Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [course of thyroxinemia during the development of the chick embryo. influence of hypophysectomy]. | plasma thyroxine (t4) level of intact and hypophysectomized chick embryos has been determined for the incubation interval 10 to 18 days. t4, which is the main circulating thyroid hormone, exists as early as day 10. the plasma t4 level of controls increases as power function of time from day 10 to day 17. hypophysectomy (on day 2) produces a decrease of the plasma t4 level, but only from day 11, which accounts for the beginning of a pituitary control of the thyroid secretion after day 10. | 1976 | 825240 |
| studies on the control of thyrotrophin secretion in normal man. | the effect of graded doses of thyroxine (t4) and triiodothyronine (t3) separately on the pituitary-thyroid axis has been studied in normal subjects. it has been demonstrated that small doses (50 mug t4 or 20 mug t3) cause significant suppression of the pituitary-thyroid axis. there was a strong negative correlation between the tsh value 20 min after trh and the dose of thyroid hormone. t4 supplements led to a progressive dose-related rise in serum t4 and no significant change in t3 until high d ... | 1976 | 825331 |
| effect of thyrotropin-releasing hormone on secretion of thyrotropin, prolactin, thyroxine, and triiodothyronine in pregnant and fetal rhesus monkeys. | the effect of thyrotropin-releasing hormone (trh) on the pituitary-thyroid axis and on prolactin secretion was studied in pregnant rhesus monkeys during the latter period of gestation and in non-pregnant female controls. the baseline plasma concentrations of tsh, t3, t4, and prolactin (prl) of pregnant monkeys did not differ from those of non-pregnant monkeys. after administration of trh, plasma prolactin rose to higher levels in pregnant monkeys than in non-pregnant monkeys whereas there was a ... | 1976 | 825527 |
| isolated thyrotropin deficiency with thyrotropin-releasing-hormone induced tsh secretion and thyroidal release. | a patient with isolated thyrotropin (tsh) deficiency was studied. prior to treatment with thyroid hormone, administration of thyrotropin releasing hormone (trh) produced no increment in serum tsh and a normal increase in plasma prolactin (prl). in order to explore whether physiologic increases in serum tsh might be occurring below the limits of detectability of tsh by radioimmunoassay, a double isotope technique of assessing thyroidal secretion secondary to release of tsh was employed. the patie ... | 1976 | 825528 |
| trh by slow, continuous infusion: an antidepressant? | a slow, continuous infusion of 1000 mug trh (thyrotropin releasing hormone) over a period of 4 h had a very faint and diffuse short-lasting beneficial effect on a group of 10 depressive patients. this was assessed in a double blind cross-over trial with placebo. the effect was of no therapeutic value. no difference was found between the depressive patients and a control group of normal subjects in tsh response, t3 resin uptake, t4 or free thyroxine index values as a consequence of the trh infusi ... | 1976 | 825895 |
| [the hypophyseal tsh reserve in surgical patients with bland goiter and recurrent goiter]. | serum-t4, -t3, fti, basal tsh and trh-induced tsh response were measured in 80 patients with simple nontoxic goiter and in 24 patients with nontoxic recurrent goiter in a surgical unit. 55 of the 80 patients with simple goiter and 11 patients with recurrent goiter had normal individual tsh values. mean fti and serum t3 were not statistically different from age and sex related normal values, except serum-t3, which was above normal in elderly goiter patients. the incidence of elevated tsh reserve ... | 1976 | 825961 |
| [experimental and clinical studies of t3 uptake and blood t4 concentration measured by tri-tab and tetra-tab kits]. | 1976 | 826359 | |
| tbg deficiency in male euthyroid patient with hypothyroxinemia. | tbg deficiency in euthyroid male patient with contradictory values for tbi and t4 was demonstrated. the deficiency seems to be a genetic anomaly and did not affect the patients health. | 1976 | 826390 |
| propranolol in the treatment of thyrotoxicosis by subtotal thyroidectomy. | subtotal thyroidectomy was performed in 40 patients with thyrotoxicosis in whom propranolol alone was used as preparation for surgery. propranolol was given orally in a dose of 40 mg every 6 h for a mean preoperative period of 17 days (range 4-60 days) and continued for seven days after operation. the mean +/- se blood loss at operation was only 160 +/- 20 ml. the period of follow-up was from three to nine months. recurrent thyrotoxicosis has not occurred in any patient. low levels of total seru ... | 1976 | 826544 |
| measurement of 3,3',5'-triiodothyroinine (reverse t3), 3,3'-l-diiodothyronine, t3 and t4 in human amniotic fluid and in cord and maternal serum. | in order to assess fetal function at term, we have investigated parameters of thyroid hormone secretion and degradation in human amniotic fluid and in cord and maternal sera at delivery. the parameters measured included 3,3' l-diiodothyronine (3,3't2), 3,3',5'-triiodothyronine (reverse t3), 3,3',5'-triiodothyronine (t3), thyroxine (t4), dialyzable t3 and t4, thyroxine binding globulin (tbg),and total iodine. the mean (+/- se) 3,3't2 concentrations in cord sera, amniotic fluid, and maternal sera ... | 1976 | 826545 |
| inappropriate tsh secretion. paradoxical rise in serum tsh in response to thyroxine therapy. | serum thytropin (tsh) in a 45-year-old woman suffering from hashimoto's thyroiditis and hypothyroidism rose over a period of 8 months from 23 to 98 muu/ml while on 0.15 mg of thyroxine daily. a significant pituitary tumor was excluded and the response of other anterior pituitary hormones to provocative stimuli were normal. the tsh response to thyrotropin releasing hormone (trh) carried out after a year of therapy and while on 0.15 mg t4 was exaggerated and required 100 mug of triiodothyronine (t ... | 1976 | 826546 |
| [the influence on the thyroid hormone system by long-term anticonvulsive therapy with valproat (author's transl)]. | valproat lowers the serum concentraiton of pbi and t4 in epileptic children. since this reduction is not so pronounced with pbi as with t4, the difference in comparison with the controls could not be statistically verified with the desired probability of error. the reduction of general thyroxine can be statistically verified when compared with healthy control individuals. free thyroxine is not influenced by valproat therapy. the tbg tsh and the t3 in vitro test do not indicate variations from th ... | 1976 | 826735 |
| effect of a large dose of thyrotrophin releasing factor on pituitary and thyroid function in patients with parkinsonism. | thyrotrophin releasing factor (trf) was given intravenously in doses of 0-5 mg and 20 mg to six patients with parkinsonism treated with l-dopa. plasma thyrotrophin (tsh), prolactin, lutenizing hormone (lh), follicle stimulating hormone (fsh), thyroxine (t4) and triiodothyronine (t3) were measured before and after trf infusion. the observation that fsh and lh did not change in response to either dose of trf confirmed specificity of the effects trf for certain anterior pituitary functions. the pla ... | 1976 | 827396 |
| augmentation of thyrotropin responses to thyrotropin-releasing hormone following inorganic iodide. | eight euthryoid subjects were administered 500 mug of thyrotropin-releasing hormone intravenously before and after the administration of 10 mg potassium iodide daily for 7 days. basal serum concentrations of tsh and the response of tsh to trh were significantly increased following iodide treatment, while serum levels of t4 and t3 did not differ significantly. it is suggested that the administration of 10 mg/day of potassium iodide can produce similar changes in serum tsh to those in previous rep ... | 1976 | 827433 |
| serum levels of thyrotropin, prolactin, growth hormone, triiodothyronine and thyroxine after oral administration of thyrotropin releasing hormone in hypothyroid and hyperthyroid patients. | after peroral administration of trh there were found elevated responses of tsh and hpr in hypothyroidism but not in hyperthyroidism. while the basal levels of tsh were elevated in hypothyroidism and depressed in hyperthyroidism, no such difference has been found in the case of basal levels of hpr. after trh stimulation the levels of hgh decreased especially in hyperthyroidism; in patients with acromegaly serum hgh level increased after trh. serum t4 increased only in euthyroid persons after 2 an ... | 1976 | 827434 |
| factors influencing the early plasma disappearance rate and liver uptake of thyroxine. | the early disappearance from plasma (t 1/2) of i.v. administered thyroxine (t4) labelled with 131i was studied in order to find a simple biochemical parameter with which it could be correlated in health and disease. the radioactivity of the liver was also measured by surface counting to determine the time for the peak liver count rate. the t 1/2 for the 131i-t4 showed a correlation of borderline significance with t4 in plasma but a good correlation was found with t4-binding globulin (tbg) in pla ... | 1976 | 827838 |
| serum thyrotropin response to thyrotropin-releasing hormone and free thyroid hormone indices in patients with familiar thyroxine-binding globulin deficiency. | the response in serum thyrotropin (tsh) to synthetic thyrotropin-releasing hormone (trh) as well as serum free thyroxine index (ft4i) and free triiodothyronine index (ft3i) was investigated in six patients with familial thyroxine-binding-globulin (tbg) deficiency. the total serum thyroxine (t4) and triiodothyronine (t3) concentrations were significantly decreased, compared with those of normal subjects (3,4 +/- 0.9 microng/dl, mean +/- s.d. vs. 9.0 +/- 1.5 microng/dl, p less than 0.01 and 87 +/- ... | 1976 | 828576 |
| [the hypothalamic-pituitary thyroid axis: studies on the regulatory role of thyrotropin releasing hormone (trh) (author's transl)]. | these studies were undertaken to clarify the physiological role of thyrotropin releasing hormone (trh) in regulation of the hypothalamic pituitary thyroid axis. synthetic trh was administered both acutely as a single intravenous dose of 500 micrograms (trh iv) and chronically (trh p.o.), in the form of repetitive oral doses (10 mg b.i.d. for 4 days) to 21 normal, 26 hypothyroid, and 14 hyperthyroid subjects. serial determinations were made of the serum levels of thyrotropin (tsh), triiodothyroni ... | 1976 | 828584 |
| significance of urinary excretion of triiodothyronine (t3) and thyroxine (t4). | 1976 | 828618 | |
| measurement of cellular copper levels in bacillus megaterium during exponential growth and sporulation. | both atomic absorption spectrophotometry (aas) and neutron activation analysis have been utilized to determine cellular cu levels in bacillus megaterium atcc 19213. both methods were selected for their sensitivity to detection of nanogram quantities of cu. data from both methods demonstrated identical patterms of cu uptake during exponenetial growth and sporulation. late exponential phase cells contained less cu than postexponential t2 cells while t5 cells contained amounts equivalent to exponen ... | 1976 | 829580 |
| [secretion of tsh and stimulation-ability of the hypophysis after long-term suppression therapy in euthyroid nodular goiter and residual thyroid gland after subtotal resection of goiter]. | in 51 patients, 33 with euthyroid goiter and 18 after subtotal thyroidectomy, plasma concentrations of t4, t3, and tsh before and after stimulation with trf were determined under long term suppression with thyroid hormone and 1,2, and 4 weeks after stopping it. even after complete suppression (delta tsh less than 0, 5 muu/ml) resumption of tsh secretion occurred within 4 weeks in all but two. a significantly higher increase of tsh and somewhat lower thyroid-hormone concentrations turned out in o ... | 1976 | 829603 |
| simultaneous initiation of synthesis of bacteriophage t4 dna and of deoxyribonucleotides. | in earlier reports we have suggested that bacteriophate t4 dna replication occurs in a complex composed of the proteins required for polymerization and the system of enzymes synthesizing the deoxyribonucleoside triphosphate precursors of dna. t4-induced dcmp hydroxymethylase and dtmp synthetase, though demonstrable in extracts soon after infection, are not active in vivo until about 5 min. the in vivo activities increase exponentially for approximately 15 min and then become constant. we have su ... | 1976 | 1062786 |
| [normal levels of total t3 and t4 in the 1st week of life determined by radioimmunoassay]. | 1976 | 1065336 | |
| a gene of bacteriophage t4 whose product prevents true late transcription on cytosine-containing t4 dna. | t-even coliphages have 5-hydroxymethylcytosine in their dna instead of cytosine. in some t4 mutants, the replicated dna contains cytosine, but then no late gene products are made. we show that the inability to make late gene products with cytosine-containing t4 dna is due to a t4 gene products. this gene product, while probably nonessential under normal conditions, interacts with an essential part of the transcription apparatus. mutations in this gene allow viable t4 particles to be made whose d ... | 1976 | 1067605 |
| heat mutagenesis in bacteriophage t4: the transversion pathway. | heat induces transversions (as well as transitions) at g-c base pairs in bacteriophage t4. the target base for transversions is guanine,which is converted to a product which is sometimes replicated and transcribed as a pyrimidine.a model for this process is proposed in which the deoxyguanosine glycosidic bond migrates from n9 to n2: the resulting deoxyneoguanosine may pair with normal guanine to produce g-c leads to c-g transversions. | 1976 | 1069305 |
| protein cleavage during virus assembly: a novel specificity of assembly dependent cleavage in bacteriophage t4. | cleavage of precursor proteins occurs during assembly of numerous viruses. seven bacteriophage t4 head-related proteins areknown to be cleaved during morphogenesis. sequences surrounding the cleavage sites in t4 head precursors p23 and ipiii are reported here. we previously determined the sequences of precursor and processed forms of ipii and ipi. cleavage occurs at a glutamyl-alanyl bond in each protein. by comparison of sequences around five cleaved and four uncleaved glu-ala bonds in head pre ... | 1976 | 1069310 |
| [spino-thalamic cordotomy in cancerous pain. results of a series of 124 patients operated on by the direct posterior approach]. | the authors -- about a series of 124 cancerous patients treated during the 12 last years with open spino-thalamic cordotomy for intractable pain -- have tried to evaluate effectiveness of the operation with regard to its levels in relation to the site of pain. patients suffering median or bilateral perineo-pelvic pain, isolated or associated with algias in one or both legs (group i: 50%) underwent a bilateral c8-c6 cordotomy in one stage. patients with the same perineo-pelvic cancers but sufferi ... | 1976 | 1071136 |
| effects of hyperthyroidism and hypothyroidism on rat growth hormone release induced by thyrotropin-releasing hormone. | the effect of synthetic thyrotropin-releasing hormone (trh) on the release of growth hormone (gh) and thyroid-stimulating hormone (tsh) was investigated in euthyroid, hypothyroid, and hyperthyroid rats under urethane anesthesia. in euthyroid control rats, intravenous injection of trh (200 ng/100 g bw) resulted in a significant increase in both plasma gh and tsh. in rats made hypothyroid by treatment with propylthiouracil or by thyroidectomy, basal gh and tsh levels were significantly elevated wi ... | 1976 | 819251 |
| on the time course of thyrotropin suppression by high doses of thyroid hormones. | basal and stimulated tsh decreased progressively. basal tsh was suppressed below the detection limit of 0.4 muu/ml after 74 h in 2 of the t3 and all of the t4 treated individuals. at this time in both groups 3 individuals could be significantly stimulated by trh (abour 5% of the pretreatment stimulation). there was no significant difference in the time course of suppression obtained by t3 or t4 through plasma t3 levels in the t4 treated group were considerably lower. | 1976 | 819291 |
| [measurement of thyroxine binding globulin by competitive ligand binding assay (clba) (author's transl)]. | the competitive ligand binding assay (clba) first described by chopra et al. ((1972) j. clin. endocrinol. metab. 35, 565-573) is a convenient routine method for the accurate measurement of thyroxine binding globulin in large numbers of serum samples. the assay is based on the partition of a constant quantity of radiolabelled t3 between a fixed quantity of rabbit t3 antibodies and the thyroxine binding globulin of the serum, after prior removal of t3 and t4 from the serum with an anion exchange r ... | 1976 | 819614 |
| [restitution of the thyrotropic function in goiter patients after discontinuing a long-term suppression therapy with thyroid hormones]. | 27 subjects with a negative trh test were selected among patients with euthyroid goiter treated by thyroid hormones. the hormone therapy was stopped and t3 and t4 concentrations, basal and trh stimulated tsh levels were determined after one, two and four weeks. a gradual increase of the mean basal and trh stimulated tsh plasms concentration could be observed. however, after four weeks the trh test was still negative in two patients and weak positive in another case. t3 plasma levels were lowest ... | 1976 | 819709 |
| marked suppression of thyroid function in rats with gram-negative septicemia. | gram-negative septicemia was induced in rats by two daily injections of fecal mixture into the thigh, after which the thyroid function was markedly suppressed for 2 days. iodine metabolism was studied by organ radioassay and by imaging with a multiwire proportional chamber (mwpc) at various time intervals after intravenous injection of 125i. plasma t3, t4, and tsh, measured by radioimmunoassays, were suppressed, as were the t3-resin uptakes. fractional blood supply to the thyroid glands of the i ... | 1976 | 1107490 |
| repression of sporulation in bacillus subtilis by l-malate. | l-malate repressed sporulation in the wild-type strain of bacillus subtilis. when 75 mm l-malate was added to the growth medium at the time of inoculation, the appearance of heat-resistant spores was delayed 6 to 8 h. the synthesis of extracellular serine protease, alkaline phosphatase, glucose dehydrogenase, and dipicolinic acid was similarly delayed. sporulation was not repressed when malate was added to the culture at t4 or later. a mutant was selected for ability to sporulate in the presence ... | 1976 | 812866 |
| starvation-induced alterations of circulating thyroid hormone concentrations in man. | serum concentrations of triiodothyronine (t3), thyroxine (t4), and tsh were examined in seven men and seven women of normal weight during a 60-hr fast. similar studies were conducted in two women who received daily for 1 mo before and during a similar fast, 0.4 mg and 0.5 mg of 1-thyroxine. the serum concentrations of t3 decreased in each of the untreated normal subjects (sign test of significance, p less than 0.001). the mean control concentration of t3 in women was 152 +/- 9 ng/100 ml (x +/- s ... | 1976 | 1246209 |
| results of interstitial curietherapy in t3 and t4 floor of the mouth and tongue cancers after previous arterial infusion chemotherapy. | 1976 | 1246449 | |
| cooperative action of the t4 tail fibers and baseplate in triggering conformational change and in determining host range. | 1976 | 1246820 | |
| conversion of l-thyroxine to triiodo-l-thyronine and biological activity of l-thyroxine, as measured by changes in growth hormone. | pituitary growth hormone (gh) content and plasma gh response to pentobarbital (pb) of severely hypothyroid rats are markedly decreased compared with euthyroid rats. both pituitary and plasma gh may be increased by single ip injections of physiologic doses of thyroxine (t4) (1.75 mug/100g bw) or triiodothyronine (t3) (0.2 mug/100g bw). using this increase in gh of hypothyroid rats to measure the biological effectiveness of single doses of both iodothyronines, we have shown that the administration ... | 1976 | 1248450 |
| thyroid hormone regulation of extrathyroidal iodoproteins. | the effect of thyroid status on plasma and tissue levels of labeled nonextractable iodine (nei) derived from the metabolism of radioiodothyronines was examined in the rat. concentrations of radioiodoprotein were substantially elevated in plasma, kidney, and liver in thyroidectomized animals 72 h postinjection of [125i]triiodothytonine ([125i]t3). similarly, total rat concentrations of radioactive nei were increased (52%) 72 h after injection of [125i]t3. ne125i concentrations from [125i]t3 in pl ... | 1976 | 1248455 |
| a dna polymerase from ustilago maydis. 2. properties of the associated deoxyribonuclease activity. | the polymerase and deoxyribonuclease activities of the purified ustilago maydis dna polymerase coeluted from a hydroxyapatite column, cosedimented in sucrose gradients in both the absence and presence of salt, possessed similar thermolabilities and reaction requirements. these observations suggest that both activities are associated with the same enzyme and that the deoxyribonuclease activity is not a contaminant. the initial rate of degradation of native 3'-end-group-labelled dna was similar to ... | 1976 | 1248476 |
| incorporation of (5-3h) uridine into ribonucleotide pools and rna during thyroxine-induced metamorphosis of xenopus laevis tadpoles. | incorporation of [3h] uridine into the ribonucleoside triphosphates utp and ctp, total rna, and nuclear and cytoplasmic rna was followed in xenopus laevis tadpole liver during thyroxine (t4)-induced metamorphosis. pool sizes of utp and ctp were found to remain unchanged, although turnover the ribonucleoside triphosphates was found to be greatly stimulated after 4 days of hormone treatment. the time course of labeling of the 40-s pre-rrna was very similar to that of utp in both thyreostatic and t ... | 1976 | 1248666 |
| pituitary-thyroid axis reaction after myocardial infarction. | within the first 36 hours following myocardial infarction, serum total thyroxine (t4) levels were supranormal in most cases in contrast to normal thyrotropin values. after one week, t4 levels dropped to normal while tsh values rose significantly. these findings suggest that, in the acute phase of myocardial infarction, the secretion of thyroid hormones is increased, thereby inhibiting the pituitary thyrotropes. the stimulation of thyroid secretion might be due to the high levels of blood catecho ... | 1976 | 1248789 |
| hyperthyroidism with normal concentrations of total serum thyroxine and triiodothyronine. | normal concentrations of total serum thyroxine (t4) and triiodothyronine (t3) were found in a patient who was hyperthyroid because of a hyperfunctioning thyroid adenoma. after surgical removal of the adenoma, the patient became clinically euthyroid; the abnormally high free thyroxine (ft4), triiodothyronine resin uptake (t3r) and rapid achilles reflex time (art) returned to normal. a low-normal concentration of thyroxine binding globulin (tbg) determined by polyacrylamide gel electrophoresis and ... | 1976 | 1249194 |
| total and free thyroxine and triiodothyronine in normal and complicated pregnancy. | serum concentrations of total and free thyroxine (t4 and ft4) and total and free triiodothyronine (t3 and ft3) were measured in normal pregnant women, in patients with toxemia of pregnancy, and in patients with gestational trophoblastic disease (gtd). in normal pregnancy, ft4 and ft3 levels remained normal while t4 and t3 levels were elevated. in patients with pre-eclampsia, the mean serum t3 concentration was significantly lower than that of normal pregnancy and the serum ft3 concentrations in ... | 1976 | 1249198 |
| [unusually high iodine excretion in hyperthyroidism in an iodine-poor area]. | in 27 thyrotoxic patients and in 177 healthy controls from the area of essen we studied the total urinary iodine excretion. whereas the iodine excretion was only 43.7 +/- 27.3 mug iodine per g creatine in the controls, the corresponding average value was significantly higher in thyrotoxic patients. it amounted to 193.3 +/- 201.7 mug iodine per g creatinine. this finding can not be explained by selection of patients with autonomous adenomas and contamination with iodine. possible causes are an in ... | 1976 | 1251155 |
| hormonal effects on the morphology of bone defect healing. | standard defects were produced in the radii of hypophysectomized immature rats. all animals were injected with methimazole, and grouped according to hormone supplementation, growth hormone (gh), thyroxine (t4), the combination, or no hormones. lack of gh and t4 caused a retardation of defect healing and callus formation but not a total cessation. the presence of gh in the hypophysectomized animal caused a larger callus due to greatly increased chondrogenesis throughout the 6 week study. thyroxin ... | 1976 | 1253491 |
| immunogenic properties of bacteriophage spo1 and t4 dna photooxidized in the presence of methylene blue, irradiated with ultra-violet light or containing 5-bromdesoxyuridine. | 1976 | 1254318 | |
| triiodothyronine and thyroxine in urine. i. measurement and application. | urinary triiodothyronine (t3) and thyroxine (t4) were measured by ria, and t4 was also measured by competitive protein binding (cpb). ph 1-hydrolysable conjugates were 48% of total urinary t3, and enzyme- or ph 1-hydrolysable conjugates were 55% and 61% of total urinary t4. the mean unconjugated t3 excretion was 34.3 ng/h (0.99 mug t3/g creatinine) in normal subjects (no day-night rhythm found), 1.56 mug/g in late pregnancy, 0.82 mug/g in neonates (1-12 days), and was also unchanged in persons w ... | 1976 | 1254690 |
| triiodothyronine and thyroxine in urine. ii. renal handling, and effect of urinary protein. | mean urinary clearances of t3 were 164 ml/min in normal subjects, 177 in pregnancy, 221 in thyrotoxicosis, 174 in hypothyroidism, and 194 in 3 persons with undetectable t4 but normal t3 levels. t4 clearances were 38 ml/min in normal subjects, 48 in thyrotoxicosis, and 138 in hypothyroidism. low creatinine clearance was associated with low clearances of t4 and t3. the data suggest urinary excretion of t3 by glomerular filtration of serum unbound t3 with added tubular excretion; and t4 excretion b ... | 1976 | 1254691 |
| measurements of free thyroxine: comparison of per cent of free thyroxine in diluted and undiluted sera. | the effect of dilution on values of the per cent of free t4 in serum (%ft4) measured by equilibrium dialysis, was determined by comparing the values for %ft4 in diluted and undiluted specimens. the specimens studied were sera obtained from 10 normal subjects, 10 patients with hyperthyroidism, 5 with hypothyroidism, and 9 women with normal pregnancy. the method of analysis employed was that described by sterling and brenner, except that the 125i-thyroxine used was predialyzed to remove non-thyrox ... | 1976 | 1254695 |
| recovery of the accumulation ability of thiomethyl-beta-galactoside in escherichia coli after bacteriophage t4 infection. | effects of uv-irridiated and unirradiated t4 phage infection on the beta-galactoside accumulation ability in eschericia coli have been examined by the use of 14c-labeled thiomethyl-beta-galactoside (tmg). under conditions where a synchronous adsorption of phage takes place, the cellular ability for tmg accumulation is found to be largely inhibited immediately after phage adsorption, but it recovers with time to a new level, which is dependent on the multiplicity of infection. when cells are infe ... | 1976 | 1255845 |
| gene 32 protein of bacteriophage t4 moderates the activities of the t4 gene 46/47-controlled nuclease and of the escherichia coli recbc nuclease in vivo. | genes 46 and 47 of phage t4 control a nuclease that is required for genetic recombination and may act similarly to the escherichia coli recbc nuclease. in vivo, the nucleolytic activities of both of these nucleases must be moderated so that recombining dna intermediates are not destroyed. we conclude from our present experiments that the phage t4 gene 32 protein, specifically its c-terminal domain, participates in such moderation. we have investigated dna degradation in different gene 32 and gen ... | 1976 | 1255856 |
| late events in t4 bacteriophage production. i. late dna replication is primarily exponential. | the possibility of a switch in the mechanism of t4 dna replication, from an exponential-bidirectional mode at early stages to a nonexponential (rolling circle) mode at later stages of phage development, has been investigated. the conclusion that dna replication does not involve such a change in mechanism for the majority of replicating molecules is based on the analysis of the clonal distribution of mutants specifically induced at late times after infection. the clonal distribution of mutants, i ... | 1976 | 1255858 |
| late events in t4 bacteriophage production. ii. giant bacteriophages contain concatemers generated by recombination. | analysis of "giant" phage, which package concatenated dna into their capsids, shows that they are predominantly heterozygous. the results are compatible with the hypothesis that concatemers are generated by recombination. | 1976 | 1255859 |
| head morphologies in bacteriophage t4 head and internal protein mutant infections. | escherichia coli infected with phage t4 mutants defective in synthesis of the three major internal proteins found in the phage head, ipi-, ipii-, ipiii-, or ip degrees (lacking all three) were examined in the electron microscope for head formation. infection with ipi- or ipii- does not appear to induce increased aberrant head formation, whereas ipii- or ip degrees infections result in production of polyheads and viable phage. multiple mutants of the early head formation genes 20, 21, 22, 23, 24, ... | 1976 | 1255864 |
| t4 gene 32 protein model for control of activity at replication fork. | limited hydrolysis of gene 32 protein by various proteinases results in the production of three stable cleavage products. two of these products show an affinity for native t4 dna cellulose that the uncleaved protein does not exhibit. a model for proteolytic cleavage and for the total unwinding of dna in advance of the replication fork is discussed in terms of this unusual binding affinity. | 1976 | 1256542 |
| production and evaluation of high-quality thyroxine antisera for use in radioimmunoassay. | high-quality thyroxine (t4) antisera were raised in rabbits. conjugates of protein and t4 or t4 methyl ester were given intracutaneously in freund's complete adjuvant at one or two sites on the back at 1/2- to 3-month intervals. eleven of 16 immunized rabbits produced antiserum with titers higher than 2 commercial antisera and 4 antisera from other laboratories. all 16 antisera had equilibrium constants (k) higher than the 6 reference sera. the highest titer observed was 130,000 with 88 fmol lab ... | 1976 | 1257697 |
| [results of treatment for laryngeal carcinoma in würzburg]. | from 1960 through 1972, altogether 418 patients with laryngeal carcinoma have been treated at würzburg, 333 = 82% of these by primary radiation therapy with co-60 gamma-rays. the results are reported and analyzed in dependence on prognostic factors as tumor site and extension, formation of metastases and age of the patient. five-year survival of primarily irradiated cases from 1960 to 1967; glottic t1 = 90%, t2 = 70%, t3 -31%. supraglottic t2 = 51%, t3 =20%, t4 = 16%. quota of cases with posteri ... | 1976 | 1258083 |
| a new combined thyroid hormone index based on thyroxine, triiodothyronine and resin uptake. | a new thyroid hormone index, based on measurements of thyroxine (t4), triiodothyronine (t3) and resin uptake (rt3u), is proposed. in contrast to previous thyroid hormone indices, it incorporates both t3 and t4 measurements. this combined thyroid hormone index appears to have superior predictive value in terms of clinical correlation with thyroid disease. | 1976 | 1258827 |
| thyroid-pituitary response to cardiopulmonary bypass. | the effects of open heart surgery on various parameters of thyroid-pituitary function were studied in 11 patients. the pattern of hormonal changes was variable. t4 concentration increased during anaesthesia and bypass, following which it returned to normal once haemodynamic stability had been regained. the pattern of change in t3 concentration did not follow consistently that of t4 and the proportional increase in concentration was not as great. there was a significant reduction in t3 concentrat ... | 1976 | 1259888 |
| t4-endonuclease v-sensitive sites in dna from ultraviolet-irradiated human cells. | human diploid cells (wi38) were pre-labeled with 32pi, exposed to ultraviolet irradiation and then pulse labeled with [3h]thymidine. the extracted dna from these cells was subsequently treated with the t4-endonuclease v, an enzyme which specifically nicks dna strands at positions adjacent to pyrimidine dimers. sedimentation in alkaline sucrose gradients revealed that the dna synthesized after irradiation, as well as that made before, contained endonuclease-sensitive sites. our results suggest th ... | 1976 | 1259981 |
| purification of thyroxine and triiodothyronine for radioassay with sephadex. | the use of [125i] thyroxine in the determination of dialysable thyroxine (free t4) places very high demands on the purity of the reagent. less than 5% dissociated radioactive iodine (as iodide) is enough to influence the results [1]. for this reason several workers have developed methods for the purification of iodo amino acids used in radioassay for thyroid hormones. two techniques have been used mainly, namely dialysis of incubated tracer-serum mixture [1,2], and adsorption chromatography on d ... | 1976 | 1261035 |
| the influence of thyroxine on the serum free triiodothyronine concentration. | by means of measurement of the serum free triiodothyronine fraction (%ft3) by equilibrium dialysis, the influence of varying serum concentrations of thyroxine (t4) on %ft3 was studied in conditions associated with lack or excess of serum t4 and in serum enrichment with t4 in vitro. the enrichment of pooled serum with t4 caused significant increases of %ft3 with t4 was added to elevate its t4 content between 10 and 25 mug/dl. in 4 subjects with primary hypothyroidism treated with t3 (25 mug once ... | 1976 | 1261041 |
| a granule-associated l-thyroxine deiodinating system in the human leukocyte. | recent work has shown that leukocytes deiodinate l-thyroxine (t4) and that deiodination is enhanced when phagocytosis is induced. to define the subcellular locus and characteristics of this phenomenon, the deiodinative activities of different cell fractions obtained from human leukocytes (greater than 90% polymorphonuclear) were studied. of the several fractions obtained, the granule fraction was found to possess the bulk of t4-deiodinative activity; as little as 9 mug/ml of granule protein effe ... | 1976 | 1261501 |
| abnormal thyroid function in spontaneously hypertensive rats. | thyroid weight, thyroidal radioiodide uptake, and cyclic amp-dependent protein kinase activity of a thyroid supernatant fraction were increased significantly in spontaneously hypertensive rats (shr), apparently because of increased secretion of pituitary tsh. however, the thyroids of shr did not make supernormal amounts of thyroxine (t4), and thyroidal radioiodine release was apparently impaired. in the shr, proteolytic enzyme activity was less than normal and the thyroglobulin was more resistan ... | 1976 | 1261506 |
| thyroid hormone metabolism during liver regeneration in rats. | the metabolism of thyroid hormones was studied during the prereplicative period of liver regeneration. after partial hepatectomy, serum thyroxine (t4) and triiodothyronine (t3) levles progressively fell, and reached a nadir at 12 h proportional to the quantity of liver tissue exised. the diminution (60-80%) in serum iodothyronines was related specifically to partial hepatectomy because laparotomy, ether anesthesia, and other stressful surgical procedures did not induce similar changes. at least ... | 1976 | 1261517 |
| thyroidal iodine metabolism during the development of the chick embryo. | stable iodine was measured in the thyroid gland of the chick embryo from day 9 to day 20 of incubation in order to evaluate quantitatively the functional development of the gland. total iodine content increased progressively during incubation. from day 9 to day 17 of incubation, this increase resulted from the increases of pellet-bound iodine and of soluble iodine. afterwards, it essentially paralleled the increase of the soluble thyroglobulin-bound iodine which reflected the increase in both th ... | 1976 | 1261525 |
| improved diagnosis and management of hyper- and hypothyroidism by timing the arterial sounds. | "sphygmo-recording," a non-invasive method for timing the arterial pulse wave contour provides an objective measure of responses to medication in patients with hyper- and hypothyroidism. the qkd interval, i.e., the interval from the onset of the qrs complex (q) to the onset of the korotkoff sounds (k) at the brachial artery when the sphygmomanometer cuff is at diastolic pressure (d) is the qkd interval. qkd is normally 205 +/- 12 msec. in the hyperthyroidism the qkd interval may be shortened to ... | 1976 | 1262433 |
| triiodothyronine (t3)-binding immunoglobulins in a euthyroid woman: effects on measurement of t3 (ria) and on t3 turnover. | a 36-year-old woman with nodular goiter, nervousness, and tachycardia was evaluated for t3 toxicosis. her serum thyroxine level, resin t3 uptake, and thyroidal radioiodine uptake were normal. her t3 (ria), by a technique employing charcoal to separate bound and free t3, was reported as indeterminate due to an interfering substance; by a double-antibody method, her t3 (ria) was 325 ng/dl. further studies of the patient's serum revealed an abnormal t3-binding protein which misgrated in the beta-ga ... | 1976 | 1262440 |
| a simple and rapid radioimmunoassay of triiodothyronine in unextracted serum. | a simple, accurate, and rapid procedure for radioimmunoassay (ria) of triiodothyronine (t3) is described. serum is denatured by sodium trichloroacetate and the t3 released is allowed to react with 125i-t3-labeled t3 anti-serum. the displaced 125i-t3 is rapidly taken up by an anionic-resin sponge, and this uptake is linearly related to serum t3 concentration. the entire assay procedure was completed in the same tube in about 1 hr. the t3 antibody was specific, and t4-to-t3 conversion in the assay ... | 1976 | 1262950 |
| thyroxine and triiodothyronine influence cultured throid cells: alterations of morphology and mucosubstances. | further experimentation with the steer thyroid cell line indicates that the formation of "follicles" is enhanced by exposure to 8.9 x 10(-7) m thyroxine (t4) or triiodothyronine (t3) for 96 hr. the production of mucosubstances by the cultured thyroid cells is also increased by treating with t4 or t3, effects demonstrable after staining with pas-alcian blue at ph 2.5. it is suggested that these in vitro effects bear on morphological organization and synthetic activity of the thyroid gland as well ... | 1976 | 1265068 |
| inhibitory effect of large doses of propylthiouracil and methimazole on an increase of thyroid radioiodine release in response to thyrotropin. | thyroidal radioiodine release increased shortly after a single injection of small doses of ptu, while moderate doses of mmi produced a similar increase of thyroidal radioiodine release with a latency of 7-9 hr. large doses of ptu and mmi failed to augment thyroidal radioiodine release for at least 29 to 34 hr after the initial administration of goitrogens, although plasma tsh increased significantly because of goitrogen administration. an increase of thyroid hormone release in response to exogen ... | 1976 | 1265085 |
| ligandin and z protein in binding of thyroid hormones by the liver. | sephadex g-100 chromatography of rat liver supernatant after addition of [125i]t3 revealed four peaks of protein-bound radioactivity in the void volume, albumin, ligandin, and z-containing regions, respectively. the peaks were identified by cochromatography of bsp and [125i]t3 and immonodiffusion with antiratligandin igg and antirat z igg. binding of [125i]t4 to rat liver supernatant occurred in void volume, albumin, and z regions only. studies in vivo reveal a pattern of [125i]t3 binding to rat ... | 1976 | 1267008 |
| preparation of 125i-thyroxine and 125i-triiodothyronine of high specific activity for the radioimmunoassay of serum total t4 and t3. | a simple and inexpensive procedure for the preparation of high specific activity 125i radioiodinated triiodothyronine and thyroxine is described. the specific activities achieved are approximately 350 ci/g for 125i-t4, and either 200 ci/g for 125i-t3 depending on the reagents employed. the high specific activity 125i-t4 and 125i-t3 are suitable for the routine radioimmunoassay of serum total t3 and t4. when properly stored they have a self life of at least seven weeks. | 1976 | 1267413 |
| novel mechanism of resistance to folate analogues: ribonucleoside diphosphate reductase deficiency in bacteriophage t4. | some spontaneously occurring bacteriophage t4 mutants (far mutants) were able to form plaques in the presence of concentrations of folate analogues that completely inhibit plaque formation by wild-type phage t4. some of these far mutants were shown to be ribonucleoside diphosphate (rdp) reductase (ec 1.17.4.1) deficient, and some independently isolated rdp reductase-deficient mutants (nrd mutants) were shown to be folate analogue resistant. the map positions of the rdp reductase-deficient far mu ... | 1976 | 1267427 |
| [cooperative binding of the protein of gene 32 of phage t4 with single-stranded dna]. | 1976 | 1269390 | |
| gamma-ray mutagenesis in bacteriophage t4. | 137cs-gamma irradiation of bacteriophage t4 induces large deletions plus a variety of types of point mutations. all mutations arise with single-hit kinetics, and all by a misrepair process. the estimated point mutation rate is 1.5 x 10(-9) per locus per rad. | 1976 | 1269910 |
| nucleic acid helix-coil transitions mediated by helix-unwinding proteins from calf thymus. | we have studied nucleic acid double helix destabilization mediated by purified calf helix-unwinding proteins, measuring ultraviolet hyperchromicity to detect helix melting. both calf unwinding protein 1 (up1) and a high salt eluting protein fraction are found to depress strongly the helix melting temperature (tm) of the synthetic alternating copolymers poly[d(at)] and poly[r(au)], indicating that both dna and rna are recognized by these proteins. up1 also destabilizes natural, gc-containing dna ... | 1976 | 1270426 |
| single-stranded dna structure and dna polymerase activity in the presence of nucleic acid helix-unwinding proteins from calf thymus. | in the preceding articles we have described the isolation and some of the properties of two calf thymus proteins which bind selectively to single-stranded dna and which appear analogous to previously isolated prokaryotic dna-unwinding proteins. in the present work we demonstrate two further points of analogy. first, both the calf up1 and the high salt eluting proteins form protein-rich complexes with single-stranded dna, and hold this dna in a rigid, extended conformation. second, these proteins ... | 1976 | 1270427 |
| volume of sella turcica in normal subjects and in patients with primary hypothyroidism and hyperthyroidism. | in an attempt to assess a possible relationship between pituitary size and tsh secretion, the volume of sella turcica was measured in 570 subjects, 26 primary hypothyroid patients, and 34 thyrotoxic patients. the volume of sella turcica, measured by a 3-dimensional approach, increased progressively with age until 20 years of age and was rather constant thereafter in normal subjects. in thyrotoxic patients, the volume of sella turcica was normal in spite of decreased plasma tsh concentration. in ... | 1976 | 1270575 |
| effects of postnatal thyroxine administration on brain development, response to postnatal androgen and thyroid regulation in female rats. | some developmental and functional manifestations of thyroxine (t4) administered on the first 2 days of postnatal life were studied in the female rat. brain myelinogenesis estimated by brain esterified cholesterol concentration, and brain myelin age estimated by brain total cholesterol concentration, were subsequently determined. thyroxine treatment resulted in a greater concentration of esterified cholesterol in the brain that saline treatment, but the latter appeared to delay the normal increas ... | 1976 | 1270961 |
| stability of radiothyroxine plasma disappearance curve despite catharsis and unblocked thyroidal uptake of radioiodide. | plasma radioactivity was measured over 21 days after an intravenous injection of 50 muci of 125i-t4 in eight normal men. no thyroid-blocking medication was given. four subjects (castor oil group) received 30 ml of castor oil on each of days 13, 14, and 15, while the other four subjects (control group) were studied without medication. after a 5-day equilibration period, plasma 125i-t4 was measured on days 5-13 in order to calculate the disappearance curve for each subject and to derive the mean f ... | 1976 | 1271113 |
| studies on temperature-dependent ultraviolet light-sensitive mutants of bacteriophage t4: the structural gene for t4 endonuclease v. | 1976 | 1271461 | |
| primary structure of bacteriophage t4 internal protein ii and characterization of the cleavage upon phage maturation. | 1976 | 1271467 | |
| multiple structures of adeno-associated virus dna: analysis of terminally labeled molecules with endonuclease r-hae iii. | the double-stranded form of adeno-associated virus (aav) dna has about 20 sites sensitive to endonuclease r.hae iii from haemophilus aegypitus; the fragments produced fall into about 13 size classes, 8 of which contain single fragments. the location of the hae iii-produced aav fragments relative to the three ecor1 fragments was determined. using revised figures for the molecular weights of the hae iii cleavage products of phix174 replicative form dna, we calculated that aav dna contains about 4, ... | 1976 | 1271522 |
| method for the isolation of bacteriphage t4 mutants that produce particles with giant capsids. | an efficient procedure is described for isolating bacteriophage t4 mutants that produce particles with giant capsids. the selective parameters of the technique rely primarily on the giants' characteristic density in cesium gradients and on their resistance to uv radiation. | 1976 | 1271524 |
| absence of interparental recombination in multiplicity reconstitution from incomplete bacteriophage t4 genomes. | interparental recombination between injected t4 dna molecules is indetectable for incomplete petite phages (carrying a terminally deficient genome and therefore unable to circularize) as well as for genetically complete phages. the nonvialbe petite phages can individually replicate their dna repeatedly, and they aso undergo multiplicity reconstitution, producing complete phages, provided that a host bacterium is infected by several petite particles that carry genetically complementary segments o ... | 1976 | 1271533 |
| identification of gene products required for in vitro formation of the internal peptides of bacteriophage t4. | in vitro formation of both bacteriophage t4 internal peptides (ii and vii) from preexisting precursor protein was shown to require the product of t4 gene 21. the proteolytic factor was detectable in extracts of cells infected with certain phage mutants blocked in early steps of head assembly but could not be demonstrated in extracts of t4 wild-type infected cells. this finding suggests that the proteolytic factor is inactivated during normal phage assembly. the product of t4 gene 22 appears to b ... | 1976 | 1271534 |
| evidence for a third type of uv. repair in bacteriophage t4. | 1976 | 1272269 | |
| [congenital disorders of the thyroid gland in experimental litters obtained from mothers subjected to immunization and the action of i-131]. | i131 injected to rabbits immunized with homologous thyroid gland on the 19th-20th day of pregnancy led to congenital disturbances of the thyroid gland in the experimental litters in the i and ii generations. the functional activity and the activity of proteolytic enzymes of the thyroid gland decreased by the 30th day of the postnatal development. there was revealed a distinct correlation between the content in the thyroid gland of the experimental litters of dit + mit and of organic forms--t3 + ... | 1976 | 1273050 |
| the distribution of functional bacteriophage t4 mrna on polysomes. | 1976 | 1275516 | |
| an evaluation of three commercially prepared anion-exchange resin columns for separation of tetraiodothryonine in serum. | three different commercially prepared anion-exchange resin columns for thyroxine (t4) separation from serum for the "t4 by column" assay were evaluated using the protocol of the supplier. the claims by the suppliers for the distribution and recovery of t4 in the first and second thyroxine-containing eluates were experimentally evaluated by the addition of a tracer quantity of purified [125i]t4. the average experimentally determined elution ratios of recoverable t4 (sum of t4 in first and second ... | 1976 | 1277558 |
| serum triiodothyronine levels in patients receiving l-thyroxine. | serum triiodothyronine (t3) and thyroxine (t4) levels were measured in 103 adult patients receiving sodium l-thyroxine (synthroid), 0.2 or 0.3 mg daily for at least 3 mo, as suppression or replacement therapy. all were judged to be clinically euthyroid at the time of the measurements. the mean +/-sd t3 level during treatment was 130.3 +/-47.5 ng% and the mean t4 level was 11.0 +/-2.0 mug%. in normal untreated euthyroid subjects, the mean t3 level was 115.4 +/-26.0 ng% and the mean t4 level was 8 ... | 1976 | 1277707 |
| the renal handling of insulin and thyroid hormones in normal man. | 1. the renal extraction of insulin and thyroid hormones t3 and t4 was studied in man by right renal vein catheterization. 2. the mean renal extraction of insulin was 0-46, suggesting that the right renal vein should be utilized in comparable studies. 3. no renal extraction of thyroid hormones was detected. | 1976 | 1277752 |
| [enzymatic probing of structural defects induced by u.v. light in phage t4 dna]. | 1976 | 1277994 | |
| host membrane lipid synthesis is not required for successful phage t4 infection. | 1976 | 1108415 | |
| a physiological role for trna nucleotidyltransferase during bacteriophage infection. | bacteriophage infection of e. coli cells deficient in the enzyme trna nucleotidyltransferase (cca mutants) resulted in greatly decreased production of viable progeny phage compared to wild type cells. this decrease amounted to as much as 90% in the case of t-even bacteriophages, and 50-65% for t-odd bacteriophages. however, infection by the rna phages, qbeta and f2, was unaffected by the cca mutation. examination of t4 infection of cca hosts indicated that phage development proceeded normally, t ... | 1976 | 15625866 |
| a comparison of thyrotropin- and dibutyryl cyclic amp- induced thyroglobulin iodination in cultured human thyroid cells. | the relative degree of 125-i labelling of thyroglobulin-- bound mono-iodotyrosine (mit) and di-iodotyrosine (dit) in isolated, cultured human thyroid cells has been compared following exposure of 125-i supplemented cells to 100 mu/ml of bovine thyrotropin (tsh) or 1.0 mm dibutyryl cyclic amp (dbcamp) for 96 hours. pronase digestion of the lysed cells and sephadex g-10 fractionation of the digested lysates revealed a predominance of [125-i]mit over [125-i]dit in both sets of experimental cells as ... | 1976 | 15625879 |
| electroencephalographic and reaction time asymmetries to musical chord stimuli. | eeg was recorded over left and right hemispheres at temporal leads (t3, t4) referred to the vertex (cz) in 16 right-handed male subjects. musical chords were presented randomly in monaural sequence, during a task which required a selective motor response to stimuli presented to one ear. the integrated amplitude of the 8-13 hz alpha rhythm was measured when subjects listened passively. under all conditions, a lower mean alpha amplitude was recorded over the right hemisphere than the left, regardl ... | 1976 | 14677584 |
| molecular and genetic recombination of bacteriophage t4. | most genetic characteristics of t4 recombination (the effects of chromosomal termini on recombination, heterozygosity, high negative interference, base mismatch repair, polarized segregation, and the stimulation or depression of recombination in response to phage mutations or external perturbations) can be expressed adequately in terms of our present understanding of the molecular events of t4 infection cycles. t4 dna replication begins and ends with linear chromosomes and does not require a cir ... | 1975 | 1108766 |
| methods for determining the conversion of l-thyroxine (t4) to l-triiodothyronine (t3)1. | 1975 | 1110639 |