Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| identification of emesis-causing bacillus cereus strains by polymerase chain reaction: preliminary results. | 2007 | 17682912 | |
| inhibitory effect of melastoma candidum d. don acetone extract on foodborne pathogenic bacteria survival in food products. | melastoma candidum d. don, a taiwanese folk medicinal plant, has high levels of antibacterial and bactericidal activity. our aim was to determine whether and to what extent an acetone extract of this plant inhibits the growth of foodborne pathogenic bacteria. m. candidum acetone extract had marked inhibitory effect on test bacteria introduced into sliced pork, which was then stored at 4 degrees c. at the end of storage (day 12), the bacterial concentrations dropped by 1.59 to 2.91 log cfu/g comp ... | 2007 | 17685331 |
| site-specific and asymmetric hydrolysis of prochiral 2-phenyl-1,3-propanediol diacetate by a bacterial esterase from an isolated strain. | bacillus cereus 809a and burkholderia sp. 711c were isolated from soil. these strains demonstrate hydrolysis activity towards prochiral 2-phenyl-1,3-propanediol diacetate and accumulated the corresponding chiral monoacetates into the reaction mixture. when 2-phenyl 1,3-propanediol diacetate was used as a substrate, the produced monoacetates with burkholderia sp. 711c were obtained in a racemic form but that produced by bacillus cereus 809a showed an excess of the (s)-form. the resting cell react ... | 2007 | 17690472 |
| carbohydrazones of substituted salicylaldehydes as potential lead compounds for the development of narrow-spectrum antimicrobials. | certain substituted salicylaldehydes are known to have highly potent antimicrobial activity against bacteria and fungi, but the mechanism underlying this remarkable activity is not known, and almost nothing has been reported on the effects of further modification of the structures, such as the formation of hydrazone-type derivatives. we report now a study on the antimicrobial properties of the carbohydrazone derivatives of several substituted salicylaldehydes. the compounds studied were synthesi ... | 2007 | 17913061 |
| antimicrobial properties of substituted salicylaldehydes and related compounds. | a systematic survey of the antimicrobial properties of substituted salicylaldehydes and some related aromatic aldehydes is reported. a total of 23 different compounds, each at four different concentrations, were studied using a panel of seven microbes (aspergillus niger, bacillus cereus, candida albicans, escherichia coli, pseudomonas aeruginosa, saccharomyces cerevisiae and staphylococcus epidermidis) and employing the paper disc agar diffusion method. several aldehydes, most notably halogenate ... | 2007 | 17913062 |
| antimicrobial activity of two antitumour agents and ribonucleotide reductase inhibitors, pyridine-2-carboxaldehyde thiosemicarbazone and the acetate form of its copper(ii) chelate. | some copper chelates have potent antitumour activity, and in some cases also the free ligands have activity in vivo. yet, little is known about their antimicrobial properties. copper(ii) chelates of the thiosemicarbazones of a-n-heterocyclic carboxaldehydes constitute one important group of such agents, also their ligands having marked antitumour activity. both the ligands and chelates inhibit ribonucleotide reductase. some ligands have been or are under clinical trials as antineoplastic agents. ... | 2007 | 17913063 |
| antimicrobial activity of extracts of the lichen parmelia sulcata and its salazinic acid constituent. | the antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. all of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, yersinia enterocolitica, staphylococcus aureus ... | 2007 | 17913083 |
| the zn2 position in metallo-beta-lactamases is critical for activity: a study on chimeric metal sites on a conserved protein scaffold. | metallo-beta-lactamases (mbetals) are bacterial zn(ii)-dependent hydrolases that confer broad-spectrum resistance to beta-lactam antibiotics. these enzymes can be subdivided into three subclasses (b1, b2 and b3) that differ in their metal binding sites and their characteristic tertiary structure. to date there are no clinically useful pan-mbetal inhibitors available, mainly due to the unawareness of key catalytic features common to all mbetal brands. here we have designed, expressed and characte ... | 2007 | 17915249 |
| surface reaction of bacillus cereus biomass and its biosorption for lead and copper ions. | in this study, the surface chemical functional groups of bacillus cereus biomass were identified by fourier transform infrared (ftir) analytical technique. it had been shown that the b. cereus cells mainly contained carboxyl, hydroxyl, phosphate, amino and amide functional groups. the potentiometric titration was conducted to explain the surface acid-base properties of aqueous b. cereus biomass. the computer program fiteql 4.0 was used to perform the model calculations. the optimization results ... | 2007 | 17915701 |
| effect of natural preservatives on the growth of histamine producing bacteria. | present study deals with the hampering of the growth of histamine producing bacteria (hpb), by using nacl and spices which are easily available and cheaper cost wise. for this experiment, four strains of hpb viz. vibrio parahaemolyticus, bacillus cereus, pseudomonas aeruginosa and proteus mirabilis were tested against 1 to 10% concentrations of nacl and 1 to 5% concentrations of natural preservatives (turmeric, ginger and garlic) in a basal medium. hpb showed different growth rates at different ... | 2007 | 17915763 |
| biosurfactant production and surface translocation are regulated by plcr in bacillus cereus atcc 14579 under low-nutrient conditions. | bacillus cereus atcc 14579 can respond to nutrient changes by adopting different forms of surface translocation. the b. cereus atcc 14579 deltaplcr mutant, but not the wild type, formed dendritic (branched) patterns on eps [a low-nutrient medium that contains 7.0 g k(2)hpo(4), 3.0 g kh(2)po(4), 0.1 g mgso(4).7h(2)o, 0.1 g (nh(4))(2)so(4), 0.01 g cacl(2), 0.001 g feso(4), 0.1 g nacl, 1.0 g glucose, and 125 mg yeast extract per liter] containing 0.7% agar. the dendritic patterns formed by sliding ... | 2007 | 17921286 |
| differential analysis of bacillus anthracis after px01 plasmid curing and comprehensive data on bacillus anthracis infection in macrophages and glial cells. | bacillus anthracis is a gram-positive bacterial organism responsible for anthrax. this organism has two pathogenic plasmids: px01 and px02. the genetic function of px01, which comprises about 198 kb, is not known, except for a region called the pathogenic island, which contains three genes-pag, lef, and cya-that code for three toxic proteins. a 2-d difference gel electrophoresis (2-d dige) system was used to verify the existence of proteins controlled by the px01 plasmid, and protein regulation ... | 2007 | 17880004 |
| development of flexible antimicrobial films using essential oils as active agents. | the antimicrobial activity in the vapor-phase of laboratory-made flexible films of polypropylene (pp) and polyethylene/ethylene vinyl alcohol copolymer (pe/evoh) incorporating essential oil of cinnamon ( cinnamomum zeylanicum), oregano ( origanum vulgare), clove ( syzygium aromaticum), or cinnamon fortified with cinnamaldehyde was evaluated against a wide range of microorganisms: the gram-negative bacteria escherichia coli, yersinia enterocolitica, pseudomonas aeruginosa, and salmonella cholerae ... | 2007 | 17880148 |
| enhanced secondary metabolite (tanshinone) production of salvia miltiorrhiza hairy roots in a novel root-bacteria coculture process. | salvia miltiorrhiza bunge (lamiaceae) hairy root cultures were inoculated (at 0.02 and 0.2% v/v) and co-cultured with bacillus cereus bacteria. the root biomass growth was inhibited significantly by the bacteria inoculated to the root culture on the first day (day 0) but not by the bacteria inoculated on days 14 or 21 (in a 28-day overall period). on the other hand, the growth of the bacteria in the hairy root culture was also strongly inhibited by the hairy roots, partially because of the antib ... | 2007 | 17882415 |
| influence of an oil soluble inhibitor on microbiologically influenced corrosion in a diesel transporting pipeline. | microbial degradation of the oil soluble corrosion inhibitor (osci) baker nc 351 contributed to a decrease in inhibitor efficiency. corrosion inhibition efficiency was studied by the rotating cage and flow loop methods. the nature of the biodegradation of the corrosion inhibitor was also analysed using fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry. the influence of bacterial activity on the degradation of the corrosion i ... | 2007 | 17882627 |
| [influence of bacillus cereus on microbiocenosis of gastrointestinal tract in rats]. | the modifying effect of bacillus cereus on intestinal microbiocenosis was investigated in eubiotic and disbiotic female rats. qualitative and quantitative characteristics of gut and mucosal microflora from different parts of rats' intestine were studied before and after intragastral application of b. cereus suspension. the single application of b. cereus suspension resulted in appearance of this bacterium in feces and in parietal mucin from all parts of the intestine. in eubiotic rats compared w ... | 2007 | 17882837 |
| microbial population present in fermented beverage 'cauim' produced by brazilian amerindians. | the tapirapé amerindians of the tapi'itãwa tribe produce several fermented foods and beverages among them the beverage called 'cauim'. this beverage is the main staple food for infants until two years old and their parents. for producing the beverage, several substrates are used, such as: cassava, rice, corn, maize and peanuts. the fermentation using mainly cassava was accomplished and samples were collected for chemical and microbiological analysis. a progressive acidification during the fermen ... | 2007 | 17888538 |
| enzymatic reduction of azo and indigoid compounds. | a customer- and environment-friendly method for the decolorization azo dyes was developed. azoreductases could be used both to bleach hair dyed with azo dyes and to reduce dyes in vat dyeing of textiles. a new reduced nicotinamide adenine dinucleotide-dependent azoreductase of bacillus cereus, which showed high potential for reduction of these dyes, was purified using a combination of ammonium sulfate precipitation and chromatography and had a molecular mass of 21.5 kda. the optimum ph of the az ... | 2007 | 17891390 |
| the exosporium of b. cereus contains a binding site for gc1qr/p33: implication in spore attachment and/or entry. | b. cereus, is a member of a genus of aerobic, gram-positive, spore-forming rod-like bacilli, which includes the deadly, b. anthracis. preliminary experiments have shown that gc1qr binds to b. cereus spores that have been attached to microtiter plates. the present studies were therefore undertaken, to examine if cell surface gc1qr plays a role in b. cereus spore attachment and/or entry. monolayers of human colon carcinoma (caco-2) and lung cells were grown to confluency on 6 mm coverslips in shel ... | 2007 | 17892212 |
| assembly of pili on the surface of bacillus cereus vegetative cells. | vegetative forms of bacillus cereus are reported to form pili, thin protein filaments that protrude up to 1 mum from the bacterial surface. pili are assembled from two precursor proteins, bcpa and bcpb, in a manner requiring a pilus-associated sortase enzyme (srtd). pili are also formed on the surface of bacillus anthracis expressing bcpa-srtd-bcpb. bcpa is distributed throughout the entire pilus, whereas bcpb appears positioned at its tip. in agreement with the hypothesis for pilus assembly in ... | 2007 | 17897374 |
| molecular and biochemical detection of fengycin- and bacillomycin d-producing bacillus spp., antagonistic to fungal pathogens of canola and wheat. | bacillus species are well known for their ability to control plant diseases through various mechanisms, including the production of secondary metabolites. bacillus subtilis dfh08, an antagonist of fusarium graminearum, and other bacillus spp. that are antagonists of common fungal pathogens of canola were screened for peptide synthetase biosynthetic genes of fengycin and bacillomycin d. specific polymerase chain reaction (pcr) primers identified b. subtilis strains dfh08 and 49 for the presence o ... | 2007 | 17898845 |
| molecular characterization of antimicrobial compound produced by lactobacillus acidophilus aa11. | approximately 63 strains of lactobacillus acidophilus were isolated from egyptian home-made cheese and examined for production of antagonism. only eight strains demonstrated inhibitory activity against spoilage microorganisms (i.e. staphylococcus aureus and bacillus cereus) and pathogens (i.e. e. coli, salmonella sp. and shigella sp.). lactobacillus acidophilus aa11 produced higher antimicrobial activity with a wide range of inhibition. the agent aa11 was sensitive to proteolytic enzymes and ret ... | 2007 | 17899791 |
| effectiveness of trisodium phosphate, acidified sodium chlorite, citric acid, and peroxyacids against pathogenic bacteria on poultry during refrigerated storage. | the effects of dipping treatments (15 min) in potable water or in solutions (wt/vol) of 12% trisodium phosphate (tsp), 1,200 ppm acidified sodium chlorite (asc), 2% citric acid (ca), and 220 ppm peroxyacids (pa) on inoculated pathogenic bacteria (listeria monocytogenes, staphylococcus aureus, bacillus cereus, salmonella enteritidis, escherichia coli, and yersinia enterocolitica) and skin ph were investigated throughout storage of chicken legs (days 0, 1, 3, and 5) at 3 +/- 1 degrees c. all chemi ... | 2007 | 17900083 |
| influence of the probiotic bacillus cereus var. toyoi on the intestinal immunity of piglets. | in a feeding trial, sows and piglets were fed with the probiotic bacterium bacillus cereus var. toyoi as a feed additive, and the effects on immune cell populations were examined. the development of the gut immune system was determined for piglets at the ages of 14, 28, 35 and 56 days post partum. tissue samples of the jejunum and the continuous peyer's patch were used for enumeration of intraepithelial lymphocyte populations by fluorescence activated flow cytometry and fluorescence microscopy. ... | 2007 | 17870185 |
| food-poisoning and commercial air travel. | with the introduction of budget airlines and greater competitiveness amongst all airlines, air travel has now become an extremely popular form of travel, presenting its own unique set of risks from food poisoning. foodborne illness associated with air travel is quite uncommon in the modern era. however, when it occurs, it may have serious implications for passengers and when crew are affected, has the potential to threaten safety. quality, safe, in-flight catering relies on high standards of foo ... | 2007 | 17870632 |
| structure and biological activities of beta toxin from staphylococcus aureus. | beta toxin is a neutral sphingomyelinase secreted by certain strains of staphylococcus aureus. this virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. the structure of beta toxin was determined at 2.4-a resolution using crystals that were merohedrally twinned. this structure is similar to that of the sphingomyelinases of listeria ivanovii and bacillus cereus. beta toxin belongs to the dnase i folding superfamily; in addition to sphingomyel ... | 2007 | 17873030 |
| phenolic compounds and antimicrobial activity of olive (olea europaea l. cv. cobrançosa) leaves. | we report the determination of phenolic compounds in olive leaves by reversed-phase hplc/dad, and the evaluation of their in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely gram positive (bacillus cereus, b. subtilis and staphylococcus aureus), gram negative bacteria (pseudomonas aeruginosa, escherichia coli and klebsiella pneumoniae) and fungi (candida albicans and cryptococcus neoformans). seven phenolic compo ... | 2007 | 17873849 |
| incidence and characterization of diarrheal enterotoxins of fecal bacillus cereus isolates associated with diarrhea. | a total of 490 stool specimens were collected from patients with diarrhea and healthy controls without diarrhea to investigate the incidence of bacillus cereus and its enterotoxins. b. cereus was found more significant in stools of persons with diarrhea than without diarrhea (9.5% versus 1.8%, p < 0.05), and was also detected more frequent but not significant in individuals aged > or =1 year and in adults than in children aged <1 year (11% and 8% versus 7.8%, p > 0.05). the hemolytic enterotoxin ... | 2007 | 17878069 |
| impact of the probiotic bacteria enterococcus faecium ncimb 10415 (sf68) and bacillus cereus var. toyoi ncimb 40112 on the development of serum igg and faecal iga of sows and their piglets. | to examine the influence of two different probiotic bacteria on the humoral immune system of swine, two animal studies were carried out with sows and their litters. the sows' feed was supplemented with either enterococcusfaecium ncimb 10415 (sf68) or bacillus cereus var. toyoi ncimb 40112 beginning early in pregnancy. the total iga content in the faeces as well as the total igg concentration in the blood of the sows was recorded before and after weaning. the same parameters were determined in th ... | 2007 | 17760301 |
| bacillus acidiceler sp. nov., isolated from a forensic specimen, containing bacillus anthracis px02 genes. | research at the center for biological defense identified plasmid-borne forms of bacillus anthracis pxo2 genes in a gram-positive, endospore-forming rod, isolated from a forensic specimen considered a credible threat of harbouring anthrax. conventional, commercial and molecular-based methods indicated that the isolate (cbd 119(t)) was not b. anthracis and considered not to be a member of the bacillus cereus group. based on the 16s rrna gene sequence similarities, strain cbd 119(t) was most closel ... | 2007 | 17766868 |
| a systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food. | to determine the diagnostic accuracy of tests for the rapid diagnosis of bacterial food poisoning in clinical and public health practice and to estimate the cost-effectiveness of these assays in a hypothetical population in order to inform policy on the use of these tests. | 2007 | 17803865 |
| maintenance of sterility in 1-ml polypropylene syringes. | the sterility of syringes filled with a growth-promoting broth when stored under various temperature conditions was studied. | 2007 | 17823109 |
| ethnobotanical survey and antibacterial activity of some plants used in guinean traditional medicine. | a total of 418 healers have been interviewed in guinea, a coastal country of west africa, ranging between 7 degrees 30 and 12 degrees 30 of northern latitude and 8 degrees and 15 degrees of western longitude. plant species used by the local inhabitants to treat infectious diseases were identified using ethnobotanical, ethnographic and taxonomic methods. during these investigations, 218 plants were registered, of which the following were the most frequently used: erythrina senegalensis, bridelia ... | 2007 | 17825510 |
| synthesis, spectroscopic and biological aspects of iron(ii) complexes. | five novel coordinated complexes of iron(ii) with ciprofloxacin and neutral bidentate ligands have been prepared and characterized using elemental analyses, magnetic measurements, ir spectra, uv-vis spectral, thermogravimetric analyses, 1h-nmr and 13c-nmr. the antimicrobial activity of the individual ligands, metal salt and metal complexes with respect to bacillus subtilis, escherichia coli, bacillus cereus, staphylococcus aureus, salmonella typhi, serratia marcescens, aspergillus niger, aspergi ... | 2007 | 17847716 |
| synthesis and antimicrobial activity of some new 1,3,4-thiadiazole and 1,2,4-triazole compounds having a d,l-methionine moiety. | new 1,3,4-thiadiazole, 5a-e, and 1,2,4-triazolecompounds 6a-c, containing a d,l-methionine moiety were synthesized by intramolecular cyclization of 1,4-disubstituted thiosemicarbazides 4a-e in acid and alkaline media, respectively. the potential antimicrobial effects of the synthesized compounds were investigated using the staphylococcus aureus atcc 25923, bacillus antracis atcc 8705, bacillus cereus atcc 10987, sarcina lutea atcc 9341 and escherichia coli atcc 25922 strains. the newly synthesiz ... | 2007 | 17693957 |
| [usefulness of the thoracoscopic surgery under local anesthesia and irrigation for the patient with bacillus cereus empyema; report of a case]. | the case was 54-year-old male with some risks such as chronic heart failure, atrial fibrillation, and liver chirrhosis. he was admitted because of severe back pain and diagnosed as empyema by preoperative thoracentesis. by thoracoscopic procedures under local anesthesia, fibrinopurulent tissues were cleaned as much as possible and 3 of chest tubes were replaced. the final diagnosis was bacillus cereus pyothorax by bacterial cultures of pleural effusion. intrathoracic cavity was cleaned with phys ... | 2007 | 17703630 |
| control of microorganisms and reduction of biogenic amines in chicken breast and thigh by irradiation and organic acids. | the effect of irradiation or organic acid treatment of raw chicken breast and thigh meat to control inoculated microorganisms and the production of biogenic amines (ba) was studied. bacillus cereus, enterobacter cloacae, and alcaligenes faecalis were selected and inoculated into raw ground chicken breast and thigh meat at approximately 10(7) cfu/g. the samples were irradiated at 0, 0.5, 1, and 2 kgy or mixed with a 0.2 m solution of acetic, citric, or lactic acid (1 ml for 10 g of meat sample) f ... | 2007 | 17704395 |
| characterization of the adenine nucleoside specific phosphorylase of bacillus cereus. | adenosine phosphorylase, a purine nucleoside phosphorylase endowed with high specificity for adenine nucleosides, was purified 117-fold from vegetative forms of bacillus cereus. the purification procedure included ammonium sulphate fractionation, ph 4 treatment, ion exchange chromatography on deae-sephacel, gel filtration on sephacryl s-300 hr and affinity chromatography on n(6)-adenosyl agarose. the enzyme shows a good stability to both temperature and ph. it appears to be a homohexamer of 164+ ... | 2007 | 17707115 |
| evidence for a dinuclear active site in the metallo-beta-lactamase bcii with substoichiometric co(ii). a new model for metal uptake. | metallo-beta-lactamases are zinc-dependent enzymes that constitute one of the main resistance mechanisms to beta-lactam antibiotics. metallo-beta-lactamases have been characterized both in mono- and dimetallic forms. despite many studies, the role of each metal binding site in substrate binding and catalysis is still unclear. this is mostly due to the difficulties in assessing the metal content and site occupancy in solution. for this reason, co(ii) has been utilized as a useful probe of the act ... | 2007 | 17715135 |
| [open leg fracture with bacillus cereus infection]. | to investigate leg fractures infected with bacillus cereus in a traumatology-orthopedic ward of a university hospital. | 2007 | 17629655 |
| high resolution fesem and tem reveal bacterial spore attachment. | transmission electron microscopy (tem) studies in the 1960s and early 1970s using conventional thin section and freeze fracture methodologies revealed ultrastructural bacterial spore appendages. however, the limited technology at that time necessitated the time-consuming process of imaging serial sections and reconstructing each structure. consequently, the distribution and function of these appendages and their possible role in colonization or pathogenesis remained unknown. by combining high re ... | 2007 | 17637074 |
| walnut (juglans regia l.) leaves: phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. | different cultivars of walnut (juglans regia l.) leaves (cv. lara, franquette, mayette, marbot, mellanaise and parisienne) grown in portugal, were investigated in what concerns phenolic compounds and antimicrobial and antioxidant properties. phenolics analysis was performed by reversed-phase hplc/dad and 10 compounds were identified and quantified: 3- and 5-caffeoylquinic acids, 3- and 4-p-coumaroylquinic acids, p-coumaric acid, quercetin 3-galactoside, quercetin 3-pentoside derivative, querceti ... | 2007 | 17637491 |
| antibacterial effect of five zingiberaceae essential oils. | essential oil obtained by hydrodistillation and two different solvent extractions (petroleum ether and ethanol) from five zingiberaceae species: ginger (zingiber officinale roscoe.), galanga (alpinia galanga sw.), turmeric (curcuma longa l.), kaempferia (boesenbergia pandurata holtt.) and bastard cardamom (amomum xanthioides wall.) was characterized. volatile components of all extracts were analyzed by gas chromatography-mass spectrometry (gc-ms). the major components of ginger, turmeric, galang ... | 2007 | 17960105 |
| systemic dexamethasone and its effect on normal aerobic bacterial flora of cow. | this study was carried out on 17 holestein, heifers, aged between 1 to 2 years for determining the normal aerobic bacterial flora and their changes after dexamethasone injection. swab samples were taken from eye, ear, pharynx and vagina before and 5 days after twice dexamethasone treatment. results indicated that bacillus cereus and corynebacterium pseudotuberculosis had higher frequency of isolations than the other bacterial flora in eye, ear and pharynx. actinomyces pyogenes was isolated with ... | 2007 | 19093453 |
| antibacterial activities of the extracts and conessine from holarrhena floribunda g. don. (apocynaceae). | the methanolic extract and conessine isolated from the stem bark of holarrhena floribunda (hf) were tested for their antibacterial activities on bacillus: bacillus cereus, bacillus subtilis, bacillus megaterium and bacillus stearothermophilus using the disc diffusion method. phytochemical analysis of the crude extract and fractions was also conducted. the inhibition parameters of the crude methanol extract and the total alkaloid fraction were determined using the macrodilution method. the result ... | 2007 | 20161899 |
| microbial load and antimicrobial property of two nigerian herbal remedies. | the use of herbal remedies had increased significantly in the last one decade in nigeria. this has led to the production of herbal products with bogus claims. the microbial quality and antibacterial properties of two nigerian herbal remedies with such claimed efficacy of curing all manners of microbial diseases were assessed. the herbal remedies were discovered to be contaminated with the following microorganisms: bacillus subtilis, bacillus coagulans, bacillus cereus, basidiobotrytis sp, oedoce ... | 2007 | 20162058 |
| antimicrobial activity of various cationic molecules on foodborne pathogens. | antibacterial effects of various arginine- and lysine-rich polycationic proteins and polymers were evaluated by broth and solid dilution assay on a range of foodborne pathogens, gram-positive and gram-negative bacteria. the minimum inhibitory concentration (mic) and the minimum bactericidal concentration (mbc) of α-poly-l-lysine (poly-lys), α-poly-l-arginine (poly-arg) and protamines from herring sperm (clupeine sulphate) and salmon sperm (salmine sulphate) were determined on bacillus subtilis, ... | 2007 | 27517822 |
| an outbreak of foodborne diarrheal illness among soldiers in mina during hajj: the role of consumer food handling behaviors. | an investigation of the outbreak was initiated as a result of the number of cases of gastroenteritis reporting to a general hospital in mina during the pilgrimage to makkah (hajj). this study was conducted to identify the source of the outbreak, assess its extent, and make recommendations to prevent similar outbreaks in the future. | 2007 | 23012141 |
| evaluation of antimicrobial activity of the stem bark of cylicodiscus gabunensis (mimosaceae). | ethyl acetate (ea) extract of the stem bark of cylicodiscus gabunensis (cg) was analysed phytochemically and evaluated for its antimicrobial activity against 17 pathogenic species isolated from patient: escherichia coli, klebsiella pneumoniae, shigella dysenteriae, shigella flexneri, morganella morganii, proteus vulgaris, proteus mirabilis, salmonella typhi, citrobacter freundii, enterobacter cloacae, enterobacter agglomerans, staphylococcus aureus, streptococcus feacalis, pseudomonas aeruginosa ... | 2006 | 20162076 |
| [valuation for usefulness of selected chromosomal markers for bacillus anthracis identification. i. valuation for markers sg-749, sg-450 and sg-300]. | the article presents results of valuation for b. anthracis-specificity and usefulness for its identification obtained for different chromosomal markers. in the first part of the study markers sg-749, sg-300 and sg-450 were analyzed. for the investigation rflp-pcr and msscp techniques were used and different electrophoresis methods were tested. the results gave an information not only about specificity of tested markers but also about the possibility of shorten time necessary to obtain results of ... | 2006 | 17642312 |
| a multi-channel femtoampere-sensitivity conductometric array for biosensing applications. | rapid detection of pathogens using field deployable biosensors requires integrated sensing and data processing. detection of low concentration of biological agents is possible using accurate and real-time signal characterization devices. this paper presents a multi-channel conductometric array that can detect and measure current up to femtoampere range. the architecture uses a novel semi-synchronous sigmadelta modulation that allows measurement of ultra-small currents by using a hysteretic compa ... | 2006 | 17959433 |
| synthesis and in vitro antimicrobial studies of medicinally important novel n-alkyl and n-sulfonyl derivatives of 1-[bis(4-fluorophenyl)-methyl]piperazine. | a series of novel substituted 1-[bis(4-fluorophenyl)-methyl]piperazine derivatives (4a-g) and (5h-m) have been synthesized. the synthesized compounds were characterized by ir and 1h nmr. all the synthesized compounds were evaluated in vitro for their efficacy as antimicrobial agents against representative strains of gram-positive (staphylococcus aureus atcc 25953, streptococcus pneumoniae atcc 49619, bacillus cereus 11778, and bacillus subtilis 6051) and gram-negative bacteria (escherichia coli ... | 2006 | 16784863 |
| the enhancin-like metalloprotease from the bacillus cereus group is regulated by the pleiotropic transcriptional activator plcr but is not essential for larvicidal activity. | bacillus cereus group bacteria produce virulence factors. many of these are regulated by the pleiotropic transcriptional activator plcr, which is implicated in insect virulence. in silico analysis of the b. cereus strain atcc14579 genome showed an enhancin-like gene preceded by a typical plcr binding sequence. the gene is predicted to encode a polypeptide showing 23-25% identity with enhancins from several baculoviruses and 31% with that of yersinia pestis. viral enhancin acts after oral infecti ... | 2006 | 16790012 |
| burkholderia cenocepacia zmpb is a broad-specificity zinc metalloprotease involved in virulence. | in previous studies we characterized the burkholderia cenocepacia zmpa zinc metalloprotease. in this study, we determined that b. cenocepacia has an additional metalloprotease, which we designated zmpb. the zmpb gene is present in the same species as zmpa and was detected in b. cepacia, b. cenocepacia, b. stabilis, b. ambifaria, and b. pyrrocinia but was absent from b. multivorans, b. vietnamiensis, b. dolosa, and b. anthina. the zmpb gene was expressed, and zmpb was purified from escherichia co ... | 2006 | 16790782 |
| variability among bacillus cereus strains in spore surface properties and influence on their ability to contaminate food surface equipment. | seven strains of bacillus cereus isolated from the environment and from patients with diarrheic symptoms were examined from two angles: their spore surface properties, and their ability to adhere to stainless steel and to resist a cleaning in place (cip) procedure. our results revealed significant differences in their morphology (size of exosporium, length and number of appendages), hydrophobic character and surface protein composition. most of these proteins originated in the vegetative cell an ... | 2006 | 16793156 |
| antimicrobial activities of human beta-defensins against bacillus species. | natural defences in the human body function to protect us from numerous environmental toxins and exposure to potential harmful biological agents. an important frontline defence is antimicrobial peptides. these peptides occur at environmental interfaces and serve to limit bacterial invasion. there has been little work comparing specific peptides as potential antimicrobial compounds. in this study, we evaluated the antimicrobial activity of peptides from the human beta-defensin (hbd) family agains ... | 2006 | 16797165 |
| thermal inactivation of bacillus anthracis spores in cow's milk. | decimal reduction time (time to inactivate 90% of the population) (d) values of bacillus anthracis spores in milk ranged from 3.4 to 16.7 h at 72 degrees c and from 1.6 to 3.3 s at 112 degrees c. the calculated increase of temperature needed to reduce the d value by 90% varied from 8.7 to 11.0 degrees c, and the arrhenius activation energies ranged from 227.4 to 291.3 kj/mol. six-log-unit viability reductions were achieved at 120 degrees c for 16 s. these results suggest that a thermal process s ... | 2006 | 16751573 |
| biochemical characteristics of chitosanase from the indonesian bacillus licheniformis mb-2. | bacillus licheniformis mb-2, isolated from a hot spring water in manado, indonesia, secreted a unique chitosanase. media consisted of 0.24% chitosan, 0.25% casiton, 1% mgso4, 1.4% k2hpo4, 0.02% cacl2 x 2h2o, 0.002% feso4 x 7h2o (w/v) was used for enzyme production. purification of the enzyme through the hydrophobic interaction chromatography system (butyl sepharose 4 ff) resulted in two major active fractions; the f2 fraction was shown as a single band at both sodium dodecyl sulfate-polyacrylami ... | 2006 | 16757796 |
| [thrombophlebitis and pneumonia in hiv patient]. | 2006 | 16762297 | |
| a primary sodium pump gene of the moderate halophile halobacillus dabanensis exhibits secondary antiporter properties. | the primary sodium pump has been proved to be involved in na(+) extrusion of bacteria. in our present study, a novel gene encoding a putative primary sodium pump was cloned from chromosomal dna of moderate halophile halobacillus dabanensis d-8 by functional complementation, which expression resulted in the growth of antiporter-deficient escherichia coli strain knabc in the presence of 0.2 m nacl. the gene was sequenced and designated nap. the deduced amino acid sequence of nap has 56% identity t ... | 2006 | 16774742 |
| isolation and characterization of two potential pentachlorophenol degrading aerobic bacteria from pulp paper effluent sludge. | 2006 | 16778357 | |
| elimination and multiplication of bacteria during preparation and storage of buffy coat-derived platelet concentrates. | the prevalence of bacterial contamination of random-donor platelet concentrates (pcs) is considerably lower than that of blood donations. which key steps of the preparation procedure contribute to the elimination of bacteria was investigated. | 2006 | 16734811 |
| synthesis and evaluation of fluorogenic substrates for phospholipase d and phospholipase c. | fluorogenic analogues of phosphatidylcholine and lysophosphatidylcholine, ddpb and lysoddpb, were synthesized by an enzyme-assisted strategy. the analogues were evaluated as substrates for phospholipases c and d and lysophospholipase d. ddpb was cleaved by bacterial and plant phospholipase d (pld) enzymes and represents the first direct fluorogenic substrate for real-time measurement of pld activity. both fluorogenic substrates have potential in screening for pld and pc-plc inhibitors and for mo ... | 2006 | 16737317 |
| necrotizing gastritis due to bacillus cereus in an immunocompromised patient. | bacillus cereus is increasingly being acknowledged as a serious bacterial pathogen in immunocompromised patients. we present a case of acute necrotizing gastritis caused by b. cereus in a 37-year-old woman with acute myeloblastic leukemia, who recovered following total parenteral nutrition and treatment with imipenem and vancomycin. b. cereus was isolated from gastric mucosa and blood cultures. up to now, no case of acute necrotizing gastritis due to this organism has been reported. | 2006 | 16703301 |
| occurrence and pathogenic potential of bacillus cereus group bacteria in a sandy loam. | the major part (94%) of the bacillus cereus-like isolates from a danish sandy loam are psychrotolerant bacillus weihenstephanensis according to their ability to grow at temperatures below 7 degrees c and/or two pcr-based methods, while the remaining 6% are b. cereus. the bacillus mycoides-like isolates could also be divided into psychrotolerant and mesophilic isolates. the psychrotolerant isolates of b. mycoides could be discriminated from the mesophilic by the two pcr-based methods used to char ... | 2006 | 16710635 |
| constituents and biological activities of schinus polygamus. | the folk medicine employs schinus polygamus to treat arthritic pain and cleansing of wounds. as no reports of pharmacological studies supporting its anti-inflammatory and analgesic properties, extracts of increasing polarity were assayed on the base of fever, pain and inflammation, together with its antimicrobial activity. all the extracts showed pharmacological activities. from the most active extracts different metabolites were isolated that can in part explain the antipyretic, anti-inflammato ... | 2006 | 16713153 |
| biofilm-spore response in bacillus cereus and bacillus subtilis during nutrient limitation. | this study aimed to trace the dynamics of biofilm formation by vegetative cells and endospores of bacillus cereus dl5 and bacillus subtilis 168. counts of b. cereus dl5 and b. subtilis 168 vegetative cells and spores either attached to glass wool or, correspondingly, planktonic cells were determined by standard plate-counting methods. results from this study highlighted the biofilm-forming potential of both spores and vegetative cells of two different bacillus species. it was shown that once bac ... | 2006 | 16715822 |
| presence and growth of bacillus cereus in dehydrated potato flakes and hot-held, ready-to-eat potato products purchased in new zealand. | potato products prepared from dehydrated potato flakes have been implicated in foodborne illness incidents involving bacillus cereus intoxications. b. cereus can survive as spores in potato flakes and can germinate and multiply in the rehydrated product. this study assessed the frequency and concentration of b. cereus in dehydrated potato flakes and hot-held, ready-to-eat mashed potato products. of 50 packets of potato flakes tested, eight contained greater than 100 cfu/g b. cereus (maximum 370 ... | 2006 | 16715823 |
| lactobacillus plantarum gene clusters encoding putative cell-surface protein complexes for carbohydrate utilization are conserved in specific gram-positive bacteria. | genomes of gram-positive bacteria encode many putative cell-surface proteins, of which the majority has no known function. from the rapidly increasing number of available genome sequences it has become apparent that many cell-surface proteins are conserved, and frequently encoded in gene clusters or operons, suggesting common functions, and interactions of multiple components. | 2006 | 16723015 |
| adhesion and cytotoxicity of bacillus cereus and bacillus thuringiensis to epithelial cells are flha and plcr dependent, respectively. | some bacteria of the bacillus cereus group are enteropathogens. the first cells encountered by bacteria following oral contamination of the host are epithelial cells. we studied the capacity of these bacteria to adhere to epithelial cells and the consequences of this interaction. we found that cell adhesion is strain dependent and that a strain mutated in flha, which encodes a component of flagellum-apparatus formation, is impaired in adhesion, suggesting that flagella are important virulence fa ... | 2006 | 16697234 |
| detection of antibiotic-related genes from bacterial biocontrol agents with polymerase chain reaction. | pseudomonas chlororaphis pa23, pseudomonas spp. strain df41, and bacillus amyloliquefaciens bs6 consistently inhibit infection of canola petals by sclerotinia sclerotiorum in both greenhouse and field experiments. bacillus thuringiensis bs8, bacillus cereus l, and bacillus mycoides s have shown significant inhibition against s. sclerotiorum on plate assays. the presence of antibiotic biosynthetic or self-resistance genes in these strains was investigated with polymerase chain reaction and, in on ... | 2006 | 16699573 |
| effects of amoebae on the growth of microbes isolated from moisture-damaged buildings. | dampness, moisture, and mold in buildings are associated with adverse health outcomes. in addition to fungi and bacteria, amoebae have been found in moisture-damaged building materials. amoebae and a growing list of bacteria have been shown to have mutual effects on each other's growth, but the interactions between amoebae and microbes common in moisture-damaged buildings have not been reported. we co-cultivated the amoeba acanthamoeba polyphaga with bacteria and fungi isolated from moisture-dam ... | 2006 | 16699589 |
| probiotics for animal nutrition in the european union. regulation and safety assessment. | probiotics are alive micro-organisms, generally bacteria but also yeasts than, when ingested alive in sufficient amount, they have a positive effect on the health going beyond the nutritional ones commonly known. probiotics may operate through a nutritional and/or health or sanitary effect. micro-organisms used in animal feed in the eu are mainly bacterial strains of gram-positive bacteria belonging to the types bacillus, enterococcus, lactobacillus, pediococcus, streptococcus and strains of yea ... | 2006 | 16563585 |
| in vitro antibacterial, antifungal & cytotoxic activity of some isonicotinoylhydrazide schiff's bases and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes. | isonicotinoylhydrazide schiff's bases formed by the reaction of substituted and unsubstituted furyl-2-carboxaldehyde and thiophene-2-carboxaldehyde with isoniazid and, their co (ii), cu (ii), ni (ii) and zn (ii) complexes have been synthesized, characterized and screened for their in vitro antibacterial activity against mycobacterium tuberculosis, escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebac ... | 2006 | 16570512 |
| bacillus anthracis multiplication, persistence, and genetic exchange in the rhizosphere of grass plants. | bacillus anthracis, the causative agent of anthrax, is known for its rapid proliferation and dissemination in mammalian hosts. in contrast, little information exists regarding the lifestyle of this important pathogen outside of the host. considering that bacillus species, including close relatives of b. anthracis, are saprophytic soil organisms, we investigated the capacity of b. anthracis spores to germinate in the rhizosphere and to establish populations of vegetative cells that could support ... | 2006 | 16672454 |
| occurrence of natural bacillus thuringiensis contaminants and residues of bacillus thuringiensis-based insecticides on fresh fruits and vegetables. | a total of 128 bacillus cereus-like strains isolated from fresh fruits and vegetables for sale in retail shops in denmark were characterized. of these strains, 39% (50/128) were classified as bacillus thuringiensis on the basis of their content of cry genes determined by pcr or crystal proteins visualized by microscopy. random amplified polymorphic dna analysis and plasmid profiling indicated that 23 of the 50 b. thuringiensis strains were of the same subtype as b. thuringiensis strains used as ... | 2006 | 16672488 |
| influence of sporulation medium composition on transcription of ger operons and the germination response of spores of bacillus cereus atcc 14579. | bacillus cereus atcc 14579 endospores were produced in y1 medium, a nutrient-rich, chemically defined sporulation medium, and in modified g medium, containing low amounts of nutrients. the average transcription level of the seven ger operons per cell was 3.5 times higher in y1 medium, and the spores grown in this medium showed an enhanced germination response. | 2006 | 16672527 |
| the international bacillus anthracis, b. cereus, and b. thuringiensis conference, "bacillus-act05". | 2006 | 16672596 | |
| differential proteomic analysis of the bacillus anthracis secretome: distinct plasmid and chromosome co2-dependent cross talk mechanisms modulate extracellular proteolytic activities. | the secretomes of a virulent bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pxo1 and pxo2), cultured in rich and minimal media, were studied by a comparative proteomic approach. more than 400 protein spots, representing the products of 64 genes, were identified, and a unique pattern of protein relative abundance with respect to the presence of the virulence plasmids was revealed. in minimal medium under high co(2) tension, conditions considered to simulate th ... | 2006 | 16672610 |
| rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based system. | a rapid bead assay for detecting pathogenic bacteria with a simple microfluidic chip-based system was developed. five oligonucleotide probes corresponding to the 16s rrna of the targeted bacteria were coupled covalently to fluorescent beads. four species of bacteria (escherichia coli, salmonella enterica subsp. enterica serovar enteritidis, yersinia enterocolitica, and bacillus cereus) were used as representative food-borne pathogenic bacteria. the rnas extracted from pure cultures of these micr ... | 2006 | 16678290 |
| search for antibacterial and antifungal agents from selected indian medicinal plants. | a series of 61 indian medicinal plants belonging to 33 different families used in various infectious disorders, were screened for their antimicrobial properties. screening was carried out at 1000 and 500 microg/ml concentrations by agar dilution method against bacillus cereus var mycoides, bacillus pumilus, bacillus subtilis, bordetella bronchiseptica, micrococcus luteus, staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, streptoco ... | 2006 | 16678369 |
| rapid discrimination of cytk-1 and cytk-2 genes in bacillus cereus strains by a novel duplex pcr system. | bacillus cereus is the causative agent of gastrointestinal diarrhoea. at least three known enterotoxins may be involved in this syndrome: nonhaemolytic (nhe) enterotoxin, hbl enterotoxin and cytotoxin k. two different forms were recently described for cytotoxin k, encoded by cytk-1 and cytk-2 genes. the cytk-1 toxin appeared to carry a high toxicity, but there is currently no method available to rapidly detect and discriminate the b. cereus strains able to produce this cytk-1 form. in this study ... | 2006 | 16684105 |
| evolution of surveillance methods for detection of bacterial contamination of platelets in a university hospital, 1991 through 2004. | platelet (plt) bacterial contamination (pbc) is the most common transfusion-associated infection. it is important to understand the impact of interventions addressing this problem. | 2006 | 16686839 |
| composition of bacillus species in aerosols from 11 u.s. cities. | a pcr-based heteroduplex assay was used to determine the presence and composition of bacillus species in 11,059 environmental protection agency pm2.5 aerosol samples from 11 u.s. cities. the assay differentiated three groups: type a containing bacillus anthracis and very closely related, often pathogenic, bacillus cereus and bacillus thuringiensis strains; type b containing other b. cereus and b. thuringiensis strains; and a third group of more-distantly related bacillus species. eight of the 11 ... | 2006 | 16696702 |
| a novel ftsz-like protein is involved in replication of the anthrax toxin-encoding pxo1 plasmid in bacillus anthracis. | plasmid pxo1 encodes the tripartite anthrax toxin, which is the major virulence factor of bacillus anthracis. in spite of the important role of pxo1 in anthrax pathogenesis, very little is known about its replication and maintenance in b. anthracis. we cloned a 5-kb region of the pxo1 plasmid into an escherichia coli vector and showed that this plasmid can replicate when introduced into b. anthracis. mutational analysis showed that open reading frame 45 (repx) of pxo1 was required for the replic ... | 2006 | 16585744 |
| detailed genomic analysis of the wbeta and gamma phages infecting bacillus anthracis: implications for evolution of environmental fitness and antibiotic resistance. | phage-mediated lysis has been an essential laboratory tool for rapidly identifying bacillus anthracis for more than 40 years, relying on the gamma phage derivative of a bacillus cereus prophage called w. the complete genomic sequences of the temperate w phage, referred to as wbeta, and its lytic variant gamma were determined and found to encode 53 open reading frames each, spanning 40,864 bp and 37,373 bp, respectively. direct comparison of the genomes showed that gamma evolved through mutations ... | 2006 | 16585764 |
| levels of glycine betaine in growing cells and spores of bacillus species and lack of effect of glycine betaine on dormant spore resistance. | bacteria of various bacillus species are able to grow in media with very high osmotic strength in part due to the accumulation of low-molecular-weight osmolytes such as glycine betaine (gb). cells of bacillus species grown in rich and minimal media contained low levels of gb, but gb levels were 4- to 60-fold higher in cells grown in media with high salt. gb levels in bacillus subtilis cells grown in minimal medium were increased approximately 7-fold by gb in the medium and 60-fold by gb plus hig ... | 2006 | 16585779 |
| structural basis of the sphingomyelin phosphodiesterase activity in neutral sphingomyelinase from bacillus cereus. | sphingomyelinase (smase) from bacillus cereus (bc-smase) hydrolyzes sphingomyelin to phosphocholine and ceramide in a divalent metal ion-dependent manner. bc-smase is a homologue of mammalian neutral smase (nsmase) and mimics the actions of the endogenous mammalian nsmase in causing differentiation, development, aging, and apoptosis. thus bc-smase may be a good model for the poorly characterized mammalian nsmase. the metal ion activation of sphingomyelinase activity of bc-smase was in the order ... | 2006 | 16595670 |
| structure of phage protein bc1872 from bacillus cereus, a singleton with new fold. | 2006 | 16596646 | |
| the bacillus anthracis chromosome contains four conserved, excision-proficient, putative prophages. | bacillus anthracis is considered to be a recently emerged clone within the bacillus cereus sensu lato group. the b. anthracis genome sequence contains four putative lambdoid prophages. we undertook this study in order to understand whether the four prophages are unique to b. anthracis and whether they produce active phages. | 2006 | 16600039 |
| antimicrobial, wound healing and antioxidant activity of plagiochasma appendiculatum lehm. et lind. | plagiochasma appendiculatum (aytoniaceae) of the order marchantiales is widely used in the form of paste ethnomedicinally by gaddi tribe in kangra valley for treating skin diseases. in this context, antimicrobical potential of plagiochasma appendiculatum against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing activity. th ... | 2006 | 16600543 |
| bioactive principles in the bark of pilidiostigma tropicum. | the crude dichloromethane bark extract of pilidiostigma tropicum (myrtaceae) from north queensland, australia, shows antibacterial and cytotoxic activity. bioactivity-directed separation led to the isolation of rhodomyrtoxin b and ursolic acid-3-p-coumarate as the biologically active materials. the structures of these compounds were elucidated on the basis of spectral analysis. the intercalation interaction of rhodomyrtoxin b with dna was investigated using molecular mechanics and ab initio mole ... | 2006 | 16601968 |
| growth of escherichia coli, pichia pastoris and bacillus cereus in the presence of the ionic liquids [bmim][bf4] and [bmim][pf6] and organic solvents. | the influence of the two most commonly used ionic liquids (1-butyl-3-methyl imidazolium tetrafluoroborate, [bmim][bf(4)], 1-butyl-3-methyl imidazolium hexafluorophosphate, [bmim][pf(6)]) and three selected organic solvents (dimethylsulfoxide, ethanol, methanol) on the growth of escherichia coli, pichia pastoris and bacillus cereus was investigated. [bmim][bf(4)] was toxic at 1% (v/v) on all three microorganisms. the minimal inhibitory concentration (mic) of [bmim][bf(4)] on e. coli growth was be ... | 2006 | 16614927 |
| antimicrobial activity of saponins from medicago sp.: structure-activity relationship. | the antimicrobial activity of saponins from medicago sativa, m. arborea and m. arabica against a selection of medically important yeasts, gram-positive and -negative bacteria was investigated. structure-activity growth inhibitory effects of related prosapogenins and sapogenins are also described. increasing antibiotic activity was observed going from the saponin extracts to the sapogenin samples, suggesting that the sugar moiety is not important for the antimicrobial efficacy. activity was espec ... | 2006 | 16619355 |
| pathogenomic sequence analysis of bacillus cereus and bacillus thuringiensis isolates closely related to bacillus anthracis. | bacillus anthracis, bacillus cereus, and bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the b. cereus sensu lato group. while independently derived strains of b. anthracis reveal conspicuous sequence homogeneity, environmental isolates of b. cereus and b. thuringiensis exhibit extensive genetic diversity. here we report the sequencing and comparative analysis of the genomes of two members of the b. cereus group, b. thuringiensis 97-27 subsp. konkukian serotyp ... | 2006 | 16621833 |
| influence of type of food on the kinetics and overall production of bacillus cereus emetic toxin. | potato puree and penne pasta were inoculated with cereulide producing b. cereus 5964a and b. cereus ns117. static incubation at 28 degrees c proved these two foods to be a better substrate for higher cereulide production (4,080 ng/g in puree and 3,200 ng/g in penne were produced by b. cereus 5964a during 48 h of incubation) compared with boiled rice (2,000 ng/g). this difference occurred despite b. cereus counts of more than 10(8) cfu/g in all three products. aeration of cultures had a negative ... | 2006 | 16629028 |
| cyt2ba of bacillus thuringiensis israelensis: activation by putative endogenous protease. | the gene cyt2ba of bacillus thuringiensis subsp. israelensis was cloned for expression, together with p20, in an acrystalliferous strain. the large hexagonal crystals formed were composed of cyt2ba, which facilitated its purification. crystal solubilization in the presence of endogenous proteases (with spores and cell debris) enabled quick and simple procedure to obtain rather pure and active toxin species by cleavage between amino acid residues 34 and 35, most likely by a camelysin-like proteas ... | 2006 | 16630537 |
| bacterial contamination of nonsterile disposable gloves before use. | after bacillus cereus recovery in opened boxes of disposable gloves, the bacteriological contamination of disposable nonsterile gloves kept stored in native packages was investigated prospectively. | 2006 | 16630975 |
| fluorescence quencher improves scansystem for rapid bacterial detection. | the optimized scansystem could detect contaminated platelet products within 24 h. however, the system's sensitivity was reduced by a high fluorescence background even in sterile samples, which led to the necessity of a well-trained staff for confirmation of microscope results. | 2006 | 16635069 |
| glycosylation of flavonoids with a glycosyltransferase from bacillus cereus. | microbial glycosyltransferases can convert many small lipophilic compounds such as phenolics, terpenoids, cyanohydrins and alkaloids into glycons using uridine-diphosphate-activated sugars. the main chemical functions of glycosylation processes are stabilization, detoxification and solubilization of the substrates. the gene encoding the udp-glycosyltransferase from bacillus cereus, bcgt-1, was cloned by pcr and sequenced. bcgt-1 was expressed in escherichia coli bl21 (de3) with a his-tag and pur ... | 2006 | 16640583 |