Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [the diagnostic value of a culture study in detecting patients with trichomoniasis]. | the advantages of culture method for the diagnosis of trichomoniasis over microscopy of stained and native preparations are demonstrated. new liquid nutrient media for trichomonas cultivation reveal vaginal trichomonas in 99.48% of cases in medium, in 100% of cases in medium, and in 97.78% of cases. these media are universal and may be used at bacteriologic laboratories of treatment-and-prophylaxis institutions engaged in culture diagnosis of trichomoniasis. | 1989 | 2609750 |
| [synthesis of 2-aryl benzothiazoles and research on its antiparasitic activity]. | several 2-aryl benzothiazoles (with phenyl, naphthyl, thienyl and some oxygenated heterocycles) were synthesized. the anti-parasitic properties of these compounds were evaluated, in vitro and in vivo, against one nematode nippostrongylus brasiliensis and, in vitro against two protozoaires entamoeba histolytica and trichomonas vaginalis. only two compounds exhibited, in vitro a low nematicidal activity. | 1989 | 2610470 |
| trichomonas vaginalis infection in a lesbian. | 1989 | 2613220 | |
| prevalence of common infective agents of vaginitis. | a total of 100 women with vaginitis and 50 age matched healthy women were studied for common infective agents of vaginitis. the vaginal secretions collected from these women were examined for trichomonas vaginalis, g. vaginalis, and candida species. the prevalence of infective agents in the patients was t. vaginalis 11 per cent. candida species 30 per cent and gardnerella vaginalis 31 per cent. in contrast, in the control group the prevalence was candida species 14 per cent and g. vaginalis 22 p ... | 1989 | 2614050 |
| study of candida and trichomonas vaginalis in leucorrhoea. | of 150 clinically suspected cases of leucorrhoea, 44 cases (29.33%) showed growth of candida and 30 cases (20%) of trichomonas, by wet preparation. among the candida species the most frequent isolate was c albicans (56.8%), followed by c tropicalis (20.4%), c krusei (11.4%), c stellatoidea (9.1%) and c parapsilosis (2.3%). the higher incidence was found among the pregnant women than among non-pregnant women. among pregnant women multipara in third trimester showed higher incidence of candida and ... | 1989 | 2621360 |
| glycogen medium, antitrichomonal drug activity in vaginal liquids. | the amount of glycogen in vaginal liquids decreases with trichomonas vaginalis and this is connected with t. vaginalis activity in specimens. a glycogen-hydrolysed casein medium ("glycogen medium") added to vaginal liquid is a valid maintenance medium for t. vaginalis and therefore enables a direct test for antitrichomonas drugs to be performed. | 1989 | 2637795 |
| evaluation and management of vaginitis. an update for primary care practitioners. | there are three major causes of vaginitis symptoms that primary care practitioners should be able to diagnose and treat expertly. recent investigations have shown that bacterial vaginosis, the most common cause of vaginitis symptoms in patients not seen in sexually transmitted disease clinics, has a multifactorial etiology: gardnerella vaginalis is universally present but is not, per se, the etiologic agent. diagnosis and treatment are based, therefore, on evidence of a disturbed bacterial ecolo ... | 1989 | 2645843 |
| epidemiology of sexually transmitted diseases in rape victims. | rape is a violent crime that is increasing rapidly in incidence. victims can include male and female adults and children. these victims may be at risk for the acquisition of many sexually transmitted conditions. the risk will vary with the sex and socioeconomic status of the assailant as well as with the nature of the sexual contact. infections with neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, herpes simplex virus, human papillomavirus, and treponema pallidum have all bee ... | 1989 | 2649960 |
| [epidemiologic aspects of trichomoniasis]. | 1989 | 2655345 | |
| the emergency department diagnosis of trichomonas vaginitis. | trichomonas vaginalis is a common cause of vaginitis in emergency department patient populations. while the diagnosis of this condition is usually on the basis of wet-mount microscope slide preparations, other diagnostic technology exists. we studied the accuracy of the traditional wet-mount technique as compared with detection using direct immunofluorescence and culture techniques in ed patients with the presenting complaint of vaginal discharge. of 157 patients evaluated, the wet-mount techniq ... | 1989 | 2655509 |
| differentiation of gardnerella vaginalis, candida albicans, and trichomonas vaginalis infections of the vagina. | this study evaluated the positive predictive values of factors associated with gardnerella vaginalis, candida albicans, and trichomonas vaginalis for diagnosing vaginitis in a community-based population. one hundred ninety-six women with and without vaginal complaints were evaluated for historical factors, physical examination findings, and office laboratory results that were potentially associated with each of the three vaginal organisms. extensive microbiological tests were performed to detect ... | 1989 | 2656903 |
| carbohydrate, energy and hydrogenosomal metabolism of tritrichomonas foetus and trichomonas vaginalis. | 1989 | 2657037 | |
| trichomonas vaginalis. | although not life-threatening, trichomoniasis is a major health problem, especially in women. it is transmitted sexually and has a very high incidence worldwide. we argue that trichomonas vaginalis is not a commensal. attempts should be made to identify this pathogen in all suspicious cases. although culturing remains the most sensitive method to detect t vaginalis, newer methodologies such as monoclonal antibody fluorescent and direct staining make it possible to diagnose an infection with high ... | 1989 | 2668827 |
| vaginitis/vaginosis. | vaginitis/vaginosis is an extremely common medical problem. most cases are caused by yeast (predominantly candida albicans), the protozoan trichomonas vaginalis, or a specific mixture of bacteria (bacterial vaginosis). the prevalence of each of these varies with the patient population. the clinical signs used in differentiating among these and the associated bacterial flora are shown in table 3. because vaginitis/vaginosis cannot be adequately diagnosed solely on the basis of symptoms or physica ... | 1989 | 2676321 |
| trichomonas vaginalis. | trichomonas vaginalis is one of the most common sexually transmitted diseases. the epidemiology, diagnosis, treatment, and concerns about transmission are discussed. | 1989 | 2687745 |
| [retrospective study of mycotic and other colpitis pathogens]. | we had 170 female patients with presenting symptoms of vaginal itching, dysuria, dyspareunia, burning and vaginal discharge. we performed 200 times vaginal microbial exploration on the said 170 patients, with the repetition of the procedure on 30 out of these 200 patients for the second time. we had a positive result for vulvovaginal candidosis on 60 occasions. these positive results for yeast infection can be related to the incidence of other microbial infections such as mycoplasma-, ureaplasma ... | 1989 | 2688322 |
| [use of native microscopic examination of the vaginal flora in the diagnosis of trichomoniasis]. | microscopic diagnosis of vaginal discharge established so far by means of microbial vaginal pictures (mvp) has proved to be insufficient. phase contrast microscopy has an irreplaceable value in diagnosing vaginal infections transmitted by sexual intercourse. it yields quick and exact diagnosis of the disease, thus providing the possibility to administer treatment addressly and immediately. in equivocal findings and relapses vaginal cultivation is indispensable. in a series of 600 examined women ... | 1989 | 2691022 |
| bacterial vaginosis. | bacterial vaginosis is a common, non-inflammatory infection of the vagina. it is characterised by the presence of a thin, homogenous, greyish-white discharge. the differential diagnosis includes infection with trichomonas vaginalis and candida albicans. a diagnosis of bacterial vaginosis may be made by the detection of three of the following: characteristic discharge, ph of 5 or greater, clue cells and a positive koh amine test. culture of vaginal discharge is not necessary to effect a diagnosis ... | 1989 | 2694618 |
| new derivatives of 5-nitroimidazole: synthesis and antiparasitic activity. | the synthesis and the antiparasitic evaluation of twelve new 5-nitroimidaole derivatives has been carried out. the most effective compounds were the less hydrophilic pyridinium and imidazolium salts (iv), (v) and (x), and above all the tetrahydropyridine derivatives (xii) and (xiii). | 1989 | 2701965 |
| evaluation of proposed cytomorphologic criteria for the diagnosis of chlamydia trachomatis in papanicolaou smears. | to evaluate the proposed cytomorphologic criteria for the cervical cytologic diagnosis of chlamydia trachomatis infection, a study was made of 171 endocervical smears. all cytomorphologic elements that could be ascribed to chlamydia trachomatis infection were correlated with the diagnostic confirmation of this microorganism by monoclonal antibody (mab) staining. the presence of chlamydia trachomatis was detected in 21 samples (12.28%) by mab staining. comparing the cytomorphologic results with t ... | 1989 | 2728785 |
| association of chlamydia trachomatis and mycoplasma hominis with intrauterine growth retardation and preterm delivery. the john hopkins study of cervicitis and adverse pregnancy outcome. | the authors conducted a prospective study of risk factors for intrauterine growth retardation (birth weight less than a standard (race- and sex-adjusted) 10th percentile for gestational age) and preterm birth (birth prior to 37 weeks gestation) in a high-risk, inner-city, obstetric population, with special interest in pathogens colonizing the maternal genital tract. a total of 801 women within 22 to 30 weeks of gestation were enrolled and interviewed. subjects were cultured for gardnerella vagin ... | 1989 | 2729260 |
| infection with trichomonas vaginalis in a black population. | trichomonas vaginalis infection (tvi) was found by examination of pap smears in 25% of 3,005 unselected urban black women being screened for cancer of the cervix and vagina. the incidence was 22% in the group under 29 years of age; 69% in those between 30 and 59; and 9% in those over 60. women who had had a hysterectomy had 16% lower incidence of tvi than did the controls. class 1 reports (atypia, metaplasia, hyperplasia) were obtained in 10% of the entire group. cytologic changes were present i ... | 1989 | 2746693 |
| diagnostic procedures in vaginitis. | ninety-seven vaginitis patients underwent a standardized direct method of examination. bacterial vaginosis was found in 38.1%, candida albicans in 24.7% and trichomonas vaginalis in 13.4%. low secretional ph values were never met in bacterial vaginosis and t. vaginalis cases. a fishy odor, present in bacterial vaginosis, was also found in almost half of the t. vaginalis cases. endocervical bacteriological cultures are probably insufficient with respect to the detection of c. albicans and gardner ... | 1989 | 2759324 |
| differences in biologic maturation, sexual behavior, and sexually transmitted disease between adolescents with and without cervical intraepithelial neoplasia. | because several cofactors may influence the development of cervical intraepithelial neoplasia (cin) in young women, we compared differences in behaviors (sexual activity, contraception, and cigarette use), sexually transmitted disease (std) infection rates (chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, and herpes simplex virus), and cervical maturation (age of menarche and percentage of cervical ectopy) in groups of sexually active female adolescents with and without cin. ... | 1989 | 2769512 |
| clinical manifestations of vaginal trichomoniasis. | trichomonas vaginalis was detected by culture or wet-mount examination in 118 (15%) of 779 randomly selected women attending a sexually transmitted disease clinic. vaginal trichomoniasis was significantly associated with symptoms of yellow discharge, abnormal vaginal odor, and vulvar itching and with signs of colpitis macularis ("strawberry cervix"), purulent vaginal discharge, and vaginal and vulvar erythema. a logistic regression model was used to adjust for coinfections, as well as for demogr ... | 1989 | 2783346 |
| scanning electron microscopy of trichomonas vaginalis. | by scanning electron microscopy (sem), t. vaginalis surface appeared pleated with crater-like depressions. all the flagella appeared to start off in a single bundle from a shallow depression with a prominent rim. the side carrying the undulating membrane is extensively ruffled having a mulberry-like appearance to which flocculent material was frequently adherent. t. vaginalis nutrition appeared to proceed through pseudopodia-like extensions and phagocytosis by a constant deep invagination at the ... | 1989 | 2785150 |
| relatedness of structures of a major immunogen in trichomonas vaginalis isolates. | solubilization of live trichomonas vaginalis organisms with detergent caused the release of cysteine proteinases in the detergent extract which were inhibitable with n-alpha-p-tosyl-l-lysine chloromethyl ketone. the detergent extracts of all isolates tested possessed similar cysteine proteinase activities. these parasite proteinases rapidly degraded a prominent immunogen whose surface disposition undergoes phenotypic variation in some isolates. the relatedness of the forms of this immunogen amon ... | 1989 | 2785964 |
| [observation of the processes of phagocytosis and digestion of staphylococcus aureus by trichomonas vaginalis using a transmission electron microscope]. | trichomonas vaginalis was incubated with bacteria for 30 min. after centrifugation of staphylococcus aureus, incubation of t. vaginalis was continued for 40 min., 120 min. and 6 hrs. it was found that bacteria have been intensively phagocytated through whole surface of protozoan. phagosome with bacteria at different stages of degradation, up to defecation of undigested residues were observed. it was observed that some bacteria, apparently not susceptible to digestion, were immersed directly in c ... | 1989 | 2786283 |
| comparison of diamond's medium modified and kupferberg medium for detection of trichomonas vaginalis. | diamond's medium modified, the commercially available version of the klaas modification of diamond's medium, was compared to kupferberg medium and to direct wet-mount examination for detection of trichomonads in symptomatic patients. diamond's medium was found to be superior to both kupferberg medium (p less than 10(-4) and wet mount (p less than 10(-6]. | 1989 | 2787332 |
| secretory hydrolases of trichomonas vaginalis. | supernatants of three trichomonas vaginalis strains obtained from culture or after contact and cultivation with wish cells were assayed to characterize the nature of the factor/s responsible for the cytotoxic activity on wish cell monolayers. this factor, mainly secreted from the live, exponentially grown parasite, with a molecular weight higher than 10,000 daltons and heat-unstable, does not appear to be a protease. in its place, we demonstrated that the presence of some hydrolases (particularl ... | 1989 | 2787467 |
| survival of trichomonas vaginalis in human semen. | although exposure of trichomonas vaginalis to human semen is of short duration, any effect that this fluid may have on the urogenital protozoon could affect its transmission, especially if only few trichomonads are present. small numbers of parasites (about 2500/ml semen) incubated in semen from different donors at 37 degrees c, were found to survive or grow for up to 12 hours in all samples and for up to 24 hours in most. survival and growth of t vaginalis in semen most resembled that found in ... | 1989 | 2787774 |
| evaluation of six media for the growth of trichomonas vaginalis from vaginal secretions. | many media have been formulated for the growth of trichomonas vaginalis, but the relative sensitivities of these media have not been determined. we evaluated the ability of six media, including all five media commercially available in the united states, to grow trichomonas vaginalis from vaginal secretions. in a first experiment, we evaluated the ability of five media to grow t. vaginalis from vaginal secretions of 375 women and determined the optimal days on which to read culture tubes, by inoc ... | 1989 | 2787804 |
| trichomonas vaginalis and tritrichomonas foetus secrete neuraminidase into the culture medium. | supernatants taken from axenic cultures of trichomonas vaginalis and tritrichomonas foetus contain a neuraminidase activity, the detection of which is augmented when the trichomonad culture media are supplemented with 30% supernatant of confluent epithelial cultures. the enzyme was active against human erythrocytes, which became highly reactive to peanut agglutinin lectin. the specificity of the enzyme was checked by using a substrate specific to neuraminidase: 2'-(4-methylumbelliferyl)-alpha-d- ... | 1989 | 2788244 |
| characterization of a secreted cytoactive factor from trichomonas vaginalis. | trichomonas vaginalis, grown in dulbecco's modified eagle's medium with or without serum, produced a factor (tvf) which altered the morphology of certain mammalian cells in vitro. tvf had a mr of approximately 250 kda by gel filtration, approximately 50 kda by sds-page, and was heat (56 degrees c, 30 min) and ph (greater than 6 or less than 8) labile. co-incubation of tvf with adherent target cells caused a marked rounding and clumping of bhk-21 or cho-k1 cells, but had no effect on rk-13 or weh ... | 1989 | 2788372 |
| isolation of a cell-detaching factor of trichomonas vaginalis. | the pathogenetic role of soluble products of trichomonas vaginalis growth in culture is controversial. to evaluate this role, t. vaginalis was grown in broth and cell culture and the cell-free filtrate was applied to fresh cell culture monolayers. when adjusted to ph 6.5, filtrates obtained from 22-h culture growth totally disrupted mccoy, hep-2, human foreskin fibroblast, and chinese hamster ovary cell monolayers within 6 h. these detached cells remained greater than 90% viable. this cell-detac ... | 1989 | 2788661 |
| trichomonas vaginalis surface proteinase activity is necessary for parasite adherence to epithelial cells. | the role of cysteine proteinases in adherence of trichomonas vaginalis nyh 286 to hela and human vaginal epithelial cells was evaluated. only pretreatment of trichomonads, but not epithelial cells, with n-alpha-p-tosyl-l-lysine chloromethyl ketone (tlck), an inhibitor of trichomonad cysteine proteinases, greatly diminished the ability of t. vaginalis to recognize and bind to epithelial cells. leupeptin and l-1-tosylamide-2-phenylethyl chloromethyl ketone, other cysteine proteinase inhibitors, al ... | 1989 | 2789190 |
| identification of virus-like particles in eimeria stiedae. | when nucleic acid samples purified from sporozoites of eimeria stiedae were analyzed by agarose gel electrophoresis, an ethidium-stainable band with an apparent electrophoretic mobility of 6.5 kb was consistently observed. the band was readily degradable upon rnase treatment, and its susceptibility towards ribonuclease a on a decreasing ionic strength was suggestive of double-stranded rna (dsrna). electron microscopy revealed spherical, probably icosahedral, virus-like particles (vlp) with a dia ... | 1989 | 2797059 |
| [the microbial flora of the vagina in trichomoniasis]. | the vaginal flora was investigated in women with trichomoniasis by means of a culture method. trichomonas, yeast-like fungi and various types of bacteria were isolated. the applied microbiological technique allowed isolation not only of aerobic and cicrophilic bacteria but only of anaerobic bacteria. anaerobic bacteria were nonspore-forming and were isolated very frequently in the vagina. | 1989 | 2802080 |
| failure of mebendazole to cure trichomonal vaginitis resistant to metronidazole: case reports. | after a recent report showing the in vitro susceptibility to mebendazole of a strain of trichomonas vaginalis that was resistant to metronidazole, we present two cases of metronidazole resistant infection, both of which failed to respond to oral mebendazole. | 1989 | 2807288 |
| sexually transmitted pathogens in pregnant women in a rural south african community. | one hundred and ninety three consecutive pregnant women attending peripheral antenatal clinics attached to ngwelezana hospital, empangeni, kwa-zulu, were examined for evidence of sexually transmitted pathogens. the following incidences were found: trichomonas vaginalis 49.2% (95), candida spp 38.3% (74), chlamydia trachomatis 11.4% (22), gardnerella vaginalis 6.2% (12), neisseria gonorrhoeae 5.7% (11), positive syphilis serology results 11.9% (23), hepatitis b surface antigen 4.1% (eight). no wo ... | 1989 | 2807289 |
| chlamydia trachomatis cervical infection and oral contraceptive use among adolescent girls. | this study examines the relationship between oral contraceptive use and chlamydia trachomatis cervical infection in women less than 19 years of age. the clinical and epidemiologic data of 73 (19.4% prevalence rate) girls with chlamydial infection were analyzed, with special attention given to contraceptive use. the findings were compared with data from 303 girls who were chlamydia negative to assess the relationship between oral contraceptive use and c. trachomatis infection. use of an oral cont ... | 1989 | 2808080 |
| characterization and purification of extracellular proteases of trichomonas vaginalis. | extracellular protease activity was detected in serum-free culture filtrates of trichomonas vaginalis. the activity was demonstrated by hydrolysis of hide powder azure and possessed the characteristics of cysteine type proteases: inhibition by n-ethyl maleimide, cu2+, antipain, n-tosyl-l-phenylalanine chloromethyl ketone, n-tosyl-l-lysine chloromethyl ketone, leupeptin, chymostatin, and iodoacetamide, and enhancement by cysteine, edta, and dithiothreitol. the activity was optimal at acid ph and ... | 1989 | 2819601 |
| trichomonas infections in men. | trichomonas vaginalis, a common pathogen in the female genital tract, produces a characteristic clinical picture in women. less well recognized are the manifestations of trichomonas infestations of the male genital tract, which include urethritis and chronic prostatitis. multiple-glass urinalysis and selective use of trichomonas cultures may improve recognition of this organism in the family practice setting. | 1989 | 2916396 |
| commentary: family planning and sex education in std. | the incidence of sexually transmitted diseases (std) has risen in the past years to epidemic proportions even though medical understanding of stds has considerably improved. the most common forms of contraception (e.g. condoms, diaphragms, vaginal spermicides, intrauterine devices, and oral contraception pills) vary in how effective each is in preventing stds. condoms offer almost complete protection against many stds, including, gonorrhoea, syphilis, herpes virus, trichomonas, candidiases, ch ... | 1989 | 12283075 |
| prevalence of lower genital tract infections in pregnancy. | a descriptive profile of 106 pregnant women with various forms of cervicitis and vaginitis is provided. fifty women attending individual physicians' private offices are compared with 56 clinic patients: 34 attending a university prenatal teaching clinic and 22 attending a pregnancy termination unit. univariate analysis showed that single women were significantly more likely to be infected by mycoplasmas, yeasts, trichomonads, and gardnerella vaginalis than were married women. teenagers were more ... | 1988 | 3128883 |
| [pathogen spectrum of non-gonorrhea urethritis]. | by cultural investigations in 333 males with non-gonorrhoical urethritis (ngu) and 158 control probands it was demonstrated that chlamydia trachomatis is the main germ of this disease also in the gdr with a frequency of 40% of the cases. a chlamydia-negative ngu was caused in 15% by ureaplasma urealyticum in a number of greater than or equal to 10(4) germs/ml urine and in 5% by trichomonas vaginalis, respectively. candida albicans occurred significant more frequently in the patient group (21%) t ... | 1988 | 3043969 |
| new rapid latex agglutination test for diagnosing trichomonas vaginalis infection. | a newly developed latex agglutination test for trichomonas vaginalis infection was compared for sensitivity, specificity, efficiency, and positive and negative predictive values with microscopy, culture, and an enzyme linked immunosorbent assay (elisa) in the diagnosis of 395 women attending a genitourinary medicine clinic. t vaginalis infection was diagnosed in 42 (11%) women. the sensitivities of both the latex agglutination test and the elisa were 95% compared with 74% for microscopy and 76% ... | 1988 | 3045161 |
| analysis by immunofluorescence of alpha 1-antitrypsin levels on the surfaces of trichomonas vaginalis strains differing in virulence. | 1988 | 3045793 | |
| treatment failure in trichomonas vaginalis vaginitis. | 1988 | 3049301 | |
| alternating phenotypic expression of two classes of trichomonas vaginalis surface markers. | the antigenic heterogeneity of trichomonas vaginalis is due in part to the membrane disposition of immunogens (repertoire a) among some but not all isolates and among subpopulations of trichomonads of certain isolates. heterogeneous t. vaginalis isolates undergo phenotypic variation for the a repertoire of immunogens. the presence of immunogens on (a+ phenotype) or the absence of them from (a- phenotype) trichomonal surfaces clearly influences the virulence traits of the pathogenic human trichom ... | 1988 | 3055209 |
| [comparative study of the susceptibility of candida albicans and trichomonas vaginalis, on some products used as larvicides, trichomonacides and/or bactericides]. | 1988 | 3066257 | |
| localization of acetylated alpha-tubulin in tritrichomonas foetus and trichomonas vaginalis. | we used monoclonal antibodies specific for acetylated and nonacetylated alpha-tubulin to detect and to localize microtubules containing acetylated alpha-tubulin (stable microtubules) in the pathogenic protozoa tritrichomonas foetus and trichomonas vaginalis. sds-page analysis showed that tubulin is a major protein of both parasites, being enriched in cytoskeletal preparations of whole cells extracted with triton x-100. the monoclonal antibodies, which recognize all isoforms of alpha-tubulin (b-5 ... | 1988 | 3066505 |
| antiparasitic activity of nine pyrazole derivatives against trichomonas vaginalis, entamoeba invadens and plasmodium berghei. | nine nitropyrazole derivatives were prepared and tested against trichomonas vaginalis in vitro and in vivo, entamoeba invadens in vitro and plasmodium berghei in vivo. three of the compounds, 4-4-nitropyrazole, 1-methyl-4-nitropyrazole and 4,4'-dinitro-1,1'-methylenedipyrazole, have an activity similar to that of metronidazole (used as the reference compound) against t. vaginalis and e. invadens after 48 hours of incubation. all the compounds tested were inactive against p. berghei. | 1988 | 3074738 |
| basic properties of tritrichomonas mobilensis hemagglutinin. | tritrichomonas mobilensis is a recently described enteric protozoon of squirrel monkeys. an earlier report identified one of the metabolic products of this organism as a lectinlike hemagglutinin. its further properties were determined in this study. culture supernatants of t. mobilensis fp4190 were concentrated by ultrafiltration through a membrane with 100,000-molecular-weight cutoff. high titers of agglutinin against human erythrocytes were obtained. incubation at 70 degrees c for 15 min resul ... | 1988 | 3170709 |
| the interaction of trichomonas vaginalis and tritrichomonas foetus with epithelial cells in vitro. | the madin-darby canine kidney epithelial cell line (mdck) was used as a model for trichomonad-host cell interaction. two laboratory strains of the human parasite trichomonas vaginalis and the cattle's parasite tritrichomonas foetus or their supernatants from axenic cultures were allowed to interact with confluent epithelial cultures. the interaction process studied by transmission and scanning electron microscopy revealed that both parasites adhere to monolayers through flagella, cell body and p ... | 1988 | 3180257 |
| effects of antimicrobial therapy on sperm-mucus interaction. | sperm-mucus interaction under in-vitro or in-vivo conditions might be affected by microorganisms colonizing the reproductive tract. in order to study the influence of antimicrobial therapy, an extensive microbial screening was performed including chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, neisseria gonorrhoeae, a broad spectrum of potentially pathogenic aerobic and anaerobic bacteria, trichomonas vaginalis, herpes simplex virus and yeasts. one-hundred-and-six couples with ... | 1988 | 3182976 |
| prostitution in sheffield: differences between prostitutes. | this study to assess differences between street walking prostitutes and sauna girls who attended this clinic in 1986 and 1987 showed that fewer street walkers used barrier methods for disease prevention with clients or accepted tests for antibody to human immunodeficiency virus (hiv) when offered and more street walkers practised their profession in both sheffield and london. they therefore represent a potential pathway for the heterosexual spread of hiv to the region. trichomonas vaginalis was ... | 1988 | 3224974 |
| giardia-bile salt interactions in vitro and in vivo. | giardia lamblia predominantly colonizes the proximal small intestine where bile is plentiful. we have investigated interactions between bile and this parasite by (i) examining the specificity of the stimulatory effect of bile on parasite growth in vitro, (ii) studying the possible association between giardiasis and bile salt deconjugation in vivo, and (iii) quantifying bile salt uptake by giardia and relating this to uptake by other microorganisms. our findings indicate that the growth promoting ... | 1988 | 3232181 |
| giardia duodenalis: enhanced growth in cell culture. | growth of giardia duodenalis in broth and in animals has been studied in considerable detail. in contrast, the kinetics of growth in cell culture have been little evaluated. in this study, in vitro growth of g. duodenalis was evaluated in cell culture, primarily using mouse mccoy cells in vials. the media used were giardia broth (tyi-g), trichomonas vaginalis broth (tyi-t), and standard cell culture media (cmga) alone and in combination (2 parts by volume cmga to one part of tyi broth). addition ... | 1988 | 3232182 |
| multiple populations of double-stranded rna in two virus-harbouring strains of trichomonas vaginalis. | the existence of six dsrna segments of trichomonas vaginalis virus was confirmed and the molar mass and relative abundance of these segments were determined by agarose gel electrophoresis with reovirus dsrna serving as a standard. the m's were 3.5, 3.4, 3.2, 2.5, 1.4 and 0.34 mg/mol for the two strains studied, the relative abundances, however, were 1.0, 1.4, 3.0, 0.3, 2.7, 4.2 and 1.0, 0.6, 1.7, 0.5, 3.4 1.0 for these strains, respectively. cell homogenate fractionation showed that all dsrna se ... | 1988 | 3240907 |
| [cyto-microbiologic study of cervico-vaginal specimens, associated with a serologic study to detect sexually transmissible diseases in madagascan prostitutes]. | a microbiological survey of cervical and vaginal smears associated with a serological survey of sexual diseases was carried out on 298 prostitutes at the four principal malagasy sea ports. this survey leads us to these conclusions: the great frequency of specific inflammations (72.1%) with trichomonas vaginalis in the first place (39.2%). the serological tests proved a high rate of positive treponemical serologies (25.2%), whose the half of the cases are evolutive forms. nevertheless prevalence ... | 1988 | 3242427 |
| identification and properties of trichomonas vaginalis proteins involved in cytadherence. | trichomonas vaginalis nyh286 surface proteins which are candidates for mediating parasite cytadherence (adhesins) were identified. at least four trichomonad protein ligands ranging in relative molecular mass from 65 to less than or equal to 21 kilodaltons were found to selectively bind to chemically stabilized hela cells. the proteins were present on the surfaces of 10 different isolates of t. vaginalis examined; however, the nonpathogenic trichomonad t. tenax did not possess similar hela cell-b ... | 1988 | 3257206 |
| the diagnosis of vaginal trichomoniasis. | 1988 | 3257533 | |
| the purification and properties of two soluble reduced nicotinamide: acceptor oxidoreductases from trichomonas vaginalis. | the occurrence of soluble reduced nicotinamide nucleotide:acceptor oxidoreductases has been reported in a number of strains of the oxygen-tolerant anaerobe trichomonas vaginalis and other trichomonad species. the quantitatively more important enzyme in most strains of t. vaginalis is an nadh oxidase which produces water from the reduction of oxygen. this enzyme has been purified by a combination of gel filtration, chromatofocusing, cibacron blue chromatography and high pressure gel permeation ch ... | 1988 | 3257811 |
| fewer trichomonas vaginalis organisms in vaginas of infected women during menstruation. | the aims of the present study were to count trichomonas vaginalis organisms recovered from the vaginas of patients with trichomoniasis, and to obtain data concerning changes in sizes of trichomonal populations during the menstrual cycle. in about 80% of symptomatic as well as symptomless patients, more than 1 x 10(5) parasites per ml could be obtained from vaginal washes. during menstruation, however, the number of trichomonads decreased appreciably, with subsequent increases within three to six ... | 1988 | 3257936 |
| in vitro susceptibility of trichomonas vaginalis to 50 antimicrobial agents. | we determined the susceptibilities of five strains of trichomonas vaginalis, one of which was metronidazole resistant, to 50 antimicrobial agents. for the metronidazole-susceptible strains, the most active agents were metronidazole, tinidazole, mebendazole, furazolidone, and anisomycin. against the resistant strain mebendazole, furazolidone, and anisomycin were the most active. antifungal agents, beta-lactams, macrolides, aminoglycosides, and folic acid antagonists were ineffective against all s ... | 1988 | 3258142 |
| use of a crude extract or a purified antigen of trichomonas vaginalis for the detection of secretory antibodies by elisa. | a crude aqueous extract (t2) or a gel-filtration purified antigen of t. vaginalis was utilized to detect secretory antibodies in cervico-vaginal secretions by elisa. the test was developed under optimum conditions using a rabbit anti-trichomonas serum. conditions established for monitoring antibodies to trichomonas in immunized rabbits were equally effective for human secretions. the crude extract was capable of assessing secretory antibodies in 45% of women with acute trichomoniasis and 43% of ... | 1988 | 3258398 |
| mouse spleen cell responses to trichomonal antigens in experimental trichomonas vaginalis infection. | subcutaneous inoculation of live t. vaginalis into mice caused splenomegaly, particularly when using strains of parasites with low pathogenicity. the proliferative responses of spleen cells from uninfected mice, as measured by [3h] tdr uptake, showed that trichomonal antigens, whether from strains with high or low pathogenicity, have no mitogenic activity. spleen cells from mice infected with trichomonads of low pathogenicity showed a proliferative response to trichomonal antigens that was maxim ... | 1988 | 3258637 |
| in vitro susceptibility of trichomonas vaginalis to metronidazole and treatment outcome in vaginal trichomoniasis. | we have identified trichomonas vaginalis strains resistant in vitro to metronidazole, especially under aerobic conditions. since little is known about the relationship of treatment outcome to metronidazole susceptibility of t. vaginalis, we studied the aerobic and anaerobic susceptibility to metronidazole of 310 clinical isolates of t. vaginalis. of 199 patients with known outcomes after metronidazole treatment for vaginal trichomoniasis, the geometric mean minimal lethal concentration (mlc) und ... | 1988 | 3258675 |
| determination of cytopathogenic effects of trichomonas vaginalis on cultured cells by assaying 51cr release. | by use of an assay for 51cr release, it was attempted to determine the cytopathogenic effects of trichomonas vaginalis on mammalian cultured cells. 51cr was released from na2(51)cro4-labeled hela, l-929, fl, vero and llc-mk 2 cells. hela cells were most sensitive to the damage, followed by l-929, fl, vero and llc-mk 2 cells. the effect depended upon the number of parasite and the incubation period. the maximum 51cr release from the labeled cells was observed at 37 degrees c and ph 6.0. these res ... | 1988 | 3259767 |
| flagella-bearing bare trichomonas vaginalis nuclei in papanicolaou smears. | 1988 | 3259922 | |
| [anthelmintic agents. 5. long-chain and hydroheterocyclic-substituted chloro-diallylamino-1,3,5-triazines]. | 1988 | 3260475 | |
| differential susceptibility of fresh trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus. | the ability of complement in human menstrual blood and cervical mucus to kill trichomonas vaginalis was compared with that of complement in serum, and 95 fresh trichomonal isolates obtained from vaginal wash material were evaluated for susceptibility to complement immediately after isolation. only serum and menstrual blood with haemolytic activity produced total cytolysis of t vaginalis. the cytolytic abilities of these fluids were totally inactivated by treatment with heat or edetic acid (edta) ... | 1988 | 3261708 |
| specific parasitism of purified vaginal epithelial cells by trichomonas vaginalis. | human vaginal epithelial cells (vecs) from vaginal swabs obtained from normal women or from patients with trichomoniasis were purified, and vec parasitism by trichomonas vaginalis was examined. trichomonads bound equally well to live or dead vecs, and up to 20% of vecs were parasitized. trichomonal cytadherence of human vecs was time, temperature, and ph dependent. saturation binding levels of live trichomonads to vecs gave approximately 2 organisms adherent to parasitized vec. no differences in ... | 1988 | 3262088 |
| fewer trichomonas vaginalis organisms in vaginas of infected women during menstruation. | 1988 | 3262565 | |
| [observation on trichomonas vaginalis by scanning electron microscopy]. | 1988 | 3263220 | |
| monoclonal-antibody-based enzyme-linked immunosorbent assay for trichomonas vaginalis. | trichomonas vaginalis is estimated to infect 4 million women per year in the united states. the diagnosis of trichomoniasis is predominantly achieved by direct microscopic examination of vaginal exudates. this subjective diagnostic procedure is reported to be 75% sensitive under ideal circumstances. we have developed an enzyme-linked immunosorbent assay (elisa) for the detection of t. vaginalis directly from vaginal exudates. the elisa employs a monoclonal antibody specific for a 65-kilodalton s ... | 1988 | 3263386 |
| demonstration of concanavalin a- and wheat germ agglutinin-binding sites on the trichomonas vaginalis surface coat using lectin-gold particle conjugates. | 1988 | 3263459 | |
| [morphology and biological activity of trichomonas vaginalis grown on various nutrient media]. | 1988 | 3264099 | |
| [ultrastructural analysis of the interrelation of the causative agents of urogenital infections with the cells of the macro-organism in syphilis patients]. | 1988 | 3264100 | |
| effects of antimicrobial agents on growth and chemotaxis of trichomonas vaginalis. | the motility of viable trichomonas vaginalis organisms is readily demonstrable in a clinical wet mount or cultured specimens. we attempted to determine whether migration is a dynamic process such that the organisms move to avoid exposure to toxic antimicrobial agents. with the use of axenic cultures of t. vaginalis that were radiolabeled and assayed for chemotaxis in plastic multiwelled plates with a membrane filter inserted to trap organisms, the response of clinical isolates to various antimic ... | 1988 | 3264130 |
| [verification of the value of the elisa test in the diagnosis of urogenital trichomoniasis]. | 1988 | 3264747 | |
| [use of the elisa method in the determination of antibodies to trichomonas vaginalis]. | 1988 | 3264749 | |
| [complement-binding antibodies in women simultaneously infected with various species of trichomonas]. | 1988 | 3265240 | |
| [intradermal test with trichomonas vaginalis allergen in patients with urogenital trichomonas infection]. | 1988 | 3265243 | |
| the effect of hormones on trichomonas vaginalis. | the hormonal milieu can alter susceptibility to infection. the effect of hormones on trichomonas vaginalis was studied utilizing axenically cultured clinical isolates. oestrogens, in physiological concentrations, decreased the growth of the organisms and their attachment to mammalian cells in vitro, and acted as a chemorepellent. the specificity of these effects was verified by their being blocked with anti-oestrogens, by the dose- and time-dependency of the responses, and by the lack of effect ... | 1988 | 3265429 |
| [a dried nutrient medium made from the fermented hydrolysates of a microorganism biomass for isolating trichomonas vaginalis]. | 1988 | 3265559 | |
| human amnion membrane as a model for studying the host-parasite relationship in trichomoniasis. | 1988 | 3265702 | |
| isoenzyme patterns of isolates of trichomonas vaginalis from vancouver. | isoenzyme patterns of 63 isolates of trichomonas vaginalis obtained in vancouver were evaluated by use of thin-layer starch-gel electrophoresis. we attempted to use eight enzymes, but only four gave reproducible and interpretable results. there were four patterns with malic enzyme, two with malate dehydrogenase, one with hexokinase, and four with lactate dehydrogenase. the isoenzyme patterns of the 63 isolates were classified into 15 groups, but 49 (78%) fell into five groups and 14 (22%) fell i ... | 1988 | 3265809 |
| [sensitivity of trichomonas vaginalis to desiccation]. | 1988 | 3266051 | |
| trichomonas vaginalis: preliminary characterization of a sperm motility inhibiting factor. | this study determined the effects of trichomonas vaginalis trophozoites, subcellular fractions, and medium from axenic t. vaginalis cultures on human sperm motility and viability. spent medium (ph 7.0) caused complete cessation of sperm motility after 15 minutes incubation. trophozoite soluble fraction or formalin-killed trophozoites caused a 50 percent reduction in sperm motility, compared to 25 percent reduction caused by the trophozoite particulate fraction or the sterile medium and three per ... | 1988 | 3266454 |
| effect of metronidazole on the cell surface charge of trichomonas vaginalis and tritrichomonas foetus. | 1. the effect of metronidazole, a drug used in the treatment of trichomoniasis, on the surface charge of both trichomonas vaginalis and tritichomonas foetus was analyzed by cell electrophoresis. 2. incubation of the parasites under anaerobic conditions for 2 or 120 min in the presence of 2.0 micrograms/ml metronidazole, a concentration which inhibits cell growth by about 50%, led to a marked decrease in the net surface charge of the parasites. 3. the metronidazole analogue, 1-hydroxyethyl-2-meth ... | 1988 | 3266844 |
| [trichomonas vaginalis, epithelial cells, spermatozoa, and immunologic reactions. how and why an infection persists unchanged despite knowing an adequate drug therapy]. | 1988 | 3267183 | |
| sexually transmitted diseases and sexual behavior in urban adolescent females attending a family planning clinic. | specimens from the lower genital tract of 102 sexually active urban adolescent females were tested for the common sexually transmitted diseases (stds). the results were correlated with concomitant clinical, demographic, and historic data. forty-one percent (42/102) were infected with one or more of the following organisms: chlamydia trachomatis (26/102), trichomonas vaginalis (13/102), neisseria gonorrhoeae (10/102), yeast (6/102), condyloma (5/102), and herpes simplex virus (1/44). mixed infect ... | 1988 | 3335474 |
| influence of gender in pathogenesis of trichomoniasis in congenitally athymic (nude) mice. | evaluation of abscesses appearing in male and female euthymic and athymic (nude) balb/c mice after subcutaneous injection of trichomonas vaginalis in the dorsal region showed that females were more susceptible than males. female euthymic mice, however, were more susceptible than male athymic mice, and splenectomised athymic males were more susceptible than non-splenectomised athymic males. f1 female athymic mice were the most susceptible, as their abscesses reached a peak size five days earlier ... | 1988 | 3346023 |
| [trichomonas vaginalis infection. frequency and diagnosis in women]. | 1988 | 3353949 | |
| association of coexisting vaginal infections and multiple abusers in female children with genital warts. | all 11 girls less than 12 years of age presenting to the pediatric clinic between 1980 and 1986 with genital warts were evaluated by the child protection team for possible sexual abuse. nine of the 11 girls had condylomata acuminata, and two had verruca vulgaris. ten of the 11 had historical and/or physical evidence other than the warts that confirmed sexual abuse. six girls had coexisting vaginal infections such as bacterial vaginosis and infections due to various pathogens including neisseria ... | 1988 | 3358240 |
| treatment failure in trichomonas vaginalis infections in females. i. concentrations of metronidazole in plasma and vaginal content during normal and high dosage. | concurrent estimation by high pressure liquid chromatography (hplc) of the concentrations of metronidazole in plasma and vaginal content in 12 patients with infections due to trichomonas vaginalis who did not respond to normal and high-dose treatment has shown that the two levels are closely related to each other and to the dose. individual idiosyncrasies in absorption of drug from the gut lumen or in its transfer into the vaginal content are unlikely to be the cause of treatment failure. | 1988 | 3360695 |
| trichomonal vaginitis: evaluation of serological tests and identification of immunoreactive surface peptides. | an indirect haemagglutination assay (iha) with polysaccharide and protein antigens of trichomonas vaginalis and an enzyme linked immunosorbent assay (elisa) were used to test for antibodies against t vaginalis in 58 women with trichonomal vaginitis and 48 with non-specific vaginitis. eleven antibody positive sera were used in a radioimmunoprecipitation assay (ripa) to identify surface peptides that elicited antibody responses in infected women. the serological tests were less sensitive than biol ... | 1988 | 3384427 |
| [metronidazole-resistant trichomoniasis and successful therapy following high dosage]. | a patient is reported who suffered for several months from a trichomonas vaginalis infection that was resistant to the usual low-dose treatment with 5-nitro-imidazole derivatives. following various ineffective therapeutic trials, the agent was isolated in order to determine its sensitivity to 5-nitro-imidazole. the resistance of the isolate to metronidazole was confirmed in vitro and in an animal experiment; the patient was therefore treated with high daily doses of metronidazole, 3 x 750 mg ora ... | 1988 | 3384665 |