Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| evaluation of the bactericidal effect of nd:yag, er:yag, er,cr:ysgg laser radiation, and antimicrobial photodynamic therapy (apdt) in experimentally infected root canals. | in recent years, various laser systems have been introduced into the field of laser-assisted endodontic therapy. the aim of this study was to evaluate the bactericidal effect of nd:yag, er:yag, er,cr:ysgg laser radiation, and antimicrobial photodynamic therapy (apdt) in experimentally infected root canals compared with standard endodontic treatment of 5.25% sodium hypochlorite (naclo) irrigation. | 2012 | 23168798 |
| enterococcus and streptococcus spp. associated with chronic and self-medicated urinary tract infections in vietnam. | urinary tract infections (uti) are one of the most common infections among women worldwide. e. coli often causes more than 75% of acute uncomplicated uti, however, little is known about how recurrent utis and indiscriminate use of antimicrobials affect the aetiology of utis. this study aimed to establish the aetiology of uti in a population of recurrent and self-medicated patients referred from pharmacies to a hospital in hanoi, vietnam and to describe genotypes and antimicrobial susceptibility ... | 2012 | 23176582 |
| recurrent endophthalmitis after trabeculectomy for glaucoma: importance of timely filtering bleb coverage for the conjunctiva. | to report the occurrence of endophthalmitis after trabeculectomy for glaucoma that was treated by vitrectomy without bleb closure and recurred 3 times after an initial clinically inapparent period of about 2 years. | 2012 | 23185177 |
| persistence of bacteria like pseudomonas aeruginosa in non-healing venous ulcers. | there is evidence that chronic inflammation due to bacterial wound colonization delays wound healing. | 2012 | 23220150 |
| antibiotic resistance genes and virulence factors in enterococcus faecium and enterococcus faecalis from diseased farm animals: pigs, cattle and poultry. | eighty enterococcal isolates (e. faecium, n = 38, e. faecalis, n = 42) from diseased farm animals (swine, cattle, poultry) in lithuania have been studied for the prevalence of antibiotic resistance and for resistance and virulence genetic determinants. 86% of e. faecium and 71% of e. faecalis isolates were multidrug resistant (resistant to three or more unrelated antibiotics). resistance to aminoglycosides, tetracycline and erythromycin was found most frequently in both species (61%, 69%) and wa ... | 2012 | 23214361 |
| qualitative comparison of sonic or laser energisation of 4% sodium hypochlorite on an enterococcus faecalis biofilm grown in vitro. | the effectiveness of sonic activation, laser activation and syringe irrigation of 4% sodium hypochlorite in removing an enterococcus faecalis biofilm was compared. biofilms were grown in extracted human single rooted teeth using a flow cell apparatus. after 4 weeks' growth, teeth were subjected to each treatment using 4% sodium hypochlorite and radicular dentinal surfaces of the root canals were analysed by scanning electron microscopy. results showed that sonic activation and syringe irrigation ... | 2012 | 23211068 |
| [emergence of linezolid-resistant enterococcus faecalis strains from two inpatients in a pediatric ward]. | we report herein on the isolation of three linezolid-resistant enterococcus faecalis strains in 2011 from two pediatric inpatients at kitasato university hospital, japan. three linezolid resistant strains were isolated from two patients who shared the same room of a pediatric inpatient ward. two linezolid resistant strains were isolated from patient a who had been treated with a total of 17,600mg of linezolid during 60 days of hospitalization (strains 1 and 2). the linezolid resistant e. faecali ... | 2012 | 23198574 |
| frequency of ace, epa and elra genes in clinical and environmental strains of enterococcus faecalis. | surface proteins play an important role in the pathogenesis of enterococcal infections. some of them are candidates for a vaccine, e.g., the frequency of endocarditis in rats vaccinated with ace protein was 75 % as 12 opposed to 100 % in those who weren't. however, there are other components of enterococcal cells, such as epa antigens or internalin-like proteins, which may be used in the prophylaxis of infections caused by them. however, also other virulence factors and resistance to antibiotics ... | 2012 | 24293719 |
| persistence and growth of the fecal indicator bacteria enterococci in detritus and natural estuarine plankton communities. | enterococci are used to evaluate recreational-water quality and health risks in marine environments. in addition to their occurrence in feces of warm blooded animals, they are also common epiphytes. we investigated the contribution of plankton- or particle-associated enterococci in estuarine and coastal water. seven water and size-fractionated plankton samples were collected monthly between april 2008 and january 2009 in the tidal reaches of the skidaway river (georgia, usa). each size fraction, ... | 2012 | 22327586 |
| isolation and identification of lactic acid bacteria from fermented red dragon fruit juices. | red dragon fruit or red pitaya is rich in potassium, fiber, and antioxidants. its nutritional properties and unique flesh color have made it an attractive raw material of various types of food products and beverages including fermented beverages or enzyme drinks. in this study, phenotypic and genotypic methods were used to confirm the identity of lactic acid bacteria (lab) appeared in fermented red dragon fruit (hylocereus polyrhizus) beverages. a total of 21 isolates of lab were isolated and ch ... | 2012 | 22924854 |
| fatal enterococcus durans aortic valve endocarditis: a case report and review of the literature. | most enterococcal endocarditis is caused by enterococcus faecalis and enterococcus faecium. enterococcus durans is a rare member of non-faecalis, non-faecium enterococcal species and is found in the intestines of animals. e durans endocarditis is a very rare infection-only two cases of endocarditis in humans have been reported in the literature-and usually associated with good outcomes when treated with appropriate antibiotics. we report the first case of fatal e durans endocarditis. this patien ... | 2012 | 22684831 |
| prevalence and characterization of antibiotic resistant enterococcus faecalis in french cheeses. | prevalence of enterococci and antibiotic resistance profiles of enterococcus faecalis was analyzed in 126 french cheeses from retail stores. forty-four percent of pasteurized or thermised-milk cheeses, and up to 92% of raw-milk cheeses contained detectable enterococci. a total of 337 antibiotic resistant enterococci were isolated in 29% and 60% of pasteurized-milk and raw-milk cheeses, respectively. e. faecalis was the predominant antibiotic resistant species recovered (81%), followed by enteroc ... | 2012 | 22608223 |
| eradication of enterococci biofilms by lactic acid alone and combined with chlorhexidine and cetrimide. | the antimicrobial activity of lactic acid (la) alone or in combination with chlorhexidine (chx) and cetrimide (ctr) against three enterococcus faecalis strains, e. faecalis atcc 29212, e. faecalis ef-d1 and e. faecalis u-1765, one enterococcus durans strain and one dual-species biofilm was investigated. | 2012 | 22549691 |
| molecular analysis of the bacteriocin-encoding plasmid pdgl1 from enterococcus durans and genetic characterization of the durancin gl locus. | enterococci constitute a significant component of the lactic acid bacteria normally present in the intestinal microflora and include strains that produce bacteriocins. the genetic determinants for durancin gl in enterococcus durans 41d were identified on the 8347 bp plasmid pdgl1 by plasmid curing experiments. pdgl1 contained nine putative orfs, with orf1 and orf2 encoding plasmid replication proteins, and orf3 and orf6 showing high similarity to genes encoding mobilization proteins. the predict ... | 2012 | 22493306 |
| heat resistance of thermoduric enterococci isolated from milk. | enterococci are reported to survive pasteurisation but the extent of their survival is unclear. sixty-one thermoduric enterococci isolates were selected from laboratory pasteurised milk obtained from silos in six dairy factories. the isolates were screened to determine log(10) reductions incurred after pasteurisation (63°c/30 min) and ranked from highest to lowest log(10) reduction. two isolates each of enterococcus faecalis, enterococcus faecium, enterococcus durans and enterococcus hirae, exhi ... | 2012 | 22260926 |
| evaluation of species-specific pcr, bruker ms, vitek ms and the vitek 2 system for the identification of clinical enterococcus isolates. | the purpose of this investigation was to compare the performance of species-specific polymerase chain reaction (pcr), matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof ms) and phenotypic identification systems for the identification of enterococcus species. a total of 132 clinical isolates were investigated by the following: (1) a multiplex real-time pcr assay targeting ddl enterococcus faecium, ddl enterococcus faecalis, vanc1 and vanc2/c3 genes, and a high ... | 2012 | 22706514 |
| characterization of the antibacterial activity and the chemical components of the volatile oil of the leaves of rubus parvifolius l. | rubus parvifolius l. (rp) is a medicinal herb that possesses antibacterial activity. in this study, we extracted the volatile oil from the leaves of rp to assess its antibacterial activity and analyze its chemical composition. a uniform distribution design was used to optimize the extraction procedure, which yielded 0.36% (w/w) of light yellowish oil from the water extract of rp leaves. we found that the extracted oil effectively inhibited the growth of a wide range of gram positive and negative ... | 2012 | 22732887 |
| the variability of bacterial aerosol in poultry houses depending on selected factors. | this study is aimed at evaluation of bacterial air contamination in intensive poultry breeding. the evaluation was based on the determined levels of bacterial concentrations and qualitative identification of isolated microorganisms. | 2012 | 22729495 |
| antibacterial and antimycobacterial lignans and flavonoids from larrea tridentata. | three lignans and four flavonoids were isolated and characterized from larrea tridentata and compounds were tested against 16 bacterial species/strains. results showed that: dihydroguaiaretic acid (1) had activity towards methicillin resistant (mr) staphylococcus aureus (minimum inhibitory concentration (mic) 50 µg/ml) and multidrug-resistant (mdr) strains of mycobacterium tuberculosis (mic 12.5-50 µg/ml); 4-epi-larreatricin (2) was active against enterobacter cloacae (mic 12.5 µg/ml), as well a ... | 2012 | 22422605 |
| kinetics and morphology of polymicrobial biofilm formation on polypropylene mesh. | we examined the ability of three clinical bacterial isolates to form mixed biofilms on surgical polypropylene mesh (ppm) in vitro. the three strains--staphylococcus aureus, enterococcus faecalis, and enterobacter cloacae--were isolated from a patient with an infected ppm. staphylococcus aureus and e. faecalis (alone and in combination) were inoculated into culture containing squares of ppm and allowed to attach and propagate into mature biofilms. enterococcus faecalis initially attached to the m ... | 2012 | 22364207 |
| microbial diversity in failed endodontic root-filled teeth. | persistent/secondary infections of human root canals play an important role in the failure of endodontic treatment. this study used 16s rrna sequencing to assess microbial diversity in root-filled teeth associated with failed endodontic treatment. | 2012 | 22613548 |
| microbial spectrum and resistance patterns in endophthalmitis: a 21-year (1988-2008) review in northeast united states. | to study the microbial spectrum and antibacterial susceptibility of vitreous cultures in a tertiary referral center in northeast united states. | 2012 | 22506856 |
| antifungal activity and composition of essential oils of conyza canadensis herbs and roots. | essential oils from herbs and roots of conyza canadensis (horseweed), collected in hungary, were obtained by hydrodistillation. the chemical compositions of the oils were analysed by combination of gc and gc/ms. the major constituent of the oil obtained from the aerial parts of horseweed was limonene (78%), while the main component of root oil was 2z,8z-matricaria ester. the antimicrobial activities of the oils were tested on gram-positive (enterococcus faecalis, staphylococcus aureus, and strep ... | 2012 | 23049473 |
| reversible monensin adaptation in enterococcus faecium, enterococcus faecalis and clostridium perfringens of cattle origin: potential impact on human food safety. | to determine the stability/reversibility and mechanism of monensin adaptation in monensin-treated cattle isolates compared with reference bacterial isolates, exposed in vitro to high monensin concentrations. | 2012 | 22740589 |
| antimicrobial activity of filamentous fungi isolated from highly antibiotic-contaminated river sediment. | filamentous fungi are well known for their production of substances with antimicrobial activities, several of which have formed the basis for the development of new clinically important antimicrobial agents. recently, environments polluted with extraordinarily high levels of antibiotics have been documented, leading to strong selection pressure on local sentinel bacterial communities. in such microbial ecosystems, where multidrug-resistant bacteria are likely to thrive, it is possible that certa ... | 2012 | 22957125 |
| possible ecological role of pseudopterosins g and p-u and seco-pseudopterosins j and k from the gorgonian pseudopterogorgia elisabethae from providencia island (sw caribbean) in regulating microbial surface communities. | the gorgonian pseudopterogorgia elisabethae collected at providencia island (colombia) has an unfouled surface, free of obvious algal and invertebrate growth. this gorgonian produces significant amounts of the glycosilated diterpenes pseudopterosins and seco-pseudopterosins (ps and seco-ps). our previous experiments have shown activity of these compounds against eukaryotic (human cancer cell lines and candida albicans) and prokaryotic cells (staphylococcus aureus and enterococcus faecalis). howe ... | 2012 | 22932867 |
| antimicrobial effect of conventional root canal medicaments vs propolis against enterococcus faecalis, staphylococcus aureus and candida albicans. | to evaluate and compare antimicrobial effect of various root canal medicaments against enterococcus faecalis, staphylococcus aureus and candida albicans. | 2012 | 22918001 |
| in vitro antimicrobial activity of ah plus, endorez and epiphany against microorganisms. | the aim of the present study was to evaluate the antimicrobial activity of endodontic sealers against microorganisms. | 2012 | 23257479 |
| the effects of different concentrations of chlorhexidine gluconate on the antimicrobial properties of mineral trioxide aggregate and calcium enrich mixture. | the aim of this study was to evaluate the antimicrobial activity of mineral trioxide aggregate (mta) and calcium enrich mixture) cem (mixed with different concentrations of chlorhexidine (chx). | 2012 | 23162590 |
| comparative study of the antimicrobial effect of three irrigant solutions (chlorhexidine, sodium hypochlorite and chlorhexidinated mums). | to compare the antimicrobial effect of 2% chlorhexidine, 2.5% sodium hypochlorite and mums containing 2% chlorhexidine. | 2012 | 23151688 |
| ph and antimicrobial activity of portland cement associated with different radiopacifying agents. | objective. the aim of this study was to evaluate the antimicrobial activity and ph changes induced by portland cement (pc) alone and in association with radiopacifiers. methods. the materials tested were pure pc, pc + bismuth oxide, pc + zirconium oxide, pc + calcium tungstate, and zinc oxide and eugenol cement (zoe). antimicrobial activity was evaluated by agar diffusion test using the following strains: micrococcus luteus, streptococcus mutans, enterococcus faecalis, pseudomonas aeruginosa, an ... | 2012 | 23119173 |
| is chlorhexidine an ideal vehicle for calcium hydroxide? a microbiologic review. | microorganisms play a major role in the initiation and perpetuation of pulpal and periapical disease. in order to predictably achieve a bacteria-free root canal system, it is necessary to use intracanal medicaments. calcium hydroxide [ca(oh)(2)] is the most common intracanal medicaments. it is effective against primary infections. however, its effectiveness against enterococcus (e.) faecalis and candida (c.) albicans is controversial. on the other hand, chlorhexidine (chx) is a potent agent agai ... | 2012 | 23056129 |
| the effect of heat-killed candida albicans and dentin powder on the antibacterial activity of chlorhexidine solution. | the purpose of this study was to compare the inhibitory effect of heat-killed candida albicans and dentin powder on the antibacterial activity of chlorhexidine (chx) against enterococcus faecalis and streptococcus sanguis. | 2012 | 23056121 |
| determination of the total phenolic and anthocyanin contents and antimicrobial activity of viburnum opulus fruit juice. | viburnum opulus is a plant with fruits that are rich in biologically active substances, making it valuable to the food and pharmaceutical industries. here, we present our study of the total phenolic and anthocyanin contents and antimicrobial activity in the fruit juice of six v. opulus l. accessions. the cultivar 'krasnaya grozd' was notable for its exceptionally large amount of total phenolics, 1168 mg/100 g, with anthocyanins comprising 3-5 % of the total phenolic content. the evaluation of th ... | 2012 | 22865031 |
| in vitro evaluation of the antimicrobial activity of a range of probiotics against pathogens: evidence for the effects of organic acids. | the aim of this study was to investigate the antimicrobial properties of fifteen selected strains belonging to the lactobacillus, bifidobacterium, lactococcus, streptococcus and bacillus genera against gram-positive and gram-negative pathogenic bacteria. in vitro antibacterial activity was initially investigated by an agar spot method. results from the agar spot test showed that most of the selected strains were able to produce active compounds on solid media with antagonistic properties against ... | 2012 | 22959627 |
| distribution of the vang-like gene cluster in clostridium difficile clinical isolates. | treatment of clostridium difficile infections generally requires cessation of their causative antibiotic and subsequent administration of metronidazole or vancomycin. intriguingly, the genome of c. difficile 630 contains a cryptic gene cluster homologous to the vang-type operon of enterococcus faecalis bm4518. we detected this cluster by pcr in 35 out of 41 clinical isolates, confirming its large prevalence in this species. the cluster was found to be located in a unique locus. comparison of thi ... | 2012 | 22409319 |
| activation of type iii interferon genes by pathogenic bacteria in infected epithelial cells and mouse placenta. | bacterial infections trigger the expression of type i and ii interferon genes but little is known about their effect on type iii interferon (ifn-λ) genes, whose products play important roles in epithelial innate immunity against viruses. here, we studied the expression of ifn-λ genes in cultured human epithelial cells infected with different pathogenic bacteria and in the mouse placenta infected with listeria monocytogenes. we first showed that in intestinal lovo cells, induction of ifn-λ genes ... | 2012 | 22720036 |
| candida albicans and bacterial microbiota interactions in the cecum during recolonization following broad-spectrum antibiotic therapy. | candida albicans is a normal member of the gastrointestinal (gi) tract microbiota of healthy humans, but during host immunosuppression or alterations in the bacterial microbiota, c. albicans can disseminate and cause life-threatening illness. the bacterial microbiome of the gi tract, including lactic acid bacteria (lab), plays a vital role in preventing fungal invasion. however, little is known about the role of c. albicans in shaping the bacterial microbiota during antibiotic recovery. we inves ... | 2012 | 22778094 |
| biochemical characterization of an anti-candida factor produced by enterococcus faecalis. | because candida albicans is resistant to several antifungal antibiotics, there is a need to identify other less toxic natural products, particularly antimicrobial proteins, peptides or bacteriocin like inhibitory substances. an attempt has been made to purify and characterise an anti-candida compound produced by enterococcus faecalis. | 2012 | 22759735 |
| inhibition of candida albicans yeast-hyphal transition and biofilm formation by solidago virgaurea water extracts. | xerostomia is a decrease of saliva secretion, which can unbalance the oral microflora, mainly to the benefit of candida albicans. the aim of the present study was to find a plant extract that could create an unfavourable environment for candida, and would, therefore, be appropriate for use in a dry-mouth daily-care mouthwash. water extract from the herbaceous plant solidago virgaurea (goldenrod) was selected due to its saponin content (plant detergents). saponin concentrations reached 0.7 and 0. ... | 2012 | 22422572 |
| assessment of the antimicrobial activity of acellular vascular grafts. | several studies have reported biological vascular grafts to be more resistant to microbial infection than synthetic counterparts in vivo. indeed, small intestinal submucosa (sis) materials have previously been reported to be antimicrobial. the aim of this study was to assess the antimicrobial activity and the ability to resist biofilm formation of a novel acellular vascular graft and compare it to commercially available alternatives using a range of organisms: mrsa, mssa, staphylococcus epidermi ... | 2012 | 22340962 |
| the effect of various concentrations of iodine potassium iodide on the antimicrobial properties of mineral trioxide aggregate--a pilot study. | mineral trioxide aggregate (mta) is a multi-purpose dental material with various uses in dentistry. iodine potassium iodide (iki) is the most commonly used iodine compound in endodontics. we aimed to assess the antimicrobial activity of tooth-colored proroot mta combined with iki. | 2012 | 22296180 |
| polymerase chain reaction of enterococcus faecalis and candida albicans in apical periodontitis from turkish patients. | the aim of this study was to determine the frequency of two important pathogenic microorganisms associated with endodontic infections, enterococcus faecalis and candida albicans, in root canal samples from patients with necrotic pulps or failed canal therapy by polymerase chain reaction method. | 2012 | 24558522 |
| chemical composition and biological activities of essential oil from hyptis crenata growing in the brazilian cerrado. | essential oils from species of the genus hyptis are well-known for their significant biological properties, including antimicrobial and acaricidal activities. the essential oil from the aerial parts of h. crenata was obtained by hydrodistillation; bomeol (17.8%), 1,8-cineol (15.6%) and p-cimene (7.9%) were characterized by gc-ms as its major constituents. the essential oil was evaluated in vitro for its antimicrobial activities against six fungal and five bacterial strains, by measuring the resp ... | 2012 | 23157018 |
| antimicrobial activity of some medicinal plants from the cerrado of the centralwestern region of brazil. | ethanol extracts from six selected species from the cerrado of the central-western region of brazil, which are used in traditional medicine for the treatment of infectious diseases and other medical conditions, namely erythroxylum suberosum st. hil. (erythroxylaceae), hyptis crenata pohl. ex benth. (lamiaceae), roupala brasiliensis klotz. (proteaceae), simarouba versicolor st. hil. (simaroubaceae), guazuma ulmifolia lam. (sterculiaceae) and protium heptaphyllum (aubl.) march. (burseraceae), as w ... | 2012 | 24031956 |
| a shift from colon- to ileum-predominant bacteria in ileal-pouch feces following total proctocolectomy. | we previously investigated fecal flora of the pouch after total proctocolectomy using terminal restriction fragment polymorphism analysis. although the results of the cluster analysis demonstrated clearly that bacterial populations, including an unidentified bacteria generating a 213-bp pcr fragment, moved toward a colon-like community in the pouch, it did not track changes in the individual species of fecal bacteria. | 2012 | 22539039 |
| screening for lactic acid bacteria capable of inhibiting campylobacter jejuni in in vitro simulations of the broiler chicken caecal environment. | thermotolerant campylobacter spp., specifically campylobacter jejuni and campylobacter coli, are the most common bacterial causes of human gastroenteritis in developed countries. consumption of improperly prepared poultry products and cross contamination are among the main causes of human campylobacteriosis. the aim of this study was to identify lactic acid bacterial (lab) strains capable of inhibiting c. jejuni growth in initial in vitro trials ('spot-on-lawn' method), as well as in batch ferme ... | 2012 | 23234730 |
| a microbiological profile of unexposed and exposed pulp space of primary endodontic infections by checkerboard dna-dna hybridization. | the aim of this study was to evaluate, by checkerboard dna-dna hybridization, the composition of the microbiota of primary endodontic infections in cases associated with exposed (n = 30) and unexposed (n = 30) pulp space. | 2012 | 22703649 |
| evaluation of impact of exposure of sudan azo dyes and their metabolites on human intestinal bacteria. | sudan azo dyes are banned for food usage in most countries, but they are illegally used to maintain or enhance the color of food products due to low cost, bright staining, and wide availability of the dyes. in this report, we examined the toxic effects of these azo dyes and their potential reduction metabolites on 11 prevalent human intestinal bacterial strains. among the tested bacteria, cell growth of 2, 3, 5, 5, and 1 strains was inhibited by sudan i, ii, iii, iv, and para red, respectively. ... | 2012 | 22634331 |
| [antimicrobial susceptibility of clinical isolates of aerobic gram-positive cocci and anaerobic bacteria in 2008]. | the activity of antibacterial agents against aerobic gram-positive cocci (25 genus or species, 1029 strains) and anaerobic bacteria (21 genus or species, 187 strains) isolated from clinical specimens in 2008 at 16 clinical facilities in japan were studied using either broth microdilution or agar dilution method. the ratio of methicillin-resistant strains among staphylococcus aureus and staphylococcus epidermidis was 59.6% and 81.2%, suggesting that resistant strains were isolated at high frequen ... | 2012 | 22808693 |
| ceftaroline fosamil in the treatment of community-acquired bacterial pneumonia and acute bacterial skin and skin structure infections. | ceftaroline fosamil is a cephalosporin antibacterial approved by the us food and drug administration (fda) for use in the treatment of acute bacterial skin and skin structure infections (absssi) and community-acquired bacterial pneumonia (cabp). after intravenous administration, ceftaroline fosamil is rapidly converted to its bioactive metabolite, ceftaroline. ceftaroline has broad-spectrum in vitro activity against gram-positive and gram-negative bacteria, including contemporary resistant gram- ... | 2012 | 22779432 |
| in vitro development and transfer of resistance to chlortetracycline in bacillus subtilis. | the present criteria and rules controlling the approval of the use of probiotics are limited to antibiotic resistance patterns and the presence of antibiotic resistance genes in bacteria. there is little information available in the literature regarding the risk of the usage of probiotics in the presence of antibiotic pressure. in this study we investigated the development and transfer of antibiotic resistance in bacillus subtilis selected in vitro by chlortetracycline in a stepwise manner. baci ... | 2012 | 23124749 |
| antibiotic producing microorganisms from river wiwi, lake bosomtwe and the gulf of guinea at doakor sea beach, ghana. | microorganisms have provided a wealth of metabolites with interesting activities such as antimicrobial, antiviral and anticancer. in this study, a total of 119 aquatic microbial isolates from 30 samples (taken from water bodies in ghana) were screened by the agar-well diffusion method for ability to produce antibacterial-metabolites. | 2012 | 23072432 |
| can artisanal "coalho" cheese from northeastern brazil be used as a functional food? | brazilian artisanal "coalho" cheeses from six northeast towns were investigated as a functional food based on their peptide profiles and antioxidant, zinc-binding and antimicrobial activities. the peptides (wsp) from "coalho" cheese showed high antioxidant activity, the best value of teac being 2223±10.10μm, which means 91.1±0.43% oxidative inhibition and peptide concentration for ic(50) of 7mg/ml (21μg of peptides) for sample from the town of correntes. the smallest teac value (1896±17μm), whic ... | 2012 | 22953890 |
| synthesis and antimicrobial activities of oximes derived from o-benzylhydroxylamine as fabh inhibitors. | forty-three oxime derivatives were synthesized by allowing o-benzylhydroxylamines to react with primary benzaldehydes or salicylaldehydes; these products were gauged as potential inhibitors of β-ketoacyl-(acyl-carrier-protein) synthase iii (fabh). among the 43 compounds, 38 are reported herein for the first time. these compounds were assayed for antimicrobial activities against escherichia coli, pseudomonas aeruginosa, pseudomonas fluorescens, bacillus subtilis, staphylococcus aureus, and entero ... | 2012 | 22811397 |
| two streptothricins with a cis-streptolidine lactam moiety from streptomyces sp. i08a 1776. | two unique cis-fused streptothricins (1 and 2) were isolated from the culture broth of streptomyces sp. i08a 1776. their structures were determined by ms, cd, and 1d and 2d nmr spectroscopic data analysis. compound 2 showed weak antibacterial activities against bacillus subtilis and enterococcus faecalis with mic values of 32 and 64 μg ml(-1), respectively. | 2012 | 22781281 |
| caenorhabditis elegans immune conditioning with the probiotic bacterium lactobacillus acidophilus strain ncfm enhances gram-positive immune responses. | although the immune response of caenorhabditis elegans to microbial infections is well established, very little is known about the effects of health-promoting probiotic bacteria on evolutionarily conserved c. elegans host responses. we found that the probiotic gram-positive bacterium lactobacillus acidophilus ncfm is not harmful to c. elegans and that l. acidophilus ncfm is unable to colonize the c. elegans intestine. conditioning with l. acidophilus ncfm significantly decreased the burden of a ... | 2012 | 22585961 |
| syntheses and biological studies of novel spiropiperazinyl oxazolidinone antibacterial agents using a spirocyclic diene derived acylnitroso diels-alder reaction. | several novel oxazolidinone antibiotics with a spiropiperazinyl substituent at the 4'-position of the phenyl ring were synthesized through nitroso diels-alder chemistry and the in vitro antibacterial activities were evaluated against various gram-positive bacteria (bacillus subtilis, staphylococcus aureus, enterococcus faecalis), gram-negative bacteria (escherichia coli, pseudomonas aeruginosa) and mycobacteria (mycobacterium vaccae, mycobacterium tuberculosis). analogs (8a and 12) were active a ... | 2012 | 22560837 |
| isolation and characterization of signermycin b, an antibiotic that targets the dimerization domain of histidine kinase walk. | the walk (histidine kinase)/walr (response regulator) two-component signal transduction system is a master regulatory system for cell wall metabolism and growth. this system is conserved in low g+c gram-positive bacteria, including bacillus subtilis, staphylococcus aureus, enterococcus faecalis, and streptococcus mutans. in this study, we found the first antibiotic that functions as a walk inhibitor (signermycin b) by screening 10,000 streptomyces extracts. the chemical structure (c(23)h(35)no(4 ... | 2012 | 22526318 |
| indirect rapid prototyping of antibacterial acid anhydride copolymer microneedles. | microneedles are needle-like projections with microscale features that may be used for transdermal delivery of a variety of pharmacologic agents, including antibacterial agents. in the study described in this paper, an indirect rapid prototyping approach involving a combination of visible light dynamic mask micro-stereolithography and micromolding was used to prepare microneedle arrays out of a biodegradable acid anhydride copolymer, gantrez(®) an 169 bf. fourier transform infrared spectroscopy, ... | 2012 | 22287512 |
| antimicrobial activity of carpolobia lutea extracts and fractions. | carpolobia lutea (g. don) (polygalaceae) is a tropical medicinal plant putative in traditional medicines against gonorrhea, gingivitis, infertility, antiulcer and malaria. the present study evaluated the antimicrobial, antifungal and antihelicobacter effects of extracts c. lutea leaf, stem and root. the extracts were examined using the disc-diffusion and microplates of 96 wells containing muller-hinton methods against some bacterial strains: eschericia coli (atcc 25922), e. coli (atcc10418), pse ... | 2012 | 23983362 |
| nationwide surveillance of antimicrobial susceptibility patterns of pathogens isolated from surgical site infections (ssi) in japan. | to investigate the trends of antimicrobial resistance in pathogens isolated from surgical site infections (ssi), a japanese surveillance committee conducted the first nationwide survey. seven main organisms were collected from ssi at 27 medical centers in 2010 and were shipped to a central laboratory for antimicrobial susceptibility testing. a total of 702 isolates from 586 patients with ssi were included. staphylococcus aureus (20.4 %) and enterococcus faecalis (19.5 %) were the most common iso ... | 2012 | 23143280 |
| the constituents of essential oil: antimicrobial and antioxidant activity of micromeria congesta boiss. & hausskn. ex boiss. from east anatolia. | the chemical composition, antimicrobial activity, total phenol content, total antioxidant activity, and total oxidant status of the essential oil from micromeria congesta boiss. & hausskn. ex boiss. were investigated. steam distillation was used to obtain the essential oil, and the chemical analyses were performed by gas chromatography-mass spectrometry. the antimicrobial activity was tested by an agar disc diffusion method against the tested microorganisms: bacillus subtilis nrrl b-744, bacillu ... | 2012 | 22871061 |
| composition and antimicrobial activity of fatty acids detected in the hygroscopic secretion collected from the secretory setae of larvae of the biting midge forcipomyia nigra (diptera: ceratopogonidae). | the hygroscopic secretion produced by the secretory setae of terrestrial larvae of the biting midge forcipomyia nigra (winnertz) was analysed using gas chromatography coupled with mass spectrometry (gc-ms). the viscous secretion is stored at the top of each seta and absorbs water from moist air. gc-ms analyses (four independent tests) showed that the secretion contained 12 free fatty acids, the most abundant of which were oleic (18:1), palmitic (16:0), palmitoleic (16:1) and linoleic (18:2). oth ... | 2012 | 22781366 |
| fluorescence-based comparative evaluation of bactericidal potency and food application potential of anti-listerial bacteriocin produced by lactic acid bacteria isolated from indigenous samples. | the aim of the present study was to ascertain the potency of anti-listerial bacteriocin produced by lactic acid bacteria (lab) isolated from indigenous samples of dahi, dried fish, and salt-fermented cucumber. a total of 231 lab isolates were obtained from the samples, of which 51 isolates displayed anti-listerial activity. the anti-listerial lab were identified by pcr as lactobacillus sp., pediococcus sp., enterococcus sp., and lactococcus sp. pcr also enabled the detection of class iia bacteri ... | 2012 | 26781854 |
| [antibacterial activity of rare streptomyces species against clinical resistant bacteria]. | in the search for new antibiotics from steptomyces, investigating extremes habitats enhances the probability of isolating novel producers. in this context, the antibacterial activity of four streptomyces strains isolated from ezzmoul saltpans was studied. two of them showed antibacterial activity against antibiotic's resistant bacteria (bacillus cereus: β-lactamines and sulfamides resistant, streptococcus faecalis: penicillin, tetracycline and cotrimoxazole resistant, and staphylococcus aureus m ... | 2012 | 22484527 |
| novel bacterial lipoprotein structures conserved in low-gc content gram-positive bacteria are recognized by toll-like receptor 2. | bacterial lipoproteins/lipopeptides inducing host innate immune responses are sensed by mammalian toll-like receptor 2 (tlr2). these bacterial lipoproteins are structurally divided into two groups, diacylated or triacylated lipoproteins, by the absence or presence of an amide-linked fatty acid. the presence of diacylated lipoproteins has been predicted in low-gc content gram-positive bacteria and mycoplasmas based on the absence of one modification enzyme in their genomes; however, we recently d ... | 2012 | 22303020 |
| synthesis and biological evaluation of some novel 3,5-disubstituted-1,2,4-triazole incorporated 2-mercaptobenzothiazoles. | several 2-mercaptobenzothiazole derivatives 5a-i containing 1,2,4-triazole moiety incorporating two additional substituents were synthesized. all the newly synthesized compounds were tested for in vitro activity against certain strains of bacteria such as enterococcus faecalis, bacillus coagulans, pseudomonas aeruginosa, escherichia coli and candida albicans. compound 5a showed significant activity against the gram-negative bacteria escherichia coli. compounds 5a-i were also screened for their a ... | 2012 | 24061354 |
| addition of probiotics to norfloxacin does not improve efficacy in the prevention of spontaneous bacterial peritonitis: a double-blind placebo-controlled randomized-controlled trial. | spontaneous bacterial peritonitis (sbp) may occur despite antibiotic prophylaxis. we investigated whether the addition of probiotics to norfloxacin enhances its efficacy in the prevention of sbp. | 2012 | 22522141 |
| phytochemical profiles of volatile constituents from centaurea ragusina leaves and flowers and their antimicrobial effects. | the volatile oils obtained from the leaves and flowers of the croatian endemic plant centaurea ragusina l. were evaluated for antimicrobial activity. the chemical compositions, determined by gc and gc-ms, were complex, with sesquiterpenes the most abundant class of compound. nonterpene hydrocarbons were also identified in high quantities, while acids and ketones were found in high quantities only in the leaf volatile oil. aldehydes, alcohols, esters and other compounds were identified in lower q ... | 2012 | 22978236 |
| antibacterial effect of phosphates and polyphosphates with different chain length. | the aim of this study was to monitor the antibacterial effect of seven phosphate salts on selected strains of gram-negative and gram-positive bacteria, which could be considered responsible for food-borne diseases (bacillus cereus, bacillus subtilis, enterococcus faecalis, micrococcus luteus, staphylococcus aureus, citrobacter freundii, escherichia coli, proteus mirabilis, salmonella enterica ser. enteritidis and pseudomonas aeruginosa). for these purposes, phosphates differing in chain length w ... | 2012 | 22934995 |
| comparative analysis of classical and molecular microbiology methods for the detection of escherichia coli and enterococcus spp. in well water. | the microbiological quality of 165 1 litre well water samples collected in the québec city region was assessed by culture-based methods (mfc agar, chromocult coliform agar, colilert(®), mi agar, chromocult enterococci, enterolert™, and mei agar) and by a molecular microbiology strategy, dubbed crename-rtpcr, developed for the detection of escherichia coli, enterococcus spp., enterococcus faecalis/faecium, and bacillus atrophaeus subsp. globigii. in these drinking water samples, approved culture- ... | 2012 | 23014932 |
| antimicrobial and synergistic effects of silver nanoparticles synthesized using soil fungi of high altitudes of eastern himalaya. | fifty three fungi isolated from soils of different microhabitats of eastern himalayan range (3,400~3,600 msl) were screened for mycosynthesis of silver nanaoparticles (agnps) and their efficacy as antimicrobials were assessed in combination with commonly used antibiotics. three isolates aspergillus terreus sp5, paecilomyces lilacinus sf1 and fusarium sp. mp5 identified based on morphological and 18s rrna gene sequences were found to synthesize agnps. these nanoparticles were characterized by vis ... | 2012 | 22783131 |
| the antimicrobial, antioxidative, anti-inflammatory activity and cytotoxicity of different fractions of four south african bauhinia species used traditionally to treat diarrhoea. | many bauhinia species, including those indigenous to south africa, are used in traditional medicine across the world for treating ailments such as gastrointestinal tract (git) disorders, diabetes, infectious diseases and inflammation. | 2012 | 22917809 |
| cytotoxicity and antimicrobial activity of the methanol extract and compounds from polygonum limbatum. | the present study was designed to investigate the antimicrobial activity and the cytotoxicity of the methanol extract (pla) as well as fractions (pla1-4) and compounds [cardamomin (1), (±)-polygohomoisoflavanone (2), (s)-(-)-pinostrobin (3), 2',4'-dihydroxy-3',6'-dimethoxychalcone (4), (2s)-(-)-5-hydroxy-6,7-dimethoxyflavanone (5), and (2s)-(-)-5,7-dimethoxyflavanone (6)] obtained from leaves of polygonum limbatum. the microbroth dilution was used to determine the minimal inhibitory concentratio ... | 2012 | 22495442 |
| phylogenetic analysis of antimicrobial lactic acid bacteria from farmed seabass dicentrarchus labrax. | the use of lactic acid bacteria (lab) in the prevention or reduction of fish diseases is receiving increasing attention. in the present study, 47 lab strains were isolated from farmed seabass ( dicentrarchus labrax ) and were phenotypically and phylogenetically analysed by 16s rdna and randomly amplified polymorphic dna - polymerase chain reaction (rapd-pcr). their antimicrobial effect was tested in vitro against a wide variety of pathogenic and spoilage bacteria. most of the strains isolated we ... | 2012 | 22439634 |
| bloodstream infections in immunocompromised hosts. | immunocompromised hosts are at high risk for bloodstream infections (bsis); the outcome is unpredictable (even with adequate therapy), frequently severe (mortality 27%). a 26 months survey in a haematology/oncology hospital was set-up; it comprised 158 patients and detected 171 positive blood cultures. the origin of the infections was primitive in 27.22% and secondary in 72.78% of the cases; the most frequent cause was the presence of a vascular catheter (23.41%). the second most important cause ... | 2012 | 23210322 |
| human serum albumin stabilized gold nanoclusters as selective luminescent probes for staphylococcus aureus and methicillin-resistant staphylococcus aureus. | in this work, human serum albumin (hsa) stabilized gold nanoclusters (hsa-auncs) with reddish photoluminescence were used as sensing probes for pathogenic bacteria including enterobacter cloacae, escherichia coli j96, pseudomonas aeruginosa, pandrug-resistant acinetobacter baumannii (pdrab), staphylococcus aureus, methicillin-resistant s. aureus (mrsa), streptococcus pyogenes, and vancomycin-resistant enterococcus faecalis (vre). we discovered that hsa-auncs have unique affinity with s. aureus a ... | 2012 | 23088348 |
| the effect of a wound care solution containing polyhexanide and betaine on bacterial counts: results of an in vitro study . | polyhexanide and betaine topical solution is used in the management of infected wounds as a cleaning agent. an in vitro study was conducted to examine the antimicrobial effects of a solution containing 0.1% of the antimicrobial agent polyhexanide and 0.1% of the surfactant betaine. three batches of each product were tested, and culture results of 13 microorganisms were evaluated after 7, 14, and 28 days using usp <51> methodology. growth reduction was identical at each day following exposure to ... | 2012 | 23037330 |
| [analysis of distribution characteristics and drug resistance of 2748 strains of pathogens isolated from burn patients]. | to provide epidemiological data of the distribution characteristics and drug resistance of the pathogens isolated from burn patients in recent years for guiding rational use of antibiotics in clinic. | 2012 | 22781318 |
| reproducible discrimination between gram-positive and gram-negative bacteria using surface enhanced raman spectroscopy with infrared excitation. | the on time diagnostics of bacterial diseases is one of the essential steps in the foregoing treatment of such pathogens. here we sought to present an easy to use and robust method for the discrimination between gram-positive (enterococcus faecalis and streptococcus pyogenes) and gram-negative (acinetobacter baumannii and klebsiella pneumoniae) bacterial genera based on surface enhanced raman scattering (sers) spectroscopy. the robustness of our approach lies in the novel method for the producti ... | 2012 | 22577658 |
| garlic ointment inhibits biofilm formation by bacterial pathogens from burn wounds. | when thermal injury damages the skin, the physical barrier protecting underlying tissues from invading micro-organisms is compromised and the host's immune system becomes supressed, facilitating colonization and infection of burn wounds with micro-organisms. within the wound, bacteria often develop biofilms, which protect the bacteria from the immune response and enhance their resistance to antibiotics. as the prophylactic use of conventional antibiotics drives selection of drug-resistant strain ... | 2012 | 22301617 |
| in vitro evaluation of mtad and nisin in combination against common pathogens associated with root canal infection. | many pathogenic microorganisms were found in an infected root canal. the object of this study was to evaluate the effect of mtad in combination with nisin on the pathogens associated with root canal infection. | 2012 | 22414835 |
| development of a multispecies biofilm community by four root canal bacteria. | the development of multispecies biofilm models are needed to explain the interactions that take place in root canal biofilms during apical periodontitis. the aim of this study was to investigate the ability of 4 root canal bacteria to establish a multispecies biofilm community and to characterize the main structural, compositional, and physiological features of this community. | 2012 | 22341068 |
| daptomycin resistance in enterococci is associated with distinct alterations of cell membrane phospholipid content. | the lipopeptide antibiotic, daptomycin (dap) interacts with the bacterial cell membrane (cm). development of dap resistance during therapy in a clinical strain of enterococcus faecalis was associated with mutations in genes encoding enzymes involved in cell envelope homeostasis and phospholipid metabolism. here we characterized changes in cm phospholipid profiles associated with development of dap resistance in clinical enterococcal strains. | 2012 | 22952824 |
| whole-genome analysis of a daptomycin-susceptible enterococcus faecium strain and its daptomycin-resistant variant arising during therapy. | development of daptomycin (dap) resistance in enterococcus faecalis has recently been associated with mutations in genes encoding proteins with two main functions: (i) control of the cell envelope stress response to antibiotics and antimicrobial peptides (liafsr system) and (ii) cell membrane phospholipid metabolism (glycerophosphoryl diester phosphodiesterase and cardiolipin synthase [cls]). however, the genetic bases for dap resistance in enterococcus faecium are unclear. we performed whole-ge ... | 2012 | 23114757 |
| activity of moxifloxacin, imipenem, and ertapenem against escherichia coli, enterobacter cloacae, enterococcus faecalis, and bacteroides fragilis in monocultures and mixed cultures in an in vitro pharmacokinetic/pharmacodynamic model simulating concentrations in the human pancreas. | the activities of moxifloxacin, imipenem, and ertapenem against pathogens causing severe necrotizing pancreatitis were studied in an in vitro pharmacokinetics/pharmacodynamics (pk/pd) model. escherichia coli, enterobacter cloacae, enterococcus faecalis, and bacteroides fragilis were exposed in monocultures and mixed cultures to concentrations of the three agents comparable to those in the human pancreas. moxifloxacin was more active than the two carbapenems in monocultures and mixed cultures, re ... | 2012 | 23070164 |
| acute gastrointestinal dilation in laboratory rhesus monkeys in the korea national primate research center. | acute gastrointestinal dilation is a medical condition in which the stomach and intestine become overstretched by excessive gas content. in laboratory monkeys, cases of bloating involving gastrointestinal dilation are rarely seen, and the cause thereof is not clearly defined. two rhesus monkeys in the korea national primate research center were found to suffer from acute gastrointestinal dilation. one of the monkeys showed severe gastric bloating after recovering from general anesthesia with iso ... | 2012 | 23091523 |
| muraa is required for intrinsic cephalosporin resistance of enterococcus faecalis. | enterococcus faecalis is a low-gc gram-positive bacterium that is intrinsically resistant to cephalosporins, antibiotics that target cell wall biosynthesis. to probe the mechanistic basis for intrinsic resistance, a library of transposon mutants was screened to identify e. faecalis strains that are highly susceptible to ceftriaxone, revealing a transposon mutant with a disruption in muraa. muraa is predicted to encode a udp-n-acetylglucosamine 1-carboxyvinyl transferase that catalyzes the first ... | 2012 | 22290954 |
| endocarditis in a large district general hospital: a study of the microbiological spectrum between 2000 and 2011. | infective endocarditis is one of three common cardiac infections in the united kingdom, in addition to myocarditis and pericarditis, with a reported incidence of 1.7 to 6.2 cases per 100,000 patient years. infective endocarditis can often have serious consequences and a wide variety of organisms may be the causative pathogen. there are little published data regarding the exact spectrum of organisms that cause endocarditis in the united kingdom and whether organisms such as streptococci still dom ... | 2012 | 23592930 |
| evaluation of a therapeutic vaccine for the prevention of recurrent urinary tract infections versus prophylactic treatment with antibiotics. | urinary tract infections (utis) are considered the most common bacterial infections, especially in women. the objective of this study was to evaluate the use of the sublingual bacterial vaccine uromune® in order to prevent recurrent utis (rutis). | 2012 | 22806485 |
| clinical identification of bacteria in human chronic wound infections: culturing vs. 16s ribosomal dna sequencing. | chronic wounds affect millions of people and cost billions of dollars in the united states each year. these wounds harbor polymicrobial biofilm communities, which can be difficult to elucidate using culturing methods. clinical molecular microbiological methods are increasingly being employed to investigate the microbiota of chronic infections, including wounds, as part of standard patient care. however, molecular testing is more sensitive than culturing, which results in markedly different resul ... | 2012 | 23176603 |
| structural insight into the clostridium difficile ethanolamine utilisation microcompartment. | bacterial microcompartments form a protective proteinaceous barrier around metabolic enzymes that process unstable or toxic chemical intermediates. the genome of the virulent, multidrug-resistant clostridium difficile 630 strain contains an operon, eut, encoding a bacterial microcompartment with genes for the breakdown of ethanolamine and its utilisation as a source of reduced nitrogen and carbon. the c. difficile eut operon displays regulatory genetic elements and protein encoding regions in co ... | 2012 | 23144756 |
| bactericidal efficacy of elevated ph on fish pathogenic and environmental bacteria. | ship ballast water is a recognized medium for transfer and introductions of nonindigenous species. there is a need for new ballast water treatment methods that effectively and safely eliminate or greatly minimize movements of these species. the present study employed laboratory methods to evaluate the bactericidal efficacy of increased ph (ph 10.0-12.0) for exposure durations of up to 72 h to kill a variety of gram-negative and gram-positive bacteria including fish pathogens (aeromonas spp., yer ... | 2012 | 25685439 |
| antiadhesive activity of the biosurfactant pseudofactin ii secreted by the arctic bacterium pseudomonas fluorescens bd5. | pseudofactin ii is a recently identified biosurfactant secreted by pseudomonas fluorescens bd5, the strain obtained from freshwater from the arctic archipelago of svalbard. pseudofactin ii is a novel compound identified as cyclic lipopeptide with a palmitic acid connected to the terminal amino group of eighth amino acid in peptide moiety. the c-terminal carboxylic group of the last amino acid forms a lactone with the hydroxyl of thr3. adhesion is the first stage of biofilm formation and the best ... | 2012 | 22360895 |
| enterococcus faecalis prgj, a virb4-like atpase, mediates pcf10 conjugative transfer through substrate binding. | the enterococcus faecalis prg and pcf genes of plasmid pcf10 encode a type iv secretion system (t4ss) required for conjugative transfer. prgj is a member of the virb4 family of atpases that are universally associated with t4sss. here, we report that purified prgj dimers displayed atp binding and hydrolysis activities. a prgj nucleoside triphosphate (ntp) binding site mutation (k471e) slightly diminished atp binding but abolished atp hydrolysis in vitro and blocked pcf10 transfer in vivo. as show ... | 2012 | 22636769 |
| involvement of peptidylprolyl cis/trans isomerases in enterococcus faecalis virulence. | peptidylprolyl cis/trans isomerases (ppiases) are enzymes involved in protein folding. analysis of the genome sequence of enterococcus faecalis v583 allowed for identification of 3 ppiases carrying genes. ef2898 encodes an intracellular ppiase which was not shown to be important for the e. faecalis stress response or virulence. the other two ppiases, the parvulin family rotamase ef0685 and the cyclophilin family member ef1534, are expected to be surface-exposed proteins. they were shown to be im ... | 2012 | 22331431 |
| investigation of antimicrobial activity and statistical optimization of bacillus subtilis spb1 biosurfactant production in solid-state fermentation. | during the last years, several applications of biosurfactants with medical purposes have been reported. biosurfactants are considered relevant molecules for applications in combating many diseases. however, their use is currently extremely limited due to their high cost in relation to that of chemical surfactants. use of inexpensive substrates can drastically decrease its production cost. here, twelve solid substrates were screened for the production of bacillus subtilis spb1 biosurfactant and t ... | 2012 | 22536017 |
| exploring optimization parameters to increase ssdna recombineering in lactococcus lactis and lactobacillus reuteri. | single-stranded dna (ssdna) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. cells which express a single-stranded dna binding protein (rect or bet) are transformed with an oligonucleotide which is incorporated via an annealing and replication-dependent mechanism. by in silico analysis we identified ssdna binding protein homologs in the genus lactobacillus and lactococcus lactis. to assess whether we could further improve the reco ... | 2012 | 22750793 |
| development of 4h-pyridopyrimidines: a class of selective bacterial protein synthesis inhibitors. | we have identified a series of compounds that inhibit protein synthesis in bacteria. initial ic50's in aminoacylation/translation (a/t) assays ranged from 3 to14 μm. this series of compounds are variations on a 5,6,7,8-tetrahydropyrido[4,3-d]pyrimidin-4-ol scaffold (e.g., 4h-pyridopyrimidine). | 2012 | 22373064 |