteric intestinal amoebiasis and irritable bowel syndrome (ibs) is not distinct. this study was done to asses ... | 1997 | 8996420 |
| identification of entamoeba histolytica and e. dispar cysts in stool by polymerase chain reaction. | an attempt to identify cysts of entamoeba histolytica and e. dispar in human stool was conducted by polymerase chain reaction (pcr) using two sets of primers (p11 plus p12 and p13 plus p14) specific for either species of ameba. the cysts in stool specimens obtained from 12 infected individuals were concentrated, freeze-thawed, and treated with triton x-100 before their examination by pcr. the results of pcr on the cysts were generally consistent with data obtained by pcr on ameba trophozoites ha ... | 1997 | 9000244 |
| waterborne protozoan pathogens. | protozoan parasites were the most frequently identified etiologic agents in waterborne disease outbreak from 1991 to 1994. the waterborne parasites giardia lamblia, naegleria fowleri, acanthamoeba spp., entamoeba histolytica, cryptosporidium parvum, cyclospora cayetanesis, isospora belli, and the microsporidia are reviewed. for each parasite, the review includes history, life cycle, incidence, symptoms, and therapy. clinical detection methods are compared, and emerging technologies are discussed ... | 1997 | 8993859 |
| entamoeba histolytica and entamoeba dispar infection in children in bangladesh. | the prevalence of infection by the invasive parasite entamoeba histolytica and the noninvasive parasite entamoeba dispar was determined in 2000 children in bangladesh. antigen detection identified more cases of e. histolytica-e. dispar infection than did culture or microscopy. microscopic identification of e. histolytica-e. dispar complex infection in stool did not equate with the diagnosis of amebic dysentery because most amebic infections in this population were due to e. dispar: urban childre ... | 1997 | 9041357 |
| characterization of ehcabp, a calcium-binding protein of entamoeba histolytica and its binding proteins. | a novel calcium-binding protein (ehcabp) has been recently identified and characterized from the protozoan parasite entamoeba histolytica. in order to decipher the function of this protein, a few basic properties were investigated and compared with the ubiquitous ca(2+)-signal transducing protein calmodulin (cam). indirect immunofluorescence and immunoprecipitation analyses using specific antibodies against ehcabp suggest that it is a soluble cytoplasmic protein with no major post-translational ... | 1997 | 9041522 |
| tetracycline-controlled gene expression in entamoeba histolytica. | to provide further tools for functional genetics of the protozoan parasite entamoeba histolytica, we have tested the suitability of the bacterial tn10-encoded tet-repressor/tet-operator system for gene regulation in ameba trophozoites. expression of the tet-repressor within the ameba was driven by the wild-type endogenous lectin gene promoter from episomal transfected plasmids. tetracycline-inducible expression of a reporter gene driven by a modified tet-operator-bearing lectin gene promotor was ... | 1997 | 9041523 |
| a tetracycline-inducible gene expression system in entamoeba histolytica. | we have developed an episomal inducible gene expression system in entamoeba histolytica based on the tetr repressor. the tetr gene was placed under control of 5' and 3' ferredoxin (fdx) regulatory sequences on a plasmid encoding the hygromycin resistance gene directed by 5' and 3' hgl sequences. the reporter luciferase constructs were introduced on a second episome bearing the neomycin resistance gene controlled by 5' and 3' actin sequences. the reporter constructs were driven by the hgl5 promot ... | 1997 | 9041524 |
| the entamoeba histolytica proteasome alpha-subunit gene. | 1997 | 9041528 | |
| protozoal agents: what are the dangers for the public water supply? | safe and efficient treatment of drinking water has been one of the major public health advances of the twentieth century. people in developed countries generally take for granted that their water is safe to drink, a luxury the majority of the world's population does not have. the leading cause of infant mortality in the developing world is infectious diarrhea, and the prevalence of diarrheal pathogens is largely influenced by the quality and quantity of clean water available for drinking and was ... | 1997 | 9046966 |
| antimicrobial peptides of leukocytes. | leukocytes use an array of antimicrobial peptides and proteins to help them destroy invading microorganisms. these endogenous antibiotic molecules are remarkable for their structural variety, rapid evolutionary divergence, intraspecies variation, and complex yet subtle interactions with their targets. this arsenal has been studied most extensively in neutrophils, where its members include lactoferrin, secretory phospholipase a2, lysozyme, and the cathelicidins in the secretory granule compartmen ... | 1997 | 9050380 |
| virulent and avirulent entamoeba histolytica and e. dispar differ in their cell surface phosphorylated glycolipids. | virulent strains of entamoeba histolytica have been reported to produce a mixture of phosphoglycoconjugates that share some structural features with the lipophosphoglycans (lpgs) of leishmania. purification of these glycoconjugates is essential to their precise structural characterization. in this study we have extracted 'lpg-like' molecules from various virulent e. histolytica strains and purified on the basis of charge differences, 2 apparently related glycoconjugates a 'lpg' and a 'lipophosph ... | 1997 | 9051918 |
| intragastric immunization of rats with entamoeba histolytica trophozoites induces cecal mucosal ige, eosinophilic infiltration, and type i hypersensitivity. | we have investigated the role of ige in the local immunity of intestinal amebiasis, a parasitic infection known to induce specific antibody-forming cells (afc) and iga antibodies in rodents and humans. we found that intragastric immunization of rats with glutaraldehyde-fixed entamoeba histolytica trophozoites significantly increased antiameba afc in the peyer's patches and spleen and that the lamina propria of the cecum from immunized animals was infiltrated by eosinophils armed with ige antibod ... | 1997 | 9073545 |
| cytoplasmic dna in entamoeba histolytica: its biological significance. | we report a study on the dna organization in entamoeba histolytica using a ribosomal dna probe. the rdna genes were found forming mers which were separated in a typical ladder pattern by pulse field electrophoresis. dna rosette structures were visualized through electron microscopy in dna eluted from bands recognized by the ribosomal probe. the in situ hybridization experiments using a dna probe suggested that the rdna genes are portioned between the nucleus and a cytoplasmic structure. these fi ... | 1997 | 9078580 |
| acute diarrhea in under five-year-old children admitted to king mongkut prachomklao hospital, phetchaburi province. | a prospective epidemiological and clinical study of acute diarrhea in children under 5 years old was done at king mongkut prachomklao hospital in order to provide baseline data for health officers to make a strategic plan to reduce the diarrheal mortality and morbidity, which is one of the mid-decade goals for children. there were 105 cases of acute diarrhea patients admitted to the pediatric ward between may 1995 and april 1996. seventy-six per cent of them were in the younger age group (> 1 mo ... | 1997 | 9078814 |
| molecular modeling of amoebapore and nk-lysin: a four-alpha-helix bundle motif of cytolytic peptides from distantly related organisms. | amoebapore of the protozoan entamoeba histolytica and nk-lysin of porcine cytotoxic lymphocytes are effector peptides from organisms separated extremely early in their evolutionary paths. the peptides intrigued us, however, with indications of some functional similarity. we thus wanted to derive and compare predictions for their as yet unknown three-dimensional structures as a guide for and to be tested by further experiments. | 1997 | 9080198 |
| molecular characterization of the entamoeba histolytica enolase gene and modelling of the predicted protein. | entamoeba histolytica obtains its energy mainly from glucose fermentation. enzymes involved in this pathway could be potential targets for antiparasite drugs. here we report the molecular characterization of the e. histolytica enolase gene (ehenl-i), which in a single copy is located on the 1.6 mb chromosome. it is transcribed into a 1.4 kb mrna which starts 13 nucleotides upstream of the atg start codon. the sequence tataag, at -31, interacted with nuclear proteins suggesting that it has a tata ... | 1997 | 9084139 |
| the root hair defective3 gene encodes an evolutionarily conserved protein with gtp-binding motifs and is required for regulated cell enlargement in arabidopsis. | in plants, morphogenesis is largely determined by the orientation and extent of cell enlargement. to define the molecular mechanisms regulating plant cell enlargement, we have conducted a molecular genetic analysis of the root hair defective3 (rhd3) gene of arabidopsis thaliana. mutations affecting the rhd3 gene were found to alter cell size, but not cell number, in tissues throughout the plant. genetic and physiological analyses suggest that the rhd3 gene is not required for proper cell type sp ... | 1997 | 9087433 |
| a mechanism of acquired resistance to complement-mediated lysis by entamoeba histolytica. | some entamoeba histolytica strains resist complement-mediated lysis by serum. susceptible and resistant strains activate the complement system equivalently, but resistant amebas evade killing by membrane attack complexes. our objective was to determine the mechanism by which trophozoites of e. histolytica resist lysis by human serum. amebas were made resistant to lysis by incubation with increasing concentrations of normal human serum. the possibility that resistant cells ingest membrane attack ... | 1997 | 9105303 |
| cloning and expression of chitinases of entamoebae. | entamoeba histolytica (eh) and entamoeba dispar (ed) are protozoan parasites that infect hundreds of millions of persons. in the colonic lumen, amebae form chitin-walled cysts, the infectious stage of the parasite. entamoeba invadens (ei), which infects reptiles and is a model for amebic encystation, produces chitin synthase and chitinase during encystation. ei cysts formation is blocked by the chitinase-inhibitor allosamidin. here molecular cloning techniques were used to identify homologous ge ... | 1997 | 9106188 |
| electron probe analysis and biochemical characterization of electron-dense granules secreted by entamoeba histolytica. | the interaction of entamoeba histolytica with collagen induces the intracellular formation and release of electron-dense granules (edgs) containing collagenase activity which are important in the pathogenicity of this parasite. purified edgs contain at least 25 polypeptides with acidic pis, nine gelatinase activities, small molecules, including inorganic phosphate (pi), pyrophosphate (pp) and other elements, including na, mg, s, cl, k, ca and fe as measured by scanning transmission electron micr ... | 1997 | 9106196 |
| an improved colorimetric pcr-based method for detection and differentiation of entamoeba histolytica and entamoeba dispar in feces. | the epidemiological implications of the recent separation of "entamoeba histolytica" into two separate species, pathogenic e. histolytica sensu stricto and commensal e. dispar, will not become apparent without methods of distinguishing between them which are applicable to large numbers of specimens. we have modified a pcr-based method to produce such a technique which may be completed in 1 day while still identifying 10(-1) e. histolytica and 1 to 10 e. dispar trophozoites per g of feces when pr ... | 1997 | 9114390 |
| human intestinal epithelial cells produce proinflammatory cytokines in response to infection in a scid mouse-human intestinal xenograft model of amebiasis. | the protozoan parasite entamoeba histolytica causes amebic dysentery and amebic liver abscess, diseases associated with significant morbidity and mortality worldwide. e. histolytica infection appears to involve the initial attachment of amebic trophozoites to intestinal epithelial cells, followed by lysis of these cells and subsequent invasion into the submucosa. a recent in vitro study (l. eckmann, s. l. reed, j. r. smith, and m. f. kagnoff, j. clin. invest. 96:1269-1279, 1995) demonstrated tha ... | 1997 | 9125540 |
| interleukin (il)-2, il-4, and tumor necrosis factor-alpha responses during entamoeba histolytica liver abscess development in gerbils. | to determine cytokine production patterns during hepatic amebiasis, gerbils were infected with entamoeba histolytica in the liver. then spleen and hepatic lymph node cell proliferation and interleukin (il)-2 (a th1 marker), il-4 (a th2 marker), and tumor necrosis factor-alpha (tnf-alpha) production in response to concanavalin a and amebic antigen in vitro were quantified. early abscess development (day 5 after inoculation) coincided with il-2, il-4, and low tnf-alpha production and strong lympho ... | 1997 | 9129082 |
| characterization of nucleoside uptake and transport in entamoeba histolytica. | the uptake and transportation of purine and pyrimidine based nucleosides by trophozoites of axenically grown entamoeba histolytica (hmi-imss) were studied. the trophozoites transported adenosine and its analog tubercidin (1 microm) at a significant rate but poor transportation was observed in case of uridine (about 10% relative rate), inosine (3%), thymidine (2%) and formycin b (1%). the km for adenosine was 160 +/- 42 microm. unlabeled nucleosides (100 microm) inhibited adenosine and tubercidin ... | 1997 | 9134559 |
| in vitro entamoeba histolytica adhesion to human endothelium: a comparison using two strains of different virulence. | extraintestinal dissemination of entamoeba histolytica is frequently manifested by the life-threatening amebic liver abscess (ala). the hepatic establishment of amebas implies invasion of blood vessels and contact with the endothelium. by means of a fluorescence-based quantitative adhesion assay, we assessed the binding to human endothelial cells of two e. histolytica strains of different virulence. the highly virulent strain (l-a) adhered substantially more strongly to unstimulated endothelium ... | 1997 | 9134567 |
| entamoeba histolytica stimulates interleukin 8 from human colonic epithelial cells without parasite-enterocyte contact. | the mechanisms involved in the initiation of host mucosal inflammation in amebiasis are not fully understood. this study characterized the effect of entamoeba histolytica components on interleukin 8 (il-8) gene expression in human colonic cells. | 1997 | 9136832 |
| a novel cytoplasmic structure containing dna networks in entamoeba histolytica trophozoites. | we report here the presence of cytoplasmic dna arranged in networks in the trophozoites of the human parasite entamoeba histolytica. cytoplasmic dna was detected in live trophozoites in a structure that we called ehko, using the fluorescent dye acridine orange, and by in situ hybridization to trophozoites with a rdna probe. the ehko was found in the axenically grown clones a, l6 (strain hmi:imss) and mavax (strain mav) and in the polyxenically grown clone mavpx (strain mav). bacteria present in ... | 1997 | 9150258 |
| identification of an epitope on the entamoeba histolytica 170-kd lectin conferring antibody-mediated protection against invasive amebiasis. | the emergence of multidrug-resistant organisms and the failure to eradicate infection by a number of important pathogens has led to increased efforts to develop vaccines to prevent infectious diseases. however, the nature of the immune response to vaccination with a given antigen can be complex and unpredictable. an example is the galactose- and n-acetylgalactosamine-inhibitable lectin, a surface antigen of entamoeba histolytica that has been identified as a major candidate in a vaccine to preve ... | 1997 | 9151705 |
| evaluation of a rapid enzyme immunoassay for diagnosis of hepatic amoebiasis. | we compared the capability of rapid enzyme immunoassay (eia) to detect antiamoebic antibodies during hepatic amoebiasis with those of indirect hemagglutination and latex agglutination. eia is simple to perform and rapid (20 min) and does not require any special equipment (optical reading is sufficient). eia of 143 sera (including 43 from patients with proven hepatic amoebic abscess, 33 from patients with other hepatic disorders and/or parasitic infections, and 67 from healthy individuals) yielde ... | 1997 | 9163475 |
| [infectious disease in kenya--epidemiological study of diarrhoeal disease in children]. | i participated in a project concerning diarrhea in children caused by bacteria (japan international cooperation association) in the republic of kenya between april 1993 and march 1994. according to the health information report by the ministry of health in 1993, diarrhea was at the 5th most frequent disease among outpatients and these patients numbered about eight hundred thousand. on microbiological examination, diarrhoegenic agents were detected from 767 of 1,362 (56.3%) fecal specimens collec ... | 1997 | 9170969 |
| evidence for the bacterial origin of genes encoding fermentation enzymes of the amitochondriate protozoan parasite entamoeba histolytica. | entamoeba histolytica is an amitochondriate protozoan parasite with numerous bacterium-like fermentation enzymes including the pyruvate:ferredoxin oxidoreductase (por), ferredoxin (fd), and alcohol dehydrogenase e (adhe). the goal of this study was to determine whether the genes encoding these cytosolic e. histolytica fermentation enzymes might derive from a bacterium by horizontal transfer, as has previously been suggested for e. histolytica genes encoding heat shock protein 60, nicotinamide nu ... | 1997 | 9171424 |
| lssp-pcr for characterization of strains of entamoeba histolytica isolated in brazil. | strains of entamoeba histolytica isolated in brazil were characterized using the low-stringency single specific primer pcr (lssp-pcr), that detects single or multiple mutations in gene size dna fragments. using this technique, a 482-bp genomic dna fragment from a structural gene in 8 strains and 2 clones of e. histolytica, isolated from symptomatic and asymptomatic patients in brazil, including pathogenic and non-pathogenic zymodemes were studied. the results obtained indicate that lssp-pcr is a ... | 1997 | 9172422 |
| up-regulation of action mrna and reorganization of the cytoskeleton in entamoeba histolytica trophozoites. | actin mrna levels were measured in entamoeba histolytica trophozoites after experimentally inducing changes in the organization of the cytoskeleton. the treatment of trophozoites with forskolin, n6,2'-o-dibutyryl-adenosine 3':5'-cyclic monophosphate, and phorbol myristate acetate induced the organization of actin into multiple dots and defined structures with a concomitant increase in f-actin content. cytochalasin d elicited polarization of the structured actin and formation of aggregates, as we ... | 1997 | 9172829 |
| presence of very high prevalence and intensity of infection with fasciola hepatica among aymara children from the northern bolivian altiplano. | coprological studies of school children from four communities in the northern bolivian altiplano were carried out in order to estimate the prevalences and intensities of fasciola hepatica infection. single stool specimens were collected at random from 558 school children (308 boys and 250 girls) aged 5-19 years old. nineteen different parasite species (13 protozoan and six helminths) were detected. of the children examined, 98.7% (96.5-100%) presented infection with at least one parasite species ... | 1997 | 9177091 |
| molecular characterization of myosin ib from the lower eukaryote entamoeba histolytica, a human parasite. | the complete amino acid sequence of the entamoeba histolytica unconventional myosin ib (eh-myosin ib) is reported. sequencing of overlapping cdna fragments reveals a single open reading frame which predicts a 130 kda protein of 1049 aa. eh-myosin ib presents the three characteristics domains of myosins i subclass 1. this protein presents homology with myosins ib from other amoebae, but striking homologies with vertebrate unconventional myosins were also observed. the predicted actin and atp-bind ... | 1997 | 9178268 |
| molecular biology of the hexokinase isoenzyme pattern that distinguishes pathogenic entamoeba histolytica from nonpathogenic entamoeba dispar. | the electrophoretic patterns of hexokinase and phosphoglucomutase have been widely used to distinguish entamoeba histolytica from entamoeba dispar isolates. although e. histolytica and e. dispar, previously called pathogenic and nonpathogenic entamoeba histolytica, differ clearly in sequences of many homologous genes, a conversion between the two has been reported by several laboratories, in each case showing the conversion of hexokinase (atp, d-hexose 6-phosphotransferase, ec 2.7.1.1) isoenzyme ... | 1997 | 9178270 |
| protection in a gerbil model of amebiasis by oral immunization with salmonella expressing the galactose/n-acetyl d-galactosamine inhibitable lectin of entamoeba histolytica. | infection with the enteric parasite entamoeba histolytica can result in colitis and dysentery as well as abscesses at extra-intestinal sites. an effective vaccine must be able to protect against both mucosal and systemic disease. in this study an attenuated salmonella strain that expressed a portion of the galnac lectin of e, histolytica was used to orally immunize gerbils. animals were challenged by intrahepatic injection of amebic trophozoites. a significant decrease in size of amebic liver ab ... | 1997 | 9178467 |
| etiology of bloody diarrhea in bolivian children: implications for empiric therapy. bolivian dysentery study group. | in bolivia, few data are available to guide empiric therapy for bloody diarrhea. a study was conducted between december 1994 and april 1995 to identify organisms causing bloody diarrhea in bolivian children. rectal swabs from children <5 years old with bloody diarrhea were examined for salmonella, shigella, and campylobacter organisms; fecal specimens were examined for entamoeba histolytica. a bacterial pathogen was identified in specimens from 55 patients (41%). shigella organisms were found in ... | 1997 | 9180200 |
| enteropathogens associated with diarrhea among military personnel during operation bright star 96, in alexandria, egypt. | this study investigated the microbial causes of diarrheal disease among u.s. troops deployed near alexandria, egypt, during october 1995. bacterial causes associated with 19 cases of diarrhea included: enterotoxigenic escherichia coli (etec), 42% (21% heat-stable, 11% heat-labile, and 11% heat-stable/ heat-labile producers); enteropathogenic e. coli (5.3%); and enteroadherent e. coli (42%). four cases of diarrhea were associated with enteroaggregative e. coli based on probe analysis for enteroag ... | 1997 | 9183160 |
| a 964 bp repetitive dna in entamoeba histolytica is associated with linear "chromosomal" dnas of variable sizes. | 1997 | 9032991 | |
| identification of putative linear and circular dna molecules in the entamoeba histolytica molecular karyotype. | 1997 | 9032992 | |
| organization of entamoeba histolytica ribosomal and structural genes in pfge. | 1997 | 9032993 | |
| in situ location of the ehredox and 16s rdna genes in entamoeba histolytica trophozoites. | 1997 | 9032994 | |
| molecular cloning of histone h1 in entamoeba histolytica and histone h2b in entamoeba histolytica and entamoeba dispar. | 1997 | 9032995 | |
| detection of a human p53-like protein in e. histolytica. | 1997 | 9032996 | |
| the sequence of a highly homologous gene to hgl2* of entamoeba histolytica that is present and expressed in e.dispar. | 1997 | 9032997 | |
| localization of a retinoblastoma-like protein in entamoeba histolytica. | 1997 | 9032998 | |
| a model for unscheduled dna replication in entamoeba histolytica trophozoites. | 1997 | 9032999 | |
| phenotype variability and genetic polymorphism in entamoeba histolytica clonal populations. | 1997 | 9033000 | |
| possible role of transcribed intergenic sequences in entamoeba histolytica. | 1997 | 9033001 | |
| inducible gene expression in entamoeba histolytica mediated by a tetracycline responsive prokaryotic repressor. | 1997 | 9033002 | |
| establishment of an inducible expression system in entamoeba histolytica. | 1997 | 9033003 | |
| upstream regions of rp l21 genes play a role in regulation of expression at the post-transcriptional level in e. histolytica and e. dispar. | 1997 | 9033004 | |
| control of gene expression in entamoeba histolytica by a cis-acting upstream regulatory element. | 1997 | 9033005 | |
| three conserved cis-acting sequences in the core promoter control gene expression in the protozoan parasite entamoeba histolytica. | 1997 | 9033006 | |
| comparison of the entamoeba histolytica tata-binding protein (tbp) structure with other tbp. | 1997 | 9033007 | |
| structural characterization of the entamoeba histolytica enolase gene promoter. | 1997 | 9033008 | |
| stable transfection of entamoeba histolytica trophozoites by lipofection. | 1997 | 9033009 | |
| the secretory pathway of entamoeba histolytica: characterization and expression of the srp54 gene. | 1997 | 9033011 | |
| the secretory pathway of entamoeba histolytica: characterization and expression of the erd2 gene. | 1997 | 9033012 |