Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| use of sodium dodecyl sulfate-polymyxin b-sucrose medium for isolation of vibrio vulnificus from shellfish. | the differential and selective sodium dodecyl sulfate-polymyxin b-sucrose medium (sps) of kitaura et al. (t. kitaura, s. doke, i. azuma, m. imaida, k. miyano, k. harada, and e. yabuuchii, fems microbiol. lett. 17:205-209, 1983), which highlights alkylsulfatase activity, was evaluated for its potential use in the direct isolation and enumeration of vibrio vulnificus from shellfish. v. vulnificus was detected by this method in six of nine shellfish samples collected from diverse geographic locales ... | 1987 | 3662506 |
| na+ modulates the k+ permeability and the membrane potential of alkalophilic bacillus. | in the absence of na+ in the medium, the membrane potential of obligately alkalophilic bacillus cells was found to be decreased by the addition of k+ to the medium, whereas k+ addition in the presence of na+ had no effect. rb+ showed essentially the same effect as k+. the decreased membrane potential was quickly restored by lowering the k+ concentration in the medium or by adding na+ or li+ to the medium. thus, in the absence of na+, the membrane potential of alkalophilic bacillus seems to be af ... | 1987 | 3663674 |
| vibrio vulnificus. | v vulnificus is a halophilic or salt-requiring vibrio that has been isolated repeatedly from seawater and shellfish in coastal waters. this vibrio, first described by hollis et al in 1976, can be differentiated from other similar vibrios by its ability to ferment lactose and by its lower tolerance for sodium chloride. v vulnificus, unlike most other vibrios, has seldom been incriminated as a cause of gastroenteritis but is a particularly virulent organism that causes severe wound infections in m ... | 1987 | 3667122 |
| new selective and differential medium for vibrio cholerae and vibrio vulnificus. | thiosulfate-citrate-bile salts-sucrose agar has been routinely used for the isolation of pathogenic vibrios, although its selectivity for vibrio cholerae and vibrio vulnificus is inadequate. therefore, a new plating medium, cellobiose-polymyxin b-colistin agar, was developed for the isolation of these two species. cellobiose-polymyxin b-colistin agar demonstrated a significant advantage over other media designed for the isolation or differentiation of vibrios: of both the 136 strains representin ... | 1987 | 3674873 |
| vibrio vulnificus bacteremia with endocarditis. | 1987 | 3686154 | |
| distribution of immunoreactive inhibin like material in different regions of human prostate. | distribution of immunoreactive inhibin material (ir-ilm) in different regions of human prostate was studied by using specific radio-immunoassay. mean ir-ilm concentrations in the central, peripheral and periurethral zones were 27.3 +/- 9.3, 2.4 +/- 0.7 and 3.0 +/- 1.5 micrograms/mg protein respectively (p central vs periurethral and peripheral less than 0.05). | 1987 | 3688474 |
| analysis of protein patterns from different organs and cell fractions of trisomy 19 mice. | proteins were extracted from liver, brain, and skin of 6-day-old mice with trisomy (ts) 19 and fractionated into solubilized cell proteins and structure-bound cell proteins. the proteins were separated by two-dimensional electrophoresis, and protein patterns were compared in the combinations ts/normal and normal/normal. analysis of the protein patterns revealed protein spots (variants) with densities higher (h-type) or lower (l-type) in trisomies than in normal mice. some of these variants were ... | 1987 | 3692480 |
| effect of iron and liver injury on the pathogenesis of vibrio vulnificus. | noncholera vibrio species can cause necrotizing soft tissue infections and cellulitis in addition to gastroenteritis. cellulitis and necrotizing soft tissue infections frequently occur in people with liver disease or in people who are in some way a compromised host. we studied the effect of vibrio vulnificus on the mortality of mice treated with carbon tetrachloride, an agent that causes liver damage, desferal, an iron-chelating agent, or iron. the ld50 of v. vulnificus is approximately 2.5 x 10 ... | 1987 | 3695454 |
| effect of pre- and postnatal exposure to alcohol on perivenous and periportal neonatal rat hepatocytes. | hepatocytes from 12-day-old rats, pre and postnatally exposed to alcohol and pair-fed controls, were isolated and subfractionated in six cell subpopulations on percoll density gradients. these cells were characterized using biochemical, stereological and cytofluorometric methods. our results show that the low density cells (f2) are mainly perivenous cells, whereas the heavier cells (f6) were primarily periportal cells. these results were confirmed by alanine aminotransferase (alat), and glutamat ... | 1987 | 3426691 |
| vibrio vulnificus infections in singapore. | 1987 | 3429797 | |
| liver cell lines. | 1987 | 3431441 | |
| [hypoxia and protection of the brain (isoflurane vs halothane and enflurane]. | 1987 | 3438012 | |
| destructive chronic borrelia meningoencephalitis in a child untreated for 15 years. | a case of borrelia spirochetosis with recurring episodes of progressively destructive chronic meningoencephalitis, untreated for 15 years, is presented. the patient, now 23 years old, represents an example of the natural course of the disease and may serve as a reminder of the diagnosis in retrospective cases as well as prospectively for acute and chronic states of serous meningitis/meningoencephalitis both in children and adults. | 1987 | 3441752 |
| monoclonal antibodies against the haemolysin of vibrio vulnificus. | the extracellular haemolysin produced by vibrio vulnificus strain fcc was partially purified from the culture supernate by sequential ammonium sulphate precipitation, gel filtration with sepharose 4b, and deae-sephacel ion-exchange column chromatography. using this semi-purified haemolysin as the antigen, several monoclonal antibodies (mabs) were established; they were all of the igg2b class with lambda light chains. one representative mab, 6f8d, completely neutralized the haemolytic activity an ... | 1987 | 3443854 |
| the role of catecholamines and glucagon on serum and liver metallothionein response to restraint stress. | the role of glucagon and catecholamines on serum and liver metallothionein (mt) concentrations in basal and stress conditions has been studied. glucagon showed no effect on serum mt either in basal (unstressed) or stress (20 hours of restraint) conditions. in contrast, glucagon administration increased both unstressed and stressed levels of liver mt. no effect of the alpha + beta-catecholamine blocker labetalol on serum mt levels was observed in unstressed rats. however, the administration of la ... | 1987 | 3448709 |
| variability of haemolysin(s) produced by vibrio vulnificus. | the peptide composition and antigenic cross-reactivity of partially purified and concentrated haemolysins of 16 strains of vibrio vulnificus were examined by sds-page and immunoblotting analysis, using a monoclonal antibody (mab), 6f8d, raised against the haemolysin. all strains produced a common peptide of 36 kda and the mab reacted with this peptide. in some strains, larger molecules, including a 56 kda peptide, were produced, but the mab did not react with this peptide. the haemolytic activit ... | 1987 | 3449599 |
| mechanism of haemolysis by vibrio vulnificus haemolysin. | the haemolytic action of vibrio vulnificus haemolysin (vvh) was compared to that of streptolysin o (slo). both were cholesterol-binding haemolysins, but differed in the release of haemoglobin (hb). in the first step of haemolysis, the haemolysins were temperature-independently bound to the cholesterol site on the target erythrocyte membrane. this was followed by the rapid release of k+, which is an intra-erythrocyte marker. hb was then released, in different ways. in the case of vvh, hb was rele ... | 1987 | 3449600 |
| intestinal helminthiases and human health: recent advances and future needs. | 1987 | 3294628 | |
| ca2+-binding parvalbumin in rat testis. characterization, localization, and expression during development. | parvalbumin, a ca2+-binding protein, was isolated from rat testis. this is the first demonstration of the protein in endocrine glands. by using a rat parvalbumin cdna probe, parvalbumin mrna was demonstrated in the testis, indicating that the protein is synthesized in this tissue and that testis parvalbumin is a product of the same gene as the one encoding for muscle parvalbumin. parvalbumin was localized by immunohistochemical methods in the leydig cells and in the acrosome region of maturing s ... | 1987 | 3294830 |
| purification and characterization of vibrio vulnificus protease. | a protease was purified from a strain of vibrio vulnificus isolated from the blood of a septicemic human. the vibrio was cultured in bacto peptone-yeast extract medium, and the protease was purified by a purification procedure including ultrafiltration of the culture supernatant with an amicon ym 5 membrane, diethylaminoethyl-sephacel column chromatography, sephacryl s-200 column chromatography and fast protein liquid chromatography on mono q column. the protease preparation revealed homogeneity ... | 1987 | 3295490 |
| effect of posttransplantation administration of peripheral blood lymphocytes in skin-grafted mice treated with antilymphocyte serum or antilymphocyte serum plus bone marrow. | posttransplantation administration of donor-specific bone marrow cells to als-treated allograft recipients produces graft survival that is greater than that produced by als treatment alone. we have now studied the effect of peripheral blood lymphocytes (pbls) in a mouse skin allograft model using this protocol (c3h skin grafted to b6af1 mice, day 0; i.p. als on days -1 and +2; i.v. bone marrow days +6 or +7). when pbls are injected in place of bone marrow cells, graft survival is extended beyond ... | 1987 | 3299924 |
| purification and characterization of an elastolytic protease of vibrio vulnificus. | large amounts of a highly purified, extracellular elastolytic protease of vibrio vulnificus were obtained by sequential ammonium sulphate precipitation and hydrophobic interaction chromatography with phenyl-sepharose cl-4b. the protease had an mr of about 50,500 (estimated by sds-page), a pi of 5.7, and a temperature optimum range of 55 to 60 degrees c. the ph optimum and the results of inactivation studies suggested that the enzyme was a neutral metalloprotease. the protease had about 429 amino ... | 1987 | 3312481 |
| vibrio vulnificus bacteremia: report of a case and review of the literature. | 1987 | 3316128 | |
| effect of acute renal failure on insulin disposition in the isolated perfused rat kidney. | the renal disposition of insulin in acute renal failure has not been evaluated. we used the isolated perfused rat kidney to test the hypothesis that acute renal failure (arf) decreases renal insulin clearance. we used warm ischemia for 45 min, uranyl nitrate 5 mg/kg, ureter ligation, and nonfiltering kidneys as methods of inducing arf. comparisons were made with normal control kidneys. the concentrations of insulin in perfusate and urine was determined by radioimmunoassay. acute renal failure ca ... | 1987 | 3318499 |
| omental pedicle graft in the management of infected ascending aortic prostheses. | two patients had mediastinal infections with chronic draining sinus tracts that involved a vascular prosthesis in the ascending aorta. in 1 patient, a false mycotic aneurysm developed and in the other, a beginning rupture of the proximal suture line. in both patients, the infection was cured by replacing the infected aortic prosthesis combined with wrapping the new prosthesis with a pedicled omental graft. an omental graft was used to protect the vascular prosthesis and minimize the risk of recu ... | 1987 | 3318738 |
| t cell factors involved in the isotype-specific regulation of the ige antibody response. | 1987 | 3333779 | |
| 1,4-dithiothreitol-induced changes in histamine h1-agonist efficacy and affinity in the longitudinal smooth muscle of guinea-pig ileum. | the effect of 1,4-dithiothreitol (dtt) on histamine h1-receptor agonist affinity and efficacy has been investigated in longitudinal muscle strips of guinea-pig ileum. exposure of ileal smooth muscle to dtt significantly increased the maximal responses to the partial agonists skf71473 and de-2pea, indicative of an increase in agonist efficacy. this effect was paralleled by a small decrease in ec50 values. in contrast, dtt produced a parallel displacement of the concentration-response curve to the ... | 1987 | 2880626 |
| glutathione metabolizing enzyme activities in human thyroid. | glutathione peroxidases, glutathione transferase, glutathione reductase and gamma-glutamyl transpeptidase activities were analyzed in human thyroid tissues obtained from 17 patients undergoing resectional surgery because of a malignancy. it was deduced, from measurements of glutathione peroxidase activity with both h202 and cumene hydroperoxide, that thyroid contains only the selenium enzyme. the absence of selenium independent glutathione peroxidase activity in thyroid was confirmed with gel fi ... | 1987 | 2883074 |
| absence of age-related changes in the binding of the beta adrenergic antagonist 125i-iodohydroxybenzylpindolol in rat heart. | the effect of age on the density and the affinity of beta adrenergic receptors was determined in the hearts of fischer 344 rats at three ages, 6, 12, and 24 months old. the binding of the beta adrenergic antagonist 125i-iodohydroxybenzylpindolol (ihyp), was used to quantitate and characterize cardiac beta adrenergic receptors. the maximal number of binding sites (bmax = f moles/mg of protein) were 26.3 +/- 2.5, 25.4 +/- 0.99, and 24.5 +/- 2.4 and the dissociation constants (kd = nm) were 0.166 + ... | 1987 | 2888126 |
| chlamydia trachomatis in antenatal clinics. | 1987 | 2889053 | |
| [h2-antihistaminics. 35. synthesis and h2-antagonistic action of imidazolylmethylthioalkyl-substituted 1,2,4-triazoles]. | 1987 | 2889442 | |
| a unique t-cell line derived from an htlv-1-negative adult t-cell leukemia patient. | a unique t-cell line, designated atl-5t, was established from lymphoma cells in pericardial effusion of an adult t-cell leukemia (atl) patient not carrying htlv-1 provirus. the cell line is okt4 and/or leu3a+ and okt8 and/or leu2a+, but interleukin 2 receptor (il2r)- and htlv-1 provirus genome negative, and has cytogenetically abnormal karyotypes. the cell line contains rearranged t-cell receptor beta-chain gene, which was identical in rearrangement pattern to the t-cell receptor beta-chain gene ... | 1987 | 2890613 |
| correlation between virulence and colony morphology in vibrio vulnificus. | of 38 isolates of vibrio vulnificus examined, all avirulent strains produced only translucent colonies. all virulent strains, with the exception of biogroup 2 (eel pathogens), exhibited both opaque and translucent colonies. isogenic morphotypes were examined for a variety of phenotypic and virulence traits. only the ability to utilize transferrin-bound iron and the presence of a surface polysaccharide were found to correlate with colony opacity and virulence. | 1987 | 2432016 |
| the presence of the 30 nm filament structure of chromatins in intact chicken erythrocytes observed by 31p nmr. | a 31p nmr spectrum of chicken erythrocyte chromatins in vivo was obtained. the spectrum was characterized by examining the spectra of isolated nuclei and nondigested chromatins under various conditions. the results have shown that most chromatins in chicken erythrocyte nuclei assume the 30 nm filament structure as a second step of condensation of dna in vivo. | 1987 | 2435577 |
| evolution and development of the outer acrosomal membrane (oam) and evidence that acrosin-inhibitors are proteins of the oam. | an antiserum to the purified porcine outer acrosomal membrane (oam) was raised in rabbits and the igg fraction isolated by ammonium sulphate precipitation and ion exchange chromatography. the antibodies reacted exclusively with the acrosomal cap of the sperm head as revealed by indirect immunofluorescence. in addition they cross-reacted not only with the acrosomal part of the spermatozoa of all mammalian species tested (bull, horse, rabbit, rat, mouse, hamster, mole, antelope, monkey, man) but a ... | 1987 | 2446533 |
| influence of neuropeptides on airway smooth muscle. | the first bronchoactive (and vasoactive) peptide to be discovered in the lung was isolated and characterized in 1970 from an embryologically related organ, the small intestine. since then, more than 20 additional peptides have been described in lung tissue and their biologic activities investigated. many of these are neuropeptides acting as neurotransmitters or neuromodulators to influence airway, pulmonary vascular, and other functions. more neuropeptides are known to exist in the brain and per ... | 1987 | 2446543 |
| [fatal septicemia of vibrio vulnificus in a patient with chronic hepatitis]. | 1987 | 2836645 | |
| the chicken carbonic anhydrase ii gene: evidence for a recent shift in intron position. | the complete nucleotide sequence of the coding region of the chicken carbonic anhydrase ii (ca ii) gene has been determined from clones isolated from a chicken genomic library. the sequence of a nearly full length chicken ca ii cdna clone has also been obtained. the gene is approximately 17 kilobase pairs (kb) in size and codes for a protein that is comprised of 259 amino acid residues. the 5' flanking region contains consensus sequences commonly associated with eucaryotic genes transcribed by r ... | 1987 | 3029691 |
| role of postnatal gonadal function in the determination of thyrotropin (tsh) releasing hormone-induced tsh response in adult male and female rats. | permanent effects of postnatal gonadal function on the hypothalamo-pituitary-thyroid axis were examined in male and female rats of the wistar-imamichi strain. animals were used at the age of about 10 weeks. neonatally castrated (nc) males showed a significantly higher plasma tsh response to trh (10 micrograms/kg bw, ip) than males castrated at 1, 2, 3, 4, or 6 weeks of age. the tsh response in intact males was similar to that seen in nc males. neonatal androgenization of nc males with 100 microg ... | 1987 | 3032567 |
| effects of inhibitors of the cytoplasmic structures and functions on the early phase of infection of cultured cells with simian virus 40. | to obtain information about cytoplasmic structures and functions involving the entry of simian virus 40 virions into cells, we examined whether the inhibitors that affect the functions and/or structure of lysosomes, cell membrane, and cytoskeletons inhibit expression of nuclear t antigen in the sv40-inoculated rat 3y1 and monkey cv-1 cells. chloroquine, methylamine, and butylamine did not inhibit t-antigen expression, suggesting that lysosomal acidification is not required for establishment of i ... | 1987 | 3033894 |
| maturation antigen of the mouse sperm flagellum: ii. origin from holocrine cells of the distal caput epididymis. | during epididymal transit, the mouse sperm flagellum acquires a surface glycoprotein (sma4) from epididymal fluid that functions as a sperm antiagglutinin. to determine the origin of this molecule, testes and epididymides of male mice were sectioned for light microscopy and stained with wheat germ agglutinin (wga)-peroxidase, a probe that has been used previously to examine the biology of sma4. wga reactivity was localized to the cytoplasm in a small population of cells in the distal caput epidi ... | 1987 | 3034104 |
| calmodulin-mediated cadmium inhibition of phosphodiesterase activity, in vitro. | ion-stripped bovine brain calmodulin (cam) binds 4 moles cd2+ as well as 4 moles ca2+ per mole protein, with similar affinity; in the presence of 1 mm mg2+ the molar binding ratio of cam for ca2+ decreased to 3, the apparent k0.5 for ca2+ nearly doubled, but the binding characteristics of cam for cd2+ were not changed. saturating concentrations ca2+ did not affect the molar binding ratio of cam for cd2+, but increased the apparent k0.5 for cd2+; vice versa, saturating concentrations cd2+ decreas ... | 1987 | 3034196 |
| radiation-induced dna damage and its repair. | application of modern methods of organic chemistry and recombinant dna technologies has provided new insights in the field of dna radiation damage and its repair. an overview of the chemical nature of the lesions inflicted on dna by ionizing radiation is presented. the structures of 29 different dna modified base or sugar residues are shown in comprehensive formation schemes. a fraction of radiation-induced modified bases is spontaneously released from the dna chain during irradiation. another p ... | 1987 | 3034813 |
| radiation-induced dna damage and its repair. | application of modern methods of organic chemistry and recombinant dna technologies has provided new insights in the field of dna radiation damage and its repair. an overview of the chemical nature of the lesions inflicted on dna by ionizing radiation is presented. the structures of 29 different dna modified base or sugar residues are shown in comprehensive formation schemes. a fraction of radiation-induced modified bases is spontaneously released from the dna chain during irradiation. another p ... | 1987 | 3034813 |
| identification of free radicals in myocardial ischemia/reperfusion by spin trapping with nitrone dmpo. | the spin trapping esr technique was applied to investigate oxygen-derived radicals in ischemic and post-ischemic rat hearts. using 5,5'-dimethyl-l-pyrroline-n-oxide, carbon-centered radicals were identified during ischemia and oxy-radical adducts (superoxide anion radical, o.-2 and hydroxyl radicals, .oh) in post-ischemic rat heart. the formation of these spin adducts was inhibited by superoxide dismutase, suggesting that superoxide plays a role in the adducts' formation. the results demonstrate ... | 1987 | 3040465 |
| examination of cardiac alpha-adrenoceptors from pharmacological responses and radioligand binding. comparison of rat and guinea pig tissues. | the object of this study was to determine suitable experimental conditions for the pharmacological evaluation of cardiac alpha-adrenoceptors. atrial and ventricular preparations of the guinea pig and rat were employed, and the alpha-adrenoceptor responsiveness was compared with the binding of the alpha-adrenoceptor radioligand [3h]prazosin in membranes prepared from these cardiac regions. the experimental variables examined were the pacing frequency, bath temperature, choice of agonist, and card ... | 1987 | 3041119 |
| [mechanisms of hypergastrinemia after vagotomy]. | 1987 | 2824916 | |
| [mechanisms of hypergastrinemia after vagotomy]. | 1987 | 2824916 | |
| dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites. | selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ... | 1987 | 2825926 |
| dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites. | selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ... | 1987 | 2825926 |
| overlapping distributions of receptors for atrial natriuretic peptide and angiotensin ii visualized by in vitro autoradiography: morphological basis of physiological antagonism. | atrial natriuretic peptides exert actions on many key organs involved in blood pressure and water and electrolyte balance. many of these actions result in a physiological antagonism of angiotensin. to investigate the morphological basis of this interaction, we have mapped the distribution of receptors for atrial natriuretic peptide and angiotensin ii in a number of target organs, using 125i-labelled rat atrial natriuretic peptide (99-126) and 125i-labelled [sar1,ile8]angiotensin ii. in the kidne ... | 1987 | 2825937 |
| cytomegalovirus pneumonia. | cytomegalovirus (cmv) is a common virus that asymptomatically infects the majority of the population by early adulthood. after initial infection, the virus remains in a latent state in the host and can be reactivated under a variety of conditions. in contrast to the normal host, both primary and reactivated symptomatic cmv infections are common in certain immunocompromised patients including organ transplant recipients and patients with the acquired immunodeficiency syndrome (aids). one of the m ... | 1987 | 2827281 |
| biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro. | osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ... | 1987 | 3502681 |
| biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro. | osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ... | 1987 | 3502681 |
| tissue-specific and developmental expression of human transthyretin gene in transgenic mice. | to analyze the regulation of transthyretin gene expression we have produced transgenic mice by microinjecting cloned human transthyretin genes into fertilized eggs of c57bl/6 mice. the 7.6-kilobase (kb) human transthyretin gene containing about 500 base pairs (bp) in the upstream region was used for microinjection. seven out of nine transgenic mice had detectable amounts of human transthyretin in serum when analyzed by enzyme-linked immunosorbent assay. transthyretin mrna was detected in liver a ... | 1987 | 3502973 |
| a quantitative morphological examination of bovine vulval skin glands. | in adult cows the volumes of sweat and sebaceous glands per unit skin surface area (ssa) in the vulva were greater (p less than 0.01) than in the neck, lip or eyelids. within this perineal region skin gland volumes were greater dorsally than ventrally (p less than 0.01). adult cows had much greater (p less than 0.01) volumes of perineal skin glands per unit ssa than 8 weeks or 6 weeks old heifer calves or steers. there was little or no difference between animal categories in neck skin gland dime ... | 1987 | 3503046 |
| phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells. | we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ... | 1987 | 3543022 |
| phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells. | we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ... | 1987 | 3543022 |
| the morphology and distribution of serotonin-like immunoreactive fibers in the cat dorsal column nuclei. | fibers showing serotonin-like immunoreactivity (5-ht-li) are demonstrated in the gracile, cuneate and external cuneate nuclei of the cat using avidin-biotinylated horseradish peroxidase and indirect fluorescence immunohistochemical methods. 5-ht-li fibers are located in all cytoarchitectural subdivisions of the gracile and cuneate nuclei but are restricted to the medial half of the external cuneate nucleus. in all nuclei, 5-ht-li fibers consist of long, varicose strands showing a wide range in t ... | 1987 | 3554006 |
| calcitonin gene-related peptide in the ventral tegmental area: selective modulation of prefrontal cortical dopamine metabolism. | the distribution of calcitonin gene-related peptide (cgrp) in the a10 dopamine (da) cell group region of the ventral tegmental area (vta) of the rat was examined using immunohistochemical techniques. cgrp-like immunoreactivity was localized to axons innervating the rostral and dorsal vta. direct administration of cgrp to the vta of the rat resulted in a dose-related increase in da utilization in the medial prefrontal cortex, but not other mesocortical, mesolimbic, or striatal da terminal field r ... | 1987 | 3554009 |
| differential effects of serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion. | the present study was designed to investigate the effect of distinct serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion. low doses of the selective 5-ht1a agonists 8-hydroxy-2-(di-n-propylamino)tetralin (8-oh-dpat) (5.0-500.0 micrograms/kg, i.p.) and ipsapirone (tvx q 7821; 0.5-2.5 mg/kg, i.p.), and of the 5-hydroxytryptamine (5-ht) agonist mk-212 (2.0 mg/kg, i.p.), did not elevate plasma renin activity (pra) and concentration (prc) 30 min postinjection. ... | 1987 | 2952898 |
| differential effects of serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion. | the present study was designed to investigate the effect of distinct serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion. low doses of the selective 5-ht1a agonists 8-hydroxy-2-(di-n-propylamino)tetralin (8-oh-dpat) (5.0-500.0 micrograms/kg, i.p.) and ipsapirone (tvx q 7821; 0.5-2.5 mg/kg, i.p.), and of the 5-hydroxytryptamine (5-ht) agonist mk-212 (2.0 mg/kg, i.p.), did not elevate plasma renin activity (pra) and concentration (prc) 30 min postinjection. ... | 1987 | 2952898 |
| experimental echinococcus granulosus infection in mice: immunocytochemical analysis of lymphocyte populations in local lymphoid infections during early infection. | the influence of subcutaneous infection with protoscoleces of echinococcus granulosus on the distribution of lymphocyte subsets in draining lymph nodes has been evaluated by immunocytochemical labelling of lymphocyte surface antigens. these studies reveal marked expansion of paracortical thy-1+, lyt-1+ cells and moderate proliferation of surface immunoglobulin-bearing b-cells immediately after infection. the lyt-1+:lyt-2+ ratio decreases rapidly during the first 21 days post-infection and remain ... | 1987 | 2956563 |
| ranking possible carcinogenic hazards. | this review discusses reasons why animal cancer tests cannot be used to predict absolute human risks. such tests, however, may be used to indicate that some chemicals might be of greater concern than others. possible hazards to humans from a variety of rodent carcinogens are ranked by an index that relates the potency of each carcinogen in rodents to the exposure in humans. this ranking suggests that carcinogenic hazards from current levels of pesticide residues or water pollution are likely to ... | 1987 | 3563506 |
| mouse skin damage caused by cytolysin from vibrio vulnificus and by v. vulnificus infection. | light and electron microscopy of mouse skin damage caused by intradermal infection with a virulent strain of vibrio vulnificus and by a single intradermal injection of the cytolytic toxin produced by the bacterium revealed similar structural alterations. the epidermis was intact; however, the infection and toxin produced acute cellulitis characterized by extensive extracellular edema; disorganization of collagen bundles; large accumulations of cell debris and plasma proteins; damaged or necrotic ... | 1987 | 3805762 |
| pneumonia--the quality of medical records data. | the quality of medical records data for patients who were hospitalized with community-acquired pneumonia was assessed by comparing medical records data with data obtained in a prospective study of pneumonia for the period april 1, 1984, to december 31, 1984. one hundred five patients fulfilled the case definition of pneumonia for entry into the prospective study. one hundred twenty-seven patients were identified by medical records data. seventy-three of the patients appeared in both studies. the ... | 1987 | 3807447 |
| comparison of the pharmacological properties of pituitary and biosynthetic human growth hormone. demonstration of antinatriuretic/antidiuretic and barbital sleep effects of human growth hormone in rats. | biosynthetic human growth hormone was compared with pituitary human growth hormone and pituitary 22 k in the weight gain and the tibia test. the three preparations were found to be equipotent. furthermore, the growth hormones were compared in various pharmacological test systems. all three preparations were found to have a marked antidiuretic and antinatriuretic effect in the rat and to cause a significant shortening of the hexobarbital sleeping time in mice. biosynthetic and pituitary preparati ... | 1987 | 3811769 |
| clinical and ecological characteristics of vibrio vulnificus in the northeastern united states. | multiple seawater sites in the northeastern united states, particularly long island sound, and shellfish from long island sound were sampled from april to november for 3 successive yr, 1983-1985. hospitals in coastal and metropolitan areas of connecticut were surveyed for the same 3-yr period, vibrio vulnificus can be found in these waters during the summer months. the appearance of these virulent bacteria in both seawater and shellfish are a function of the water temperature; no v. vulnificus c ... | 1987 | 3816129 |
| vibrio vulnificus infection after raw oyster ingestion in a patient with liver disease and acquired immune deficiency syndrome-related complex. | sepsis, peritonitis, and gastroenteritis developed in a 45-yr-old homosexual man 1 day after ingestion of raw oysters. the patient had chronic active hepatitis and cirrhosis with hepatitis b virus and delta-infection. he also had persistent generalized lymphadenopathy associated with htlv-iii antibody positivity. vibrio vulnificus was isolated from the patient's blood and peritoneal fluid as well as from the same batch of oysters at the restaurant where the patient had visited. to our knowledge, ... | 1987 | 3817400 |
| isolation and properties of a novel igg-binding protein from streptococci of serological group u. | a nonimmune binding of immunoglobulin (ig) g has been detected in streptococci of group u. the group u fc-binding site differed from the five previously known types of staphylococcal and streptococcal fc-binding sites by its strong affinity for murine igg, with dissociation constants in nanomolar range for rat and mouse igg, as well as for mouse igg subclasses 1, 2a, 2b and 3. it also differed from other binding sites by the high sensitivity towards trypsin. the fc-binding protein could be solub ... | 1987 | 3821654 |
| reaction to injectable collagen: results in animal models and clinical use. | since its commercial release, zyderm collagen implant has been used to treat more than 200,000 subjects in the united states for soft-tissue contour defects and more than 250,000 patients internationally (including the united states). approximately 3 percent of subjects' skin tested with zyderm collagen experience localized hypersensitivity reactions to collagen, whereas approximately 1 percent of treated patients demonstrate symptoms of hypersensitivity at treatment sites. of the latter treatme ... | 1987 | 3823250 |
| identification of environmental vibrio vulnificus isolates with a dna probe for the cytotoxin-hemolysin gene. | we screened 44 lactose-positive vibrio strains isolated from the marine environment for homology with a 3.2-kilobase dna fragment encoding the vibrio vulnificus cytotoxin-hemolysin gene. all 29 marine isolates identified as v. vulnificus on the basis of numerical taxonomy and dna-dna hybridization studies hybridized with the cytotoxin gene probe, as did all v. vulnificus reference strains. homologous gene sequences were identified in no other lactose-positive marine vibrio isolates nor in 10 oth ... | 1987 | 3827248 |
| nutrition in renal disease: the adult on dialysis. | 1987 | 260630 | |
| [environment and quality of sleep in the hospital milieu]. | 1987 | 260315 | |
| ultracentrifugal study of effect of sodium chloride on micelle size of fusidate sodium. | apparent micellar molecular weights were determined with the antibiotic fusidate sodium by ultracentrifugation in varying counterion concentrations (na+). the effects of buffer salts, ph, sodium chloride concentration, and drug concentration were studied. the results strongly support the concept of the formation of primary micelles composed of five monomer units, followed by aggregation of the pentomers into larger micelles as salt concentration increases. | 1986 | 239200 |
| a hydrogen-deuterium exchange study of the amide protons of polymyxin b by nuclear-magnetic-resonance spectroscopy. | 1. proton magnetic resonance spectra at 270 mhz of polymyxin b, a cationic oligopeptide antibiotic, show the influence of the inorganic counteranion present in solution. 2. hydrogen-deuterium exchange rates for the amide protons are of two types, depending on whether the anion is monovalent or polyvalent. polyvalent anions catalyse the acid-catalysed reaction more than the monovalent anions. 3. the structure in solution was monitored using the proton signals of the amides, the phenylalanine arom ... | 1986 | 203455 |
| [pharyngeal tonsils, mouth breathing and facial development]. | 1986 | 268664 | |
| [responsibility for the patient's welfare]. | 1986 | 260995 | |
| differential initiation of translation of a single estrogen receptor mrna could explain some estradiol resistance cases. | cell response to steroid stimulation is generally acknowledged to be mediated by an intracellular protein known as a receptor. response intensity is related to the affinity of the receptor and to the number of sites occupied by its specific ligand. although verified in the majority of experimental and clinical studies, certain phenomena of steroid hormone resistance would seem to challenge this assertion. application of gene molecular biology to determine the action mechanisms of steroid hormone ... | 1986 | 1306521 |
| different effects of the ionophore a-23187 and d-glucose on 45ca2+ fluxes in isolated islets of ob/ob-mice. | fluxes of 45ca2+ were studied in beta-cell rich islets of non-inbred ob/ob-mice, using lacl3 to wash out extra-cellular and superficially bound 45ca2+. the ionophore a-23187 (10 microm) increased the 45ca2+ uptake in islets both at 3 and 20 mm d-glucose, the effect being more pronounced after 10 min than after 120 min of incubation. in incubations for 120 min, 20 mm d-glucose induced a higher uptake of 45ca2+ than did a-23187. the ionophore enhanced the unidirectional efflux of 45ca2+ from prelo ... | 1986 | 362823 |
| processing-independent in vitro translocation of cytochrome p-450(scc) precursor across mitochondrial membranes. | in the presence of a membrane-permeable metal chelator, bovine adrenal cortex mitochondria imported p-450(scc) precursor without processing of the amino-terminal extension peptide. the imported precursor was bound to the matrix side surface of the inner membrane. when the inhibition due to the metal chelator was removed, the imported precursor was processed to the mature form. unprocessed precursor was also detected in mitochondria when the import reaction was carried out at relatively low tempe ... | 1986 | 3818579 |
| similarity principles and intrinsic geometries: contrasting approaches to interspecies scaling. | we criticize standard allometric approaches on the grounds that they emphasize scaling to one variable at a time, whereas chemically reactive hydrodynamic systems involved in pharmacokinetic phenomena are of higher dimension. we show that attempts based on mechanical similitude to set a dosage that would be equivalent across species (for example, from mouse to humans) lead to ambiguous results. another failing of standard allometry may be its incapability to accommodate the neoteny of homo sapie ... | 1986 | 3820095 |
| evaluation of the hereditary syrian hamster cardiomyopathy by 31p nuclear magnetic resonance spectroscopy: improvement after acute verapamil therapy. | the relation between metabolic and functional derangement in various cardiomyopathies has not been well characterized. this information was specifically sought in a spontaneous cardiomyopathic model. metabolic and hemodynamic parameters were obtained in glucose-perfused beating hearts of 180-200-day-old cardiomyopathic syrian hamsters and age-matched healthy animals. this period in the cardiomyopathic hamster lifetime is intermediary between the necrotic phase and the appearance of heart failure ... | 1986 | 3815756 |
| vibrio vulnificus septicemia: report of four cases. | 1986 | 3564544 | |
| [a case of sepsis, shock, disseminated intravascular coagulation, and necrotizing fasciitis caused by vibrio vulnificus treated with early debridement]. | 1986 | 3559356 | |
| rhamnetin-3-o-neohesperidoside, a new flavonoid from the leaves of derris trifoliata. | 1986 | 3509960 | |
| potentiation of hemorrhage-evoked catecholamine release by prior blood loss in cats. | the effect of prior blood loss on the plasma catecholamine response to acute hemorrhage (h) was assessed in alpha-chloralose-urethane anesthetized cats. animals sustained an initial h period of 0 (samples only), 10, or 20% h total blood volume. ninety minutes after reinfusion of the shed blood, all animals sustained a rapid 20% h. the catecholamine and arterial pressure responses to this second 20% h were assessed every 2 min for 20-min duration. plasma norepinephrine increased modestly in the 0 ... | 1986 | 3510559 |
| escherichia coli as cause of ecthyma gangrenosum. | 1986 | 3511469 | |
| feasibility of same-day identification of members of the family vibrionaceae by the api 20e system. | sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ... | 1986 | 3517061 |
| immunocytochemical localization of cathepsin b in rat kidney. ii. electron microscopic study using the protein a-gold technique. | thin sections of lowicryl k4m-embedded materials were labeled with protein a-gold complex. gold particles representing the antigen sites for cathepsin b were exclusively confined to lysosomes of each segment of the nephron. the heaviest labeling was noted in the lysosomes of the s1 segment of the proximal tubules. labeling intensity varied considerably with the individual lysosomes. lysosomes of the other tubular segments, such as the s2 and s3 segments of the proximal tubules, distal convoluted ... | 1986 | 3519753 |
| production of extracellular enzymes and cytotoxicity by vibrio vulnificus. | thirty-three strains of vibrio vulnificus of clinical and environmental origin were examined for production of 12 extracellular enzymes of potential importance to the virulence of this bacterium. strains of vibrio vulnificus were consistent in their production of protease, mucinase, lipase, chondroitinase, hyaluronidase, dnase, sulfatase, and hemolysin. no differences between clinical and environmental isolates were noted. although none of the enzymes appeared to correlate with the ability of th ... | 1986 | 3522090 |
| chitinase determinants of vibrio vulnificus: gene cloning and applications of a chitinase probe. | to initiate study of the genetic control of chitinolytic activity in vibrios, the chitobiase gene was isolated by cloning chromosomal dna prepared from vibrio vulnificus. chimeric plasmids were constructed from sau3a i partial digests of chromosomal dna by ligating 5 to 15-kilobase fragments into the bamhi site, i.e., in the tcr gene, of pbr322 (amr tcr). the resulting plasmids were transformed into escherichia coli dh1. chitobiase activity of the insert-bearing clones was detected by using a ch ... | 1986 | 3524459 |
| hemorrhagic bullae associated with vibrio vulnificus septicemia. report of two cases. | bullous lesions associated with vibrio vulnificus infection developed in two patients, both of whom had hepatic cirrhosis. one patient had a recent history of ingestion of raw oysters, while the other patient had recently exposed skin lacerations to sea water. both patients died within 24 hours of hospitalization, in spite of antibiotic treatment. vibrio vulnificus was isolated from blood and bullae in both patients. histologic examination of skin biopsy specimens revealed epidermal/dermal separ ... | 1986 | 3524472 |
| degenerative lesions of a coronary chemoreceptor and nearby neural elements in the hearts of victims of sudden death. | a coronary chemoreceptor and its neighboring nerves and ganglia were studied by serial sections of tissue taken from the hearts of sixteen victims of sudden unexpected death. either focal or extensive inflammatory destruction was present in the chemoreceptors of 13 of the 16 hearts, and similar abnormalities of local nerves and ganglia were present in all 16 hearts. since an identical coronary chemoreceptor in the dog has previously been shown to be the site of origin of a powerful reflex with m ... | 1986 | 3837961 |
| esculin hydrolysis by vibrio vulnificus. | a clinical isolate of vibrio vulnificus was found to hydrolyze esculin when tested on bile-esculin-azide agar during the initial characterization of the strain. reports in the literature of esculin hydrolysis by v. vulnificus are conflicting. we tested herein 52 strains of v. vulnificus from clinical and environmental sources for the ability to hydrolyze esculin. seventy-eight percent of the strains hydrolyzed esculin on bile-esculin-azide agar, whereas all strains of v. vulnificus tested were p ... | 1986 | 3943291 |
| comparison of particle-associated progesterone and oxytocin in the ovine corpus luteum. | the subcellular distribution of progesterone and oxytocin within the ovine corpus luteum was investigated using differential and density gradient centrifugation. progesterone and oxytocin were associated with particles which sedimented to a density of 1.049-1.054 g/ml and 1.054-1.061 g/ml respectively. particle-associated progesterone did not, however, display physical or biochemical characteristics consistent with its storage within secretory granules. when particle-associated progesterone was ... | 1986 | 3944530 |
| gastroenteritis in patients with stool isolates of vibrio vulnificus. | vibrio vulnificus, a marine vibrio associated with severe extraintestinal infections, has not been previously implicated as a cause of infectious diarrhea. three patients were identified with diarrheal illness from whom this organism was the sole pathogen recovered from cultured stool specimens. all three had eaten raw oysters within one week of becoming ill. these patients were all taking medication that reduces gastric acidity, two were heavy drinkers of alcohol, and one had unrecognized colon ... | 1986 | 3946453 |
| detection of anti-vibrio vulnificus cytolysin antibodies in sera from mice and a human surviving v. vulnificus disease. | an enzyme-linked immunosorbent assay and a cytolysin neutralization assay were used detect anti-vibrio vulnificus cytolysin antibodies in sera from mice and a human that survived v. vulnificus disease. the detection of antibodies against the cytolysin indicated that the cytolysin is produced in vivo, and this observation is consistent with the hypothesis that the cytolysin is involved in the pathogenesis of v. vulnificus disease. | 1986 | 3949387 |
| inland presentation of vibrio vulnificus primary septicemia and necrotizing fasciitis. | 1986 | 3953073 | |
| anal submucosal injection: a new route for drug administration in pelvic malignancies. part i. experimental study of misonidazole distribution in serum and tissues, with special reference to urinary bladder. preliminary report. | the anal and oral administration routes were compared in 30 rats to study the distribution of misonidazole, a radiation sensitizer, in the serum and tissues with special reference to the urinary bladder. 14c-labelled misonidazole was administered in a dose of 0.2 ml water/100 gm body weight containing 1 mu ci misonidazole. the dose was given orally by stomach tube in 15 rats, and was injected in the submucosa of the anal canal in another 15. animals were then killed after 15, 30, 60, or 120 minu ... | 1986 | 3957602 |