Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [association of thyroxine with subcellular fractions of rat liver and adenohypophysis in vitro (author's transl)]. | after incubation of thyroxine (t4) labelled with 125i with liver and adenohypophysial homogenate and differential centrifuging into the "nuclear" fraction (600 g), "mitochondrial" (10 000 g), "microsomal" (105 000 g) fraction and cytosol the authors found a marked preference of association of t4 with mitochondria of rat adenohypophyses; the mitochondrial fraction of rat liver was not preferred. the effect of oestrogens was manifested in addition to the usual increase in weight of the adenohypoph ... | 1975 | 1111056 |
| troponin and parvalbumin calcium binding regions predicted in myosin light chain and t4 lysozyme. | a computer search of available protein sequences and structures suggests that bacteriophage t4 lysozyme contains one region and that rabbit myosin light chains contain three regions similar, and supposedly homologous, to the calcium binding region of carp muscle calcium binding parvalbumin. | 1975 | 1111094 |
| anti-dinitrophenyl antibody production in strain 13 guinea pigs fed or sensitized with dinitrochlorobenzene. | guinea pigs fed dinitrochlorobenzene (dncb) in corn oil become unresponsive tosensitization by later percutaneous applications of dncb in olive oil. sera taken from animals after feeding dncb or after feeding and intradermal stimulation with dncb contained no antibody detectable with 2,4-dinitrophenyl (dnp)-labeled t4 bacteriophage; if any antibody was present, its concentration was below 2 mu g/ml. guinea pigs fed dncb and then immunized with dnp on a foreign carrier, keyhole limpet hemocyanin ... | 1975 | 1112982 |
| the 3'-terminal nucleotide sequences of adenovirus types 2 and 5 dna. | short nucleotide sequences at the 3'-termini of adenovirus types 2 and 5 dna have been determined using t4 dna polymerase as described by p. t. englund (1972). the terminal sequences of both serotypes appear to be completely identical. both molecular ends of type 2 as well as of type 5 dna terminate with the sequence ...pcpc...pgpaptpg3', consistent with the presence of an inverted terminal repetition in adenovirus dna. | 1975 | 1113373 |
| t4 dna polymerase (gene 43) is required in vivo for repair of gaps in recombinants. | experiments with a mutant of t4, tsl97, temperature sensitive for gene 43, showed that t4 dna polymerase was necessary in vivo to repair gaps in recombinant molecules. cscl density gradient experiments showed that molecular recombinants were not repaired when the t4tsl97-infected cells were shifted to 42 c after replication and recombination had taken place. repair was almost complete when the same procedure was followed with the wild-type t4, or when the t4tsl97-infected cells were incubated at ... | 1975 | 1113374 |
| optimal treatment for the technically resectable squamous cell carcinoma of the supraglottic larynx. | the charts of 431 patients with squamous cell carcinoma of the supraglottic larynx observed at the m.d. anderson hospital between january, 1954, and june, 1971, were analyzed. this study is concerned with those patients who had a technically resectable lesion. emphasis is directed to the analysis of the effectiveness of primary irradiation instead of partial laryngectomy for those lesions which are technically suitable for a partial resection and to define the groups of patients which are best t ... | 1975 | 1113591 |
| characterization of a new class of deletions of the d region of the bacteriophage t4 genome. | 1975 | 1114706 | |
| measurement of plasma and tissue triiodothyronine concentration in the rat by radioimmunoassay. | this paper reports a radioimmunoassay method for triiodothyronine (t3) and the application of this assay to the study of plasma and tissue t3 concentration in the rat. several antisera formed to a t3-bovine fibrogen complex in guinea pigs and t3-thyroglobulin complex in rabbits are shown to have low or no cross reactivity with t4, monoiodotyrosine, diiodotyrosine, tetraiodothyroacetic acid (tetrac) and reverse t3. cross reactivity with t3 derivatives, triiodothyroacetic acid (triac), and triiodo ... | 1975 | 1116483 |
| thyrotrophin concentrations in the plasma of cattle, sheep and foetal lambs as measured by radioimmunoassay. | a radioimmunoassay technique for measuring the thyrotrophin (tsh) concentration of sheep and cattle plasma is described. the sensitivity of the assay allowed the measurement of 1-50 ng tsh/ml unextracted plasma. cross-reaction with ovine luteinizing hormone, prolactin and growth hormone was very low. the average recovery of added tsh was 103 plus or minus 4-1 (s.e.m.)% and the between-assay coefficient of variation was 13-8%. the normal plasma tsh levels of sheep and cattle were approximately 2- ... | 1975 | 1117244 |
| novel rii duplications in bacteriophage t4. | the properties of two rii complementation heterozygotes (d5b and d7a) of bacteriophage t4 are described. these strains are characterized by their stability, each forming less than 10-3 r segregants among their viable progeny, and by their segregation of only one of the two parental types. no increase in r progeny was found on crossing d7a or d5b with t4r+, indicating that the duplications in these strains are not separated by an essential region of the phage genome. both d5b and d7a from h-2+/h- ... | 1975 | 1117484 |
| correlation of serum triiodothyronine (t3) and thyroxine (t4) with biologic effects of thyroid hormone replacement in propylthiouracil-treated rats. | to study the role of t4 to t3 conversion in the biologic action of t4, thyroidectomized, hypothyroid rats were given subcutaneous t4 (0.8 or 1.6 mug/100g/day) with or without intraperitoneal propylthiouracil (ptu) (1 mg/100g/day). rats were killed after 5, 10, 12, or 15 days of treatment and serum t3 and t4 levels were correlated with serum tsh, liver mitochondrial alphagpd activity and weight gain. in rats killed at 5 days, ptu treatment resulted in higher serum t4, lower serum t3, and a marked ... | 1975 | 1117844 |
| serum thyroid hormone and thyrotropin levels in subjects from endemic goiter regions of new guinea. | we studied serum thyroid hormone and thyrotropin (tsh) levels in subjects from two regions (nomane and karkar) of new guinea where endemic goiter and/or iodine deficiency are prevalent. the results of the studies in 285 patients from nomane indicated subnormal serum t4 (mean plus or minus sd, 6.5 plus or minus 2.8 vs 8.4 plus or minus 2.0 mug/1ll ml, for normal americans, p less than 0.001), supranormal serum t3 (161 plus or minus 51 vs 126 plus or minus 33, ng/100 ml, p less than 0.001), supran ... | 1975 | 1117981 |
| determination of recognition sites of t4 rna ligase on the 3'-oh and 5' -p termini of polyribonucleotide chains. | 1975 | 1118037 | |
| negative cooperativity in the binding of thyroxine to human serum prealbumin. preparation of tritium-labeled 8-anilino-1-naphthalenesulfonic acid. | the binding of thyroxine (t4) and 8-anilino-1-naphthalenesulfonic acid (ans) to human serum prealbumin was measured by equilibrium dialysis at ph 7.4 in 0.05 m phosphate-0.10 m nacl at 25 degrees. the data were analyzed for the binding constants based on equations for (1) two independent sites and (2) two identical sites with negative interaction. evaluation by the independent site model gave the following association constants: for t4 binding, kt1 = 1.0 x 10-8 m-1, kt2 = 9.5 x 10-5 m-1; for ans ... | 1975 | 1120103 |
| dna-dependent rna polymerase from pseudomonas bal-31. i. purification and properties of the enzyme. | the dna-dependent rna polymerase from pseudomonas bal-31, the host for bacteriophage pm2, has been purified 154-fold using differential centrifugation, chromatography on deae-cellulose, ammonium sulfate precipitation, and sucrose gradient centrifugations at low and high ionic strength. the resulting enzyme is free of enzyme activities which could interfere with transcription studies and is greater than 85% pure as judged by polyacrylamide gel electrophoresis. like other bacterial rna polymerases ... | 1975 | 1120104 |
| direct effects of thyroid hormones on bone marrow erythroid cells of rats. | a stimulatory effect on bone marrow cellularity was observed in normal and nephrectomized rats continuously infused with t3 and t4. results of bone marrow studies are expressed in absolute numbers of total nucleated erythroid cells per milligram of femoral marrow at the beginning and after 8 hr of continuous intravenous infusions. administration of t3 and t4 to nephrectomized rats produced a marked and significant increase in total erythroid cells counted. after differential analyses of the nucl ... | 1975 | 1120189 |
| conversion of thyroxine (t4) and triiodothyronine (t3) and the subcellular localisation of the converting enzyme. | 1975 | 1120365 | |
| cycloleucine transport in isolated rat thymocytes: in vitro effects of triiodothyronine and thyroxine. | thymocytes obtained from suckling or young adult rats were used as a model system to study the action of thyroid hormones in vitro. in this tissue, l-triiodothyronine (t3) increased the uptake of the non-metabolizable amino acids, alpha-aminoisobutyrate and cycloleucine. a detectable effect of t3 on the uptake of cycloleucine was seen at a concentration of 0.1 mum and maximum effects were seen at 20 mum. thyroxine (t4) also increased cycloleucine uptake with about one-third the potency of t3, an ... | 1975 | 1120468 |
| effect of physical training on the metabolism of thyroid hormones in man. | the effect of a 6-wk program of physical training (track running) on the peripheral metabolism of thyroxine (t4) and triiodothyronine (t3) was evaluated in a group of 11 men. measurements were made of hormone turnover, urinay and fecal clearances, plasma hormone concentrations, and hormone binding by plasma proteins in all subjects before and after training, after training, metabolic clearance of t3 was increased 8.5% above the pretraining level due to an increased deiodinative clearance of this ... | 1975 | 1120742 |
| assessment of the degradation of thyroid hormones in man during bed rest. | the effect of bed rest on the absolute turnover rates of thyroid hormones, thyroxine (t4) and triiodothyronine (t3), was evaluated in man. bed rest resulted in physical deconditioning, measured by a decreased rate of maximal oxygen consumption; physical deconditioning was associated with no significant alterations in the metabolic clearance of t4 and t3. plasma concentrations of these iodothyronines were not changed as a result of bed rest. absolute hormone turnover rates were similar in control ... | 1975 | 1120743 |
| effects of p-fluorophenylalanine on the induction of mutations in bacteriophage t4. ii. nitrous acid mutagenesis. | the effects of rho-fluorophenyalanine (pfpa), an analogue of phenylalanine (phe), on the potency and the specificity of nitrous acid (na) mutagenesis in the gamma system of bacteriophage t4 were measured. (a) forward mutation. the frequencies of na-induced gamma mutants were approximately doubled when mutagenized phage infected e. coli b in the presence of the analogue compared to controls where phe was substituted for pfpa, or where no amino acid was added. the spontaneous forward mutation freq ... | 1975 | 1121316 |
| the iodine requirement and influence of iodine intake on iodine metabolism and thyroid function in the adult beagle. | various aspects of iodine metabolism were studied in adult beagles maintained at iodine intake levels ranging from 480 to 20 mug/day. on the basis of changes in radioiodine metabolism, the minimum daily iodine requirement of the adult beagle was found to be 140 mug. although striking changes were observed in radioiodine metabolism when iodine intake was reduced to 90mug/day, serum t4 and t3 levels were unaffected. marked reductions in serum t4 occurred in dogs restricted to an iodine intake of 5 ... | 1975 | 1122886 |
| mechanism of the mutagenic action of hydroxylamine. viii. functional properties of the modified adenosine residues. | the action of o-methylhydroxylamine in vitro upon some amber and rii ochre mutants of t4 phage leads to a considerable increase in the frequency of reversions and conversions of ochre and opal mutants presumably due to a yields g transitions. the transitions seem to be caused by modification of the adenine bases of the genome to give n-6-methoxyadenine which has equivocal (a- and g-like) specificity. however, the functional activity of n-6-methoxyadenosine residues in the rna polymerase system ... | 1975 | 1125310 |
| attempts to detect agrobacterium tumefaciens and bacteriophage ps8 dna in crown gall tumors by dna-dna-filter hybridization. | a systematic study of the dna-dna-filter reaction is presented which measures its ability to detect small amounts of simple dna (bacterial or bacteriophage) in model mixtures of dna immobilized on filters. saturation curves show qualitatively that significant binding occurs when there is 10% agrobacterium tumefaciens dna on the filter but not 1%. ps8 bacteriophage dna is detectable at a level of 0.1%. true saturation is not attained in the bacterial dna reaction : radioactivity bound represents ... | 1975 | 1125316 |
| semi-automated radioimmunoassays for total serum thyroxine and triiodothyronine. | single stage semi-automated radioimmunoassays for total serum thyroxine (t4) and triiodothyronine (t3) are described which employ an automatic pipetting station, automatic gamma counter, and a programmable calculator with paper tape reader and printing facility. both assays require only a small volume of unextracted serum, and are specific and sensitive. their sample capacity, precision, speed, and cost are comparable with the measurement of serum protein-bound iodine. both assays therefore have ... | 1975 | 1126029 |
| the effect of food deprivation of the peripheral metabolism of thyroxine in rats. | starvation depresses thyroid gland function. in addition, the peripheral turnover of thyroxine (t4) is reduced, in part due to decreased fecal elimination of t4. the present studies were performed to determine if starvation also affects the deiodinative pathway for t4 degradation. rats were isotopically equilibrated with daily injections of exogenous [131-i]t4 while endogenous thyroidal t4 secretion and concentration of iodide were blocked with kcl04. following a period of equilibration, either ... | 1975 | 1126320 |
| studies of mutations in t4 control genes 33 and 55. | available mutations in transcriptional control genes 33 and 55 of coliphage t4 have been examined. by complementation analysis and map position, 15 mutants (13 in t4d, 2 in t4b) have been shown to lie in gene 33 and 6 (5 in t4d, 1 in t4b) in gene 55. according to patterns of suppression and recombination, these mutants define three distinct amber sites in gene 33 and also three distinct amber sites in gene 55. all of these mutations are true amber mutations, in apparent contrast to some traditio ... | 1975 | 1126625 |
| the stimulation of human prolactin secretion by 3-iodo-l-tyrosine. | oral administration of a single 1 g dose of mit to 10 normal male and female subjects resulted in a rise in serum prolactin in each subject. the mean peak level of serum prolactin attained by the 10 subjects was 36.3 plus or minus 7.9 ng/ml which was highly significantly elevated (p smaller than 0.0005) above the mean basal level of 5.3 plus or minus 1.0 ng/ml. while there was no significant difference between the basal serum prolactin levels of male and female subjects, the mean peak level atta ... | 1975 | 1127080 |
| pituitary secretion of free alpha and beta subunit of human thyrotropin in patients with thyroid disorders. | utilizing sensitive and specific radioimmunoassays, serum concentrations of human thyrotropin (htsh), the immunologically common alpha subunit of the glycoprotein hormones, and the specific beta subunit of htsh ( htsh-beta) have been measured in normal individuals, in patients with primary hypothyroidism, and in patients with other disorders of thyroid function before and after intravenous administration of thyrotropin releasing hormone (trh). in 29 normal individuals htsh-beta was not detectabl ... | 1975 | 1127093 |
| metabolism of thyroxine and triiodothyronine in patients with chronic granulomatous disease. | normal human leukocytes can degrade thyroid hormones in vitro especially during phagocytosis. to test whether the leukocyte system plays a significant role in hormone economy in vivo, studies of the metabolism of labeled t4 and t3 granulomatous disease, whose leukocytes had earlier been shown to have a reduced ability to degrade the thyroid hormones. hormone turnover rates in the 3 subjects were similar to those reported for normal children by various investigarors. also, normal ratios between f ... | 1975 | 1127096 |
| triiodothyronine turnover in hospitalized psychogeriatric patients. | in a group of 12 hospitalized psychogeriatric patients, the fractional triiodothyronine (t3) turnover rate was significantly higher than in healthy control subjects of the same age, whereas the thyroxine (t4) turnover rate and other aspects of pituitary-thyroid function were virtually similar in both groups. despite the presence of manifestations of sympathetic overactivity or hypermetabolism, the patients did not have true hyperthyroidism, as verified by the laboratory findings and the results ... | 1975 | 1127197 |
| prognosis of squamous-cell carcinoma of the gums with cytologically verified cervical lymph node metastases. | a follow-up study was made of 125 patients with carcinoma of the gums treated from 1958 to 1969. twenty-one of these patients with cytologically verified metastases of the neck at first visit were specially studied. the primary tumour was classified as t1 in three patients, t2 in one, t3 in nine, and t4 in eight patients. twelve patients received radiotherapy, nine radiotherapy and surgery. the five-year determinate survival rate was 7 per cent in this group compared with 41 per cent for patient ... | 1975 | 1127333 |
| effects of gentamicin on the thyroid function of rats. | the effect of gentamicin (gm) on the thyroid function was investigated in relation with its stimulating action to the growth of rats at a very small dose level. the results may be summarized as follows: (1) changes in 131-i-uptake of the thyroid after administration of gm were unremarkable. the change in wet weight of the thyroid was also unremarkable. therefore, gm may not affect the thyroid itself. (2) gm-treated rats clearly showed a decrease in 131-i-triiodothyronine resine sponge uptake (tr ... | 1975 | 1127832 |
| the formation of a complex between the membrane structures of the mitochondria and dna of nonmitochondrial origin. | the ability of decomposed rat liver mitochondria, stripped of their outer membrane (fracton c) to bind 14c-dna of phage t4 was demonstrated. the bound phage dna is 10-15% resistant to treatment of fraction c with dnaase i. treatment of fraction c with sarcosyl (final concentration 0.1%) and centrifugation in a linear sucrose gradient (20-50%) permits the detection of a label in the mt-dna-membrane complex, isolated at 35% sucrose. further fractionation of the membrane complex on sepharose 4 b pr ... | 1975 | 1128504 |
| [needle puncture of the intervertebral disk foci in the bacteriological diagnosis of infectious spondylodiscitis. technic and results]. | clinical, laboratory, and radiological data do not make possible a certain diagnosis of infectious spondylitis: errors are thus possible, even frequent, and are harmful to the patient. in order to ensure a certain diagnosis and to avoid exploratory surgery as far as possible, the authors propose systematic needle puncture of the inververtebral disk--a technique that is simple and inoffensive to carry out in all disks below t4, and that, in a series of 18 cases, gave a success rate of 2 out of 3 ... | 1975 | 1129576 |
| the autonomous nodule of the thyroid: correlation of patient age, nodule size and functional status. | in light of new techniques for measuring circulating thyroid hormones and for studying the thyroid gland, we present our experience with 35 patients with solitary autonomous nodules of the thyroid to define more precisely the clinical course of patients with this disorder. the patients ranged in age from 19 to 80 years and 31 of the 35 were female. younger patients were generally euthyroid and sought attention because of a thyroid mass; virtually all older patients were hyperthyroid. eighteen ha ... | 1975 | 1130435 |
| a critical review of the management of bladder neoplasia using a modified form of helmstein's pressure therapy. | 30 patients with proven bladder malignant disease have been treated by helmstein's hydrostatic pressure technique. massive necrosis of tumour tissue has been shown to occur at cystoscopy, cytologically and on histological examination. double contrast cystography can indicate reduction in tumour size within 2 weeks of treatment haematuria is usually stopped with diminution of frequency and dysuria. this treatment is suitable for large bulky t1 tumours and advanced t4 growths where palliation is t ... | 1975 | 1131497 |
| [radioimmunological estimation of triiodothyronine in plasma. diagnostic value in an area with endemic goitre (author's transl)]. | in 896 patients from a low-iodine area the results of the radioimmununological triiodothyronine estimations in plasma were compared with the clinical and radioisotope findings of thyroid function. it was superior to the total thyroxine estimation (t4-test) in the differential diagnosis of hyperthyroidism. in euthyroid goitre the mean triiodothyronine concentration was significantly higher than in normal persons whereas the plasma thyroxine levels at the same time were significantly lower. only i ... | 1975 | 1132323 |
| circulating 3,3', 5'-triiodothyronine (reverse t3) in the human newborn. | serum concentrations of 3,3',5'-triiodothyronine (reverse t3 rt3), 3,3',5-triiodothyronine (t), and thyroxine (t4) were measured in cord blood and invenous blood samples obtained between 2 h and 30 days of postnatal life from healthy full-term newborn infants. the mean serum rt3 concentration of (mean plus or minus se) 151 plus or minus 12 ng per 100 ml in 18 cord blood samples was significantly higher than the level (41 plus or minus 2 ng per 100 ml) in 27 normal adult sera; the corresponding m ... | 1975 | 1133163 |
| estimation of thyroxine and triiodothyronine distribution and of the conversion rate of thyroxine to triiodothyronine in man. | studies on peripheral metabolism of simultaneously administered 125-i-labeled l-thyroxine ([125-i]t4) and 131-i labeled l-trilodothyronine ([131-i]t3) were performed in five normal subjects, in four patients with untreated hypothyroidism, and in 3 hypothyroid patients made euthyroid by the administration of t4. the fractional turnover rate (lambda 03) of thyroid hormones irreversibly leaving the site of degradation and the volumes of pool 1 (serum v1) of pool (interstitial fluid, v2), and of p ... | 1975 | 1133178 |
| effect on isoflurane anaesthesia and surgery on thyroid function in man. | the effect of isoflurane (forane) anaesthesia and surgery on thyroid function was investigated in nine male patients. isoflurane anaesthesia alone for 30 minutes prior to the start of surgery increased plasma triiodothyronine uptake (t3u) and thyroxine (t4) level by 18 and 20 per cent, respectively. free thyroxine index (fti) values in blood increased by 62 per cent during the same period. one hour of surgical procedure further elevated these parameters. our data indicate that isoflurane anaesth ... | 1975 | 1139388 |
| rate of thyroxine secretion by male and laying japanese quail: identification of the radioactive thyroxine degradation component of the multiphasic 131-i curve. | counting of radioactivity in japanese quail in vivo showed a rapid loss of 131-i from the body 12-24 h after the i.v. injection of [131-i]thyroxine (t4), followed by a period of slow decrease in counting rates to 96 h. from comparison of these [131-i]t4 curves with curves for 131-iodide-injected birds and from counts on serum and other tissues in vitro it was concluded that, for japanese quail, the t4 secretion rate should be calculated using serum samples taken during the first 12 h. using this ... | 1975 | 1141813 |
| the structure of bacteriophage t4-specific messenger rnas. i. tightly folded conformation revealed by sedimentation in denaturing solvents. | 1975 | 1142439 | |
| [thyroidal autoregulation in nontoxic goiter patients: normalization of the t3/t4 ratio in serum by treatment with potassium iodide (author's transl)]. | in iodine deficiency areas, an increased t3/t4 ratio in serum was reported earlier. in this study the influence of potassium iodide on the t3/t4 ratio was investigated. treatment with 200 mug ki per day for only 4 weeks normalized the initially elevated t3/t4 ratio in 16 nontoxic goiter patients (24.6 plus or minus 9.9 times 10-3, mean plus or minus s.d., before, respectively 18.9 plus or minus 5.5 times 10-3 after ki treatment, p smaller than 0.0025). the mean basal tsh levels (1.42 plus or min ... | 1975 | 1142708 |
| normal functions of the thyroid gland of the pygmy goat. | normal values were obtained for blood serum pbi, t3 index. t4, and cholesterol in a colony of pygmy goats (n equal to 55) of mixed sex and age. serum pbi values averaged 8.1 plus or minus 1.2 mug/dl with no significant sex differences. the mean t3 index and t4 value were 1.1 plus or minus 0.1 and 7.2 plus or minus 1.1 mug/dl, respectively, with no sex differences. the mean serum cholesterol value was 90.0 mg/dl, with sex differences apparent. serum cholesterol averaged 84.9 mg/dl (n equal to 44) ... | 1975 | 1142725 |
| thyroid activity of spontaneous hypertensive rats. | thyroid activity of both male and female spontaneous hypertensive (sh) rats was studied by measurements of uptake and rate of release of 131-i, urinary excretion of 131-i, and thyroxine secretion rate (tsr). in addition, thyroid glands were removed at death and weighed. radioactivity of the thyroid gland of male rats measured at intervals after administration of 131-i revealed a significantly reduced maximal uptake at 21.5 hr after injection and a reduced rate of release. the mean biological hal ... | 1975 | 1144416 |
| an elevation of plasma ish concentration in spontaneously hypertensive rats (shr). | thyroid activity was tested in two substrains of shr. plasma level and pituitary content of tsh increased significantly in both substrains of shr. as a result, the thyroid weight and thyroidal radioiodine uptake increased significantly. plasma t3 concentration was decreased in kyoto substrain but was normal in nn substrain, while plasma t4 concentration decreased significantly in both substrains. since the pituitary content and plasma level of tsh were significantly higher in spite of the normal ... | 1975 | 1144457 |
| the effect of thyroxine administration on intestinal calcium absorption and calcium binding protein activity in the chick. | thyroxine (t4) injected subcutaneously into 3-week-old chicks (30 mug/kg body weight daily for 8 days) reduced the absorption of calcium from in vivo ligated loops of duodenum and the duodenal calcium binding protein (cabp) activity measured by the chelex ion exchange method. chicks fed either a commercial diet or an artificial diet containing 0.5% or 1.0% calcium showed similar response to treatment with t4. a significant effect of t4 on either calcium absorption of cabp activity could not be d ... | 1975 | 1148887 |
| serum triiodothyronine in solitary autonomous nodules of the thyroid. | seventeen patients with solitary autonomous nodules of the thyroid were studied. eight had clinical features of hyperthyroidism though in some the clinical manifestations were mild. the serum t3 concentration was elevated in all patients with hyperthyroidism but the serum t4 and free thyroxine index were within the normal range in five. all patients with hyperthyroidism subsequently responded to treatment. these findings suggest a high incidence of preferential t3 hypersecretion in hyperthyoidis ... | 1975 | 1149304 |
| diversion of peripheral thyroxine metabolism from activating to inactivating pathways during complete fasting. | in 9 euthyroid obese volunteers, as previously reported, 4 weeks of total caloric deprivation resulted in a striking decrease in serum 3,5;3'-triiodothyronine (t3) concentration. the present studies reveal that this decrease in serum t3 is accompanied by a proportionately similar increase in the serum concentration of 3,3',5' -t3 (reverse t3; rt3). in four additional obese volunteers given suppressive doses of sodium-lthyroxine (t4) for 1 month prior to fasting, serum t3 concentration declined s ... | 1975 | 1150863 |
| measurement of triiodothyronine in unextracted urine. | a radioimmunoassay procedure to measure triiodothyronine (t3) in unextracted urine is described, and the results are compared with those obtained with different assays by others. it is assumed that the radioimmunoassayable t3 in urine represents free t3. euthyroid subjects excreted 1.0 +/- 0.3 mug (mean +/- s.d.) in 24 hours. similar values were obtained in pregnant women, sick patients without thyroid disease, and euthyroid patients receiving antithyroid agents. values were about 3 times normal ... | 1975 | 1151171 |
| assembly of the tail of bacteriophage t4. | the protein products of at least 21 phage genes are needed for the formation of the tail of bacteriophage t4. cells infected with amber mutants defective in these genes are blocked in the assembly process. by characterizing the intermediate structures and unassembled proteins accumulating in mutant-infected cells, we have been able to delineate most of the gene-controlled steps in tail assembly. both the organized structures and unassembled proteins serve as precursors for in vitro tail assembly ... | 1975 | 1152465 |
| 9-aminoacridine mutagenesis of bacteriophage t4 intracellular dna. | most of the intracellular t4 dna made in the presence of 9-aminoacridine is of lower molecular weight than mature t4 dna and does not get packaged into phage particles. using a t4 dna transformation assay, we have examined this intracellular t4 dna for its content of 9-aminoacridine-induced revertants of certain rii gene frameshift mutations. the proportion of acridine-induced revertants in the intracellular dna population is close to that found in the phage progency made in the presence of 9- ... | 1975 | 1152842 |
| enhanced thyroxine metabolism following cutaneous application of microscope immersion oil. | microscope immersion oil, applied to the skin of rats or injected intraperitoneally, led to a substantial increase in the biliary excretion of intravenously injected 125-i-thyroxine (t4), elevated the bile: plasma 125-i ratio, enhanced the biliary clearance rate of plasma 125-i-t-4 and increased the proportion of biliary 125-i present as t-4-glucuronide. immersion oil applied to the skin was at least as effective as injected intraperitoneally. skin application also elevated bile flow, liver weig ... | 1975 | 1153848 |
| changes in plasma thyroxine, triiodothyronine, and tsh during adaptation to iodine deficiency in the rat. | we have measured plasma thyroxine (t4), triiodothyronine (t3), and tsh with specific radioimmunoassays in rats during adaptation to severe iodine deficiency after they had previously received regimens supplying various quantities of iodine. rats were maintained on a high-iodine diet (hid) containing 3 mg iodine/kg or a low-iodine diet (lid) containing 30 mug iodine/kg supplemented with 0.1, 0.2, or 0.4 mug iodine/ml of drinking water before swtiching to kid alone. frequent serial blood samples w ... | 1975 | 1157754 |
| defective thyroglobulin synthesis in an experimental rat thyroid tumor: iodination and thyroid hormone synthesis in isolated tumor thyroglobulin. | purified rat tumor thyroglobulin from the experimental rat thyroid tumor, line 1-1c2, was studied for its thyroid hormone content after in vivo and in vitro iodination and compared with normal and desialylated normal rat thyroglobulin. tumor thyroglobulin had a very low sialic acid and iodine content; after in vivo iodination it contained only small amounts of triiodothyronine (t3) and no detectable thyroxine (t4). after in vitro iodination with 125i it showed a distribution of t3 and t4 very si ... | 1975 | 1157755 |
| increased metabolism of iodothyronines in the rat after short-term cold adaptation. | the effect of 2 weeks of continuous exposure to 4c on the metabolism of iodothyronines in the rat was studied. metabolic clearances of [131i]triiodothyronine ([131]t3) and [125i[-thyroxine ([125i]t4) were increased in exposed animals. estimated absolute turnover of t4 was increased approximately 2-fold as a result of cold exposure. urinary and fecal clearances of labeled hormones were elevated. intracellular total radioiodine and iodothyronine radioiodine concentrations were reduced in liver and ... | 1975 | 1157759 |
| the significance of partial suppressibility of serum thyroxine by triidothyronine administration in euthyroid man. | recent evidence indicates that triiodothyronine (t3) administration may not completely inhibit normal thyroid secretion. to further corroborate this observation, measurement of serum t4-ria concentrations was performed on 15 normal controls (10 men, 5 women; ages 20-42) who were placed on 100 mug of t3 daily for a 5-week period. decrements of 53%, 36%, and 28% from the baseline t4-ria were noted at weeks 1, 2, and 3 respectively. at 3 weeks a nadir t4-ria of 2.5 mug/100 ml was reached which did ... | 1975 | 1159040 |
| determination of common parameters fo iodothyronine metabolism and distribution in man by noncompartmental analysis. | a noncompartmental method is proposed for the calculation of the total distribution volume of exchangeable iodothyronine, the fractional irreversible removal rate from the exchangeable pool, and the metabolic clearance rate of labeled triiodothyronine (t3) and thyroxine (t4). the method depends on a graphic analysis and does not require the use of computers. the results of noncompartmental analysis of t3 and t4 distribution and metabolism are compared to single compartmental calculations in 12 s ... | 1975 | 1159046 |
| thyrotropin-induced hyperthyroidism caused by selective pituitary resistance to thyroid hormone. a new syndrome of "inappropriate secretion of tsh". | an 18-yr-old woman with clinical and laboratory features of hyperthyroidism had persistently elevated serum levels of immunoreative thyrotropin (tsh). during 11 yr of follow-up there had been no evidence of a pituitary tumor. after thyrotropin-releasing hormone (trh), there was a marked increase in tsh and secondarily in triiodothyronine (t3), the latter observation confirming the biologic activity of the tsh. exogenous t3 raised serum t3 and several measurements of peripheral thyroid hormone ef ... | 1975 | 1159077 |
| reduced peripheral conversion of thyroxine to triiodothyronine in patients with hepatic cirrhosis. | the role of liver in the peripheral conversion of thyroxine (t4) to triiodothyronine (t3) was studied in normal subjects and patients with alcoholic liver disease by measurement of thyrotrophin (tsh) and total and free t4 and t3 in randomand serial serum samples. also, t4 to t3 conversion rates and t3 disposal rates were compared by noncompartmental analysis. while the mean total serum t4 values were similar for the two groups, 8.6 and 8.1 mug/kl, the mean free t4 value was significantly higher ... | 1975 | 1159078 |
| molecular weight of dna from actinophage msp2. | actinophage msp2 is infectious for streptomyces venezuelae s13. based upon electron microscopy of coliphage t4 mixed with msp2, msp2 had a head about 48 +/- 2 nm wide and 87 +/- 5 nm long. dna from polyoma virus and from coliphages t4 and t7 served as reference markers in estimating the molecular weight of msp2 dna from sedimentation in sucrose gradients. denatured msp2 dna was estimated to be about 17 x 10(6) and double-stranded msp2 dna was about (36 +/- 1.6) x 10(6) in molecular weight. | 1975 | 1159900 |
| thyroxine (t4) immunoassay using a filter paper blood samples for screening of neonates for hypothyroidism. | a rapid, sensitive radioimmunoassay for thyroxine (t4) is described which requires a specimen of dried blood on filter paper. one milliliter of glycine-acetate buffer containing anti-t4 antibody, tracer t4, and sodium salicylate is added to a tube containing a 1/8-inch dot of the filter paper specimen. after incubation overnight, bound and free hormone are separated by addition of dextran-coated charcoal. quantitation is obtained using a standard curve prepared from dots of dried blood sample ... | 1975 | 1161349 |
| [radioimmunoanalysis for t3 and t4 in normal subjects and in patients with thyroid pathology (author's transl)]. | 1975 | 1162190 | |
| thyroid hormone-induced changes in body temperature and metabolism during exercise in dogs. | changes in serum total thyroxine concentration (tt4) and the effects of thyroxine (t4) and 3,5,3'-triiodothyronine (t3) injection on plasma free fatty acid (ffa) level and rectal temperature (tre) responses were measured in six dogs at rest and during 1 h of submaximal treadmill exercise. at rest there were no increases in ffa level or tre up to 72 h after thyroid hormone treatment. during exercise, 5 h after a single t4 injection (0.1 mg/kg), there was a) a significant increase in tt4, although ... | 1975 | 1163655 |
| the molecular size and conformation of the chloroplast dna from higher plants. | covalently closed circular choloroplast dna (ctdna) molecules have been isolated from pea, bean, spinach, lettuce, corn and oat plants by ethidium bromide/cesium choloride density-gradient entrifugation. as much as 30-40% of the total ctdna could be isolated as closed circular dna molecules and up to 80% of the total ctdna was found in the form of circular molecules. the size of pea, spinach, lettuce, corn and oat ctdna relative to an internal standard (phix174 replicative form ii monomer dna) w ... | 1975 | 1164522 |
| analysis of expression of the rii gene function of bacteriophage t4. | temperature-sensitive (ts) mutants of the t4 phage rii gene were islated and used in temperature shift experiments that revelaed two different expressions for the normal rii (rii+) gene function in vivo: (i) an early expression (0 to 12 min postinfection at 30 c) that prevents restriction of t4 growth in escherichia coli hosts lysogenic for gamma phage, and (ii) a later expression (12 to 18 min postinfection at 30 c) that results in restriction of t4 growth when the phage dna ligase (gene 30) is ... | 1975 | 1165600 |
| serum tri-iodothyronine, thyroxine, and thyrotrophin concentrations in newborns during the first 2 days of life. | the serum concentrations of tri-iodothyronine (t3), thyroxine (t4), and thyrotrophin (tsh) were measured in 10 term newborn infants between birth and the age of 2 days by radioimmunoassay. the mean concentration of t3 in maternal serum was 1.62 mug/l, and it increased from the low cord blood level of 0-63 mug/l to the peak value of 1-76 mug/l within the first 2 hours of life. mean serum t4 concentrations increased from the cord blood level of 145 mug/l to the peak value of 205 mug/l within the f ... | 1975 | 1167070 |
| raised serum tsh in hypothyroidism. | it is desirable to detect early hypothyroidism of the mildest degree even before conventional tests of thyroid function become abnormal. serum tsh levels (normal: undetectable to 4 muu/ml) rise in patients with mild hypothyroidism long before serum t4 and t3 levels fall. in the patient described the serum tsh level was 310 muu/ml, while other tests of thyroid function gave normal results. after treatment with thyroxine, serum tsh returned to normal. it should now be accepted that patients with m ... | 1975 | 1167071 |
| thyroid function during exchange transfusion. | the changes in plasma thyroid hormone concentration were studied during exchange transfusion performed for haemolytic disease. 24 transfusions were performed using blood preserved with acid-citrate and dextrose and in 11 cases 10 or 50 mug glucagon was added to the donor blood. plasma tri-iodothyronine (t3), thyroxine (t4), thyrotropin (tsh), thyroid hormone binding capacity, and free thyroxine index were measured in the donor blood and in the infant at the start and at intervals during the tran ... | 1975 | 1168447 |
| twenty-four-hour prolactin profiles in normal and disease states: failure of thyroxine to modify prolactin secretion. | in order to assess the role of thyroid hormone on physiologically and pharmacologically induced prolactin (prl) secretion, serum prl concentrations were measured in 4 normal women and 4 women with various endocrinopathies before, and 4 to 6 days following, the ingestion of l-thyroxine (t4). a single 1.5 to 3.0 mg dose of oral t4 produced approximately a 2-fold increase in serum t4. exogenous t4 did not significantly alter the mean concentration, or the pattern of prl secretion during a 24-h inte ... | 1975 | 1168655 |
| the binding of thyroid hormones to bovine serum albumin as measured by circular dichroism. | the circular dichroism (cd) spectra of 3,5,3'-triiodothyronine-bovine serum albumin (t3-bsa) and thyroxine-bovine serum albumin (t4-bsa) complexes were measured at the various ratios of t3 or t4 to bsa (t/p) in the wavelength region of 200-350 nm. no spectral change was observed in the chromophoric wavelength region of 250-350 nm. the value of [omicron] at 319 nm of the induced cotton effect increased with increase of the t/p ratio in both complexes, and in t4-bsa complex, it increased remarkabl ... | 1975 | 1169236 |
| neutral amino acid transport pathways in uptake of l-thyroxine by ehrlich ascites cells. | neutral amino acid pathways were investigated as possible mediators of l-thyroxine (t4) entry into ascites cells. 14c-labeled t4, alpha-aminoisobutyric acid (aib), l-phenylalanine (phe), and cycloleucine (cleu) uptakes were measured at initial extracellular amino acid concentrations of 1 mum in a modified krebs-ringer phosphate buffer, ph 7.4. t4 uptake was markedly slower than that of aib, phe, or cleu over a 25-min period. t4 uptake was neither competitively stimulated nor inhibited by either ... | 1975 | 1170764 |
| t4 dna polymerase has a lower apparent km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template. | 1975 | 1170851 | |
| [effect of mmi on the serum t3 and t4 levels in hyperthyroid patients (author's transl)]. | effect of 1-methyl-2-mercaptoimidazole (mmi) on the serum triiodothyronine (t3) and thyroxine (t4) was studied in six patients with hyperthyroidism. from 30 to 60 mg of mmi per day were administered to the patients orally. serum t3 was measured by radioimmunoassay and t4 by radioimmunoassay of tetrasorb kit (abbott lab.). serum t4/t3 ratios in these patients were low initially, but increased to normal range in two cases immediately after the administration of mmi. but they were unchanged at firs ... | 1975 | 1171030 |
| [changes in blood constituents induced by noradrenaline infusion and acute cold exposure in rats treated chronically with noradrenaline and thyroxine (author's transl)]. | rats were treated with noradrenaline (na), thyroxine (t4), na plus t4, and na plus acth for 28 days, and changes in rectal temperature and blood concentrations of nonesterified fatty acids (nefa), sugar, and lactate were observed following na infusion and acute cold exposure. results obtained were compared with those found in cold-acclimated rats. na infusion caused a marked elevation of the rectal temperature in cold-acclimated rats and na plus t4-treated ones. no significant changes were obser ... | 1975 | 1171056 |
| inhibition by somatostatin of basal levels of serum thyrotropin (tsh) in normal men. | to elucidate the effect of somatostatin on basal, unstimulated tsh secretion 6 young healthy nonobese men were studied with blood samples every 15 min from 2300 h to 0500 h. somatostatin 1 mg was indused over a 2-h period from 0100 h. one of the subjects was also studied with half hourly blood samples throughout a 26-h period without receiving somatostatin. in all cases the high night levels of serum thyrotropin (tsh) were significantly suppressed by somatostatin. the fall of tsh continued throu ... | 1975 | 1171113 |
| [simultaneous measurements of free thyroxine and triiodothyronine fractions in human serum by means of equilibrium dialysis (author's transl)]. | simultaneous measurements of serum free thyroxine (t4) and triiodothyronine (t3) fractions were studied using a modification of equilibrium dialysis described by sterling and brenner. to 1.2 ml of the serum to be assayed, 131i-t4 and 125i-t3 were added in a concentration of 2 mug/dl and 25 ng/dl, respectively, both of which were preliminarily dialysed according to schussler and plager. half ml of the serum with tracers added was dialysed against 9 ml of phosphate buffer (ionic strength 0.15, ph ... | 1975 | 1171788 |
| [stimulatory effect of prostaglandins on biosynthesis of thyroid hormones and plasma tsh levels in the rats (author's transl)]. | it is now postulated that prostaglandins (pg) are widely distributed in mammalian tissues and stimulate adenyl cyclase-cyclic amp system in various organs. the present study was undertaken in an attempt to clarify the effect of pg on pituitary-thyroid axis in vivo. rats were maintained on a low-iodine diet for 8 days. the animals received graded doses (10-500 mug) of pge1, pge2 and pgf2alpha ip daily from day 5 to the day of autopsy. the 4-hr thyroid 131o uptake was consistenly higher in 10 mug ... | 1975 | 1171791 |
| intragenic complementation by gene 42 amber mutations of bacteriophage t4. | phage t4 amber mutants defective in gene 42 (dcmp hydroxymethylase) were shown by in vivo and in vitro experiments to participate in both positive and negative intragenic complementation. this argues that incomplete polypeptide chains can participate in subunit interaction. | 1975 | 1172535 |
| effect of the exposure to cold on the extrathyroidal conversion of l-thyroxine to triiodo-l-thyronine, and on intramitochondrial alpha-glycerophosphate dehydrogenase activity in thyroidectomized rats on l-thyroxine. | thyroidectomized rats have been injected daily with 125-i labelled l-thyroxine (t4) and, once isotopic equilibrium was attained, divided into cold-exposed (4-10 degrees c) and control (21-24 degrees c) groups, the daily t4 administration being continued till the end of the experiment. fourteen days after onset of cold exposure, the total i of different organs and of the carcass was determined and the tissues submitted to extraction and paper chromatography for the separation of t4 and t4-derived ... | 1975 | 1173014 |
| reduction in extrathyroidal triiodothyronine production by propylthiouracil in man. | to determine if propylthiouracil (ptu) inhibited extrathyroidal thyroxine (t4) to triiodothyronine (t3) conversion in man, ptu was administered to t4-treated hypothyroid patients and serial measurements of t4, t3, and thyrotropin (tsh) carried out. all patients had proven thyroidal hypothyroidism and had been receiving 0.1 or 0.2 mg t4 daily for at least 2 mo before study. hormone measurements were made for 5 consecutive days before and daily during a 7-day treatment period with ptu, 1,000 mg/da ... | 1975 | 1173242 |
| age dependent changes in iodine metabolism and thyroid function. | absolute iodine uptake (aiu), serum thyroxine (t4), free t4 index and serum triiodothyronine (t3) were studied in 48 women and 63 men aged 20 to 90 years. aiu decreased with age in both sexes to values in the oldest age groups of about 60% of the values in the youngest age groups. aiu values for females were about 75% of the values for males. free t4 index was found to be unaltered with increasing age but serum t3 decreased with age. these findings and the previous demonstrated decrease in t4 de ... | 1975 | 1173311 |
| role of growth hormone in the enhancement of the propylthiouracil-induced goitrogenesis by small doses of thyroxine. | the potentiation of the propylthiouracil (ptu)-induced goitrogenesis after chronic administration of small doses of thyroid hormone has been attributed to the high circulating level of thyrotrophin (tsh) or to the re-instatement of insulin. in re-examining this problem radioimmunoassayable concentrations of tsh, thyroxine (t4), insulin, and growth hormone (gh) were observed in sera of rats at sequential intervals after surgical or chemical thyroidectomy and after thyroidectomy and replacement th ... | 1975 | 1173483 |
| receptors mediating epinephrine effect on thyroid 131-i uptake and thyroxine synthesis in the rat. | the adrenergic receptors mediating the inhibitory effect of epinephrine on the rat thyroid in vivo have been studied by the use of more specific alpha- and beta-adrenergic stimulators, and blocking agents. the effect of methoxamine was similar to that of epinephrine, while isoproterenol stimulated 131-i uptake slightly, and did not alter the ratio of monoiodotyrosine (mit) to diiodotyrosine (dit). however, like methoxamine, isoproterenol resulted in a decrease in thyroxine (t4) formation. phento ... | 1975 | 1173485 |
| serum tsh and serum t3 levels during normal menstrual cycles and during cycles on oral contraceptives. | serum tsh and serum t3 levels were studied in 35 normally menstruating women and in 35 women on oral contraceptives in order to find out whether the levels of tsh and t3 were related to the menstrual cycle or changed by oral contraceptives. serum tsh and t3 were found to be unchanged throughout the normal menstrual cycles and during the cycles of oral contraceptives. the tsh level was higher in the women on oral contraceptives than in the normally menstruating women. this might be due to a direc ... | 1975 | 1173500 |
| thyroxine metabolism in the rat: effect of varying doses of exogenous thyroxine. | experiments were performed to determine quantitatively the peripheral metabolism of exogenous thyroxine (t4) in rats brought to isotopic equilibrium with doses of -131i-t4 ranging from 1 to 20 mug/100 g body weight/day. it was found that, although the absolute amount of t4 either deiodinated and excreted as iodide in urine or excreted as t4 in faeces increased as the dose of t4 increased, the percentage of hormone excreted by either pathway at each dose level was relatively constant. in other ... | 1975 | 1173961 |
| acute effects of corticosteroids on thyroid activity in graves' disease. | we studied the effects of administration of dexamethasone, 2 mg orally every 6 hr for 4 doses, on circulating thyroid hormone levels in hyperthyroid graves' disease patients and in normal subjects. serum triiodothyronine (t3), thyroxine (t4) and thyroglobulin (tg) fell significantly below baseline values within 24 to 48 h after the first dose of dexamethasone in hyperthyroid patients; the values returned to or toward baseline levels in the subsequent 5 to 6 days. serum t3 fell transiently in nor ... | 1975 | 1174132 |
| iodoamino acid content and distribution in normal and abnormal human thyroids. | iodoamino acid content and distribution were measured in adenomas, nodular goiters, thyroiditis and carcinomas and compared to thyroids from patients who died suddenly and normal thyroid tissues originally adjacent to diseased tissue. tissues were hydrolyzed with pronase, derivatized and analyzed by gas chromatography as had previously been reported for rat thyroids. considerable overlap in values was found among adenomas, nodular goiters and papillary and follicular carcinomas as compared to va ... | 1975 | 1174133 |
| effects of hyper- and hypothyroidism on serum lh and fsh levels in intact and gonadectomized male and female rats. | the effects of hypo- and hyper-thyroidism on serum lh and fsh were determined in both intact and castrated male and female rats. thyro-parathyroidectomy (tx) for 30 days in rats with intact gonads resulted in a significant reduction in serum lh and fsh, and also in a decrease in serum testosterone in males. administration of 2.5 mug thyroxine (t4)/100 g bw to tx rats of both sexes returned serum lh and fsh levels to those of intact rats, and in males also restored normal serum testosterone level ... | 1975 | 1175507 |
| serum t3 level in the patients with hyperthyroidism after therapy. | serum t3 level in various thyroid diseases was determined in unextracted serum with the dainabot kit for t3 ria. the serum t3 level in 33 normal subjects was 0.8-1.6 ng/ml. it was 5.7 +/- 3.5 ng/ml (mean +/- s.d.) in 36 hyperthyroid patients, and undetectable to 0.8 ng/ml in 21 hypothyroid patients. generally the serum t4 and serum t3 decreased in parallel after radioiodine therapy for hyperthyroidism. however, in some cases the serum t3 level remained high in spite of normalized serum t4 after ... | 1975 | 1175526 |
| [diurnal rhythms of thyroid hormones in rooster chicks (gallus domesticus). 3. studies on the changes in deiodination rate of thyroxine-i-131]. | as a measure for the availability and efficiency of the so-called free hormone to repress the thyreotropin-secretion (mouriz et al., endocr. 79, 248-260 (1966) we investigated the deiodination of thyroxine-i-131 (t4-i-131). this was done with special regard to a possible cyclic variation. by means of the reutilisation we could measure the removed radio-iodide in the thyroid 1-1/2 hours after t4-i-131 application. using i-125 as a reference-isotope for the radioiodine uptake we could specify the ... | 1975 | 1175533 |
| transcription of dnas of known sequence after injection into eggs and oocytes of xenopus laevis. | 1. when the synthetic polynucleotide, poly[d(a-t) - d(a-t)] is injected into the eggs and oocytes of xenopus laevis, a stimulation of rna synthesis results. analysis of extracted rna by high-voltage electrophoresis, shows that this stimulation of rna synthesis is due to the production of poly[r(a-u)] transcripts. the rate of poly[r(a-u)] synthesis is calculated to be at least ten-fold greater in eggs than in oocytes. the amount of poly[r(a-u)] detectable in injected eggs has reached a maximum by ... | 1975 | 1175643 |
| circadian variations in concentrations of plasma thyroxine and triiodothyronine in man. | the plasma concentrations of thyroxine (t4), triiodothyronine (t3), and total protein (pr) were measured at 2-h intervals in 8 male subjects during two 24-h periods. plasma t4 and t3 levels varied significantly during the day. t4 values were highest at 0900 hours and thereafter declined rapidly reaching lowest levels at 1500-1700 hours (mean decrement, 13.2% of 0.00-hour value). plasma t3 was highest at 0900 hours and lowest at 1700-1900 hours (mean decrement, 16.7% of 0900-hour value). fluctuat ... | 1975 | 1176392 |
| the evaluation of treatment of patients with extensive squamous cancer of the vocal cords. | fifty-one patients with previously untreated squamous carcinoma originating on the true vocal cord, staged t4, were analyzed. forty-eight of the 51 patients were selected for treatment. total laryngectomy was the minimal surgical procedure, and the pathologist confirmed the presence of cancer outside the confines of the larynx in 47 of 48 patients. seventy-three percent of the patients were thought clinically to have negative nodes, but examination by the pathologist showed a 20 percent false ne ... | 1975 | 1177634 |
| laryngeal carcinomas. | this study is based upon 214 cases of laryngeal carcinoma treated at the foundation curie, paris, france, from january, 1958, to 1968. the results in terms of five-year survival have been: (see article). a statistical study of these results was made by professor daudel and demonstrated the absence of significant difference for the groups of tumors classed t1 and in stage i and ii treated either by radiocobalt or by surgery, whether followed or not by radiotherapy. by contrast there was a clearly ... | 1975 | 1177638 |
| rna polymerase binding sites isolated from t4dna: analysis of oligopyrimidine sequences constituting preinitiation and initiation complexes. | oligopyrimidines which contain 5'-hydroxymethylcytosine instead of cytosine were separated by thin layer chromatography. using this method, the oligopyrimidine pattern of rna polymerase binding sites, isolated from t4dna, was evaluated quantitatively. the analysis shows that 1. the rna polymerase binding sites on t4 dna obtained under low salt conditions in absence of triphosphates, are a-t-rich as compared with total t4 dna. 2. the a-t base pairs stand mainly in alternating position. on the ave ... | 1975 | 1177865 |
| thyroid metabolism in the recessive sex-linked dwarf female chicken. 1. age related changes in thyroid hormone synthesis and circulating thyroid hormone levels. | age related changes in the levels of circulating thyroid hormones as well as the type of hormones synthesized in the thyroid glands from normal and sex-linked recessive dwarf, female chickens were studied. the impact of the presence of the dwarf gene on the parameters measured was minimal but significant alterations in the types of hormones produced in the thyroid gland with increasing age were observed. as the birds approached sexual maturity, the synthesis of triiodothyronine increased sharply ... | 1975 | 1178606 |
| thyroid metabolism in the recessive sex-linked dwarf female chicken. 3. the influence of exogenous thyroid hormones in glycogen metabolism. | the influence of exogenous thyroid hormones on glycogen-body and liver glycogen was studied in fasted and full-fed 3 wk. old dwarf and non-dwarf white leghorn chickens. the results indicated that in contrast to normals, the glycogen-body of the dwarf was still physiologically active at age 3 wks. and they also exhibited a relatively higher liver glycogen concentration. the liver glycogen concentration was significantly higher in t3-treated, full-fed dwarf chickens when compared with other groups ... | 1975 | 1178607 |
| thyroid metabolism in the recessive sex-linked dwarf female chicken. 4. the influence of exogenous thyroid hormones on amino acid uptake by plasma and tissues. | the influence of exogenous triiodothyronine (t3) or tetraiodothyronine (t4) on the incorporation of 3h-labelled methionine, alanine and lysine into plasma, liver and kidney was studied in 4 wk. old dwarf and non-dwarf female, white leghorn chickens. the response to exogenous t3 or t4 of the birds was directly dependent on the dwarf status as well as the tissue and/or amino acid studied. in general, there was a decreased amino acid uptake by dwarfs and t3 and/or t4 depressed amino acid uptake in ... | 1975 | 1178608 |