Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [triiodothyronine thyrotoxicosis in nodular toxic goiter]. | serum protein-bound iodine (pbi), total blood thyrotoxin (t4), triiodthyronine (t3), coefficient of thyrotoxin efficacy (cte), serum thyrotoxin-binding capacity (tbc) or some of these indices were determined in 44 patients with nodular toxic goiter with thyrotoxemia. an increase in t3 level was revealed in 27 of 35 of the patients; t3-thyrotoxicosis was diagnosed in 18 of these in whom an increase of the t3 level was combined with normal t4 or normal cte content and an unchanged tbc. the symptom ... | 1976 | 59358 |
| placento-thyroidal relationship in normal pregnancy. | estimations of serum hct, htsh, t4, t3, pbi, etr, triosorb, tbg-binding capacity, bmr and urinary total estrogen were made simultaneously in 160 women in normal pregnancy. trh stimulation tests were made in 20 cases in each trimester of pregnancy. hct was detectable even in early pregnancy, tending to increase gradually toward the terminal stage of pregnancy as serum thyrotrophin bioactivity showed. on the other hand, serum tsh level measured by radio-immunoassay remained essentially the same th ... | 1976 | 59514 |
| pituitary-thyroid regulation in euthyroid patients with graves' disease previously treated with antithyroid drugs. | to understand why some patients with hyperthyroidism due to graves' disease remain euthyroid after a course of antithyroid drug therapy, pituitary-thyroid regulation was studied in 20 such patients who had remained well for six months or longer after the withdrawal of antithyroid drugs. only patients who were clinically euthyroid and had normal serum thyroxine (t4), triiodothyronine (t3), and thyrotropin (tsh) concentrations were studied. serum tsh responses to thyrotropin-releasing hormone (trh ... | 1976 | 59733 |
| humoral immune responses in rana catesbiana frogs and tadpoles. | rana catesbiana adult frogs and tadpoles were immunized with the bacteriophage f2, 0x-174, and t4 and the haptens 2,4 dinitrophenyl (dnp) and fluorescein (ftc). the haptens were conjugated with bovine serum albumin (bsa), bovine gamma globulin (bgg), or horsehoe crab hemocyanin (hycn). sera were obtained from immunized animals at invervals up to six months after immunization. the antibody activities were measured by bacteriophage neutralization techniques. sucrose density gradients were used to ... | 1976 | 59790 |
| familial partial target organ resistance to thyroid hormones. | a 30-year old woman with a history of recurrent goiter, who had undergone two partial thyroidectomies, is described. she presented with tachycardia, nervousness and a fine tremor of the fingers. initially, she had normal serum thyroid hormone levels: thyroxine (t4 (d)) 11.6 mug/100 ml, triiodothyronine (t3) 138 ng/100ml, normal levels of binding proteins and a very high serum thyrotropin (tsh), 98 muu/ml. during follow-up t4 (d) increased to 17.2 mug/100 ml, t3 increased to 277 ng/100 ml, while ... | 1976 | 60346 |
| inhibition of specific binding of dnp (dinitrophenyl) determinant to lymphoid-cell receptors by related and unrelated compounds : quantitative studies in vitro. | the effect of related and unrelated compounds on the specific binding of dinitrophenyl-coupled bacteriophage (dnp-t4) to lymphoid cell receptors has been examined and compared with the effect on the neutralization of dnp-t4 by anti-dnp serum. spleen cells and sera from balb/c mice immunized with dnp-bovine serum albumin were used. the binding of dnp-t4 to the cells was inhibited by dnp-eacp, di-dnp-lys, dnp-tyr, dnp-p(ornith) and dnp-bsa (among the dnp-derivatives tested), tnp-bsa, ars-p(tyr) an ... | 1976 | 60907 |
| temporary hypothyroidism after surgical treatment of thyrotoxicosis. | mild clinical hypothyroidism associated with low levels of serum total thyroxine (t4) and tri-iodothyronine (t3) and raised levels of serum thyroid-stimulating hormone (t.s.h.) has been observed in 14 of 40 patients (35%) in the early months after a subtotal thyroidectomy for thyrotoxicosis under cover of propranolol. in 10 of the patients, however, the hypothyroidism was temporary and at 6 months after operation the thyroid hormone levels were normal and the serum t.s.h. levels had fallen. in 4 ... | 1976 | 61495 |
| hct and thyroid function in molar pregnancy. | hct, tsh, t3, t4, pbi, t3-rsu, tbg binding capacity, bmr and thyroid uptake of radioiodine were measured simultaneously on the volunteers with hydatidiform mole and normal pregnancy. trh stimulation tests were performed on the two groups. the serum hct levels were higher in association with molar pregnancy than in normal pregnancy. tsh levels were within the nonpregnant range in the two groups. t3, t4, pbi, etr, bmr and thyroid uptake of radioiodine suggested thyroid hyperfunction in molar pregn ... | 1976 | 61702 |
| preclinical hyperthyroidism in multinodular goiter. | the thyrotropin (tsh) response to thyrotropin-releasing hormone(trh) (200 mug iv) was determined in 80 surgical patients with nontoxic multinodular goiter. the tsh reserve was normal in multinodular goiter. the tsh reserve was normal in 55 and elavated in 8 patients. no tsh response to trh (deltatsh less than or equal to 1 muu/ml) was detectable in 17 patients (21%). individual and mean serum t4, ft4i and serum t3 values did not differ from normal in 13 of the trh unresponsive patients; in 4 pat ... | 1976 | 61970 |
| [age dependent morphokinetics of the thyroid gland. histochemical, morphometric, light-microscopical and functional investigations (author's transl)]. | the aim of the animal experiments was to investigate the question of the age dependence of the morphokinetic reactions of the thyroid gland under standardised conditions. through the administration of pharmaceutical substances, it was possible to produce defined functional states of the thyroid in the various age groups. materials and methods 700 albino rats of the chbb: thom (spf) strain, age 0 days (newborn) to 110 weeks, were used in the investigations. the animals were divided into 4 groups ... | 1976 | 62467 |
| [malignant disease of the paranasal sinuses (author's transl)]. | during the period 1931 to 1974 167 men and 97 women with tumours of the maxilla or of the paranasal sinuses were treated at the department of radiotherapy and oncology, lainz hospital. the peak incidence is found in the age group 51 to 70 years. the survival rate is determined by the extent of spread of the tumour. cases in which the malignant process is still localized in the mucosa, without infiltration of the adjacent bony structures, have a good prognosis and so have the early cases of all t ... | 1976 | 63183 |
| effects of small doses of l-thyroxine and triiodo-l-thyronine on pituitary lh content of thyroidectomized rats. | pituitary lh was studied by means of a specific radioimmunoassay (ria) in male rats at different time intervals after thyroidectomy (t), and in rats which were t at least 30 days before and were then treated with different doses of l-thyroxine or triiodo-l-thyronine. a decrease in the pituitary lh of the t animals, with respect to the intact age-paired controls, was demonstrable from 13 days after the operation, when total pituitary lh content was taken into consideration, or from 5 days after t ... | 1976 | 63208 |
| rapid decrease in plasma-triiodothyronine during surgery and epidural analgesia independent of afferent neurogenic stimuli and of cortisol. | changes in circulating triiodothyronine (t3), thyroxine (t4), binding of thyroid hormones to plasma proteins (resin-t3 test), cortisol, and glucose were evaluated in sixteen patients undergoing abdominal hysterectomy. in eight of the patients afferent neurogenic impulses from the surgical area were blocked during and after operation by epidural analgesia. these patients were pain-free, and the normal stress-induced increase in cortisol and glucose was abolished. during epidural analgesia and gen ... | 1976 | 63806 |
| single-drug chemotherapy of bladder cancer with adriamycin, vm-26 or bleomycin. a phase ii multicentric, co-operative study. | single-drug chemotherapy was employed in a multicentric co-operative trial. adriamycin was given to 18 patients, vm-26 to 30 and bleomycin to 33. almost all patients were suffering from a t3 or t4 bladder cancer with known metastases in almost 40% of cases. the vast majority of patients had already received previous treatment either chemotherapy, surgery of irradiation. complete regression was obtained in 5% of cases; partial objective regression in 11.1% of patients treated with adriamycin, 26. ... | 1976 | 70352 |
| [evaluation of the method of blood tyroxine determination with the aid of non-immunosorbents]. | tetralute 125i-column is a set for determination of the total thyroxin content in the serum; this is a valuable test for examination of the t4 content in the blood with the aid of radioactive isotopes. the method is simple, specific, sensitive and precise, and can be used both under laboratory and under clinical conditions. results of this test coordinate with the data of the protein-bound iodine study and the percentage of the t3 absorption. the work is based on determination of t4 in 2050 samp ... | 1976 | 66676 |
| total and free triiodothyronine and thyroid-binding globulin concentration in elderly human persons. | total thyroxine (tt4) and triiodothyronine (tt3) were found to be low in healthy elderly subjects with a preferential decrease of triiodothyronine. in order to determine the importance of these findings 22 healthy elderly subjects were examined. free triiodothyronine (ft3), thyroid binding globulin (tbg) concentration and basal thyroid stimulating hormone (tsh) were measured by radioimmunoassay. liver enzymes, cholesterol and total protein concentration were also assayed. tbg was significantly i ... | 1976 | 57057 |
| radioimmunoassay for serum thyroxine-binding globulin: results in normal subjects and in patients with hepatocellular carcinoma. | serum thyroxine-binding globulin (tbg) was measured by radioimmunoassay. the human tbg used in this study was purified by affinity, anion-exchange, and gel filtration chromatography. the serum tbg concentration in 98 euthyroid normals was 1.48 +/- 0.46 mg/100 ml (mean +/- sd), which is one-half that previously reported using a similar method. the level in females (1.66 +/- 0.56) was significantly higher than that in males (1.37 +/- 0.37). comparison of the serum tbg level and the maximum binding ... | 1976 | 57964 |
| immunoassay of human tsh using dried blood samples. | a sensitive, semi-quantitative radioimmunoassay method to screen for elevations of tsh concentration in blood is described. the method requires two 0.32 cm dots of dried blood-impregnated filter paper (equivalent to 3 mul plasma) and a 3-day incubation. separation of bound and free is obtained using polyethylene glycol. the method can recognize tsh concentrations as low as 22 muu/ml using a highly sensitive antiserum developed by one of us (afp). tsh in dried cord and newborn blood from 4 infant ... | 1976 | 57965 |
| evaluation of three thyroid-function screening tests for detecting neonatal hypothyroidism. | three thyroid-function detection methods were evaluated concomitantly for possible application as routine screening tests for the early diagnosis of neonatal hypothyroidism. dried capillary-blood thyroxine (t4) was measured on eluted filter-paper discs for 9734 3-5-day old neonates. serum t4 and thyroid-stimulating hormone (t.s.h.) assays from cord blood were studied as alternative screening tests on 4911 and 3733 infants, respectively. to avoid false-negative results, neonatal blood-t4 and cord ... | 1976 | 58257 |
| reduced active thyroid hormone levels in acute illness. | in acute and subacute disease the active thyroid hormones, triiodothyronine (t3) and tetraiodothyroacetic acid (tetrac), are decreased while serum-thyroxine (t4) levels tend to be slightly reduced. conversely, the inactive metabolite, reverse triiodothyronine (reverse t3), is increased indicating a diversion of t4 metabolism from an activating to an inactivating pathway. with convalescence the serum levels of t3, tetrac, and t4 recover while reverse t3 decreases to normal. these changes occur wi ... | 1976 | 73636 |
| an assessment of daily production and significance of thyroidal secretion of 3, 3', 5'-triiodothyronine (reverse t3) in man. | while 3, 3', 5'-triiodothyronine (reverse t3, rt3) has been detected both in human serum and in thyroglobulin, no quantitative assessment of its metabolic clearance rate (mcr), production rate (pr), or secretion by the thyroid is yet available. this study examines this information in euthyroid subjects and evaluates it in light of similar information about two other iodothyronines in the thyroid: 3, 5, 3'-triiodothyronine (t3) and thyroxine (t4). thus, it was noted that rt3 is cleared from human ... | 1976 | 932209 |
| [citrate values in the whole blood of patients with malignant tumors of various locations]. | based on reports on elevated concentrations of 2-oxoglutarate in the blood of human subjects with tumors of various locations, data are presented on the concentration of citrate and pyruvate in the whole blood of 41 patients with malignant tumors (mainly of the female breast and of the digestive tract), 30 patients with benign surgical diseases and 12 healthy subjects. adopting a level of significance of 2p=0.10 differences of concentration were found between the following collectives: (1) the m ... | 1976 | 933983 |
| [value of carcino-embryonic antigen assay in the evaluation and surveillance of breast cancer]. | with the method used, the concentrations of carcinoembryonic in antigen serum can be said to be abnormal above 10 ng/ml. the concentrations are normal in benign mastopathies. in 131 cases of breast cancer were studied, they are high in 8 p. cent of t1 and t2 breast cancers, and in 53.9 p. cent of t3 and t4 cancers. they are high in 22 p. cent of cases in the first perceptible phase of their disease, and 92 p. cent relapsed cases. they are also high in 23 p. cent of cases without metastasis, in 3 ... | 1976 | 934839 |
| thyroxine and triiodothyronine in blood after ingestion of iodinated casein by chicks. | white leghorn cockerels were fed a broiler ration containing 0 (controls), 0.02 or 0.04% protamone (iodinated casein) either continuously or for 10-14 days after which the 0.02% protamone ration was switched to control or vice versa. body growth rate was temporarily suppressed during the first 14 days of feeding 0.02% protamone. thyroid weight was promptly depressed during feeding of protamone and increased more slowly when protamone was removed from the feed. only a fleeting increase in oxygen ... | 1976 | 935013 |
| [irradiation of the thyroid gland: changes in its function]. | the authors have studied some usual parameters of the evaluation of the thyroid gland function, after secondary irradiation during tele-cobaltherapy treatment in 20 patients with larynx carcinoma. the parameters studied have been iodine uptake, hamolsky index, t4, ft4 pb127i and cholesterol. the values, statistically treated, enable the conclusion that normal adult thyroid gland has a very low clinical and analytical radiosensibility. | 1976 | 935726 |
| statistical manipulation for normalization of data: in vitro thyroid function tests. | interest in chemical and statistical methods chosen to define "normal" populations for the clinical significance of tests has grown recently. because few clinical values are distributed in gaussian fashion, recommendations for smoothing of data by transforms have been prepared. in this paper we examine mathematical transformations as they are applied to in vitro tests of thyroid function and evaluate the use of multivariate regression analysis. mathematical transforms used included square root, ... | 1976 | 935913 |
| genetic analysis of t4 tail fiber assembly. i. a gene 37 mutation that allows bypass of gene 38 function. | 1976 | 936481 | |
| effect of ts mutation in gene 43 of bacteriophage t4 on recombination of the bacteriophage. | certain ts mutations in gene 43 of bacteriophage t4 are known to exhibit mutator or antimutator activities with respect to different rii mutations of this phage. the effect of these mutations on recombination frequencies of double mutant strains of phage t4 with genotype rii ts--homoallelic with respect to the ts trait--was examined. the results implicate the essential role of the genetic background in the investigated process. | 1976 | 937086 |
| purification and properties of bacteriophage t4-induced rna ligase. | 1976 | 938049 | |
| thyroid function and insulin secretion from the perfused pancreas in the rat. | the influence of thyroid function on the kinetics of glucose-induced insulin secretion from the isolated perfused rat pancreas has been studied. l-thyroxine (l-t4) administration did not modify the immediate insulin secretory response of the perfused pancreas to glucose. l-triiodothyronine (l-t3) treatment as well as propylthiouracil (ptu) treatment decreased the immediate insulin secretory response of the pancreas slightly. only thyroidectomy (tx) reduced the immediate secretory response of the ... | 1976 | 939192 |
| variations in the response of the thyroid gland of the rat to different low-iodine diets: correlation with iodine content of diet. | large variations are frequently encountered in the thyroidal responses of rats to commercially available low-iodine diets. the major aim of this investigation was to attempt to correlate these variations with differences in the iodine content of the diets. a method was developed for measuring the iodine content of low-iodine diets which was sufficiently sensitive to discriminate between a diet containing 15-20 ng of iodine per g and one containing 30-40 ng of iodine per g. large differences were ... | 1976 | 939196 |
| formation of 3,3'-diiodothyronine and 3',5',3-triiodothyronine (reverse t3) in thyroid glands of rats and in enzymatically iodinated thyroglobulin. | it was observed in the present investigation that labeled thyroxine (t4) comprised less than 2% of the total 131i in the thyroids of severely iodine-deficient rats labeled with 131i for 18-24 h, a much lower value than had previously been reported for iodine-deficient rats. this low value was attributable to two factors: 1) the use of a diet low enough in iodine content to produce extreme iodine deficiency, and 2) the use of a paper chromatography system that successfully separates t4 from the m ... | 1976 | 939197 |
| decreased pituitary growth hormone content in rats treated neonatally with high doses of l-thyroxine. | growth hormone and thyrotrophic hormone content have been measured by specific radioimmunoassays in anterior pituitaries of 22 day old rats. these animals were injected with saline or very high doses of l-thyroxine during the neonatal period in order to induce the "neo-t4" syndrome. growth of such animals is known to be affected. it was found that not only tsh but also gh content of the t4-treated animals was significantly lower than that of the saline-injected controls. | 1976 | 939463 |
| serum thyrotropin, prolactin, and growth hormone levels during the early neonatal period in the human infant. | normal term newborn infants have been studied during the first four hours after birth. cord blood and brachial venous blood samples were collected for measurement of serum prolactin (n = 15; 11 vaginal delivery; four cesarean section delivery), thyrotropin (n=8), growth hormone (n=7), tri-iodothyronine (n=7) and thyroxine (n=7). the mean cord serum tsh concentration was 12 +/- 2 muu/ml and peaked in all infants 30 minutes after birth (67 +/- 7 muu/ml). blood prl also increased in all of the 15 i ... | 1976 | 940027 |
| a mutation that confers temperature sensitivity on the translation of riib in bacteriophage t4. | 1976 | 940158 | |
| separation of labelled iodide, iodoprotein, iodotyrosines, and iodothronines in serum from patients treated with iodine-131, using an improved chromatographic method. | a method is described for complete separation of iodoprotein, 3-iodotyrosine (mit), 3,5-diiodotyrosine (dit), 3,5,3-triiodo-l-thyronine (t3), and l-throxine (t4) on a single column run on sephadex g-25 superfine in alkaline solution. sera from patients treated with 131i- have been analysed by this method after removal of i- by resin dialysis. | 1976 | 940981 |
| site of cleavage of superhelical phix174 replicative form dna by the single strand-specific neurospora crassa endonuclease. | experiments with the neurospora crassa single strand-specific endonuclease have provided evidence for the existence of regions of partially single-stranded character in covalently closed superhelical replicative form dna of phix174. the nuclease converts the superhelical molecules to either singly hit relaxed circular or doubly hit linear molecules. we show that the initial cleavage of phix174 superhelical dna is a "nick" bounded by a 5'-phosphate and a 3'-hydroxyl; no nucleotides are excised as ... | 1976 | 942717 |
| triiodothyronine radioimmunoassay and its application to thyroid disorders. | an accurate, highly sensitive triiodothyrone (t3) radioimmunoassay system is described. direct measurement in serum is made possible by the use of t3-free serum for standards, and blocking of t3 binding to thyroxine (t4)-binding globulin with salicylate. t4 cross reactivity was less than 0.3%. mean t3 levels in 55 euthyroid, 32 hyperthyroid and 19 primary hypothyroid patients were 1.46 +/- 0.17, 5.34 +/- 1.86 and 0.49 +/- 0.34 (sd) ng/ml, respectively. t3 was found to be a more sensitive index t ... | 1976 | 943381 |
| biochemistry of dna-defective mutants of bacteriophage t4. vi. biological functions of gene 42. | bacteriophage t4 gene 1 and 42 amber mutants (defective in deoxynucleoside monophosphate kinase and deoxycytidylate hydroxymethylase, respectively) are able to synthesize dna in cell-free lysates prepared as described by barry and alberts (1972), in contrast to their inabliity to do so in plasmolyzed and toluenized cell systems. addition of extracts containing an active gene 1 or 42 product has no effect on synthesis in lysates defective in the respective gene. thus, if these enzymes do play add ... | 1976 | 943575 |
| transcriptional control of t4 coliphage-specific genes 30, 42, 43, riia, riib, and e. | escherichia coli b/r (suo) was infected, at 30 degrees c, with t4dam+, t4damb24-amn82 (i-, 44-, dna-negative phenotype), and t4damn134ambl292 (33-, 55-, maturation-defective phenotype). a genetic ('transformation') assay was used to monitor transcription of genes 30 (polynucleotide ligase), 42 (deoxycytidylate hydroxymethylase), 43 (dna polymerase), riia, riib, and e (endolysin). the principal results are: (i) all of the genes studied were transcribed exlusively from the so-called l-strand of ph ... | 1976 | 945327 |
| ehanced biliary thyroxine excretion in rats treated with pregnenolone-16alpha-carbonitrile. | normal rats were treated with pregnenolone- 16alpha - carbonitrile (pcn) 10 mg/100 g by stomach tube twice daily for 3 days. in these animals the biliary excretion of intravenously injected 125i-thyroxine (t4) was enhanced and the bile: plasma 125i ratio (b/p ratio) and the biliary clearance rate of plasma 125i-t4 was increased. normal rats were treated with pcn for 3 days and homozygous gunn rats for 13 days. in both groups pcn enhanced the bile flow and elevated the b/p ratios and the biliary ... | 1976 | 946136 |
| iodothyronine content of human thyroid albumin. | stable thyroid hormones (t4 and t3)1) have been demonstrated in pure albumin isolated from normal human thyroid tissue iodinated in vivo. five samples of albumin were separated from other thyroid proteins by acrylamide gel electrophoresis. after pronase hydrolysis, the content of thyroid hormones was measured chemically (t4 + t3) as well as by competitive radioactive measurement (t4) and radioimmunoassay (t3). the purity of the albumin and validity of these measurements were confirmed by differe ... | 1976 | 946147 |
| studies on the mechanism of action of potassium iodide on thyroid protein biosynthesis. | potassium iodide (ki) has been shown to have an antigoitrogenic action and to inhibit in vivo thyroid protein biosynthesis. beef thyroid slices were used to clarify further the mechanism of action of ki. incubations were performed in krebs-ringer-bicarbonate (krb) buffer under 95%o2 and 5% co2. ki caused a slight decrease in the uptake of [3h]eucine by the tissue. when labelled leucine incorporation into protein was measured it was found that 10(-6) m ki caused a marked inhibition. increasing co ... | 1976 | 946922 |
| hyperthyroidism in graves disease. current trends in management and diagnosis. | the radioimmunoassay for t3 is now widely available and is a useful diagnostic tool for hyperthyroidism, especially in t3-thyrotoxicosis. it is an essential tool in the management of hyperthyroidism that persists after treatment with normal t4 serum levels or, in euthyroid cases, with low t4 serum levels. in these conditions, it reflects the metabolic state more accurately than serum levels of t4. a promising new test is the response of radioimmunoassayable tsh to protirelin (trh) administration ... | 1976 | 946941 |
| the effects of ergocornine on tail height, spontaneous and t4-induced metamorphosis and thyroidal uptake of radioiodide in neotenic ambystoma tigrinum. | 1976 | 946962 | |
| reciprocal effects of thyroxine and triiodothyronine on their deiodination by rat tissues in vitro. | the reciprocal effects of loading doses of thyroxine (t4) or triiodothyronine (t3) on the deiodination of their 125iodine-labelled isotopes by rat muscle and liver homogenates were studied. in 21 experiments muscle homogenates deiodinated a mean 45.0% of a tracer dose of [125i]t4 and 18.0% of [125i]t3. on addition of graded amounts of nonradioactive t4 or t3 the percentual deiodination of both labelled hormones progressively declined. this effect was significantly greater in homogenates incubate ... | 1976 | 947124 |
| the deiodination of thyroxine in hyperthyroid rats as determined by renal clearance of iodide. | other investigators have reported that whole body clearance of thyroxine (t4) is increased in hyperthyroid rats isotopically equilibrated with radioactive t4, using the 24 h post-injection serum t4 concentration in the clearance calculation. data from this laboratory indicate that serum t4 concentration is lowest at this point yielding falsely high clearance values, particularly when high doses of t4 are injected. to investigate this problem further, two types of experiments were performed. firs ... | 1976 | 947283 |
| paraplegia following transurethral surgery. | we have presented a case of paraplegia following spinal anaesthesia administered for a transurethral prostatic resection. further investigation revealed a metastatic peridural compressive lesion at the level of t4. we have discussed some of the contributory factors involved, chiefly the lumbar puncture (perimedullary vascular engorgement, alterations in c.s.f. dynamics) and the possible role of intra-operative water intoxication (swelling of spinal cord cells). | 1976 | 947506 |
| radioimmunoassay for 3,3',5'-triiodothyronine (reverse t3, r-t3) in unextracted human serum. | highly specific antibodies against 3,3',5'-triiodothyronine (reverse t3, r-t3) have been produced in rabbits. the crossreaction with t4 is about 0.05%. a radioimmunoassay for r-t3 in unextracted serum was developed. ans is used for blocking the binding of tracer and endogenous r-t3 to tbg. the sensitivity to the assay is 0.06 ng/ml plasma. the mean normal r-t3 concentration is 0.20 ng/ml. thyrotoxic patients show elevated levels; in most hypothyroid patients r-t3 concentrations are below the det ... | 1976 | 947601 |
| a simple and rapid thyroxine radioimmunoassy (t4-ria) in unextracted human serum; a comparison of t4-ria and t4 displacement assay, t4(d), in normal and pathologic sera. | a simple, rapid and accurate thyroxine radioimmunoassay (t4-ria) in unextracted serum or plasma has been described, and for comparison t4 determinations have also been made by a t4(d) procedure using abbott tetrasorb kits. t4-ria procedure basically involved denaturation of serum to dissociate t4-protein bond, and t4 released was allowed to react with [125i]t4-labeled t4 antiserum elicited by immunizing rabbits against bovine thyroglobulin. the displaced unbound [125i]t4 was rapidly taken up by ... | 1976 | 947623 |
| the specificity requirements of bacteriophage t4 lysozyme. | a series of bacterial cell wall glycopeptides of low molecular weight and cell wall nucleotide precursors have been tested for their inhibitory action on the digestion by t4 lysozyme of a radioactively labeled linear uncrosslinked peptidoglycan. the disaccharide-peptides glcnac-murnac-l-ala-d-glu(a2pm) (c5) and glcnac-murnac-l-ala-d-glu(a2pm-d-ala) (c6) as well as the monosaccharide-peptide murnac-l-ala-d-glu(a2pm) were found to be good competitive inhibitors (with similar ki values) whereas th ... | 1976 | 947753 |
| changes in serum triiodothyronine, thyroxine, and thyrotropin during treatment with thyroxine in severe primary hypothyroidism. | studies were performed on 40 patients with severe primary hypothyroidism, during treatment with varying doses of t4. therapy was initiated with 50 mug/day and was continued for at least 2 months. subsequent repeated increases of 25 mug/day were continued for at least 2 months until the serum tsh level was less than 10 muu/ml. measurements of serum t4 and t3, rt3u, and serum tsh were carried out at monthly intervals. trh tests were performed after tsh levels of less than 10 muu/ml had been attain ... | 1976 | 947930 |
| plasma thyroxine concentrations in ewes and their fetuses during the last six weeks of pregnancy. | changes in plasma thyroxine (t4) concentrations were followed in 27 fetal sheep after surgical implantation of catheters. fourteen days were required before stable concentrations of t4 were achieved, whether surgery was performed between 90 and 96 days or 109 and 120 days gestation. twenty-three fetuses were followed to birth, and during the last four days the t4 concentrations showed no change in 11 fetuses and a significant decrease in the other 12. birth occurred between 142 and 157 days gest ... | 1976 | 951516 |
| increased peroxidase activity in pendred's syndrome with hypothyroidism. | an 8-year-old boy with goiter and bilateral nerve deafness had a 46% discharge of radioiodine after thiocyanate administration. he was clinically euthyroid. although the serum total t4 was low (2.4 mug/100 ml) and tsh was significantly high (181 muu/ml), the serum total t3 was normal (152 ng/100ml). it was considered that the increased release of tsh by the feedback mechanism in response to the low t4 resulted in a quite normal level of serum t3. the thyroid gland demonstrated a low stable iodin ... | 1976 | 951708 |
| a screening test for thyroid function. | the achilles tendon reflex half relaxation time measurement (art) has been used by many physicians both as a diagnostic test and for the assessment of progress in thyroid gland malfunction. reference is made to some results obtained in melbourne and in other countries using different methods of measurement of the art for these purposes. in a series of 2064 patients referred to the shepherd foundation centre, the achilles tendon reflex half relaxation time was measured by means of the smi reflexo ... | 1976 | 952638 |
| triiodothyronine and thyroxine interrelationships in health and disease. | with the recent development of radioimmunoassay techniques for the measurement of serum triiodothyronine (t3) concentration, new concepts have arisen regarding the biologic role of t3 in health and disease and its interrelationships with thyroxine (t4). an awareness of the influence of clinical conditions that affect binding of thyroid hormone to plasma proteins is required in the interpretation of moderately increased or decreased serum t3 values. hormone preparations containing t3 may produce ... | 1976 | 953904 |
| correlation between structural transformation and cleavage of the major head protein of t4 bacteriophage. | we have studied the maturation of t4 polyheads, the aberrant tubular structures related to the capsid of t4 bacteriophage. conditions have been found under which more than 95% of the major head protein (p23) undergoes the same cleavage that occurs during development of the normal capsid. the concomitant structural changes in the polyheads have been followed using electron microscope image filtering techniques. as a result of the cleavage, a radical transformation of the hexagonal lattice occurs, ... | 1976 | 954078 |
| the assessment of 125i treatment of thyrotoxicosis. | 257 patients have been reviewed 1-5 years (mean 3 years 2 months) after receiving one of five dose regimes of 125i for thyrotoxicosis. the cumulative incidence of hypothyroidism was 34% and of persistent thyrotoxicosis 17%. the group receiving doses between 351 and 500 muci/g had the highest proportion of euthyroid patients (65%) with the lowest requirement for repeat therapy (46%). in the euthyroid patients, increasing dose of 125i was associated with progressive decline in mean thyroxine (t4) ... | 1976 | 954217 |
| characterization of t4 mutants that partially suppress the inability of t4rii to grow in lambda lysogens. | in the course of isolating viable t4 deletions that affect plaque morphology (homyk and weil 1974), two closely linked point mutant, sip1 and sip2, were obtained. they map between genes t and 52, cause a reduction in plaque size and burst size, and partially suppress the lethality of rii mutants for growth in lambda lysogens. the characteristics demonstrate that sip1 and sip2 are similar to mutants previously reported by freedman and brenner(1972). in addition, d. hall (personal communication) h ... | 1976 | 955402 |
| bacterial characteristics determining the potential host range of bacteriophage t4. | many members of the enterobacteriaceae, but not other gram-negative organisms, apparently carry a specific recognition site for the t4 tail tube on their cytoplasmic membranes. | 1976 | 956114 |
| control of mutation frequency by bacteriophage t4 dna polymerase. i. the cb120 antimutator dna polymerase is defective in strand displacement. | the ts cb1200 (antimutator) mutation in bacteriophage t4 dna polymerase increases the accuracy of dna replication since it results in a decrease in the frequency of mutations in other phage genes. the cb120 polymerases differs from the wild type enzyme in the slow rate at which it copies templates where primer extension requries displacement of polynucleotides base-paired to the template strand, even in the presence of the t4 dna unwinding protein (gene 32-protein). the ratio of nucleotides turn ... | 1976 | 956182 |
| control of mutation frequency by bacteriophage t4 dna polymerase. ii. accuracy of nucleotide selection by the l88 mutator, cb120 antimutator, and wild type phage t4 dna polymerases. | the accuracy of nucleotide selection by wild type, l88 mutator, and cb120 antimutator t4 dna polymerases has been compared by measuring both stable incorporation of complementary and noncomplementary nucleotides into polymer and the dna-dependent conversion of deoxynucleoside triphosphate to monophosphate. the increased accuracy of the cb120 antimutator enzyme is shown by a ratio of utilization of incorrect to correct nucleotides with poly(da)-poly(dt) as template which is only 10 to 30% of that ... | 1976 | 956183 |
| plasma thyrotropin, thyroxine, and triiodothyronine relationships in man. | the physiologic relationships of plasma tsh, t4 and t3 levels measured every 20 min in seven healthy young men and one healthy young woman have been investigated. a nocturnal tsh surge was observed in all subjects on both nights of the 36-48 h baseline observation period. in males the maximum plasma tsh value occurred at 2300 h. the mean peak tsh level was 2.0 +/- 0.3 (se) muu/ml compared with a mean of 1.3 +/- 0.9 muu/ml for the entire baseline records of the 8 subjects. the effect of iv infusi ... | 1976 | 956341 |
| thyroid-pituitary feedback during iodine repletion. | the changes in serum triiodothyronine (t3), thyroxine (t4), and thyrotropin (tsh) were measured during iodine repletion in a woman who was severely iodine-deficient because of a congenital iodide-trapping defect. serum t3 became detectable 12 h after iodine was begun and reached 68 ng/dl, a level within the normal range, by 36 h. it rose progressively during the first 9 days reaching a supra-normal level (200 ng/dl) and then fell slowly to 130 ng/dl. serum t4 was not detected (less than 1 mug/dl ... | 1976 | 956345 |
| thyroid function in neonatal hypothyroidism. | various aspects of the thyroid function have been measured in 28 cases of neonatal hypothyroidism detected by means of the quebec screening program for metabolic diseases. in all instances the t4 value in the blood of filter paper spot was below 2 sd of the mean of the day, averaging 0.39 +/- 0.04 ng/40 mul (mean +/- sem) of eluted blood. the t4 value of a second similar sample averaged 0.22 +/- 0.04 ng/mul of eluted blood; this value was significantly lower than the first one. the serum t4 conc ... | 1976 | 956995 |
| tsh measurements from blood spots on filter paper: a confirmatory screening test for neonatal hypothyroidism. | a sensitive radioimmunoassay for the measurement of tsh in the eluate of blood spotted on filter paper has been developed. the method is consistently sensitive to 0.1 to 0.25 muu of tsh and enables the detection of values equivalent to 6 to 15 muu/ml of serum. the measurement of tsh in the filter paper spot in all infants with low filter paper spot t4 has permitted rapid confirmation of 10 cases of neonatal hypothyroidism. however, cases of hypothalamic hypothyroidism with low or normal filter p ... | 1976 | 956996 |
| head length determination in bacteriophage t4: the role of the core protein p22. | 1976 | 957424 | |
| mutator mutations in bacteriophage t4 gene 32 (dna unwinding protein). | bacteriophage t4 gene 32 encodes a dna unwinding protein required for dna replication, repair, and recombination. gene 32 temperature-sensitive mutations enhance virtually all base pair substitution mutation rates. | 1976 | 957480 |
| utilization of early promotors in mutant far p85 of bacteriophage t4. | we show that farp85 is a recessive mutant of t4 incapable of activating the delayed early promotors for genes 43 and 45 and that the farp85 mutation is in the same complementation group as the ts g1 mutation, which is located in the "modifier of transcription" (mot) gene. | 1976 | 957485 |
| plasma catecholamine concentrations in hyperthyroidism and hypothyroidism. | using a modification of the fluorometric method of anton and sayre, we have measured the plasma epinephrine (e) and norepinephrine (ne) concentrations in patients with thyroid dysfunction. there was no significant difference in plasma e in hyperthyroid or hypothyroid subjects, the values being similar to those observed in normal subjects. there was a striking relationship between age and plasma ne in the euthyroid individuals (r = 0.685, p less than 0.001, n = 41). observed plasma ne concentrati ... | 1976 | 958003 |
| studies on obesity. iii. effect of triiodothyronine (t3) on thyroglobulin autoantibodies in euthyroid obese subjects. | effect of t3 therapy on tanned red cell agglutinating thyroglobulin (trc-tg) antibodies in 10 obese subjects without apparent thyroid disease was investigated. six other obese subjects without thyroid dysfunction and of approximately the same mean age who also had circulating trc-tg antibodies served as control subjects and were untreated. in vitro thyroid tests (tsh, total and free t4) performed before t3 therapy, as well as clinical examination, showed thyroid function to be normal in all subj ... | 1976 | 958004 |
| [thyroid hormones and thyrotropin in liver and kidney insufficiency]. | in 22 patients with hepatic or renal insufficiency the serum concentrations of trijodothyronin, thyroxine and thyrotropin and also the t4-binding capacity of tbg were determined. the mean serum t3 concentration was found to be significantly lower in patients with hepatic coma when compared with euthyroid subjects. in the cases of renal insufficiency the serum t3 concentrations were in the normal range. due to hormone loss through dialysis however, the mean value of the t3 concentrations was slig ... | 1976 | 958900 |
| pathways of dna repair in t4 phage. ii. sedimentation analysis of intracellular dna in repair-defective mutants. | 1976 | 960557 | |
| subcellular membrane fatty acids of rat heart after cold acclimation or thyroxine. | relative percent changes in fatty acid (fa) composition of heart mitochondria and microsomes from 30-day-warm-acclimated and cold-acclimated rats were determined by gas chromatography. rats in both environmental groups received thyroxine (t4) (1 mg/kg per day) or propylthiouracil (ptu) (5 mg/rat per day) for 10 days prior to sacrifice. mitochondrial total unsaturated fatty acids (tufa) decreased in cold-acclimated rats relative to warm-acclimated controls but not in cold-acclimated animals recei ... | 1976 | 961919 |
| effects of 3'-fluorothymidine, nalidixic acid, and mitomycin c on temperature-sensitive mutants of the dna polymerase of phage t4. | the temperature-sensitive dna polymerase mutator mutants a58 and l98 are less inhibited by 3'-fluorothymidine than the t4 wild type and the antimutator mutant cb121, in consequence of the assumed differences between the polymerase-associated exonuclease activities. this interpretation is confirmed by results with nalidixic acid and mitomycin c. the use of systems of different temperature-sensitive mutants of one or more genes is proposed for 1.) investigating the mode of action of drugs, 2.) st ... | 1976 | 969568 |
| goiter formation following prostaglandin administration in rats. | prostaglandins (pge1 and pge2) induced a hyperplastic microfollicular goiter with a high radioiodine (131i) thyroid uptake, increased endocytosis, a heavy autoradiographic (125i) reaction, and a moderate increase of thyroid hormones (t4, t3), thyroxine-binding globulin (tgb), and thyrotropin (tsh) concentrations in adult rats. ultrastructurally, both prostaglandins (e1 and e2) markedly stimulated the thyroid cell activity and increased the number of pseudopodia, the size of colloid and dense gra ... | 1976 | 970439 |
| structure of t4 polyheads. ii. a pathway of polyhead transformation as a model for t4 capsid maturation. | 1976 | 972397 | |
| replication of t4 dna in vitro. ii. assay system for and some properties of gene products required for t4 dna replication. | [3h]dttp was not incorporated into t4 dna in the in vitro system for t4 dna replication when the system was prepared from cells infected with t4 amber mutants defective in dna replication. [3h]dttp incorporation was resumed by adding the missing gene product to the defective system. dna replication by the reconstituted system proceeded by the discontinuous mode of replication, as observed in the wild-type system. by using this in vitro complementation system, molecular weights of gene 41, 43, 44 ... | 1976 | 972431 |
| the thyroidal response to chronic goitrogenic stimulation and the persistence of effects of early goitrogenic stimulation. | the effects of overstimulation of the thyroid gland induced by the feeding or rapeseed meal or thiouracil during the growing period persisted for as long as 75 weeks after withdrawal of the source of goitrogen from the diet. thyroid weight, thyroid iodine content and radioiodine uptake were all increased in birds which had received goitrogen during the growing period. follicle diameter was greater and the height of epithelial cells was lower than in control birds. the thyroid changes in these bi ... | 1976 | 974885 |
| optimization of t assay: a model study. | factors involved in the optimization of competitive binding radioassays have been analyzed using a thyroxine (t4) radioassay as a model system. the effects of kinetic errors are minimized by the use of the lowest possible incubation temperature for the separation step and by the use of labeled t4 of high purity (which is not consistently available from commerical sources). the use of labeled t4 containing significant quantities of labeled triiodothyronine (t3) can lead to a marked increase in bi ... | 1976 | 975571 |
| effect of fatty acids on thyroid function tests in vitro and in vivo. | addition of long-chain fatty acids to serum increased thyroxine (t4), measured by a competitive protein binding assay, and triiodothyronine (t3) uptake by sephadex or resin (t3u tests). this is compatible with the assumption that fatty acids compete with thyroxine for binding sites on t4-binding proteins. when equimolar concentrations of various saturated and unsaturated fatty acids were added to serum it was observed that the effectiveness in raising tests based on protein binding of thyroid ho ... | 1976 | 975583 |
| pharmacological influences on t4 to t3 conversion in rat liver. | five groups of 8 rats were treated with normal diet, diphenylhydantoin, dexamethasone, thyroxine (t4) and phenobarbital respectively for 14 days. after this period plasma triiodothyronine (t3), t4 and reverse t3 (rt3) concentrations as well as the t4 to t3 converting activity in the liver homogenates were determined. plasma t3 was low after dexamethasone, t4 and phenobarbital. plasma t4 was low after dexamethasone and elevated after t4. rt3 plasma concentrations were at the lower limit of detect ... | 1976 | 975586 |
| pituitary thyrotropin (tsh) rebound phenomenon and kinetics of secretion in the goitrous rat: differential effects of thyroxine on synthesis and release of tsh. | the protein metabolism and [3h]-uridine uptake of thyroid and adenohypophysis and the kinetics of pituitary tsh rebound (ptr) were studied in goitrous female rats (fed propylthiouracil, ptu: for 7-12 weeks) following single, iv injections of l-thyroxine (t4: 0.8 to 200 mug). goitrogenesis was associated with reduced protein concentration and enhanced uptake of [3h] uridine in both glands. plasma levels of tsh were invariably elevated but stores in the adenohypophysis were consistently reduced. s ... | 1976 | 976195 |
| compensatory thyroid hypertrophy after hemithyroidectomy in rats. | thyroid enlargement occurs in association with a variety of circumstances characterized by an impaired capacity of the gland to secrete adequate amounts of hormone. to elucidate the factors responsible for such compensatory thyroid growth, particularly the role of tsh, we have observed the response of the serum tsh, t3 and t4 concentrations following hemithyroidectomy in the rat, and have attempted to correlate changes in these functions with changes in the weight and histology of the thyroid re ... | 1976 | 976198 |
| a comparison of the effect of propylthiouracil on the respiration of thyroid slices from humans, chickens and rats. | 1. the qo2 of thyroid slices from hyperthyroid patients treated with propylthiouracil (ptu) was 140% that of slices from euthyroid patients. similar results were obtained using thyroid slices from ptu fed and control chickens. 2. ptu feeding caused thyroid hyperplasia, increased qo2 more than could be accounted for by the hyperplasia but neither increased qo2 of muscle, cerebral cortex or spleen nor altered serum t4 levels. 3. ptu in drinking water increased the size and vascularity of thyroids ... | 1976 | 976744 |
| dose-response of prolactin and thyrotropin to n3im-methyl-thyrotropin releasing hormone in euthyroid men. | the synthetic n3im-methyl analogue of thyrotropin releasing hormone (methyl-trh) was administered intravenously to 15 euthyroid men, ages 36-62, in graded doses from 6.25 mug to 500 mug in order to establish the range of response of prolactin (prl), tsh, t3 and t4 to various doses of methyl-trh. there was a dose-related rise in serum tsh, prl, t3, and t4 which gave a nearly linear relationship when the integrated area of response was used as an index of response to the various doses of methyl-tr ... | 1976 | 977724 |
| radioimmunoassay kits for thyroid hormones. | characteristics of the t4 and t3 radioimmunoassay kits that are commercially available in the uk are described. useful comparative data and criticism are given about many of these kits. some unacceptable variation in accuracy is demonstrated. | 1976 | 977766 |
| serum triiodothyronine determination in clinical use. | two radioimmunassays for the determination of serum triiodothyronine (t3) were developed. the assay of t3 in unextracted serum had several advantages over the assay on extracted serum and was chosen for the routine determination of t3 in serum from 117 patients requiring assessment of their thyroid status. in 53 subjects considered retrospectively not to have thyroid dysfunction nor to have been on steroid contraceptives or therapy, the pooled mean serum t3 concentration was 1-92 (actual range 0 ... | 1976 | 977774 |
| studies of the structure of the t4 bacteriophage tail sheath. i. the recovery of three-dimensional structural information from the extended sheath. | 1976 | 978722 | |
| role of genes 46 and 47 in bacteriophage t4 reproduction. iii. formation of joint molecules in biparental recombination. | 1976 | 978724 | |
| [successful treatment of thyroid storm by continuous plasmapheresis with a blood-cell separator (author's transl)]. | continuous plasmapheresis with the ibm blood cell separator showed to be a successful therapy in a 26 year old patient with thyroid storm. the patient regained consciousness already during plasmapheresis whereby 5 liters of plasma were exchanged within 3.5 h. although 633 mug thyroxine (t4) and 13.6 mug triiodothyronine (t3) were removed with the 51 plasma, no fall occurred in total serum t4 and t3 levels, probably due to the rapid reflux of thyroid hormones from the tissue into the intravascula ... | 1976 | 979079 |
| the dependence of hno2 mutagenesis in phage t4 on ligase and the lack of dependence of 2ap mutagenesis on repair functions. | a temperature sensitive ligase allele of phage t4 reduced or eliminated hno2 induced reversion of am mutants. since at the temperatures used, the ligase mutant is defective in the repair of some types of lethal lesions (i.e., uv, mms and ems induced lesions) these results indicate that hno2 mutagenesis may occur through a ligase dependent repair pathway. in contrast, 2ap induced mutation was not inhibited by mutants defective in the gene 30 ligase or in genes 32, 39, 41, 42, 44, 45, 46, 47, 49, ... | 1976 | 979960 |
| serum t3 and t4 levels in sick children. | 1976 | 980611 | |
| freezing injury in bacteriophage t4. | 1976 | 981243 | |
| radioimmunoassay for serum thyroxine. | a new procedure for radioimmunoassay (ria) was applied to determine the serum concentration of thyroxine (t4). mixtures of t4-methylester hydrochloride-bovine serum albumin complex and complete freund's adjuvant were injected to rabbits to get them immunized. specific anti-t4 rabbit serum, high in titer, could be obtained 18 months after the start of immunization. ria for t4 extracted by ethanol from serum was performed by an application of dextran-coated charcoal to separate bound and free 125- ... | 1976 | 982432 |
| morphologically recognizable markers for scanning immunoelectron microscopy. ii. an indirect method using t4 and tmv. | 1976 | 982828 | |
| recurrent goiter, hyperthyroidism, galactorrhea and amenorrhea due to a thyrotropin and prolactin-producing pituitary tumor. | a 22-year-old woman with recurrent goiter, hyperthyroidism, galactorrhea, and amenorrhea due to a pituitary tumor is described. she had been treated surgically twice for recurrent goiter with tracheal compression. despite clinical signs of hyperthyroidism and slightly elevated plasma thyroid hormone levels (t4: 11 mug/dl; t3: 189 ng/dl), without thyroid hormone replacement therapy the basal tsh level was elevated up to 23 muu/ml and could not be suppressed by exogenous thyroid hormones: even whe ... | 1976 | 985824 |
| diural tsh variations in hypothyroidism. | there is a circadian variation in serum tsh in euthyroid subjects. a similar diurnal variation has been demonstrated in patients with hypothyroidism. in the present study the 24-hour pattern of serum tsh was investigated in eight patients with hypothyroidism of varying severity and in five hypothyroid patients treated with thyroxine (t4). there was a circadian variation in serum tsh in patients with hypothyroidism of moderate degree, and in patients treated for severe hypothyrodism with thyroxin ... | 1976 | 985825 |
| [tsh stimulation test using serum t3 and t4 as indicators]. | 1976 | 986885 | |
| [reproducibility of emesis by orally administrated copper sulfate in cats]. | adult cats were administered oral threshold doses of copper sulfate every week. as the cats vomited in 70 out out 80 cases, the reproducibility was 88%. peripheral vomiting threshold dose was 40 mg/head or less, while the threshold dose for oral copper sulfate emesis after t4 transection and vagotomy was more than 160 mg/head. the following method is thus proposed for application in evaluating an antimetic for oral copper sulfate. adult cats are to be given the emetic once a week. the threshold ... | 1976 | 986987 |