Publications

thyroid metabolism in the recessive sex-linked dwarf female chicken. 5. effects of exogenous thyroid hormones on amino acid uptake by eggs.	the incorporation of l-methionine-3h(g) and l-lysine-4,5'3h(n) into egg albumen by dwarf and non-dwarf white leghorn hens was studied together with the influence of exogenous triiodothyronine (t3) and tetraiodothyronine (t4) on those incorporation patterns. the results were influenced by the amino acid studied and by the strain of bird. the maximum uptake of methionine was a day 1 for the normal and at day 4 for the dwarf which suggested that the dwarf was storing methionine in the tissue first ...	1975	1178609
[deiodase activity in hypothyroid rats].	in rats hypothyroidized with methylthiouracil (mtu), methimazol (mmi), or radiothyroidectomy, the extent of deiodination for l-diiodotyrosine (l-dit) and l-thyroxine (l-t4) was investigated in homogenate supernatants of liver and kidney. deiodination in liver and kidney for dit is twice as high as for t4, but the kidney allows only 25% of the liver deiodination activity both for dit and t4. in the livers of all hypothyroid animals, iodide splitting both from dit and t4 is highly significantly re ...	1975	1179918
the thyrotoxicosis of hydatidiform mole.	in 14 women with hydatidiform mole, 9 were hyperthyroid. serum thyroxine (t4) levels varied between 18 and 34 mug/100 ml, and serum triiodothyronine (t3) levels between 300 and 800 ng/100 ml in the hyperthyroid patients. bioassayable thyroid-stimulating hormone (molar tsh) was found in high concentrations in the serum of 13 patients in whom preoperative serum was available. there was a close correlation between the serum levels of human chorionic gonadotrophin, molar tsh, and t3. intravenous sod ...	1975	1180425
comparison of the predicted and observed secondary structure of t4 phage lysozyme.	predictions of the secondary structure of t4 phage lysozyme, made by a number of investigators on the basis of the amino acid sequence, are compared with the structure of the protein determined experimentally by x-ray crystallography. within the amino terminal half of the molecule the locations of helices predicted by a number of methods agree moderately well with the observed structure, however within the carboxyl half of the molecule the overall agreement is poor. for eleven different helix pr ...	1975	1180967
experimental cretinism in lambs: an intrauterine model with thyroid evaluation in surviving lambs.	the experiments in this article were designed to establish an animal model analogous to human athyrotic cretinism without altering maternal thyroid status and subsequently to observe the natural course of the disorder. the effect of ovine intrauterine fetal thyroidectomy during the second trimester was assessed by following maternal thyroid function and amniotic fluid t4 by column [t4(c)], t3 by radioimmunoassay (t3ria), and ovine tsh (otsh). maternal serum t4(c) rose during pregnancy but unexpe ...	1975	1181470
influence of histamine- and 5-hydroxytryptamine-containing thyroid mast cells on thyroid blood flow and permeability in the rat.	the possible significance of thyroid mast cells in the regulation of thyroid blood flow and capillary permeability was investigated in rats whose tsh secretion had been eliminated by exogenous t4. mast cells were identified by their abundance of metachromatic granules, and their content of histamine and 5-hydroxytryptamine (5-ht) was examined by fluorescence histochemistry. thyroid histamine levels were determined by fluorometry. the tissue uptake of 86rb was used as an indicator of blood flow a ...	1975	1183406
effect of acute exposure to cold on the activity of the hypothalamic-pituitary-thyroid system.	the effects of a sudden but sustained exposure to cold (1 to 6c) on serum tsh, thyroxine (t4) and triiodothyronine (t3) (all measured by radioimmunoassay), pituitary tsh concentration, pituitary tsh secretory responsiveness to hypothalamic extract or synthetic thyrotropin-releasing hormone (trh) in vitro as well as in vivo, and the changes of the thyrotropin-releasing activity in three trf-rich hypothalamic areas were determined. in normal animals, serum tsh underwent a series of oscillations, f ...	1975	1183407
changes in plasma thyrotrophin, thyroxine, and triiodothyronine after acute or chronic administration of iodide to iodine-deficient rats.	the effect of chronic oral or acute iv administration of small graded doses of iodide was studied in severely iodine-deficient rats. drinking water supplemented with 0.1, 0.2, or 0.4 mug 127i-/ml was given for up to 42 days. plasma t3, which was initially in the normal range, increased 2--3-fold on days 1 and 2 after initiation of iodide supplementation, then dropped to approximately the intial concentration at day 3. plasma t4, initially undetectable, was calculated to increase approximately 20 ...	1975	1183408
thyroxine-induced hyperthyroidism upon the reproductive performance of female rats.	l-thyroxine (l-t4) was injected into mature rats daily at levels of 0, 3, 6, 12, 24, 48 and 96 mug/100 g body weight through estrous cycling, conception, pregnancy, parturition and 20 days of lactation. mothers which lactated to 20 days were killed and mammary glands measured for dna and rna content. those which were not able to maintain their pups were killed on the day when all pups died. estrous cycling and pregnancy were not markedly effected by exogenous l-t4 at levels 3-96 times the normal ...	1975	1183934
action of bacteriophage t4 ultraviolet endonuclease on duplex dna containing one ultraviolet-irradiated strand.	previous studies have indicated that the ultraviolet endonuclease of bacteriophage t4 acts specifically at pyrimidine dimer sites in ultraviolet-irradiated dna. at such sites the enzyme could conceivably catalyze endonucleolytic incision of the dna either on the dimer-containing strand or on the strand directly opposite to the dimer. in the present work, a direct test of these alternatives was made. substrate molecules containing one irradiated and one unirradiated strand were prepared from diff ...	1975	1184590
the effect of short-term triiodothyronine administration on thyroxine response to exogenous tsh in man.	in order to examine whether thyroid hormone concentration interfere with the thyroid gland responsivity to tsh, paired studies on the effect of short-term t3 treatment on t4 response to exogenous tsh ( 5 i.u. i.m.) were carried out in 10 euthyroid volunteers. during t3 administration ( 120 mcg/day for 4 days starting 48 hr before tsh injection) a significant decrease in t4 concentrations was observed both prior to and after tsh, with an inhibition of the t4 response ranging from 36-77% as calcul ...	1975	1184728
a radioimmunoassay for serum rat thyroglobulin. physiologic and pharmacological studies.	a double antibody radioimmunoassay has been developed to measure thyroglobulin in rat (rtg) serum. the lowest detectable quantity measurable was 5.0 ng/ml. specificity was documented by: (a) fall in serum rtg to undetectable levels after thyroid ablation; (b) the fact that l-thyroxine, d-thyroxine, l-triiodothyronine, d-triiodothyronine, triiodothyroacetic acid, tetraiodothyroacetic acid, triiodothyropropionic acid, moniodotyrosine, diiodotyrosine, and human thyroglobulin (htg) in concentrations ...	1975	1184735
a comparative study of serum total thyroxine estimation on unextracted serum by radioimmunoassay and by competitive protein binding.	a rapid and precise radioimmunoassay (ria) for serum total thyroxine (t4) on as little as 1-10 mul of unextracted serum is described. results in hypothyroidism (overt and borderline), in euthyroid subjects in pregnant and oestrogen-medicated subjects, and in hyperthyroidism (overt and borderline) are compared with the results on the same sera by an established competitive protein binding technique (ames' tetralute) on unextracted serum from a different laboratory. the correlation between the two ...	1975	1184759
structural aberrations in t-even bacteriophage. vii. in vitro analysis of the canavanine-mediated inhibition of proteolytic cleavage.	canavanine arrests a critical function in head morphogenesis and the potential for forming giant t-even phage particles termed lollipops is induced. formation of the particles requires the addition of arginine and the restoration of normal functions. we now report on an investigation into the effects of canavanine on both the t4-induced proteolytic activity and on the substrate proteins. using an in vitro cleavage assay we have shown that the gene 21-dependent proteolytic activity from canavanin ...	1975	1185853
das mutation in bacteriophage t4d does not suppress an amber mutation in t4 gene 59.	mutations termed das were isolated originally (hercules and wiberg, 1971) as partial suppressors of mutants in phage t4 genes 46 and 47. since mutants in genes 46, 47, and 59 exhibit both an early arrest of phage dna synthesis and the loss of this arrest in the presence of chloramphenicol or of mutations of t4 genes 33 and 55, we asked whether a das mutation can also suppress a gene 59 mutant. we find that it cannot--either at the level of phage production or dna synthesis.	1975	1185855
radiotherapy in the treatment of t3-t4 supraglottic tumors.		1975	1186397
effect of dexamethasone on thyroid hormone response to tsh.	the effect of short-term dexamethasone administration (8 mg daily for 3 days) on thyroid hormone response to exogenous tsh (bovine tsh, 5 iu i.m.) was studied in 16 euthyroid volunteers. serum t3 and t4 concentrations were measured by radio-immunoassay prior to and 2, 6, 12, 24, and 49 hr after btsh injection, both under basal conditions and during dexamethasone treatment. in all subjects btsh administration raised both t3 and t4 concentrations significantly. dexamethasone treatment induced a sl ...	1975	1186494
thyroid hormone metabolism in amniotic and allantoic fluids of the sheep.	amniotic and allantoic fluid samples were obtained at the time of uterotomy from healthy pregnant ewes at various time during gestation. in most instances maternal and fetal blood was collected simultaneously. serum thyroxine (t4) and triiodithyronine (t3) and free t4 were measured in most samples. to determine whether conjugastes of t4 were presented in amniotic and allantoic fluids. t4 was measured before and after 24-hr acid hydrolysis. t4 and t3 turnover from amniotic fluid were measured usi ...	1975	1187248
alteration in tissue and serum concentrations of tsh, iodide, t4 and t3 induced by various dietary iodide levels.	isotopic equilibrium and radioimmunoassay methods were used to evaluate the effects of increases in iodide intake on tissue and serum concentrations of thyroid hormones. within the range of iodide levels used total iodine in peripheral tissues and serum increase directly with iodide intake but this change is mainly due to an increase in inorganic iodide. it is concluded that increases in tissue thyroid hormone concentrations occur within a relatively narrow range of iodide intake and maximal con ...	1975	1187686
kinetic measurement of t4 following column chromatography.	a kinetic measurement of the eluates from the column purification of t4 is described. the choice of cation exchange chromatography to reduce interfering x-ray contrast substances, and the influence of protein in the reaction have been evaluated. data for precision, inter and intra assay reproducibility, detection limit and normal values are reported. using sample volumes of 0.5 ml and an analysis time of one minute, the proposed method compares favourably with published methods.	1975	1189526
radiography of laryngeal carcinoma. assessment of value.	the value of various radiographic methods (except laryngography) in laryngeal carcinoma was assessed in a material of 71 patients. radiography proved of no value in t1 and t2 tumours if a satisfactory clinical examination could be performed but gave valuable information in recurrences as well as in t3 and t4 cases.	1975	1189963
repair of heteroduplex dna in bacteriophage t4.		1975	1191173
postreplication repair in human cells: on the presence of gaps opposite dimers and recombination.	human cells prelabeled with [32p]phosphate were exposed to uv and then pulse-labeled with [3h]thymidine. the dna from these cells was subsequently treated with t4 endonuclease v, an enzyme which specifically nicks dna at positions adjacent to pyrimidine dimers. sedimentation in alkaline sucrose gradients revealed that both the dna made before and that made after irradiation contained nuclease-sensitive sites, indicating that a recombinational process between these dnas might be occurring during ...	1975	1191186
effects of amiodarone on thyroid function in patients with ischaemic heart disease.	thyroid function was evaluated clinically and biochemically in 12 patients with ischaemic heart disease receiving 200 mg oral amiodarone three times daily for periods up to 6 weeks. during drug administration, no patient developed clinical or laboratory evidence of hypothyroidism, but serum levels of t3 tended to fall and those of t4 increased but not to levels outside the normal range. amiodarone produced a significant reduction in heart rate with prolongation of the qtc interval of the electro ...	1975	1191447
[carcinoma of the nasopharynx (cavum) : clinical and developmental aspects].	between 1959 and 1974, two hundred and ninety six carcinomas of the nasopharynx were seen at the institut gustave-roussy, in adults. 78% were in man. the most frequently encountered were undifferentiated or poorly differentiated forms, 76% of cases. 38% only of the patients had their birthplace in france. on the first examination 36% of the cases had a t4 tumour, 50% had fixed cervical lymph nodes and 77% had distant metastases. 30% of the cases could not be treated following a protocol attempti ...	1975	1191790
bacteriophage t4 trnas: structure, genetics, and biosynthesis.		1975	1192243
inactivation of bacteriophages with cis-platinum(ii) diamminedichloride.	the ability of the cis-plantinum(ii) diamminedichloride and its hydrolytic products to inactive dna bacteriophages was examined on the models t2, t4, t4bo1, t3, and lambda. the inactivation of all bacteriophages under study increases gradually during the first 40-90 min of the action of neutral cis-pt(ii) and later passes into an exponential phase. the extent of the region of slower inactviation is larger for osmotically sensitive strains t2 and t4. inactivation with the hydrolytic products for ...	1975	1192548
high-affinity binding of tetraiodothyroacetic acid by a prealbumin in normal rabbit serum.	a protein that binds tetraiodothyroacetic acid (tetrac) with high specificity has been detected in normal rabbit serum. scatchard plots revealed the protein to have a principal binding site with both high capacity and high affinity for tetrac (ka 4.8 x 10(10) m-1. binding of tetrac by the protein is partially inhibited by barbital. during polyacrylamide gel electrophoresis at ph 8.0, the tetrac binding protein has a mobility characteristic of a prealbumin. as judged from competitive binding stud ...	1975	1193013
acute effects of thyroxine, triiodothyronine, and iodide on thyrotropin secretion.	rats fed a purina or low-iodine diet (lid) for varying periods were serially sampled before and after a single iv injection of t4, t3, iodide or saline. suboptimal replacement doses of t4 and t3 were given to rats fed lid for 2 months or 1 year (basal tsh, approximately 1000 and 2000 muu/ml, respectively). both 1 mug t4 and 0.25 mug t3/100 g bw dropped plasma tsh to 70% of the initial level at 15 min and to 10--20% at 4 h. by 12 h tsh had begun to rise in 2-month lid rats, followed by a secondar ...	1975	1193014
the role of replication proteins in the regulation of bacteriophage t4 transcription. ii. gene 45 and late transcription uncoupled from replication.		1975	1195366
host mutant (tabd)-induced inhibition of bacteriophage t4 late transcription. i. isolation and phenotypic characterization of the mutants.		1975	1195367
protein cleavage during virus assembly: characterization of cleavage in t4 phage.		1975	1195380
secondary structure change of bacteriophage t4 sheath protein during sheath contraction.		1975	1195404
the effect of iodine deficiency on thyroid function in the infant rat.	we have studied rats born to severely iodine-deficient mothers and subsequently maintained on a low-iodine diet (lid) from birth to 41 days. they were compared with controls born to mothers fed a high-iodine diet (hid). lid babies from birth on had large goiters, high thyroid labeled mit/dit ratios and radioiodine uptakes, high plasma tsh and low plasma t4 in comparison to hid controls. thyroid labeled t3/t4 ratios were low in all babies at birth but were higher in the lid than hid babies from d ...	1975	1196133
energy-dependent activation of the temperature-sensitive dna polymerases induced by bacteriophage t4 gene 43 mutants.		1975	1198924
two suppressor loci for gene 49 mutations of bacteriophage t4. i. genetic properties and dna synthesis.		1975	1198925
two suppressor loci for gene 49 mutations of bacteriophage t4. ii. dna and capsid maturation.		1975	1198926
radiation failures in cancer of the larynx.	overwhelming statistics have been published regarding the success of radiation therapy in the management of laryngeal carcinoma. the fate of those patients whose cancers are not controlled by radiotherapy is often left to speculation. an analysis is made of 61 patients with early and advanced laryngeal cancer treated initially with radiation therapy and subsequently operated upon because of recurrent or persistent tumor. frequently, diagnosis of recurrence is difficult or delayed. preservation o ...	1975	1200570
effect of althesin (ct-1341) on thyroid-adrenal function in man.	the effects of althesin on adrenocortical and thyroid function were investigated by measuring plasma concentrations of cortisol, thyroxine (t4) and free thyroxine index (fti) in 29 surgical patients. althesin did not change the plasma cortisol concentrations from the preanaesthetic control value; however, the concentrations increased significantly after the start of surgery and thereafter. althesin anaesthesia per se decreased slightly, but significantly, plasma t4 concentration, but did not inf ...	1975	1201161
[specific fragmentation of phage t4 dna by ecori endonuclease].		1975	1201711
studies on the specificity of action of bacteriophage t4 lysozyme.	lysozyme from bacteriophage t4 was found to digest a soluble, uncrosslinked peptidoglycan which is secreted by cells of micrococcus luteus when incubated in the presence of penicillin g. analysis of the enzymatic degradation products shows that t4 acts as an endo-acetylmuramidase capable of cleaving glycosidic bonds only at muramic acid residues that are substituted with peptide side-chains. the results indicate that the secreted peptidoglycan may consist of a mixture of chains, approximately ha ...	1975	1201752
bacteriophage t4 baseplate components. iii. location and properties of the bacteriophage structural thymidylate synthetase.	two t4d thymidylate synthetase (td) temperature-sensitive mutants have been isolated and characterized. both mutants produce heat-labile phage particles. this observation supports the view that this viral-induced protein is a phage structural component. further, antiserum to td has been shown to block a specific step in tail plate morphogenesis. the results indicated that the td protein is largely covered by the t4d tail plate gene 11 protein. since the phageinduced dihydrofolate reductase (dfr) ...	1975	1202242
identification of p48 and p54 as components of bacteriophage t4 baseplates.	the involvement of two bacteriophage t4 gene products in the initiation of t4 tail tube and sheath polymerization on mature baseplates has been studied by radioautography of acrylamide gels of various partially completed tail structures. the products of genes 48 and 54 (p48[the nomenclature p48 refers to the protein product of bacteriophage t4 gene 48] and p54), which are known to be required for the synthesis of mature baseplates, have been shown to be structural components of the baseplate. th ...	1975	1202250
studies of endocrine and affective functions in complex flight manoeuvres.	endocrine and metabolic changes, as well as affective functions, were studied in eight healthy volunteers anticipating and executing a prearranged sequence of aerobatic flight. control measurements were made at complete physical and mental rest. the following were determined: anxiety and hostility levels, blood glucose, cholesterol, triglyceride, plasma free fatty acids (ffa), serum thyroxine (t4), corticosteroids, prolactin, growth hormone, immunoreactive insulin and urinary excretion of vma. t ...	1975	1202550
[investigation on the synthesis of 125i labelled triiodothyronine and thyroxine of high specific radioactivity (author's transl)].	the reaction conditions of the monoiodination of l-diiodothyronine and l-triiodothyronine by nonradioactive iodide have been investigated by separation of the reaction mixture on sephadex g-25 with sodium hydroxide 10 mmol/l as eluent and quantitative iodine estimation in the eluate. when labelling with 125i was performed under optimal conditions, a good yield of chromatographically pure l-triiodothyronine or l-thyroxine was obtained with a specific radioactivity between 2 and 3 ci/mg. the synth ...	1975	1202785
proceedings: studies on the specificity of action of bacteriophage t4 lysozyme.		1975	1205742
three-dimensional image reconstructions of the contractile tail of t4 bacteriophage.		1975	1206701
a mutation of the wobble nucleotide of a bacteriophage t4 transfer rna.		1975	1206705
the structure of bacteriophage t4 gene 12 protein.		1975	1206709
[evaluation of reliable pituitary suppression therapy through varying thyroid hormone dosage following total thyroidectomy due to a differentiated thyroid carcinoma].	the reliability of pituitary suppression by administration of 1-thyroxin (t4) or a combination of thyroxin and triiodothyronin (t3) of variable dosage following radical surgery for thyroid cancer in 13 patients was investigated by means of trh-test and measurement of serum hormone levels. in contrary to generally applied higher doses the results were able to demonstrate that 0.2 to 0.3 mg 1-thyroxin is a sufficient dosage for a complete suppression of tsh-stimulation. in the presence of obviousl ...	1975	1207280
prolactin and growth hormone synthesis: effects of perphenazine, alpha-methyltyrosine and estrogen in different thyroid states.	the effects of thyroid hormones on prolactin (prl) and growth hormone (gh) synthesis by the rat anterior pituitary gland were assessed in vitro. a marked reduction (84-87%) in the rate of h3-leucine incorporation into gh was evident 2-4 weeks after thyroidectomy, while incorporation into prl was 52-71% less than that measured in glands from intact rats. a single injection of t4 (200 mug/kg) administered to thyroidectomized (thx) rats 48 hr before sacrifice significantly increased incorporation i ...	1975	1208573
congenital abnormality of pituitary-thyroid axis in spontaneously hypertensive rats (shr) and stroke-prone rats (spr).	in an attempt to study whether tsh abnormality was genetically determined in shr and spr, plasma t4, t3, tsh and prolactin concentrations were measured in the animals with intervals of 1 to 3 months. hypertension was found in 6-month-old shr and spr, but it was not found in younger animals. in contrast, a decrease of plasma t3 and an increase of plasma tsh were found in 15-day-old shr. also, an increase of tsh was found in 1-month-old spr in spite of normal plasma t3 concentration. these abnorma ...	1975	1208577
the influence of fatty acids on serum thyroxine determination by competitive protein-binding radioassay.	thyroxine concentration, determined by competitive protein-binding radioassay (t4(d)), increased in serum stored at 20 degrees c and 37 degrees c. the increase correlated well with the increase in nonesterified fatty acids (nefa). in vitro addition of nefa to serum and in vivo increase in nefa induced by post-prandial heparin injection resulted in a proportional increase in t4(d) values. protein-bound iodine and thyroxine concentration, as determined by radioimmunoassay, were not influenced by s ...	1975	1209160
[res-o-mat-t4 test, tes-o-mat-etr test and thyroxine iodine as indicators of thyroid function in cattle].		1975	1209234
[tissue effects of intra-arterial chemotherapy in pelvilingual cancers and their lymphatic metastases (apropos of 47 cases)].	forty-seven patients with pelvi-lingual (mostly t4) cancers treated bae intra-arteri chemotherapy (i.a.c.) were studied histologiccaly to discover the effects of the chemotherapy. the results observed were classified according to a six degree histological scale ranging from total absence of tumoral regression to complete involution of the cancer. the product used, dosage, time lapse, clinical regression and histological formula were compared with the histological findings. the results were also ...	1975	1211757
reduction of serum lipids by means of etiroxate (liponorm).	ten out-patients with primary type iia hyperlipoproteinemia and a further 10 with types iib, iv, and v were administered with dl-alpha-methyl-thyroxine ethyl ester (etiroxate) (20 mg twice daily) for an average of 308 days. the aim of the study was to determine the effects of the drug on the cholesterol and triglyceride levels, tolerance and side-effects, particularly in coronary patients. the t4 values rose in all but one patient and fell again when the drug was discontinued. in type iia patien ...	1975	1212278
[deiodination in the kidney and thyroid function)].	in homogenate supernatants of kidneys of male rats the extent of deiodination of l-diiodotyrosine (l-djt) and l-thyroxine (l-t4) was investigated in dependence on the thyroid function (hypo- and hyperthyroidized) and also in dependence on age. in rats hypothyroidized by loading with methylthiouracil (mtu) or methimazol (mmi) the deiodination for l-djt and l-t4 was significantly reduced, in rats loaded with 40 mug t4 sc. for 10 days, the deiodination was significantly enhanced compared with untre ...	1975	1212993
serum thyroxine estimation: clinical evaluation of a modified semiautomated method and its ability for differential diagnosis of thyroid diseases.	a modified semiautomated microchemical method for determining serum thyroxine has been described. it consists of the isolation of t4 on a column of cation exchange resin dowex 50 w x 2 and of a fully automated determination of iodine in the ammoniacal eluate from the column after an acidic mineralization. the diagnostic value of the method has been evaluated on a small group of patients examined for suspected thyroid gland dysfunction, in comparison with a parallel determination of t4 by a cpba ...	1975	1212995
[phenotypic characteristics of temperature-sensitive mutants of bacteriophage t4 in gene 19].	the order of three thermosensitive mutations in gene 19 is determined and compared with the character of defective structures observed in an electrone microscope study of the lysates obtained in non-permissive and semi-permissive conditions. the degree of defectiveness of core polymerization increases if the mutation is located near the beginning of the gene. in one the combinations among these mutants the interallelic complementation is found. the mechanism of complementation is discussed on th ...	1975	1213286
total synthesis of a structural gene for the human peptide hormone angiotensin ii.	seven oligonucleotide chains containing between 6 and 11 nucleotide units were synthesized. the segments were phosphorylated by t4 polynucleotide 5'-hydroxyl-kinase and joined by t4 polynucleotide synthetase (atp) to give the double-stranded dna consisting of 33 base pairs. the dna sequence was deduced from the known peptide sequence according to the genetic code.	1975	1213674
nucleotide alterations in bacteriophage t4 serine transfer rna that affect the conversion of precursor rna into transfer rna.		1975	1214302
genetic map of the beginning of the riib cistron of bacteriophage t4.	in this report the herefore uncharacterized mutants at the beginning of the riib cistron of bacteriophage t4 have been connected to the main part of the map and have been shown not to be in any way unusual. the temperature sensitive mutant hd263 appears to be the earliest mutant in the cistron.	1975	1214778
[secondary structure of dna from t4 and t6 phages].	the secondary structure of nadna from e. coli t4 and t6 phages has been studied by the x-ray diffraction method. molecules of these dnas as well as t2 phage dna molecules contain hydroxymethylcytosine glucosylated at different position instead of cytosine. at high relative humidity these dnas are shown to exist in b-conformaion. as humidity decreases the transformation into t=conformation takes place in the t4 phage dna whereas in the t6 phage dna changes of secondary structure similar to b-t tr ...	1975	1214798
[study of dna melting in the region of the inversion of relative stability of at and gc pairs].	systematic data on the dependence of the melting curve parameters of dna from different organisms on the concentration of salt (c2h5)5nbr have been obtained. the melting curves were studied by spectrophotometric as well as by microcalorimetric methods. the dna melting range width is shown to pass through the minimum value delta0t = 0.6 +/- 0.1 degrees at the point of inversion of relative stability of at and gc pairs that corresponds to the concentration of (c2h5)4nbr equal to 2.9 +/- 0.1 m. thi ...	1975	1214815
[letter: progress in thyroid function testing. radiologic determination of thyroxine (t4-r)].		1975	1215157
dose-relation of thyroxine-induced changes in myocardial energy stores.	experimental hyperthyroidism was produced in guinea pigs by daily intraperitoneal injection of l-thyroxine (t4) in various doses (0.7, 0.35, 0.175, and 0.07 mg/kg/day) for 7 days. controls received solvent only. the following metabolites were determined in heart muscle (freeze-stop technique, enzymatic tests): pi, adenosine tri-, di-, and monophosphates (atp, adp, amp), creatine phosphate (cp), glucose 6-phosphate (g-6-p), fructose diphosphate (fdp), pyruvate, and lactate. thyroxine induced a do ...	1975	1215645
l-thyroxine and monoamine oxidase activity in the kidney and some other organs of the rat.	l-thyroxine (t4) (10,30 and 300 mug/kg. s.c. for 5 days) increased kidney monoamine oxidase (mao) activity and decreased that of the liver, using tryptamine as the substrate. these changes were dose-dependent. time-dependent changes were found with the 30 mug/kg dose. heart mao activity was increased by the high dose while small decreases were found with the lower doses. no changes were found in the whole brain, vasa deferentia, the thyroid or adrenal glands. hypophysectomized rats showed a 50% ...	1975	1215664
[the euthyreotic hypotriiodothyroninemia].	in 33 patients with various, mainly severe diseases a subnormal triiodothyronine concentration (t3) and a normal thyroxin concentration (t4) were found. in addition to t3 and t4, the plasma tsh levels and t3 binding capacity were determined. in no case did the patient's history or clinical findings afford evidence of hypothyroidism. diminished conversion of t4 to t3 is discussed as a possible cause of the inappropriately depressed t3 levels.	1975	1215959
[recombination in amber-mutants of bacteriophage t4b. i. recombination and complementation in phage t4 amber-mutants].	in studying intergenic and intragenic complementation in amber mutants in genes of phage t4 controlling the synthesis of phage tail fibres the data have been obtained indicating the dependency of the results of complementation tests on those of crosses of respective markers. the results obtained show that in complementation of amber mutants of phage t4 the phage yield varies widely and depends on the location of markers on the phage genetic map.	1975	1218721
[effect of palladium and platinum salts on bacteriophage t4 and its isolated dna].	the effect of palladium and platinum salts (k2pdcl4, k2ptcl4) on bacteriophage f4 and its isolated dna in genetic transformation is investigated. both compounds efficiently inactivated the phage and decreased the transforming activity of donor dna. the palladium salt exhibited the higher activity. the palladium compounds inhibited the transforming activity of native donor dna to a greater degree. no difference was observed in the degree of inactivation of the transforming activity of native and ...	1975	1218722
differential synthesis of proteins by t4 phage & its head protein amber mutant.		1975	1218878
[fifty cases of penile tumors treated at the claudius-regaud center from 1958 to 1973].	from the study of this series of 50 patients suffering from cancer of the penis, and in particular from the comparison of two successive groups, several facts stand out which determine our present attitude: -subjects suffering from cancer of the penis, at least in our area, are aged, fragile people, whose cause of death, in more than half of the cases, was not the penile cancer. proposable therapeutic methods must take this specific background into account; -tumoral and glandular indications mus ...	1975	1219091
[serum level of thyroxin-binding globulin under oral contraception (author's transl)].	the use of oral contraceptives results in an increase in thyroid-binding-capacity in conformity with a change of results by t3/t4-tests. using laurell-electrophoresis and an anti-serum produced by behringwerke the determination of the thyroid-binding-globulin (tbg) in serum is easy to handle. tbg was determined in serum from 137 women using oral contraceptives for more than 3 months. the apllication of oral contraceptives is followed by a statistically significant increase in the tbg level in se ...	1975	1219185
[tsh and t4-level in newborn infants with congenital struma].		1975	1219466
normal values of thyroxine and triiodo-thyronine retention in the rat. valores normais de tiroxina e retenção de triiodo-tironina em ratos.	the authors report the results from murphy and pattee's method 9, 10 1964, 1966 for t4 and those from hamolsky et al.'s method 5 1957 for percent retention of t3 as applied to the normal rat serum. for that purpose, 32 adult wistar rats, of both sexes, kept at the laboratory environment and fed with usual diet were used. blood samples were taken after noon directly from the heart, without anesthesia. the analysis showed a t4 value of 4.20 +/- 0.92 mug/100 ml of serum (both sexes). the percentual ...	1975	1219925
[studies in the diagnosis of breast disease (author's transl)].	the relative diagnostic accuracy of clinical examination, mammography, thermography and ultrasound was investigated in a comparative study. mammography proved to be the most accurate diagnostic method, followed by clinical examination, thermography and ultrasound. whereas in cases of advanced cancer (t2 to t4 tumour diameter greater than 2 cm) the correct diagnosis was made most reliably by clinical examination, mammography was superior to all other procedures in t1 tumours and in impalpable tu ...	1975	1220258
[serum concentration of triiodothyronine in autonomous thyroid gland adenoma].	serum concentration of t3 was measured in 14 patients with autonomous thyroid adenoma using a chromatographic method. 9 patients had increased levels of t4, 5 were normal. in both groups serum-t3-concentration was significantly elevated. in patients with normal t4-values hypertriijodothyroninemia seems to be the biochemical base for clinical signs of hyperthyroidism. the pathogenetical importance of elevated t3-levels is discussed for t3-secretion and t4/t3 conversion.	1975	1220518
[absorption and pharmacokinetics of large doses of 1-thyroxine in man (author's transl)].	in recent years suppression tests with a single dose of 3 mg 1-thyroxine (t4) and weekly doses of 1 mg t 4 in the treatment of hypothyroidism have been put to clinical trial and the lack of side-effects of such high doses (5 to 15 x daily requirements) was stressed. hence, it was decided to study the absorption of 1-t-4 from the gastrointestinal tract and its metabolism in euthyroid patients. doses from 250 to 2500 mug t 4, mixed with 250 muci 131i-thyroxine were given to 10 patients. after the ...	1975	1221631
[deiodase activity in rats following an overdose of thyroid hormone].	the l-dit and l-t4 deiodinating activity in supernatants from liver or kidney homogenates of normal rats or rats loaded with t4, t3 or tsh, was investigated. deiodination of l-dit occurs in liver supernatants twice as much as in liver, referring to mg of protein of g of tissue, while the deiodination of l-t4 seems rather equal. the over all rate of deiodination, however, reaches in liver for l-dit the threefold, for l-t4 the fivefold value, as compared with kidney. short periods of loading do no ...	1975	1222735
[radioimmunologic determination of plasmatic triiodothyronine. verification of operative parameters; 1st clinical results].	the experimental determination of the operative parameters of two radioimmunoassay (ria) systems for the determination of triiodothyronine (t3) level directly in serum is described. the two systems differ both for the agents blocking the aspecific t3-serum protein bindings (sulphonic acid, 8-aniline, 1-naphtalene in borate buffer: bor-ans-ria and merthiolate in phosphate buffer: po4-merth-ria) and for the methods adopted for compensating the aspecific serum interferences (t3,4 free serum for bor ...	1975	1223940
the influence of dietary iodine on lithium blood level, serum t4 and thyroid gland weight.	lithium is both antithyroid and goitrogenic. the relationship between lithium and iodine has been assessed in rats fed diets containing different iodine content. chronic lithium treatment caused increase in thyroid gland weight in animals on low and high iodine diets. a reciprocal relationship surfaced between serum lithium levels and dietary iodine content. animals on low-iodine diet had lower serum lithium levels. the data support other evidence that lithium likely inhibits organification of i ...	1975	1224012
[correlations between clinical control and histopathologic findings in breast cancer after irradiation and radical mastectomy (author's transl)].	154 cases of breast cancer (30 t1-52; 124 t3-54) treated by irradiation followed by radical mastectomy were subjected to histological revision in order to check the efficacy of radiotherapy in the local control of cancer and to seek correlations between the histological findings and the clinical course of the disease. the tumor disappeared completely only in 24% of cases, the percentage being higher in the initially smaller tumors; the axillary lymph nodes showed disappearance of the metastases ...	1975	1224390
low dose irradiation in advanced tumours of head and neck.	a material of 19 patients with t3 and t4 tumours of the mouth and pharynx was treated by fractionated low dose rate irradiation. regression occurred in a large proportion of tumours but at the expense of a high rate of necrosis.	1975	1224991
pre-operative short intensive radiation therapy of t3-t4 laryngeal carcinoma.	a group of 33 patients with t3-t4 laryngeal carcinomas received in the period 1968 to 1972 pre-operative irradiation with 60co followed by surgery. a total dose of 2 500 rad was given in 5 equal sesssions. the tumour free survival rates of these patients are compared to those of a comparable group of 57 patients receiving radiation therapy only. the overall results in the pre-operative group (64%) are superior to those in the group irradiated only. in glottic tumours the results were similar in ...	1975	1224993
myocardial high energy phosphates in experimental thyrotoxicosis in guinea pigs.	experimental thyrotoxicosis was produced in guinea pigs by daily intraperitoneal injection of 0.7 mg/kg thyroxine (t4) or 0.15 mg/kg triiodothyronine (t3), as shown by an increase of pbi over 50 gamma percent, weight reduction of 20--30 percent, and increase in heart rate of 30--50 percent. control animals received solvent only. metabolites were determined in heart muscle on the 1st, 3rd, and 7th day using freeze-stop technique and enzymatic methods: creatine phosphate (cp), atp, adp, amp, gluco ...	1975	1226434
further studies on prostaglandins. iii. effect of prostaglandins on thyroid activity as assessed by 125i.	the effect of crude prostaglandins extraced from prostate glands of camels as well as that pge1, on the thyroid activity of male immature boskat rabbits, was investigated. the iodinated amino acid fractions (t1, t2, t3 and t4) in the serum of the injected groups were determined in control and treated animals. cytological work on sections of the thyroid glands of the treated and control rabbits were performed to study the effect on subcellular level. the results indicate that both crude and pure ...	1975	1227829
[effect of mutant rna polymerase on spontaneous mutagenesis in bacteriophage t4].	the frequency of spontaneous reversion in r-mutants of phage t4 is determined in order to study the influence of mutant rna polymerase on the level of spontaneous mutations in phage t4. the rate of spontaneous mutagenesis in the phage is found to be increased in strains with mutant rna polymerase.	1975	1228060
[the influence of proteins bound to single-stranded dna on rna and poly(a) synthesis. i. antibodies against dna].	the influence of antibodies to dna of t4 and spoi phages as well as to dna of e. coli on rna and poly(a) synthesis by e. coli rna polymerase has been studied. antibodies to the phage dna specifically inhibit rna and poly(a) synthesis by 30 to 60% only in case of homologous dna, the stage of elongation being inhibited predominantly. a comparison between the effects of the antibodies upon different stages of rna synthesis on the native dna and on the synthetic oligonucleotide template (dt)12 allow ...	1975	1228470
inactivation of hapten-modified bacteriophage by antibody: increased sensitivity of the assay in media containing polyethylene glycol.	inactivation of dinitrophenylated bacteriophage t4 by porcine and rabbit anti-dinitrophenyl (dnp) antibodies was studied by the complex inactivation methods. the inactivation titers increased 20-30 times when polyethylene glycol, mol. wt. 6000, was added to the incubation media. consequently, the detection of dinitrophenylated serum albumin with the modified bacteriophage could be carried out with higher sensitivity upon addition of polyethylene glycol.	1975	1234056
bacteriophage t4 baseplate components. i. binding and location of the folic acid.	two different proteins with high affinities for the pteridine ring of folic acid have been used to determine the location of this portion of the folate molecule in the tail plate of t4d and other t-even bacteriophage particles. the two proteins used were (i) antibody specific for folic acid and (ii) the folate-binding protein from bovine milk. both proteins were examined for their effect on various intact and incomplete phage particles. intact t2h was weakly inactivated by the antiserum but not ...	1975	1238580
nyctohemeral and sex-related variations in plasma thyrotropin, thyroxine and triiodothyronine.	tsh, t4, and t3 were measured by radioimmunoassay in plasma samples obtained from 77 young adult male and 114 female rats fed a purina high-iodine diet and maintained in an isolated room, 2-4/cage, at 24 +/- 1 c with light from 0600-1800 h. in one experiment, 7 male and 7 female rats were decapitated every 3 h for 30 consecutive h and trunk blood was collected. there was a clear nyctohemeral rhythm of plasma tsh in both sexes characterized by a zenith at 1200 h and a nadir between 1800 and 2100 ...	1975	1239368
opposite effects of dexamethasone on serum concentrations of 3,3',5'-triiodothyronine (reverse t3) and 3,3'5-triiodothyronine (t3).	dexamethasone, 2 mg every 6 hours for 4 doses, was given to 4 hypothyroid patients receiving treatment with synthetic thyroxine (t4) and to 8 untreated hyperthyroid patients with graves' disease, and serum concentrations of thyroid hormones were measured by radioimmunoassays. serum concentration of 3,3'5'-triiodothyronine (reverse t3, rt3) increased appreciably within 8 hours after the first dose of dexamethasone, was maximum at 24-32 hours after beginning dexamethasone, and remained elevated fo ...	1975	1242390
solitary hyperfunctioning thyroid adenoma in a child. elevated t3 and t4 levels with normal tsh level.		1975	1242753
[variation of the thyrotropin-releasing-hormone (trh) stimulated thyrotropin (tsh) response in comparison with the tyhroid-gland-suppression test and the triiodothyronine (t3) and thyroxine (t4) blood levels in the so-called euthyroid endocrine ophthalmopathy].	21 patients with active signs of euthyroid graves' disease were given 400 mug thyrotropin-releasing hormone (trh) i.v. all subjects with unresponsiveness to trh had a nonsuppressible thyroidal 131i-uptake. on the basis of serum total t3 14 patients were hyperthyroid, 2 more had an elevated value of free t3. 4 patients with normal total t3 and nonsuppressible 131i-uptake were unresponsive to trh, in 2 of them the free t3 fraction was elevated, however. 4 subjects with nonsuppressible 131i-uptake ...	1975	1243821
high incidence of chronic lymphocytic thyroiditis in apparently healthy school children: epidemiological and clinical study.	an epidemiological survey on the incidence of juvenile chronic lymphocytic thyroiditis was performed in 10,220 apparently healthy school children in ishikawa district, japan. the subject of present study included 6,244 school children (2,831 boys and 3,413 girls, ages 6-18 yrs.) in kanazawa city and 3,976 children (2,055 boys and 1,921 girls, ages 6-18 yrs.) in wajima city. the first group was selected as a representative of urban area and the second group as that of seaside area. children who h ...	1975	1243913
[clinical studies on autoimmune mechanisms of the thyroid. part 2. studies on measurements of serum thyroblobulin concentrations in patients with various thyroid diseases (author's transl)].	the present report deals with the measurement of serum thyroglobulin (tg) in various thyroid disorders by using radioimmunoassay (ria). the results were as follows: 1) a specific and simple solid-state ria for the measurement of tg in human serum was used. this system was a direct ria using plactic cups coated with crude anti-thyroglobulin antibodies (anti-tg) and 125i labeled purified anti-tg. the purification of anti-tg was performed by affinity chromatography using tg-sepharose, as an immunoa ...	1975	1243914
reciprocal changes in serum concentrations of 3,3',5-triiodothyronine (t3) in systemic illnesses.	serum concentrations of 3,3',5'-triiodothyronine (reverse t3, rt3) were measured in adult patients with several systemic illnesses whose serum total and/or free t3 were low, serum total t4 was low or normal, and free t4 was either normal or elevated. the mean serum rt3 was 76, 46, and 77 ng per 100 ml in patients with hepatic cirrhosis, chronic renal failure, and acute febrile illnesses, respectively; the values in patients with hepatic cirrhosis and acute febrile illness were significantly high ...	1975	812882
klinefelter's syndrome: examination of thyroid function, and the tsh and prl responses to thyrotropin-releasing hormone prior to and after testosterone administration.	thyroid function and prolactin (prl) responsiveness to thyrotropin-releasing hormone (trh) were examined in 6 patients with klinefelter's syndrome prior to and after therapy with testosterone. the thyroid function tests, including serum triiodothyronine (t3), thyroxine (t4), thyroxine binding globulin (tbg), resin t3 uptake (rt3u), radioactive iodine uptake (raiu), thyrotropin (tsh) stimulation and the tsh response to trh were normal during both periods of study. testosterone treatment had no si ...	1975	812883
replication and recombination of gene 59 mutant of bacteriophage t4d.	after infection of escherichia coli b with phage t4d carrying an amber mutation in gene 59, recombination between two rii markers is reduced two- to three-fold. this level of recombination deficiency persists even when burst size similar to wild type is induced by the suppression of the mutant dna-arrest phenotype. in the background of two other dna-arrest mutants in genes 46 and 47, a 10- to 11-fold reduction in recombination is observed. the cumulative effect of gene 59 mutation on gene 46-47 ...	1975	1107596
effects of bacteriophage t4-induced modification of escherichia coli rna polymerase on gene expression in vitro.	after t4 bacteriophage infection of e. coli a complex series of events take place in the bacterium, including gross inhibition of host transcription and discrete changes in the classes of the genes of t4 that are transcribed. accompanying these changes in the pattern of transcription one finds t4-induced changes in the rna polymerase (ec 2.7.7.6; nucleosidetriphosphate:rna nucleotidyltransferase). the effects of modified polymerase on transcription can be advantageously analyzed in a dna-directe ...	1975	1108008