Publications

normal response to thyrotrophin releasing hormone (trh) in familial thyroxine-binding globulin deficiency.	the response in serum thyrotrophin (tsh) to thyrotrophin releasing hormone (trh) has been studied in 5 euthyroid patients with familial thyroxine-binding globulin (tbg) deficiency. total serum thyroxine (t4), serum triiodothyronine (t3) and free t4 index and free t3 index were significantly and equally decreased, but in spite of these findings the serum tsh and response to trh was normal. the trh test seems to be a better indicator of the euthyroid state in familial tbg deficiency than the measu ...	1975	52266
thyroxine utilization rate in sucking pigs.	in 10 suckling pigs age 28--34 days, thyroxine utilization rate (tur) was determined by means of the isotope dilution procedure. for this purpose biological half-life of t4 was estimated during 96 h of blood sampling and serum t4 concentration was measured directly by a competitive protein-binding technique. high rate of the peripheral hormonal-iodine metabolism (from 10.3 +/- 0.6 to 21.2 +/- 1.2 mug t4/kg/day) was found in the one-month old pigs. tur value calculated for these pigs corresponds ...	1975	52283
[excessive peripheral conversion of thyroxine (t4) to triiodothyronine (t3) in the pathogenesis of t3-hyperthyroidism (author's transl)].	in a 41-year-old woman and a 3-year-old girl, both of them with t3-thyrotoxicosis, serum levels of total and free t4 and t3 were measured serially during anti-thyroid drug treatment. attempts to substitute thyroxine during the antithyroid treatment had to be interrupted because the patients became hyperthyroid again with excessive increases in total and free serum t3, even when concentrations of total and free t4 were brought to subnormal levels. the increased conversion of administered thyroxin ...	1975	52434
determinants of the hierarchy of humoral immune responsiveness during ontogeny.	a model system of ontogeny was utilized to investigate the development of humoral immunity in both akr and balb/c mice. lethally irradiated adult mice were reconstituted with syngeneic fetal or neonatal liver. these mice were immunized at various times after reconstitution with a series of eight antigens: the bacteriophages f2, phix-174, and t4; the hapten carrier complexes 2,4 dinitrophenyl-bovine serum albumin and fluorescein-bovine serum albumin; and the small proteins: hen egg lysozyme, sper ...	1975	52670
[correlation studies of some thyroid function tests (author's transl)].	the results of a thyroid test based on ion exchange and column chromatographic separation and of two radiochemical t4 and three t3 tests were carried out with commercially available reagent packs ready for use, and were compared with the pbi values found in the same serum sample in each case. this also tested the analytical precision of each method. a good correlation was found between the pbi and the t4 values, while the accuracy of the t3 tests was unsatisfactory.	1975	52835
[proceedings: pituitary thyroid axis in subacute thyroiditis (author's transl)].	we studied 10 patients with subacute thyroiditis. all had an increase in free thyroxine index, pbi, t4 and pbi/t4 ratio, an absence of thyroidal radioiodine uptake and no increase in plasma tsh after trh injection. exogenous tsh increased the uptake in 6/9 patients. the three patients who did not respond to tsh had higher pbi/t4 ratio than others. after treatment for one month there was either a normalization of laboratory tests either a subnormal thyroid function associated with an absence of r ...	1975	53025
a temporal component of the auditory evoked response.	we studied the 75-225 msec portion of the auditory evoked response (aer) in 32 normal adults at vertex (cz) and temporal (t3 and t4) placements referred to a balanced, noncephalic reference electrode using a monaural 1 msec click stimulus delivered every 4.7 sec at 60 db above threshold. the tape-recorded eeg was filtered at 1-25 c/sec, and 128 individual responses were summed, sampling every 0.5 msec for 250 msec post-stimulation. the cz aers showed the classic vertex response, a negative peak, ...	1975	53139
triiodothyronine and thyroid-stimulating hormone in protein-calorie malnutrition in infants.	protein-calorie malnutrition (p.c.m.) in a group of 43 senegalese children aged eighteen to thirty months was characterised by a sharp fall in serum-triiodothyronine (t3) concentration to 25-3% of the mean value in healthy age-matched controls. this decrease in t3 was significantly (p less than 0-001) more pronounced in kwashiorkor of recent onset than in long-term p.c.m., a finding which suggests that impaired thyroxine (t4) monodeiodination in the liver was responsible for the fall in serum-t3 ...	1975	53331
value of routine serum-triiodothyronine estimation in diagnosis of thyrotoxicosis.	thyroid-stimulating hormone (t.s.h.), triiodothyronine (t3), and thyroxine (t4) concentrations were estimated on 432 blood specimens from patients with a provisional diagnosis of thyrotoxicosis. 59 patients had a raised serum t3 concentration with t.s.h. and t4 levels in the normal range. further information was obtained in 41 of these patients, and t3 toxicosis was diagnosed in 17 cases, thus providing a projected total of 24 patients with t3 toxicosis. in addition, there were 56 patients, with ...	1975	53496
[influence of hypo- and hyperthyroidism on the turnover rate of noradrenaline, dopamine and serotonin in various rat cerebral structures].	the effect of chronic treatment with tyroxine (t4) or propylthiouracile (ptu) on the turnover of norepinephrine (ne), dopamine (da) and 5-hydroxytryptamine (5-ht) has been studied in various areas of the rat brain (brain stem, hypothalamus, striatum and "rest of the brain"). the turnover of ne and da was determined by the decay in endogenous levels after inhibition of tyrosine hydroxylase by alpha-methylparatyrosine and the turnover of 5-ht was evaluated by the initial accumulation of endogenous ...	1975	54127
thyroidal triiodothyronine and thyroxine in graves' disease: correlation with presurgical treatment, thyroid status, and iodine content.	to evaluate the potential contribution of thyroidal secretion to the relative excess of triiodothyronine (t3) production in hyperthyroidism and to investigate the effects of treatment, iodine (127i), t3 and thyroxine (t4) were measured in digests of thyroid tissue obtained at surgery from 13 patients with graves' disease. in 11 normal human thyroid glands, 127i content was 630 +/- 60 (all values mean +/- se in mug/ wet weight) t4, 254 +/- 39 and t3 21 +/-3. the t4i was 26 +/- 3% of the total iod ...	1975	54364
serum triiodothyronine and reverse triiodothyronine concentrations after surgical operation.	serum-triiodothyronine (t3) concentrations fell rapidly after surgery in six out of seven initially euthyroid patients. simultaneous increases in reverse triiodothyronine (rt3) concentrations suggested that the peripheral monodeiodination of thyroxine (t4) proceeds by an alternative pathway in the postoperative period.	1975	54799
[diagnostic staged program in thyroid diseases].	after some physiological principal remarks the modern possibilities of the in-vitro-diagnostics specific for the thyroid gland are briefly explained. the essential factors of disturbance which are necessary for the avoidance of false interpretations are mentioned. from the sequence of the description (bej, t4-test, t3-test, ft4-index, ria-t3 and ria-tsh) results the diagnostic step programme proposed by us, by means of which the majority of the functional disturbances of the thyroid gland existi ...	1975	55003
characterization of a bacteriophage t4 mutant lacking dna-dependent atpase.	a dna-dependent atpase has previously been purified from bacteriophage t4-infected escherichia coli. a mutant phage strain lacking this enzyme has been isolated and characterized. although the mutant strain produced no detectable dna-dependent atpase, growth properties were not affected. burst sizes were similar for the mutant phage and t4d in pola1, recb, recc, uvra, uvrb, uvrc, and various dna-negative e. coli. uv sensitivity and genetic recombination were normal in a variety of e. coli hosts. ...	1975	123009
high-resolution scanning electron microscopy of bacteriophages 3c and t4.	an account is presented of the design and operation of a new scanning electron microscopic, and its first application to the study of biological samples. bacteriophages were chosen because much of their ultrastructure is beyond the resolution of the conventional scanning electron microscope. the new instrument permits examination of bulk samples with a resolution that exceeds, by at least a factor of 2.5, the resolution obtained in the best secondary electron scanning electron microscopes using ...	1975	125922
[iodoamino acid composition of rat thyroglobulin of varying total iodine concentration and fractions isolated by isopycnic ultracentrifugation].	iodoaminoacid content (iodothyronines, t3 and t4, and iodotyrosines, mit and dit) has been determined in enzymatic hydrolysates of thyroglobulin tg 19s of different iodine content (0.3-0.9%) isolated from equilibrium labeled rats. preparative equilibrium centrifugation in rbcl density gradients of pure thyroglobulin was used to obtain protein fractions of largely different iodine content (0.2-1.2% i). thin layer chromatography of total hydrolysates demonstrated that the distribution of iodoamino ...	1975	126748
immunochemical studies on tyrosinase induction in neurospora.	an immunoassay for tyrosinase, using the modified bacteriophage technique, was developed: tyrosinase of neurospora was conjugated to bacteriophage t4 using glutaraldehyde as a cross-linking agent. the conjugated phage that survived the coupling process could be inactivated by antiserum raised in rabbits against pure tyrosinase, but not by normal serum. this inactivation was specifically inhibited by pure neurospora tyrosinase, and the degree of inhibition was proportional to the concentration of ...	1975	128453
triiodothyronine binding to isolated liver cell nuclei.	nuclei of euthyroid rat liver have been prepared from homogenates by sedimentation through 2.3m sucrose with or without a 0.25% triton wash. triiodothyronine is accumulated by these nuclei during incubation in vitro in solutions containing 0.32m sucrose, 1mm mgcl2 and 0.02m tris-cl buffer at ph 7.4 or 7.85. specific t3 binding sites occupied at 10-1,000 pm t3 are saturated by excess unlabeled t3 (0.15 mum). specific t3 binding at 20 c is maximal at 203 hr nad is proportional to amount of nuclei. ...	1975	163184
temporal appearance of bacteriophage t4-modified valyl trna synthetase in escherichia coli.	bacteriophage t4-induced modification of escherichia coli vlayl-trna synthetase (ec 6.1.1.9) requires: synthesis of a phage-gene specified tau factor, addition of the factor to host valyl-trna synthetase to produce a urea-stable enzyme, and interaction of the modified enzyme with trna to produce a more rapidly sedimenting valyl-trna synthetase activity on sucrose density gradients. this report demonstrates that the coincident, chloramphenicol-sensitive appearance of urea-stable and rapidly sedim ...	1975	163351
mutations altering genetic recombination and repair of dna in bacteriophage t4.		1975	163533
effect of salts and polyamines on t4 polynucleotide kinase.	the activity of t4 polynucleotide kinase (ec 2.7.1.78) was found to be greatly stimulated by salts, such as nacl and kcl, and polyamines such as spermine and spermidine. up to a sixfold increase in initial rates was observed with a variety of different single-stranded dnas and mono- and oligonucleotides. the optimal concentrations of salts were 0.125 m, corresponding to a total ionic strength of mu equals 0.19. for polyamines the optimal concentrations were found to be at approximately 2 mm. wit ...	1975	164211
effects of thyroid and ovaries on prolactin binding activity in rat liver.	125i-radiolabeled ovine prolactin (oprl) binding activity was measured in microsomal membranes of liver tissue from intact, ovariectomized, ovariectomized-thyroidectomized, and ovariectomized-thyroidectomized rats injected with thyroxine (t4) or estradiol benzoate (eb). thyroidectomy and ovariectomy each reduced prl binding activity in liver tissue significantly. the combination of ovariectomy and thyroidectomy decreased prl binding activity more than thyroidectomy or ovariectomy alone. doses of ...	1975	164346
the ultraviolet endonuclease of bacteriophage t4. further characterization.	the t4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated dna on the 5' side of thymine dimers. the present studies demonstrate that the purified endonuclease creates 3'-oh and 5'-p termini at the sites of nicking. photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded escherichia coli and m-13 dna.	1975	164454
reversible hypothyroidism in growth hormone-deficient children treated with human growth hormone.	six children with human growth hormone (hgh) deficiency became hypothyroid during the course of their therapy with hgh. this was accompanied by a decreasing growth rate, clinical symptoms of hypothyroidism and decreased serum t4 concentrations. three of the 6 patients returned to the euthyroid state, both clinically and biochemically, with cessation of hgh therapy, and reinstitution of hgh precipitated hypothyroidism again in 2 of the three. the patients who remained hypothyroid have evidence of ...	1975	165211
effects of thyroid hormone administration on skeletal muscle mitochondria.	the effects of thyroid hormone administration on the levels of a number of mitochondrial markers were measured in skeletal muscle and liver of normal rats. injection of 18 mug of l-thyroxine (t4) per 100 g body wt every 4th day for 3 wk had no effect on the concentrations of cytochrome c, on citrate synthase activity, or on respiratory capacity of skeletal muscle. injection of 200 mug of l-triiodothyronine (t3) daily for 5 days, or feeding 23 mg t4 and 7 mg t3/kg of diet for 2 wk, resulted in th ...	1975	165730
effect of oral thyrotrophin-releasing hormone on serum thyroxine in growth hormone deficient and normal children.	oral administration of synthetic trh in a dose of 80 mg/1-73 m-2 at 0 and 12 h to normal and constitutionally small children caused a significant increase of total serum thyroxine (t4) within 6-24 h. the mean maximal t4 increment was +3-7 plus or minus 1-1 and +3-8 plus or minus 1-2 mug/dl (mean plus or minus 1 sd) respectively in the two groups. of seventeen euthyroid gh deficient children, fifteen showed a normal and two patients a slightly subnormal response. of fifteen hypothyroid gh deficie ...	1975	165912
role of gene 59 of bacteriophage t4 in repair of uv-irradiated and alkylated dna in vivo.	nonsense mutants in gene 59 (amc5, amhl628) were used to study the role of this gene in the repair of uv-damaged and alkylated dna of bacteriophage t4 in vivo. the higher sensitivity to uv irradiation and alkylation of gene 59 mutants after exposure to these agents was established by a comparison of the survival fractions with wild type. zonal centrifugal analysis of both parental and nascent mutant intracellular dna molecules after uv irradiation showed that immediately after exposure the size ...	1975	166207
pathways of dna repair in t4 phage. i. methyl methanesulfonate sensitive mutant.		1975	166482
effect of iodized oil on iodine content, thyroglobulin maturation and on biochemical constituents of endemic goitre in brazil.	the biochemical and morphological changes in thyroid glands were studied in 5 patients with endemic goitre in the region before and one year after the administration of iodized oil. each patient received a tracer dose of 125-i 3-5 weeks before the biopsy and another tracer of 131-i 12 h before surgery. it was observed that: 1. iodine deficient goitrous glands had more protein per gram of tissue and that this was corrected by iodination. 2. the goitrous glands exhibited a higher relative proporti ...	1975	166539
effect of infection with gonococci on myeloperoxidase activity of leucocytes.	lyosomal myeloperoxidase activity in human phagocytic leucocytes was stimulated by incubation with virulent (t1) and avirulent (t4) forms of neisseria gonorrhoeae. the amount of activity, assayed by bacterial iodination (125-iodine) after 30 min. exposure to the pagocytes in the absence of serum, was about fifty times greater in cells infected with t4 strains. in the presence of heated human serum, or its igg component, myeloperoxidase activity increased, but t1-stimulated activity was significa ...	1975	166724
nucleotide sequence studies of normal and genetically altered glycine transfer ribonucleic acids from escherichia coli.	the total nucleotide sequence of trnagga/g -gly2 from escherichia coli is pg-c-g-g-g-c-a-u-c-g-u-a-u-a-a-u-g-g-c-u-a-u-u-a-c-c-u-c-a-g-c-c-u-n-c-c-a-a-g-c-u-g-a-u-g-a-u-g-c-g-g-g-t-psi-c-g-a-u-u-c-c-c-g-c-u-g-c-c-c-g-c-u-c-c-aoh, where t- at position 53 is ribothymidylic acid, and psi- at position 54 is pseudouridylic acid; n- at position 36 is an unidentified derivative of uridylic acid, and is present in modified form in a portion of trnagga/g -gly 2 molecules isolated from e. coli cells. the ...	1975	167016
effect of dna-negative and maturation-defective conditions on accumulation of functional messengers for t4 bacteriophage-specific dihydrofolate reductase and deoxynucleoside monophosphate kinase.	messengers for t4 phage-specific deoxynucleoside monophosphate kinase overaccumulated in nonpermissive hosts infected with amber-defective viruses that displayed either the dna-negative or maturation-defective phenotype. under both conditions, however, transcription of functional messengers for dihydrofolate reductase followed essentially normal kinetics.	1975	167197
denaturation of polyoma dna by phage t4 gene 32 protein.		1975	167284
e. coli membrane lipid alteration affecting t4 capsid morphogenesis.		1975	167290
synthesis of functional bacteriophage t4-delayed early mrna in the absence of protein synthesis.	when escherichia coli b207 is grown either aerobically or under limited aerobic conditions, pretreated with chloramphenicol to block protein synthesis, and then infected with bacteriophage t4, the phage rna which accumulates, termed "immediately early" (ie), contains the transcripts of a limited number of prereplicative genes. among the transcripts which accumulate is the mrna which serves as a template for deoxycytidylate hydroxymethylase (hmase) synthesis. among the prereplicative gene transcr ...	1975	168406
the effect of iodized oil on the tsh response to trh in endemic goiter patients.	the tsh and t3 response to synthetic trh was evaluated in 4 groups of patients: normal controls and goitrous subjects from the urban area of sao paulo (urinary iodine excretion: 172.2 +/- 48.3 mug i/g creatinine) and nongoitrous and goitrous subjects from the endemic areas of sao bento (urinary iodine excretion: 53.8 +/- 17.1 mug i/g). plasma t4 and t3 were within our normal range in all groups of patients. the mean plasma tsh was significantly higher (5.2 +/- 3.3 muu/ml) in goitrous subjects li ...	1975	169288
in vitro repair of uv-or x-irradiated bacteriophage t4 dna by extract from blue-green alga anacystis nidulans.	the cell-free extract from blue-green alga anacystis nidulans contains enzymatic activities which repair in vitro transforming dna of bacteriophage t4 damaged by uv light or x-rays. the repair effect of the extract was observed with double-stranded irradiated dna but not with denatured irradiated dna. the level of restoration of the transforming activity depends on the protein concentration in the reaction mixture and on the dose of irradiation. a fraction of dna lesions induced by x-rays is rep ...	1975	169464
influence of thyroid hormone on glycogen metabolism in rat liver.	the livers removed from thyroidectomized and l-t4 supplemented rats were rapidly frozen by freon-12 chilled with liquid nitrogen, and concentrations of metabolites which affect glycogen synthetase and phosphorylase were determined. serum and liver glycose levels were not changed in any thyroid functioning. but liver g6p and atp were increased by thyroidectomy and decreased by l-t4 supplement, while camp was increased by the hormone supplement. the "enzyme activity" ratio of glycogen synthetase a ...	1975	170073
location of the t4 gene 32 protein binding site on polyoma virus dna.		1975	170135
letter: the isolation and properties of the specific binding sites for escherichia coli rna polymerase on t4 and t7 bacteriophage dnas.		1975	170407
effect of chloramphenicol and starvation for an essential amino acid on the synthesis and decay of t4 bacteriophage-specific messengers transcribed from early and quasi-late promoters.		1975	170861
end group labelling of rna and double stranded dna by phosphate exchange catalyzed by bacteriophage t4 induced polynucleotide kinase.		1975	170940
3,3',5'-triiodothyronine (reverse t3) and 3,3',5-triiodothyronine (t3) in fetal and adult sheep: studies of metabolic clearance rates, production rates, serum binding, and thyroidal content relative to thyroxine.	to examine the mechanism(s) responsible for high serum concentration of 3,3',5'-triiodothyronine (reverse t3, rt3) and low serum concentration of 3,3',5-triiodothyronine (t3) in the fetus, we studied metabolic clearance rates (mcr) and production rates (pr) of rt3, t3, and thyroxine (t4) in adult nonpregnant sheep and sheep fetuses in utero. the mean fetal mcr-rt3 was significantly lower than that in adult sheep, and the mean fetal pr-rt3 significantly higher. the mean fetal mcr-t3 was higher th ...	1975	171141
inhibition of the response of mouse thyroid to tryrotropin induced by chronic triiodothyronine treatment.	administration of 1 mu bovine tsh iv to mice resulted, within 1 hour, in the increase of the serum t4 level from 32 +/- 1.4 ng/ml to 53 +/- 2.6 ng/ml (mean +/- se, n = 24). treatment with 1 mug triiodothyronine (t3) per day, for 10 days, abolished the responsiveness of the thyroid to tsh, as measured by thyroxine (t4) release. thyroidal response to tsh was measured also in vitro. the basal hormonal release was 4.66 +/- 0.55 ng t4 and 0.98 +/- 0.15 ng t3 per thyroid per 3 h (n = 30). in the prese ...	1975	171149
human lymphocyte binding and deiodination of thyroid hormones in relation to thyroid function.	the human lymphocyte has been investigated regarding its function as a thyroid hormone target cell. binding and deiodination of the thyroid hormones were determined after simultaneous incubation of 131i-labelled l-thyroxine (131i-t4) and 125i-labelled l-triiodothyronine (125i-t3) with lymphocytes from healthy subjects, from hyperthyroid and primary hypothyroid patients before and after treatment. the mean percentages of binding, 8.0 +/- 0.5 (mean +/- sem) for 131i-t4, and 9.7 +/- 0.4 for 125i-t3 ...	1975	171899
competitive inhibition of transformation in group h streptococcus strain challis by heterologous deoxyribonucleic acid.	glucosylated deoxyribonucleic acid (dna) from phages t4 and t6 competes poorly with homologous dna causing only a slight decrease of transformation in group h streptococcus strain challis. other types of heterologous dnas (micrococcus luteus, clostridium perfringens, escherichia coli, calf thymus and non-glucosylated phage t6 dna), in contrast to glucosylated t4 and t6 dnas, compete with transforming dna to the normal, high extent. these results indicate that as in transformation of bacillus sub ...	1975	172490
restoration of ultraviolet-induced unscheduled dna synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage t4 endonuclease v and hvj (sendai virus).	ultraviolet (uv)-induced unscheduled dna synthesis of xeroderma pigmentosum cells, belonging to complementation groups a, b, c, d, and e, was restored to the normal level by concomitant treatment of the cells with t4 endonuclease v and uv-inactivated hvj (sendai virus). the present results suggest that (1) t4 endonuclease molecules were inserted effectively into the cells by the interaction of hvj with the cell membranes, (2) the enzyme was functional on human chromosomal dna which had been dama ...	1975	172893
adp-ribosylation of dna-dependent rna polymerase of escherichia coli by an nad+: protein adp-ribosyltransferase from bacteriophage t4.	a protein from bacteriophage t4 responsible for the alteration of host dna-dependent rna polymerase and absent in t4 alt- phage was purified from t4 phage and enriched from t4-infected cells. it is injected during infection together with the known internal proteins. it has a molecular weight of about 70000 and catalyses the release of nicotinamide and the transfer of the adp-ribosyl moiety from nad+ to arginyl residues of various proteins including itself. rna polymerase from escherichia coli ac ...	1975	173540
kinetics and effect of salts and polyamines on t4 polynucleotide ligase.	the kinetics of t4 polynucleotide ligase has been investigated at ph 8,20 degrees c and using the double-stranded dna substrate (da)n - [(dt)10]n/10. double-reciprocal plots of initial rates vs substrate concentrations as well as product inhibition studies have indicated that the enzyme reacts according to a ping-pong mechanism. the overall mechanism was found to be non-processive. the true km for the dna substrate was 0.6 mum and that of atp 100 mum. several attempts were made to reverse the t4 ...	1975	173544
human papillomavirus dna: physical map.	human papillomavirus (hpv) dna form i (supercoiled) was prepared from plantar warts. hpv dna was cleaved with restriction enzymes obtained from the following sources: escherichia coli (ecori), hemophilus influenzae strain rd (both unfractionated hind and aeparated hindii and hindiii enzymes) and hemophilus parainfluenzae (hpai). the cleavage products were analyzed by polyacrylamide gradient slab gel electrophoresis and electron microscopy. hpv dna was cleaved into two fragments by ecori (87% and ...	1975	174077
the production of undegraded cytosine-containing dna by bacteriophage t4 in the absence of dctpase and endonucleases ii and iv, and its effects on t4-directed protein synthesis.		1975	175166
cyclic nucleotide phosphodiesterase from bone: characterization of the enzyme and studies of inhibition by thyroid hormones.	studies were carried out to characterize the cyclic nucleotide phosphodiesterase from rat calvaria. 25-40% of the total enzyme activity was membrane-bound. ph, magnesium, and temperature requirements conformed closely to those established for phosphodiesterase from other tissues. kinetic evidence was found for dual enzyme activities with different substrate affinities for both the particulate and soluble enzyme. apparent kms for the soluble enzyme (3.5 times 10-6 and 2.5 times 10-5m) approximate ...	1975	234382
kinetics and specificity of t4 polynucleotide kinase.	the kinetics of t4 polynucleotide kinase has been investigated at ph 8.0 and 37 degrees. double reciprocal plots of initial rates vs. substrate concentrations as well as product inhibition studies have indicated that the enzyme reacts according to the ordered sequential mechanism shown in eq 2 in the text for phosphorylation of a dna molecule. based on this mechanism the rate equation for the overall reaction was deduced and the various kinetic constants estimated. hill plots indicated little or ...	1975	235284
triiodothyronine binding to liver nuclear solubilized proteins in vitro.	nuclear proteins extracted from purified nuclei with 0.4m kcl at ph 7.4 or 8.5 are able to bind l-triiodothyronine (t3) giving rise to nuclear thyroid hormone binding protein-t3 (ntbp-t3) complexes. binding is maximum in 3 h at 20 c. it is thermolabile even at 36 c, inhibited by p-hydroxymercuribenzoate and markedly enhanced by dithiothreitol. optimum ph is between 7.8 and 8.5. divalent cations are not necessary. the ntbp-t3 complex exhibits similar anodal electrophoretic migration in polyacryla ...	1975	235420
thioredoxin reductase-mediated hydrogen transfer from escherichia coli thioredoxin-(sh)2 to phage t4 thioredoxin-s2.	thioredoxin from escherichia coli b and phage t4-infected e. coli b are small hydrogen carrier proteins which in their reduced forms are specific hydrogen donors to e. coli and t4-induced ribonucleotide reductase, respectively. the oxidation-reduction active group of both thioredoxins consists of a single cystine residue which is reduced to sulfhydryl form by nadph in the presence of e. coli thioredoxin reductase. reduction of t4 thioredoxin-s2 to thioredoxin-(sh)2 led to a 3-fold increase in th ...	1975	235528
phage t4 lysozyme. physical properties and reversible unfolding.	phage t4 lysozyme has been used extensively in studies of the genetic code. however, little work has been done on the characterization of the purified enzyme. therefore, we determined the spectral properties of native t4 lysozyme and used these properties to follow the unfolding transition. the ultraviolet absorption spectrum and solvent perturbation difference spectrum indicate that the aromatic amino acids are extensively exposed to solvent. the cd and ord spectra are characteristic of a high ...	1975	236026
accelerated host metabolism of l-thyroxine during acute infection: role of the leukocyte and peripheral leukocytosis.	metabolism of thyroid hormones is accelerated during acute infection in man and in experimental animals. the pathogenetic mechanisms mediating this phenomenon are uncertain, but activated leukocytes of the infected host have been implicated as potentially important sites of hormone degradation. the present studies were conducted in an attempt to assess the in vivo contribution of leukocytes and peripheral leukocytosis to the enhancement of l-thyroxine (t4) clearance seen during infection, and to ...	1975	236322
the lipopolysaccharides of neisseria gonorrhoeae colony types 1 and 4.	the lipopolysaccharides (lps) of strains of neisseria gonorrhoeae grown in type 1 (t1) and 4 (t4) colony forms have been isolated. lps from t4 colony type cells on mild hydrolysis gave a lipid a and a core oligosaccharide composed of 2-amino-2-deoxy-d-glucose, d-glucose, d-galactose, l-glycero-d-manno-heptose and 3-deoxy-d-manno-octuosonic acid that appeared to be common to all the strains examined. lps from t1 colony type cells on mild hydrolysis gave a lipid a and high molecular weight o polys ...	1975	237620
gene 21 protein-dependent proteolysis in vitro of purified gene 22 product of bacteriophage t4.		1975	238040
excision of bromodeoxyuridine from t4-dna by an antimutator polymerase of t4 phage.	with gene-43 (dna polymerase)-ts-mutants of t4 phage, l98 (mutator) and cb121 (antimutator), and the t4 wild type, double labelling of dna was carried out with (h3)-bromodeoxyuridine (budr) and (c14)-thymidine (tdr). experiments on (c14) tdr for dna synthesis measurement in the presence of budr offered evidence of the ability of the cb121 mutant to excise budr from the dna. this effect took place only at increased temperature. as distinct from dna synthesis of the host, all t4 phages used prefer ...	1975	239571
polyacrylamide gel electrophoresis of intact bacteriophage t4d particles.	a method for the electrophoresis of intact bacteriophage t4d particles through polyacrylamide gels has been developed. it was found that phage particles will migrate through dilute polyacrylamide gels (less than 2.1%) in the presence of a low concentration of mgcl2. as few as 5 x 10(9) phage particles can be seen directly as a light-scattering band during the course of electrophoresis. the band can also be detected by scanning gels at 260 to 265 nm or by eluting viable phage particles from gel s ...	1975	240037
studies of the self-association of bacteriophage t4 gene 32 protein by equilibrium sedimentation.		1975	241852
bacteriophage t4 whiskers: a rudimentary environment-sensing device.	the 400 a filaments or "whiskers," which extend outward from the collar region of the phage, control retraction and extension of the tail fibers in response to certain environmental conditions. the tail fibers of normal phage retract in the absence of a required adsorption cofactor, at low ph, at low ionic strength, at low temperature, and at high concentrations of polyethylene glycol. the tail fibers of mutant whiskerless (wac) phage still retract under the first two conditions, but not the las ...	1975	242007
in vitro synthesis of enzymes of the tryptophan operon of escherichia coli. evidence for positive control of transcription.	a protein fraction, called at (= anti termination) factor, has been isolated from extracts of e. coli and partially purified. the at factor stimulates the synthesis in vitro of anthranilate synthetase, an enzyme encoded by two genes of the tryptophan (trp) operon, but has no effect on the synthesis of t7 rna polymerase and other t7- and t4 coded proteins. the at factor stimulates the synthesis of trp mrna; it has no effect on the translation of trp mrna. we conclude that in vitro transcription o ...	1975	16094972
e. coli k12 inf: a mutant deficient in prophage lambda induction and cell filamentation.	the bacterial mutant inf-3 (lambda) is not inducible and does not form filaments following thymine starvation. lysogenic induction is neither produced by ultraviolet light (uv) nor promoted by tif-1. this phenotype is due to a mutation infa3 located between 60 and 73 min on the e. coli k12 map. the inf mutant is resistant to x-ray and uv irradiation, in contrast to all other known non-inducible bacterial mutants. it is rec+ and able to perform host cell reactivation as well as uv-reactivation of ...	1975	16094997
unaltered stability of newly synthesized rna in strains of escherichia coli missing a ribonuclease specific for double-stranded rna.	pairs of very closely related escherichia coli strains were prepared, one having the wild-type allele for ribonuclease iii, an enzyme which specifically degrades double-stranded rna, and the other having a mutant rnase iii allele. growth and phage plating efficiency were compared in these strains. the rnase iii+ strains grow better than the rnase iii- strains and plate t7 and lambda phage better, but t4 plates with the same efficiency on both strains. on the other hand, the half lives of newly s ...	1975	16094999
structure of herpes simplex virus dna: topography of the molecule. i. absence of circularly permuted sequences.	only linear structures were produced by re-annealing denatured herpes simplex type 1 virus (hsv)-dna molecules, while t4-dna molecules (known to be circularly permuted and therefore used as controls in the parallel tests) formed circles. these results suggested that hsv-dna is a nonpermuted collection of sequences. unfortunately, most of the linear structures observed after re-annealing were not full-length duplexes, thus making this test not quite satisfactory. therefore another permutation tes ...	1975	18621351
a gene of bacteriophage t4 controlling the modification of host valyl-trna synthetase.	two hydroxylamine-induced mutants of bacteriophage t4 defective in modification of host valyl-trna synthetase have been isolated by assay of crude extracts for the activity that is characteristic of the wild-type virus. the mutations define a single gene that is situated between the ri and e genes on the t4 genetic map. this new gene is designated vs for valyl-trna synthetase. one of the mutations may be of the missense type since it results in the production of a valyl-trna synthetase activity ...	1975	18621354
new late gene, dar, involved in dna replication of bacteriophage t4 i. isolation, characterization, and genetic location.	suppressors of gene 59-defective mutants were isolated by screening spontaneous, temperature-sensitive (ts) revertants of the amber mutant, amc5, in gene 59. six ts revertants were isolated. no gene 59-defective ts recombinant was obtained by crossing each ts revertant with the wild type, t4d. however, suppressors of gene 59-defective mutants were obtained from two of these ts revertants. these suppressor mutants are referred to as dar (dna arrested restoration). dar mutants specifically restore ...	1975	16789147
[determination of thyroid hormone-induced metabolic conditions using the t4 and t3 tests with reference to radioiodine therapy].		1974	4134611
lysogeny and lysosensitivity in shigella dysenteriae group of bacteria. 3. serological specificity of receptors of t2 and t4 phages in shigella dysenteriae 7, strain d7.		1974	4137048
specificity of antibodies to the modified dna of phages spo1 and t4.		1974	4207745
[plasma tsh and circulating thyroid hormone (t3 and t4) levels in patients with goiter and endemic cretinism].		1974	4208746
transient lack of thyrotropin (tsh) response to thyrotropin-releasing hormone (trh) in treated hyperthyroid patients with normal or low serum thyroxine (t4) and triiodothyronine (t3).		1974	4208806
classification of hypothyroidism in evaluating patients after radioiodine therapy by serum cholesterol, t3-uptake. total t4, ft4-index, total t3, basal tsh and trh-test.		1974	4208979
relative rates of transcapillary movement of free thyroxine, protein-bound thyroxine, thyroxine-binding proteins, and albumin.	the rate of appearance of labeled thyroxine (t4) and albumin in lymph from various areas after simultaneous i.v. injection of the labeled substances in conscious ambulatory sheep has been used to estimate the relative rates of transcapillary movement of stable t4 and albumin. labeled t4 appeared in hepatic lymph at the same rate as albumin. in intestinal and leg lymph, labeled t4 appeared eight and four times as rapidly as albumin indicating that t4 crosses capillaries in these areas independent ...	1974	4209664
the absence of a pyrimidine dimer repair mechanism in mammalian mitochondria.	we have investigated whether mammalian cells can repair pyrimidine dimers in their mitochondrial dna which have been induced by ultraviolet light. the assay system is based upon the ability of the phage t4 uv endonuclease to nick covalently closed circular mitochondrial dna that contain pyrimidine dimers. our results show that dimers are not removed from the mitochondrial dna of mouse l cells or human kb and hela cells. there is also no evidence for photoreactivation of mitochondrial dna. analys ...	1974	4212385
[proceedings: use of the peroral trh-t4 test in endocrinologic diagnosis].		1974	4212779
hyperresponse to thyrotropin-releasing hormone accompanying small decreases in serum thyroid hormone concentrations.	to determine whether pituitary thyrotropin (tsh) responsiveness to thyrotropin-releasing hormone (trh) is enhanced by small decreases in serum thyroxine (t4) and triiodothyronine (t3), 12 euthyroid volunteers were given 190 mg iodide po daily for 10 days to inhibit t4 and t3 release from the thyroid. basal serum t4, t3, and tsh concentrations and the serum t4 and tsh responses to 400 mug trh i.v. were assessed before and at the end of iodide administration. iodide induced small but highly signif ...	1974	4214837
isolation by affinity chromatography of a precursor head protein (p23) of bacteriophage t4.		1974	4214934
bacterial mutation affecting t4 phage dna synthesis and tail production.		1974	4215034
phase transition of gonococci in mammalian cell cultures.	neisseria gonorrhoeae was cultivated in mammalian cell cultures in an effort to determine if this environment will elicit a t4 --> t1 transition. of four avirulent (t4) isolates tested, only one, h4, yielded t1 colonies. this change was consistently obtained in hela, wi-38, and mk2 cells, even when the multiplicity of the gonococcal infection was less than 1 per culture. growth of the gonococci took place primarily on the surface of the cells, as demonstrated by light and electron microscopy, bu ...	1974	4215765
[t4 bacteriophage irradiation in a 1 million volt microscopemprelimnary results].		1974	4216431
[proceedings: effect of t4 binding protein on thyroid hormone secretion].		1974	4217707
[endocrine opthalmopathy-- new physiopathological viewpoints through the determination of t3, t4 and tsh following trh stimulation].		1974	4217981
[serum level of thyroxine (t4) and triiodothyronine (t3) in patients with congenital goiter].		1974	4220004
control of oxygen consumption in liver slices from normal and t4-treated rats.		1974	4277420
inhibition of t4 growth by an rna polymerase mutation of escherichia coli: physiological and genetic analysis of the effects during phage development.		1974	4278501
initiation characteristics for the synthesis of five t4 phage-specific messenger rnas in vitro.	the involvement of the nucleoside triphosphates in the initiation of the synthesis of the messenger ribonucleic acid of five t4 specific enzymes has been studied. only one of these, the messenger rna for deoxynucleosidemonophosphate kinase, can be initiated in the presence of one nucleoside triphosphate, namely atp. all of the remaining four require the presence of at least two nucleoside triphosphates during the initiation period. the combination of atp and utp was best for the initiation of me ...	1974	4360943
genetics and physiology of bacteriophage t4 3'-phosphatase: evidence for involvement of the enzyme in t4 dna metabolism.	mutants of bacteriophage t4d which fail to induce the deoxyribonucleotide-specific t4 3'-phosphatase have been isolated. these mutants (t4pset) grow as well as wild-type t4 in most strains of escherichia coli, but not in the t4-sensitive "hospital strain," ct196, or in a derivative strain, ctr5x. both the formation of infectious centers and the final yield of phage are reduced by 98% when ctr5x is infected by t4pset mutants. the growth defects are accompanied by a 50% reduction in the rate of t4 ...	1974	4362515
characterization of new regulatory mutants of bacteriophage t4.	plating techniques which eliminate t4 plaque formation on escherichia coli by folate analogue inhibition of dihydrofolate (fh(2)) reductase (ec 1.5.1.3) allowed the isolation of folate analogue-resistant (far) mutants of t4. one class of far mutants overproduces the phage-induced fh(2) reductase. deoxycytidylate deaminase (ec 3.5.4.12), thymidine kinase (ec 2.7.1.21), and deoxycytidine triphosphatase (ec 3.6.1.12) are also overproduced by 20 min after infection at 37 c. the overproduction of fh( ...	1974	4362869
location of the t4 gene 32 protein-binding site on polyoma virus dna.		1974	4364559
[treatment of primary lipoproteinemia type iia and iib with highly purified dextro-thyroxine (d-t4). controlled study in ambulant conditions].		1974	4365703
the induction of deoxythymidine kinase by bacteriophage t4.		1974	4367304
transcriptional regulation of t4 bacteriophage-specific enzymes synthesized in vitro.	in contrast to dihydrofolate reductase and four other phage-specific enzymes, the initiation of deoxynucleotide kinase is essentially prevented if rifampin is added to a culture of escherichia coli b cells within 1.5 min after infection with t4. deoxynucleotide kinase thus belongs to a group of so-called delayed-early enzymes that is not initiated from an immediate-early promoter site. we prepared crude extracts from infected cells in a manner designed to maintain the integrity of the complexes ...	1974	4367905
chemical structure of a modification of the escherichia coli ribonucleic acid polymerase alpha polypeptides induced by bacteriophage t4 infection.		1974	4371081
deletion analysis of two nonessential regions of the t4 genome.		1974	4371653
thyroid hormone action: in vitro characterization of solubilized nuclear receptors from rat liver and cultured gh1 cells.	we previously reported that putative nuclear receptors for thyroid hormone can be demonstrated by incubation of hormone either with intact gh(1) cells, a rat pituitary tumor cell line, or with isolated gh(1) cell nuclei and rat liver nuclei in vitro. we characterized further the kinetics of triiodothyronine (t3) and thyroxine (t4) binding and the biochemical properties of the nuclear receptor after extraction to a soluble form with 0.4 m kcl. in vitro binding of [(125)i]t3 and [(125)i]t4 with gh ...	1974	4372251
[mutagenic effect of nalidixic acid on bacteriophage t4 (author's transl)].		1974	4372526
studies on ribonucleic acid ligase. characterization of an adenosine triphosphate-inorganic pyrophosphate exchange reaction and demonstration of an enzyme-adenylate complex with t4 bacteriophage-induced enzyme.		1974	4373468