Publications

structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.	physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...	1986	2946570
ultrastructural changes in the sarcoplasmic reticulum in acute myocardial ischemia.	we investigated the morphologic changes in the sarcoplasmic reticulum (sr) in acute myocardial ischemia, induced by occlusion of the coronary artery in the canine heart, by freeze-fracture of sr in situ and in vitro, and they were compared to the alterations in ca++-stimulated atpase activity and protein composition of the isolated sr. both sr in situ and the isolated sr exhibited typical intramembranous particles with diameters of 70 to 90 a in freeze-fracture replicas. the intramembranous part ...	1986	2948034
synthesis and quantitative structure-activity relationships of antiallergic 2-hydroxy-n-1h-tetrazol-5-ylbenzamides and n-(2-hydroxyphenyl)-1h-tetrazole-5-carboxamides.	the synthesis and antiallergic activity of a series of 2-hydroxy-n-1h-tetrazol-5-ylbenzamides and isomeric n-(2-hydroxyphenyl)-1h-tetrazole-5-carboxamides is described. a relationship between structure and intravenous antiallergic activity in the rat passive cutaneous anaphylaxis (pca) test has been established using a hansch/free-wilson model and used to direct studies toward potent derivatives. the contribution of physicochemical properties to activity is discussed. one member of this series, ...	1986	2870188
report of a wound infection caused by vibrio parahaemolyticus and vibrio vulnificus.	the present case describes a foot wound caused by a clam shell from which both vibrio parahaemolyticus and vibrio vulnificus were recovered. although extraintestinal infections associated with vibrio parahaemolyticus have been reported previously, the simultaneous isolation of two marine vibrios from our case suggests that these organisms may coexist in mixed infections from a common source.	1986	2875824
effects of somatostatin and sms 201-995 on carbohydrate metabolism in normal man.	growth hormone (gh) is important in diabetes in view of its anti-insulin actions and its relation to the long-term complications of the disease. the suppression of gh secretion in diabetics has theoretical and possible therapeutic interest. native somatostatin has multiple actions, including inhibition of the secretion of insulin, glucagon, thyroid-stimulating hormone (tsh), and various gut hormones. it also has inhibitory effects on gastrointestinal motility, exocrine secretion, nutrient absorp ...	1986	2876501
[human gingival pigmentation due to electrochemical degradation. immunological study of biopsies using monoclonal antibodies].		1986	3464603
second-generation cephalosporins.		1986	3081544
identification of n epsilon-carboxymethyllysine as a degradation product of fructoselysine in glycated protein.	the chemistry of maillard or browning reactions of glycated proteins was studied using the model compound, n alpha-formyl-n epsilon-fructoselysine (ffl), an analog of glycated lysine residues in protein. incubation of ffl (15 mm) at physiological ph and temperature in 0.2 m phosphate buffer resulted in formation of n epsilon-carboxymethyllysine (cml) in about 40% yield after 15 days. cml was formed by oxidative cleavage of ffl between c-2 and c-3 of the carbohydrate chain and erythronic acid (ea ...	1986	3082871
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.	lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...	1986	3085110
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.	lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...	1986	3085110
effect of nifedipine on cerebral high-energy phosphates after cardiac arrest and resuscitation in the rat.	we studied the effect of nifedipine, a calcium entry blocker, on the recovery of cerebral adenosine triphosphate (atp), creatine phosphate (cp), and lactate levels following resuscitation from cardiac arrest. using the cardiac arrest and resuscitation model of de garavilla, babbs, and tacker with an arrest time of eight minutes, 76% of the animals arrested were resuscitated with an average intermittent abdominal compression-cpr time of 3.3 minutes. rats were assigned randomly to the following gr ...	1986	3085559
evidence that some membrane ligands modulate the plasmalemma fluidity of endothelial cells in culture.	the effect of some membrane ligands on the plasmalemmal fluidity of endothelial cells from bovine aorta in culture was investigated. the ligands used were: cationic ferritin (pi 8.5), soybean agglutinin, concanavalin a, wheat germ agglutinin, as well as glutaraldehyde at different concentrations. the fluidity probe employed was 1,6-diphenyl-1, 3, 5-hexatriene (dph) and the parameter determined to quantify the fluidity was fluorescence steady-state anisotropy. the optimum time interval required b ...	1986	3088620
nasopharyngeal carriage of neisseria meningitidis in the contacts of index cases of meningococcal meningitis.		1986	3091676
[a saved case of vibrio vulnificus infection with shock, dic and necrotizing fasciitis].		1986	3097209
protamine sulfate-induced enzyme secretion from rabbit neutrophils.	protamine sulfate induces enzyme secretion from rabbit neutrophils. enzyme secretion is mainly due to exocytosis but, depending on the experimental conditions, a small amount of cytolysis may occur. as compared with stimulation of neutrophil functions by other activators, protamine sulfate-induced enzyme release by exocytosis is a relatively slow process and is not accompanied by a marked activation of the metabolic burst. for optimal exocytosis, extracellular ca2+ is required, but there is stil ...	1986	3098680
escherichia coli as cause of ecthyma gangrenosum.		1986	3511469
feasibility of same-day identification of members of the family vibrionaceae by the api 20e system.	sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ...	1986	3517061
immunocytochemical localization of cathepsin b in rat kidney. ii. electron microscopic study using the protein a-gold technique.	thin sections of lowicryl k4m-embedded materials were labeled with protein a-gold complex. gold particles representing the antigen sites for cathepsin b were exclusively confined to lysosomes of each segment of the nephron. the heaviest labeling was noted in the lysosomes of the s1 segment of the proximal tubules. labeling intensity varied considerably with the individual lysosomes. lysosomes of the other tubular segments, such as the s2 and s3 segments of the proximal tubules, distal convoluted ...	1986	3519753
production of extracellular enzymes and cytotoxicity by vibrio vulnificus.	thirty-three strains of vibrio vulnificus of clinical and environmental origin were examined for production of 12 extracellular enzymes of potential importance to the virulence of this bacterium. strains of vibrio vulnificus were consistent in their production of protease, mucinase, lipase, chondroitinase, hyaluronidase, dnase, sulfatase, and hemolysin. no differences between clinical and environmental isolates were noted. although none of the enzymes appeared to correlate with the ability of th ...	1986	3522090
chitinase determinants of vibrio vulnificus: gene cloning and applications of a chitinase probe.	to initiate study of the genetic control of chitinolytic activity in vibrios, the chitobiase gene was isolated by cloning chromosomal dna prepared from vibrio vulnificus. chimeric plasmids were constructed from sau3a i partial digests of chromosomal dna by ligating 5 to 15-kilobase fragments into the bamhi site, i.e., in the tcr gene, of pbr322 (amr tcr). the resulting plasmids were transformed into escherichia coli dh1. chitobiase activity of the insert-bearing clones was detected by using a ch ...	1986	3524459
[a case of sepsis, shock, disseminated intravascular coagulation, and necrotizing fasciitis caused by vibrio vulnificus treated with early debridement].		1986	3559356
[mast cell-affinity lymphocytes as indicator cells of the forms of hemoblastoses].	the formation of mastolymphocyte rosettes was used to determine that in chronic lymphocytic leukemia and bovine leukemia, unlike healthy people and animals as well as patients with solid tumours of different localization and hodgkin's disease, a large amount of cells appear among peripheral blood lymphocytes having membrane affinity for mast cells. this may be used as an additional criterion in laboratory diagnosis of these diseases.	1986	3485042
experience with isoxicam and catabolin.	1 synovial cells produce a protein factor, catabolin, that induces chondrocytes to resorb their own matrix with the release of glycosaminoglycan (gag). 2 studies were performed to determine the effect of isoxicam, a new nsaid, on gag production in a catabolin system utilizing tissue culture of bovine nasal septum in a medium containing pooled synovial tissue from young pigs. 3 using fresh medium containing no synovial tissue, the release of gag by inherent cartilage breakdown was 49.7% after 8 d ...	1986	3497659
vibrio vulnificus septicemia: report of four cases.		1986	3564544
plasmid carriage in vibrio vulnificus and other lactose-fermenting marine vibrios.	a total of 42 clinical and environmental isolates of vibrio vulnificus were examined for plasmid carriage. of these, only five (12%) harbored plasmids, which were of various molecular weights. in contrast, 20 of 32 (62.5%) unidentified lactose-fermenting vibrio spp. were found to possess plasmids with masses of 2.1 to 150 megadaltons. in these isolates, multiple plasmids were common, with an average of 2.25 plasmids per plasmid-containing strain. attempts to demonstrate a correlation with the pl ...	1986	3729404
purification and initial characterization of the 71-kilodalton rat heat-shock protein and its cognate as fatty acid binding proteins.	the major rat heat-shock (stress) protein and its cognate were purified to electrophoretic homogeneity from livers of heat-shocked rats. both proteins exhibited similar behavior on a variety of column chromatography matrices but were separable by preparative isoelectric focusing under nondenaturing conditions by virtue of a 0.2 ph unit difference in isoelectric point. both purified proteins had similar physical properties, suggesting the possibility that they may have similar biological function ...	1986	3730359
potentiation of hemorrhage-evoked catecholamine release by prior blood loss in cats.	the effect of prior blood loss on the plasma catecholamine response to acute hemorrhage (h) was assessed in alpha-chloralose-urethane anesthetized cats. animals sustained an initial h period of 0 (samples only), 10, or 20% h total blood volume. ninety minutes after reinfusion of the shed blood, all animals sustained a rapid 20% h. the catecholamine and arterial pressure responses to this second 20% h were assessed every 2 min for 20-min duration. plasma norepinephrine increased modestly in the 0 ...	1986	3510559
hemorrhagic bullae associated with vibrio vulnificus septicemia. report of two cases.	bullous lesions associated with vibrio vulnificus infection developed in two patients, both of whom had hepatic cirrhosis. one patient had a recent history of ingestion of raw oysters, while the other patient had recently exposed skin lacerations to sea water. both patients died within 24 hours of hospitalization, in spite of antibiotic treatment. vibrio vulnificus was isolated from blood and bullae in both patients. histologic examination of skin biopsy specimens revealed epidermal/dermal separ ...	1986	3524472
effect of alterations in lipid packing order by hydrophobic solutes on the association state of protein assemblies in model membranes.	the effects of two hydrophobic solutes which perturb lipid packing order, permethrin and allethrin, on the aggregated state of a lipid membrane-incorporated protein, bacteriorhodopsin (br), have been determined by resonance energy transfer measurements. as temperature is increased from well below the main gel-fluid phase transition temperature (tc) of the lipid, patches of aggregated br dissociate into monomers, a few degrees below the tc (m.p. heyn, a. blume, m. rehorek and n.a. dencher, bioche ...	1986	3754778
stabilization of intracellular recordings in the cat spinal cord using high-frequency ventilation.	stabilization of intracellular recordings by a substantial reduction of respiratory motion may be accomplished by the use of high-frequency ventilation (600-800 breaths/min). the technique is a simple one and enables maintenance of relatively constant blood gas tensions. although the improved stability was demonstrated in experiments performed on alpha motoneurones in the thoracic spinal cord, it is anticipated that a reduced respiratory movement should benefit recordings in other spinal and sup ...	1986	3756629
a reappraisal of the complement fixation test using soluble mycobacterium avium antigen for the detection of m. paratuberculosis infection in cattle.	serums from 263 cattle suspected of having paratuberculosis on the basis of clinical signs, were tested for antibodies to mycobacterium paratuberculosis with a complement fixation test (cft) employing a heat extracted, soluble m. avium antigen. microscopic examination confirmed that 172 (65.4%) clinically affected animals had paratuberculosis, the remainder being disease-free. the specificity and sensitivity of the cft was 92.3% and 74.4% respectively. phenol treatment of serums before testing w ...	1986	3753338
rhamnetin-3-o-neohesperidoside, a new flavonoid from the leaves of derris trifoliata.		1986	3509960
[a case of vibrio vulnificus infection].		1986	3795561
[changes in the characteristics of glucocorticoid hormone binding in human peripheral blood lymphocytes during myocardial infarct].	competitive binding was used to determine the number of binding sites to glucocorticoid hormones in peripheral blood leukocytes of normal subjects, patients with unstable angina pectoris and patients with myocardial infarction. a sharp (2-3-fold) increase in the number of binding sites was observed in patients with myocardial infarction during the first 24 hours after the onset of the disease.	1986	3801148
comparison of histones in normal and xeroderma pigmentosum lymphoblastoid cells.	histones from normal human and xeroderma pigmentosum, complementation group a (xpa), lymphoblastoid cells were compared both quantitatively, qualitatively and for binding affinity for dna. electrophoretic examination of the histones showed that all five major histone species were present in both cell groups and that there were no quantitative differences between normal and xpa histones. binding affinity to [3h] mammalian dna of the histones was determined. no significant differences were observe ...	1986	3791423
sensitive analytical method for serum primidone and its active metabolites for single-dose pharmacokinetic analysis in human subjects.		1986	3769107
participation of iron on the growth inhibition of pathogenic strains of mycobacterium avium and m. paratuberculosis in serum.	serum, which is low in freely-available iron, was bacteriostatic for m. avium and m. paratuberculosis irrespective of the ability of these organisms to produce the iron-chelators mycobactin and exochelin for iron acquisition. bacteriostasis was overcome when the serum was supplemented with exochelin or mycobactin, but only if the mycobacteria had previously adapted to growth in a low iron environment. the results imply that for studies on mycobacterial pathogenicity the test cultures should be g ...	1986	3788345
isolation and characterization of a mucin-degrading strain of peptostreptococcus from rat intestinal tract.	a mucin-degrading microorganism was isolated from the intestinal tract by serial sectioning from the serosal side of the caecum wall from a conventional rat. the ability of degrading the intestinal water-soluble mucin was present both in vivo after monocontamination of germ-free rats and in vitro, when adding the microbe to mucin medium. the morphology, gram-positive cocci single or in short chains and the very weak biochemical activities allow us to place this strain in the species peptostrepto ...	1986	3788570
bioluminescence in a strain of the human pathogenic bacterium vibrio vulnificus.	we report the existence of a bioluminescent strain of the human pathogen vibrio vulnificus. the isolate was obtained from blood following a fatal wound infection and thus represents the first description of an infection caused by a luminescent bacterium.	1986	3789716
surgical management of peritoneal dialysis catheters in children: five-year experience with 1,800 patient-month follow-up.	currently at our institution more than 90% of the children with end-stage renal disease are managed with continuous ambulatory peritoneal dialysis (capd) in preference to hemodialysis until a successful transplant is accomplished. recent refinements in capd catheters and dialysis techniques have greatly added to the many medical, psychological, and economic advantages of capd compared with chronic hemodialysis. ninety-three patients less than 21 years of age underwent insertion of 167 peritoneal ...	1986	3794985
comparison of particle-associated progesterone and oxytocin in the ovine corpus luteum.	the subcellular distribution of progesterone and oxytocin within the ovine corpus luteum was investigated using differential and density gradient centrifugation. progesterone and oxytocin were associated with particles which sedimented to a density of 1.049-1.054 g/ml and 1.054-1.061 g/ml respectively. particle-associated progesterone did not, however, display physical or biochemical characteristics consistent with its storage within secretory granules. when particle-associated progesterone was ...	1986	3944530
rapid serological identification of vibrio vulnificus by anti-h coagglutination.	staphylococcus aureus cowan 1 cells were armed with anti-flagellar (anti-h) antibody produced in rabbits immunized with flagellar core protein prepared from vibrio vulnificus. this reagent was assessed by coagglutination for its capacity to agglutinate and identify v. vulnificus. a species-specific h antigen is expressed in the core proteins of the polar flagella of v. vulnificus. of 435 v. vulnificus isolates identified bacteriologically, 432 (99.3%) were agglutinated in the slide test within 2 ...	1986	3789720
esculin hydrolysis by vibrio vulnificus.	a clinical isolate of vibrio vulnificus was found to hydrolyze esculin when tested on bile-esculin-azide agar during the initial characterization of the strain. reports in the literature of esculin hydrolysis by v. vulnificus are conflicting. we tested herein 52 strains of v. vulnificus from clinical and environmental sources for the ability to hydrolyze esculin. seventy-eight percent of the strains hydrolyzed esculin on bile-esculin-azide agar, whereas all strains of v. vulnificus tested were p ...	1986	3943291
gastroenteritis in patients with stool isolates of vibrio vulnificus.	vibrio vulnificus, a marine vibrio associated with severe extraintestinal infections, has not been previously implicated as a cause of infectious diarrhea. three patients were identified with diarrheal illness from whom this organism was the sole pathogen recovered from cultured stool specimens. all three had eaten raw oysters within one week of becoming ill. these patients were all taking medication that reduces gastric acidity, two were heavy drinkers of alcohol, and one had unrecognized colon ...	1986	3946453
detection of anti-vibrio vulnificus cytolysin antibodies in sera from mice and a human surviving v. vulnificus disease.	an enzyme-linked immunosorbent assay and a cytolysin neutralization assay were used detect anti-vibrio vulnificus cytolysin antibodies in sera from mice and a human that survived v. vulnificus disease. the detection of antibodies against the cytolysin indicated that the cytolysin is produced in vivo, and this observation is consistent with the hypothesis that the cytolysin is involved in the pathogenesis of v. vulnificus disease.	1986	3949387
emergence of bactericidal and opsonizing antibody to vibrio vulnificus following bacterial infection.	virulent isolates of vibrio vulnificus resist the bactericidal and opsonizing effects of normal human serum, in contrast to environmental isolates, which are highly serum susceptible. immune responses to bacteremic v. vulnificus infections in human subjects have not been characterized. serum from a patient who survived sepsis caused by v. vulnificus had substantial bactericidal and opsonizing immunoglobulin g (igg) for his own bloodstream isolate. killing was mediated by the classical complement ...	1986	3958138
calmodulin gene expression during sea urchin development: persistence of a prevalent maternal protein.	calmodulin gene expression during embryogenesis of the sea urchin strongylocentrotus purpuratus was investigated. several identical bacteriophages containing a cdna insert encoding sea urchin calmodulin (cm-1) were identified by screening a lambda gt10 library of s. purpuratus gastrula-stage cdnas with a chicken calmodulin cdna sequence. a 1.2-kb cdna fragment from cm-1 was subcloned into puc-8 to give plasmid pcal-8. pcal-8 contains a single open reading frame encoding 79 amino acids, a termina ...	1986	3753945
studies on cardiac distribution and function of neuropeptide y.	high concentrations of a novel peptide, neuropeptide y, have been demonstrated in the guinea-pig and canine heart and in the latter, a particularly high concentration was found in the region of the coronary vasculature (126 +/- 31 pmol g-1). intra-arterial infusion of neuropeptide y for 30 s into the coronary artery of the intact, innervated dog heart resulted in a rapid and short-lasting reduction of blood flow from 38 +/- 4 to 31 +/- 3 ml min-1 (p less than 0.05) to resume control level, 39 +/ ...	1986	3754377
breath hydrogen concentration and small intestinal malabsorption in calves.	breath hydrogen concentrations were measured to assess intestinal carbohydrate malabsorption in preruminating calves. oral administration of 1.25 g of lactulose (a nonabsorbable carbohydrate)/kg to calves produced breath hydrogen concentrations significantly (p less than 0.001) higher than values determined after calves were fed milk and before the treatment was given. this indicates that, in the calf, fermentation of nonabsorbed carbohydrates results in increased breath hydrogen values. to indu ...	1986	3767107
inland presentation of vibrio vulnificus primary septicemia and necrotizing fasciitis.		1986	3953073
anal submucosal injection: a new route for drug administration in pelvic malignancies. part i. experimental study of misonidazole distribution in serum and tissues, with special reference to urinary bladder. preliminary report.	the anal and oral administration routes were compared in 30 rats to study the distribution of misonidazole, a radiation sensitizer, in the serum and tissues with special reference to the urinary bladder. 14c-labelled misonidazole was administered in a dose of 0.2 ml water/100 gm body weight containing 1 mu ci misonidazole. the dose was given orally by stomach tube in 15 rats, and was injected in the submucosa of the anal canal in another 15. animals were then killed after 15, 30, 60, or 120 minu ...	1986	3957602
composition of major outer membrane proteins of vibrio vulnificus isolates: effect of different growth media and iron deficiency.	the outer membrane proteins of vibrio vulnificus including isolates from humans, seawater and an asari clam were examined by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis. a major outer membrane protein with an apparent molecular weight of 48,000 (48k protein) was common to all the strains grown in 3% nacl-nutrient broth; however this 48k protein was not produced in any of the strains grown in chemically defined medium. other major outer membrane proteins with molecular weights ...	1986	3724558
virulence of vibrio vulnificus strains from marine environments.	vibrio vulnificus strains isolated from geographically diverse marine sources were compared with clinical isolates for phenotype and in vitro and in vivo production of virulence factors. there were no differences between environmental and clinical strains on the basis of biochemical characteristics or antimicrobial susceptibility patterns. cytolysin and cytotoxin titers produced by environmental strains were generally comparable to those of clinical strains. of 29 environmental isolates tested, ...	1986	3729387
preparation, isolation and characterization of mouse ige monoclonal antibodies against trichosanthin protein.		1986	3739501
evaluation of the hereditary syrian hamster cardiomyopathy by 31p nuclear magnetic resonance spectroscopy: improvement after acute verapamil therapy.	the relation between metabolic and functional derangement in various cardiomyopathies has not been well characterized. this information was specifically sought in a spontaneous cardiomyopathic model. metabolic and hemodynamic parameters were obtained in glucose-perfused beating hearts of 180-200-day-old cardiomyopathic syrian hamsters and age-matched healthy animals. this period in the cardiomyopathic hamster lifetime is intermediary between the necrotic phase and the appearance of heart failure ...	1986	3815756
processing-independent in vitro translocation of cytochrome p-450(scc) precursor across mitochondrial membranes.	in the presence of a membrane-permeable metal chelator, bovine adrenal cortex mitochondria imported p-450(scc) precursor without processing of the amino-terminal extension peptide. the imported precursor was bound to the matrix side surface of the inner membrane. when the inhibition due to the metal chelator was removed, the imported precursor was processed to the mature form. unprocessed precursor was also detected in mitochondria when the import reaction was carried out at relatively low tempe ...	1986	3818579
similarity principles and intrinsic geometries: contrasting approaches to interspecies scaling.	we criticize standard allometric approaches on the grounds that they emphasize scaling to one variable at a time, whereas chemically reactive hydrodynamic systems involved in pharmacokinetic phenomena are of higher dimension. we show that attempts based on mechanical similitude to set a dosage that would be equivalent across species (for example, from mouse to humans) lead to ambiguous results. another failing of standard allometry may be its incapability to accommodate the neoteny of homo sapie ...	1986	3820095
degenerative lesions of a coronary chemoreceptor and nearby neural elements in the hearts of victims of sudden death.	a coronary chemoreceptor and its neighboring nerves and ganglia were studied by serial sections of tissue taken from the hearts of sixteen victims of sudden unexpected death. either focal or extensive inflammatory destruction was present in the chemoreceptors of 13 of the 16 hearts, and similar abnormalities of local nerves and ganglia were present in all 16 hearts. since an identical coronary chemoreceptor in the dog has previously been shown to be the site of origin of a powerful reflex with m ...	1986	3837961
isolation and characterization of neuropeptide y from porcine intestine.	the isolation and primary structure of intestinal neuropeptide y (npy) is described. the peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for npy. the amino acid sequence of this peptide is: tyr-pro-ser-lys-pro-asp-asn-pro-gly-glu-asp-ala-pro-ala-glu-asp-leu-ala- arg-tyr-tyr- ser-ala-leu-arg-his-tyr-ile-asn-leu-ile-thr-arg-gln-arg-tyr-nh2. this the structure of intestinal npy is identical to the npy of brain origin.	1985	3838090
[an experimental study on adhesion blocking in simultaneous injury of tendon and nerve].	the formation of adhesions between simultaneously injured tendon and nerve with and without adhesion blocking materials was studied by using a rabbit model. a collagen membrane, produced from adult bovine corium and with a negative electric charge induced by chemical modification, was used as the blocking material. microangiography and histological studies were performed to investigate the vascular supply to the adhesions. the following results were obtained. blood vessel proliferation was obser ...	1985	3831164
[effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats].		1985	3835674
the prostaglandin h synthase-dependent activation of 2-aminofluorene to products mutagenic to s. typhimurium strains ta98 and ta98nr.		1985	3925332
pressure- and temperature-dependent adhesion of pseudomonas aeruginosa to hep-2 cells.	pseudomonas aeruginosa bacteria adhere to human epitheloid (hep-2) cells in culture. under normal atmospheric conditions (1 ata), this adhesion increased significantly when the temperature rose from 22 to 37 degrees c. under hyperbaric atmosphere (= air, 7 ata) conditions, a similar, significant enhancement of bacterial adhesion to the cells was noted when the temperature rose. if the temperature was kept stable at 22 or 37 degrees c and the pressure was increased from 1 to 7 ata, a pressure-ind ...	1985	3925713
synthesis of dihomoprostaglandins from adrenic acid (7,10,13,16-docosatetraenoic acid) by human endothelial cells.	human umbilical vein endothelial cells were found to contain adrenic acid (22:4) in their cellular lipids. since this fatty acid may be metabolized by cyclooxygenase in the kidney, the metabolism of adrenic acid was studied in endothelial cell cultures. [14c]adrenic acid was metabolized to several more polar metabolites. two of these metabolites co-migrated on hplc with 1 alpha,1 beta-dihomo-8-ketoprostaglandin f1 alpha (the metabolite of 1 alpha, 1 beta-dihomoprostaglandin i2) and 1 alpha,1 bet ...	1985	3931686
location of polar substituents and fatty acyl chains on lipid a precursors from a 3-deoxy-d-manno-octulosonic acid-deficient mutant of salmonella typhimurium. studies by 1h, 13c, and 31p nuclear magnetic resonance.	eight anionic disaccharide precursors of lipid a accumulate at 42 degrees c in 3-deoxy-d-manno-octulosonic acid-deficient temperature-sensitive mutants of salmonella typhimurium. these compounds comprise a series of lipids based on the minimal structure, o-[2-amino-2-deoxy-n2,o3-bis(3-hydroxytetradecanoyl)-beta-d-glucopyranos yl] -(1----6)-2-amino-2-deoxy-n2, o3-bis(3-hydroxytetradecanoyl)-alpha-d-glucopyranose 1,4'- bisphosphate (designated lipid iva) that differ from each other by the presence ...	1985	3905805
location of polar substituents and fatty acyl chains on lipid a precursors from a 3-deoxy-d-manno-octulosonic acid-deficient mutant of salmonella typhimurium. studies by 1h, 13c, and 31p nuclear magnetic resonance.	eight anionic disaccharide precursors of lipid a accumulate at 42 degrees c in 3-deoxy-d-manno-octulosonic acid-deficient temperature-sensitive mutants of salmonella typhimurium. these compounds comprise a series of lipids based on the minimal structure, o-[2-amino-2-deoxy-n2,o3-bis(3-hydroxytetradecanoyl)-beta-d-glucopyranos yl] -(1----6)-2-amino-2-deoxy-n2, o3-bis(3-hydroxytetradecanoyl)-alpha-d-glucopyranose 1,4'- bisphosphate (designated lipid iva) that differ from each other by the presence ...	1985	3905805
mechanism of sbcb-suppression of the recbc-deficiency in postreplication repair in uv-irradiated escherichia coli k-12.	the mechanism by which an sbcb mutation suppresses the deficiency in postreplication repair shown by recb recc mutants of escherichia coli was studied. the presence of an sbcb mutation in uvra recb recc cells increased their resistance to uv radiation. this enhanced resistance was not due to a suppression of the minor deficiency in the repair of dna daughter-strand gaps or to an inhibition of the production of dna double-strand breaks in uv-irradiated uvra recb recc cells; rather, the presence o ...	1985	3911022
[effect of r plasmids on the growth characteristics of shigella sonnei and escherichia coli].	revealing of growth characteristics in plasmid and plasmid-free strains was studied with the use of two different hosts: e. coli 15-3 and sh. sonnei 11-941 containing conjugative r plasmids differing in the set of the resistance markers. it was shown that the r plasmids had no noticeable effect on the period of the lag phase and the time of the microbial cell generation. it was also shown that the number of the viable cells in separate cultures of the plasmid-free strain of sh. sonnei 11-941 and ...	1985	3911872
rapid microimmunodiffusion method with species-specific antiserum raised to purified antigen for identification of vibrio vulnificus.	an antigen common to vibrio vulnificus strains, designated vva, was purified by ammonium sulfate precipitation, gel filtration, ion-exchange column chromatography, and preparative gel electrophoresis. the molecular weight of vva was 64,000 when estimated by gel filtration and 40,000 when measured by denaturing polyacrylamide gel electrophoresis. antiserum prepared against purified vva (anti-vva serum) did not agglutinate whole cells of v. vulnificus. therefore, vva was considered a possible inte ...	1985	3918072
human c-myc and n-ras expression during induction of hl-60 cellular differentiation.	the genome of the human hl-60 promyelocytic leukemia cell contains amplified c-myc sequences and the transforming n-ras oncogene. the present study has monitored c-myc and n-ras expression in hl-60 cells during induction of myeloid and monocytic differentiation with dimethyl sulfoxide, hexamethylene bisacetamide, 12-o-tetradecanoylphorbol-13-acetate and 1,25 dihydroxy-vitamin d3. the results demonstrate that induction of hl-60 differentiation is associated with decreases in c-myc rna, while ther ...	1985	3856429
selective uptake of a toxic lipophilic anthracycline derivative by the low-density lipoprotein receptor pathway in cultured fibroblasts.	n-(n-retinoyl)-l-leucyldoxorubicin 14-linoleate (r11-dox), a new lipophilic derivative of doxorubicin, was synthesized and incorporated into low-density lipoprotein (ldl). the drug-ldl complex contained 100-200 drug molecules/ldl particle. when cultured normal human fibroblasts were incubated with 125i-ldl-incorporated drug, there was a perfect correlation between the cellular uptake plus degradation of 125i-ldl and the cellular drug accumulation. the presence of excess native ldl inhibited the ...	1985	3856679
in vitro activity of ci-934, a quinolone carboxylic acid active against gram-positive and -negative bacteria.	ci-934 is a totally synthetic difluorinated quinolinecarboxylic acid with an ethyl-amino-methyl pyrrolidine side chain, which has broad-spectrum antibacterial activity, including particular potency directed against streptococci and staphylococci. the ci-934 mic (micrograms per milliliter) for 90% of the strains tested was 0.4 (range, 0.2 to 0.8) for a group of streptococci (pneumococci, viridans streptococci, streptococcus faecalis, and lancefield groups a, b, and c), 0.2 (0.05 to 0.2) for staph ...	1985	3866513
characterization with monoclonal antibodies of a surface antigen of plasmodium falciparum merozoites.	the merozoite, the extracellular form of the erythrocyte stage of the malarial parasite, invades the erythrocyte and develops intracellularly. cloned hybridoma cell lines secreting monoclonal antibodies directed against the merozoite surface were selected by indirect immunofluorescent assay by using intact isolated merozoites. monoclonal antibodies to a 200,000 m.w. merozoite surface antigen were selected and were used to characterize this protein and its role in erythrocyte invasion. immunoelec ...	1985	3881529
cloning and expression in escherichia coli of vibrio parahaemolyticus thermostable direct hemolysin and thermolabile hemolysin genes.	two hemolysin genes of vibrio parahaemolyticus wp1, a thermostable direct (tsd) hemolysin gene and a thermolabile hemolysin gene, were cloned into the pbr322 vector in escherichia coli k-12 c600. a large amount of the tsd hemolysin produced in e. coli k-12 accumulated in the periplasmic space. the tsd hemolysin gene was localized on a 0.9-kilobase hindiii-bamhi fragment by identifying qualitatively the production of the tsd hemolysin by a reverse passive hemagglutination assay in the osmotic sho ...	1985	3886626
cardioprotective effects of defibrotide in acute myocardial ischemia in the cat.	we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ...	1985	3890260
cardioprotective effects of defibrotide in acute myocardial ischemia in the cat.	we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ...	1985	3890260
the incidence of rhodococcus (corynebacterium) equi in domestic animals and soil.		1985	4032938
the early revascularization of membranous bone.	the experimental finding that membranous onlay bone grafts maintain volume and viability to a greater extent than do endochondral grafts may be related to the more rapid vascularization of membranous bone. microangiographic techniques were used to study the rates of vascularization of membranous and endochondral bone grafts in adult white new zealand rabbits at 1, 3, 7, 14, and 21 days after bone grafting. vascularization patterns were quantified microscopically using a modified point-counting t ...	1985	4034769
purification and characterization of an extracellular cytolysin produced by vibrio vulnificus.	an extracellular cytolytic toxin produced by the halophilic bacterium vibrio vulnificus was isolated free of detectable contamination with medium constituents and other bacterial products by sequential ammonium sulfate precipitation, gel filtration with sephadex g-75, hydrophobic interaction chromatography with phenyl-sepharose cl-4b, and isoelectric focusing in an ethylene glycol density gradient. the cytolysin is a heat-labile, hydrophobic protein that is inhibited by large amounts of choleste ...	1985	3980095
metastatic nontraumatic clostridium septicum osteomyelitis.	nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated.	1985	3980945
molecular mechanisms of olfactory reception. vi. kinetic characteristics of camphor interaction with binding sites of rat olfactory epithelium.	camphor binding to a possible receptor of rat olfactory epithelium has been studied within the ligand concentration range 10(-11)-10(-6) m. at these concentrations camphor is bound by a set of receptors. they are distinguished by both the affinity to the ligand (k1 = 5 x 10(-10) m, k2 = 3.5 x 10(-8) m, k3 approximately equal to 10(-6) m) and their amount in the epithelium. the differences in the affinities are due to different values of the association rate constant of camphor (k1), which varies ...	1985	3986222
vibrio vulnificus. man and the sea.	to identify risk factors for vibrio vulnificus infections, we performed a regional case-control study of 19 patients identified by isolates received at a state reference laboratory. interviews with patients or surviving relatives and with three controls for each patient were compared in a matched analysis. patients with v vulnificus wound infection were more likely than controls to have sustained a puncture wound while handling fresh seafood or to have been exposed to salt water. more patients w ...	1985	3989959
[persistent chlamydia infection in cell culture].		1985	3993192
vibrio vulnificus and patients with liver disease.		1985	3996803
fatal septicaemia due to vibrio vulnificus.		1985	4002991
surgical treatment for infective endocarditis--decision tree for the treatment and introduction of translocation method.	persistent fever during active-phase ie is an indication for surgical treatment in the light of the causative organism and the underlying disease. to counter worsening hemodynamics, surgery may be the only feasible way. surgery is performed when the patient does not respond to vasodilators and he shows an fs of less than 25%. the introduction of translocation provides a solution for the treatment of active ie. lesions, vegetations more than 5 mm in diameter, periannular abscess, and mycotic aneu ...	1985	4021068
inhibition of lipases by proteins. a kinetic study with dicaprin monolayers.	we report further investigations on protein inhibition of pancreatic and microbial lipases carried out with the monolayer technique. when beta-lactoglobulin a, melittin, serum albumin, myoglobin, and a protein inhibiting lipase from soybean were preincubated with a dicaprin film at a surface pressure of 35 dynes/cm, no activity was detected with horse pancreatic or rhizopus delemar lipases. by contrast, rhizopus arrhizus and geotrichum candidum lipase activities were not impaired under the same ...	1985	4038705
local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males.	the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ...	1985	4040921
antiarrhythmic and electrophysiologic actions of clofilium in experimental canine models.	clofilium was studied in three experimental models. in non-ischemic and chronically infarcted canine hearts, clofilium (0.5-2 mg/kg) produced a dose-dependent increase in electrical ventricular fibrillation threshold (vft), but prolonged the effective refractory period (erp) of normal myocardium in only the non-ischemic heart. when chronically infarcted hearts were subjected to programmed electrical stimulation, 1 mg/kg of clofilium inhibited the re-induction of either ventricular tachycardia or ...	1985	4054216
antibiotic therapy for vibrio vulnificus infection.		1985	3968842
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.	sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...	1985	4056003
cloning of the cytotoxin-hemolysin gene of vibrio vulnificus.	genes encoding the cytotoxin-hemolysin of vibrio vulnificus were cloned in escherichia coli by using the lytic cloning vector, lambda 1059. subcloning in plasmid pbr325 resulted in the isolation of a 3.2-kilobase dna fragment containing the cytotoxin gene. by using this fragment as a dna probe, homologous gene sequences were detected in all 54 v. vulnificus strains studied; homologous sequences were present in none of 96 isolates from 29 other bacterial species.	1985	4066036
case records of the massachusetts general hospital. weekly clinicopathological exercises. case 50-1985. a 41-year-old man with diffuse tracheal narrowing.		1985	4069162
some properties of vibrio vulnificus hemolysin.	some properties of hemolysin produced by vibrio vulnificus were investigated. the hemolysin was heat labile, and the hemolytic activity was inhibited by adding cholesterol or divalent cations. cholesterol inhibited the temperature-independent hemolysin-binding step, suggesting that cholesterol made up the binding site of the cell membrane, whereas the divalent cations inhibited the temperature-dependent membrane-degradation step. however, the v. vulnificus hemolysin was stable to oxygen and sulf ...	1985	4088099
uptake and clearance of vibrio vulnificus from gulf coast oysters (crassostrea virginica).	oysters collected in late winter, when they were free of vibrio vulnificus, were exposed in the organism in the laboratory. the oysters effectively concentrated the bacteria from seawater, but when the inoculum was removed, the bacteria were rapidly cleared from the oyster tissues. these results suggest that v. vulnificus may be found in oysters as a result of filtration of the bacteria from seawater rather than active multiplication of the bacteria in the oysters.	1985	4091570
vibrio vulnificus revisited.		1985	3969801
topographic organization of projections from the amygdala to the hypothalamus of the rat.	afferent fibers from the amygdala to subdivisions of lateral, ventromedial and dorsomedial hypothalamic nuclei were investigated in rat by retrograde transport of horseradish peroxidase. small (intranuclear size) peroxidase deposits were placed in hypothalamic nuclei by iontophoresis of a tracer solution containing poly-l-alpha-ornithine which greatly limited diffusion. the medial, central and amygdalo-hippocampal nuclei of the amygdala were found to be the major donors of amygdaloid afferent fi ...	1985	4022458
detection of reticulo-endothelial blockade with low-dose test agent.	blood clearance and organ extraction of a low-dose reticulo-endothelial test agent, technetium labelled tin colloid (ttc), was measured in groups of rabbits pretreated with reticulo-endothelial blocking agents. electron microscopy and ultrastructure analysis confirmed that kupffer cells extracted ttc. pretreatment with silica caused reduced kupffer cell uptake and spillover of ttc into the spleen. pretreatment with sheep red cells caused reduced kupffer cell uptake and reduced splenic uptake but ...	1985	4027180
demonstration of a common antigen in sonicated cells for identification of vibrio vulnificus serotypes.	ouchterlony immunodiffusion of sonicated vibrio vulnificus cells illustrated a single major precipitation line with antiserum prepared from whole cells of the same species. antigenic analysis by two-dimensional immunoelectrophoresis verified the presence of a single dominant precipitation line. tandem two-dimensional immunoelectrophoretic analyses of v. vulnificus antigens from various strains revealed one fused precipitation line of identity. no fused precipitation lines were seen with other vi ...	1985	3918076
spontaneous vibrio vulnificus peritonitis and primary sepsis in two patients with alcoholic cirrhosis.	two patients with alcoholic cirrhosis were seen on two separate occasions for fever, swollen legs, petechial hemorrhage, purpura, and cutaneous bullae. one patient ate oysters 2 days before the onset of illness. vibrio vulnificus, a lactose-positive halophilic vibrio, was isolated from the ascitic and cutaneous fluid in both cases, and from the blood in one of the two cases. both isolated strains were sensitive to the antibiotics given to the patients from the beginning; however, both patients d ...	1985	3898820