Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| seasonal acclimatization of brain lipidome in a eurythermal fish (carassius carassius) is mainly determined by temperature. | crucian carp (carassius carassius) is an excellent vertebrate model for studies on temperature adaptation in biological excitable membranes, since the species can tolerate temperatures from 0 to +36 degrees c. to determine how temperature affects the lipid composition of brain, the fish were acclimated for 4 wk at +30, +16, or +4 degrees c in the laboratory, or seasonally acclimatized individuals were captured from the wild throughout the year (temperature = +1 to +23 degrees c), and the brain g ... | 2008 | 18305022 |
| improved normalization of real-time reverse transcriptase polymerase chain reaction data using an external rna control. | no golden standard exists for normalization of real-time reverse transcriptase polymerase chain reaction (rt pcr) data and procedures used are often not validated. numerous studies have indicated that current approaches are inadequate. here, we report the development of an external rna control approach. it is the first to add external rna to tissue on a per unit weight basis, and we demonstrate its accuracy, suitability, and necessity in experiments involving severe physiological challenges. we ... | 2008 | 18294949 |
| evaluation of estrogenicity of sewage effluent and reclaimed water using vitellogenin as a biomarker. | to evaluate the quality of reclaimed water, the estrogenicity of effluent from a sewage treatment plant and of reclaimed water treated with coagulation-sedimentation-filtration using the effluent as raw water was assessed using vitellogenin (vtg) as a biomarker. after a three-week exposure, significant (p < 0.05) induction of vtg occurred in female crucian carp (carassius carassius) exposed continuously to the secondary effluent and reclaimed water with different dilutions (12.5, 25, 50, and 100 ... | 2008 | 18092868 |
| the changes of serum testosterone level and hepatic microsome enzyme activity of crucian carp (carassius carassius) exposed to a sublethal dosage of pentachlorophenol. | pentachlorophenol (pcp), which was reported to be a typical persistent organic pollutant and environmental endocrine disruptor, may cause threat to aquatic species. in this study, serum testosterone concentration, activity of liver microsome ethoxyresorufin o-deethylase (erod) and glutathione s-transferases (gst) of crucian carp (carassius carassius) exposed to pcp for 7 and 15 d, respectively, were examined. the results showed that testosterone concentration was induced remarkably after 7 d (p< ... | 2008 | 18063032 |
| predator-induced morphology enhances escape locomotion in crucian carp. | fishes show a remarkable diversity of shapes which have been associated with their swimming abilities and anti-predator adaptations. the crucian carp (carassius carassius) provides an extreme example of phenotypic plasticity in body shape which makes it a unique model organism for evaluating the relationship between body form and function in fishes. in crucian carp, a deep body is induced by the presence of pike (esox lucius), and this results in lower vulnerability to gape-limited predators, su ... | 2008 | 17971327 |
| in vivo characterization of primitive hematopoietic cells in clonal ginbuna crucian carp (carassius auratus langsdorfii). | primitive hematopoietic cells in mammalian bone marrow are purified by flow cytometry using hoechst 33342 (hoechst) and rhodamine-123 (rho), because these dyes efflux activities of hematopoietic cells widely conserved in mammals. hematopoietic stem cells (hscs) are identified as side population (sp) cells, characterized by specific hoechst efflux pattern in flow cytometric analysis. we previously demonstrated that sp cells from teleost body kidney (bk) had the hsc activity by a transplantation e ... | 2008 | 18674821 |
| the goldfish (carassius auratus) as a model for neuroendocrine signaling. | goldfish (carassius auratus) are excellent model organisms for the neuroendocrine signaling and the regulation of reproduction in vertebrates. goldfish also serve as useful model organisms in numerous other fields. in contrast to mammals, teleost fish do not have a median eminence; the anterior pituitary is innervated by numerous neuronal cell types and thus, pituitary hormone release is directly regulated. here we briefly describe the neuroendocrine control of luteinizing hormone. stimulation b ... | 2008 | 18657592 |
| seasonal morphological and biochemical changes of dahlgren cells implies a potential role of the caudal neurosecretory system (cnss) in the reproduction cycle of teleostean fish. | the purpose of this mini-review is to summarize recent research on the seasonal morphological and biochemical changes of dahlgren cells in the caudal neurosecretory system (cnss) of the freshwater teleosts carp carassius auratus. the quantitative proof for these seasonal changes in the morphology and biochemistry of dahlgren cells reflects the relationship between the cnss and the reproduction cycle of fish and implies that the cnss is probably involved in the reproduction process of fish. | 2008 | 18649021 |
| globins and hypoxia adaptation in the goldfish, carassius auratus. | goldfish (carassius auratus) may survive in aquatic environments with low oxygen partial pressures. we investigated the contribution of respiratory proteins to hypoxia tolerance in c. auratus. we determined the complete coding sequence of hemoglobin alpha and beta and myoglobin, as well as partial cdnas from neuroglobin and cytoglobin. like the common carp (cyprinus carpio), c. auratus possesses two paralogous myoglobin genes that duplicated within the cyprinid lineage. myoglobin is also express ... | 2008 | 18537818 |
| oxidative stress and growth inhibition in the freshwater fish carassius auratus induced by chronic exposure to sublethal fullerene aggregates. | the rapid growth of nanotechnology is stimulating research concerning the potential environmental impacts of manufactured nanomaterials. the present study summarizes, to our knowledge, the first examination regarding the potential effects of chronic exposure (32 d) of aquatic organisms (juvenile carp [carassius auratus]) to sublethal concentrations (0.04-1.0 mg/l) of fullerene aggregates (nc60/aq; i.e., c60 suspended in water after long-term stirring) with average diameters of approximately 349 ... | 2008 | 19086321 |
| [collation of data on the ploidy levels and mitochondrial dna phylogenetic lineages in the silver crucian carp carassius auratus gibelio from far eastern and central asian populations]. | the distribution of the diploid and triploid forms and the correspondence between ploidy and mitochondrial dna (mtdna) phylogenetic lineages of the silver crucian carp have been studied in far eastern water bodies and the syr darya river. both diploid and triploid forms have been found in large river systems (the amur, suifun, tumangan, and syr darya river basins). only the diploid form has been detected in lakes of bol'shoi pelis island (peter the great bay of the sea of japan), sakhalin island ... | 2008 | 18767549 |
| [chromosomal studies of germ cells in diploid and polyploid fish produced by distant crossing]. | by observing the chromosomal spreads of germ cells and tissue sections, we studied the chromosomal spreads of germ cells in diploid and polyploid fish produced by distant crossing. the samples covered the second generation of hybrids of red crucian carp (carassius auratus red var.) (female) x common carp (cyprinus carpio) (male) (2n = 100) (f2), allotetraploid hybrids of red crucian carp (female) x common carp (male)(4n = 200), triploid hybrids of gold fish (female)x allotetraploid (male) (3n = ... | 2008 | 18464590 |
| microcystin-induced variations in transcription of gsts in an omnivorous freshwater fish, goldfish. | the glutathione s-transferases are important enzymes in the microcystin-induced detoxication processes. in this experiment, we cloned the full-length cdna of alpha, pi and theta-class-like glutathione s-transferase genes from goldfish (carassius auratus l). their derived amino acid sequences were clustered with other vertebrate alpha, pi and theta-class gsts in a phylogenetic tree and the goldfish gst sequences have the highest similarity with those from common carp and zebrafish. goldfish were ... | 2008 | 18455246 |
| characterization of the cell-mediated cytotoxic responses of isogeneic ginbuna crucian carp induced by oral immunisation with hapten-modified cellular antigens. | a better understanding of the immune responses in fish elicited by oral immunisation is of importance for the development of new and effective oral vaccines for cultured fish. in the present study, we characterized specific cell-mediated cytotoxic responses in isogeneic ginbuna crucian carp (carassius auratus langsdorfii) following oral immunisation with cellular antigens. trinitrophenyl- (tnp) or dinitrophenyl- (dnp) modified syngeneic and allogeneic cells were used for studying the fine specif ... | 2008 | 18407518 |
| sexually disrupting effects of nonylphenol and diethylstilbestrol on male silver carp (carassius auratus) in aquatic microcosms. | based on detected nonylphenol (np) levels in aquaculture water, this study investigated sexually disrupting effects in mature male silver carp (carassius auratus) exposed to np and a positive control diethylstilbestrol (des). the combined evidences of steroid hormone (17beta-estradiol, estrone and testosterone) levels and hispathological pictures showed that np (10 microg/l) and des could exert estrogenic effects through indirect mechanisms [i.e. increased estrogens levels (up to two times) and ... | 2008 | 18325589 |
| gata3 mrna in ginbuna crucian carp (carassius auratus langsdorfii): cdna cloning, splice variants and expression analysis. | gata3, a transcriptional activator, plays a critical role in the development of t-cells and differentiation to t helper type 2 cells. to date, no information is available on the role of gata3 in the teleost immune system. we identified full-length cdna and alternatively spliced variants of ginbuna crucian carp gata3 (gbgata3). the gbgata3 gene is transcribed into multiple splice variants lacking either one or both zinc finger domains, although the sequences of both domains are fully conserved be ... | 2008 | 18313140 |
| characterization and functional analysis of goldfish (carassius auratus l.) tumor necrosis factor-alpha. | we identified and characterized two isoforms of tumor necrosis factor-alpha (tnfalpha) from the goldfish, tnfalpha-1 and tnfalpha-2. at the protein level, goldfish tnfalpha-1 and tnfalpha-2 were most homologous to carp tnfalpha-1 and tnfalpha-2, respectively. phylogenetically, the two goldfish isoforms grouped most closely with the carp tnfalpha isoforms and tnf species of other cyprinids. real-time pcr analysis revealed constitutive expression of goldfish tnfalpha-1 and tnfalpha-2 in all tissue ... | 2008 | 17988738 |
| acute toxicity of synthetic pyrethroid cypermethrin to some freshwater organisms. | ninety-six hours static bioassays were made in the laboratory to determine acute toxicity of cypermethrin to five non-target freshwater organisms belonging to different taxa and niche. susceptibility of the organisms to cypermethrin was in the order: the crustacean diaptomus forbesi > the aquatic insect ranatra filiformis > the freshwater carp cyprinus carpio > the tadpole larva of the toad bufo melanostictus > the oligochaet worm branchiura sowerbyi. ninety-six hours lc50 values of aqueous cype ... | 2008 | 18058051 |
| halquinol modulated growth, physiology, and protein profile and halquinol residue withdrawal study in the indian major carp catla catla (hamilton). | the aim of the present study was to evaluate the effect of halquinol, an antimicrobial used as a growth promotor in poultry, on the fresh water fish catla catla in terms of growth promotion, protein profile, and physiology as the rate of oxygen consumption. a synergic increment in the free amino acid level and total protein concentration suggested enhanced anabolic metabolism resulting in weight gain. when compared with an untreated control group, fishes treated with 0.1% halquinol (t1) showed a ... | 2008 | 19024792 |
| behavioral and respiratory dysfunction in the freshwater fish, labeo rohita (hamilton) under malathion intoxication. | a short term definitive test by static renewal bioassay method was conducted to determine the acute toxicity (lc50) of the commercial grade organophosphate insecticide malathion (50% ec) in the freshwater edible fish, labeo rohita. carp fingerlings were exposed for 96 h to different concentrations (6.0 to 10.1 microl/l) of malathion. the acute toxicity value was found to be 9.0 microl/l and one tenth of lc50 (0.9 microl/l) was selected for sub acute studies. behavioral patterns and oxygen consum ... | 2008 | 19024933 |
| spatiotemporal trends in fish mercury from a mine-dominated ecosystem: clear lake, california. | clear lake, california, usa, receives acid mine drainage and mercury (hg) from the sulphur bank mercury mine, a u.s. environmental protection agency (u.s. epa) superfund site that was active intermittently from 1873 to 1957 and partially remediated in 1992. mercury concentrations were analyzed primarily in four species of clear lake fishes: inland silversides (menidia beryllina, planktivore), common carp (cyprinus carpio, benthic scavenger/omnivore), channel catfish (ictalurus punctatus, benthic ... | 2008 | 19475924 |
| ompa is an adhesion factor of aeromonas veronii, an optimistic pathogen that habituates in carp intestinal tract. | in the present study, we focused on one of the aeromonas veronii isolates that exhibited marked adhesion onto carp intestine and studied its membrane-associated proteins for their possible involvement in mucosal adhesion. | 2008 | 18828791 |
| in vitro search for alternative promoters to the human immediate early cytomegalovirus (ie-cmv) to express the g gene of viral haemorrhagic septicemia virus (vhsv) in fish epithelial cells. | present dna vaccines against fish rhabdoviruses require intramuscular injection (fish-to-fish vaccination) of their g-protein gene under the control of the human immediate early cytomegalovirus (ie-cmv) promoter, while immersion delivery (mass dna vaccination), for instance, by using fish epithelial-specific promoters, would be more practical for aquaculture. to find fish epithelial-specific promoters alternative to the ie-cmv, a comparative study of the effectiveness of different fish promoters ... | 2008 | 18840493 |
| influence of carp intestinal mucus molecular size and glycosylation on bacterial adhesion. | the first step of the pathogenesis of many infectious diseases is the colonisation of the mucosal surface by the pathogen. bacterial colonisation of the mucosal surface is promoted by adherence to high molecular weight mucus glycoproteins. we examined the effect of carp intestinal mucus glycoproteins on the adhesion of different bacteria. the bacteria used were 3 strains of aeromonas hydrophila, and a. salmonicida, edwardsiella tarda and yersinia ruckeri. all bacteria adhered to mucus, but at va ... | 2008 | 18924378 |
| evolution and regulation of the downstream gene of hypoxia-inducible factor-1alpha in naked carp (gymnocypris przewalskii) from lake qinghai, china. | the naked carp (gymnocypris przewalskii) is a native teleost of lake qinghai (altitude, 3.2 km) on the qinghai-tibet plateau in china. hypoxia-inducible factor (hif)-1alpha of gymnocypris przewalskii was cloned and a phylogenetic tree for vertebrate hif-1alpha was constructed. by analysis of maximum likelihood models of codon substitutions for hif-1alpha, three positive sites in the branch lineages of crucian carp, eelpout, and flounder, and a higher proportion of neutral sites in naked carp, an ... | 2008 | 18941827 |
| detection of salmonellae from fish in a natural river system. | sediment, water, and fish gut samples taken at three sites near the headwaters of the san marcos river, texas, were analyzed for salmonellae salmonella spp. by culture and molecular techniques. while enrichment cultures from sediment and water samples from the two uppermost sites were negative for salmonellae in polymerase chain reaction analyses, both sediment and water samples were positive at the downstream site. at all sites, salmonellae were present in the guts of different fishes (e.g., la ... | 2008 | 18942591 |
| the enigmatic role of the ankyrin repeat domain 1 gene in heart development and disease. | it has been proposed that the ankyrin repeat domain 1 (ankrd1) factor (also known as carp) plays a critical role in transcriptional regulation, myofibrillar assembly and stretch sensing during heart development and cardiac insults. ankrd1/carp has also been reported to negatively regulate cardiac gene expression in cell-based promoter-reporter assays. consequently, rapid up-regulation of the ankrd1 gene in myocardium in response to developmental stimuli or pathological insults has tended to be i ... | 2008 | 18956313 |
| plasma biochemical responses of the omnivorous crucian carp (carassius auratus) to crude cyanobacterial extracts. | healthy crucian carp (carassius auratus) were treated by intraperitoneal (i.p.) injection of crude cyanobacterial extracts at two doses, 50 and 200 microg mc-lr equiv kg(-1) bw. high mortality (100%) was observed within 60 h post injection in the high-dose group. in the treated fish, activities of four plasma enzymes, alanine aminotransferase (alt), alkaline phosphatase (alp), aspartate aminotransferase (ast), and lactate dehydrogenase (ldh), all showed substantial increases, with both dose and ... | 2008 | 18958589 |
| annual reproductive phase-related profile of sex steroids and their carrier, shbg, in the indian major carp, labeo rohita. | this study reports annual cyclic changes in the plasma levels of sex hormone binding globulin (shbg), sex steroids (e(2), estradiol; t, testosterone; p, progesterone) and protein (prt) in correlation with reproductive phases in both sexes of an indian major carp, labeo rohita. plasma shbg, e(2), t and prt levels in females and shbg and t levels in males showed steady increases (p<0.005) in parallel with the gonadosomatic index (gsi) escalation in the preparatory and prespawning phases attaining ... | 2008 | 18782576 |
| meprins, membrane-bound and secreted astacin metalloproteinases. | the astacins are a subfamily of the metzincin superfamily of metalloproteinases. the first to be characterized was the crayfish enzyme astacin. to date more than 200 members of this family have been identified in species ranging from bacteria to humans. astacins are involved in developmental morphogenesis, matrix assembly, tissue differentiation and digestion. family members include the procollagen c-proteinase (bmp1, bone morphogenetic protein 1), tolloid and mammalian tolloid-like, hmp (hydra ... | 2008 | 18783725 |
| detecting positively selected codons in the glycoprotein of spring viraemia of carp virus (svcv) isolates from the usa and china. | 2008 | 18801064 | |
| evolution of the inflammatory response in vertebrates: fish tnf-alpha is a powerful activator of endothelial cells but hardly activates phagocytes. | tnf-alpha is conserved in all vertebrate classes and has been identified in all taxonomic groups of teleost fish. however, its biological activities and its role in infection are largely unknown. using two complementary fish models, gilthead seabream and zebrafish, we report here that the main proinflammatory effects of fish tnf-alpha are mediated through the activation of endothelial cells. thus, tnf-alpha promotes the expression of e-selectin and different cc and cxc chemokines in endothelial ... | 2008 | 18802111 |
| dna vaccine protects ornamental koi (cyprinus carpio koi) against north american spring viremia of carp virus. | the emergence of spring viremia of carp virus (svcv) in the united states constitutes a potentially serious alien pathogen threat to susceptible fish stocks in north america. a dna vaccine with an svcv glycoprotein (g) gene from a north american isolate was constructed. in order to test the vaccine a challenge model utilizing a specific pathogen-free domestic koi stock and a cold water stress treatment was also developed. we have conducted four trial studies demonstrating that the psgnc dna vacc ... | 2008 | 18812203 |
| a survey on the faunal diversity of savar upazila, dhaka, bangladesh. | a survey was conducted during january to december 2006 to assess the status of faunal diversity of savar upazila, dhaka, bangladesh. a total of 30 species of birds, 24 species of winter birds, 7 species of reptiles, 3 species of amphibians, 15 species of mammalians and 32 species of fishes were recorded. relative abundance of those species were determined. of the birds, house sparrow (passer domesticus) was abundant while blyth's kingfisher (alcedo hercules), rock eagle owl (bubo bengalensis), h ... | 2008 | 18817158 |
| effect of starvation on transcriptomes of brain and liver in adult female zebrafish (danio rerio). | we used microarray and quantitative real-time pcr (qrt-pcr) analyses in adult female zebrafish (danio rerio) to identify metabolic pathways regulated by starvation in the liver and brain. the transcriptome of whole zebrafish brain showed little response to 21 days of starvation. only agouti-related protein 1 (agrp1) significantly responded, with increased expression in brains of starved fish. in contrast, a 21-day period of starvation significantly downregulated 466 and upregulated 108 transcrip ... | 2008 | 18728227 |
| cloning, characterization and promoter analysis of the common carp igf-ii gene. | insulin-like growth factors (igfs) belong to a family of growth factors with structural homology to proinsulin. up till now, no specific details regarding the transcriptional regulation by autocrine, paracrine or endocrine effector molecules in vivo have been described for the igf-ii gene. this is in big contrast to igf-i gene transcription which has been studied more extensively. to better understand how the igf-ii gene is controlled at the gene transcription level, we have isolated the common ... | 2008 | 18304762 |
| bioaccumulation of hexachlorocyclohexane, dichlorodiphenyltrichloroethane, and estradiol-17beta in catfish and carp during the pre-monsoon season in india. | this investigation was performed to monitor hexachlorocyclohexane isomers (hchs), dichlorodiphenyltrichloroethane (ddt, and its metabolites, refered to as ddts), plasma levels of estradiol-17beta (e2), and the gonadosomatic index (gsi) between sampling sites of unpolluted ponds of gujartal, jaunpur (control site) and the polluted rivers gomti (jaunpur) and ganga (varanasi), which affect the reproductive physiology of some edible catfish and carp during the pre-monsoon season. hchs and ddts were ... | 2008 | 18649020 |
| mechanism of cd2+ on dna cleavage and ca2+ on dna repair in liver of silver crucian carp. | the subject of acute injury, apoptosis and canceration of animals induced by heavy metal ions has been one of the hotspots studied worldwide. however, the exact molecular mechanism of cd-induced carcinogenicity remains largely unclear, and how to relieve the toxicity in vivo has rarely been reported. for this paper, we have investigated the mechanism of cd2+ on dna cleavage and ca2+ on dna repair in the liver of silver crucian carp (carassius auratus gibelio) by agarose gel electrophoresis metho ... | 2008 | 18649022 |
| [effectiveness and characteristics of treating algae-laden raw water by stocking silver carp]. | to reduce the negative effect of algae on conventional water treatment, a full-scale research of removing algae from algae-laden raw water by stocking filter-feeding silver carp was processed. after the pretreatment in a presedimentation tank with silver carp, the concentration of phytoplankton, the biomass of cyanobacteria and microsystis flos-aquae in algae-laden raw water with microsystis flos-aquae its dominant species decreased 61.8%, 76.1% and 78.2% respectively. this effective decrease of ... | 2008 | 18649519 |
| expression of cardiac ankyrin repeat protein, carp, in malignant tumors: diagnostic use of carp protein immunostaining in rhabdomyosarcoma. | cardiac ankyrin repeat protein (carp) is highly expressed in cardiac muscles and detectable in normal skeletal muscles. arpp, a close homolog of carp, has been demonstrated to be useful for distinguishing rhabdomyosarcoma from other malignant tumors. however, the carp distributions among malignant tumors have been poorly investigated. here, we analyzed the comprehensive expression of carp in malignant tumors and evaluated its potential use for rhabdomyosarcoma diagnosis. a total of 159 malignant ... | 2008 | 18656235 |
| differential expression of two interferon-gamma genes in common carp (cyprinus carpio l.). | two interferon gamma (ifn-gamma) genes are expressed in immune cells of teleost fish and are potentially implicated in b- and t-lymphocyte responses. ifn-gamma-2 shows structural and functional characteristics to other vertebrate ifn-gamma genes and is associated with t-lymphocyte function. expression profiling shows ifn-gamma-2 upregulation in t-lymphocytes after phytohemagglutinin (pha) stimulation in vitro. unexpectedly, we found ifn-gamma-1, which is structurally different from ifn-gamma-2, ... | 2008 | 18657572 |
| neurosecretory neurons of the nucleus preopticus (npo) express salmon gnrh mrna and show reproduction phase-related variation in the female indian major carp, cirrhinus cirrhosus. | we studied the expression of sgnrh mrna in the neurons of the nucleus preopticus (npo) of the indian major carp, cirrhinus cirrhosus, and their correlation with the reproductive status of the fish. non-radioisotopic in situ hybridization histochemistry protocol employing biotinylated-oligonucleotide probes complementary to salmon gnrh, cichlid gnrh i, catfish gnrh, chicken gnrh ii (from cichlid and catfish), and mammalian gnrh, were applied to the sections through the poa of the female indian ma ... | 2008 | 18664387 |
| use of vitamin c as an immunostimulant. effect on growth, nutritional quality, and immune response of labeo rohita (ham.). | teleost fish lack the enzyme for endogenous synthesis of ascorbic acid (aa), an essential micronutrient for fish. the aim of this study was to examine the effect of higher levels of dietary vitamin c on growth, nutritional quality, and immunomodulation in the indian major carp, rohu (labeo rohita). four groups of l. rohita were fed experimental diets containing either no vitamin c (control) or supplemented with vitamin c at 500 mg kg(-1) (exp-1), 1000 mg kg(-1) (exp-2), or 1500 mg kg(-1) (exp-3) ... | 2008 | 18665463 |
| histopathological and ultrastructural features of koi herpesvirus (khv)-infected carp cyprinus carpio, and the morphology and morphogenesis of khv. | epizootics of koi herpesvirus (khv) cause mass mortalities in koi carp and common carp worldwide. we used a newly developed 'per-gill infection' procedure with live khv, and then conducted detailed histopathological and ultrastructural studies of khv-infected cells including an examination of the morphology and morphogenesis of khv. the primary target of khv was respiratory epithelial cells of the gill lamellae, and release of virions from infected epithelial cells resulted in a systemic infecti ... | 2008 | 18714678 |
| trypanoplasma borreli cysteine proteinase activities support a conservation of function with respect to digestion of host proteins in common carp. | trypanoplasma borreli is an extracellular parasite that is transmitted by a leech vector and is naturally found in the blood of cyprinid fish. high parasitemia and associated severe anemia together with splenomegaly are typical of infection of common carp, cyprinus carpio l. papain-like cysteine proteinases expressed by trypanosome parasites contribute to the pathogenicity of trypanosomes, and are considered an important target for the development of new trypanocidal drugs. t. borreli is a membe ... | 2008 | 18571233 |
| expression of genes involved in excitatory neurotransmission in anoxic crucian carp (carassius carassius) brain. | the crucian carp, carassius carassius, survives months without oxygen. during anoxia it needs to keep energy expenditure low, particularly in the brain, with its high rate of atp use related to neuronal activity. this could be accomplished by reducing neuronal excitability through altered expression of genes involved in excitatory neurotransmission. through cloning and the use of a recently developed real-time rt-pcr approach, with an external rna control for normalization, we investigated the e ... | 2008 | 18593861 |
| effect of botulinum toxin a-induced paralysis and exercise training on mechanosensing and signalling gene expression in juvenile rat gastrocnemius muscle. | intramuscular injections of the paralytic botulinum neurotoxin a (btx) and physical exercise are used in the treatment of chronic spasticity in children with cerebral palsy. we tested whether btx-induced paralysis and/or exercise training would have differential effects on the expression of mechanosensing and signalling genes implicated in the adaptive remodelling of skeletal muscle. juvenile (29-day-old) male rats were injected with btx or saline (nobtx) into the right gastrocnemius and housed ... | 2008 | 18603602 |
| distribution of marine birnavirus in cultured olive flounder paralichthys olivaceus in korea. | surveys of marine birnavirus (mabv) were undertaken in cultured olive flounder paralichthys olivaceus from the south and west coastal areas and jeju in korea during the period january 1999 to april 2007. mabv was detected in all seasons from the fry, juveniles and adult fish from the areas examined. evident cytopathic effects of the virus including rounding and cell lysis were observed in chinook salmon embryo (chse-214) and rainbow trout gonad (rtg-2) cells, but not in fathead minnow (fhm) and ... | 2008 | 18604495 |
| mmts, a new subfamily of tc1-like transposons. | a novel tc1-like transposable element has been identified as a new dna transposon in the mud loach, misgurnus mizolepis. the m. mizolepis tc1-like transposon (mmts) is comprised of inverted terminal repeats and a single gene that codes tc1-like transposase. the deduced amino acid sequence of the transposase-encoding region of mmts transposon contains motifs including dde motif, which was previously recognized in other tc1-like transposons. however, putative mmts transposase has only 34-37% ident ... | 2008 | 18612245 |
| development and evaluation of a one-step loop-mediated isothermal amplification for detection of spring viraemia of carp virus. | spring viraemia of carp virus (svcv) is the causative agent of svc disease. the main aim of our study was to develop a one-step reverse transcription loop-mediated isothermal amplification (rt-lamp) assay for rapid, sensitive and effective detection of svcv. | 2008 | 18631262 |
| genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional orf. | the complete genome of mandarin fish siniperca chuatsi rhabdovirus (scrv) was cloned and sequenced. it comprises 11,545 nucleotides and contains five genes encoding the nucleoprotein n, the phosphoprotein p, the matrix protein m, the glycoprotein g, and the rna-dependent rna polymerase protein l. at the 3' and 5' termini of scrv genome, leader and trailer sequences show inverse complementarity. the n, p, m and g proteins share the highest sequence identities (ranging from 14.8 to 41.5%) with the ... | 2008 | 18068257 |
| [kinetics of hemoglobin carrying and releasing oxygen.]. | the purpose of the present study was to examine the kinetic process of hemoglobin (hb) carrying and releasing oxygen. under the standard conditions (ph 7.4, p(o2) 20 mmhg, 20 degrees c) the blood samples of chicken, rabbit, frog and carp were equilibrated in oxygen content analyzer with calibrated gas mixture a (0.5% co2 and 99.5% n2). then the blood samples were exposed to gas mixture b (21% o2, 0.5% co2 and 78.5% n2). after equilibration, the blood samples were exposed to gas mixture a again. ... | 2008 | 18288362 |
| effect of endotoxin on the immunity of indian major carp, labeo rohita. | endotoxin, a lipopolysaccharide component of outer cell wall membrane of the gram-negative bacteria is a factor responsible for a number of biological effects including immunostimulatory activities in different animal species including fish. in this study, l. rohita yearlings of weight ranging from 80 to 100g were injected intraperitoneally with 0.5, 1, 2, 5, 10 and 20 eu/fish dose of endotoxin to find out its effect on the immunity. the l. rohita yearlings were found to resist the endotoxin dos ... | 2008 | 18289877 |
| molecular cloning and functional characterisation of a cathepsin l-like proteinase from the fish kinetoplastid parasite trypanosoma carassii. | trypanosoma carassii is a fish kinetoplastid parasite that belongs to the family trypanosomatida. in the present study we cloned a cathepsin l-like proteinase from t. carassii. the nucleotide sequence of 1371bp translated into a preproprotein of 456 amino acids. the preproprotein contained the oxyanion hole (gln), the active triad formed by cys, his and asn and the conserved erfnin-like, gnfd and gcngg motifs, characteristic for cathepsin l proteinases. phylogenetic analysis showed that the t. c ... | 2008 | 18162408 |
| the cloning of dmc1 cdnas and a comparative study of its expression in different ploidy cyprinid fishes. | dmc1 (disrupted meiotic cdna) is a functionally specific gene, which was firstly discovered in yeast and then found to encode a protein required for homologous chromosome synapsis during the process of meiosis. in this investigation, we cloned the partial cdnas of dmc1 of diploid red crucian carp, japanese crucian carp, common carp, triploid crucian carp and allotetraploid hybrids by using a pair of degenerate primers based on the conservative sequence of amino acids of the dmc1 protein in yeast ... | 2008 | 18176789 |
| mitochondrial dna analysis reveals cryptic large-scale invasion of non-native genotypes of common carp (cyprinus carpio) in japan. | wild common carp (cyprinus carpio) are probably suffering from biological invasions of conspecific domesticated strains. however, such invasions may be largely camouflaged by morphological similarities between introduced and native strains. we conducted a large survey of mitochondrial dna sequences (complete d-loop region) from 11 localities in japan. from a total of 166 individuals, 28 haplotypes were determined to fit into six divergent clades. one of the six clades included 19 closely related ... | 2008 | 18194166 |
| acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp carassius auratus. | recent evidences suggested that oxidative stress may play a significant role in the pathogenesis of mcs toxicity. in the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mug mc-lreq per kg body weight (bw). we reported the cdna sequences for four kinds of antioxidant enzyme (gsh-px, cat, cu/zn sod, and gr) genes, and evaluated the oxidant stress induced by mcs through analyzing the t ... | 2008 | 18214919 |
| stimulation of salmon calcitonin on secretion of 17beta-estradiol by the ovarian follicles of common carp, cyprinus carpio. | the effects of salmon calcitonin (sct) on the secretion of 17beta-estradiol (e(2)) were examined in female common carp, cyprinus carpio. vitellogenic stage fish adapted to high-ca water were i.p. injected with vehicle, sct, human chorionic gonadotropin (hcg), or hcg plus sct. to determine whether ovarian follicles are equipped with ct receptors, a ct binding assay was conducted. in the in vitro experiments, vitellogenic follicles were incubated with stimulators and inhibitors. administration of ... | 2008 | 18252964 |
| transcriptional deregulation and a missense mutation define ankrd1 as a candidate gene for total anomalous pulmonary venous return. | total anomalous pulmonary venous return (tapvr) is a congenital heart defect in which the pulmonary veins fail to enter the left atrium and drain instead into the right atrium or one of its venous tributaries. although a genetic basis for tapvr has long been recognized, no single gene involved in the pathogenesis of this disease has been identified to date. we previously reported a tapvr patient bearing a de novo 10;21 balanced translocation. in this work, we cloned both translocation breakpoint ... | 2008 | 18273862 |
| long-term hematopoietic reconstitution by transplantation of kidney hematopoietic stem cells in lethally irradiated clonal ginbuna crucian carp (carassius auratus langsdorfii). | the study of hematopoietic stem cells (hscs) has been facilitated by the transplantation of bone marrow cell populations into lethally irradiated mice. it is widely known that hscs have the capacity for long-term and multilineage hematopoietic reconstitution in lethally irradiated hosts. here, we developed a transplantation model system using clonal ginbuna crucian carp (carassius auratus langsdorfii) that were exposed to a lethal dose of x-rays. the minimum lethal dose (mld) of ginbuna was appr ... | 2008 | 18314191 |
| functionality of a bacillus cereus biological agent in response to physiological variables encountered in aquaculture. | the potential of a bacillus cereus isolate (nrrl 100132) as a biological agent for aquaculture has been demonstrated in vitro and in vivo. the functionality of this isolate across a range of physiological conditions, including salinity, ph and temperature, based on rearing of high-value ornamental cyprinus carpio, was investigated. temperature had a significant influence on germination, specific growth rate and increase in cell number of b. cereus in shake-flask cultures, whilst salinity and ph ... | 2008 | 18317748 |
| a liposome immune lysis assay for enrofloxacin in carp and chicken muscle. | a homogenous complement-mediated liposome immune lysis assay (lila) was developed for the determination of enrofloxacin (enro) in carp and chicken muscle. enro was covalently coupled to dppe, and then immobilized onto the surface of liposomes by reverse-phase evaporation method. the performed liposome would be specifically lysed by the sequential additions of anti-enro monoclonal antibody (mab) and guinea pig complement. through a competitive assay format, the performed liposome can be used to d ... | 2008 | 18331861 |
| cloning of the koi herpesvirus genome as an infectious bacterial artificial chromosome demonstrates that disruption of the thymidine kinase locus induces partial attenuation in cyprinus carpio koi. | koi herpesvirus (khv) is the causative agent of a lethal disease in koi and common carp. in the present study, we describe the cloning of the khv genome as a stable and infectious bacterial artificial chromosome (bac) clone that can be used to produce khv recombinant strains. this goal was achieved by the insertion of a loxp-flanked bac cassette into the thymidine kinase (tk) locus. this insertion led to a bac plasmid that was stably maintained in bacteria and was able to regenerate virions when ... | 2008 | 18337580 |
| comparison of antimicrobial activity in the epidermal mucus extracts of fish. | the mucus layer on the surface of fish consists of several antimicrobial agents that provide a first line of defense against invading pathogens. to date, little is known about the antimicrobial properties of the mucus of arctic char (salvelinus alpinus), brook trout (s. fontinalis), koi carp (cyprinus carpio sub sp. koi), striped bass (morone saxatilis), haddock (melanogrammus aeglefinus) and hagfish (myxine glutinosa). the epidermal mucus samples from these fish were extracted with acidic, orga ... | 2008 | 18342561 |
| a 15mb duplication of 6q24.1-q25.3 associated with typical but milder features of the duplication 6q syndrome. | we report on a female patient carrying a de novo 15mb duplication of 6q24.1-q25.3 detected by conventional karyotyping and fine-mapped by molecular karyotyping with a 250k snp array. pure interstitial duplications of 6q are rarely reported in the literature and none of them exactly mapped by array technique so far. our patient shows typical aspects of the "duplication 6q" syndrome such as hypertelorism, downslanting palpebral fissures, carp shaped mouth and joint contractures, but milder mental ... | 2008 | 18342594 |
| identification of envelope protein porf81 of koi herpesvirus. | koi herpesvirus (khv), an emerging pathogen causing mass mortality in koi and common carp, possesses the largest known herpesvirus genome of 295 kbp predicted to encode 156 different proteins. however, none of them has been identified or functionally characterized up to now. in this study, a rabbit antiserum was prepared against a bacterial fusion protein that permitted detection of the predicted type iii membrane protein encoded by orf81 of khv. in western blot analyses, the abundant orf81 gene ... | 2008 | 18343829 |
| detection of spring viraemia of carp virus (svcv) by loop-mediated isothermal amplification (lamp) in koi carp, cyprinus carpio l. | spring viraemia of carp virus (svcv) is a rhabdovirus associated with systemic illness and mortality in cyprinids. several diagnostic tests are available for detection of svcv. however, most of these tests are time consuming and are not well adapted for field-based diagnostics. in this study, a diagnostic tool for svcv detection based on reverse transcription loop-mediated isothermal amplification (rt-lamp) has been developed. based on the nucleotide sequence of the glycoprotein (g) gene of svcv ... | 2008 | 18353016 |
| chemotaxis towards, adhesion to, and growth in carp gut mucus of two aeromonas hydrophila strains with different pathogenicity for common carp, cyprinus carpio l. | characteristics that promote bacterial colonization of the intestinal mucosal surface were examined in two strains of the common fish pathogen aeromonas hydrophila, with different pathogenicity. the characteristics examined were chemotactic activity towards mucus, bacterial adherence to mucus and growth in mucus. intestinal gut mucus of healthy common carp was used. the results indicate that chemotaxis is not necessary for a bacterium to become pathogenic, but it may be a necessary parameter for ... | 2008 | 18355183 |
| isolation and characterization of immunoglobulin of the indian major carp, rohu [labeo rohita (ham.)]. | information on the structure and character of immunoglobulin of fishes is essential in health management. a study was carried out to characterize the serum immunoglobulin (igm) of the indian major carp, rohu labeo rohita (ham.). rohu (500g) were immunised with bovine serum albumin (bsa) and the anti-bsa antibody was purified employing bsa-cl agarose affinity column chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of the purified ig in a 3% gel under non-reduce ... | 2008 | 18375144 |
| development and characterization of monoclonal antibodies to spring viraemia of carp virus. | five monoclonal antibodies (mabs) against spring viraemia of carp (svcv0504, isolated from common carp in china) were produced from mice immunized with purified virus preparations. the virion of svcv contains five structural proteins, representing the nucleoprotein (n), phosphoprotein (p), matrix protein (m), glycoprotein (g) and rna-dependent rna polymerase (l). western blotting analysis revealed that three mabs (1h5, 1e10, and 1h7) recognized specifically to a single protein of 47kda (n), the ... | 2008 | 18378003 |
| carps enhance p53 turnover by degrading 14-3-3sigma and stabilizing mdm2. | carp1 and carp2 proteins (carps) are e3 ligases that target p53 as well as phospho-p53 for degradation. because mdm2 is a critical regulator of p53 turnover, we investigated and found that carps associate with mdm2. we provide evidence that carps stabilize mdm2 by inhibiting mdm2 self-ubiquitination. carps together with mdm2 enhance p53 degradation, thereby inhibiting p53-mediated cell death. carp protein levels correlate with mdm2 levels including under hypoxia where both are reduced. carp2 was ... | 2008 | 18382127 |
| exposure of mother-child and postpartum woman-infant pairs to ddt and its metabolites in tianjin, china. | 1,1,1-trichloro-2,2'-bis(4-chlorophenyl)ethane (ddt) exhibits its long persistence in the environment, unusual bioaccumulation, effects on wildlife, and the possibility of long-term adverse effects on human health, especially reproductive toxicity. despite the prohibition of most persistent organochlorine pesticides in china, the presence of organochlorine residue, including ddt, has been widely indicated in environmental substance. however, scarce information is available about accumulative lev ... | 2008 | 18387653 |
| vitellogenin, a biomarker for environmental estrogenic pollution, of reeves' pond turtles: analysis of similarity for its amino acid sequence and cognate mrna expression after exposure to estrogen. | vitellogenin (vtg), a biomarker for environmental estrogenic pollution, can be detected in the bloodstream of oviparous animals before morphological and functional abnormalities appear due to exposure to environmental estrogens. reports observing vtg in turtles have been limited. we therefore cloned and sequenced a partial cdna of vtg in reeves' pond turtle, chinemys reevesii. the cloned cdna fragment possessed the start codon and 2,229 bp, encoding 743 amino acid residues. a sequence of deduced ... | 2008 | 18388421 |
| pathogenic potential of a collagenase gene from aeromonas veronii. | the role of collagenase as a mechanism of bacterial pathogenicity in some pathogenic bacteria has been reported. the information on the role of collagenase in aeromonas spp. pathogenesis is scant. in the present study, a mutant aeromonas veronii ry001 that is deficient in the putative collagenase gene acg was constructed and compared with the wild-type strain for virulence factors. bacterial cells and cell-free extracellular products of the mutant had significantly less collagenolytic activity, ... | 2008 | 18388966 |
| differential contribution of neutrophilic granulocytes and macrophages to nitrosative stress in a host-parasite animal model. | tyrosine nitration is a hallmark for nitrosative stress caused by the release of reactive oxygen and nitrogen species by activated macrophages and neutrophilic granulocytes at sites of inflammation and infection. in the first part of the study, we used an informative host-parasite animal model to describe the differential contribution of macrophages and neutrophilic granulocytes to in vivo tissue nitration. to this purpose common carp (cyprinus carpio) were infected with the extracellular blood ... | 2008 | 18406465 |
| strain-associated virulence factors of streptococcus iniae in hybrid-striped bass. | streptococcus iniae is a major fish pathogen producing invasive infections that result in economic losses in aquaculture. development of in vitro models of s. iniae virulence may provide insight to the pathogenesis of infection in vivo. three s. iniae strains (k288, 94-426, and 29178) were tested for virulence in a hybrid-striped bass (hsb) model using intraperitoneal injection. s. iniae strains k288 and 94-426 caused high levels of mortality in hsb (lethal dose 2x10(5)cfu) while strain 29178 wa ... | 2008 | 18406547 |
| [use of properties and regulation peculiarities of enzymes of glycogenolysis in fish skeletal muscle depending on peculiarities of motor activity of species]. | levels of activity, properties, and peculiarities of activation of glycogen phosphorylase (gp; ec 2.4.1.1) and glycogen phosphorylase kinase (gpk; ec 2.7.1.38) were studied in the white skeletal muscle of fish differing in motor behavior. no differences in the gp and gpk activity levels were revealed in laskir diplodus annularis (l.), horse mackerel trachurus mediterraneus ponticus, salmon salmo trutta morphario, scorpena scorpaena porcus, scophtalnus maeoticus, and carp cyprinus carpio; however ... | 2008 | 18411509 |
| analysis of dbc1 and its homologs suggests a potential mechanism for regulation of sirtuin domain deacetylases by nad metabolites. | deleted in breast cancer-1 (dbc1) and its paralog carp-1 are large multi-domain proteins, with a nuclear or perinuclear localization, and a role in promoting apoptosis upon processing by caspases. recent studies on human dbc1 show that it is a specific inhibitor of the sirtuin-type deacetylase, sirt1, which deacetylates histones and p53. using sensitive sequence profile searches and hmm-hmm comparisons we show that the central conserved globular domain present in the dbc1 and it homologs from di ... | 2008 | 18418069 |
| production of nitric oxide by carp (cyprinus carpio l.) kidney leukocytes is regulated by cyclic 3',5'-adenosine monophosphate. | the inducible nitric oxide synthase (inos) plays a central role in the inflammatory reactions that follow infection or tissue damage. induction of nitric oxide (no) synthesis by bacterial lipopolysaccharide (lps) depends on activation of g protein-coupled receptors in mammals. thus, it was our intention to evaluate whether similar mechanisms are involved in inos activation in fish leukocytes. therefore, the participation of membrane-bound receptors which activate effectors via g proteins has bee ... | 2008 | 18424130 |
| application of pcr-rf-sscp to study major histocompatibility class ii b polymorphism in common carp (cyprinus carpio l.). | a variety of methods have been applied for the characterization of major histocompatibility (mh) polymorphism in fish. we optimized a technique designated polymerase chain reaction-restriction fragments-single strand conformation polymorphism (pcr-rf-sscp) for screening a large number of individuals for the cyca-dab1 and cyca-dab2 genes polymorphism in common carp. the advantages of this technique are simplicity, high sensitivity and low costs. pcr-rf-sscp analysis revealed different genotypes c ... | 2008 | 18430588 |
| perfluorooctane sulfonate (pfos) and other fluorochemicals in fish blood collected near the outfall of wastewater treatment plant (wwtp) in beijing. | perfluorinated compounds (pfcs) were measured in zooplankton and five fish species collected from gaobeidian lake, which receives discharge from wastewater treatment plant (wwtp) in beijing, china. the mean total pfcs in five fish were in the order: crucian carp>common carp>leather catfish>white semiknife carp>tilapia. perfluorooctane sulfonate (pfos) occurred at the greatest concentrations, with mean concentrations ranging from 5.74 to 64.2 ng/ml serum. perfluorodecanoic acid (pfda) was the sec ... | 2008 | 18439735 |
| meichroacidin containing the membrane occupation and recognition nexus motif is essential for spermatozoa morphogenesis. | meichroacidin (mca) is a highly hydrophilic protein that contains the membrane occupation and recognition nexus motif. mca is expressed during the stages of spermatogenesis from pachytene spermatocytes to mature sperm development and is localized in the male meiotic metaphase chromosome and sperm flagellum. mca sequences are highly conserved in ciona intestinalis, cyprinus carpio, and mammals. to investigate the physiological role of mca, we generated mca-disrupted mutant mice; homozygous mca mu ... | 2008 | 18453535 |
| expression of an adenovirus encoded reporter gene and its reactivation following uvc and oxidative damage in cultured fish cells. | recombinant human adenovirus, adca35lacz, was used to examine expression of a reporter gene and its reactivation following uvc (200-280 nm) and oxidative damage in fish cells. | 2008 | 18470745 |
| extensive expansion and diversification of the chemokine gene family in zebrafish: identification of a novel chemokine subfamily cx. | the chemokine family plays important roles in cell migration and activation. in humans, at least 44 members are known. based on the arrangement of the four conserved cysteine residues, chemokines are now classified into four subfamilies, cxc, cc, xc and cx3c. given that zebrafish is an important experimental model and teleost fishes constitute an evolutionarily diverse group that forms half the vertebrate species, it would be useful to compare the zebrafish chemokine system with those of mammals ... | 2008 | 18482437 |
| family association between immune parameters and resistance to aeromonas hydrophila infection in the indian major carp, labeo rohita. | seven innate immune parameters were investigated in 64 full-sib families (the offspring of 64 sires and 45 dams) from two year-classes of farmed rohu carp (labeo rohita). survival rates were also available from aeromonas hydrophila infection (aeromoniasis) recorded in controlled challenge tests on a different sample of individuals from the same families. due to strong confounding between the animal additive genetic effect and the family effects (common environmental+non-additive genetic), reliab ... | 2008 | 18486488 |
| protein expression by aeromonas hydrophila during growth in vitro and in vivo. | expression of aeromonas hydrophila cellular and extracellular products (ecps) was examined following culture of the bacterium in vitro, in tryptic soy broth (tsb), and in vivo, in dialysis tubing placed within the peritoneal cavity of common carp (cyprinus carpio l.). whole cell (wc), outer membrane proteins (omps) and ecp components of the bacteria were analysed by 1 dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (1d sds-page). additionally, 2d sds-page was used to analy ... | 2008 | 18490132 |
| recombinant novel pituitary adenylate cyclase-activating polypeptide from african catfish (clarias gariepinus) authenticates its biological function as a growth-promoting factor in low vertebrates. | nowadays, the studies of pituitary adenylate cyclase-activating polypeptide (pacap)-related peptide (prp) and pacap in non-mammalian vertebrates, especially in fish, have paid attention mainly to the localization, cloning, and structural evolution of the peptides, but very little is known about its biological functions as growth-promoting factors in low vertebrates. in this work, we have cloned and characterized the prp/pacap cdna from the commercially important north african catfish clarias gar ... | 2008 | 18492822 |
| nitric oxide formation from the reaction of nitrite with carp and rabbit hemoglobin at intermediate oxygen saturations. | the nitrite reductase activity of deoxyhemoglobin has received much recent interest because the nitric oxide produced in this reaction may participate in blood flow regulation during hypoxia. the present study used spectral deconvolution to characterize the reaction of nitrite with carp and rabbit hemoglobin at different constant oxygen tensions that generate the full range of physiological relevant oxygen saturations. carp is a hypoxia-tolerant species with very high hemoglobin oxygen affinity, ... | 2008 | 18494799 |
| transcriptional analysis of the common carp (cyprinus carpio l.) immune response to the fish louse argulus japonicus thiele (crustacea: branchiura). | in the present study we investigated changes in transcription levels of a panel of selected immune relevant genes in peripheral blood leucocytes (pbl) and skin samples collected from carp exposed to larval argulus japonicus. we show that in skin up-regulation of gene transcription of the chemokine cxca, and to a lesser extent the chemokine receptor cxcr1 and the cytokine tnfalpha, are good indicators of parasite-induced skin damage at 2 days post-parasite exposure. up-regulation of gene transcri ... | 2008 | 18502149 |
| cdna expression library screening and identification of two novel antigens: ubiquitin and receptor for activated c kinase (rack) homologue, of the fish parasite trypanosoma carassii. | trypanosoma carassii is a kinetoplastid parasite infecting cyprinid fish with a high prevalence in nature. antibodies have been shown to play a protective role in the immune response against this parasite in common carp, cyprinus carpio. to identify immunogenic and putative protective t. carassii antigens we constructed a lambdatriplex2 expression library of the parasite and screened this with pooled carp immune serum collected 6 weeks post-infection. screening of the library not only revealed r ... | 2008 | 18502151 |
| antibody response and resistance of cyprinus carpio immunized with cyprinid herpes virus 3 (cyhv-3). | cyhv-3 (cyprinid herpesvirus-3) is a large dna virus that causes a fatal disease in koi and common carp. infection with wild type or attenuated virus induces an immune response that renders the fish resistant to further virus challenges. the kinetics and affinity of the antibody response in immune fish depend on the temperature of the water. virus-inoculated fish produce anti-cyhv-3 antibodies, which gradually decrease during 280 days post infection to a level slightly above that of naïve fish. ... | 2008 | 18534721 |
| carp vitellogenin detection by an optical waveguide lightmode spectroscopy biosensor. | a label-free carp vitellogenin sensor has a strong potential for on-site monitoring on the possible contamination of edible fish with endocrine disruptors as a sum parameter in an inland carp farm. in this study, we performed a sensitive detection for carp vitellogenin with a direct-binding optical waveguide lightmode spectroscopy-based immunosensor. carp vitellogenin bound over the sensor surface quite specifically, judging from the sensor responses according to stepwise antibody immobilization ... | 2008 | 18534837 |
| serum crp-like protein profile in common carp cyprinus carpio challenged with aeromonas hydrophila and escherichia coli lipopolysaccharide. | the potential of c-reactive protein (crp)-like proteins to be used as a biomarker of health status in cultured carp obtained from various european fish lines has been assessed. varying crp-like protein levels in the serum of carp were monitored using an indirect competitive enzyme-linked immunosorbent assay. crp-like protein basal levels in normal fish varied between carp lines, ranging on average from 2.9+/-0.15 to 12.57+/-1.19 microg ml(-1). serum levels of crp-like protein in carp were observ ... | 2008 | 18538390 |
| teleostean il11b exhibits complementing function to il11a and expansive involvement in antibacterial and antiviral responses. | interleukin 11 is a class-1 helical cytokine, having the four-helix bundle structure, possessing pleiotropic characteristics involved in physiological processes including blood production, bone formation and placentation. the interleukin 11 paralogues (il11a and il11b) have been identified in fish with only il11a from carp and trout have been characterized and analyzed for its expression thus far. here, we cloned and studied the structure and expression of il11b in japanese flounder (paralichthy ... | 2008 | 18538848 |
| effect of cycloart-24-en-3beta-ol from euphorbia royleana latex on neuroenzyme ache and oxidative metabolism of freshwater fish, channa punctatus. | cycloart-24-en-3beta-ol isolated from euphorbia royleana latex possesses potent piscicidal activity against freshwater predatory fish channa punctatus. their short as well as long-term exposure causes significant (p<0.05) time and dose-dependent reversible alteration in the oxidative metabolism of the fish channa punctatus cycloart-24-en-3beta-ol also shifts fish respiratory pathway, inhibits energy production and ache activity reversibly may be advantageous for their use as environmentally safe ... | 2008 | 20161954 |
| changes in plasma thyroid hormones and cortisol levels in crucian carp (carassius auratus) exposed to the extracted microcystins. | the endocrine response of crucian carp injected intraperitoneally with extracted microcystins (mc) was investigated in this study. fish were injected intraperitoneally either with 0.75% nacl (control) and microcystis extract corresponding to 150 and 600 microg microcystins per kg body weight. the plasma levels of triiodothyronine (t(3)), thyroxine (t(4)), free triiodothyronine (ft(3)), free thyroxine (ft(4)), and cortisol were determined at 0, 1, 3, 12, 24, and 48h post-administration of mc-cont ... | 2008 | 18990424 |
| accumulation of heavy metals in freshwater fish in cage aquaculture at cirata reservoir, west java, indonesia. | freshwater fish demand in west java is supported by cage aquaculture in reservoirs. cirata reservoir is one of three cascading hydropower reservoirs built along the citarum river that receives domestic and industrial wastes. water pollution by heavy metals, such as copper and lead, increases the health risk of humans who consume the fish. the study was aimed to evaluate the concentration of copper and lead in fish, specifically on cyprinus carpio, that are widely cultivated in the cirata reservo ... | 2008 | 18991927 |
| seasonal variations of heavy metals in some organs of carp (cyprinus carpio l., 1758) from beyşehir lake (turkey). | in this study which was carried out between march 2003 and february 2005 fe, cu, zn, mn, cr, pb and cd contents were determined in muscle, liver and gill of carp (cyprinus carpio l., 1758) caught from beyşehir lake. among the heavy metals analyzed cr, pb and cd were below the detection limit (<0.03). heavy metal concentrations varied significantly depending on the type of the tissue and season. the highest metal concentrations were found in the liver, followed by gill and muscle. heavy metal lev ... | 2008 | 17503200 |
| ecotoxicological assessment of water pollution in sariyar dam lake, turkey. | given the effects of environmental pollution and different biotic factors on some important biochemical markers, as enzymes, two fish species inhabiting the sariyar dam lake, turkey have been investigated. ethoxyresorufin o-deethylase, glutathion s-transferase, lactate dehydrogenase, alkaline phosphatase, acid phosphatase, and alanine and aspartate amino transferase activities have been measured in liver samples of cyprinus carpio and capoeta tinca. also, brain acetylcholinesterase and carboxyle ... | 2008 | 17582495 |
| molecular cloning and expression analysis of t-bet in ginbuna crucian carp (carassius auratus langsdorfii). | in the adaptive immune system of mammals, naive helper t (th) cells differentiate into th1 or th2 cells. the t-box expressed in t cells (t-bet) is a member of a family of t-box transcription factors that regulates the expression of ifn-gamma and plays a crucial role in th1 cell differentiation and cell-mediated immunity. we cloned and sequenced t-bet cdna for the first time from non-mammalian species, ginbuna crucian carp. ginbuna t-bet was composed of 608 predicted amino acids and showed 41.5% ... | 2008 | 17624433 |