Publications

cell proliferation kinetics of epidermis, hair follicles, and sebaceous glands of cocker spaniels with idiopathic seborrhea.	cell proliferation kinetic values were established for the epidermis, hair follicle epithelium, and sebaceous glands of 8 cocker spaniels with primary idiopathic seborrhea. values were established by intradermal pulse labeling injections of tritiated thymidine followed by cutaneous biopsy and autoradiography. the epidermal basal cell-labeling index was 4.96 +/- 0.97%, and the epidermal nucleated cell-labeling index was 3.33 +/- 0.71%. calculated epidermal cell renewal time for the viable layers ...	1989	2533468
bile acid profile in a dog with cholangiocarcinoma.		1989	2536512
a simple colorimetric assay method for pyrophosphate in the presence of a 1000-fold excess of orthophosphate: application of the method to the study of pyrophosphate metabolism in mitochondria.	a sensitive colorimetric method for the assay of inorganic pyrophosphate with excess of orthophosphate is described. the principle of this method lies in the formation of phosphomolybdate and ppi-molybdate complexes with subsequent extraction of the phosphomolybdate complex by organic solvents and reduction of the ppi-molybdate complex by dithiothreitol and eikonogen. the sensitivity of the method was from 5 to 120 nmol of ppi in a 2.0-ml sample.	1989	2545112
sodium and potassium currents recorded during an action potential.	a simple method was used to measure directly sodium and potassium currents underlying the action potential in single nerve fibres of xenopus laevis. a short rectangular stimulus under current-clamp conditions elicited an action potential which was digitally stored and later used as command when voltage-clamping the same fibre. the currents thus obtained nearly reproduced the original rectangular stimulus. adding first 100 nm ttx and subsequently 100 nm ttx plus 10 mm tea to the extracellular rin ...	1989	2546753
hypoxic changes in hippocampal neurons.	1. reversible effects of brief periods of anoxia (replacing 95% o2-5% co2 with 95% n2-5% co2 for 2-4 min) were studied in ca1 neurons in hippocampal slices (from sprague-dawley rats), kept in an interface-type chamber at 33.5 degree. 2. the predominant voltage change during anoxia (n2) was a hyperpolarization, accompanied by a marked fall in resistance and excitability; synaptic potentials were also depressed, especially inhibitory postsynaptic potentials (ipsps). 3. in voltage-current (v-i) plo ...	1989	2547034
enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus.	we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ...	1989	2556341
[acute maternal anterior poliomyelitis in a non-endemic zone].	the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus.	1989	2561040
comments on radiation-induced g2 arrest in mouse zygotes.		1989	2564879
in-vivo effects of (e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine (mdl 72145) on amine oxidase activities in the rat. selective inhibition of semicarbazide-sensitive amine oxidase in vascular and brown adipose tissues.	one hour after mdl 72145 ((e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine) (2.5 mg kg-1) was given by intraperitoneal injection, the semicarbazide-sensitive amine oxidase (ssao) activity of rat aorta and brown adipose tissue measured in-vitro was reduced by more than 95% of its control value, whereas the monoamine oxidase (mao-a) activity remained virtually unaffected. the action of this drug on amine oxidases in the liver at this dose was less selective. the in-vitro effect of mdl 72145 on the ...	1989	2565962
effects of toxin isolated from the sea anemone bolocera tuediae on electrical properties of isolated rat skeletal muscle and cultured myotubes.	electrophysiological effects of a polypeptide toxin isolated from the sea anemone bolocera tuediae (bttx ii) on isolated fast and slow rat skeletal muscle and on cultured rat myotubes are described in this paper. nanomolar concentrations of bttx ii cause a concentration-dependent depolarization. this effect is prevented by the presence of tetrodotoxin. action potential duration is prolonged in all preparations investigated, with the fast muscle being the least sensitive. the positive overshoot a ...	1989	2566207
detection of piluslike structures on clinical and environmental isolates of vibrio vulnificus.	twenty clinical isolates of vibrio vulnificus were compared with 10 environmental strains by using electron microscopy and agglutination assays with human erythrocytes, guinea pig erythrocytes, and saccharomyces cerevisiae. in addition, the isolates were tested for ability to adhere to the human epithelial cell lines hep-2 and a549. when examined by electron microscopy, 16 (80%) of the 20 clinical isolates demonstrated the presence of piluslike structures; the composition of the bacterial popula ...	1989	2568368
recombinant human erythropoietin treatment in patients on maintenance home haemodialysis.		1989	2570273
hydrogen peroxide mutagenicity towards salmonella typhimurium.	in bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ...	1989	2572065
characterization of rat cerebral cortical beta adrenoceptor subtypes using (-)-[125i]-iodocyanopindolol.	(-)-[125i]-iodocyanopindolol [-)icyp), used to characterize beta adrenoceptors on membrane preparations from rat cerebral cortex, was shown to have affinity for both beta adrenoceptors and serotonin receptors. therefore, 10 microm serotonin was added to the assays to prevent (-)icyp binding to serotonin receptors. under these conditions, (-)icyp binding to the cortical membrane preparation was reversible and saturable, and the association reaction was very slow. the dissociation reaction was als ...	1989	2577008
[serologic studies of listeriosis antibodies in dogs in greece].	in an epidemiological study of dogs carried out in greece, antibodies against listeriosis were demonstrated in the slow-agglutination and the complement-fixation-reaction. depending on the dogs' place of origin cfr titers were found in 6.1% to 51.1%. cfr-titers could be confirmed in a comparative investigation with the slow-agglutination-test.	1989	2583499
ultrastructure of the tail of echinostoma revolutum cercaria.	the ultrastructure of the tail fins and muscles of echinostoma revolutum cercaria is described. the longitudinal muscles consist of a primitive type of transversely striated muscle fibres with a well-developed sarcoplasmic reticulum in the z-band. the fins are formed by a longitudinal tegument fold containing the same components as the remaining part of the tail tegument. the difference between the body and tail tegument is discussed.	1989	2583613
pathological analyses on the compressed cord by the experimental spinal metastasis.	the purpose of the present experimental spinal metastasis developed in rats by inoculation of walker's cancer cells through the spinous process is to find the factor which causes the initial damage of the cord in this disorder. in the early stage of paralysis, microangiogram exhibited that the degenerated posterior funiculus originated from the small hemorrhagic area in the posterior column of the involved cord. scanning electron microscopic findings showed that the hemorrhage was of the intrins ...	1989	2586714
susceptibility of erythrocytes from several animal species to vibrio vulnificus hemolysin.	the hemolytic activity of vibrio vulnificus hemolysin (vvh) against erythrocytes from several animal species (sheep, horse, cow, rabbit, chicken) was investigated. vvh was active against erythrocytes from all species, but the amount of vvh causing 50% hemolysis under identical conditions (hemolytic susceptibility to vvh) differed. the degree of 125i-labeled vvh (125i-vvh) binding to each erythrocyte species correlated with the susceptibility of the cells to hemolysis. however, marked differences ...	1989	2612874
septicaemia due to vibrio vulnificus.		1989	2617638
survival of vibrio vulnificus in shellstock and shucked oysters (crassostrea gigas and crassostrea virginica) and effects of isolation medium on recovery.	when two species of shellstock oysters were artificially contaminated with vibrio vulnificus, the bacterium survived when the oysters were stored at 10 degrees c and below. large numbers of endogenous v. vulnificus cells were found after 7 days at both 0.5 and 10 degrees c in uninoculated control oysters (crassostrea virginica). oysters allowed to take up v. vulnificus from seawater retained the bacterium for 14 days at 2 degrees c. the presence of v. vulnificus in the drip exuded from the shell ...	1989	2619304
[rational treatment of necrotizing external otitis].	advances in the diagnosis and treatment of necrotizing external otitis (neo) during the past 20 years improved substantially the prognosis of this disease. the use of hyperbaric oxygen therapy greatly potentiates the effect of newly developed highly effective antibiotics. radioisotope examination of the petrous bones facilitates the diagnosis and indicates the time to terminate treatment. medicamentous treatment is preferable to surgical treatment, which is reserved for exceptional very advanced ...	1989	2631997
[rational treatment of necrotizing external otitis].	advances in the diagnosis and treatment of necrotizing external otitis (neo) during the past 20 years improved substantially the prognosis of this disease. the use of hyperbaric oxygen therapy greatly potentiates the effect of newly developed highly effective antibiotics. radioisotope examination of the petrous bones facilitates the diagnosis and indicates the time to terminate treatment. medicamentous treatment is preferable to surgical treatment, which is reserved for exceptional very advanced ...	1989	2631997
studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1).	anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ...	1989	2632089
studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1).	anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ...	1989	2632089
myofibroblasts in cotton-induced granulation tissue and the bovine adrenal capsule: morphological aspects.	ultrastructural studies of one week old cotton-induced granulation tissue revealed the presence of polymorphonuclear neutrophils (pmn), macrophages and mast cells, whilst the granulation tissue from two week old cotton implants contained fibroblasts, myofibroblasts and abundant collagen fibres. similarly, the bovine adrenal capsule contained both fibroblasts and myofibroblasts. it is proposed that the bovine adrenal capsule and two week cotton-induced granulation tissue could be utilized as in-v ...	1989	2634624
short chain fatty acid-induced colitis in mice.	short-chain fatty acids (e.g. acetic, propionic and butyric acid) are metabolic products produced by colonic bacteria. since intra-rectal instillation of dilute acetic acid to rats and cats has been reported to produce a diffuse colitis similar to the human disease, in our study we have investigated the effect of intra-rectal butyric acid in mice. male bka mice (n = 6) received 01 ml of dilute butyric acid by intra-rectal instillation which was flushed out with saline after 10 sec. control anima ...	1989	2634625
redirection of prostaglandin endoperoxide metabolism at the platelet-vascular interface in man.	prostacyclin (pgi2) is an inhibitor of platelet function in vitro. we tested the hypothesis that pgi2 is formed in biologically active concentrations at the platelet-vascular interface in man and can be pharmacologically modulated to enhance its inhibitory properties. this became feasible when we developed a microquantitative technique that permits the measurement of eicosanoids in successive 40-microliters aliquots of whole blood emerging from a bleeding time wound. in 13 healthy volunteers the ...	1989	2643628
internalization and degradation of insulin by a human insulin receptor-v-ros hybrid in chinese hamster ovary cells.	chinese hamster ovary cell lines expressing either the wild-type human insulin receptor or a hybrid molecule in which the tyrosine kinase domain of the insulin receptor is replaced with that of the oncogene, v-ros were examined for their ability to internalize and degrade insulin. cells expressing the hybrid receptor were found to internalize and degrade insulin at approximately half the rate of cells expressing the native insulin receptor. moreover, insulin was incapable of inducing the interna ...	1989	2645866
therapeutic implications of tumor heterogeneity.	at the outset of this review, we stated that we wished to raise some questions that challenge the commonly held view that tumor heterogeneity is of major significance to treatment failure. the nature of this challenge is the following: although tumor heterogeneity in sensitivity to therapeutic agents has been demonstrated repeatedly, using isolated subpopulations of cells, primarily in cell culture systems, there is very little work that has been directed toward asking the tough questions about ...	1989	2652316
detection of vibrio vulnificus cytolysin in v. vulnificus-infected mice.	enzyme-linked immunosorbent assays using polyclonal and monoclonal antibodies specific for v. vulnificus cytolysin detected the toxin in an extract of skin lesions and in serum from mice showing local and systemic v. vulnificus disease after subcutaneous injection of the bacterium. the cytolysin also was detected in skin lesions by an indirect immunofluorescence procedure using polyclonal and monoclonal antibodies. our findings provide direct evidence that the cytolysin is produced in vivo durin ...	1989	2658204
pulsed-field gel electrophoresis of circular dna.	mobility of supercoiled (form i) and nicked circular (form ii) plasmid dnas was determined on two major forms of pulsed-field electrophoresis, chef and ofage. plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with saccharomyces cerevisiae chromosomes as standards. agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels resolving forms i and ii of smaller plasmids. the pulsing range of 3.7 to 240 seconds resulted in quite v ...	1989	2662139
review of cardiovascular findings in humans treated with minoxidil.	minoxidil is an antihypertensive vasodilator with no known capability of inducing human cardiovascular pathology; however, the compound does cause a late, canine-specific lesion of the right atrium and acute right atrial and ventricular papillary muscle changes. in all the autopsy cases of minoxidil-treated humans to date, 9 cases were reported with significant right atrial or other cardiac pathology and were carefully reviewed for similarity to either the early or late canine lesion. three new ...	1989	2665032
vibrio vulnificus infection in hawaii.	a life-threatening vibrio vulnificus infection occurred in a 52-year-old korean woman with hepatic cirrhosis. four days after ingesting raw crab, the patient presented to the hospital with nausea, vomiting, fever, hypotension, and hemorrhagic blistering of the left foot. vibrio vulnificus was recovered from both her blood and a foot wound.	1989	2666323
infectious and toxic syndromes from fish and shellfish consumption. a review.	primary care physicians care for large numbers of patients presenting with "food poisoning" or gastroenteritis. when a patient who presents with acute gastrointestinal illness, especially in conjunction with neurologic or cutaneous symptoms, is evaluated, the history should focus on past seafood consumption (particularly raw or undercooked seafood). the infectious syndromes are generally self-limited and respond to supportive care; exceptions are those caused by vibrio cholerae and vibrio vulnif ...	1989	2669661
a new procedure for the targeted inactivation of essential bacterial genes.	a new method is described for the exchange of a plasmid encoded mutant gene with its chromosomal counterpart. the method is based on positive selection and is applicable for the exchange of essential genes. the main features of the method are: (1) cloning of an antibiotic resistance marker (without its promoter) downstream of the cloned target gene, thus forming an artificial operon; (2) inactivating the target gene (and consequently also the antibiotic resistance gene) by inserting a strong tra ...	1989	2674660
a new procedure for the targeted inactivation of essential bacterial genes.	a new method is described for the exchange of a plasmid encoded mutant gene with its chromosomal counterpart. the method is based on positive selection and is applicable for the exchange of essential genes. the main features of the method are: (1) cloning of an antibiotic resistance marker (without its promoter) downstream of the cloned target gene, thus forming an artificial operon; (2) inactivating the target gene (and consequently also the antibiotic resistance gene) by inserting a strong tra ...	1989	2674660
a biotin-streptavidin amplified enzymeimmunoassay for 13,14-dihydro-15-keto-pgf2 alpha.	as an alternative for radioimmunoassays a new enzyme immunoassay (eia) for the determination of 13,14-dihydro-15-keto pgf2 alpha (pgfm) has been developed. biocytin was linked to pgfm by the n-hydroxysuccinimide method and the product (biocytinyl-pgfm) purified by reversed phase column chromatography. biocytinyl-pgfm was used in the eia as a bridge between the immobilized pgfm-antibody and streptavidin-peroxidase. the absolute sensitivity of the assay was about 160 amol (92% rel. binding) and re ...	1989	2675197
establishment and characterization of human glioblastoma cell lines in vitro and their xenografts in nude mice.	human gliomas were grown as permanent tissue cultures and xenografts in nude mice. growth kinetics, immunohistology and karyotypes were established. one tumor showed a distinct change in its karyotype after only two passages in nude mice; by contrast, no change of immunophenotype occurred during and after in vivo passaging. treatment of this glioma as xenograft in nude mice revealed a high sensitivity towards single agent treatment with bcnu. in view of the cytologic aberrations, however, this r ...	1989	2677876
digit-muscle responses evoked from multiple intracortical foci in monkey precentral motor cortex.	1. the precentral motor cortex, including the anterior bank of the central sulcus of monkey (macaca fuscata), was systemically penetrated with microelectrodes to determine the spatial organization of the microexcitable cortical elements that can produce responses in digit muscles. 2. at 200-microns intervals on each electrode track, low-current intracortical microstimuli were delivered and the muscle responses evoked from four digit muscles were recorded. the responses, obtained with 5, 8, 15, a ...	1989	2681562
acute gluteal abscesses: injectable chloroquine as a cause.	one hundred and seven patients admitted to the casualty department with acute gluteal abscesses and surgically drained were studied. in 91 patients (85%) the abscess was a result of an intramuscular injection, of whom 71 patients (66%) had the abscess following a chloroquine injection (antimalarial). of those patients with an abscess following an intramuscular injection, in 64 patients (70%) a disposable syringe was used for injection of the drug and in 18% a boiled disposable was used. the inje ...	1989	2681814
phosphoroselenoate oligodeoxynucleotides: synthesis, physico-chemical characterization, anti-sense inhibitory properties and anti-hiv activity.	oligodeoxynucleotides with a phosphorus atom in which one of the non-bridging oxygen atoms is substituted by selenium were prepared and investigated with respect to their antisense properties. a general synthesis of phosphoroselenoate analogs of oligonucleotides is described using potassium selenocyanate as the selenium donor. the compounds, characterized by 31p nmr, were shown to decompose to phosphate with a half-life of ca. 30 days. melting temperatures of duplexes between poly(ra) or poly(ri ...	1989	2682524
the common src homology region 2 domain of cytoplasmic signaling proteins is a positive effector of v-fps tyrosine kinase function.	a conserved noncatalytic domain sh2 (for src homology region 2) is located immediately n terminal to the kinase domains of all cytoplasmic protein-tyrosine kinases. we found that the wild-type v-fps sh2 domain stimulated the enzymatic activity of the adjacent kinase domain 10-fold and functioned as a powerful positive effector of catalytic and transforming activities within the v-fps oncoprotein (p130gag-fps). partial proteolysis of p130gag-fps and supporting genetic data indicated that the v-fp ...	1989	2685548
the common src homology region 2 domain of cytoplasmic signaling proteins is a positive effector of v-fps tyrosine kinase function.	a conserved noncatalytic domain sh2 (for src homology region 2) is located immediately n terminal to the kinase domains of all cytoplasmic protein-tyrosine kinases. we found that the wild-type v-fps sh2 domain stimulated the enzymatic activity of the adjacent kinase domain 10-fold and functioned as a powerful positive effector of catalytic and transforming activities within the v-fps oncoprotein (p130gag-fps). partial proteolysis of p130gag-fps and supporting genetic data indicated that the v-fp ...	1989	2685548
[principle, method, and application for the production of peptide hormones using genetic engineering].		1989	2691727
falciparum malaria--present day problems. an experience with 425 cases.	clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ...	1989	2693436
vibrio vulnificus infection.	we report 3 cases of vibrio vulnificus infections from taiwan. patient 1, who manifested symptoms of primary septicemia, died after 2 days. patient 2, who had a wound infection and signs and symptoms of sepsis but negative blood cultures, responded to tobramycin and chloramphenicol plus surgical debridement, and recovered after 26 days of hospitalization. patient 3 had secondary septicemia originating from a wound inflicted by a shrimp. originally, the patient seemed to respond to ceftazidime an ...	1989	2694352
vibrio vulnificus septicaemia presenting as spontaneous necrotising cellulitis in a woman with hepatic cirrhosis.	vibrio vulnificus is a virulent marine organism commonly found in hong kong coastal waters which contaminates local sea-food. it may produce a primary septicaemia, often associated with secondary skin lesions, following ingestion of raw shell fish. we report a rapidly fatal case of primary v. vulnificus septicaemia in a 50-year-old housewife with post-hepatitic cirrhosis presenting as spontaneous necrotising cellulitis of the legs. v. vulnificus infection should be considered in patients with a ...	1989	2694353
parallels between neurasthenia and premenstrual syndrome.	neurasthenia and premenstrual syndrome became medical diseases because of the historical recognition of menstruation as a medical disease. both the nineteenth and twentieth century cultural views of women were important in the establishment of menstruation, neurasthenia and premenstrual syndrome as medical conditions. uncertainty of diagnosis with ever expanding diagnostic criteria, therapy undertaken without an adequate physiological basis, and often adverse effects from therapy, were character ...	1989	2694626
mechanism of lhrh-stimulated steroidogenesis in rat leydig cells: lipoxygenase products of arachidonic acid may not be involved.	luteinizing hormone releasing hormone agonist, [(imbzl)-dhis6,pro9,net]-lhrh (lhrh-a), caused a two to threefold increase in in vitro testosterone (t) secretion by rat leydig cells. this lhrh-a-induced t secretion was completely blocked by quinacrine and chloroquine, inhibitors of phospholipase a2. addition of phospholipase a2, however, was ineffective in stimulating basal or lhrh-a-induced t secretion. phospholipase c, on the other hand, significantly stimulated both basal and lhrh-a-induced t ...	1989	2695506
membrane fatty acid and virulence changes in the viable but nonculturable state of vibrio vulnificus.	the nonculturable state of vibrio vulnificus and, for comparison, that of escherichia coli were studied in artificial-seawater microcosms at 5 degrees c. total cell counts were monitored by acridine orange epifluorescence, metabolic activity by direct viable counts, and culturability by plate counts on selective and nonselective media. whereas total counts remained constant, plate counts of v. vulnificus suggested nonculturability by day 24. in contrast, direct viable counts indicated significan ...	1989	2696428
vibrio vulnificus sepsis after eating raw oysters.	vibrio vulnificus, a marine vibrio found in coastal waters of the united states, may contaminate certain seafoods, particularly raw oysters. patients with underlying liver disease are particularly susceptible to severe illness. unexplained febrile diseases in patients who have eaten raw oysters may be caused by vibrio vulnificus. a fatal and a nonfatal case are reported in two such patients. these patients are the first two reported cases of vibrio vulnificus infection in kentucky.	1989	2723530
characterization and purification of h1tf2, a novel ccaat-binding protein that interacts with a histone h1 subtype-specific consensus element.	definition of mechanisms regulating human histone h1 gene transcription during the cell cycle requires the isolation and biochemical characterization of protein factors which interact with specific promoter elements. two distinct binding activities have been identified in nuclear extracts from hela cells and mapped within a 180-base-pair (bp) region of a cell cycle-regulated h1 gene promoter. h1tf1 bound to an h1-specific a + c-rich sequence (ac box), 100 bp upstream of the cap site; h1tf2 inter ...	1989	2725515
primary structures of sm31/32 diagnostic proteins of schistosoma mansoni and their identification as proteases.	we have constructed cdna clones containing the complete nucleotide sequences coding for two highly antigenic schistosoma mansoni adult worm proteins, sm31 and sm32. the predicted amino acid sequence of sm31 shows significant homology to mouse, rat and human cathepsin b. the nucleotide sequence of sm32 is identical to that reported by others for s. mansoni "haemoglobinase'. the different nucleotide sequences demonstrate the existence of two different proteolytic enzymes, both of which are synthes ...	1989	2725581
vibrio sepsis in a cirrhotic patient.	vibrio vulnificus should be suspected in any soft tissue infection occurring after exposure to the marine environment. early recognition followed by appropriate antibiotic therapy and debridement of involved tissue are essential features in the treatment of these infections.	1989	2734646
ventricular arrhythmias and local electrograms after chronic regional denervation of the ischemic area in the pig heart.	cardiac denervation has been proved to reduce the incidence of coronary occlusion arrhythmias in digs, but the effect of limiting the extent of sympathectomy to the ischemic area, particularly in hearts with sparse coronary collateral circulation, as in the human heart, needs further investigation. ventricular arrhythmias and changes in epicardial direct current electrograms induced during acute left anterior descending coronary artery occlusion were recorded in 14 pigs subjected to regional den ...	1989	2738265
[complexing of bovine serum albumin with phosphatidyl choline liposomes].	thermodynamic parameters of the bovine serum albumin interaction with phosphatidyl choline liposomes have been determined. it is shown that the bovine serum albumin is adsorbed on the liposomal surface without change of the globule conformation, the polar interactions making dominant contribution in immobilization. the complexing is characterized by a binding constant and a great number of binding sites.	1989	2749908
attempted passive transfer of a serum factor in chronic urticaria from human to monkey.		1989	2757947
2-bromo- and 16-bromo-estrogens and related compounds: potential inhibitors for both estradiol 2- and 16 alpha-hydroxylases in rat liver microsomes.	kinetic studies of inhibition of estradiol 2- and 16 alpha-hydroxylase activities in male rat liver microsomes with 2-bromoestrogens, 4-bromo-estrone (4-bre1), 16 alpha- and 16 beta-bromoestrones and 16 beta-acetylthioestrone (16-acse1) were carried out. 2-bromoestrogens and 4-bre1 nonspecifically blocked the two enzyme activities in a competitive manner, and 2-bromo-estradiol (2-bre2) was the most potent inhibitor for the two hydroxylases among the 2- and 4-bromo steroids. kinetic data, the app ...	1989	2761259
transcriptional start site in the mouse cyp1a1 (cytochrome p1450) gene.	two transcriptional initiation sites have been described by different laboratories for the mouse cyp1a1 (cytochrome p1450) gene: start site ti, at the beginning of the nontranslated 87-bp first exon, and start site tii, 635 bp downstream from site ti within the 2,380-bp first intron. site ti was characterized in normal c57bl/6 mouse liver, whereas site tii was described in a high activity variant line (hav cells) derived from the mouse hepatoma hepa-1c1c7 wild type (wt) established cell line. it ...	1989	2766934
[cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control].	fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ...	1989	2772364
phagocytosis by guinea pig polymorphonuclear leukocytes of liposomes stabilized with polysaccharides.	hemolysate-loaded liposomes were prepared with their surface coated by polysaccharides, carboxymethylchitin, carboxymethylcellulose and carboxymethylchitosan. phagocytosis by guinea pig polymorphonuclear leukocytes of these coated liposomes was measured in view of electrostatic interactions between the cells and the liposomes. this view revealed to hold at least in quite an early stage of phagocytosis, but some specific interactions were assumed to exist between the cells and the liposomes. n-ac ...	1989	2775873
suppression of antibody responses to the acetylcholine receptor by natural antibodies.	some relationships between "natural" physiological autoreactivity and antibody responses against the acetylcholine receptor (achr) were investigated. collections of unselected monoclonal antibodies were derived from nonmanipulated newborn mice of susceptible (balb.b) and resistant (balb/c) mouse strains, and screened for reactivity with heterologous achr. five in 200 balb/c but none in 200 balb.b clones were found reactive. four of these anti-achr antibodies were analyzed for connectivity to nin ...	1989	2776827
virulence of vibrio vulnificus strains isolated from seafoods.	a study was carried out on the relation between virulence of v. vulnificus assessed by ld50 in mice and other markers. v. vulnificus strains isolated from seafoods had ld50 ranging from 10(3)-10(7) for mice. strains showing ld50 10(6) or less were generally haemolytic and settled after boiling. resistance to bactericidal action of serum and ability to take up congo red was shown by all the strains.	1989	2777343
human eosinophils express cd4 protein and bind human immunodeficiency virus 1 gp120.	the cd4 glycoprotein, expressed on leukocytes belonging to subsets of t lymphocytes and to cells of monocyte/macrophage lineage, participates in the functioning of t cells and serves as a receptor for hiv-1 and hiv-2. human eosinophils, a class of granulocytic leukocytes, have been found to express cd4. with anti-cd4 mabs cd4 was demonstrable on eosinophils from both normal and eosinophilic donors. eosinophils synthesized a 55-kd cd4 polypeptide immunoprecipitable with two anti-cd4 mabs. eosinop ...	1989	2783333
effect of vitamin d metabolites on calcitriol metabolism in experimental renal failure.	previous studies from our laboratory have demonstrated that metabolic clearance rate (mcr) of calcitriol is decreased in experimental renal failure. in this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin d3 (25(oh)d3) and 24,25-dihydroxyvitamin d3 (24,25(oh)2d3) on the mcr of calcitriol in renal failure produced in rats by partial nephrectomy. the mcr of calcitriol in these rats with renal failure was significantly lower than in control rats with sham operations. plasma con ...	1989	2789310
[intestinal microbial ecology and its modulation under the influence of immunodepressants].	oral administration of immunodepressants such as imuran (purine analog) and batriden (gossypol derivative) for 3 months led to development of dysbacterioses in various sections of the rat gastrointestinal tract. the dysbacterioses differed in their levels and the pattern of the recovery process. as compared to batriden, imuran in a dose of 30 mg/kg body weight administered at the early observation periods (days 7, 14 and 30) induced more marked disorders in the intestine microecology. the imuran ...	1989	2802878
role of carboxylesterase in soman, sarin and tabun poisoning in rats.	tocp (triorthocresyl phosphate) inhibits carboxylesterase (carbe) activity in rat plasma and liver and significantly increases soman, sarin and tabun toxicity. application of these agents after tocp caused strong additional inhibition of carbe and cholinesterases (che) in rat red blood cells, plasma, liver, brain, diaphragm and intercostal muscle. after phenobarbital pretreatment, which induced carbe activity in plasma and liver by 80% and that of plasma che by 33%, acute toxicity of soman and t ...	1989	2813290
the dermatopathologist and human immunodeficiency virus infection.		1989	2817921
an experimental study of susceptibility to infection after hemorrhagic shock.	hemorrhagic shock has been associated with an increased risk of infection after injury. the immediate and long term effects of hemorrhagic shock without tissue injury on the susceptibility of an animal to infection and the efficacy of antibiotic prophylaxis to prevent infection in this setting were examined. sprague-dawley rats were subjected to hemorrhagic shock (ld15) by bleeding to a mean arterial pressure of 45 millimeters of mercury for 45 minutes and were resuscitated with shed blood and n ...	1989	2911791
human milk feeding enhances the urinary excretion of immunologic factors in low birth weight infants.	the effects of fortified human milk feedings on the urinary excretion of lactoferrin, lysozyme, secretory component, iga, and secretory iga antibodies to escherichia coli o antigens were investigated in very low birth wt infants. infants were maintained on either a human milk or a cow's milk preparation. the amounts of each immune factor that were ingested and excreted were quantified during balance studies conducted at 2.5 and 5 wk of age. serum levels of these immune factors were similar in bo ...	1989	2919134
degradation of type ix collagen by matrix metalloproteinase 3 (stromelysin) from human rheumatoid synovial cells.	the degradation of type ix collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (mmps) purified from the culture medium of rheumatoid synovial cells. neither mmp-1 (collagenase) nor mmp-2 (so-called 'gelatinase') could digest type ix collagen, but mmp-3 (stromelysin) readily degraded it into smaller fragments. this suggests that mmp-3 may be responsible for the pathological degradation and/or normal turnover of type ix collage ...	1989	2920840
vibrio vulnificus corneal ulcer. case reports.		1989	2923553
vibrio vulnificus infections.		1989	18750602
basic training and the part i examination.		1989	267474
specificity and sensitivity of technetium-99m pyrophosphate myocardial imaging in acute myocardial infarction.		1989	203178
quality touching to communicate caring.		1988	259270
new selective and differential medium for vibrio cholerae and vibrio vulnificus.	[this corrects the article on p. 2263 in vol. 53.].	1988	16347534
a multicentre open clinical trial of teicoplanin in infections caused by gram-positive bacteria.	a multicentre open trial of teicoplanin in 81 centres in nine european countries included 1431 cases: 531 female, 900 male; mean age 49.4 years, range 1-93 years. these were hospitalized patients most of whom had infections caused by staphylococcus aureus (816 isolates). of a total of 1427 gram-positive pathogens 280 (19.6%) were methicillin resistant. there were 536 skin and soft tissue infections, 263 septicaemias, 135 lower respiratory tract infections, 179 joint and bone infections and 83 en ...	1988	2965133
cns-induced natriuresis during dopamine receptor blockade. further support for the existence of, at least, two separate natriuretic hormonal systems.	intracerebroventricular (icv) stimulation with hypertonic sodium chloride solutions has previously shown indications to stimulate the release of a blood-borne natriuretic factor. sodium excretion in this situation increases in the order of 10-20 times. acute isotonic volume expansion has shown to be a potent stimulus for endogenous release of the atrial natriuretic factor (anf). it has also been demonstrated that the natriuretic response to both volume expansion and exogenously applied anf can b ...	1988	2976234
mononuclear cell infiltration in central portions of human astrocytomas.	the mononuclear cell infiltration in the central portion of human low- and high-grade astrocytomas has been examined using monoclonal antibodies to cell surface differentiation antigens. there was no significant difference between the number of t cells, b cells, or macrophages. the okt8+ (suppressor/cytotoxic) t cell population was the major lymphocyte subset in the infiltrates. there was, however, indirect evidence for a proportion of the total okt8+ cells being natural killer cells. overall, t ...	1988	2830373
mononuclear cell infiltration in central portions of human astrocytomas.	the mononuclear cell infiltration in the central portion of human low- and high-grade astrocytomas has been examined using monoclonal antibodies to cell surface differentiation antigens. there was no significant difference between the number of t cells, b cells, or macrophages. the okt8+ (suppressor/cytotoxic) t cell population was the major lymphocyte subset in the infiltrates. there was, however, indirect evidence for a proportion of the total okt8+ cells being natural killer cells. overall, t ...	1988	2830373
cilia-associated respiratory (car) bacillus infection of obese mice.	cilia-associated respiratory (car) bacillus was identified in respiratory tract lesions of obese mice dying of chronic respiratory disease. neither mycoplasma pulmonis nor pathogenic bacteria were isolated from cultures of the lesions at necropsy, but there was serologic and histologic evidence of respiratory virus infection. cranial-ventral areas of lung were firm and demarcated from unaffected lung at gross examination, and representative tissue sank in water. microscopically, there was suppur ...	1988	2830699
cilia-associated respiratory (car) bacillus infection of obese mice.	cilia-associated respiratory (car) bacillus was identified in respiratory tract lesions of obese mice dying of chronic respiratory disease. neither mycoplasma pulmonis nor pathogenic bacteria were isolated from cultures of the lesions at necropsy, but there was serologic and histologic evidence of respiratory virus infection. cranial-ventral areas of lung were firm and demarcated from unaffected lung at gross examination, and representative tissue sank in water. microscopically, there was suppur ...	1988	2830699
cloning and expression of the vibrio cholerae neuraminidase gene nanh in escherichia coli.	a cosmid gene bank of vibrio cholerae 395, classical ogawa, was screened in escherichia coli hb101 for expression of the vibrio neuraminidase (nanase) gene nanh (n-acylneuraminate glycohydrolase). positive clones were identified by their ability to cleave the fluorogenic nanase substrate 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid. seven nanase-positive clones were detected after screening 683 cosmid isolates with a rapid, qualitative plate assay method. the nanh gene was subcloned ...	1988	2832365
cloning and expression of the vibrio cholerae neuraminidase gene nanh in escherichia coli.	a cosmid gene bank of vibrio cholerae 395, classical ogawa, was screened in escherichia coli hb101 for expression of the vibrio neuraminidase (nanase) gene nanh (n-acylneuraminate glycohydrolase). positive clones were identified by their ability to cleave the fluorogenic nanase substrate 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid. seven nanase-positive clones were detected after screening 683 cosmid isolates with a rapid, qualitative plate assay method. the nanh gene was subcloned ...	1988	2832365
propranolol enhances in vitro interleukin 2 receptor expression on human lymphocytes.	interleukin 2 receptor (il-2r) expression was assessed, using anti-tac monoclonal antibody, after incubation of human lymphocytes with the beta-adrenoceptor antagonist propranolol (pro). one-hour incubation of mononuclear cells (mnc) with pro resulted in an increase of tac positive cells. the greatest rise was observed with a pro concentration of 10(-6) m (29% ring-stained cells compared with 0% in the control group). cells cultured for 3 days with pro showed a lower proportion of tac positive c ...	1988	2836304
mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis.	in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ...	1988	2838023
transcriptional regulation of the gene encoding the major surfactant protein (sp-a) in rabbit fetal lung.	the expression of the major protein of rabbit pulmonary surfactant (sp-a), a glycoprotein of mr congruent to 29,000-36,000, is regulated during development and by hormones. in the present study, utilizing a cdna insert complementary to mrna coding for sp-a and nuclear transcription elongation assays, we have investigated the developmental and hormonal regulation of transcription of the sp-a gene in rabbit fetal lung tissue. the relative rates of transcription of sp-a mrna increased as a function ...	1988	2848814
[the entero-insular axis: the new incretin candidate glucagon-like peptide-1(7-36)amide (glp-1(7-36))amide].	the functional connection between the gut and pancreatic islets is described by the term "enteroinsular axis". a humoral factor of the gut that might enhance the glucose-induced secretion of insulin is named "incretin". for many years glucose-dependent insulin-releasing polypeptide (gip) was the strongest incretin candidate. however, recent evidence suggests that glucagon-like peptide-1(7-36)amide represents a more potent physiological incretin. the sequence of glp-1 is identical in various mamm ...	1988	2849249
factors influencing the severity of escherichia coli and avian adenovirus group ii infections in chickens.	chickens from lines selectively bred for either a high (hh) or low (ll) antibody response to sheep erythrocytes were challenged intravenously with avian adenovirus group ii (aa). spleen size was determined 6 days later. in some experiments the responses of chickens to aa and escherichia coli infections were compared. the level of corticosterone in the feed (15 mg/kg) which resulted in the lowest incidence of pericarditis in response to e. coli resulted in the greatest incidence of large spleens ...	1988	2849405
suppression of sv40-promoted gene expression by differentiation of preadipose cells.	when a plasmid bearing the chloramphenicol acetyltransferase (cat) gene under the control of an sv40 early promoter is introduced into preadipose or adipose cells of line 3t3-f442a, the promoter directs high levels of transient expression of cat. however, when the plasmid is introduced into preadipose cells and the cells are then allowed to differentiate into adipose cells, the expression of the cat gene is suppressed. in this process, the plasmid is not changed detectably in amount, topology, o ...	1988	2849579
[changes in the cyclic nucleotide system of t-lymphocytes in experimental tuberculosis in animals].		1988	2851783
a murine monoclonal antibody raised against human non-small cell carcinoma of the lung.	murine monoclonal antibodies (mabs) reactive with human non-small cell carcinoma of the lung (nsccl) were produced following immunization with a membrane preparation of calu-3, a human adenocarcinoma of the lung cell line grown in nude mice. positive hybrids unreactive with normal liver membranes and human peripheral white blood cells were selected for further testing. one mab (rs5-4h6) recognized an antigen expressed in a variety of lung and other carcinoma cell lines as detected by flow cytome ...	1988	2853133
new differentiation antigens associated with the growth and maturation of b lymphocytes.	monoclonal antibodies (mab) nda3 and nda4 were generated by hyperimmunizing mice with an alloreactive human t cell clone and fusing the splenocytes with the ns1 myeloma. immunofluorescence studies indicated that these mab react with activated, but not with resting t and b lymphocytes or with other types of cells. immunoprecipitation studies with 125-i-labeled extracts from t and b cell lines demonstrated that the m.w. of the antigen recognized by mab nda3 is 36,000 and that of nda4 is 46,000. st ...	1988	2447175
importance of pulmonary blood volume and aortic blood pressure in regulation of left ventricular function.		1988	2900297
inhibitory effect of glycyrrhetinic acid derivatives on arachidonic acid-induced mouse ear oedema.	the inhibitory effects of glycyrrhetinic acid and its derivatives were examined on arachidonic acid (aa)-induced ear oedema in mice. of the compounds, dihemiphthalate derivatives of 18 beta-olean-12-ene-3 beta, 30-diol (iid, iid'), 18 beta-olean-9(11)12-diene-3 beta, 30-diol (iiia, iiia') and olean-11, 13(18)-diene-3 beta, 30-diol (iva, iva') showed a strong inhibition of ear oedema on both tropical (id50, 1.9, 2.8 and 1.7 mg/ear, respectively) and oral (id50, 90, 130 and 88 mg kg-1, respectivel ...	1988	2900308
neuronal interactions between neuropeptide y (npy) and catecholaminergic systems in the rat arcuate nucleus as shown by dual immunocytochemistry.	several recent studies have suggested interactions between catecholamine (ca) and neuropeptide y (npy) neuronal systems in the rat brain. in order to obtain morphological evidence for such ca/npy interactions in the arcuate nucleus, we have used a double immunostaining procedure using an anti-tyrosine hydroxylase (th) antiserum as a marker for catecholamine neurons and an anti-npy antiserum. this double staining, where the first staining is silver-gold intensified, was detectable at both light a ...	1988	2901738
changes in the metabolic profile of the equine gluteus medius as a function of sampling depth.	1. cross sections from the middle of the gluteus medius were removed from 10 adult horses and used to evaluate changes in histochemically determined muscle fiber type and biochemically determined metabolic enzyme activities as a function of sample depth. 2. muscle fiber types determined using histochemical methods for myosin atpase (ph 9.4) and succinic dehydrogenase (sdh) activity indicated percent fast-twitch glycolytic (fg) muscle fibers decreased and slow-twitch oxidative (so) fibers increas ...	1988	2907449
eukaryote membrane genetics: the fo sector of mitochondrial atp synthase.		1988	2907692
structural and functional studies of the effects of sympathetic nerve stimulation on rabbit submandibular salivary glands.	continuous sympathetic stimulation at 8-10 hz caused intense vasoconstriction in the gland, so stimulation was generally given in an interrupted pattern to minimize this detrimental effect on secretion. only a small increase in fluid secretion occurred; it became thick and tended to block the cannula; therefore in later experiments the main duct was not cannulated. after sympathetic stimulation there was substantial degranulation of acinar cells. however, as this was accompanied by little moveme ...	1988	3165262