Publications

emergence of bactericidal and opsonizing antibody to vibrio vulnificus following bacterial infection.	virulent isolates of vibrio vulnificus resist the bactericidal and opsonizing effects of normal human serum, in contrast to environmental isolates, which are highly serum susceptible. immune responses to bacteremic v. vulnificus infections in human subjects have not been characterized. serum from a patient who survived sepsis caused by v. vulnificus had substantial bactericidal and opsonizing immunoglobulin g (igg) for his own bloodstream isolate. killing was mediated by the classical complement ...	1986	3958138
serotonin and 5-hydroxyindoleacetic acid in brains of suicide victims. comparison in chronic schizophrenic patients with suicide as cause of death.	serotonin (5-ht) and 5-hydroxyindoleacetic acid concentrations were determined in various brain areas of nonschizophrenic suicide victims, chronic schizophrenic patients with or without suicide as the cause of death, and normal control subjects without psychiatric or neurologic disorders. serotonin concentrations in the basal ganglia were significantly elevated in suicide victims and chronic schizophrenic patients, as were 5-hydroxyindoleacetic acid concentrations in the occipital cortex. these ...	1986	2423050
identification and characterization of the nmyc gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities.	increased n-myc (now designated nmyc in human gene nomenclature) gene expression has been detected at the transcriptional level in certain types of neoplasms. as yet, the n-myc gene product has not been identified. to detect and characterize the n-myc gene product, we have developed monoclonal antibodies against the putative n-myc gene product made in escherichia coli as a fusion protein. the antibodies that recognize the n-myc-specific regions were selected on the basis of their reactivities to ...	1986	2426708
[effects of enzymatic deglycosylation of human goiter thyroglobulin on its immunochemical properties].	thyroglobulin (tg), isolated from soluble iodoproteins by ammonium sulphate fractionation, was enzymatically deglycosylated in vitro and analyzed by polyacrylamide gel electrophoresis, double immunodiffusion and non-commercial ria. carbohydrate and iodine content was chemically determined. by paage deglycosylated tg (dtg) showed the appearance of a major band in the 12s region and three slower migrating bands corresponding to higher aggregates than 19s tg. in immunodiffusion by testing native an ...	1986	2430684
susceptibility to varicella-zoster virus among adults at high risk for exposure.	the adult health care provider who is susceptible to varicella zoster virus (vzv) represents a risk to her or himself and to patients. nineteen percent of employees at this children's hospital had no or uncertain prior experience with vzv, and of these, 28% were found to be vzv susceptible, representing 5% of the total population of 2,730 hospital employees. during the 12 months of study, six of the potential 135 to 137 vzv-susceptible individuals acquired varicella. vzv-susceptible health care ...	1986	3019915
susceptibility to varicella-zoster virus among adults at high risk for exposure.	the adult health care provider who is susceptible to varicella zoster virus (vzv) represents a risk to her or himself and to patients. nineteen percent of employees at this children's hospital had no or uncertain prior experience with vzv, and of these, 28% were found to be vzv susceptible, representing 5% of the total population of 2,730 hospital employees. during the 12 months of study, six of the potential 135 to 137 vzv-susceptible individuals acquired varicella. vzv-susceptible health care ...	1986	3019915
carbachol- and norepinephrine-stimulated phosphoinositide metabolism in rat brain: effect of chronic cholinesterase inhibition.	activation of cholinergic muscarinic receptors leads to several biochemical events including an increased turnover of phosphoinositides. in this study we have investigated whether repeated administration of the organophosphorus insecticide disulfoton, known to cause the development of tolerance to this compound, would affect phosphoinositide metabolism in rat brain. basal and carbachol-stimulated phosphoinositide metabolism were measured in cerebral cortex slices, by measuring the accumulation o ...	1986	3020234
carbachol- and norepinephrine-stimulated phosphoinositide metabolism in rat brain: effect of chronic cholinesterase inhibition.	activation of cholinergic muscarinic receptors leads to several biochemical events including an increased turnover of phosphoinositides. in this study we have investigated whether repeated administration of the organophosphorus insecticide disulfoton, known to cause the development of tolerance to this compound, would affect phosphoinositide metabolism in rat brain. basal and carbachol-stimulated phosphoinositide metabolism were measured in cerebral cortex slices, by measuring the accumulation o ...	1986	3020234
mapping of the class ii region of the human major histocompatibility complex by pulsed-field gel electrophoresis.	the class ii region of the human major histocompatibility complex (mhc) encodes a polymorphic set of cell surface glycoproteins involved in the regulation of the immune response. each glycoprotein is a heterodimer composed of a alpha-chain of relative molecular mass (mr) 34,000 (34 k) and a beta-chain of mr = 28k. the products of the class ii region have been characterized by the mixed lymphocyte reaction, serology, primed lymphocyte typing and dna cloning. dr, dq and dp, three subregions contai ...	1986	3020430
mapping of the class ii region of the human major histocompatibility complex by pulsed-field gel electrophoresis.	the class ii region of the human major histocompatibility complex (mhc) encodes a polymorphic set of cell surface glycoproteins involved in the regulation of the immune response. each glycoprotein is a heterodimer composed of a alpha-chain of relative molecular mass (mr) 34,000 (34 k) and a beta-chain of mr = 28k. the products of the class ii region have been characterized by the mixed lymphocyte reaction, serology, primed lymphocyte typing and dna cloning. dr, dq and dp, three subregions contai ...	1986	3020430
production of platelet-derived growth factor-like molecules by cultured arterial smooth muscle cells accompanies proliferation after arterial injury.	the migration and proliferation of smooth muscle cells (smcs) within the intima of arteries following mechanical injury is thought to be initiated by vessel wall injury and release of growth factors, in particular the platelet-derived growth factor (pdgf). however, the mechanism by which smc proliferation is regulated after platelet interaction with the vessel wall has ceased is unknown. here we show that smcs derived from the intima of injured rat arteries (intimal smcs) are phenotypically dist ...	1986	3020543
production of platelet-derived growth factor-like molecules by cultured arterial smooth muscle cells accompanies proliferation after arterial injury.	the migration and proliferation of smooth muscle cells (smcs) within the intima of arteries following mechanical injury is thought to be initiated by vessel wall injury and release of growth factors, in particular the platelet-derived growth factor (pdgf). however, the mechanism by which smc proliferation is regulated after platelet interaction with the vessel wall has ceased is unknown. here we show that smcs derived from the intima of injured rat arteries (intimal smcs) are phenotypically dist ...	1986	3020543
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.	two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...	1986	3020551
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.	two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...	1986	3020551
exposure of sheep to natural aerosols of foot-and-mouth disease virus.	sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ...	1986	3020658
[structure and functions of cloned adenovirus dna].		1986	3020801
subtypes and functions of alpha-adrenoceptors.		1986	3020944
thymidine kinase with altered substrate specificity of acyclovir resistant varicella-zoster virus.	the acyclovir resistant mutant of varicella-zoster virus acv-r (a 8) induced the same level of thymidine kinase activity in infected cells as the parent kawaguchi strain. however, it induced less deoxycytidine kinase activity and did not induce phosphorylating activity for the nucleotide analogue, 9-(2 hydroxy-ethoxymethyl)-guanine-(acyclovir). another acyclovir resistant mutant, acv-r (a 4), which is cross-resistant to phosphonoacetate and is thought to be a viral dna polymerase mutant, induced ...	1986	3022711
identification and characterization of mg2+-dependent phosphotyrosyl protein phosphatase from rat liver cytosol.	although highly purified preparations of mg2+-dependent phosphoseryl protein phosphatase (also designated phosphatase ia or phosphatase 2c) dephosphorylated phosphotyrosyl histone, the activity has been resolved from phosphatase ia by polyacrylamide gel electrophoresis at ph 9.5. this novel phosphotyrosyl-specific protein phosphatase absolutely requires mg2+ or mn2+ for activity, is inhibited by zn2+, vanadate and fluoride, and has an optimal ph of 9.0 and mr = 50,000. certain properties of this ...	1986	3022716
dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility.	in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ...	1986	2942039
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.	physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...	1986	2946570
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.	physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...	1986	2946570
ultrastructural changes in the sarcoplasmic reticulum in acute myocardial ischemia.	we investigated the morphologic changes in the sarcoplasmic reticulum (sr) in acute myocardial ischemia, induced by occlusion of the coronary artery in the canine heart, by freeze-fracture of sr in situ and in vitro, and they were compared to the alterations in ca++-stimulated atpase activity and protein composition of the isolated sr. both sr in situ and the isolated sr exhibited typical intramembranous particles with diameters of 70 to 90 a in freeze-fracture replicas. the intramembranous part ...	1986	2948034
synthesis and quantitative structure-activity relationships of antiallergic 2-hydroxy-n-1h-tetrazol-5-ylbenzamides and n-(2-hydroxyphenyl)-1h-tetrazole-5-carboxamides.	the synthesis and antiallergic activity of a series of 2-hydroxy-n-1h-tetrazol-5-ylbenzamides and isomeric n-(2-hydroxyphenyl)-1h-tetrazole-5-carboxamides is described. a relationship between structure and intravenous antiallergic activity in the rat passive cutaneous anaphylaxis (pca) test has been established using a hansch/free-wilson model and used to direct studies toward potent derivatives. the contribution of physicochemical properties to activity is discussed. one member of this series, ...	1986	2870188
report of a wound infection caused by vibrio parahaemolyticus and vibrio vulnificus.	the present case describes a foot wound caused by a clam shell from which both vibrio parahaemolyticus and vibrio vulnificus were recovered. although extraintestinal infections associated with vibrio parahaemolyticus have been reported previously, the simultaneous isolation of two marine vibrios from our case suggests that these organisms may coexist in mixed infections from a common source.	1986	2875824
effects of somatostatin and sms 201-995 on carbohydrate metabolism in normal man.	growth hormone (gh) is important in diabetes in view of its anti-insulin actions and its relation to the long-term complications of the disease. the suppression of gh secretion in diabetics has theoretical and possible therapeutic interest. native somatostatin has multiple actions, including inhibition of the secretion of insulin, glucagon, thyroid-stimulating hormone (tsh), and various gut hormones. it also has inhibitory effects on gastrointestinal motility, exocrine secretion, nutrient absorp ...	1986	2876501
[human gingival pigmentation due to electrochemical degradation. immunological study of biopsies using monoclonal antibodies].		1986	3464603
composition of major outer membrane proteins of vibrio vulnificus isolates: effect of different growth media and iron deficiency.	the outer membrane proteins of vibrio vulnificus including isolates from humans, seawater and an asari clam were examined by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis. a major outer membrane protein with an apparent molecular weight of 48,000 (48k protein) was common to all the strains grown in 3% nacl-nutrient broth; however this 48k protein was not produced in any of the strains grown in chemically defined medium. other major outer membrane proteins with molecular weights ...	1986	3724558
virulence of vibrio vulnificus strains from marine environments.	vibrio vulnificus strains isolated from geographically diverse marine sources were compared with clinical isolates for phenotype and in vitro and in vivo production of virulence factors. there were no differences between environmental and clinical strains on the basis of biochemical characteristics or antimicrobial susceptibility patterns. cytolysin and cytotoxin titers produced by environmental strains were generally comparable to those of clinical strains. of 29 environmental isolates tested, ...	1986	3729387
plasmid carriage in vibrio vulnificus and other lactose-fermenting marine vibrios.	a total of 42 clinical and environmental isolates of vibrio vulnificus were examined for plasmid carriage. of these, only five (12%) harbored plasmids, which were of various molecular weights. in contrast, 20 of 32 (62.5%) unidentified lactose-fermenting vibrio spp. were found to possess plasmids with masses of 2.1 to 150 megadaltons. in these isolates, multiple plasmids were common, with an average of 2.25 plasmids per plasmid-containing strain. attempts to demonstrate a correlation with the pl ...	1986	3729404
purification and initial characterization of the 71-kilodalton rat heat-shock protein and its cognate as fatty acid binding proteins.	the major rat heat-shock (stress) protein and its cognate were purified to electrophoretic homogeneity from livers of heat-shocked rats. both proteins exhibited similar behavior on a variety of column chromatography matrices but were separable by preparative isoelectric focusing under nondenaturing conditions by virtue of a 0.2 ph unit difference in isoelectric point. both purified proteins had similar physical properties, suggesting the possibility that they may have similar biological function ...	1986	3730359
preparation, isolation and characterization of mouse ige monoclonal antibodies against trichosanthin protein.		1986	3739501
a reappraisal of the complement fixation test using soluble mycobacterium avium antigen for the detection of m. paratuberculosis infection in cattle.	serums from 263 cattle suspected of having paratuberculosis on the basis of clinical signs, were tested for antibodies to mycobacterium paratuberculosis with a complement fixation test (cft) employing a heat extracted, soluble m. avium antigen. microscopic examination confirmed that 172 (65.4%) clinically affected animals had paratuberculosis, the remainder being disease-free. the specificity and sensitivity of the cft was 92.3% and 74.4% respectively. phenol treatment of serums before testing w ...	1986	3753338
calmodulin gene expression during sea urchin development: persistence of a prevalent maternal protein.	calmodulin gene expression during embryogenesis of the sea urchin strongylocentrotus purpuratus was investigated. several identical bacteriophages containing a cdna insert encoding sea urchin calmodulin (cm-1) were identified by screening a lambda gt10 library of s. purpuratus gastrula-stage cdnas with a chicken calmodulin cdna sequence. a 1.2-kb cdna fragment from cm-1 was subcloned into puc-8 to give plasmid pcal-8. pcal-8 contains a single open reading frame encoding 79 amino acids, a termina ...	1986	3753945
studies on cardiac distribution and function of neuropeptide y.	high concentrations of a novel peptide, neuropeptide y, have been demonstrated in the guinea-pig and canine heart and in the latter, a particularly high concentration was found in the region of the coronary vasculature (126 +/- 31 pmol g-1). intra-arterial infusion of neuropeptide y for 30 s into the coronary artery of the intact, innervated dog heart resulted in a rapid and short-lasting reduction of blood flow from 38 +/- 4 to 31 +/- 3 ml min-1 (p less than 0.05) to resume control level, 39 +/ ...	1986	3754377
effect of alterations in lipid packing order by hydrophobic solutes on the association state of protein assemblies in model membranes.	the effects of two hydrophobic solutes which perturb lipid packing order, permethrin and allethrin, on the aggregated state of a lipid membrane-incorporated protein, bacteriorhodopsin (br), have been determined by resonance energy transfer measurements. as temperature is increased from well below the main gel-fluid phase transition temperature (tc) of the lipid, patches of aggregated br dissociate into monomers, a few degrees below the tc (m.p. heyn, a. blume, m. rehorek and n.a. dencher, bioche ...	1986	3754778
stabilization of intracellular recordings in the cat spinal cord using high-frequency ventilation.	stabilization of intracellular recordings by a substantial reduction of respiratory motion may be accomplished by the use of high-frequency ventilation (600-800 breaths/min). the technique is a simple one and enables maintenance of relatively constant blood gas tensions. although the improved stability was demonstrated in experiments performed on alpha motoneurones in the thoracic spinal cord, it is anticipated that a reduced respiratory movement should benefit recordings in other spinal and sup ...	1986	3756629
breath hydrogen concentration and small intestinal malabsorption in calves.	breath hydrogen concentrations were measured to assess intestinal carbohydrate malabsorption in preruminating calves. oral administration of 1.25 g of lactulose (a nonabsorbable carbohydrate)/kg to calves produced breath hydrogen concentrations significantly (p less than 0.001) higher than values determined after calves were fed milk and before the treatment was given. this indicates that, in the calf, fermentation of nonabsorbed carbohydrates results in increased breath hydrogen values. to indu ...	1986	3767107
sensitive analytical method for serum primidone and its active metabolites for single-dose pharmacokinetic analysis in human subjects.		1986	3769107
participation of iron on the growth inhibition of pathogenic strains of mycobacterium avium and m. paratuberculosis in serum.	serum, which is low in freely-available iron, was bacteriostatic for m. avium and m. paratuberculosis irrespective of the ability of these organisms to produce the iron-chelators mycobactin and exochelin for iron acquisition. bacteriostasis was overcome when the serum was supplemented with exochelin or mycobactin, but only if the mycobacteria had previously adapted to growth in a low iron environment. the results imply that for studies on mycobacterial pathogenicity the test cultures should be g ...	1986	3788345
isolation and characterization of a mucin-degrading strain of peptostreptococcus from rat intestinal tract.	a mucin-degrading microorganism was isolated from the intestinal tract by serial sectioning from the serosal side of the caecum wall from a conventional rat. the ability of degrading the intestinal water-soluble mucin was present both in vivo after monocontamination of germ-free rats and in vitro, when adding the microbe to mucin medium. the morphology, gram-positive cocci single or in short chains and the very weak biochemical activities allow us to place this strain in the species peptostrepto ...	1986	3788570
bioluminescence in a strain of the human pathogenic bacterium vibrio vulnificus.	we report the existence of a bioluminescent strain of the human pathogen vibrio vulnificus. the isolate was obtained from blood following a fatal wound infection and thus represents the first description of an infection caused by a luminescent bacterium.	1986	3789716
rapid serological identification of vibrio vulnificus by anti-h coagglutination.	staphylococcus aureus cowan 1 cells were armed with anti-flagellar (anti-h) antibody produced in rabbits immunized with flagellar core protein prepared from vibrio vulnificus. this reagent was assessed by coagglutination for its capacity to agglutinate and identify v. vulnificus. a species-specific h antigen is expressed in the core proteins of the polar flagella of v. vulnificus. of 435 v. vulnificus isolates identified bacteriologically, 432 (99.3%) were agglutinated in the slide test within 2 ...	1986	3789720
comparison of histones in normal and xeroderma pigmentosum lymphoblastoid cells.	histones from normal human and xeroderma pigmentosum, complementation group a (xpa), lymphoblastoid cells were compared both quantitatively, qualitatively and for binding affinity for dna. electrophoretic examination of the histones showed that all five major histone species were present in both cell groups and that there were no quantitative differences between normal and xpa histones. binding affinity to [3h] mammalian dna of the histones was determined. no significant differences were observe ...	1986	3791423
surgical management of peritoneal dialysis catheters in children: five-year experience with 1,800 patient-month follow-up.	currently at our institution more than 90% of the children with end-stage renal disease are managed with continuous ambulatory peritoneal dialysis (capd) in preference to hemodialysis until a successful transplant is accomplished. recent refinements in capd catheters and dialysis techniques have greatly added to the many medical, psychological, and economic advantages of capd compared with chronic hemodialysis. ninety-three patients less than 21 years of age underwent insertion of 167 peritoneal ...	1986	3794985
[a case of vibrio vulnificus infection].		1986	3795561
[changes in the characteristics of glucocorticoid hormone binding in human peripheral blood lymphocytes during myocardial infarct].	competitive binding was used to determine the number of binding sites to glucocorticoid hormones in peripheral blood leukocytes of normal subjects, patients with unstable angina pectoris and patients with myocardial infarction. a sharp (2-3-fold) increase in the number of binding sites was observed in patients with myocardial infarction during the first 24 hours after the onset of the disease.	1986	3801148
[mast cell-affinity lymphocytes as indicator cells of the forms of hemoblastoses].	the formation of mastolymphocyte rosettes was used to determine that in chronic lymphocytic leukemia and bovine leukemia, unlike healthy people and animals as well as patients with solid tumours of different localization and hodgkin's disease, a large amount of cells appear among peripheral blood lymphocytes having membrane affinity for mast cells. this may be used as an additional criterion in laboratory diagnosis of these diseases.	1986	3485042
experience with isoxicam and catabolin.	1 synovial cells produce a protein factor, catabolin, that induces chondrocytes to resorb their own matrix with the release of glycosaminoglycan (gag). 2 studies were performed to determine the effect of isoxicam, a new nsaid, on gag production in a catabolin system utilizing tissue culture of bovine nasal septum in a medium containing pooled synovial tissue from young pigs. 3 using fresh medium containing no synovial tissue, the release of gag by inherent cartilage breakdown was 49.7% after 8 d ...	1986	3497659
second-generation cephalosporins.		1986	3081544
identification of n epsilon-carboxymethyllysine as a degradation product of fructoselysine in glycated protein.	the chemistry of maillard or browning reactions of glycated proteins was studied using the model compound, n alpha-formyl-n epsilon-fructoselysine (ffl), an analog of glycated lysine residues in protein. incubation of ffl (15 mm) at physiological ph and temperature in 0.2 m phosphate buffer resulted in formation of n epsilon-carboxymethyllysine (cml) in about 40% yield after 15 days. cml was formed by oxidative cleavage of ffl between c-2 and c-3 of the carbohydrate chain and erythronic acid (ea ...	1986	3082871
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.	lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...	1986	3085110
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.	lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...	1986	3085110
effect of nifedipine on cerebral high-energy phosphates after cardiac arrest and resuscitation in the rat.	we studied the effect of nifedipine, a calcium entry blocker, on the recovery of cerebral adenosine triphosphate (atp), creatine phosphate (cp), and lactate levels following resuscitation from cardiac arrest. using the cardiac arrest and resuscitation model of de garavilla, babbs, and tacker with an arrest time of eight minutes, 76% of the animals arrested were resuscitated with an average intermittent abdominal compression-cpr time of 3.3 minutes. rats were assigned randomly to the following gr ...	1986	3085559
evidence that some membrane ligands modulate the plasmalemma fluidity of endothelial cells in culture.	the effect of some membrane ligands on the plasmalemmal fluidity of endothelial cells from bovine aorta in culture was investigated. the ligands used were: cationic ferritin (pi 8.5), soybean agglutinin, concanavalin a, wheat germ agglutinin, as well as glutaraldehyde at different concentrations. the fluidity probe employed was 1,6-diphenyl-1, 3, 5-hexatriene (dph) and the parameter determined to quantify the fluidity was fluorescence steady-state anisotropy. the optimum time interval required b ...	1986	3088620
nasopharyngeal carriage of neisseria meningitidis in the contacts of index cases of meningococcal meningitis.		1986	3091676
[a saved case of vibrio vulnificus infection with shock, dic and necrotizing fasciitis].		1986	3097209
protamine sulfate-induced enzyme secretion from rabbit neutrophils.	protamine sulfate induces enzyme secretion from rabbit neutrophils. enzyme secretion is mainly due to exocytosis but, depending on the experimental conditions, a small amount of cytolysis may occur. as compared with stimulation of neutrophil functions by other activators, protamine sulfate-induced enzyme release by exocytosis is a relatively slow process and is not accompanied by a marked activation of the metabolic burst. for optimal exocytosis, extracellular ca2+ is required, but there is stil ...	1986	3098680
non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains.	the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ...	1985	3156400
survival of smallpox virus.		1985	2857360
benzodiazepines enhance the muscimol-dependent activation of phospholipase a2 in glioma c6 cells.	glioma c6 cells were incubated with [14c]arachidonate to label membrane phospholipids. muscimol, a selective gamma-aminobutyric acid a receptor agonist, but not (-)-baclofen, a selective gamma-aminobutyric acid b receptor agonist, stimulates [14c]arachidonate release from c6 cells as a result of hydrolysis of a small pool of phosphatidylcholine and phosphatidylethanolamine by phospholipase a2. this release is facilitated by diazepam and a number of other benzodiazepines such as flunitrazepam, me ...	1985	2857784
immunochemical recognition of oxytocin by antiserum to l-prolyl-leucyl-glycine amide.	antisera were raised in rabbits against pro-leu-gly-nh2 (plg)-bovine thyroglobulin conjugates. specificity of the antisera towards oxytocin was evaluated by a radioimmunoassay as well as by immunohistochemical procedures on hypothalamic tissue and hormone bound to sepharose beads. it was concluded that immunization with plg can raise antisera that are specific to oxytocin.	1985	2859563
lymphatic transport of sodium cefoxitin in the presence of sodium 5-methoxysalicylate after injection into rat rectal connective tissue, femoral muscle and femoral vein.	lymphatic uptake of sodium cefoxitin after injection into rectal connective tissue was greater than after injection into the femoral muscle of rats. coadministration with sodium 5-methoxysalicylate enhanced lymphatic drug uptake at both sites. this enhancement may be an indirect result of 5-methoxysalicylate's suppression of vascular permeation of the cefoxitin. an adjuvant-induced increase in lymphatic fluid flow may also be partially involved in the enhancement of cefoxitin lymphatic transport ...	1985	2863362
[physiological and pharmacologic studies of the adrenal medulla by the measurement of plasma catecholamine levels].	the new techniques for the assay of the plasma catecholamines: noradrenaline, dopamine and, most importantly, adrenaline, because of their sensitivity and precision, have provided new information about the physiology and the pharmacology of the adrenal medulla in animals and in man. the most effective of these new techniques are the radioenzymatic techniques. the activation of the adrenal medulla in the dog or the rat appears to be frequency-dependent by maintaining a fixed concentration ratio i ...	1985	2863423
hypotensive effects after topical and intra-arterial administration of alpha-adrenergic agonists in isolated perfused rabbit eyes.	the influence of nonselective and selective alpha-adrenergic agonists on intraocular pressure (iop) was studied in isolated arterially perfused rabbit eyes. drugs were given intra-arterially or topically. with either route of administration, no initial hypertensive phase was observed after l-epinephrine, l-norepinephrine, l-phenylephrine (alpha 1), or methoxamine (alpha 1), but there was an immediate ocular hypotensive effect. the alpha 2-agonist b-ht 920 caused a nonsignificant 1 mm hg fall in ...	1985	2866480
neural and pavlovian influences on immunity.	the traditional view that the nervous and immune systems are functionally independent (aside from general stress effects and autoimmune disorders of the nervous system) is being challenged by a new view that the nervous system regulates the activity of the immune system. if this is true, it should be possible to change the activity of the immune system by means of pavlovian conditioning, just as it is possible to condition other physiological events influenced by the autonomic nervous system or ...	1985	2866484
restriction nuclease digestions driven to completion by escherichia coli rna polymerase and t4 gene 32 protein.	restriction enzyme digestions of large scale dna preparations often do not go to completion. this is due to product inhibition by the newly generated ends of the digested dna. the addition of exogenous proteins that bind tightly to the free ends of dna or to single-stranded dna will relieve this inhibition. we show that a considerable savings on restriction nucleases can be attained by the addition of rna polymerase or t4 gene 32 protein in stoichiometric amounts to the newly produced dna ends.	1985	2981211
herpes simplex virus and cytomegalovirus infections in burned patients.	herpesvirus infections are commonly seen in immunosuppressed patients and may account for considerable morbidity and some mortality. we prospectively studied 52 patients with severe burn injuries in order to determine the prevalence of viral infections in this group of patients. serologic testing was done each week to diagnose primary and reactivation infections. twenty-seven of 52 patients (52%) became infected with either herpes simplex virus (hsv) or cytomegalovirus (cmv) or both. hsv infecti ...	1985	2981325
conditioned taste aversion suppresses induction of delayed-type hypersensitivity immune reactions.	conditioned taste aversion was induced in mice by pairing saccharin drinking with an intraperitoneal injection of lithium chloride, a toxic but nonimmunosuppressive drug. conditioned mice showed not only suppressed saccharin drinking but also a 75% reduction in the induction of delayed-type hypersensitivity immune responses to low doses of sheep erythrocytes. this effect was observed with doses of lithium chloride which had no effect of their own on immune functions. in addition, a reduction in ...	1985	2987987
inflammatory myopathies: part 2.		1985	2988409
receptors for epidermal growth factor in the rat uterus.	uterine membranes contain high affinity [dissociation constant (kd) = 0.36 nm], saturable binding sites for [125i]iodo-epidermal growth factor (egf). the binding of [125i] iodo-egf is specific since it is abolished by excess unlabeled egf but not by excess unlabeled insulin, fibroblast growth factor, or multiplication-stimulating activity. incubation of [125i] iodo-egf with uterine membranes, followed by chemical cross-linking with disuccinimidyl suberate and detergent extraction reveals a major ...	1985	2988913
the biologic significance of the mixed lymphocyte kidney culture in humans.	the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ...	1985	2996186
evaluation of a photolabile derivative of 1,25-dihydroxyvitamin d3 as a photoaffinity probe for 1,25-dihydroxyvitamin-d3 receptor in chick intestinal cytosol.	we evaluated the viability of 1 alpha, 25-dihydroxyvitamin d3-3 beta-[n-(4-azido-2-nitrophenyl)glycinate] (1,25-(oh)2-d3-ang), an analog of 1 alpha, 25-dihydroxyvitamin d3 (1,25-(oh)2-d3) as a photoaffinity probe for 1,25-(oh)2-d3 receptor in chick intestinal cytosol. a competitive-binding assay revealed that chick intestinal cytosolic 1,25-(oh)2- d3 receptor bound to 1,25-(oh)2-d3-ang approximately 20-times less effectively than it did to 1,25-(oh)2-d3. irradiation of 1,25-(oh)2-d3- ang in the ...	1985	2998355
effect of pyrimidinone treatment on lethal and immunosuppressive murine cytomegalovirus infection.	the 2-amino-5-halo-pyrimidinones, which are potent interferon inducers and antiviral agents, were found to be protective against lethal cytomegalovirus (cmv) challenge in weanling or neonatal mice when administered before virus challenge. this protection was dependent upon the dosage of pyrimidinone administered. weanling mice infected with a sublethal challenge of cmv exhibited moderate to severe immunosuppression as measured by reduced splenic cell blastogenic responses in vitro to the mitogen ...	1985	3000290
dimethylthiourea consumption reflects h2o2 concentrations and severity of acute lung injury.	even though dimethylthiourea (dmtu) effectively scavenges o2 metabolites in vitro, it is often unclear if scavenging of o2 metabolites is the mechanism by which dmtu decreases tissue injury in biological models. since dmtu not only scavenges o2 metabolites but is also consumed in a dose-response manner following reaction with hydrogen peroxide (h2o2) in vitro, we wondered whether dmtu would also be consumed by o2 metabolites in biological systems and if dmtu consumption would then reflect o2 met ...	1985	3001019
relation of capsular materials and colony opacity to virulence of vibrio vulnificus.	colonies which varied in opacity were isolated from the four strains of vibrio vulnificus. opaque and translucent colonial types of the strains were distinguished from the corresponding parent strains. variation in the opacity of colonies formed by each strain was accompanied by variation of capsular material formation, which was clarified by electron microscopy of the organisms stained with ruthenium red. the opaque-type colonies of the strains had capsular materials. on the other hand, three t ...	1985	2578434
intestinal infection with mycobacterium avium in acquired immune deficiency syndrome (aids). histological and clinical comparison with whipple's disease.	at endoscopy, a 30-year-old man with acquired immune deficiency syndrome (aids), kaposi's sarcoma, diarrhea, and unexplained malabsorption showed erythematous macular duodenal lesions consistent with whipple's disease by histology and electron microscopy. symptoms did not respond to tetracycline. subsequent cultures revealed systemic mycobacterium avium (m. avium) infection. tissue from this patient, from patients with whipple's disease and from a macaque with m. avium were compared. all contain ...	1985	2580679
catalase enhances damage to dna by bleomycin-iron(ii): the role of hydroxyl radicals.	bleomycin degrades dna under aerobic conditions when a ferrous salt is added. this reaction is enhanced by catalase and certain hydroxyl radical scavengers but inhibited by the addition of hydrogen peroxide. a ferricbleomycin complex is, however, stimulated by addition of hydrogen peroxide. these findings suggest that catalase removes hydrogen peroxide and in so doing prevents loss of ferrous ions and formation of hydroxyl radicals (oh.) by a fenton-type reaction. it further suggests that oh. ra ...	1985	2409975
a study on the pharmacokinetics in mouse of adenine-9-beta-d-arabinofuranoside 5-monophosphate conjugated with lactosaminated albumin.	in plasma of mice injected with adenine-9-beta-d-arabinofuranoside monophosphate (ara-amp) coupled to human lactosaminated serum albumin (l-hsa) some of the ara-amp molecules are enzymatically released, whereas others remain linked to l-hsa. evidence has been obtained that ara-amp is not deaminated when it is conjugated to l-hsa, in contrast to the free drug which is rapidly metabolized to its hypoxanthine derivative.	1985	2412878
antibiotic therapy for vibrio vulnificus infection.		1985	3968842
vibrio vulnificus revisited.		1985	3969801
purification and characterization of an extracellular cytolysin produced by vibrio vulnificus.	an extracellular cytolytic toxin produced by the halophilic bacterium vibrio vulnificus was isolated free of detectable contamination with medium constituents and other bacterial products by sequential ammonium sulfate precipitation, gel filtration with sephadex g-75, hydrophobic interaction chromatography with phenyl-sepharose cl-4b, and isoelectric focusing in an ethylene glycol density gradient. the cytolysin is a heat-labile, hydrophobic protein that is inhibited by large amounts of choleste ...	1985	3980095
metastatic nontraumatic clostridium septicum osteomyelitis.	nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated.	1985	3980945
molecular mechanisms of olfactory reception. vi. kinetic characteristics of camphor interaction with binding sites of rat olfactory epithelium.	camphor binding to a possible receptor of rat olfactory epithelium has been studied within the ligand concentration range 10(-11)-10(-6) m. at these concentrations camphor is bound by a set of receptors. they are distinguished by both the affinity to the ligand (k1 = 5 x 10(-10) m, k2 = 3.5 x 10(-8) m, k3 approximately equal to 10(-6) m) and their amount in the epithelium. the differences in the affinities are due to different values of the association rate constant of camphor (k1), which varies ...	1985	3986222
vibrio vulnificus. man and the sea.	to identify risk factors for vibrio vulnificus infections, we performed a regional case-control study of 19 patients identified by isolates received at a state reference laboratory. interviews with patients or surviving relatives and with three controls for each patient were compared in a matched analysis. patients with v vulnificus wound infection were more likely than controls to have sustained a puncture wound while handling fresh seafood or to have been exposed to salt water. more patients w ...	1985	3989959
[persistent chlamydia infection in cell culture].		1985	3993192
vibrio vulnificus and patients with liver disease.		1985	3996803
fatal septicaemia due to vibrio vulnificus.		1985	4002991
surgical treatment for infective endocarditis--decision tree for the treatment and introduction of translocation method.	persistent fever during active-phase ie is an indication for surgical treatment in the light of the causative organism and the underlying disease. to counter worsening hemodynamics, surgery may be the only feasible way. surgery is performed when the patient does not respond to vasodilators and he shows an fs of less than 25%. the introduction of translocation provides a solution for the treatment of active ie. lesions, vegetations more than 5 mm in diameter, periannular abscess, and mycotic aneu ...	1985	4021068
topographic organization of projections from the amygdala to the hypothalamus of the rat.	afferent fibers from the amygdala to subdivisions of lateral, ventromedial and dorsomedial hypothalamic nuclei were investigated in rat by retrograde transport of horseradish peroxidase. small (intranuclear size) peroxidase deposits were placed in hypothalamic nuclei by iontophoresis of a tracer solution containing poly-l-alpha-ornithine which greatly limited diffusion. the medial, central and amygdalo-hippocampal nuclei of the amygdala were found to be the major donors of amygdaloid afferent fi ...	1985	4022458
detection of reticulo-endothelial blockade with low-dose test agent.	blood clearance and organ extraction of a low-dose reticulo-endothelial test agent, technetium labelled tin colloid (ttc), was measured in groups of rabbits pretreated with reticulo-endothelial blocking agents. electron microscopy and ultrastructure analysis confirmed that kupffer cells extracted ttc. pretreatment with silica caused reduced kupffer cell uptake and spillover of ttc into the spleen. pretreatment with sheep red cells caused reduced kupffer cell uptake and reduced splenic uptake but ...	1985	4027180
vibrio vulnificus resists phagocytosis in the absence of serum opsonins.	invasive disease caused by vibrio vulnificus may result partially from resistance to phagocytic host defense mechanisms. the present studies show that v. vulnificus resists phagocytosis by murine peritoneal macrophages in the absence of serum opsonins and extracellular bacterial products, apparently through the anti-phagocytic properties of the bacterial surface.	1985	4030101
the incidence of rhodococcus (corynebacterium) equi in domestic animals and soil.		1985	4032938
the early revascularization of membranous bone.	the experimental finding that membranous onlay bone grafts maintain volume and viability to a greater extent than do endochondral grafts may be related to the more rapid vascularization of membranous bone. microangiographic techniques were used to study the rates of vascularization of membranous and endochondral bone grafts in adult white new zealand rabbits at 1, 3, 7, 14, and 21 days after bone grafting. vascularization patterns were quantified microscopically using a modified point-counting t ...	1985	4034769
vibrio vulnificus septicemia.	a 33-year-old japanese male, who had a three year history of biopsy-proved liver cirrhosis, was admitted to the hospital on june, 24, 1983 with a sudden onset of fever (38.6 degrees c), chills, generalized pain, nausea, anorexia, weakness, and eruption over the entire body. the patient went into shock and died about 7 hours after admission. blood cultures before death were positive for v. vulnificus. postmortem microscopic examination revealed "necrotizing vasculitis" in the small and large inte ...	1985	4036604
the effects of hypolipidaemic benzodiazepines on serum lipoproteins in hyperlipidaemic rats.		1985	4037206
vibrio vulnificus in sea water: a possible cause of acute diarrhea in man?		1985	4038051
inhibition of lipases by proteins. a kinetic study with dicaprin monolayers.	we report further investigations on protein inhibition of pancreatic and microbial lipases carried out with the monolayer technique. when beta-lactoglobulin a, melittin, serum albumin, myoglobin, and a protein inhibiting lipase from soybean were preincubated with a dicaprin film at a surface pressure of 35 dynes/cm, no activity was detected with horse pancreatic or rhizopus delemar lipases. by contrast, rhizopus arrhizus and geotrichum candidum lipase activities were not impaired under the same ...	1985	4038705
local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males.	the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ...	1985	4040921
aryl hydrocarbon hydroxylase induction in adult rat hepatocytes in primary culture by several chlorinated aromatic hydrocarbons including 2,3,7,8-tetrachlorodibenzo-p-dioxin.	the induction of aryl hydrocarbon hydroxylase (ahh) by aroclor 1254, 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) and other polychlorinated organic compounds was examined in primary cultures of adult rat hepatocytes isolated by a collagenase perfusion technique. following exposure of fresh hepatocyte cultures to 1 microgram/ml aroclor 1254, ahh induction was undetected for 48 hr and then increased dramatically up to 96 hr. cultures maintained in control medium for either 24, 48, or 72 hr prior to ...	1985	4043593