Publications

an interstitial deletion of chromosome 7 may characterize a subgroup of uterine leiomyoma.		1988	3165693
vibrio vulnificus necrotizing fasciitis presenting with compartmental syndrome of the hand.	vibrio vulnificus necrotizing fasciitis is an unusual clinical problem. when it presented as a compartment syndrome, the picture was obscured. we report a rare case of such a presentation. a below-elbow amputation was performed and the patient recovered.	1988	3171309
coagglutination of vibrio cholerae, vibrio mimicus, and vibrio vulnificus with anti-flagellar monoclonal antibody.	monoclonal antibodies (mabs) with serological activity for purified flagellar (h) core protein prepared from vibrio cholerae were identified by enzyme-linked immunosorbent assay. four of these mabs reacted with the flagella of v. cholerae and v. mimicus exclusively, while eight mabs reacted with at least 1 of 30 heterologous vibrio species tested by enzyme-linked immunosorbent assay or coagglutination. it appears that v. cholerae and v. mimicus express similar, if not identical, h determinants u ...	1988	3182988
septicaemia caused by vibrio vulnificus.	vibrio vulnificus is an uncommon cause of septicaemia. a few reports suggest that patients with chronic liver disease are more susceptible. we report two cases of septicaemia caused by this organism and emphasise the need for early empirical therapy.	1988	3183404
vibrio vulnificus meningitis in a boy with thalassemia after eating raw oysters.		1988	3186359
vibrio vulnificus and pulmonary infection.		1988	3195881
the effect of alcoholic beverages on iron and zinc metabolism in the rat.	1. male wistar rats (approximately 200 g) were given distilled water and a semi-synthetic control diet for 6 d. on day 7, 37 kbq 65zn were administered intramuscularly and the rats were given distilled water, beer, cider, red wine, whisky or ethanol as their only source of fluid. the wine, whisky and ethanol were diluted so that each of the beverages contained a similar ethanol concentration (approximately 30 g/l). food and fluid intake, growth rate and whole-body 65zn were measured regularly ov ...	1988	3196669
in vivo electrochemical detection of 5-hydroxyindole within the trigeminal nucleus caudalis of freely moving rats: the effect of morphine.	the trigeminal nucleus caudalis is considered the equivalent of the orofacial nociceptive system of the dorsal horn of the spinal cord. at the level of this trigeminal area (i.e. medullary dorsal horn) the transmission of noxious inputs is strongly modulated by a descending, serotonergic system mainly originating from the nucleus raphe magnus (nrm). the present study in freely moving animals reports the effect of morphine on the 5-hydroxyindole oxidation current recorded in the medullary dorsal ...	1988	3196919
vibrio vulnificus infections.		1988	3197443
differential toxicity of cis and trans isomers of dichlorodiammineplatinum.	nephrotoxicity is the dose-limiting toxic effect of cis-dichlorodiammineplatinum (cis-platin) in humans. its stereoisomer transplatin does not have any toxicity at equimolar concentrations, and it also possesses little antitumor activity. in this study, subcellular localization of both the platinum isomers was examined in the liver and kidney of the mouse 24 hours following the drug administration. levels of the platinum isomers were measured using flameless atomic absorption. the results showed ...	1988	3199415
exploitation of bedding in dairy outdoor calf hutches by immature house and stable flies (diptera: muscidae).		1988	3204628
protomyobia (acari: myobiidae) species groups and the brevisetosa complex of mites on north american shrews (mammalia: soricidae).		1988	3204630
[clinical studies of rokitamycin dry syrup on skin and soft tissue infections in the pediatric fields].	rokitamycin (rkm) dry syrup, a newly developed macrolide antibiotic, was administered to children with ages between 6 months and 15 years and 10 months suffering from skin and soft tissue infections including 41 cases of impetigo, one case of staphylococcal scalded skin syndrome (ssss) and 2 cases of subcutaneous abscess totalling 44 cases. the average daily dose level used was 31.3 mg/kg divided into 3 or 4 doses, for an average of 6 days of treatment. mics of 4 different macrolide antibiotics ...	1988	3204657
acute hepatic failure precipitated in a patient with subclinical liver disease by vibrionic and clostridial septicemia.	a case of fatal septicemia caused by vibrio vulnificus and clostridium bifermentans in a patient with subclinical liver disease is described. the patient appeared to recover from the infection initially after antibiotic therapy but finally succumbed to rapidly developing hepatic failure. postmortem examination revealed hemochromatosis. the pathogenesis of the polymicrobial septicemia and hepatic failure is discussed in the light of the published literature.	1988	3211597
in vitro activity of sulbactam/ampicillin and ampicillin against methicillin-sensitive and methicillin-resistant staphylococcus aureus and staphylococcus epidermidis.	minimum inhibitory concentrations (mics) of ampicillin and ampicillin + sulbactam (1:1) against 165 strains of staphylococcus aureus and 72 strains of staphylococcus epidermidis have been evaluated. the activity of the combination was very good. a concentration of 16 micrograms/ml + 16 micrograms/ml inhibited 96.9% of s. aureus and the 100% of s. epidermidis strains (at the same concentration ampicillin alone inhibited only 55.15% and 56.9% of s. aureus and s. epidermidis strains respectively). ...	1988	3224398
in vitro effect of xipamide on sodium-potassium transport systems in human erythrocytes.	the in vitro effects of xipamide in a concentration range of 10(-8) to 10(-2) m were investigated on various na+ and k+ transport systems in human red blood cells. xipamide inhibited the anion carrier or dids-sensitive lico3- -influx starting from a concentration of 10(-5) m. however, a decrease in the na+, k+-pump and the na+, k+-cotransport activity and a rise in the passive permeability of the cell membrane was only observed starting from a concentration of 10(-4) m xipamide.	1988	3226221
excision of cutaneous tumors in the horse using histologic guidance.	a highly successful surgical technique for removing cutaneous tumors in humans was used in seven horses with cutaneous squamous cell carcinoma (n = 3) or sarcoid (n = 4). in humans, the complete underside of the tumor is evaluated histologically by processing horizontal sections from the tumor base, and orientation between the wound surface and the undersurface of the excised tumor is maintained by mapping both surfaces. the technique ensures that small foci of residual tumor can be located accu ...	1988	3227634
cytolytic toxins of vibrio vulnificus and vibrio damsela.		1988	3231100
[epidemiology and developmental aspects of trypanosomiasis in the source of the sangha, congo].		1988	3233035
[kidney morphology in hemolytic-uremic syndrome].	morphology of the kidneys in hemolytic-uremic syndrome is considered basing on autopsy findings obtained for 3 infants with 5-17-day history of acute renal failure. a newborn infant of 17 days developed the disease after feto-fetal hemotransfusion when macerated fetus-donor hemolysis products entered the circulation of the fetus-recipient through monochorionic placenta. the second case in an infant of 6 months was due to adtp vaccine. the last infant aged 16 months manifested the syndrome in the ...	1988	3233069
[effectiveness of distal gene translation in polycistrons depends upon the arrangement of regulatory signals on a template].	the role of the translational terminator and initiator signals arrangement for two adjacent genes in polycistronic mrna has been studied. semisynthetic beta-galactosidase gene (lacz) of e. coli and fragment of phage m13 dna (with promoter pviii, gene ix, and part of gene viii) were used for constructing of the ix-viii-lacz artificial polycistronic operon. cloning of the constructs into pbr322 vector resulted in a number of plz381n plasmids differing by the mutual arrangement of gene viii transla ...	1988	3233097
on the time-dependent diffusion of macromolecules through transient open junctions and their subendothelial spread. i. short-time model for cleft exit region.	in this two-part study we shall quantitatively study, using time-dependent models, the hypothesis that transient open junctions associated with widely scattered endothelial cells undergoing mitosis are the structural equivalent for the large pore pathway via which macromolecules the size of albumin or larger cross the vascular endothelium. in an earlier steady-state model [am. j. physiol. 248, h945-960 (1985)], the authors demonstrated that such an open-junction pathway could quantitatively acco ...	1988	3256708
antibody synthesis specific for nonoral antigens in inflamed gingiva.	in vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal b-cell activators (pba). we reasoned that if pba were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate. to test this, rabbits with experimental periodontitis were immunized in the hind legs with the histochemically detectable antigen horseradish peroxidase (hrp) or glucose oxidase ( ...	1988	3257940
the potency of immunoglobulin g fragments for inhibition of interference caused by anti-immunoglobulin antibodies in a monoclonal immunoradiometric assay for thyrotropin.	heterophile antibodies in patients' serum may produce false increases in apparent analyte concentrations in "sandwich"-type immunoassays. using three patients with endogenous anti-mouse igg antibodies and a two-site mouse monoclonal assay for thyrotropin, we studied the ability of igg fragments to block this positive interference. mouse whole igg and igg fc fragment blocked the interference virtually completely; igg f(ab')2 and fab fragments did not. rat and horse immunoglobulin fragments gave v ...	1988	3260545
syndromes of vibrio vulnificus infections. clinical and epidemiologic features in florida cases, 1981-1987.	to describe the clinical and epidemiologic features of vibrio vulnificus infections.	1988	3260760
anterior and posterior axial lens displacement and human aqueous outflow facility.	we studied the effect of anterior and posterior axial crystalline lens displacement (and thereby ciliary tone) on the aqueous humor outflow facility in enucleated human eyes. after attaching a reversible footplate plunger to the anterior lens capsule with cyanoacrylate, the lens was placed in one of three positions: "neutral baseline," posterior displacement (2.5 mm), or anterior displacement (2.0 mm). in seven pairs of eyes, the mean "neutral baseline" was not statistically different from the c ...	1988	3262095
anterior and posterior axial lens displacement and human aqueous outflow facility.	we studied the effect of anterior and posterior axial crystalline lens displacement (and thereby ciliary tone) on the aqueous humor outflow facility in enucleated human eyes. after attaching a reversible footplate plunger to the anterior lens capsule with cyanoacrylate, the lens was placed in one of three positions: "neutral baseline," posterior displacement (2.5 mm), or anterior displacement (2.0 mm). in seven pairs of eyes, the mean "neutral baseline" was not statistically different from the c ...	1988	3262095
light and electron microscopic studies of experimentally-induced pathologic changes in the rat periodontal tissue.		1988	3271013
light and electron microscopic studies of experimentally-induced pathologic changes in the rat periodontal tissue.		1988	3271013
vibrio vulnificus: did hippocrates describe a fatal case?	in the fifth century b.c., hippocrates described a fatal illness in a man who lived on an island in the aegean sea. this acute infection was characterized by black bullous skin lesions, rapidly progressive septicemia, and death on the second day. it is hypothesized that this disease was caused by vibrio vulnificus.	1988	3293163
vibrio vulnificus--a new monster of the deep?		1988	3293491
protective immunity evoked by oral administration of attenuated aroa salmonella typhimurium expressing cloned streptococcal m protein.	attenuated strains of salmonella have been used effectively as vaccines against typhoid fever. we have investigated the use of such strains to deliver cloned antiphagocytic virulence determinants of unrelated bacteria. the aroa strain of s. typhimurium sl3261 was transformed with a low-copy plasmid vector pmk207, which contains the cloned gene spm5 encoding streptococcal m protein, the major virulence factor of these organisms. the transformed sl3261 expressed type 5 m protein in the cytoplasmic ...	1988	3294331
ability of vibrio vulnificus to obtain iron from hemoglobin-haptoglobin complexes.	it has been suggested that the normal serum protein, haptoglobin (hp), serves a bacteriostatic role by binding free hemoglobin (hm), thus making heme iron unavailable for bacterial growth. previous studies showed that, unlike escherichia coli, vibrio vulnificus was able to overcome this hp-blocking effect. we report here a study on the iron-withholding property of the three major human hp phenotypes, hp 1, 2, and 2-1. results of experiments with human serum showed that v. vulnificus c7184 was ab ...	1988	3335405
alterations in aortic endothelial cell morphology and cytoskeletal protein synthesis during cyclic tensional deformation.	previous studies have shown that bovine aortic endothelial cells (ecs) in culture respond to repetitive tensional deformation with an increase in deoxyribonucleic acid synthesis and cell proliferation. this study was designed to determine whether cyclic tensional deformation of ecs in vitro induces different morphologic or protein synthetic responses. ecs from passages 6 through 9 were seeded in 35 mm2 well silicone rubber plates at 2 x 10(5) cells/well and allowed to attach for 24 hours. the ex ...	1988	3336119
light- and electron-microscopic analysis of the kidney in newborn rats exposed to gentamicin in utero.	kidneys of newborn rats, whose mothers were given 75 mg/kg of gentamicin daily from the tenth day of gestation to its term, were examined by light and electron microscopy, as were kidneys of control rats whose mothers were given saline during the same period. in the gentamicin-treated group, the numbers of differentiated glomeruli and of s-shaped bodies counted in a median cross-section of the kidney were reduced, compared with those in the control group, but the glomerular volume was the same. ...	1988	3337212
iron metabolism in the erythrophagocytosing kupffer cell.	like the peritoneal macrophage, the isolated kupffer cell is capable of processing and releasing iron acquired by phagocytosis of immunosensitized homologous red blood cells. when erythrophagocytosis is restrained to levels which do not affect cell viability, or less than 1.5 red cells/macrophage (phagocytic index of 150%), over 40% of iron acquired from red cells is released within 24 hr. more active erythrophagocytosis results in greater release of iron but progressive deterioration in cell vi ...	1988	3338718
expression of heme oxygenase gene in rat and human liver.	developmental changes of microsomal heme oxygenase were studied. in human fetal liver, the enzyme activity was 8 times higher than that detected in the adult liver. on the other hand, adult livers contained 4 times more cytochrome p450 than fetal livers. elevated heme oxygenase activity in fetal liver was not due to modulators present in the microsomes. similar to human, rat fetal liver also contained high enzyme activity which appeared to be regulated at the transcriptional level. hybridization ...	1988	3342043
hydrogen transfer by nadph-dependent reductases in elongation of very-long-chain saturated and polyunsaturated fatty-acyl-coa in swine cerebral microsomes.	the hydrogen transfer from nadph was studied in the elongation of arachidoyl-coa (20:0-coa) and arachidonoyl-coa (20:4-coa) in swine cerebral microsomes. previously, we showed that four deuterium atoms (2h) were transferred stereospecifically from (4s)-[4-2h1]nadph in the elongation of 20:0-coa to 24:0 (yoshida, s., takeshita, m. and kawaguchi, a. (1984) biochem. biophys. res. commun. 124, 322-328), and that three deuterium atoms were transferred from 2h2o in the elongation of 20:4-coa to 22:4. ...	1988	3342246
dna binding by 1-nitropyrene and 1,6-dinitropyrene in vitro and in vivo: effects of nitroreductase induction.	1-nitropyrene, the predominant nitropolycyclic aromatic hydrocarbon found in diesel exhaust, is a mutagen and tumorigen. 1,6-dinitropyrene is present in diesel exhaust in much smaller quantities than 1-nitropyrene, but is much more mutagenic and carcinogenic. in an attempt to understand this difference in biological potencies, we have compared the extent of dna binding by these two nitropyrenes in vivo. we have also determined the effect of 1-nitropyrene pretreatment upon the induction of nitror ...	1988	3345577
electron microscope radioautographic evidence of in vivo androgen-binding protein internalization in the rat epididymis principal cells.	the androgen-binding protein (abp) has been purified from rat testes with a yield of 14% using four steps of hplc and was subsequently iodinated to a specific activity of 0.1 mci/mg protein. using a micromanipulator, [125i] iodo-abp-dihydrotestosterone was injected intraluminally into the proximal caput of the rat epididymis. epididymides were sampled from 3 to 120 min after the injection of the tracer and processed for transmission electron microscopy autoradiography. our results showed the acc ...	1988	3345715
early diagnosis of overwhelming vibrio vulnificus infections.		1988	3347871
regional blood flows by the microsphere method: reproducibility in portal hypertensive rats and influence of a portal vein catheter.	to determine the reproducibility of splanchnic blood flow measurements by the microsphere method in rats with portal hypertension and the effects of laparotomy with portal vein cannulation, eight groups of 10 rats were studied. cardiac output and regional blood flows were measured twice, 10 min apart, in pentobarbital anesthetized or awake, sham-operated or portal vein-ligated rats, with or without portal cannulation. variability between the two successive measurements was not affected by portal ...	1988	3353395
purification and characterization of a family of high molecular weight surface-array proteins from campylobacter fetus.	a variety of gram-negative and gram-positive bacteria possess crystalline surface layers, although little is known of their function. we previously have shown that the high molecular weight surface-array proteins of campylobacter fetus are important in both the pathogenicity and antigenicity of this organism. for biochemical and immunological characterization, we purified high molecular weight (100,000, 127,000, 149,000) surface-array proteins from three c. fetus strains using sequential gel fil ...	1988	3360785
evaluation of prostaglandins as mediators of tubuloglomerular feedback.	orthograde and retrograde microperfusion experiments were conducted in sprague-dawley rats to evaluate the participation of vasoconstrictive eicosanoids as mediators of tubuloglomerular feedback (tgf) signals. retrograde perfusion with 160 microm arachidonic acid (aa) added to a hypotonic solution enhanced the stop-flow pressure (sfp) feedback responses compared with those obtained with the control hypotonic solution (delta sfp, 1.6 +/- 0.4 vs. 10.1 +/- 0.7 mmhg with aa). blockade of thromboxane ...	1988	3364575
high-frequency jet ventilation in a neonatal foal.	high-frequency jet ventilation was performed on a premature foal for respiratory difficulty attributable to in utero-acquired pneumonia. the procedure involves delivery of compressed gas through a small-bore cannula at frequencies up to 400 cycles/min. ventilation settings of drive pressure, frequency, and fio2 were varied to optimize pao2 and paco2 values. the foal was ventilated with this equipment for 14 hours. evidence of a favorable response to this method of ventilation was observed in the ...	1988	3366679
neuronal activity recorded extracellularly from in situ canine mediastinal ganglia.	spontaneous activity of 226 neurons was recorded from in situ mediastinal ganglia in 10 dogs. forty-two percent of these were active during specific phases of the cardiac cycle, primarily during systole. cardiovascular-related activity occurred when systolic pressure was between approximately 70 and 185 mmhg (1 mmhg = 133.3 pa) whether the pressure was altered by positive inotropic pharmacological agents or cross clamping of the aorta. twenty percent of the identified neurons displayed respirato ...	1988	3370543
occurrence of vibrionaceae in natural and cultivated oyster populations in the pacific northwest.	studies were done to assess the role of oysters from the pacific northwest as a potential source of vibrionaceae. oysters collected from natural and cultivated populations on the british columbia coast were opened using sterile instruments. the gills and oyster meat were each removed, and the meat was cut in half. the gills and the cut surface of the oyster meat were each cultured by inoculating them directly to the surface of agar plating media for the isolation of vibrionaceae. overall, 120 oy ...	1988	3370928
bacteremia in a veteran's administration extended care facility.		1988	3380833
progesterone regulation of secretory component (sc): uterine sc response in organ culture following in vivo hormone treatment.	sex steroid hormones are known to have profound effects on mucosal immunity. in the present study we evaluated the effects of progesterone on the uterine immune system by determining the changes in the levels of secretory component (sc) released from uterine tissues in culture following in vivo administration of progesterone to estradiol-stimulated ovariectomized rats. sc is a transport protein which moves iga into external secretions such as intestinal and uterine secretions. sc release was det ...	1988	3386246
australian fraud inquiry under way.		1988	3386761
fatal septicaemia caused by vibrio vulnificus.		1988	3399921
vibrio vulnificus septicemia.	we report a case of vibrio vulnificus infection in a middle-aged alcoholic man with laennec's cirrhosis. the patient had recently received a puncture wound from the shell of a shrimp while fishing in the gulf of mexico. he presented with acrally distributed urticarial plaques, purpura, and bullae, as well as signs and symptoms of septic shock. vibrio vulnificus was isolated from the blood, and histologic examination of the skin biopsy specimen demonstrated a devitalized, inflammatory, cell-poor ...	1988	3401105
spinal cord penetration of intrathecally administered cytarabine and methotrexate: a quantitative autoradiographic study.	carcinomatous meningitis is an increasingly common complication of systemic cancer. treatment usually involves the use of intrathecal methotrexate or cytarabine, which have been reported to be toxic to the spinal cord. this study was performed to determine the penetration and distribution of intrathecally administered chemotherapy within the spinal cord. tritiated methotrexate and cytarabine were administered intraventricularly and by lumbar puncture to new zealand white rabbits. the extent and ...	1988	3418727
common genetic pools between human populations.	a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ...	1988	3422217
a mutant human proinsulin is secreted from islets of langerhans in increased amounts via an unregulated pathway.	a coding mutation in the human insulin gene (his-b10----asp) is associated with familial hyperproinsulinemia. to model this syndrome, we have produced transgenic mice that express high levels of the mutant prohormone in their islets of langerhans. strain 24-6 mice, containing about 100 copies of the mutant gene, were normoglycemic but had marked increases of serum human proinsulin immunoreactive components. biosynthetic studies on isolated islets revealed that approximately 65% of the proinsulin ...	1988	3057496
a mutant human proinsulin is secreted from islets of langerhans in increased amounts via an unregulated pathway.	a coding mutation in the human insulin gene (his-b10----asp) is associated with familial hyperproinsulinemia. to model this syndrome, we have produced transgenic mice that express high levels of the mutant prohormone in their islets of langerhans. strain 24-6 mice, containing about 100 copies of the mutant gene, were normoglycemic but had marked increases of serum human proinsulin immunoreactive components. biosynthetic studies on isolated islets revealed that approximately 65% of the proinsulin ...	1988	3057496
probable detection of kil peptide derived from colicin e1 plasmid in the envelope fraction of escherichia coli hb101 carrying peap31.	escherichia coli carrying plasmid peap31 produces extracellularly alkalophilic bacillus penicillinase encoded on the plasmid. the extracellular production has been suggested to be caused by activation of dormant colicin e1 kil gene. two peptides that could be respectively precursor and mature products of colicin e1 kil gene were detected on an sds/polyacrylamide gel. one of the peptides (mr 4800), which was probably a precursor peptide, was detected in the inner-membrane fraction from the organi ...	1988	3058121
probable detection of kil peptide derived from colicin e1 plasmid in the envelope fraction of escherichia coli hb101 carrying peap31.	escherichia coli carrying plasmid peap31 produces extracellularly alkalophilic bacillus penicillinase encoded on the plasmid. the extracellular production has been suggested to be caused by activation of dormant colicin e1 kil gene. two peptides that could be respectively precursor and mature products of colicin e1 kil gene were detected on an sds/polyacrylamide gel. one of the peptides (mr 4800), which was probably a precursor peptide, was detected in the inner-membrane fraction from the organi ...	1988	3058121
high-molecular-weight neutral proteinase of rat ascites hepatoma cells and their collagenolytic activity.	a high-molecular-weight proteinase in rat ascites hepatoma ah109a cells was purified to apparent homogeneity by conventional chromatographic techniques. the purified proteinase degraded over the broad ph range, 7.0-9.0, not only denatured hemoglobin but also type i and iv collagen in the absence of calcium. its activity was maximum at ph 8.0, heat-labile and affected by thiol reagents. serine proteinase inhibitors such as diisopropyl fluorophosphate, soybean trypsin inhibitor, leupeptin, and ant ...	1988	3058624
vitamin e deficiency and neurologic disease.	during the recent resurgence of interest in the clinical uses of vitamin e, one of the major foci of attention has been the neurologic role of vitamin e in humans. studies in patients with secondary vitamin e deficiency, caused by fat malabsorption disorders and total parenteral nutrition lacking an adequate supply of vitamin e, have elucidated a clinical disorder and histologic lesions of the nervous system and muscle that closely resemble those of experimental vitamin-e-deficient animal models ...	1988	3060170
what schedule-induced polydipsia can tell us about alcoholism.	an animal model of chronic and excessive voluntary (unforced) alcohol ingestion is presented in which, by drinking, animals produce repeated, substantial elevations in blood ethanol concentration and develop physical dependence. the overindulgence is elective in that ethanol is chosen in preference to certain other fluid-ingestive alternatives. beside the usual demonstrations of acutely compromised motor performance, tolerance development, cross-tolerance, etc., the model demonstrates that the c ...	1988	3067598
what schedule-induced polydipsia can tell us about alcoholism.	an animal model of chronic and excessive voluntary (unforced) alcohol ingestion is presented in which, by drinking, animals produce repeated, substantial elevations in blood ethanol concentration and develop physical dependence. the overindulgence is elective in that ethanol is chosen in preference to certain other fluid-ingestive alternatives. beside the usual demonstrations of acutely compromised motor performance, tolerance development, cross-tolerance, etc., the model demonstrates that the c ...	1988	3067598
a case of vibrio vulnificus infection.	vibrio vulnificus, a recently described strain of the halophilic vibrio species, was isolated from the blood of a 73-year-old man, who developed rapidly progressive wound infection and fatal septicemia. twenty-one patients with vibrio vulnificus infection have been reported in the japanese literature. vibrio vulnificus most frequently causes primary septicemia and necrotising cellulitis after the eating of raw fish or shellfish or after exposure to seawater. the infection is characterized by its ...	1988	3068394
role of the protease in the permeability enhancement by vibrio vulnificus.	the protease produced by vibrio vulnificus enhances vascular permeability through histamine release from mast cells and activation of the plasma kallikrein-kinin system which generates bradykinin when injected into the dorsal skin. v. vulnificus living cells also enhanced vascular permeability within a few hours after the injection into the dorsal skin. the permeability-enhancing activity of living cells was greatly reduced by addition of soybean trypsin inhibitor, a specific inhibitor for plasm ...	1988	3068493
parasitic diarrhea.	this article reviews the biology, epidemiology, and clinical management of amebiasis, giardiasis, and cryptosporidiosis as well as the less common intestinal protozoa, dientamoeba fragilis, isospora belli, balantidium coli, and blastocystis hominis.	1988	3074123
parasitic diarrhea.	this article reviews the biology, epidemiology, and clinical management of amebiasis, giardiasis, and cryptosporidiosis as well as the less common intestinal protozoa, dientamoeba fragilis, isospora belli, balantidium coli, and blastocystis hominis.	1988	3074123
purification and partial characterization of serum monocytotropic factor, a platelet-derived cyclooxygenase-inducing polypeptide.	it has previously been shown that a heat- and acid-stable component of human and animal sera was capable of stimulating prostanoid biosynthesis in human blood monocytes, very probably by a mechanism involving cyclooxygenase induction. many physico-chemical characteristics of this factor are similar to those of identified platelet factors. here we show that human platelets are a rich source of this factor (serum monocytotropic factor) and that results from experiments using arachidonic acid or th ...	1988	3124880
decreased locomotor activity produced by repeated, but not single, administration of murine-recombinant interferon-gamma in mice.	a single treatment with murine-recombinant interferon-gamma (murifn-gamma; 30 micrograms/mouse), whether evaluated immediately after, or four hrs after, intraperitoneal injection, does not alter open field activity levels. on the other hand, repeated murifn-gamma administration (30 micrograms/mouse/day for 5 days) results in decreased spontaneous locomotor activity and an increase in body weight.	1988	3126371
adult epiglottitis due to vibrio vulnificus.		1988	3130933
hiv seroprevalence in migrant and seasonal farmworkers--north carolina, 1987.		1988	3136308
is vitellogenin an ancestor of apolipoprotein b-100 of human low-density lipoprotein and human lipoprotein lipase?	vitellogenin, an ancient animal protein, is the major yolk protein of eggs, where it is used as a food source during embryogenesis. here it is shown that vitellogenins, including those from the invertebrates caenorhabditis elegans and drosophila melanogaster, contain domains that are homologous with parts of apolipoprotein b-100 (apob-100) of human low-density lipoprotein and human lipoprotein lipase. as vitellogenins are likely to have been used by invertebrates during embryogenesis well before ...	1988	3145737
poor lifestyle modification among intravenous drug users assisted at a sexually transmitted disease clinic for hiv infection diagnosis, madrid, 1987.		1988	3146273
isolation of the early phase of chylomicron formation in intestinal epithelial cells of rats.	lipid is first observed electron microscopically in the rough endoplasmic reticulum of intestinal epithelial cells during active lipid absorption. we have been able to isolate this subcellular fraction by using discontinuous sucrose gradients of 0.25/0.86/1.11 m sucrose. a preliminary low speed centrifugation of mucosal homogenate removed the heavier subcellular organelles. the resulting supernatant was centrifuged at 5.25 x 10(6) x g.min. the pellet from this centrifugation was placed on top of ...	1988	3147719
biosynthesis and processing of platelet gpiib-iiia in human megakaryocytes.	platelet membrane glycoprotein iib-iiia forms a calcium-dependent heterodimer and constitutes the fibrinogen receptor on stimulated platelets. gpiib is a two-chain protein containing disulfide-linked alpha and beta subunits. gpiiia is a single chain protein. these proteins are synthesized in the bone marrow by megakaryocytes, but the study of their synthesis has been hampered by the difficulty in obtaining enriched population of megakaryocytes in large numbers. to examine the biosynthesis and pr ...	1987	3108266
stimulation by hyphopichia burtonii and bacillus amyloliquefaciens of aflatoxin production by aspergillus flavus in irradiated maize and rice grains.	aspergillus flavus was grown on maize and rice extract agars and on irradiated viable cracked maize and rice grains, either in pure culture or in dual culture with wild strains of either hyphopichia burtonii or bacillus amyloliquefaciens. aflatoxin production by a. flavus and its growth and interactions with the other microorganisms were studied at three water activities (aw) (0.98, 0.95, and 0.90) and two temperatures (25 and 16 degrees c). both h. burtonii and b. amyloliquefaciens markedly sti ...	1987	3111368
comparison of glycosidase levels in bovine seminal plasma.	seven glycosidases (beta-n-acetylglucosaminidase, alpha-fucosidase, beta-galactosidase, acid alpha-glucosidase, beta-glucuronidase, acid and neutral alpha-mannosidase) were analysed in seminal plasma from the first and second successive ejaculates in normal ayrshire bulls. in comparison to our previous data the results indicate that beta-n-acetylglucosaminidase, beta-galactosidase and beta-glucuronidase are derived mainly from epididymal secretions, while alpha-fucosidase and particularly neutra ...	1987	3112030
inhibitory effect of capsular antigen of vibrio vulnificus on bactericidal activity of human serum.	opaque (op) and translucent (tr) colonial variants were isolated from vibrio vulnificus strains. op-type variants were more resistant than the isogenic tr-type variants, but the survival rate of the op-type variants varied with the strains. antisera were prepared by immunizing rabbit with whole cells of op and tr variants of some strains, in which the difference of the sensitivity between op and tr cells was remarkable. then agglutination tests with their living and heat-killed cells were carrie ...	1987	3116370
purification of an octamer sequence (atgcaaat)-binding protein from human b cells.	the highly conserved octamer sequence atgcaaat or its inverse complement found in all human and murine immunoglobulin gene promoters has been demonstrated to be necessary in the lymphoid-specific transcription by deletion analysis. trans-acting factors that interact with the octamer motif are thought to be involved in this tissue-specific expression. using a gel mobility shift assay, we have identified both lymphoid-specific and ubiquitous nuclear factors that interact with a human gamma 1 heavy ...	1987	3122182
[primary leiomyosarcoma of the pulmonary artery. apropos of a case].	a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ...	1987	3122693
covalent coupling of 4-thiouridine in the initiator methionine trna to specific lysine residues in escherichia coli methionyl-trna synthetase.	a new method has been developed to couple a lysine-reactive cross-linker to the 4-thiouridine residue at position 8 in the primary structure of the escherichia coli initiator methionine trna (trnafmet). incubation of the affinity-labeling trnafmet derivative with e. coli methionyl-trna synthetase (metrs) yielded a covalent complex of the protein and nucleic acid and resulted in loss of amino acid acceptor activity of the enzyme. a stoichiometric relationship (1:1) was observed between the amount ...	1987	3122828
[study on prostaglandin e involved in tooth movement--immunohistochemical observation].		1987	3475379
comparative biodistributions and rates of catabolism of radiolabelled anti-cea monoclonal antibody and control immunoglobulin in nude mice with human tumour xenografts showing specific antibody localization.	the rate of catabolism of a radiolabelled monoclonal anti-cea antibody has been compared with that of control igg1 in control nude mice and in mice with cea producing xenografts and antigen negative xenografts. the rate of catabolism of antibody, but not of igg, was increased 3-4 fold in mice with xenografts localizing the antibody, but not in mice with antigen negative tumours. there was no evidence of immune complex formation and/or clearance of antibody from the serum of xenografted mice and ...	1987	3478194
variation in the lymphocyte transformation assay. slope analysis of cell dose-response curves.	blood samples from a single sheep were cultured at a number of different cell concentrations and stimulated with three lymphocyte mitogens, phytohaemagglutinin, concanavalin a and pokeweed mitogen. the response to stimulation, measured as log thymidine uptake (log cpm) was found to be a linear function of log cell concentration (log cell dose). samples taken on different dates, varied significantly in the gradients of the log cpm: log cell dose plots. these alterations of gradient constituted th ...	1987	3571985
aids and non-human primates.		1987	3576926
radiosensitivity and sublethal damage repair in human umbilical cord vein endothelial cells.	the assessment of the comparative radiosensitivity of different parenchymal cells is potentially of great importance in clinical radiotherapy. previous studies from various laboratories using vessel regeneration techniques have demonstrated that endothelial cells are quite radioresistant, having a do in excess of 250 rads. the present studies were undertaken to investigate the radiosensitivity of human endothelial cells obtained from umbilical cord veins (hec) using "colony formation" as an endp ...	1987	3583859
testicular defect: the primary abnormality in gonadal dysfunction of uremia.	gonadal dysfunction is a common problem in end-stage renal disease. we decided to test the ability of the male gonads to respond to exogenous stimuli. the patients receiving chronic hemodialysis showed abnormally low levels of total testosterone and free testosterone in the presence of normal testosterone-binding globulin. furthermore, the exogenous administration of human chorionic gonadotropin failed to elicit a normal response from the male gonads of hemodialyzed patients. although basal seru ...	1987	3589761
noninvasive doppler assessment of human postprandial renal blood flow and cardiac output.	we compared the effects of protein, carbohydrate, or water ingestion on human renal blood flow (rbf) and cardiac output (co). we validated and applied a noninvasive ultrasonic doppler method to measure blood velocities and lumen diameters in the right renal artery and the ascending aorta of five healthy adults. from these measurements, we calculated the average rbf and co over five cardiac cycles. normalizing to body surface area, renal blood flow index to one kidney (ri) and cardiac index (ci) ...	1987	3591956
storage conditions and stability of thyrotropin and thyroid hormones on filter paper.	we evaluated the effects of temperature and humidity on thyroid hormones (t4, t3) and thyrotropin (tsh) measured in blood spots dried on filter paper. rias for t4 and t3 blood spots were optimized to measure these analytes over the neonatal and euthyroid adult reference intervals. sensitivities of the t3 and t4 assays were 0.5 and 10 nmol/l, respectively. a blood-spot immunoradiometric assay for tsh involving magnetizable particles was developed with a sensitivity of 6 milli-int. units/l. contro ...	1987	3594835
storage conditions and stability of thyrotropin and thyroid hormones on filter paper.	we evaluated the effects of temperature and humidity on thyroid hormones (t4, t3) and thyrotropin (tsh) measured in blood spots dried on filter paper. rias for t4 and t3 blood spots were optimized to measure these analytes over the neonatal and euthyroid adult reference intervals. sensitivities of the t3 and t4 assays were 0.5 and 10 nmol/l, respectively. a blood-spot immunoradiometric assay for tsh involving magnetizable particles was developed with a sensitivity of 6 milli-int. units/l. contro ...	1987	3594835
fibre types in extraocular muscles: a new myosin isoform in the fast fibres.	we report on the existence of a myosin heavy chain (mhc) isoform with unique structural properties in extraocular (eo) muscles. differences in mhc composition are apparent using a polyclonal antibody prepared against myosin isolated from bovine eo muscle myosin. in enzyme immunoassays and western blotting experiments, this anti-eo myosin antibody reacted specifically with the heavy chains of eo muscle myosin and not with the heavy chains of other myosins. the distribution of this new mhc isoform ...	1987	3597767
fibre types in extraocular muscles: a new myosin isoform in the fast fibres.	we report on the existence of a myosin heavy chain (mhc) isoform with unique structural properties in extraocular (eo) muscles. differences in mhc composition are apparent using a polyclonal antibody prepared against myosin isolated from bovine eo muscle myosin. in enzyme immunoassays and western blotting experiments, this anti-eo myosin antibody reacted specifically with the heavy chains of eo muscle myosin and not with the heavy chains of other myosins. the distribution of this new mhc isoform ...	1987	3597767
thin collagenous septa in cardiac muscle.	light and electron microscopy were used to study the structure and distribution of thin collagenous septa (sheets) in dog and rabbit cardiac muscle to determine whether they, like thick collagenous septa, could affect electrical impulse propagation. generally, thin septa (0.2-0.5 micron) ensheathed myocytes or groups of myocytes for short distances and thicker septa partially or completely ensheathed groups of myocytes for long distances (up to several mm); together, thin, and thick septa divide ...	1987	3605660
virulent strains of vibrio vulnificus isolated from estuaries of the united states west coast.	vibrio vulnificus was isolated from united states west coast estuaries at a low frequency (5.9%) from 529 samples of water, shellfish, and sediment. four strains tested with iron-treated mice had 50% lethal dose values ranging from 7.6 to 360 cfu, compared with a 50% lethal dose of 4.9 cfu for a clinical isolate that caused the death of a septicemic patient. the presence of this pathogen may be a hazard to users of marine beaches and consumers of raw shellfish on the west coast, especially to pe ...	1987	3606112
plasmid-mediated trimethoprim-resistance in staphylococcus aureus. characterization of the first gram-positive plasmid dihydrofolate reductase (type s1).	the trimethoprim-resistance gene located on plasmid psk1, originally identified in a multi-resistant staphylococcus aureus from australia, encodes the production of a dihydrofolate reductase (type s1), which confers a high degree of resistance to its host and is quite unlike any plasmid-encoded dihydrofolate reductase hitherto described. it has a low mr (19,700) and has a higher specific activity than the constitutive gram-negative plasmid dihydrofolate reductases. the type s1 enzyme is heat-sta ...	1987	3606580
[analysis of lethal toxin produced by vibrio vulnificus].		1987	3610014
differing resistance to polymorphonuclear cells of halophilic- and hypotonic-types of vibrio vulnificus.	bacterial growth and lethality of 4 strains of vibrio vulnificus infection of mice were enhanced by gamma-irradiation but not by treatment with carrageenan. therefore, protection against v. vulnificus, at least in the early phases, probably depends mainly on polymorphonuclear cells (pmn), since carrageenan depletes macrophages but not pmn. pmn dependent protection against infection differs for 2 types of v. vulnificus strains. halophilic- and hypotonic-type were distinguished from the correspond ...	1987	3612754
intrinsic circuitry involving the local axon collaterals of corticothalamic projection cells in mouse smi cortex.	the objective of this study was to identify the components involved in a local synaptic circuit in the mouse cerebral cortex. the local axon collaterals of corticothalamic (ct) projection cells in the posteromedial barrel subfield area of primary somatosensory cortex were labeled by the retrograde transport of horseradish peroxidase injected into the ipsilateral thalamus. thalamocortical (tc) axon terminals in the same region of cortex were labeled by lesion induced degeneration. ct axon termina ...	1987	3624546
prenatal diagnosis of minute 5p- deletion: a cytogenetic problem in detection.	an unbalanced translocation, 46,xx,der(5)t(5;11) (p15;q25)mat was ascertained by prenatal diagnosis. the mother previously had a liveborn child with cri du chat syndrome. the subtlety of the chromosome rearrangement in this case illustrates the need for the most detailed cytogenetic investigations in cri du chat cases when deletion or translocation are not immediately obvious.	1987	3627600
activation of the plasma kallikrein-kinin system by vibrio vulnificus protease.	vibrio vulnificus protease enhanced hypodermic vascular permeability when injected into the dorsal skin of a guinea pig. enhancement of permeability was observed within 2 min, and the permeability-enhancing reaction terminated at about 10 min postinjection. the permeability-enhancing reaction was greatly augmented by simultaneous injection of a kininase ii inhibitor, whereas the reaction was inhibited by soybean trypsin inhibitor, a well-known inhibitor of plasma kallikrein. furthermore, in vitr ...	1987	3649335
nonradioactive, photobiotin-labelled dna probes for the routine diagnosis of barley yellow dwarf virus.	photobiotin was used to prepare biotinylated, nonradioactive nucleic acid probes for the detection of the rna of barley yellow dwarf virus (bydv) in plant extracts. a 1.7-kb cdna of the pav isolate of bydv in the plasmid puc8 vector was biotinylated and then used intact or as sonicated double-stranded dna fragments. simple methods were developed for the preparation of partially purified nucleic acid extracts of cereals and their spotting, after formaldehyde treatment, onto nitrocellulose membran ...	1987	3654900