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members of the rkd transcription factor family induce an egg cell-like gene expression program.in contrast to animals, the life cycle of higher plants alternates between a gamete-producing (gametophyte) and a spore-producing (sporophyte) generation. the female gametophyte of angiosperms consists of four distinct cell types, including two gametes, the egg and the central cell, which give rise to embryo and endosperm, respectively. based on a combined subtractive hybridization and virtual subtraction approach in wheat (triticum aestivum l.), we have isolated a class of transcription factors ...201121457369
puroindoline-a, a lipid binding protein from common wheat, spontaneously forms prolate protein micelles in solution.the self-assembly in solution of puroindoline-a (pin-a), an amphiphilic lipid binding protein from common wheat, was investigated by small angle neutron scattering, dynamic light scattering and size exclusion chromatography. pin-a was found to form monodisperse prolate ellipsoidal micelles with a major axial radius of 112 ± 4.5 å and minor axial radius of 40.4 ± 0.18 å. these protein micelles were formed by the spontaneous self-assembly of 38 pin-a molecules in solution and were stable over a wi ...201121451840
conservation tillage, optimal water and organic nutrient supply enhance soil microbial activities during wheat (triticum aestivum l.) cultivation.the field experiments were conducted on sandy loam soil at new delhi, during 2007 and 2008 to investigate the effect of conservation tillage, irrigation regimes (sub-optimal, optimal and supra-optimal water regimes), and integrated nutrient management (inm) practices on soil biological parameters in wheat cultivation. the conservation tillage soils has shown significant (p<0.05) increase in soil respiration (81.1%), soil microbial biomass carbon (smbc) (104%) and soil dehydrogenase (dh) (59.2%) ...201124031665
[characteristics of common wheat cultivars of west siberia carrying the wheat-rye 1rs.1bl translocation].using genomic in situ hybridization, among the common wheat cultivars produced in west siberia (siberian research institute of agriculture, omsk) with the involvement of the winter wheat cultivar kavkaz carrying the wheat-rye 1rs.1bl translocation we identified three cultivars with this translocation: omskaya 29, omskaya 37, and omskaya 38. the protein and crude gluten contents in the grain of these cultivars are equal to or exceed the levels observed in cultivars without the wheat-rye transloca ...201121446180
quality of bread made from ozonated wheat (triticum aestivum l.) flour.ozone gas could be used as a fumigant during grain and flour storage. experiments were conducted to determine the effects of exposure to ozone and the effects of blending ozone-treated flour with control flour on flour functionality and bread-making quality.201121445841
efficacy of heat treatment for disinfestation of concrete grain silos.field experiments were conducted in 2007 and 2008 to evaluate heat treatment for disinfestations of empty concrete elevator silos. a mobile heat treatment unit was used to introduce heat into silos to attain target conditions of 50 degrees c for at least 6 h. ventilated plastic containers with a capacity of 100 g of wheat, triticum aestivum l., held rhyzopertha dominica (f.) (coleoptera: bostrichidae) and tribolium castaneum (herbst) (coleoptera: tenebrionidae). polyvinyl chloride containers wit ...201121882711
combined effect of iron and zinc on micronutrient levels in wheat (triticum aestivum l.).a nutrient solution experiment was conducted to investigate the effect of fe and zn supply on fe, zn, cu, and mn concentrations in wheat plants. the experiment used a factorial combination of two fe levels (0 and 5 mg l(-1)) and three zn levels (0, 0.1 and 10 mg i(-1)). the supply of fe (5 mg l(-1)) and zn (0.1 mg l(-1)) increased plant dry weight and leaf chlorophyll content compared to the fe or zn deficient (0 mg 11) treatments. however, excess zn supply (10 mg l(-1)) reduced plant dry weight ...201121882661
dispersed benzoxazinone gene cluster: molecular characterization and chromosomal localization of glucosyltransferase and glucosidase genes in wheat and rye.benzoxazinones (bxs) are major defensive secondary metabolites in wheat (triticum aestivum), rye (secale cereale), and maize (zea mays). here, we identified full sets of homeologous and paralogous genes encoding bx glucosyltransferase (gt) and bx-glucoside glucosidase (glu) in hexaploid wheat (2n = 6x = 42; aabbdd). four gt loci (tagta-tagtd) were mapped on chromosomes 7a, 7b (two loci), and 7d, whereas four glu1 loci (taglu1a-taglu1d) were on chromosomes 2a, 2b (two loci), and 2d. transcript le ...201121875895
intergenic spacer length variants in old portuguese bread wheat cultivars.the intergenic spacer of the ribosomal dna is highly variable, but is location specific in the nucleolar organizer region of the chromosomes. this study provides an event of high level of polymorphism / size variation and occurrence of 14 unique phenotypes in 48 landraces of portuguese bread wheat cultivars for igs-amplified products obtained by pcr-rflp technique performed with taqi. the attendant igs polymorphism has been used to deduce affinities between landraces. some of the high molecular ...201121869468
[morphological and genetic characteristics of leaf hairiness in wheat (triticum aestivum l.) as analyzed by computer-aided phenotyping].computer-aided image processing was used to study the morphology of leaf hairiness in the wheat cultivars saratovskaya 29 and golubka, as well as the introgressed strain 102/00i of the cultivar rodina carrying the hairiness control gene introgressed from aegilops speltoides. morphological differences in leaf hairiness were detected and described in detail. the genetic control ofhairiness was studied in two cultivars (golubka and saratovskaya 29) with similar hairiness patterns. crossing these cu ...201121866865
development of a set of compensating triticum aestivum-dasypyrum villosum robertsonian translocation lines.dasypyrum villosum (l.) candargy, a wild relative of bread wheat ( triticum aestivum l.), is the source of many agronomically important genes for wheat improvement. production of compensating robertsonian translocations (crobts), consisting of d. villosum chromosome arms translocated to homoeologous wheat chromosome arms, is one of the initial steps in exploiting this variation. the crobts for d. villosum chromosomes 1v, 4v, and 6v have been reported previously. here we report attempted crobts f ...201121961939
the effect of cultivation intensity on mineral content in grain, flakes and bran of winter wheat (triticum aestivum l.)--preliminary study.the aim of the study was to assess the effect of cultivation intensity on the ca, mg, zn and fe contents in winter wheat grain, flakes and bran. the initial material for the study comprised grain, flakes and bran of two winter wheat cultivars kobiera and bogatka. these cultivars were from two strain testing stations located in lower silesia, zybiszów and tomaszów bolesławiecki in the years 2007-2008. the cultivation was conducted on two intensity levels: lower and higher the mineral content was ...201121980867
mapping qtl for grain yield, yield components, and spike features in a doubled haploid population of bread wheat.a doubled haploid (dh) population derived from a cross between the japanese cultivar 'fukuho-kumogi' and the israeli wheat line 'oligoculm' was used to map genome regions involved in the expression of grain yield, yield components, and spike features in wheat (triticum aestivum l). a total of 371 markers (rapd, ssr, rflp, aflp, and two morphological traits) were used to construct the linkage map that covered 4190 cm of wheat genome including 28 linkage groups. the results of composite interval m ...201121635161
a proteomics view on the role of drought-induced senescence and oxidative stress defense in enhanced stem reserves remobilization in wheat.drought is one of the major factors limiting the yield of wheat (triticum aestivum l.) particularly during grain filling. under terminal drought condition, remobilization of pre-stored carbohydrates in wheat stem to grain has a major contribution in yield. to determine the molecular mechanism of stem reserve utilization under drought condition, we compared stem proteome patterns of two contrasting wheat landraces (n49 and n14) under a progressive post-anthesis drought stress, during which period ...201121621021
spectroscopic analysis of diversity of arabinoxylan structures in endosperm cell walls of wheat cultivars (triticum aestivum) in the healthgrain diversity collection.fifty bread wheat (triticum aestivum l.) cultivars were selected from the healthgrain germplasm collection based on variation in their contents of total and water-extractable arabinoxylan. ft-ir spectroscopic mapping of thin transverse sections of grain showed variation in cell wall arabinoxylan composition between the cultivars, from consisting almost entirely of low-substituted arabinoxylan (e.g., t.aestivum 'claire') to almost entirely of highly substituted arabinoxylan (e.g., t.aestivum 'man ...201121615152
phosphate utilization efficiency correlates with expression of low-affinity phosphate transporters and noncoding rna, ips1, in barley.genetic variation in phosphorus (p) efficiency exists among wheat (triticum aestivum) and barley (hordeum vulgare) genotypes, but the underlying mechanisms for the variation remain elusive. high- and low-affinity phosphate (pi) pht1 transporters play an indispensable role in p acquisition and remobilization. however, little is known about genetic variation in pht1 gene expression and association with p acquisition efficiency (pae) and p utilization efficiency (pue). here, we present quantitative ...201121606317
allelic variation, alternative splicing and expression analysis of psy1 gene in hordeum chilense roem. et schult.the wild barley hordeum chilense roem. et schult. is a valuable source of genes for increasing carotenoid content in wheat. tritordeums, the amphiploids derived from durum or common wheat and h. chilense, systematically show higher values of yellow pigment colour and carotenoid content than durum wheat. phytoene synthase 1 gene (psy1) is considered a key step limiting the carotenoid biosynthesis, and the correlation of psy1 transcripts accumulation and endosperm carotenoid content has been demon ...201121603624
enrichment of a common wheat genetic map and qtl mapping for fatty acid content in grain.dart and ssr markers were used to saturate and improve a previous genetic map of rils derived from the cross chuan35050 × shannong483. the new map comprised 719 loci, 561 of which were located on specific chromosomes, giving a total map length of 4008.4 cm; the rest 158 loci were mapped to the most likely intervals. the average chromosome length was 190.9 cm and the marker density was 7.15 cm per marker interval. among the 719 loci, the majority of marker loci were darts (361); the rest included ...201121600399
effect of four types of dietary fiber on the technological quality of pasta.the development of dietary fiber-enriched foods permits to obtain products with functional properties but can cause several problems in technological quality. the aim of this study was to study the quality of pasta obtained by replacing bread wheat flour with resistant starch ii (rsii), resistant starch iv (rsiv), oat bran (ob) and inulin (in) with the purpose of improving their nutritional quality. rsii, rsiv, ob and in were substituted for a portion of bread wheat flour at levels 2.5%, 5.0%, 7 ...201121593287
insights of interaction between small and large subunits of adp-glucose pyrophosphorylase from bread wheat (triticum aestivum l.).lack of knowledge of three dimensional structures of small and large subunits of adp- glucose pyrophosphorylase (agpase) in wheat has hindered efforts to understand the binding specifities of substrate and catalytic mechanism. thus, to understand the structure activity relationship, 3d structures were built by homology modelling based on crystal structure of potato tuber adp-glucose pyrophosphorylase. selected models were refined by energy minimization and further validated by procheck and prosa ...201121572880
investigation of genetic diversity and population structure of common wheat cultivars in northern china using dart markers.in order to help establish heterotic groups of chinese northern wheat cultivars (lines), diversity arrays technology (dart) markers were used to investigate the genetic diversity and population structure of chinese common wheat (triticum aestivum l.).201121569312
comparative analysis of the n-genome in diploid and polyploid aegilops species.the genetic classification for the n-genome chromosomes has been developed on the basis of c-banding analysis on the set of triticum aestivum × aegilops uniaristata single chromosome addition lines and examination of a. uniaristata (2n = 2 × = 14, nn), aegilops ventricosa (2n = 4 × = 28, ddnn) and aegilops recta (2n = 6 × = 42, uux(n)x(n)nn) accessions carrying intergenomic translocations using fluorescence in situ hybridisation with probes for three repetitive dna sequences as well as the 5s an ...201121556954
[antipodal complex development in the embryo sac of wheat].dynamics of an antipodal complex formation in wheat (triticum aestivum l.) has been observed in detail using a reconstruction of serial semifine sections. three consecutive crucial stages have been identified in the development of the antipodal complex: (1) proliferation of initial cells, (2) growth and functional differentiation of antipodal cells, and (3) cell apoptosis. specific features of the mitotic division of antipodal cells have been characterized. it has been shown that the structure o ...201121542339
acquisition and assimilation of nitrogen as peptide-bound and d-enantiomers of amino acids by wheat.nitrogen is a key regulator of primary productivity in many terrestrial ecosystems. historically, only inorganic n (nh(4)(+) and no(3)(-)) and l-amino acids have been considered to be important to the n nutrition of terrestrial plants. however, amino acids are also present in soil as small peptides and in d-enantiomeric form. we compared the uptake and assimilation of n as free amino acid and short homopeptide in both l- and d-enantiomeric forms. sterile roots of wheat (triticum aestivum l.) pla ...201121541281
calcium and magnesium enhance arsenate rhizotoxicity and uptake in triticum aestivum.cations such as calcium (ca) and magnesium (mg) alleviate toxicities of cationic toxicants and increase those of anionic toxicants such as arsenic (as) present as arsenate under aerobic conditions. increasing evidence exists that these phenomena are related to the outer surface electrical potential (ψ 0°) of the root cell plasma membrane (pm). short-term (48-h) nutrient culture experiments with wheat (triticum aestivum l.) seedlings investigated ca and mg effects on arsenate rhizotoxicity and up ...201121538485
cadmium pollution enhanced ozone damage to winter wheat: biochemical and physiological evidences.combined effects of ozone (03) and cadmium (cd) on growth and physiology of winter wheat (triticum aestivum l. cv. jm22) were determined. wheat plants were grown without or with cd and exposed to charcoal-filtered air (< 10 ppb o3) or elevated o3 (80 +/- 5 ppb, 7 hr/day) for 20 days. results showed that 03 considerably depressed light saturated net photosynthetic rate (-20%), stomatal conductance (-33%), chlorophyll content (-33%), and total biomass (-29%) without cd. the corresponding decreases ...201121516999
molecular characterization and genomic mapping of the pathogenesis-related protein 1 (pr-1) gene family in hexaploid wheat (triticum aestivum l.).the group 1 pathogenesis-related (pr-1) proteins, known as hallmarks of defense pathways, are encoded by multigene families in plants as evidenced by the presence of 22 and 32 pr-1 genes in the finished arabidopsis and rice genomes, respectively. here, we report the initial characterization and mapping of 23 pr-1-like (tapr-1) genes in hexaploid wheat (triticum aestivum l.), which possesses one of the largest (>16,000 megabases) genomes among monocot crop plants. sequence analysis revealed that ...201121516334
extensive and heritable epigenetic remodeling and genetic stability accompany allohexaploidization of wheat.allopolyploidy has played a prominent role in organismal evolution, particularly in angiosperms. allohexaploidization is a critical step leading to the formation of common wheat as a new species, triticum aestivum, as well as for bestowing its remarkable adaptability. a recent study documented that the initial stages of wheat allohexaploidization was associated with rampant genetic and epigenetic instabilities at genomic regions flanking a retrotransposon family named veju. although this finding ...201121515577
detection of sugar accumulation and expression levels of correlative key enzymes in winter wheat (triticum aestivum) at low temperatures.carbohydrate accumulation is common in frost-resistant plants, and many enzymes participate in this process. the sugar content and expression levels of metabolic enzymes related to sugar biosynthesis in response to drops in temperature were measured in two cultivars of winter wheat (triticum aestivum) with different cold tolerances. the results indicate that the two cultivars examined, dongnongdongmai 1 and jimai 22, accumulated high levels of carbohydrate before november 4 (above 0°c), and that ...201121512254
synthesis and biological activity of n-acyl o-indolylalkyl ethanolamines.the plant-growth regulators, indole-3-carboxylic acids, were introduced into n-acyl ethanolamines, and a series of n-acyl o-indolylalkyl ethanolamines were prepared. their biological activities to regulate rape hypocotyl elongation, cucumber cotyledon expansion and common wheat coleoptile growth were tested. the results indicate that the title compounds inhibited rape hypocotyl elongation, especially the indole-3-propionic acid derivatives, whose bioactivity was better than that of indole-3-acet ...201121512235
effect of organic and conventional crop rotation, fertilization, and crop protection practices on metal contents in wheat (triticum aestivum).the effects of organic versus conventional crop management practices (crop rotation, crop protection, and fertility management strategies) on wheat yields and grain metal (al, cd, cu, ni, pb, and zn) concentrations were investigated in a long-term field trial. the interactions between crop management practices and the season that the crop was grown were investigated using univariate and redundancy analysis approaches. grain yields were highest where conventional fertility management and crop pro ...201121495704
phylogenetic analysis of c, m, n, and u genomes and their relationships with triticum and other related genomes as revealed by lmw-gs genes at glu-3 loci.phylogenetic relationships between the c, u, n, and m genomes of aegilops species and the genomes of common wheat and other related species were investigated by using three types of low-molecular-weight glutenin subunit (lmw-gs) genes at glu-3 loci. a total of 20 lmw-gs genes from aegilops and triticum species were isolated, including 11 lmw-m type and 9 lmw-i type genes. particularly, four lmw-m type and three lmw-i type subunits encoded by the genes on the c, n, and u genomes possessed an extr ...201121491971
molecular analysis of common wheat genes encoding three types of cytosolic heat shock protein 90 (hsp90): functional involvement of cytosolic hsp90s in the control of wheat seedling growth and disease resistance.heat shock protein 90 (hsp90) molecular chaperones play important roles in plant growth and responses to environmental stimuli. however, little is known about the genes encoding hsp90s in common wheat. here, we report genetic and functional analysis of the genes specifying cytosolic hsp90s in this species. three groups of homoeologous genes (tahsp90.1, tahsp90.2 and tahsp90.3), encoding three types of cytosolic hsp90, were isolated. the loci containing tahsp90.1, tahsp90.2 and tahsp90.3 genes we ...201121488877
adverse effects of chromium oxide nano-particles on seed germination and growth in triticum aestivum l.in this study, seeds of triticum aestivum l. (poaceae) were exposed to 0-100 microg/ml chromium oxide nanoparticles (cr2o3, nps) to study the phytotoxic effects on seed germination and seedling growth. it has been observed that 25-100 microg/ml cr2o3, nps inhibited the seed germination and seedling growth in concentration dependent manner. the present study suggests that release of cr2o3, nps in environment may adversely affect the wheat production.201121485872
protein and metal cluster structure of the wheat metallothionein domain γ-e(c)-1: the second part of the puzzle.metallothioneins (mts) are small cysteine-rich proteins coordinating various transition metal ions, including zn(ii), cd(ii), and cu(i). mts are ubiquitously present in all phyla, indicating a successful molecular concept for metal ion binding in all organisms. the plant mt e(c)-1 from triticum aestivum, common bread wheat, is a zn(ii)-binding protein that comprises two domains and binds up to six metal ions. the structure of the c-terminal four metal ion binding β(e) domain was recently describ ...201121437709
retention of d genome chromosomes in pentaploid wheat crosses.the transfer of genes between triticum aestivum (hexaploid bread wheat) and t. turgidum (tetraploid durum wheat) holds considerable potential for genetic improvement of both these closely related species. five different t. aestivum/t. turgidum ssp. durum crosses were investigated using diversity arrays technology (dart) markers to determine the inheritance of parental a, b and d genome material in subsequent generations derived from these crosses. the proportions of a, b and d chromosomal segmen ...201121427754
characterization of a single recessive yield trait mutant with elevated endogenous aba concentration and deformed grains, spikelets and leaves.the characterization of yield trait mutants is important for understanding the regulation of grain yield formation in staple food crops. meh0239 is a yield trait-related mutant identified from a mutant library of the common wheat cultivar wangshuibai created by ethylmethyl sulfide (ems) treatment of dry seeds. to shed some light on the nature of this mutation, it was investigated morphologically, physiologically, anatomically and genetically. the mutant plant showed obvious phenotypic difference ...201121421375
active-site architecture of benzoxazinone-glucoside β-d-glucosidases in triticeae.the β-d-glucosidases from wheat (triticum aestivum) and rye (secale cereale) hydrolyze benzoxazinone-glucose conjugates. although wheat and rye glucosidases have high sequence identity, they have different substrate preferences; the wheat enzyme favors dimboa-glc (2-o-β-d-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one) over diboa-glc (7-demethoxy-dimboa-glc), whereas the rye enzyme preference is the opposite. to investigate the mechanism of substrate binding, we analyzed crystal structu ...201121421370
high temperature during grain fill alters the morphology of protein and starch deposits in the starchy endosperm cells of developing wheat (triticum aestivum l.) grain.high temperature during grain fill reduces wheat yield and alters flour quality. starchy endosperm cell morphology was investigated in wheat ( triticum aestivum l. 'butte 86') grain produced under a 24/17 or 37/28 °c day/night regimen imposed from anthesis to maturity to identify changes in cell structure related to the functional properties of flour. the duration of grain fill decreased substantially under the 37/28 °c regimen, but, like the 24/17 °c regimen, endosperm cells in the mature grain ...201121417450
pcr-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (triticum aestivum l.).low-molecular-weight glutenin subunits (lmw-gss) are encoded by a multi-gene family and are essential for determining the quality of wheat flour products, such as bread and noodles. however, the exact role or contribution of individual lmw-gs genes to wheat quality remains unclear. this is, at least in part, due to the difficulty in characterizing complete sequences of all lmw-gs gene family members in bread wheat. to identify full-length lmw-gs genes, a polymerase chain reaction (pcr)-based met ...201121830110
intake and digestion of whole-crop barley and wheat silages by dairy heifers.the effect of maturity at harvest on the digestibility and intake of large bale silage made from whole-crop barley and wheat when fed to growing heifers was evaluated. two crops of spring barley (hordeum distichum cv. filippa and kinnan) and 1 of winter wheat (triticum aestivum cv. olevin) were harvested at the heading, milk, and dough stages of maturity. the silage was fed to 36 dairy heifers in a balanced crossover experiment with 3 periods and 9 treatments (diets based on 3 crops and 3 stages ...201121821807
differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-de.mineral nutrition during wheat grain development has large effects on wheat flour protein content and composition, which in turn affect flour quality and immunogenic potential for a commodity of great economic value. however, it has been difficult to define the precise effects of mineral nutrition on protein composition because of the complexity of the wheat flour proteome. recent improvements in the identification of flour proteins by tandem mass spectrometry (ms/ms) and the availability of a c ...201121816081
redox changes during cold acclimation affect freezing tolerance but not the vegetative/reproductive transition of the shoot apex in wheat.cold acclimation is necessary for winter wheat (triticum aestivum l.) to achieve its genetically determined maximum freezing tolerance, and cold also fulfils the vernalisation requirement. chromosome 5a is a major regulator of these traits. the aim of the present study was to discover whether changes in the half-cell redox potential of the glutathione/glutathione disulphide (gsh/gssg) and ascorbate/dehydroascorbate (aa/dha) couples induced by cold acclimation are related to freezing tolerance an ...201121815980
susceptibility of tribolium castaneum (coleoptera: tenebrionidae) life stages to flameless catalytic infrared radiation.the susceptibility of various life stages of the red flour beetle, tribolium castaneum (herbst) (coleoptera: tenebrionidae), a pest of stored wheat, triticum aestivum l., to flameless catalytic infrared radiation in the 3-7-microm range was evaluated in the laboratory. immature stages were collected from flour infested with t. castaneum adults only for 1 d. stages collected after 1 d represented eggs (collected on day 0); those collected after 7, 14, and 21 d from day 0 represented larvae in dif ...201121404874
wheat grain development is characterized by remarkable trehalose 6-phosphate accumulation pregrain filling: tissue distribution and relationship to snf1-related protein kinase1 activity.trehalose 6-phosphate (t6p) is a sugar signal that regulates metabolism, growth, and development and inhibits the central regulatory snf1-related protein kinase1 (snrk1; akin10/akin11). to better understand the mechanism in wheat (triticum aestivum) grain, we analyze t6p content and snrk1 activities. t6p levels changed 178-fold 1 to 45 d after anthesis (daa), correlating with sucrose content. t6p ranged from 78 nmol g(-1) fresh weight (fw) pregrain filling, around 100-fold higher than previously ...201121402798
starch waxiness in hexaploid wheat (triticum aestivum l.) by nir reflectance spectroscopy.wheat (triticum aestivum l.) breeding programs are currently developing varieties that are free of amylose (waxy wheat), as well as genetically intermediate (partial waxy) types. successful introduction of waxy wheat varieties into commerce is predicated on a rapid methodology at the commodity point of sale that can test for the waxy condition. near-infrared (nir) reflectance spectroscopy, one such technology, was applied to a diverse set of hard winter (hexaploid) wheat breeders' lines represen ...201121401107
technological, rheological and yield differences among egyptian wheat varieties.a field experiment was conducted during the two winter seasons of 2007/2008 and 2008/2009 to evaluate the yield and its components, milling, technological and rheological characteristics as well as the correlation between these characteristics of eight bread wheat (triticum aestivum l.) cultivars (gemmeiza 7, gemmeiza 9, gemmeiza 10, sakha 92, sakha 93, sakha 94, giza 164 and giza 168). these cultivars were chosen because they were new.201121384350
microbial activity and water-soluble trace element species in the rhizosphere of spring wheat (triticum aestivum cv. usu-perigee).the influence of microbial activity on the concentration and speciation of trace elements (tes) was assessed in a study on the bioavailability of tes for edible plants. a growth chamber experiment with spring wheat (triticum aestivum cv. usu-perigee) was conducted and the bulk (bk) and the rhizosphere (rz) soil components were collected at maturity. a characterization of the microbial activity and population was made by measuring the microbial biomass, enzymes (acid phosphatase, arylsulfatase, d ...201121359293
major genes for na+ exclusion, nax1 and nax2 (wheat hkt1;4 and hkt1;5), decrease na+ accumulation in bread wheat leaves under saline and waterlogged conditions.two major genes for na(+) exclusion in durum wheat, nax1 and nax2, that were previously identified as the na(+) transporters tmhkt1;4-a2 and tmhkt1;5-a, were transferred into bread wheat in order to increase its capacity to restrict the accumulation of na(+) in leaves. the genes were crossed from tetraploid durum wheat (triticum turgidum ssp. durum) into hexaploid bread wheat (triticum aestivum) by interspecific crossing and marker-assisted selection for hexaploid plants containing one or both g ...201121357768
synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process.doubled haploid (dh) populations are useful to scientists and breeders in both crop improvement and basic research. current methods of producing dhs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. this report describes a simple method for synthesizing dhs (syndh) especially for allopolyploid species by utilizing meiotic restitution genes. the method involves three steps: hybridization to induce recombination, interspecific hybridization to ex ...201121356528
characterisation of a new stripe rust resistance gene yr47 and its genetic association with the leaf rust resistance gene lr52.two iranian common wheat landraces aus28183 and aus28187 from the watkins collection showed high levels of seedling resistance against australian pathotypes of leaf rust and stripe rust pathogens. chi-squared analyses of rust response segregation among f(3) populations derived from crosses of aus28183 and aus28187 with a susceptible genotype aus27229 revealed monogenic inheritance of leaf rust and stripe rust resistance. as both genotypes produced similar leaf rust and stripe rust infection type ...201121344185
development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (triticum aestivum l.).low-molecular-weight glutenin subunits (lmw-gss) play an important role in determining the bread-making quality of bread wheat. however, lmw-gss display high polymorphic protein complexes encoded by multiple genes, and elucidating the complex lmw-gs gene family in bread wheat remains challenging. in the present study, using conventional polymerase chain reaction (pcr) with conserved primers and high-resolution capillary electrophoresis, we developed a new molecular marker system for identifying ...201121344181
stable chloroplast transformation of immature scutella and inflorescences in wheat (triticum aestivum l.).chloroplast transformation in wheat was achieved by bombardment of scutella from immature embryos and immature inflorescences, respectively. a wheat chloroplast site-specific expression vector, pbagnrk, was constructed by placing an expression cassette containing neomycin phosphotransferase ii (nptii) and green fluorescent protein (gfp) as selection and reporter genes, respectively, in the intergenic spacer between atpb and rbcl of wheat chloroplast genome. integration of gfp gene in the plastom ...201121343162
assessing the role of root plasma membrane and tonoplast na+/h+ exchangers in salinity tolerance in wheat: in planta quantification methods.this work investigates the role of cytosolic na+ exclusion in roots as a means of salinity tolerance in wheat, and offers in planta methods for the functional assessment of major transporters contributing to this trait. an electrophysiological protocol was developed to quantify the activity of plasma membrane na+ efflux systems in roots, using the microelectrode ion flux estimation (mife) technique. we show that active efflux of na+ from wheat root epidermal cells is mediated by a sos1-like homo ...201121342209
lead phytotoxicity on wheat (triticum aestivum l.) seed germination and seedlings growth.lead (pb) is an environmental pollutant extremely toxic to plants and other living organisms including humans. to assess pb phytotoxicity, experiments focusing on germination of wheat seeds were germinated in a solution containing pb (no(3))(2) (0.05; 0.1; 0.5; 1g/l) during 6 days. lead accumulation in seedlings was positively correlated with the external concentrations, and negatively correlated with morphological parameters of plant growth. lead increased lipid peroxidation, enhanced soluble p ...201121333942
physical and stress-strain properties of wheat (triticum aestivum) kernel.two hard wheat varieties and one soft variety grown under the same agroecological conditions were analyzed for their physical and uniaxial stress-strain compression properties.201121328363
tanf-yb3 is involved in the regulation of photosynthesis genes in triticum aestivum.nuclear factor y (nf-y) transcription factor is a heterotrimer comprised of three subunits: nf-ya, nf-yb and nf-yc. each of the three subunits in plants is encoded by multiple genes with differential expression profiles, implying the functional specialisation of nf-y subunit members in plants. in this study, we investigated the roles of nf-yb members in the light-mediated regulation of photosynthesis genes. we identified two nf-yb members from triticum aestivum (tanf-yb3 & 7) which were markedly ...201121327447
the influence of temperature on plant development in a vernalization-requiring winter wheat: a 2-de based proteomic investigation.in this work, proteomics was used to study the influence of both optimal and low temperatures on growth and development in a vernalization-requiring winter wheat (triticum aestivum l. cv cheyenne) after prolonged times of treatment. for this purpose, plants were grown at optimal temperature (20°c) for 14 days (zero point) after which half were transferred to conditioned chambers kept at 4°c for a period of 63 days. cold tolerance, as estimated from lethal temperatures (lt(50)), and phenological ...201121320650
bac libraries from wheat chromosome 7d: efficient tool for positional cloning of aphid resistance genes.positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. bac libraries represent an essential tool for positional cloning. however, wheat bac libraries comprise more than million clones, which makes their screening very laborious. here, we present a targeted approach based on chromosome-specific bac libraries. such libraries were constructed from flow-sorted arms of wheat chromosome 7d. a library from the short arm (7ds) consisting of 49,152 clones ...201121318113
detecting ingested plant dna in soil-living insect larvae.although a significant proportion of plant tissue is located in roots and other below-ground parts of plants, little is known on the dietary choices of root-feeding insects. this is caused by a lack of adequate methodology which would allow tracking below-ground trophic interactions between insects and plants. here, we present a dna-based approach to examine this relationship. feeding experiments were established where either wheat (triticum aestivum) or maize (zea mays) was fed to agriotes larv ...201121317975
evolution of polyploid triticum wheats under cultivation: the role of domestication, natural hybridization and allopolyploid speciation in their diversification.the evolution of the polyploid triticum wheats is distinctive in that domestication, natural hybridization and allopolyploid speciation have all had significant impacts on their diversification. in this review, i outline the phylogenetic relationships of cultivated wheats and their wild relatives and provide an overview of the recent progress and remaining issues in understanding the genetic and ecological factors that favored their evolution. an attempt is made to view the evolution of the poly ...201121317146
thiol redox-sensitive seed proteome in dormant and non-dormant hybrid genotypes of wheat.the thiol redox-sensitive and the total proteome in harvest-ripe grains of closely related genotypes of wheat (triticum aestivum l.), with either a dormant or a non-dormant phenotype, were investigated using hybrid lines of spring wheat double haploid population segregating transgressively, to gain further insight into seed dormancy controlling events. redox signalling by reactive oxygen species has been shown to play a role in seed dormancy alleviation. thiol-disulfide proteins are of particula ...201121295800
three-dimensional distribution of vessels, passage cells and lateral roots along the root axis of winter wheat (triticum aestivum).the capacity of a plant to absorb and transport water and nutrients depends on anatomical structures within the roots and their co-ordination. however, most descriptions of root anatomical structure are limited to 2-d cross-sections, providing little information on 3-d spatial relationships and hardly anything on their temporal evolution. three-dimensional reconstruction and visualization of root anatomical structures can illustrate spatial co-ordination among cells and tissues and provide new i ...201121289027
accurate inference of shoot biomass from high-throughput images of cereal plants.with the establishment of advanced technology facilities for high throughput plant phenotyping, the problem of estimating plant biomass of individual plants from their two dimensional images is becoming increasingly important. the approach predominantly cited in literature is to estimate the biomass of a plant as a linear function of the projected shoot area of plants in the images. however, the estimation error from this model, which is solely a function of projected shoot area, is large, prohi ...201121284859
proteomic analysis of peripheral layers during wheat (triticum aestivum l.) grain development.grains of hexaploid wheat, triticum aestivum (cv. récital), were collected at 15 stages of development, from anthesis to physiological maturity, 0-700°c days (degree days after anthesis). two hundred and seven proteins of grain peripheral layers (inner pericarp, hyaline, testa and aleurone layer) were identified by 2-de, maldi-tof ms and data mining, then were classified in 16 different functional categories. study of the protein expression over time allowed identification of five main profiles ...201121268267
contrasting cdna-aflp profiles between crown and leaf tissues of cold-acclimated wheat plants indicate differing regulatory circuitries for low temperature tolerance.low-temperature (lt) tolerance in winter wheat (triticum aestivum l.) is an economically important but complex trait. four selected wheat genotypes, a winter hardy cultivar, norstar, a tender spring cultivar, manitou and two near-isogenic lines with vrn-a1 (spring norstar) and vrn-a1 (winter manitou) alleles of manitou and norstar were cold-acclimated at 6°c and crown and leaf tissues were collected at 0, 2, 14, 21, 35, 42, 56 and 70 days of cold acclimation. cdna-aflp profiling was used to dete ...201121267634
quantitative trait loci analysis for resistance to cephalosporium stripe, a vascular wilt disease of wheat.cephalosporium stripe, caused by cephalosporium gramineum, can cause severe loss of wheat (triticum aestivum l.) yield and grain quality and can be an important factor limiting adoption of conservation tillage practices. selecting for resistance to cephalosporium stripe is problematic; however, as optimum conditions for disease do not occur annually under natural conditions, inoculum levels can be spatially heterogeneous, and little is known about the inheritance of resistance. a population of 2 ...201121258997
proteome analysis of wheat leaf under salt stress by two-dimensional difference gel electrophoresis (2d-dige).salt stress is a major abiotic stress that limits agricultural productivity in many regions of the world. to understand the molecular basis of the salt stress response in wheat (triticum aestivum l.), a proteomic approach was used to identify the salt stress-responsive proteins in an elite chinese wheat cultivar, zhengmai 9023, which exhibits a high yield, superior gluten quality and better biotic resistance. three-week-old seedlings were treated with nacl of four different concentrations (1.0%, ...201121257186
[analysis of common wheat varieties and near-isogenic lines by pcr with allele-specific primers for gli-1 and glu-3 loci].the allelic characteristics of the gli-a1, gli-b1, gli-d1 and glu-a3 loci of 14 bread wheat varieties and 6 near-isogenic lines derived from bezostaya 1 have been detected by pcr analysis. the conformity of molecular-genetic data and electrophoresis of storage proteins has been determined: the allelic variants of gliadins gli-a1o and gi-a1m correspond to the pcr-allele glia1.2, the gliadin variants gli-a1f, gli-a1b, gli-a1c correspond to the pcr-allele glia 1.1, the allelic variants gli-b1b, gli ...201121254616
aphid-parasitoid community structure on genetically modified wheat.since the introduction of genetically modified (gm) plants, one of the main concerns has been their potential effect on non-target insects. many studies have looked at gm plant effects on single non-target herbivore species or on simple herbivore-natural enemy food chains. agro-ecosystems, however, are characterized by numerous insect species which are involved in complex interactions, forming food webs. in this study, we looked at transgenic disease-resistant wheat (triticum aestivum) and its e ...201121247941
embryo and endosperm development in wheat (triticum aestivum l.) kernels subjected to drought stress.the aim of the present work was to reveal the histological alterations triggered in developing wheat kernels by soil drought stress during early seed development resulting in yield losses at harvest. for this purpose, observations were made on the effect of drought stress, applied in a controlled environment from the 5th to the 9th day after pollination, on the kernel morphology, starch content and grain yield of the drought-sensitive cappelle desprez and drought-tolerant plainsman v winter whea ...201121246199
molecular evolution of two duplicated cdpk genes cpk7 and cpk12 in grass species: a case study in wheat (triticum aestivum l.).gene duplication contributes to the expansion of gene families and subsequent functional diversification. calcium-dependent protein kinases (cdpks) are members of an important calcium sensor family involved in abiotic and biotic stress signaling in plants. we report here the molecular evolution and expression analysis of a pair of duplicated cdpk genes cpk7 and cpk12 that arose in the common ancestor of grass species. with higher nonsynonymous/synonymous ratios (dn/ds, or ω), cpk12 genes appear ...201121241786
identification of changes in wheat (triticum aestivum l.) seeds proteome in response to anti-trx s gene.thioredoxin h (trx h) is closely related to germination of cereal seeds. the cdna sequences of the thioredoxin s (trx s) gene from phalaris coerulescens and the thioredoxin h (trx h) gene from wheat are highly homologous, and their expression products have similar biological functions. transgenic wheat had been formed after the antisense trx s was transferred into wheat, and it had been certified that the expression of trx h decreased in transgenic wheat, and transgenic wheat has high resistance ...201121811579
chromosomal and genome-wide molecular changes associated with initial stages of allohexaploidization in wheat can be transit and incidental.genomic instability can be induced by nascent allopolyploidization in plants. however, most previous studies have not defined to what extent the allopolyploidy-induced rapid genomic instability represents a general response, and hence important to evolution, or merely incidental events occurring stochastically in a limited number of individuals. we report here that in a newly formed allohexaploid wheat line between tetraploid wheat triticum turgidum subsp. durum (genome bbaa) and aegilops tausch ...201121797821
factors responsible for deep-sowing tolerance in wheat seedlings: varietal differences in cell proliferation and the co-ordinated synchronization of epidermal cell expansion and cortical cell division for the gibberellin-mediated elongation of first internodes.a wheat cultivar, triticum aestivum 'hong mang mai', shows tolerance to deep-sowing conditions by extreme elongation of the first internode, likely mediated by the gibberellin (ga) response. to understand factors involved in the response of this deep-sowing-tolerant cultivar, cell expansion and division that confer elongation on the first internodes of wheat seedlings were investigated.201121791455
[the role of chromosomes from homeologous group 5 in regulation of grain hardness and protein content in substitution lines of common wheat cultivar saratovskaya 29].genetic regulation of grain hardness and protein content in intervarietal substitution lines for chromosomes of homeologous group 5 was examined. common wheat cultivar saratovskaya 29 with high bread-backing properties served as the recipient. donors of chromosomes 5a and 5d were 18 cultivars with variable traits examined, including high-protein cultivars (atlas 66 and diamant 2), and soft-grain cultivars (ul'yanovka and chinese spring). analysis of substitution lines pointed to a substantial ef ...201121786668
[ecotoxicity of synthetical musks on wheat (triticum aestivum) based on seed germination].with soil culture the ecotoxicological effects of galaxolide (hhcb) and tonalide (ahtn) on the germination of wheat (triticum aestivum) seeds were investigated. and the influence of these two synthetical musks on the wheat germination rate, inhibition rate of root elongation and inhibition rate of shoot elongation were studied. the results showed that there were no obvious dose-effect relationship between wheat germination rate and concentrations of these two synthetical musks. lower dose (hhcb ...201121780608
use of methylation filtration and c(0)t fractionation for analysis of genome composition and comparative genomics in bread wheat.we investigated the compositional and structural differences in sequences derived from different fractions of wheat genomic dna obtained using methylation filtration and c(0)t fractionation. comparative analysis of these sequences revealed large compositional and structural variations in terms of gc content, different structural elements including repeat sequences (e.g., transposable elements and simple sequence repeats), protein coding genes, and non-coding rna genes. a correlation between meth ...201121777856
deciphering the genetics of flowering time by an association study on candidate genes in bread wheat (triticum aestivum l.).earliness is very important for the adaptation of wheat to environmental conditions and the achievement of high grain yield. a detailed knowledge of key genetic components of the life cycle would enable an easier control by the breeders. the objective of the study was to investigate the effect of candidate genes on flowering time. using a collection of hexaploid wheat composed of 235 lines from diverse geographical origins, we conducted an association study for six candidate genes for flowering ...201121761163
pattern of solutes accumulated during leaf osmotic adjustment as related to duration of water deficit for wheat at the reproductive stage.this study examined expression of osmotic adjustment (oa) and accumulation of solutes in wheat (triticum aestivum l.) leaves in response to water deficit (wd) imposed at the reproductive stage. two contrasting cultivars, hartog and sunco (putatively high and low in oa capacity, respectively), were grown in deep (viz. 80 cm) pots in a controlled environment. in a sandy substrate, leaf oa was 5-times greater in hartog compared with sunco. at 21 d of wd treatment, k(+) only accounted for 12% of oa ...201121741263
modeling and phylogeny analysis of bread wheat mnsod.superoxide dismutase (sod) acts as first line of defense against oxidative and genetic stress. manganese superoxide dismutase (mnsod), found in mitochondria or peroxisomes, contains mn(iii) at the active site. therefore, it is of interest to study mnsod from bread wheat (a grain crop). however, a structure model is not yet solved for bread wheat mnsod. hence, we describe the structure model of bread wheat mnsod developed using homology model. the model provides molecular insight to metal binding ...201121738317
inhibition by wheat sprout (triticum aestivum) juice of bisphenol a-induced oxidative stress in young women.for health of future generation, fertile young women should be monitored for exposure of endocrine disrupting chemicals (edcs). among edcs, bisphenol a (bpa) is suggested to induce reactive oxygen species (ros) which play an important role in pathologies of female diseases such as endometriosis. on the other hand, previous studies suggested that sprouts of wheat (triticum aestivum) have antimutagenicity and antioxidant activity. we performed the 2 weeks intervention of wheat sprout juice (100ml/ ...201121736952
effects of sterol-binding agent nystatin on wheat roots: the changes in membrane permeability, sterols and glycoceramides.plant sterols are important multifunctional lipids, which are involved in determining membrane properties. biophysical characteristics of model lipid and isolated animal membranes with altered sterol component have been intensively studied. in plants however, the precise mechanisms of involvement of sterols in membrane functioning remain unclear. in present work the possible interactions between sterols and other membrane lipids in plant cells were studied. a useful experimental approach for elu ...201121726881
heterotrimeric gα subunit from wheat (triticum aestivum), ga3, interacts with the calcium-binding protein, clo3, and the phosphoinositide-specific phospholipase c, pi-plc1.the canonical gα subunit of the heterotrimeric g protein complex from wheat (triticum aestivum), ga3, and the calcium-binding protein, clo3, were revealed to interact both in vivo and in vitro and clo3 was shown to enhance the gtpase activity of ga3. clo3 is a member of the caleosin gene family in wheat with a single ef-hand domain and is induced during cold acclimation. bimolecular fluorescent complementation (bifc) was used to localize the interaction between clo3 and ga3 to the plasma membran ...201121725861
field controlled experiments of mercury accumulation in crops from air and soil.field open top chambers (otcs) and soil mercury (hg) enriched experiments were employed to study the influence of hg concentrations in air and soil on the hg accumulation in the organs of maize (zea mays l.) and wheat (triticum aestivum l.). results showed that hg concentrations in foliages were correlated significantly (p < 0.05) with air hg concentrations but insignificantly correlated with soil hg concentrations, indicating that hg in crop foliages was mainly from air. hg concentrations in ro ...201121723013
identification of differentially expressed genes in three-pistil mutation in wheat using annealing control primer system.the common wheat line three-pistil (tp) is a valuable mutant for wheat breeding. the tp mutation has normal spike morphology; however, it only produces three pistils per floret. therefore, it has potential to increase the grain number per spike. in order to determine the underlying molecular mechanism, an annealing control primer system was used to identify the different expressed genes in three-pistil mutation. using 120 arbitrary acp primers, we identified three differentially expressed genes ...201121722717
assessment of genetic diversity of wheat genotypes by resistance gene analog-est markers.resistance gene analog-expressed sequence tag (rga-est)-based markers have been used for variety discrimination and studies of genetic diversity in wheat. our aim is to increase the competitiveness of public wheat breeding programs through intensive use of modern selection technologies, mainly marker-assisted selection. the genetic diversity of 77 wheat nucleotide binding site (nbs)-containing rga-ests was assessed. resistant and susceptible bread wheat (triticum aestivum) genotypes were used as ...201121710462
distribution of gluten proteins in bread wheat (triticum aestivum) grain.gluten proteins are the major storage protein fraction in the mature wheat grain. they are restricted to the starchy endosperm, which forms white flour on milling, and interact during grain development to form large polymers which form a continuous proteinaceous network when flour is mixed with water to give dough. this network confers viscosity and elasticity to the dough, enabling the production of leavened products. the starchy endosperm is not a homogeneous tissue and quantitative and qualit ...201121693664
nema, a functional-structural model of nitrogen economy within wheat culms after flowering. i. model description.models simulating nitrogen use by plants are potentially efficient tools to optimize the use of fertilizers in agriculture. most crop models assume that a target nitrogen concentration can be defined for plant tissues and formalize a demand for nitrogen, depending on the difference between the target and actual nitrogen concentrations. however, the teleonomic nature of the approach has been criticized. this paper proposes a mechanistic model of nitrogen economy, nema (nitrogen economy model with ...201121685431
nema, a functional-structural model of nitrogen economy within wheat culms after flowering. ii. evaluation and sensitivity analysis.simulating nitrogen economy in crop plants requires formalizing the interactions between soil nitrogen availability, root nitrogen acquisition, distribution between vegetative organs and remobilization towards grains. this study evaluates and analyses the functional-structural and mechanistic model of nitrogen economy, nema (nitrogen economy model within plant architecture), developed for winter wheat (triticum aestivum) after flowering.201121685429
profiles of phenolic compounds in modern and old common wheat varieties determined by liquid chromatography coupled with time-of-flight mass spectrometry.the health-promoting properties of common wheat (triticum aestivum l.) have been largely attributed to the presence of unique phytochemicals of whole grains. the aim of this study was to profile the phenolic content of 16 old and 6 modern italian wheat varieties, cropped in the same location and growing season. high variability was observed among the investigated wheat genotypes, both in the free and bound phenolic extracts. the total polyphenol content ranged from 885.5 to 1715.9 μmol gae/100 g ...201121683368
molecular cloning and variation of ω-gliadin genes from a somatic hybrid introgression line ii-12 and parents (triticum aestivum cv. jinan 177 and agropyron elongatum). 201121677400
physical localization of nors and its length variants in old portuguese durum wheat cultivars.the variation at the internal transcribed spacer (its) region of the ribosomal dna has been correlated with the number of nucleolar organizer regions (nors) in some plant species. besides, the number of nors might influence the rate of homogenization of the rdna repeats. in recent studies, its length variants were detected in bread wheat cultivars but no reports about their presence in durum wheat were found. in the present study, we localized and identified the nors of 51 old portuguese durum w ...201121677393
[effects of nitrogen application and elevated atmospheric co2 on electron transport and energy partitioning in flag leaf photosynthesis of wheat].wheat (triticum aestivum) plants were pot-cultured in open top chambers at the nitrogen application rate of 0 and 200 mg x kg(-1) soil and the atmospheric co2 concentration of 400 and 760 micromol x mol(-1). through the determination of flag leaf nitrogen and chlorophyll contents, photosynthetic rate (pn)-intercellar co2 concentration (ci) response curve, and chlorophyll fluorescence parameters at heading stage, the photosynthetic electron transport rate and others were calculated, aimed to inve ...201121657023
association mapping of dynamic developmental plant height in common wheat.drought as a major abiotic stress often occurs from stem elongation to the grain filling stage of wheat in northern china. plant height (ph) is a suitable trait to model the dissection of drought tolerance. the purposes of the present study were to validate molecular markers for ph developmental behavior and identify elite alleles of molecular markers. after the phenotyping of 154 accessions for ph dynamic development under well-watered (ww) and drought stressed (ds) conditions, and the genotypi ...201121647605
autoimmune response and repression of mitotic cell division occur in inter-specific crosses between tetraploid wheat and aegilops tauschii coss. that show low temperature-induced hybrid necrosis.common wheat is an allohexaploid species originating from a naturally occurring inter-specific cross between tetraploid wheat and the diploid wild wheat aegilops tauschii coss. artificial allopolyploidization can produce synthetic hexaploid wheat. however, synthetic triploid hybrids show four types of hybrid growth abnormalities: type ii and iii hybrid necrosis, hybrid chlorosis, and severe growth abortion. of these hybrid abnormalities, type ii necrosis is induced by low temperature. under low ...201121645146
the effect of extremely diluted agitated gibberellic acid (10e-30) on wheat stalk growth--a two researcher pilot study.use of a wheat growth bio assay after 7 days in research on homeopathic dilutions of gibberellic acid.201121641523
automated flow-based anion-exchange method for high-throughput isolation and real-time monitoring of rubisco in plant extracts.in this work, a miniaturized, completely enclosed multisyringe-flow system is proposed for high-throughput purification of rubisco from triticum aestivum extracts. the automated method capitalizes on the uptake of the target protein at 4°c onto q-sepharose fast flow strong anion-exchanger packed in a cylindrical microcolumn (105 × 4 mm) followed by a stepwise ionic-strength gradient elution (0-0.8 mol/l nacl) to eliminate concomitant extract components and retrieve highly purified rubisco. the m ...201121641435
dynamic management of crop diversity: from an experimental approach to on-farm conservation.in agricultural systems, biodiversity includes diversity within species and among species and provides many benefits for production, resilience and conservation. this article addresses the effects of a strategy of in situ conservation called dynamic management (dm) on population evolution, adaptation and diversity. two french dm initiatives are considered, the first one corresponding to an experimental context, the second to an on-farm management. results from a study over 26 years of experiment ...201121640955
genetic diversity analysis of abiotic stress response gene tasnrk2.7-a in common wheat.sucrose non-fermenting1-related protein kinase 2 (snrk2) plays a key role in plant stress signaling transduction pathways. in this study, one copy of tasnrk2.7, a snrk2 member of common wheat, was isolated and characterized for nucleotide diversity among 45 wheat accessions with different stress-response features. most of the accessions were elite wheat cultivars, which had been subject to population bottlenecks and intensive selection during breeding. nucleotide and haplotype diversity across t ...201121637995
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