Publications

[two case reports of septic shock due to vibrio vulnificus with liver cirrhosis].	we have recently experienced a case of vibrio vulnificus septicemia which occurred in a patient with hepatic cirrhosis, and as we were able to give early antibiotic treatment, the patient survived. we would like to report this case here together with another case experienced 2 years ago. case 1 was a 58-year-old male who was attending our hospital as an outpatient for hepatic cirrhosis. at 5:30 pm on august 8, 1987, he consumed abalone and giant clam and at 9 pm complained of high fever with sha ...	1989	2501432
an experimental study of susceptibility to infection after hemorrhagic shock.	hemorrhagic shock has been associated with an increased risk of infection after injury. the immediate and long term effects of hemorrhagic shock without tissue injury on the susceptibility of an animal to infection and the efficacy of antibiotic prophylaxis to prevent infection in this setting were examined. sprague-dawley rats were subjected to hemorrhagic shock (ld15) by bleeding to a mean arterial pressure of 45 millimeters of mercury for 45 minutes and were resuscitated with shed blood and n ...	1989	2911791
comments on radiation-induced g2 arrest in mouse zygotes.		1989	2564879
degradation of type ix collagen by matrix metalloproteinase 3 (stromelysin) from human rheumatoid synovial cells.	the degradation of type ix collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (mmps) purified from the culture medium of rheumatoid synovial cells. neither mmp-1 (collagenase) nor mmp-2 (so-called 'gelatinase') could digest type ix collagen, but mmp-3 (stromelysin) readily degraded it into smaller fragments. this suggests that mmp-3 may be responsible for the pathological degradation and/or normal turnover of type ix collage ...	1989	2920840
vibrio vulnificus corneal ulcer. case reports.		1989	2923553
inhibitory effect of alpha 2-macroglobulin on vibrio vulnificus protease.	vibrio vulnificus, an etiologic agent of wound infections and septicemia in humans, elaborates a metalloprotease which is known to be an important virulence factor of the vibrio. the proteolytic activity of v. vulnificus metalloprotease (vvp) toward casein and elastin was inhibited by alpha 2-macroglobulin (alpha 2 m) at the molar ratio of 1:1, although partial activity was maintained. permeability-enhancing and hemorrhagic activities were also inhibited, but the peptidase activity toward z-gly- ...	1989	2478526
a simple colorimetric assay method for pyrophosphate in the presence of a 1000-fold excess of orthophosphate: application of the method to the study of pyrophosphate metabolism in mitochondria.	a sensitive colorimetric method for the assay of inorganic pyrophosphate with excess of orthophosphate is described. the principle of this method lies in the formation of phosphomolybdate and ppi-molybdate complexes with subsequent extraction of the phosphomolybdate complex by organic solvents and reduction of the ppi-molybdate complex by dithiothreitol and eikonogen. the sensitivity of the method was from 5 to 120 nmol of ppi in a 2.0-ml sample.	1989	2545112
septicaemia due to vibrio vulnificus.		1989	2617638
[rational treatment of necrotizing external otitis].	advances in the diagnosis and treatment of necrotizing external otitis (neo) during the past 20 years improved substantially the prognosis of this disease. the use of hyperbaric oxygen therapy greatly potentiates the effect of newly developed highly effective antibiotics. radioisotope examination of the petrous bones facilitates the diagnosis and indicates the time to terminate treatment. medicamentous treatment is preferable to surgical treatment, which is reserved for exceptional very advanced ...	1989	2631997
[rational treatment of necrotizing external otitis].	advances in the diagnosis and treatment of necrotizing external otitis (neo) during the past 20 years improved substantially the prognosis of this disease. the use of hyperbaric oxygen therapy greatly potentiates the effect of newly developed highly effective antibiotics. radioisotope examination of the petrous bones facilitates the diagnosis and indicates the time to terminate treatment. medicamentous treatment is preferable to surgical treatment, which is reserved for exceptional very advanced ...	1989	2631997
vibrio vulnificus infection.	we report 3 cases of vibrio vulnificus infections from taiwan. patient 1, who manifested symptoms of primary septicemia, died after 2 days. patient 2, who had a wound infection and signs and symptoms of sepsis but negative blood cultures, responded to tobramycin and chloramphenicol plus surgical debridement, and recovered after 26 days of hospitalization. patient 3 had secondary septicemia originating from a wound inflicted by a shrimp. originally, the patient seemed to respond to ceftazidime an ...	1989	2694352
vibrio vulnificus septicaemia presenting as spontaneous necrotising cellulitis in a woman with hepatic cirrhosis.	vibrio vulnificus is a virulent marine organism commonly found in hong kong coastal waters which contaminates local sea-food. it may produce a primary septicaemia, often associated with secondary skin lesions, following ingestion of raw shell fish. we report a rapidly fatal case of primary v. vulnificus septicaemia in a 50-year-old housewife with post-hepatitic cirrhosis presenting as spontaneous necrotising cellulitis of the legs. v. vulnificus infection should be considered in patients with a ...	1989	2694353
detection of vibrio vulnificus cytolysin in v. vulnificus-infected mice.	enzyme-linked immunosorbent assays using polyclonal and monoclonal antibodies specific for v. vulnificus cytolysin detected the toxin in an extract of skin lesions and in serum from mice showing local and systemic v. vulnificus disease after subcutaneous injection of the bacterium. the cytolysin also was detected in skin lesions by an indirect immunofluorescence procedure using polyclonal and monoclonal antibodies. our findings provide direct evidence that the cytolysin is produced in vivo durin ...	1989	2658204
the common src homology region 2 domain of cytoplasmic signaling proteins is a positive effector of v-fps tyrosine kinase function.	a conserved noncatalytic domain sh2 (for src homology region 2) is located immediately n terminal to the kinase domains of all cytoplasmic protein-tyrosine kinases. we found that the wild-type v-fps sh2 domain stimulated the enzymatic activity of the adjacent kinase domain 10-fold and functioned as a powerful positive effector of catalytic and transforming activities within the v-fps oncoprotein (p130gag-fps). partial proteolysis of p130gag-fps and supporting genetic data indicated that the v-fp ...	1989	2685548
vibrio vulnificus infection in hawaii.	a life-threatening vibrio vulnificus infection occurred in a 52-year-old korean woman with hepatic cirrhosis. four days after ingesting raw crab, the patient presented to the hospital with nausea, vomiting, fever, hypotension, and hemorrhagic blistering of the left foot. vibrio vulnificus was recovered from both her blood and a foot wound.	1989	2666323
infectious and toxic syndromes from fish and shellfish consumption. a review.	primary care physicians care for large numbers of patients presenting with "food poisoning" or gastroenteritis. when a patient who presents with acute gastrointestinal illness, especially in conjunction with neurologic or cutaneous symptoms, is evaluated, the history should focus on past seafood consumption (particularly raw or undercooked seafood). the infectious syndromes are generally self-limited and respond to supportive care; exceptions are those caused by vibrio cholerae and vibrio vulnif ...	1989	2669661
a new procedure for the targeted inactivation of essential bacterial genes.	a new method is described for the exchange of a plasmid encoded mutant gene with its chromosomal counterpart. the method is based on positive selection and is applicable for the exchange of essential genes. the main features of the method are: (1) cloning of an antibiotic resistance marker (without its promoter) downstream of the cloned target gene, thus forming an artificial operon; (2) inactivating the target gene (and consequently also the antibiotic resistance gene) by inserting a strong tra ...	1989	2674660
a new procedure for the targeted inactivation of essential bacterial genes.	a new method is described for the exchange of a plasmid encoded mutant gene with its chromosomal counterpart. the method is based on positive selection and is applicable for the exchange of essential genes. the main features of the method are: (1) cloning of an antibiotic resistance marker (without its promoter) downstream of the cloned target gene, thus forming an artificial operon; (2) inactivating the target gene (and consequently also the antibiotic resistance gene) by inserting a strong tra ...	1989	2674660
a biotin-streptavidin amplified enzymeimmunoassay for 13,14-dihydro-15-keto-pgf2 alpha.	as an alternative for radioimmunoassays a new enzyme immunoassay (eia) for the determination of 13,14-dihydro-15-keto pgf2 alpha (pgfm) has been developed. biocytin was linked to pgfm by the n-hydroxysuccinimide method and the product (biocytinyl-pgfm) purified by reversed phase column chromatography. biocytinyl-pgfm was used in the eia as a bridge between the immobilized pgfm-antibody and streptavidin-peroxidase. the absolute sensitivity of the assay was about 160 amol (92% rel. binding) and re ...	1989	2675197
establishment and characterization of human glioblastoma cell lines in vitro and their xenografts in nude mice.	human gliomas were grown as permanent tissue cultures and xenografts in nude mice. growth kinetics, immunohistology and karyotypes were established. one tumor showed a distinct change in its karyotype after only two passages in nude mice; by contrast, no change of immunophenotype occurred during and after in vivo passaging. treatment of this glioma as xenograft in nude mice revealed a high sensitivity towards single agent treatment with bcnu. in view of the cytologic aberrations, however, this r ...	1989	2677876
digit-muscle responses evoked from multiple intracortical foci in monkey precentral motor cortex.	1. the precentral motor cortex, including the anterior bank of the central sulcus of monkey (macaca fuscata), was systemically penetrated with microelectrodes to determine the spatial organization of the microexcitable cortical elements that can produce responses in digit muscles. 2. at 200-microns intervals on each electrode track, low-current intracortical microstimuli were delivered and the muscle responses evoked from four digit muscles were recorded. the responses, obtained with 5, 8, 15, a ...	1989	2681562
acute gluteal abscesses: injectable chloroquine as a cause.	one hundred and seven patients admitted to the casualty department with acute gluteal abscesses and surgically drained were studied. in 91 patients (85%) the abscess was a result of an intramuscular injection, of whom 71 patients (66%) had the abscess following a chloroquine injection (antimalarial). of those patients with an abscess following an intramuscular injection, in 64 patients (70%) a disposable syringe was used for injection of the drug and in 18% a boiled disposable was used. the inje ...	1989	2681814
phosphoroselenoate oligodeoxynucleotides: synthesis, physico-chemical characterization, anti-sense inhibitory properties and anti-hiv activity.	oligodeoxynucleotides with a phosphorus atom in which one of the non-bridging oxygen atoms is substituted by selenium were prepared and investigated with respect to their antisense properties. a general synthesis of phosphoroselenoate analogs of oligonucleotides is described using potassium selenocyanate as the selenium donor. the compounds, characterized by 31p nmr, were shown to decompose to phosphate with a half-life of ca. 30 days. melting temperatures of duplexes between poly(ra) or poly(ri ...	1989	2682524
pulsed-field gel electrophoresis of circular dna.	mobility of supercoiled (form i) and nicked circular (form ii) plasmid dnas was determined on two major forms of pulsed-field electrophoresis, chef and ofage. plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with saccharomyces cerevisiae chromosomes as standards. agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels resolving forms i and ii of smaller plasmids. the pulsing range of 3.7 to 240 seconds resulted in quite v ...	1989	2662139
internalization and degradation of insulin by a human insulin receptor-v-ros hybrid in chinese hamster ovary cells.	chinese hamster ovary cell lines expressing either the wild-type human insulin receptor or a hybrid molecule in which the tyrosine kinase domain of the insulin receptor is replaced with that of the oncogene, v-ros were examined for their ability to internalize and degrade insulin. cells expressing the hybrid receptor were found to internalize and degrade insulin at approximately half the rate of cells expressing the native insulin receptor. moreover, insulin was incapable of inducing the interna ...	1989	2645866
short chain fatty acid-induced colitis in mice.	short-chain fatty acids (e.g. acetic, propionic and butyric acid) are metabolic products produced by colonic bacteria. since intra-rectal instillation of dilute acetic acid to rats and cats has been reported to produce a diffuse colitis similar to the human disease, in our study we have investigated the effect of intra-rectal butyric acid in mice. male bka mice (n = 6) received 01 ml of dilute butyric acid by intra-rectal instillation which was flushed out with saline after 10 sec. control anima ...	1989	2634625
in-vivo effects of (e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine (mdl 72145) on amine oxidase activities in the rat. selective inhibition of semicarbazide-sensitive amine oxidase in vascular and brown adipose tissues.	one hour after mdl 72145 ((e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine) (2.5 mg kg-1) was given by intraperitoneal injection, the semicarbazide-sensitive amine oxidase (ssao) activity of rat aorta and brown adipose tissue measured in-vitro was reduced by more than 95% of its control value, whereas the monoamine oxidase (mao-a) activity remained virtually unaffected. the action of this drug on amine oxidases in the liver at this dose was less selective. the in-vitro effect of mdl 72145 on the ...	1989	2565962
effects of toxin isolated from the sea anemone bolocera tuediae on electrical properties of isolated rat skeletal muscle and cultured myotubes.	electrophysiological effects of a polypeptide toxin isolated from the sea anemone bolocera tuediae (bttx ii) on isolated fast and slow rat skeletal muscle and on cultured rat myotubes are described in this paper. nanomolar concentrations of bttx ii cause a concentration-dependent depolarization. this effect is prevented by the presence of tetrodotoxin. action potential duration is prolonged in all preparations investigated, with the fast muscle being the least sensitive. the positive overshoot a ...	1989	2566207
differential immunohistochemical localization of cytokeratins and collagen types i and iii in experimentally-induced cirrhosis.	liver cirrhosis was induced in male wistar rats by subcutaneous injection (1 ml of 30 g/l) of an aqueous solution of thioacetamide. using the indirect immunoperoxidase technique, high molecular weight keratins were localized in bile ducts and ductules. low molecular weight cytokeratins were present in regenerating hepatocytes in active cirrhosis; bile ducts were unstained. these results suggest that cytokeratin staining may be useful in distinguishing bile duct epithelium and hepatocytes in hepa ...	1989	2478687
characterization and purification of h1tf2, a novel ccaat-binding protein that interacts with a histone h1 subtype-specific consensus element.	definition of mechanisms regulating human histone h1 gene transcription during the cell cycle requires the isolation and biochemical characterization of protein factors which interact with specific promoter elements. two distinct binding activities have been identified in nuclear extracts from hela cells and mapped within a 180-base-pair (bp) region of a cell cycle-regulated h1 gene promoter. h1tf1 bound to an h1-specific a + c-rich sequence (ac box), 100 bp upstream of the cap site; h1tf2 inter ...	1989	2725515
primary structures of sm31/32 diagnostic proteins of schistosoma mansoni and their identification as proteases.	we have constructed cdna clones containing the complete nucleotide sequences coding for two highly antigenic schistosoma mansoni adult worm proteins, sm31 and sm32. the predicted amino acid sequence of sm31 shows significant homology to mouse, rat and human cathepsin b. the nucleotide sequence of sm32 is identical to that reported by others for s. mansoni "haemoglobinase'. the different nucleotide sequences demonstrate the existence of two different proteolytic enzymes, both of which are synthes ...	1989	2725581
sugar composition of the polysaccharide portion of lipopolysaccharides of vibrio fluvialis, vibrio vulnificus, and vibrio mimicus.	a chemotaxonomic study was carried out on vibrio fluvialis and v. vulnificus on the basis of the sugar composition of the polysaccharide portion of their lipopolysaccharides (lps). a previously developed rapid method of preparing samples for compositional sugar analysis was employed. nineteen o-serogroups of v. fluvialis were divided into 14 chemotypes while seven o-serogroups of v. vulnifucus were divided also into seven chemotypes since the polysaccharide portion of lps of each serogroup has a ...	1989	2482433
ventricular arrhythmias and local electrograms after chronic regional denervation of the ischemic area in the pig heart.	cardiac denervation has been proved to reduce the incidence of coronary occlusion arrhythmias in digs, but the effect of limiting the extent of sympathectomy to the ischemic area, particularly in hearts with sparse coronary collateral circulation, as in the human heart, needs further investigation. ventricular arrhythmias and changes in epicardial direct current electrograms induced during acute left anterior descending coronary artery occlusion were recorded in 14 pigs subjected to regional den ...	1989	2738265
hemochromatosis and infection: alcohol and iron, oysters and sepsis.	hemochromatosis, or primary iron overload, is a variably expressed genetic metabolic disorder greatly modified by sex, age, diet, and alcohol consumption. although a diagnosis has been made at the bedside by careful documentation of the slow resolution of subcutaneous iron pigment, clinical diagnosis is frequently overlooked, and even autopsy may fail to reveal hemochromatosis as the cause for cirrhosis. genetic linkage studies have confirmed the extremely high prevalence of this disorder. untre ...	1989	2486533
attempted passive transfer of a serum factor in chronic urticaria from human to monkey.		1989	2757947
2-bromo- and 16-bromo-estrogens and related compounds: potential inhibitors for both estradiol 2- and 16 alpha-hydroxylases in rat liver microsomes.	kinetic studies of inhibition of estradiol 2- and 16 alpha-hydroxylase activities in male rat liver microsomes with 2-bromoestrogens, 4-bromo-estrone (4-bre1), 16 alpha- and 16 beta-bromoestrones and 16 beta-acetylthioestrone (16-acse1) were carried out. 2-bromoestrogens and 4-bre1 nonspecifically blocked the two enzyme activities in a competitive manner, and 2-bromo-estradiol (2-bre2) was the most potent inhibitor for the two hydroxylases among the 2- and 4-bromo steroids. kinetic data, the app ...	1989	2761259
transcriptional start site in the mouse cyp1a1 (cytochrome p1450) gene.	two transcriptional initiation sites have been described by different laboratories for the mouse cyp1a1 (cytochrome p1450) gene: start site ti, at the beginning of the nontranslated 87-bp first exon, and start site tii, 635 bp downstream from site ti within the 2,380-bp first intron. site ti was characterized in normal c57bl/6 mouse liver, whereas site tii was described in a high activity variant line (hav cells) derived from the mouse hepatoma hepa-1c1c7 wild type (wt) established cell line. it ...	1989	2766934
[cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control].	fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ...	1989	2772364
unproven methods of cancer management. international association of cancer victors and friends, inc.	after study of the literature and other available information, the american cancer society has found no evidence that methods of treatment promoted by the international association of cancer victors and friends, inc. (iacvf), would result in objective benefit in the treatment of cancer in human beings. lacking such evidence, the american cancer society strongly urges individuals with cancer not to seek treatment based on the recommendations of iacvf.	1989	2492878
suppression of antibody responses to the acetylcholine receptor by natural antibodies.	some relationships between "natural" physiological autoreactivity and antibody responses against the acetylcholine receptor (achr) were investigated. collections of unselected monoclonal antibodies were derived from nonmanipulated newborn mice of susceptible (balb.b) and resistant (balb/c) mouse strains, and screened for reactivity with heterologous achr. five in 200 balb/c but none in 200 balb.b clones were found reactive. four of these anti-achr antibodies were analyzed for connectivity to nin ...	1989	2776827
virulence of vibrio vulnificus strains isolated from seafoods.	a study was carried out on the relation between virulence of v. vulnificus assessed by ld50 in mice and other markers. v. vulnificus strains isolated from seafoods had ld50 ranging from 10(3)-10(7) for mice. strains showing ld50 10(6) or less were generally haemolytic and settled after boiling. resistance to bactericidal action of serum and ability to take up congo red was shown by all the strains.	1989	2777343
human eosinophils express cd4 protein and bind human immunodeficiency virus 1 gp120.	the cd4 glycoprotein, expressed on leukocytes belonging to subsets of t lymphocytes and to cells of monocyte/macrophage lineage, participates in the functioning of t cells and serves as a receptor for hiv-1 and hiv-2. human eosinophils, a class of granulocytic leukocytes, have been found to express cd4. with anti-cd4 mabs cd4 was demonstrable on eosinophils from both normal and eosinophilic donors. eosinophils synthesized a 55-kd cd4 polypeptide immunoprecipitable with two anti-cd4 mabs. eosinop ...	1989	2783333
effect of vitamin d metabolites on calcitriol metabolism in experimental renal failure.	previous studies from our laboratory have demonstrated that metabolic clearance rate (mcr) of calcitriol is decreased in experimental renal failure. in this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin d3 (25(oh)d3) and 24,25-dihydroxyvitamin d3 (24,25(oh)2d3) on the mcr of calcitriol in renal failure produced in rats by partial nephrectomy. the mcr of calcitriol in these rats with renal failure was significantly lower than in control rats with sham operations. plasma con ...	1989	2789310
activation of hageman factor and prekallikrein and generation of kinin by various microbial proteinases.	activation of the hageman factor-kallikrein-kinin system by serratial 56-kda proteinase was previously demonstrated (matsumoto, k., yamamoto, t., kamata, t., and maeda, h. (1984) j. biochem. (tokyo) 96, 739-749; kamata, r., yamamoto, t., matsumoto, k., and maeda, h. (1985) infect. immun. 48, 747-753). to investigate whether the activation of the system is specific for 56-kda proteinase or is found similarly with other microbial proteinases, 11 proteinases of microbial origins were studied; the 5 ...	1989	2499581
role of carboxylesterase in soman, sarin and tabun poisoning in rats.	tocp (triorthocresyl phosphate) inhibits carboxylesterase (carbe) activity in rat plasma and liver and significantly increases soman, sarin and tabun toxicity. application of these agents after tocp caused strong additional inhibition of carbe and cholinesterases (che) in rat red blood cells, plasma, liver, brain, diaphragm and intercostal muscle. after phenobarbital pretreatment, which induced carbe activity in plasma and liver by 80% and that of plasma che by 33%, acute toxicity of soman and t ...	1989	2813290
the dermatopathologist and human immunodeficiency virus infection.		1989	2817921
hemocyte monophenol oxidase activity in mosquitoes exposed to microfilariae of dirofilaria immitis.	monophenol oxidase (mpo) activity in hemocytes collected from aedes aegypti liverpool strain and aedes trivittatus intrathoracically inoculated with saline alone, inoculated with dirofilaria immitis microfilariae (mff), or from uninoculated mosquitoes was compared using a radiometric tyrosine hydroxylation assay. hemocyte mpo activity in mff-inoculated (= immune-activated) mosquitoes was significantly increased at 24 hr postinoculation (pi) in a. aegypti and at 6, 12, and 24 hr pi in a. trivitta ...	1989	2493084
killing of human tumor cells by antibody c3b conjugates and human complement.	to potentiate the lytic action of complement on tumor cells, we have constructed heteroconjugates composed of monoclonal antibody and of the human c3b component of complement. the conjugates were formed efficiently using the heterobifunctional cross-linking reagent spdp. the monoclonal antibody-c3b conjugate promoted the killing of k562 tumor cells by normal human serum. treatment of the tumor cells with the monoclonal antibody and normal human serum resulted in 10-15% lysis. however, following ...	1989	2519543
cell proliferation kinetics of epidermis, hair follicles, and sebaceous glands of cocker spaniels with idiopathic seborrhea.	cell proliferation kinetic values were established for the epidermis, hair follicle epithelium, and sebaceous glands of 8 cocker spaniels with primary idiopathic seborrhea. values were established by intradermal pulse labeling injections of tritiated thymidine followed by cutaneous biopsy and autoradiography. the epidermal basal cell-labeling index was 4.96 +/- 0.97%, and the epidermal nucleated cell-labeling index was 3.33 +/- 0.71%. calculated epidermal cell renewal time for the viable layers ...	1989	2533468
bile acid profile in a dog with cholangiocarcinoma.		1989	2536512
immunocytochemical study of localization and traffic of thyroid peroxidase/microsomal antigen.	we studied the distribution of binding sites for anti-peroxidase monoclonal antibody and anti-microsomal antibodies on isolated human thyroid follicles and a human thyroid cell line. both open follicles and cells were incubated first with antibodies at +4 degrees c, then with colloidal gold labelled protein a. the topography of the binding sites for monoclonal anti-peroxidase antibody corresponded closely to the expected cell surface distribution of endogenous thyroid peroxidase since labelling ...	1989	2491623
studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1).	anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ...	1989	2632089
studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1).	anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ...	1989	2632089
[serologic studies of listeriosis antibodies in dogs in greece].	in an epidemiological study of dogs carried out in greece, antibodies against listeriosis were demonstrated in the slow-agglutination and the complement-fixation-reaction. depending on the dogs' place of origin cfr titers were found in 6.1% to 51.1%. cfr-titers could be confirmed in a comparative investigation with the slow-agglutination-test.	1989	2583499
ultrastructure of the tail of echinostoma revolutum cercaria.	the ultrastructure of the tail fins and muscles of echinostoma revolutum cercaria is described. the longitudinal muscles consist of a primitive type of transversely striated muscle fibres with a well-developed sarcoplasmic reticulum in the z-band. the fins are formed by a longitudinal tegument fold containing the same components as the remaining part of the tail tegument. the difference between the body and tail tegument is discussed.	1989	2583613
pathological analyses on the compressed cord by the experimental spinal metastasis.	the purpose of the present experimental spinal metastasis developed in rats by inoculation of walker's cancer cells through the spinous process is to find the factor which causes the initial damage of the cord in this disorder. in the early stage of paralysis, microangiogram exhibited that the degenerated posterior funiculus originated from the small hemorrhagic area in the posterior column of the involved cord. scanning electron microscopic findings showed that the hemorrhage was of the intrins ...	1989	2586714
susceptibility of erythrocytes from several animal species to vibrio vulnificus hemolysin.	the hemolytic activity of vibrio vulnificus hemolysin (vvh) against erythrocytes from several animal species (sheep, horse, cow, rabbit, chicken) was investigated. vvh was active against erythrocytes from all species, but the amount of vvh causing 50% hemolysis under identical conditions (hemolytic susceptibility to vvh) differed. the degree of 125i-labeled vvh (125i-vvh) binding to each erythrocyte species correlated with the susceptibility of the cells to hemolysis. however, marked differences ...	1989	2612874
recombinant human erythropoietin treatment in patients on maintenance home haemodialysis.		1989	2570273
hydrogen peroxide mutagenicity towards salmonella typhimurium.	in bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ...	1989	2572065
characterization of rat cerebral cortical beta adrenoceptor subtypes using (-)-[125i]-iodocyanopindolol.	(-)-[125i]-iodocyanopindolol [-)icyp), used to characterize beta adrenoceptors on membrane preparations from rat cerebral cortex, was shown to have affinity for both beta adrenoceptors and serotonin receptors. therefore, 10 microm serotonin was added to the assays to prevent (-)icyp binding to serotonin receptors. under these conditions, (-)icyp binding to the cortical membrane preparation was reversible and saturable, and the association reaction was very slow. the dissociation reaction was als ...	1989	2577008
sodium and potassium currents recorded during an action potential.	a simple method was used to measure directly sodium and potassium currents underlying the action potential in single nerve fibres of xenopus laevis. a short rectangular stimulus under current-clamp conditions elicited an action potential which was digitally stored and later used as command when voltage-clamping the same fibre. the currents thus obtained nearly reproduced the original rectangular stimulus. adding first 100 nm ttx and subsequently 100 nm ttx plus 10 mm tea to the extracellular rin ...	1989	2546753
hypoxic changes in hippocampal neurons.	1. reversible effects of brief periods of anoxia (replacing 95% o2-5% co2 with 95% n2-5% co2 for 2-4 min) were studied in ca1 neurons in hippocampal slices (from sprague-dawley rats), kept in an interface-type chamber at 33.5 degree. 2. the predominant voltage change during anoxia (n2) was a hyperpolarization, accompanied by a marked fall in resistance and excitability; synaptic potentials were also depressed, especially inhibitory postsynaptic potentials (ipsps). 3. in voltage-current (v-i) plo ...	1989	2547034
enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus.	we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ...	1989	2556341
[acute maternal anterior poliomyelitis in a non-endemic zone].	the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus.	1989	2561040
the common src homology region 2 domain of cytoplasmic signaling proteins is a positive effector of v-fps tyrosine kinase function.	a conserved noncatalytic domain sh2 (for src homology region 2) is located immediately n terminal to the kinase domains of all cytoplasmic protein-tyrosine kinases. we found that the wild-type v-fps sh2 domain stimulated the enzymatic activity of the adjacent kinase domain 10-fold and functioned as a powerful positive effector of catalytic and transforming activities within the v-fps oncoprotein (p130gag-fps). partial proteolysis of p130gag-fps and supporting genetic data indicated that the v-fp ...	1989	2685548
membrane fatty acid and virulence changes in the viable but nonculturable state of vibrio vulnificus.	the nonculturable state of vibrio vulnificus and, for comparison, that of escherichia coli were studied in artificial-seawater microcosms at 5 degrees c. total cell counts were monitored by acridine orange epifluorescence, metabolic activity by direct viable counts, and culturability by plate counts on selective and nonselective media. whereas total counts remained constant, plate counts of v. vulnificus suggested nonculturability by day 24. in contrast, direct viable counts indicated significan ...	1989	2696428
vibrio vulnificus sepsis after eating raw oysters.	vibrio vulnificus, a marine vibrio found in coastal waters of the united states, may contaminate certain seafoods, particularly raw oysters. patients with underlying liver disease are particularly susceptible to severe illness. unexplained febrile diseases in patients who have eaten raw oysters may be caused by vibrio vulnificus. a fatal and a nonfatal case are reported in two such patients. these patients are the first two reported cases of vibrio vulnificus infection in kentucky.	1989	2723530
vibrio sepsis in a cirrhotic patient.	vibrio vulnificus should be suspected in any soft tissue infection occurring after exposure to the marine environment. early recognition followed by appropriate antibiotic therapy and debridement of involved tissue are essential features in the treatment of these infections.	1989	2734646
[complexing of bovine serum albumin with phosphatidyl choline liposomes].	thermodynamic parameters of the bovine serum albumin interaction with phosphatidyl choline liposomes have been determined. it is shown that the bovine serum albumin is adsorbed on the liposomal surface without change of the globule conformation, the polar interactions making dominant contribution in immobilization. the complexing is characterized by a binding constant and a great number of binding sites.	1989	2749908
[intestinal microbial ecology and its modulation under the influence of immunodepressants].	oral administration of immunodepressants such as imuran (purine analog) and batriden (gossypol derivative) for 3 months led to development of dysbacterioses in various sections of the rat gastrointestinal tract. the dysbacterioses differed in their levels and the pattern of the recovery process. as compared to batriden, imuran in a dose of 30 mg/kg body weight administered at the early observation periods (days 7, 14 and 30) induced more marked disorders in the intestine microecology. the imuran ...	1989	2802878
phagocytosis by guinea pig polymorphonuclear leukocytes of liposomes stabilized with polysaccharides.	hemolysate-loaded liposomes were prepared with their surface coated by polysaccharides, carboxymethylchitin, carboxymethylcellulose and carboxymethylchitosan. phagocytosis by guinea pig polymorphonuclear leukocytes of these coated liposomes was measured in view of electrostatic interactions between the cells and the liposomes. this view revealed to hold at least in quite an early stage of phagocytosis, but some specific interactions were assumed to exist between the cells and the liposomes. n-ac ...	1989	2775873
human milk feeding enhances the urinary excretion of immunologic factors in low birth weight infants.	the effects of fortified human milk feedings on the urinary excretion of lactoferrin, lysozyme, secretory component, iga, and secretory iga antibodies to escherichia coli o antigens were investigated in very low birth wt infants. infants were maintained on either a human milk or a cow's milk preparation. the amounts of each immune factor that were ingested and excreted were quantified during balance studies conducted at 2.5 and 5 wk of age. serum levels of these immune factors were similar in bo ...	1989	2919134
vibrio vulnificus infections.		1989	18750602
basic training and the part i examination.		1989	267474
specificity and sensitivity of technetium-99m pyrophosphate myocardial imaging in acute myocardial infarction.		1989	203178
quality touching to communicate caring.		1988	259270
new selective and differential medium for vibrio cholerae and vibrio vulnificus.	[this corrects the article on p. 2263 in vol. 53.].	1988	16347534
what schedule-induced polydipsia can tell us about alcoholism.	an animal model of chronic and excessive voluntary (unforced) alcohol ingestion is presented in which, by drinking, animals produce repeated, substantial elevations in blood ethanol concentration and develop physical dependence. the overindulgence is elective in that ethanol is chosen in preference to certain other fluid-ingestive alternatives. beside the usual demonstrations of acutely compromised motor performance, tolerance development, cross-tolerance, etc., the model demonstrates that the c ...	1988	3067598
what schedule-induced polydipsia can tell us about alcoholism.	an animal model of chronic and excessive voluntary (unforced) alcohol ingestion is presented in which, by drinking, animals produce repeated, substantial elevations in blood ethanol concentration and develop physical dependence. the overindulgence is elective in that ethanol is chosen in preference to certain other fluid-ingestive alternatives. beside the usual demonstrations of acutely compromised motor performance, tolerance development, cross-tolerance, etc., the model demonstrates that the c ...	1988	3067598
in vivo electrochemical detection of 5-hydroxyindole within the trigeminal nucleus caudalis of freely moving rats: the effect of morphine.	the trigeminal nucleus caudalis is considered the equivalent of the orofacial nociceptive system of the dorsal horn of the spinal cord. at the level of this trigeminal area (i.e. medullary dorsal horn) the transmission of noxious inputs is strongly modulated by a descending, serotonergic system mainly originating from the nucleus raphe magnus (nrm). the present study in freely moving animals reports the effect of morphine on the 5-hydroxyindole oxidation current recorded in the medullary dorsal ...	1988	3196919
probable detection of kil peptide derived from colicin e1 plasmid in the envelope fraction of escherichia coli hb101 carrying peap31.	escherichia coli carrying plasmid peap31 produces extracellularly alkalophilic bacillus penicillinase encoded on the plasmid. the extracellular production has been suggested to be caused by activation of dormant colicin e1 kil gene. two peptides that could be respectively precursor and mature products of colicin e1 kil gene were detected on an sds/polyacrylamide gel. one of the peptides (mr 4800), which was probably a precursor peptide, was detected in the inner-membrane fraction from the organi ...	1988	3058121
probable detection of kil peptide derived from colicin e1 plasmid in the envelope fraction of escherichia coli hb101 carrying peap31.	escherichia coli carrying plasmid peap31 produces extracellularly alkalophilic bacillus penicillinase encoded on the plasmid. the extracellular production has been suggested to be caused by activation of dormant colicin e1 kil gene. two peptides that could be respectively precursor and mature products of colicin e1 kil gene were detected on an sds/polyacrylamide gel. one of the peptides (mr 4800), which was probably a precursor peptide, was detected in the inner-membrane fraction from the organi ...	1988	3058121
high-molecular-weight neutral proteinase of rat ascites hepatoma cells and their collagenolytic activity.	a high-molecular-weight proteinase in rat ascites hepatoma ah109a cells was purified to apparent homogeneity by conventional chromatographic techniques. the purified proteinase degraded over the broad ph range, 7.0-9.0, not only denatured hemoglobin but also type i and iv collagen in the absence of calcium. its activity was maximum at ph 8.0, heat-labile and affected by thiol reagents. serine proteinase inhibitors such as diisopropyl fluorophosphate, soybean trypsin inhibitor, leupeptin, and ant ...	1988	3058624
vitamin e deficiency and neurologic disease.	during the recent resurgence of interest in the clinical uses of vitamin e, one of the major foci of attention has been the neurologic role of vitamin e in humans. studies in patients with secondary vitamin e deficiency, caused by fat malabsorption disorders and total parenteral nutrition lacking an adequate supply of vitamin e, have elucidated a clinical disorder and histologic lesions of the nervous system and muscle that closely resemble those of experimental vitamin-e-deficient animal models ...	1988	3060170
[clinical studies of rokitamycin dry syrup on skin and soft tissue infections in the pediatric fields].	rokitamycin (rkm) dry syrup, a newly developed macrolide antibiotic, was administered to children with ages between 6 months and 15 years and 10 months suffering from skin and soft tissue infections including 41 cases of impetigo, one case of staphylococcal scalded skin syndrome (ssss) and 2 cases of subcutaneous abscess totalling 44 cases. the average daily dose level used was 31.3 mg/kg divided into 3 or 4 doses, for an average of 6 days of treatment. mics of 4 different macrolide antibiotics ...	1988	3204657
vibrio vulnificus and pulmonary infection.		1988	3195881
the effect of alcoholic beverages on iron and zinc metabolism in the rat.	1. male wistar rats (approximately 200 g) were given distilled water and a semi-synthetic control diet for 6 d. on day 7, 37 kbq 65zn were administered intramuscularly and the rats were given distilled water, beer, cider, red wine, whisky or ethanol as their only source of fluid. the wine, whisky and ethanol were diluted so that each of the beverages contained a similar ethanol concentration (approximately 30 g/l). food and fluid intake, growth rate and whole-body 65zn were measured regularly ov ...	1988	3196669
acute hepatic failure precipitated in a patient with subclinical liver disease by vibrionic and clostridial septicemia.	a case of fatal septicemia caused by vibrio vulnificus and clostridium bifermentans in a patient with subclinical liver disease is described. the patient appeared to recover from the infection initially after antibiotic therapy but finally succumbed to rapidly developing hepatic failure. postmortem examination revealed hemochromatosis. the pathogenesis of the polymicrobial septicemia and hepatic failure is discussed in the light of the published literature.	1988	3211597
a case of vibrio vulnificus infection.	vibrio vulnificus, a recently described strain of the halophilic vibrio species, was isolated from the blood of a 73-year-old man, who developed rapidly progressive wound infection and fatal septicemia. twenty-one patients with vibrio vulnificus infection have been reported in the japanese literature. vibrio vulnificus most frequently causes primary septicemia and necrotising cellulitis after the eating of raw fish or shellfish or after exposure to seawater. the infection is characterized by its ...	1988	3068394
role of the protease in the permeability enhancement by vibrio vulnificus.	the protease produced by vibrio vulnificus enhances vascular permeability through histamine release from mast cells and activation of the plasma kallikrein-kinin system which generates bradykinin when injected into the dorsal skin. v. vulnificus living cells also enhanced vascular permeability within a few hours after the injection into the dorsal skin. the permeability-enhancing activity of living cells was greatly reduced by addition of soybean trypsin inhibitor, a specific inhibitor for plasm ...	1988	3068493
antibody synthesis specific for nonoral antigens in inflamed gingiva.	in vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal b-cell activators (pba). we reasoned that if pba were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate. to test this, rabbits with experimental periodontitis were immunized in the hind legs with the histochemically detectable antigen horseradish peroxidase (hrp) or glucose oxidase ( ...	1988	3257940
protective immunity evoked by oral administration of attenuated aroa salmonella typhimurium expressing cloned streptococcal m protein.	attenuated strains of salmonella have been used effectively as vaccines against typhoid fever. we have investigated the use of such strains to deliver cloned antiphagocytic virulence determinants of unrelated bacteria. the aroa strain of s. typhimurium sl3261 was transformed with a low-copy plasmid vector pmk207, which contains the cloned gene spm5 encoding streptococcal m protein, the major virulence factor of these organisms. the transformed sl3261 expressed type 5 m protein in the cytoplasmic ...	1988	3294331
ability of vibrio vulnificus to obtain iron from hemoglobin-haptoglobin complexes.	it has been suggested that the normal serum protein, haptoglobin (hp), serves a bacteriostatic role by binding free hemoglobin (hm), thus making heme iron unavailable for bacterial growth. previous studies showed that, unlike escherichia coli, vibrio vulnificus was able to overcome this hp-blocking effect. we report here a study on the iron-withholding property of the three major human hp phenotypes, hp 1, 2, and 2-1. results of experiments with human serum showed that v. vulnificus c7184 was ab ...	1988	3335405
alterations in aortic endothelial cell morphology and cytoskeletal protein synthesis during cyclic tensional deformation.	previous studies have shown that bovine aortic endothelial cells (ecs) in culture respond to repetitive tensional deformation with an increase in deoxyribonucleic acid synthesis and cell proliferation. this study was designed to determine whether cyclic tensional deformation of ecs in vitro induces different morphologic or protein synthetic responses. ecs from passages 6 through 9 were seeded in 35 mm2 well silicone rubber plates at 2 x 10(5) cells/well and allowed to attach for 24 hours. the ex ...	1988	3336119
light- and electron-microscopic analysis of the kidney in newborn rats exposed to gentamicin in utero.	kidneys of newborn rats, whose mothers were given 75 mg/kg of gentamicin daily from the tenth day of gestation to its term, were examined by light and electron microscopy, as were kidneys of control rats whose mothers were given saline during the same period. in the gentamicin-treated group, the numbers of differentiated glomeruli and of s-shaped bodies counted in a median cross-section of the kidney were reduced, compared with those in the control group, but the glomerular volume was the same. ...	1988	3337212
iron metabolism in the erythrophagocytosing kupffer cell.	like the peritoneal macrophage, the isolated kupffer cell is capable of processing and releasing iron acquired by phagocytosis of immunosensitized homologous red blood cells. when erythrophagocytosis is restrained to levels which do not affect cell viability, or less than 1.5 red cells/macrophage (phagocytic index of 150%), over 40% of iron acquired from red cells is released within 24 hr. more active erythrophagocytosis results in greater release of iron but progressive deterioration in cell vi ...	1988	3338718
expression of heme oxygenase gene in rat and human liver.	developmental changes of microsomal heme oxygenase were studied. in human fetal liver, the enzyme activity was 8 times higher than that detected in the adult liver. on the other hand, adult livers contained 4 times more cytochrome p450 than fetal livers. elevated heme oxygenase activity in fetal liver was not due to modulators present in the microsomes. similar to human, rat fetal liver also contained high enzyme activity which appeared to be regulated at the transcriptional level. hybridization ...	1988	3342043
hydrogen transfer by nadph-dependent reductases in elongation of very-long-chain saturated and polyunsaturated fatty-acyl-coa in swine cerebral microsomes.	the hydrogen transfer from nadph was studied in the elongation of arachidoyl-coa (20:0-coa) and arachidonoyl-coa (20:4-coa) in swine cerebral microsomes. previously, we showed that four deuterium atoms (2h) were transferred stereospecifically from (4s)-[4-2h1]nadph in the elongation of 20:0-coa to 24:0 (yoshida, s., takeshita, m. and kawaguchi, a. (1984) biochem. biophys. res. commun. 124, 322-328), and that three deuterium atoms were transferred from 2h2o in the elongation of 20:4-coa to 22:4. ...	1988	3342246
dna binding by 1-nitropyrene and 1,6-dinitropyrene in vitro and in vivo: effects of nitroreductase induction.	1-nitropyrene, the predominant nitropolycyclic aromatic hydrocarbon found in diesel exhaust, is a mutagen and tumorigen. 1,6-dinitropyrene is present in diesel exhaust in much smaller quantities than 1-nitropyrene, but is much more mutagenic and carcinogenic. in an attempt to understand this difference in biological potencies, we have compared the extent of dna binding by these two nitropyrenes in vivo. we have also determined the effect of 1-nitropyrene pretreatment upon the induction of nitror ...	1988	3345577
electron microscope radioautographic evidence of in vivo androgen-binding protein internalization in the rat epididymis principal cells.	the androgen-binding protein (abp) has been purified from rat testes with a yield of 14% using four steps of hplc and was subsequently iodinated to a specific activity of 0.1 mci/mg protein. using a micromanipulator, [125i] iodo-abp-dihydrotestosterone was injected intraluminally into the proximal caput of the rat epididymis. epididymides were sampled from 3 to 120 min after the injection of the tracer and processed for transmission electron microscopy autoradiography. our results showed the acc ...	1988	3345715