Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| sepsis in the newborn. | infections are the single largest cause of neonatal deaths globally. according to national neonatal perinatal database (2002-03), the incidence of neonatal sepsis in india was 30 per 1000 live-births; klebsiella pneumoniae and staphylococcus aureus were the two most common organisms isolated. based on the onset, neonatal sepsis is classified into two major categories: early onset sepsis, which usually presents with respiratory distress and pneumonia within 72 hours of age and late onset sepsis, ... | 2008 | 18376095 |
| osteomyelitis in military personnel wounded in iraq and afghanistan. | orthopedic injuries occurring in operations iraqi freedom and enduring freedom (oif/oef) are complicated by infections with multidrug resistant bacteria. we describe demographics and microbiology of oif/oef casualties with primary and recurrent osteomyelitis. | 2008 | 18376160 |
| incidence and characteristics of hospital-acquired pneumonia in a pulmonary rehabilitation setting. | the incidence of hospital acquired pneumonia (hap) varies according to the setting. it is estimated to be approximately 0.5% to 1% in hospitalized subjects but higher in mechanically ventilated patients in intensive care units. the incidence of hap in a pulmonary rehabilitation unit has not been investigated. | 2008 | 18376347 |
| epidemiology of gram-negative conjunctivitis in neonatal intensive care unit patients. | to describe the epidemiologic features, risk factors, and antibiotic susceptibilities for gram-negative conjunctivitis among neonatal intensive care unit (nicu) patients. | 2008 | 18378213 |
| assessing genetic heterogeneity within bacterial species isolated from gastrointestinal and environmental samples: how many isolates does it take? | strain typing of bacterial isolates is increasingly used to identify sources of infection or product contamination and to elucidate routes of transmission of pathogens or spoilage organisms. usually, the number of bacterial isolates belonging to the same species that is analyzed per sample is determined by convention, convenience, laboratory capacity, or financial resources. statistical considerations and knowledge of the heterogeneity of bacterial populations in various sources can be used to d ... | 2008 | 18378649 |
| properties and expression of a multidrug efflux pump acrab-kocc from klebsiella pneumoniae. | we previously reported that we had cloned genes responsible for multidrug resistance from the chromosomal dna of klebsiella pneumoniae mgh78578 using a drug-hypersusceptible escherichia coli strain as a host. one of the recombinant plasmids petv6 conferred resistance to host cells against a wide range of antimicrobial agents, dyes and detergents. it was revealed that this plasmid carried the acrbkp gene and a part of the acrakp gene coding for a multidrug efflux pump belonging to the rnd family. ... | 2008 | 18379044 |
| clinical outcome and prognostic factors of patients with pyogenic liver abscess requiring intensive care. | despite improvements in diagnosis and treatment, pyogenic liver abscess remains a life-threatening disease. this study evaluated clinical outcome and prognostic factors in patients with pyogenic liver abscess admitted to the intensive care unit. | 2008 | 18379245 |
| the incidence of infectious diseases after renal transplantation: a single-centre experience. | this single-centre study was designed to investigate the incidence of infections and their causative pathogens during the first three months after renal transplantation (rtx) in patients who had undergone the procedure in 2005 (n=174). we compared this group of patients with a previous one (1998-2000, n=437). in 2005, infection was diagnosed in 82 patients (47%). symptomatic lower urinary tract infection (uti) was present in 43 patients (25%), pyelonephritis in 15 (8.6%), and urosepsis in 7 (4%) ... | 2008 | 18160263 |
| effects of ethanol on cytokine production after surgery in a murine model of gram-negative pneumonia. | both alcohol abuse and surgery have been shown to impair immune function. the frequency of postoperative infectious complications is 2- to 5-fold increased in long-term alcoholic patients, leading to prolonged hospital stay. following surgery, an increase in interleukin (il)-6 has been shown to be associated with increased tissue injury and interleukin 1-(il-10) is known to represent an anti-inflammatory signal. the purpose of this study was to test the hypothesis that several days of excess alc ... | 2008 | 18162079 |
| epidemiology and clinical outcomes of community-acquired pneumonia in adult patients in asian countries: a prospective study by the asian network for surveillance of resistant pathogens. | appropriate antimicrobial treatment of community-acquired pneumonia (cap) should be based on the distribution of aetiological pathogens, antimicrobial resistance of major pathogens, clinical characteristics and outcomes. we performed a prospective observational study of 955 cases of adult cap in 14 hospitals in eight asian countries. microbiological evaluation to determine etiological pathogens as well as clinical evaluation was performed. bronchopulmonary disease (29.9%) was the most frequent u ... | 2008 | 18162378 |
| community-acquired klebsiella oxytoca causing splenic abscess. | 2008 | 18164642 | |
| oral carriage of yeasts and coliforms in stroke sufferers: a prospective longitudinal study. | to investigate prospectively the qualitative and quantitative changes in oral carriage of yeasts and coliforms in southern chinese people suffering from stroke. | 2008 | 18173450 |
| type 3 fimbriae, encoded by the conjugative plasmid pola52, enhance biofilm formation and transfer frequencies in enterobacteriaceae strains. | the conjugative plasmid pola52, which confers resistance to olaquindox and other antimicrobial agents through a multidrug efflux pump, was investigated for its ability to promote biofilm formation in escherichia coli. screening of a transposon-mutagenized pola52 clone library revealed several biofilm-deficient mutants, which all mapped within a putative operon with high homology to the mrkabcdf operon of klebsiella pneumoniae, where these genes are responsible for type 3 fimbriae expression, att ... | 2008 | 18174137 |
| effectiveness of glycerol mono-oleate as a biosealant. | the number of femoral artery catheterizations will increase over the next decade to more than 9 million worldwide. accordingly, a new era of access site management with vascular closure techniques utilizing biologics are being developed and implemented. glycerol mono-oleate (gmo) is one such biologic - a biodegradable compound that changes from a solid phase to a bioadhesive swollen semisolid phase when exposed to aqueous solutions and heat. we assessed whether gmo would: 1) achieve hemostasis m ... | 2008 | 18174616 |
| identification of the 4-hydroxycinnamate decarboxylase (pad) gene of klebsiella oxytoca. | a 7.1-kbp dna fragment isolated from a wild strain of klebsiella oxytoca was sequenced, leading to the identification of 10 open-reading frames (orfs), including a 504-bp pad gene. the pad gene of the gram-negative bacterium was subsequently expressed in escherichia coli as a chimeric pad. the deduced amino acid (aa) sequence of the pad gene from wild-type k. oxytoca showed approximately 50% homology to those of other bacterial pads from gram-positive bacilli plus a coccus. these data and a geno ... | 2008 | 18175898 |
| resistance in gram-negative bacilli in a cardiac intensive care unit in india: risk factors and outcome. | the objective of this study was to compare the risk factors and outcome of patients with preexisting resistant gram-negative bacilli (gnb) with those who develop sensitive gnb in the cardiac intensive care unit (icu). of the 3161 patients ( n=3,161) admitted to the icu during the study period, 130 (4.11%) developed health care-associated infections (hais) with gnb and were included in the cohort study. pseudomonas aeruginosa (37.8%) was the most common organism isolated followed by klebsiella sp ... | 2008 | 18182755 |
| necrotizing fasciitis: a life-threatening clinical disorder in uncontrolled type 2 diabetic patients. | we presented 23 patients with necrotizing fasciitis (nf), 15 of whom had uncontrolled diabetes mellitus (dm), for risk factors, clinical signs, laboratory findings and prognosis during the period 1998 and 2006 in istanbul. a hospital incidence of nf was 14.2/100,000 admissions. other risk factors were obesity in 9 and recent surgical trauma in 10 patients. the mean age of the patients with dm was higher than that of the patients with non-dm (58.6+/-12.8 vs 43.0+/-17.2 years, p=0.028). the most f ... | 2008 | 18190995 |
| [resistance to third generation cephalosporins in sfax hospitals, tunisia (1999-2005)]. | the authors wanted to assess the prevalence and to monitor the trends of resistance to broad-spectrum cephalosporins among various species of enterobacteria in the region of sfax (tunisia). | 2008 | 18191516 |
| the effect of control group selection in the analysis of risk factors for extended spectrum beta-lactamase-producing klebsiella pneumoniae infections. a prospective controlled study. | the aim was to evaluate the effect of control selection on risk factor analysis for extended-spectrum beta-lactamase-producing klebsiella pneumoniae (esbl-kp) infections. four contemporaneous case-control studies were conducted prospectively with 372 patients: study 1 (esbl-kp-infected vs non-infected); study 2 (esbl-kp-infected vs non-esbl-kp-infected); study 3 (all kp-infected vs non-infected); study 4 (non-esbl-kp-infected vs non-infected). time at risk (tar, i.e. duration of hospital stay) w ... | 2008 | 18192077 |
| enterobacter cloacae and klebsiella pneumoniae isolates producing ctx-m-15 recovered from hospital environmental surfaces from algeria. | 2008 | 18192081 | |
| changing epidemiology of adult bacterial meningitis in southern taiwan: a hospital-based study. | many factors may influence the epidemiologic trend of adult bacterial meningitis (abm). the objective of this study was to analyze recent epidemiologic trends of abm in order to provide a better therapeutic strategy. | 2008 | 18193387 |
| transgenic tissue-type plasminogen activator expression improves host defense during klebsiella pneumonia. | severe pneumonia is associated with a local inhibition of fibrinolysis in the lung as reflected by strongly reduced pulmonary plasminogen activator activity.objectives: to study the effect of elevation of local plasminogen activator activity during pneumonia caused by the common respiratory pathogen klebsiella pneumoniae. | 2008 | 18194423 |
| clinical and microbiological outcomes of serious infections with multidrug-resistant gram-negative organisms treated with tigecycline. | eighteen patients received tigecycline as treatment for infection due to multidrug-resistant gram-negative bacilli, including acinetobacter baumannii and klebsiella pneumoniae carbapenemase- and extended-spectrum beta-lactamase-producing enterobacteriaceae. pretherapy minimum inhibitory concentration values for tigecycline predicted clinical success. observed evolution of resistance during therapy raises concern about routine use of tigecycline in treatment of such infections when other therapie ... | 2008 | 18199038 |
| outbreak caused by a multidrug-resistant klebsiella pneumoniae clone carrying blavim-12 in a university hospital. | from november 2006 to april 2007, nine nonrepetitive isolates of klebsiella pneumoniae with reduced susceptibility or resistance to carbapenems were recovered from clinical specimens from separate patients hospitalized in a tertiary care hospital. the imipenem-edta synergy test was positive for all isolates. pcr, sequencing, and transferability experiments revealed the novel bla(vim-12) metallo-beta-lactamase gene, which was plasmid mediated and located in a class 1 integron. pulsed-field gel el ... | 2008 | 18199780 |
| antimicrobial susceptibility of multidrug-resistant gram negative bacteria to fosfomycin. | we evaluated the antimicrobial activity of fosfomycin against a randomly selected sample of 30 klebsiella pneumoniae, 30 pseudomonas aeruginosa, and 30 acinetobacter baumannii multidrug-resistant, clinical isolates from patients in a general tertiary care hospital in athens, greece. standard laboratory methods were used for susceptibility testing to commonly used antibiotics and the detection of extended-spectrum-beta-lactamase (esbl) and metallo-beta-lactamase (mbl) production. the minimum inhi ... | 2008 | 18214558 |
| phenotypic and molecular characterization of 5 novel ctx-m enzymes carried by klebsiella pneumoniae and escherichia coli. | the aim of the present study was to study the phenotypic and molecular characterization of 5 novel ctx-m-beta-1actamases carried by 5 klebsiella pneumoniae isolates and 3 escherichia coli isolates collected from 4 hospitals in hefei, china. | 2008 | 18215351 |
| in vitro activity profile of ceftobiprole, an anti-mrsa cephalosporin, against recent gram-positive and gram-negative isolates of european origin. | to determine the in vitro activity profile of ceftobiprole, a pyrrolidinone cephalosporin with activity against methicillin-resistant staphylococcus aureus (mrsa), for use as a contemporary baseline to help detect any changes in its activity profile throughout the course of clinical development and use. | 2008 | 18218646 |
| molecular subtyping of mastitis-associated klebsiella pneumoniae isolates shows high levels of diversity within and between dairy herds. | despite advances in controlling mastitis (inflammation of the mammary gland), udder infections caused by klebsiella pneumoniae continue to affect dairy cattle. mastitis caused by k. pneumoniae responds poorly to antibiotic treatment, and as a consequence, infections tend to be severe and long lasting. we sought to determine whether a nonrandom distribution of specific genotypes of k. pneumoniae was associated with mastitis from 6 dairy herds located in 4 different states. a total of 635 isolates ... | 2008 | 18218741 |
| qnra prevalence in extended-spectrum beta-lactamase-positive enterobacteriaceae isolates from turkey. | quinolone resistance mostly originates from chromosomal mutations. in recent years, however, plasmid-mediated quinolone resistance has been reported in several parts of the world. plasmid-borne qnra, qnrb, or qnrs genes are responsible for this kind of resistance. little is known about the diversity, type, and species range of the qnr genes in turkey. we screened qnra, qnrb, and qnrs genes in quinolone-resistant blood culture isolates collected from six different medical centers in turkey which ... | 2008 | 18219128 |
| evaluation of different laboratory tests for the detection of metallo-beta-lactamase production in enterobacteriaceae. | clinical isolates of klebsiella pneumoniae (91), escherichia coli (49), enterobacter spp. (27), proteus mirabilis (17), citrobacter freundii (2), providencia stuartii (3) and serratia spp. (5), with various mics of imipenem, were examined for production of metallo-beta-lactamases (mbls) with different phenotypic laboratory tests that have been previously published to detect mbls in pseudomonas aeruginosa and acinetobacter spp. | 2008 | 18222954 |
| 16s ribosomal rna methylase rmtd produced by klebsiella pneumoniae in brazil. | 2008 | 18222955 | |
| the current state of multidrug-resistant gram-negative bacilli in north america. | although much of today's media focuses on multidrug-resistant gram-positive bacteria such as methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus, resistance within gram-negative bacilli continues to rise, occasionally creating situations in which few or no antibiotics that retain activity are available. extended-spectrum beta-lactamase (esbl)-producing escherichia coli and klebsiella sp are emerging threats nationally. although carbapenems are considered the antibio ... | 2008 | 18225969 |
| synergistic antibacterial activity between thymus vulgaris and pimpinella anisum essential oils and methanol extracts. | essential oils (eos) and methanol extracts obtained from aerial parts of thymus vulgaris and pimpinella anisum seeds were evaluated for their single and combined antibacterial activities against nine gram-positive and gram-negative pathogenic bacteria: staphylococcus aureus, bacillus cereus, escherichia coli, proteus vulgaris, proteus mirabilis, salmonella typhi, salmonella typhimurium, klebsiella pneumoniae and pseudomonas aeruginosa. the essential oils and methanol extracts revealed promising ... | 2008 | 18226481 |
| genetic structures at the origin of acquisition of the beta-lactamase bla kpc gene. | genetic structures surrounding the carbapenem-hydrolyzing ambler class a bla kpc gene were characterized in several kpc-positive klebsiella pneumoniae and pseudomonas aeruginosa strains isolated from the united states, colombia, and greece. the bla kpc genes were associated in all cases with transposon-related structures. in the k. pneumoniae yc isolate from the united states, the beta-lactamase bla kpc-2 gene was located on a novel tn3-based transposon, tn4401. tn4401 was 10 kb in size, was del ... | 2008 | 18227185 |
| a five-year study in iran of ophthalmia neonatorum: prevalence and etiology. | the aim was to determine the prevalence of conjunctivitis and its bacteriological causes in neonates hospitalized in two hospitals in iran and to evaluate the maternal and newborns' data in conjunctivitis cases. | 2008 | 18227767 |
| linkage of acquired quinolone resistance (qnrs1) and metallo-beta-lactamase (blavim-1) genes in multiple species of enterobacteriaceae from bolzano, italy. | twenty-four of 209 oxyimino-cephalosporin- and/or aztreonam-resistant enterobacteriaceae collected around bolzano had reduced susceptibility or resistance to carbapenems and gave positive metallo-beta-lactamase (mbl) tests. their resistance mechanisms were investigated. | 2008 | 18184647 |
| [omphalitis with fatal outcome in new-born baby boy]. | omphalitis is a serious condition with important morbidity and mortality, especially in developing countries. the most commonly involved micro-organisms are staphylococcus aureus, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa. we describe a danish patient with cellulitis, severe septicaemia, and portal vein thrombosis as fatal complications of omphalitis. in spite of early recognition and prompt treatment in this case, it was not possible to save the child. | 2008 | 18208734 |
| efficacy of ertapenem for consolidation therapy of extended-spectrum beta-lactamase-producing gram-negative infections: a case series report. | infections caused by extended-spectrum beta-lactamase (esbl)-producing gram-negative organisms are becoming increasingly common and present significant challenges in terms of treatment. carbapenems is the antibiotic class of choice for treatment of these types of infections. ertapenem is the newest carbapenem, capable of being dosed once daily, and has some in vitro but little in vivo evidence supporting its use for the treatment of these resistant infections. | 2008 | 18230703 |
| microbiology and management of neonatal necrotizing enterocolitis. | necrotizing enterocolitis (nec) is a clinical syndrome of ischemic necrosis of the bowel of multiple etiological factors that include the presence of intestinal ischemia, abnormal bacterial flora, and intestinal mucosal immaturity. numerous reports have implied that the fecal microflora may contribute to the pathogenesis of nec. a broad range of organisms generally found in the distal gastrointestinal tract have been recovered from the peritoneal cavity and blood of infants with nec. the predomi ... | 2008 | 18236362 |
| characterization of metal binding in the active sites of acireductone dioxygenase isoforms from klebsiella atcc 8724. | the two acireductone dioxygenase (ard) isozymes from the methionine salvage pathway of klebsiella atcc 8724 present an unusual case in which two enzymes with different structures and distinct activities toward their common substrates (1,2-dihydroxy-3-oxo-5-(methylthio)pent-1-ene and dioxygen) are derived from the same polypeptide chain. structural and functional differences between the two isozymes are determined by the type of m2+ metal ion bound in the active site. the ni2+-bound niard catalyz ... | 2008 | 18237192 |
| the characterization of functions involved in the establishment and maturation of klebsiella pneumoniae in vitro biofilm reveals dual roles for surface exopolysaccharides. | the ability to form biofilm is seen as an increasingly important colonization strategy among both pathogenic and environmental klebsiella pneumoniae strains. the aim of the present study was to identify abiotic surface colonization factors of k. pneumoniae using different models at different phases of biofilm development. a 2200 k. pneumoniae mutant library previously obtained by signature-tagged mutagenesis was screened in static and dynamic culture models to detect clones impaired at early and ... | 2008 | 18237304 |
| fluoroquinolone resistance in pediatric bloodstream infections because of escherichia coli and klebsiella species. | in pediatric bloodstream infections with fluoroquinolone (fq)-resistant escherichia coli and klebsielia species, we noted an association between fq resistance and extended-spectrum beta-lactamase (esbl) production (or, 12; 95% ci: 2.28-83.8). a case control study revealed no significant risk factors (including prior antibiotic use) for fq resistance among esbl e coli and klebsiella species (esbl-ek). | 2008 | 18241740 |
| structure of the minor pseudopilin epsh from the type 2 secretion system of vibrio cholerae. | many gram-negative bacteria use the multi-protein type ii secretion system (t2ss) to selectively translocate virulence factors from the periplasmic space into the extracellular environment. in vibrio cholerae the t2ss is called the extracellular protein secretion (eps) system,which translocates cholera toxin and several enzymes in their folded state across the outer membrane. five proteins of the t2ss, the pseudopilins, are thought to assemble into a pseudopilus, which may control the outer memb ... | 2008 | 18241884 |
| surgical treatment of chronic otitis media with temporomandibular joint involvement. | we report a case of chronic otitis media with spread of the infection into the temporomandibular joint (tmj), causing displacement of the joint and malocclusion. chemotherapy of the malignant disease caused activation of the chronic ear infection with a temporomandibular joint involvement. the patient was operated twice to eradicate the infection from the mastoid, middle ear and temporomandibular joint. a radical cavity with musculoperiosteal flap obliteration was created. the joint was isolated ... | 2008 | 18242031 |
| diabetic foot osteomyelitis. | bone infection in the diabetic foot is always a complication of a preexisting infected foot wound. prevalence can be as high as 66%. diagnosis can be suspected in two mains conditions: no healing (or no depth decrease) in spite of appropriate care and off-loading, and/or a visible or palpated bone with a metal probe. the first recommended diagnostic step is to perform (and if necessary to repeat) plain radiographs. after a four-week treatment period, if plain radiographs are still normal, suspic ... | 2008 | 18242114 |
| risk factors for endogenous klebsiella endophthalmitis in patients with klebsiella bacteraemia: a case-control study. | to identify risk factors for klebsiella endophthalmitis in patients with klebsiella sepsis. | 2008 | 18245273 |
| nosocomial bloodstream infection in a pediatric intensive care unit. | to study the incidence of nosocomial blood stream infections (bsi) in a pediatric intensive care unit (picu) of a tertiary care teaching hospital, identify the organisms responsible and the pattern of antibiotic resistance over one decade. | 2008 | 18245931 |
| fluorescent in situ hybridization and flow cytometry as tools to evaluate the treatments for the control of slime-forming enterobacteria in paper mills. | slime formation is a serious problem nowadays in the paper industry. some enterobacteria are associated with the formation of slime deposits in paper and board mills. detection and characterization of slime forming bacteria, belonging to the genus enterobacter, raoultella, and klebsiella have been achieved by fluorescence in situ hybridization (fish), using one probe based on the enterobacterial repetitive intergenic consensus sequence and other two rrna targeted oligonucleotide probes. the effe ... | 2008 | 18247026 |
| pneumocephalus as a complication of multidrug-resistant klebsiella pneumoniae meningitis. | pneumocephalus implies air inside the cranial vault, which usually results from cranio-facial trauma. occasionally, meningitis caused by gas-forming organisms can result in pneumocephalus. klebsiella pneumoniae meningitis can, on rare occasions, cause pneumocephalus as a complication. the drug of choice for k. pneumoniae meningitis is a third-generation cephalosporin, and resistance to these drugs is unusual. we report a case of multidrug-resistant k. pneumoniae meningitis resulting from chronic ... | 2008 | 18249312 |
| molecular and descriptive epidemiologyof multidrug-resistant enterobacteriaceae in hospitalized infants. | to investigate the epidemiology of multidrug-resistant enterobacteriaceae (mdre) in hospitalized infants. | 2008 | 18251685 |
| a ligand-induced switch in the periplasmic domain of sensor histidine kinase cita. | sensor histidine kinases of two-component signal-transduction systems are essential for bacteria to adapt to variable environmental conditions. however, despite their prevalence, it is not well understood how extracellular signals such as ligand binding regulate the activity of these sensor kinases. cita is the sensor histidine kinase in klebsiella pneumoniae that regulates the transport and anaerobic metabolism of citrate in response to its extracellular concentration. we report here the x-ray ... | 2008 | 18258261 |
| endotoxemia does not limit energy supply in exercising rat skeletal muscle. | although depletion in high-energy phosphorylated compounds and mitochondrial impairment have been reported in septic skeletal muscle at rest, their impact on energy metabolism has not been documented during exercise. in this study we aimed to investigate strictly gastrocnemius muscle function non-invasively, using magnetic resonance techniques in endotoxemic rats. endotoxemia was induced by injecting animals intraperitoneally at t(0) and t(0) + 24 h with klebsiella pneumoniae lipopolysaccharides ... | 2008 | 18260074 |
| histidine-alpha143 assists 1,2-hydroxyl group migration and protects radical intermediates in coenzyme b12-dependent diol dehydratase. | diol dehydratase of klebsiella oxytoca contains an essential histidine residue. its x-ray structure revealed that the migrating hydroxyl group on c2 of substrate is hydrogen-bonded to hisalpha143. mutant enzymes in which hisalpha143 was mutated to another amino acid residue were expressed in escherichia coli, purified, and examined for enzymatic activity. the halpha143q mutant was 34% as active as the wild-type enzyme. halpha143a and halpha143l showed only a trace of activity. kinetic analyses i ... | 2008 | 18260646 |
| seasonal variation in klebsiella pneumoniae bloodstream infection on 4 continents. | klebsiella pneumoniae causes serious, life-threatening infections in humans in endemic and epidemic settings. the objective of this study was to determine whether the incidence of k. pneumoniae bloodstream infection (bsi) was higher during warm months. | 2008 | 18260762 |
| concentration-dependency of beta-lactam-induced filament formation in gram-negative bacteria. | ceftazidime and cefotaxime are beta-lactam antibiotics with dose-related affinities for penicillin-binding protein (pbp)-3 and pbp-1. at low concentrations, these antibiotics inhibit pbp-3, leading to filament formation. filaments are long strands of non-dividing bacteria that contain enhanced quantities of endotoxin molecules. higher concentrations of ceftazidime or cefotaxime cause inhibition of pbp-1, resulting in rapid bacterial lysis, which is associated with low endotoxin release. in the p ... | 2008 | 18261128 |
| successful solid organ transplantation from septicemic cadaveric donors: case report. | systemic donor infections especially with gram-negative organisms are regarded as an absolute contraindication to cadaveric organ donation for transplantation. this is largely due to fear of transmitting the pathogenic organisms to the immunosuppressed recipient. however, due to the current shortage of organs available for transplantation, clinicians are faced with the option to use organs from infected donors. between 1996 to january 2006, we collected 44 solid organs. two out of nine donors ha ... | 2008 | 18261610 |
| humoral immune response to klebsiella spp. in new zealand sea lions (phocarctos hookeri) and the passive transfer of immunity to pups. | during the 2001-02 and 2002-03 breeding seasons, epizootics of klebsiella pneumoniae resulted in a dramatic increase of pup mortality in new zealand sea lions (phocarctos hookeri; nzsls) on enderby island (auckland islands). to estimate the prevalence of infection in the nzsl population, a serologic test was developed using a western blot and a polysaccharide antigen derived from a k. pneumoniae isolate from a nzsl pup. all archived serum samples collected between 1997 and 1998 and 2004 and 2005 ... | 2008 | 18263817 |
| il-22 mediates mucosal host defense against gram-negative bacterial pneumonia. | emerging evidence supports the concept that t helper type 17 (t(h)17) cells, in addition to mediating autoimmunity, have key roles in mucosal immunity against extracellular pathogens. interleukin-22 (il-22) and il-17a are both effector cytokines produced by the t(h)17 lineage, and both were crucial for maintaining local control of the gram-negative pulmonary pathogen, klebsiella pneumoniae. although both cytokines regulated cxc chemokines and granulocyte colony-stimulating factor production in t ... | 2008 | 18264110 |
| evaluation of pyelonephritis in culled indoor and outdoor high parity sows. | the present trial was conducted in hungary in neighboring large indoor and outdoor pig production units, belonging to the same breeding company. rejected kidneys from 201 (out of 241; 83.4%) outdoor, and 191 (out of 512, 37.3%) indoor high parity sows, with previous history of recidiving postparturient fever and excessive postparturient vulvovaginal discharge were gross pathologically bacteriologically, and histologically evaluated. all rejected kidneys revealed chronic pyelonephritis. in outdoo ... | 2008 | 18265757 |
| purification of a lectin from eugenia uniflora l. seeds and its potential antibacterial activity. | the aim of this work was to analyse the antimicrobial properties of a purified lectin from eugenia uniflora l. seeds. | 2008 | 18266644 |
| clinical experience of serious infections caused by enterobacteriaceae producing vim-1 metallo-beta-lactamase in a greek university hospital. | the dissemination of acquired metallo-beta-lactamases (mbls) in members of the family enterobacteriaceae is regarded as an emerging clinical threat. the clinical characteristics and outcomes of 17 cases of infection due to mbl-producing isolates were analyzed. | 2008 | 18269335 |
| emphysematous cystitis in a asymptomatic haemodialysis patient. | 2008 | 18275509 | |
| optimized dosage and frequency of cefozopran for patients with febrile neutropenia based on population pharmacokinetic and pharmacodynamic analysis. | to establish a cefozopran (a fourth-generation cephem) population pharmacokinetic model using patient data and use it to explore alternative dosage regimens that could optimize the currently used dosing regimen to achieve higher likelihood of pharmacodynamic exposure against pathogenic bacteria. | 2008 | 18276604 |
| investigation of binding of udp-galf and udp-[3-f]galf to udp-galactopyranose mutase by std-nmr spectroscopy, molecular dynamics, and corcema-st calculations. | udp-galactopyranose mutase (ugm) is the key enzyme involved in the biosynthesis of galf. udp-galp and udp-galf are two natural substrates of ugm. a protocol that combines the use of std-nmr spectroscopy, molecular modeling, and corcema-st calculations was applied to the investigation of the binding of udp-galf and its c3-fluorinated analogue to ugm from klebsiella pneumoniae. udp-galf and udp-[3-f]galf were bound to ugm in a manner similar to that of udp-galp. the interconversions of udp-galf an ... | 2008 | 18278916 |
| noma (cancrum oris) in human immunodeficiency virus infection and acquired immunodeficiency syndrome (hiv and aids): clinical experience in zimbabwe. | this retrospective study describes the clinical features and management of noma (cancrum oris) in patients with hiv and aids. | 2008 | 18280380 |
| endogenous endophthalmitis complicating klebsiella pneumoniae liver abscess in europe: case report. | to report the first european case of endogenous endophthalmitis secondary to a liver abscess due to klebsiella pneumoniae expressing maga gene. | 2008 | 17668150 |
| modeling 1,2-dichloroethane biodegradation by klebsiella oxytoca va 8391 immobilized on granulated activated carbon. | a mathematical model of the biodegradation of xenobiotics by microbial cells attached to particles of granulated activated carbon was developed. the model allowed the quantitative evaluation of different characteristics of the biofilm behavior: retarded microbial growth, increased concentration of immobilized cells compared to suspended cultures, potential cell detachment from the solid support and consequent independent growth of free cells. the applicability of the model was demonstrated for o ... | 2008 | 17690915 |
| visual deterioration after gram-negative sepsis in neutropenia. | 2008 | 17684741 | |
| effects of prevalent freshwater chemical contaminants on in vitro growth of escherichia coli and klebsiella pneumoniae. | many surface and ground waters in the continental us are contaminated with a variety of chemical pollutants, which are usually present in concentrations in the ppm and ppb range. the effects of these pollutants on coliform bacteria, which are prominent members of the aquatic flora, are poorly understood. using a microtiter plate assay, isolates of escherichia coli (from chicken intestine and fresh water), and an isolate of klebsiella pneumoniae (from bovine milk) were exposed to varying concentr ... | 2008 | 17681655 |
| presence of nitrogen fixing klebsiella pneumoniae in the gut of the formosan subterranean termite (coptotermes formosanus). | a gram-negative facultative anaerobic enteric bacterium, klebsiella pneumoniae was isolated from the hindgut of the formosan subterranean termite (fst). it was characterized using, fatty acid methyl ester (fame) analysis, biolog assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and biochemical studies. the role of this isolate seems to be nitrogen fixation because the termite's diet is nitrogen deficient and the isolate produced significant amounts of ammonia when it wa ... | 2008 | 17709245 |
| characterization and identification of virulent klebsiella oxytoca isolated from abalone (haliotis diversicolor supertexta) postlarvae with mass mortality in fujian, china. | an epidemic of mass mortality of abalone (haliotis diversicolor supertexta) postlarvae aged 40 days or less has existed across south coast of china since the second half of 2002. among 20 bacterial strains isolated from diseased abalone postlarvae on 2216e marine agar plates during an outbreak of postlarval disease in august 2005, a predominant strain (designated strain 20) was demonstrated to be virulent to postlarvae with an ld(50) value of 1.0x10(5) colony forming units (cfuml(-1)) on day 4, ... | 2008 | 17716687 |
| metabolic flux and robustness analysis of glycerol metabolism in klebsiella pneumoniae. | the knowledge of the mechanism of flux distribution will benefit understanding cell physiology and regulation of metabolism. in this study, the measured fluxes obtained under steady-state conditions were used to estimate intracellular fluxes and identify the robustness of branch points of the anaerobic glycerol metabolism in klebsiella pneumoniae for the production of 1,3-propanediol by metabolic flux analysis. the biomass concentration increased as nadh(2)/nad(+) decreased at low initial concen ... | 2008 | 17713793 |
| microbiological quality of indoor and outdoor swimming pools in greece: investigation of the antibiotic resistance of the bacterial isolates. | during 1997-2005, the microbiological quality and susceptibility of bacterial isolates of swimming pool waters were investigated. a total of 462 water samples were collected from three indoor swimming pools (a teaching pool, a competition public pool, a hydrotherapy pool) and two outdoor swimming pools (a hotel semi-public and a residential private pool) in northwestern greece. all water samples were analyzed for the presence of bacteria, protozoa and fungi and susceptibility tests were performe ... | 2008 | 17728184 |
| dark h2 fermentation from sucrose and xylose using h2-producing indigenous bacteria: feasibility and kinetic studies. | cellulosic materials are the major components in biomass feedstock used for bioenergy production. hydrolytic products of cellulosic substances consist primarily of hexose (e.g., glucose) and pentose (e.g., xylose). in this study, the efficiency of fermentative conversion of sucrose (representing hexose) and xylose into h2 was examined with seven h2-producing pure strains isolated from a high-rate h2-producing system in our recent work. the isolates were identified as clostridium butyricum (strai ... | 2008 | 17889245 |
| extended-spectrum beta-lactamases: will they be the new 'methicillin-resistant staphylococcus aureus'? | 2008 | 17760887 | |
| gram-negative pathogen klebsiella oxytoca is associated with spontaneous chronic otitis media in toll-like receptor 4-deficient c3h/hej mice. | this report confirms the presence of gram-negative klebsiella bacteria in the middle ear of the c3h/hej mouse by culture, polymerase chain reaction (pcr), and electron microscopy. identification of the bacterial pathogen supports the c3h/hej mouse as an excellent model for spontaneous chronic otitis media and its effects on the middle and inner ear. | 2008 | 17851949 |
| molecular identification of coliform bacteria isolated from drinking water reservoirs with traditional methods and the colilert-18 system. | the accuracy of a traditional method (lactose utilization with acid and gas production) for the detection of coliform bacteria and e. coli was tested in comparison with method iso 9308-1 (based on acid formation from lactose) and the colilert-18 system (detection of beta-galactosidase). a total of 345 isolates were identified after isolation from water samples using api 20e strips. the colilert-18 led to the highest number of positive findings (95% of the isolates were assigned to coliforms), wh ... | 2008 | 17870668 |
| evaluation of antimicrobial susceptibility for beta-lactams using the etest method against clinical isolates from 100 medical centers in japan (2006). | this antimicrobial resistance surveillance study was performed in 100 medical centers. susceptibility testing (etest; ab biodisk, solna, sweden) of 9152 strains including escherichia coli (991 strains), klebsiella spp. (1000 strains), enterobacter spp. (971 strains), citrobacter spp. (803 strains), indole-positive proteae spp. (834 strains), serratia spp. (902 strains), acinetobacter spp. (874 strains), pseudomonas aeruginosa (992 strains), oxacillin-susceptible staphylococcus aureus (984 strain ... | 2008 | 17931820 |
| sperm motility changes after coincubation with various uropathogenic microorganisms: an in vitro experimental study. | we investigated the relationship between semen motility and various uropathogenic microorganisms. | 2008 | 17934792 |
| evaluation of detection of extended-spectrum beta-lactamases among escherichia coli and klebsiella spp. isolates by vitek 2 ast-n029 compared to the agar dilution and disk diffusion methods. | a total of 123 clinical escherichia coli and klebsiella spp. isolates were included in the study in order to evaluate vitek 2 ast-no29 (nordic) card for detection of extended-spectrum beta-lactamases (esbl) and to compare the results with genotypic esbl verification. the results were also compared to alternative phenotypic methods, i.e. agar dilution and disk diffusion. the strains that were esbl-positive according to ast-n029 were further analysed with the esbl test card, vitek 2 ast-n041. usin ... | 2008 | 17934979 |
| in vitro and in vivo antibacterial activities of dc-159a, a new fluoroquinolone. | dc-159a is a new 8-methoxy fluoroquinolone that possesses a broad spectrum of antibacterial activity, with extended activity against gram-positive pathogens, especially streptococci and staphylococci from patients with community-acquired infections. dc-159a showed activity against streptococcus spp. (mic(90), 0.12 microg/ml) and inhibited the growth of 90% of levofloxacin-intermediate and -resistant strains at 1 microg/ml. the mic 90s of dc-159a against staphylococcus spp. were 0.5 microg/ml or ... | 2008 | 17938194 |
| clinical impact of a pcr assay for rapid identification of klebsiella pneumoniae in blood cultures. | the clinical impact of a rapid pcr identification assay for klebsiella pneumoniae in positive blood cultures was prospectively evaluated. multivariate analysis identified the rapid pcr assay as the only significant factor in decreasing the time lapse preceding the initiation of appropriate antimicrobial therapy (hazards ratio, 3.03; confidence interval, 1.62 to 5.68; p, 0.001). | 2008 | 17942663 |
| a 7-year national survey on bacterial resistance in bronchoalveolar lavage from patients hospitalized in argentina. | the purpose of this study is to undertake a nationwide survey on bacterial resistance in bronchoalveolar lavage (bal) from patients hospitalized in argentina. a 2-month point prevalence study was conducted twice yearly (april-may and october-november) from 1997 to 2003 by 36 argentinean centers. antimicrobial susceptibility data of the potential pathogens recovered from the bal (samples containing <1% of squamous epithelial cells and bacterial counts >or=10(4) cfu/ml) of inpatients (i.e., >or=48 ... | 2008 | 17888608 |
| intrauterine infection with klebsiella pneumoniae: report of a case and literature review. | we report a case of intrauterine klebsiella pneumoniae infection that resulted in premature rupture of membranes and fetal demise at 20 weeks' gestation in a pregnancy achieved by in vitro fertilization. postmortem findings included massive panlobar pneumonia, the presence of abundant gram-negative, rod-shaped bacteria within the pulmonary air spaces and the lumen of the gastrointestinal tract, and fetal lung and blood cultures positive for klebsiella pneumoniae. the placenta showed severe acute ... | 2008 | 17939747 |
| synthesis, characterization and antibacterial activity of cobalt(iii) complexes with pyridine-amide ligands. | the ligands 2-(n-(x-pyridyl)carbamoyl)pyridine (x=2, 3 or 4 for hl1-hl3, respectively) and 2,6-bis(n-(y-pyridyl)carbamoyl)pyridine (y=2, 3 or 4 for h2l4-h2l6, respectively) in their mono- and di-deprotonated forms have been used to synthesize kinetically stable cobalt(iii) compounds [co(l1-3)3] (1-3) and na[co(l4-6)2] (4-6), respectively. the co(iii) ion is in octahedral environment and is surrounded by three bidentate ligands in complexes 1-3 and two tridentate ligands in complexes 4-6. ligands ... | 2008 | 17959275 |
| complex molecular epidemiology of extended-spectrum beta-lactamases in klebsiella pneumoniae: a long-term perspective from a single institution in madrid. | to analyse all extended-spectrum-beta-lactamase (esbl)-producing klebsiella pneumoniae isolates recovered from 2001 to 2004 in our institution and to compare this period with that of 1989 to 2000. | 2008 | 17967851 |
| effect of eucalyptus essential oil on respiratory bacteria and viruses. | the activity of eucalyptus globulus essential oil was determined for 120 isolates of streptococcus pyogenes, 20 isolates of s. pneumoniae, 40 isolates of s. agalactiae, 20 isolates of staphylococcus aureus, 40 isolates of haemophilus influenzae, 30 isolates of h. parainfluenzae, 10 isolates of klebsiella pneumoniae, 10 isolates of stenotrophomonas maltophilia and two viruses, a strain of adenovirus and a strain of mumps virus, all obtained from clinical specimens of patients with respiratory tra ... | 2008 | 17972131 |
| prevalent phenotypes and antibiotic resistance in escherichia coli and klebsiella pneumoniae at an indian tertiary care hospital: plasmid-mediated cefoxitin resistance. | the beta-lactam antibiotics, in combination with aminoglycosides, are among the most widely prescribed antibiotics. however, because of extensive and unnecessary use, resistance to these drugs continues to increase. in recent years, resistance in the indian bacterial population has increased markedly, the majority showing complex mechanisms. due to increased transcontinental movement of the human population, it would be wise to know the prevalence and resistance complexity of these strains, well ... | 2008 | 17981482 |
| characterization of integrative and conjugative element icekp1-associated genomic heterogeneity in a klebsiella pneumoniae strain isolated from a primary liver abscess. | genomic heterogeneity has been shown to be associated with klebsiella pneumoniae strains causing pyogenic liver abscesses (pla) and metastatic infections. in order to explore the mechanism responsible for genomic heterogeneity in k. pneumoniae, we compared the complete genomic sequences of strains ntuh-k2044 and mgh78578. an approximately 76-kbp dna fragment located adjacent to an asparagine (asn) trna gene was present in ntuh-k2044 but not in mgh78578. this fragment could be divided into three ... | 2008 | 17981959 |
| detection of extended-spectrum beta-lactamase-producing klebsiella pneumoniae in effluents and sludge of a hospital sewage treatment plant. | to detect esbl (extended-spectrum beta-lactamase)-producing klebsiella pneumoniae present in the effluents and sludge of a hospital sewage treatment plant, evaluating the treatment plant's potential to remove these micro-organisms. | 2008 | 17983431 |
| inhibitory effect of gram-negative and gram-positive microorganisms against helicobacter pylori clinical isolates. | to determine the in vitro inhibitory effect of several gram-negative and gram-positive microorganisms against helicobacter pylori clinical isolates. | 2008 | 17965421 |
| xenorhabdus antibiotics: a comparative analysis and potential utility for controlling mastitis caused by bacteria. | the role of antibiotics produced by bacterial symbionts of entomopathogenic nematodes is to suppress growth of microbes in the soil environment. these antibiotics are active against gram-positive and gram-negative bacteria, and were tested against mastitis isolates from dairy cows. | 2008 | 17976177 |
| use of tigecycline for the treatment of prolonged bacteremia due to a multiresistant vim-1 and shv-12 beta--lactamase-producing klebsiella pneumoniae epidemic clone. | we report the use of tigecycline, firstly with colistin and finally alone, in a patient with a persistent breakthrough bacteremia due to a klebsiella pneumoniae isolate harboring a metallo-beta-lactamase (vim-1) and an extended-spectrum beta-lactamase (shv-12). time-kill studies demonstrated that the combination of both compounds was synergistic along the first 12 h, suppressing the regrowth observed after 3 to 6 h when colistin was tested alone. | 2008 | 17996417 |
| a new mathematical model to evaluate simazine removal in three different immobilized-biomass reactors. | a new mathematical model based on the cinetical langmuir equation is developed to interpret and predict the effectiveness of simazine (sz) removal in immobilized-biomass reactor (ibr), to consider herbicide-support affinity (cx), and herbicide-cell affinity (cy). three solid supports: sepiolite monolith, granular sepiolite, and alginate were used in pilot-scale reactors that were inoculated with klebsiella planticola dsz. the abiotic process was analysed by measuring the sz sorption capacity of ... | 2008 | 17996921 |
| selection of shv extended-spectrum-beta-lactamase-dependent cefotaxime and ceftazidime resistance in klebsiella pneumoniae requires a plasmid-borne blashv gene. | in klebsiella pneumoniae, it is common for plasmid-located and chromosome-located bla(shv) copies to coexist within single cells. the plasmid-borne genes are mainly derived from two separate is26-mediated mobilizations of bla(shv). the objective of this study was to test the hypothesis that the presence of a non-extended-spectrum beta-lactamase (non-esbl) encoding plasmid-borne form of bla(shv) facilitates the cefotaxime (ctx)-mediated selection of esbl-expressing mutants, even when there is a c ... | 2008 | 17999968 |
| ongoing epidemic of blavim-1-positive klebsiella pneumoniae in athens, greece: a prospective survey. | to determine the current frequency and study the characteristics of vim-1-producing klebsiella pneumoniae isolates from bloodstream infections in greek hospitals. | 2008 | 17999975 |
| natural attenuation, biostimulation, and bioaugmentation in 4-chloroaniline-contaminated soil. | bioremediation treatments including natural attenuation (na), biostimulation (bs), and bioaugmentation (ba) were performed and compared regarding the degradation of 4-chloroaniline (4ca) contaminating two types of agricultural soil collected from nakornnayok (nn) and chiangmai (cm) provinces, thailand. despite the different soil properties, both soil types exhibited intrinsic potential for biodegradation. 4ca degradation by na in loam soil-nn was fairly effective (ca. 40%), while in sandy-clay l ... | 2008 | 18000705 |
| enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in klebsiella oxytoca. | klebsiella oxytoca mutants were isolated from the wild type strain me-303 after mutagenesis with uv coupled with diethyl sulfate and the following modified proton suicide method. by analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, me-ud-3, was isolated that produced 7.8% more 2,3-butanediol than me-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% an ... | 2008 | 18008166 |
| bioflocculant production by culture of serratia ficaria and its application in wastewater treatment. | a bioflocculant-producing bacterium was isolated from soil and identified as serratia ficaria. using optimized culture conditions a flocculating activity of 95.4% was obtained. it was found to be effective for flocculation of a kaolin suspension over weakly acidic ph (5-7); divalent cations (ca2+ and mg2+) enhanced the flocculating activity, while the co-presence of al3+ and fe3+ resulted the negative effect. measurements of zeta potential revealed that charge neutralization played an important ... | 2008 | 18024024 |
| antibiotic-resistant klebsiella pneumoniae in spain: analyses of 718 invasive isolates from 35 hospitals and report of one outbreak caused by an shv-12-producing strain. | 2008 | 18024490 |