Publications

protection of parental t4 dna from a restriction exonuclease by the product of gene 2.		1977	592405
suppression of gene 49 mutations of bacteriophage t4 by a second mutation in gene x: structure of pseudorevertant dna.	mutations in gene 49 of bacteriophage t4 were suppressed by a second mutation in gene x. mapping studies located gene x between genes 41 and 42. complementation results indicated that mutations in fdsa gene (a suppressor of gene 49 mutants) were in gene x. the intracellular pseudorevertant dna was examined for unusual properties which could explain its successful encapsidation. after the in vivo inactivation of a temperature-sensitive gene 32 (dna unwinding) protein, the intracellular pseudoreve ...	1977	592466
age related changes in serum throxine and 125i-triiodothyronine resin sponge uptake in the young dog.	serum thyroxine (t4) concentrations and 125i-labelled triiodothyronine resin sponge uptakes (t3-rsu) were determined in young dogs every 10 days between 3 and 103 days of age. when compared to the adult mean of 1.7 microgram t4/100 ml serum, the t4 in young dogs was elevated throughout the ages studied. the serum t4 concentration was twice the adult value at 3 days of age, rose to over five times the adult value in the 1st month of life, and declined again to twice the adult mean by 103 days of ...	1977	592719
the radioimmunological measurement of thyroglobulin secretion in vitro under the influence of some herbicides.	a radioimmunoassay (ria) technique of human thyroglobulin (tgl) was developed using 125i-tgl and a rabbit anti-tgl serum at the initial dilution of 1:100,000. the sensitivity of the technique was of 2.5 ng/ml. the ria of tgl was applied as a screening test for the in vitro secretory activity of human thyroid cells under the influence of two herbicides of the heterocyclic group (amitrol and atrazin). three levels of concentration of amitrol and atrazin were administered to the tissue culture tube ...	1977	594626
[cryosurgical treatment of prostate gland cancer].	the authors share their experience with cryosurgical treatment of 38 patients with prostatic cancer (stage t3--in 9 patients, t4--in 29). the technic of cryotherapy is described. the former was employed in patients with complete obstruction of the cervico-urethral segment being nonresponsive to the hormone- and radiotherapy, and also for a life cystostome. three freezing sessions for 5--15 minutes were conducted with natural warming of the prostate between sessions during 10--12 minutes, and for ...	1977	595515
the effect of body temperature on thyroid hormone levels in patients with non-thyroidal illness.	during studies on the mechanism underlying the low serum t3 levels in euthyroid patients with various acute and chronic non-thyroidal illnesses, it became evident that body temperature may be one parameter associated with changes in serum t3 levels. forty-nine hospitalized, euthyroid patients with hyperpyrexia caused by various non-thyroidal illnesses were studied. the levels of serum t3 were found to decrease gradually with increasing body temperature. t3 was already below the normal level +/- ...	1977	596246
thyroid hormone kinetics: improved method for quantitative separation and measurement of the various radioiodothyronine injection.	an accurate and reproducible method for measurement of radioactive species in blood after in vivo injection of labelled iodothyronines is described. by extraction with high-affinity antisera, radioactive reverse t3 and t3 are separated from serum quantitatively. radioiodide is quantitatively separated from radio-thyronine species and serum proteins by sephadex g50 filtration. the residual mixture of radio-t4 and iodoprotein is quantitatively resolved by ion-exchange adsorption. minimal misclassi ...	1977	598013
joining of synthetic ribotrinucleotides with defined sequences catalyzed by t4 rna ligase.	rna ligase catalyzed the joining of pc-c-ap with c-a-a in the synthesis of c-a-a-c-c-ap, which has the sequence of the escherichia coli trnafmet 3'-end. pc-c-a was also shown to be joined to c-a-a without any undesired self-polymerization. joining of pc-c-a to various synthetic ribotriplets, such as c-c-a, a-a-a, c-c-c, u-u-u, u-a-g, c-c-g and u-u-c, was performed as well as joining to the partially substituted trimers with a photolabile o-nitrobenzyl group, c-anbzl-a and c-c-anbzl. the yields w ...	1977	598370
effect of insulin on thyroid hormone blood levels in normal and thyroidectomized dogs.	administration of insulin brings about an increase of thyroid hormone blood concentration. this study investigated the possibilities that the elevation is due to increased thyroid secretion or to liberation of hormone from hepatic stores. monocomponent insulin was administered to intact dogs by jugular perfusion (25 mu/kg/min. for 15 min.) and to thyroidectomized dogs either as single dose i.m. (1 u/kg) or by jugular perfusion (as above). all animals showed at least halving of blood glucose conc ...	1977	598564
action of t4 endonuclease v on dna containing various photoproducts.	the action of t4 endonuclease v on dna containing various photoproducts was investigated. (1) the enzyme introduced strand breaks in dna from ultraviolet-irradiated vegetative cells of bacillus subtilis but not in dna from irradiated spores of the same organism. dna irradiated with long wavelength (360 nm peak) ultraviolet light in the presence of 4,5',8-trimethylpsoralen was not attacked by the enzyme. these results indicate that 5-thyminyl 5,6-dihydrothymine (spore photoproduct) and psoralen m ...	1977	599143
a mutation which bypasses the requirement for p24 in bacteriophage t4 capsid morphogenesis.		1977	599558
studies related to the head-maturation pathway of bacteriophages t4 and t2:i. morphology and kinetics of intracellular particles produced by mutants in the maturation genes.	mutants in the genes governing the maturation of the head of bacteriophage t4 and in gene 24 were studied by electron microscopy of thin sections. we define morphologically: black particles, comprising mature, stable heads and immature, fragile heads, which break down upon lysis; grizzled particles, which apparently are partially filled or partially emptied; empty large particles without dna or core which are all the same size as normal heads; empty small particles without dna and without core w ...	1977	599933
studies related to the head-maturation pathway of bacteriophages t4 and t2:ii. nuclear disruption, protein synthesis and particle formation with the mutant 43-.30-.46-.	we describe the aberrant phage multiplication of the triple conditional lethal mutant 43-(polymerase).30-(ligase).46-(exonuclease) of bacteriophage t4d in which phage dna replication is arrested but some late protein synthesis occurs (33). the nuclear disruption is indistinguishable from wild type. forty-five empty small and empty large particles are assembled per cell when the multiplicity of infection (m.o.i.) is 100. this number corresponds closely to the 38 phage equivalents of cleaved major ...	1977	599934
molecular cloning of fragments of bacteriophage t4 dna.	non-glucosylated t4 dna was digested with r.ecori and the resulting fragments covalently joined to lambda vectors. the genetic content of each lambda-t4 hybrid was determined by marker-rescue tests. the isolation of many recombinants containing partial-digestion products of t4 dna provided the overlapping sequences necessary to order fragments within the t4 genome. the present analyses include parts of the "early" region between genes 42 and 46, and much of the "late" region between genes 50 and ...	1977	600265
radioimmunoassay of thyroxine and triiodothyronine in urine using extraction and separation of sephadex columns.	radioimmunological methods for the determination of thyroxine (t4) and triiodothyronine (t3) in urine have been developed. the methods are based on trapping of t4 and t3 from unextracted urine, followed by separation between free and antibody bound hormone on the same sephadex column. the t4 method has been compared to a competitive protein binding (cpb) assay using ethyl acetate extraction. the methods are evaluated in sixty-seven euthyroid controls, twenty-four hyperthyroid and seven hypothyro ...	1977	601507
radioimmunological determination of reverse triiodothyronine in unextracted serum and serum dialysates.	a sensitive radioimmunoassay for measurements of 3,3'-5'-triiodothyronine (reverse t3, rt3) in small amounts of unextracted serum is described. the interference of rt3 binding proteins in serum was precluded by addition of 8-anilino-1-naphthalene sulphonic acid (ans). the cross reaction of t4 with the rt3 anti-serum varied with the concentration of t4 in the samples. at 50 per cent inhibition of [125i]rt3 binding, the t4 cross reaction was 0.075% (mol/mol). all values were corrected for t4 cross ...	1977	601517
[demonstration of an agglutination-like nature of haptoglobins against streptococci with t4-antigen through the examination of umbilical cord sera].	the determinability of haptoglobin types is correlated with the titers of agglutination against streptococci carrying the t4 antigen. if the haptoglobin types in cord-sera cannot be determined (as a result of the physiological binding of haptoglobins) the agglutination reaction fails or the titers are greatly diminished. the dimorphism, which was found in sera of adults, can be demonstrated in cord sera, too, if they can be typed. it is concluded that the observed reaction between hp 2-2 and hp ...	1977	602509
procedures for characterisation of the genetic material of candidate vaccine strains.	two procedures for characterising the genomes of recombinant influenza viruses are described. the first of these involves ribonuclease t4 oligonucleotide fingerpart analysis of separated viral rnas labelled either in vivo or in vitro and the second utilises polyacrylamide gel electrophoresis to identify the double-stranded molecules formed by hybridisation between the complementary and virion rnas of two viruses. although the latter method is more suitable for routine screening purposes, both pr ...	1977	604097
serum hormones in the perinatal pig and the effect of exogenous insulin on blood sugars.	growth hormone (gh), insulin, cortisol, triiodothyronine (t3) and thyroxine (t4) were determined in serum of fetal pigs at 90, 100, 110 and 114 days of gestation and of newborn pigs at 1 and 24 hours of age. fetal gh, t3 and cortisol increased between 90 and 110 days. gh concentration decreased between 110 and 114 days, while t3 and cortisol were unchanged. t4 was variable in fetal pigs and no relationship with t3 levels was apparent. fetal insulin was constant at approximately 10 muu/ml. in new ...	1977	604183
evaluation of thyroid functions in leprosy. i. thyroid function tests.	twenty six patients of different types of leprosy were studied for radio active iodine uptake (i131) and serum levels of triiodothyronine (t3), thyroxine (t4) and thyroid stimulating hormone (tsh). none of the patients had clinical evidence of thyroid involvement. no significant difference was found between the values obtained in patients and normals and in different varieties of leprosy.	1977	604656
[clinical prognostic factors in breast cancer].	five year survival of 683 mammary carcinomas would confirm the prognostic value of t.n.m. classification (uicc), of tumor particular characteristics (size, fixation to neighbouring structures) and presence or absence of clinical nodes. a clinical-histological relationship was studied for axillary nodes: in our experience, clinical determination is more reliable for estimation of n0 and n2 than n1. three groups of age were considered; the age, t and n corrected five year survival show a higher mo ...	1977	607998
acute decrease of blood thyroid hormone by isovolemic exchange transfusion as a stimulus for pulse tsh release and its modifications by cns lesions.	the level of thyroid hormone in blood has been acutely decreased by about 20--30% within 20 min with the aid of isovolemic exchange transfusion (iet) of thyroid hormone free blood cell suspension (thfbcs) in three groups of rats: (1) intact control (c); (2) median eminence (me)-lesioned; (3) thalamus (th)-lesioned. the levels of thyroxine (t4) and tsh were measured by specific radioimmunoassay (ria) before the transfusion and for 180 min after that. the level of t4 increased to the initial value ...	1977	609370
[role of thymine dimers in the uv mutagenesis of bacteriophage sd].	the sensitized irradiation (lambda greater than 310 nm) with acphim induces plaque mutants of extracellular phage sd. thus, tt in phage sd dna are both lethal and mutagenic. the yield of plaque mutants is about 3-fold lower as compared with the yield after uv-irradiation (lambda = 254 nm). the theoretical analysis of uv-induced mutagenesis curves of phage sd was carried out. it is concluded that the following photoproducts make a contribution in the mutagenic effect of near-uv (lambda = 254 nm): ...	1977	612486
some changes in the composition of blood during the first 24 hours after birth in normal and growth retarded lambs.	changes in the composition of blood which occurred during the first 24 hr after birth were examined in 18 lambs whose prenatal growth had been reduced by limiting the size of the placenta (group ce) and in 18 lambs born to untreated ewes (group r). the gestational period was about 4.5 days shorter in group ce than in group r and the birth-weights of group ce lambs were reduced by about 0.5 kg. at birth the pcv and the plasma concentrations of glucose were significantly higher and the plasma conc ...	1977	615517
primary humoral immune response against tumor membrane antigens and foreign antigens by plasma cell tumor bearer mice.	the mopc-460 plasma cell tumor possesses tumor specific antigens. it nevertheless grows and kills its syngeneic host. the possible impairment of immune responsiveness in tumor bearing mice was investigated by measuring their ability to mount a humoral immune response against foreign antigens, such as sheep red blood cells or bacteriophage t4. no significant decrease in the response to either antigen was found until the tumor mass exceeded 10-15% of the host's body weight. moreover, circulating a ...	1977	615743
a critical analysis of some thyroid function tests.	the results of a comparative study of thyroid function tests are reported, the assay for pbi having been used for reference. the merits and sources of error of the bio-rad column test, thyopac-4 test and t3-ria test are discussed with reference to pbi. the correlation coefficients and the sources of error being taken into consideration, pbi represents a fairly reliable indicator of t4 values and recommends itself on these grounds as a basic routine procedure, the more so as it is simple, cheap a ...	1977	617927
early detection of neonatal hypothyroidism by serial tsh determination in dried blood. six months experience with a reliable, efficient and inexpensive method.	mass newborn screening for primary hypothyroidism was introduced in switzerland on january 1st, 1977, using a radioimmunoassay of tsh in dried blood spotted on filter paper. after incubation for 38 h at 20 degrees c, bound and free tsh is separated by double antibody precipitation. the filter paper discs of 6.5 mm diameter remain in the test tubes. at present, one tsh determination costs approx. sfr. 4.40. all reagents used are commercially available and their costs amount to not more than 15% o ...	1977	617976
drug interference with laboratory tests: oral contraceptives.	changes in serum protein levels are produced by oral contraceptives. they reflect hepatic synthesis of proteins. changes range from a 40% decrease in orosomucoid to an 180% increase in ceruloplasmin. alterations in the concentration of some globulin proteins lead to altered serum levels of some hormones and trace metals. plasma levels of cortisol and thyroxine are most affected. an estrogen-induced increase in thyroxine-binding globulin causes a transient reduction in free thyroxine (t4). ...	1977	12260155
evidence for nuclear sites of stereospecific opiate binding in neuroblastoma cells in continuous culture.	a class of stereospecific, saturable receptors for narcotic drugs has been found in a clone of mouse neuroblastoma cells in continuous culture. cells grown in the presence of 10(-8) m etorphine, a synthetic opiate, had an increased doubling time. the neuroblastoma cell nucleus was found to be the sole site for the high affinity binding of etorphine. it was found that these nuclear receptors (1) became saturated at a concentration of 2 x 10(-9) m etorphine, (2) had a dissociation constant of 5 x ...	1976	196299
nucleotide sequences at the 5'termini of rabbit alpha and beta globin mrna.	the nucleotide sequences at the 5' termini of rabbit alpha and beta globin mrnas have been determined. periodate oxidation of globin mrna followed by reduction with 3h-sodium borohydride and subsequent analysis of the 3h-labeled mrna reveals the presence of the "cap" structure m7g5'ppp- blocking the 5' terminus. after periodate oxidation, beta elimination, and phosphomonoesterase treatment to remove the m7g5'ppp- "cap," the 5' end of globin mrna was labeled with 32p using gamma-32p-atp and t4 po ...	1976	189943
partial purification and properties of a dna-binding protein from nuclei of cells infected with polyoma virus.	a dna-binding protein has been purified from nuclei of 3t3 cells infected with polyoma virus. the assay used to detect this activity measures the amount of double-stranded dna retained on a nitrocellulose membrane filter in the presence of binding protein. the interaction between dna and protein is salt dependent and occurs optimally at 0.8 m nacl. the isolated protein can bind to both circular and linear duplex dna. incubation of the binding protein with pm2 or polyoma dna results in the format ...	1976	174725
kinetic studies on the reaction catalyzed by polynucleotide kinase from phage t4-infected escherichia coli.	kinetic properties of polynucleotide kinase (ec 2.7.1.78) isolated from escherichia coli cells infected with phage t4 were investigated. the reaction depends on the concentration of mgatp, while free atp or free mg2+ have neither inhibitory nor accelerating effect. the initial reaction velocity was plotted against variable concentrations of atp as the phosphate donor at various fixed concentrations of 5'-hydroxyl-dna or -oligo(ra) as the phosphate acceptor in the presence or absence of products. ...	1976	174738
total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer rna from escherichia coli. 12. synthesis of a dna duplex corresponding to a sequence of 23 nucleotide units adjoining the c-c-a end.	in continuing the work on the total synthesis of the gene for an escherichia coli tyrosine suppressor trna (accompanying papers) and as a part of a study of the mechanism of transcription of this gene, a 23-nucleotide unit-long dna corresponding to the previously determined (loewen, p., sekiya, t., and khorana, h. g. (1974) j. biol. chem. 249, 217) sequence has been synthesized. the synthesis was carried out by dividing the total duplex into the following five deoxyribooligonucleotide segments, ...	1976	175059
altered thyroidal responsivity to thyrotropin induced by circulating thyroid hormones. a "short-loop" regulatory mechanism?	we studied the effect of thyroid hormone administration on responsivity of murine thyroid to exogenous thyrotropin (tsh) in order to explore the possibility that the thyroid gland might be directly inhibited by its own hormones. in the rat both l-thyroxine (t4) and 3,5,3'-l-triiodothyronine (t3) pretreatment inhibited tsh-induced thyroidal ornithine decarboxylase (odc) activity in vivo in a dose-related manner (half-maximal inhibition, 1.7 mug/rat and 0.6 mug/rat, respectively). other structural ...	1976	175094
level of specific prereplicative mrna's during bacteriophage t4 rega-, 43- and t4 43- infection of escherichia coli b.	the role of the t4 bacteriophage rega gene in stabilizing early mrna was investigated by assaying the level of functional mrna from eight prereplicative genes (56 [dcmp hydroxymethylase], cd [dcmp deaminase], 1 [deoxynucleotide kinase], riia, riib, 46 [dna arrest], and 45) during extended infection of escherichia coli b with t4 rega-, 43- and t4 43- bacteriophage. the above gene-specific transcripts in rna isolated from infected cells were quantitated by translation with an e. coli b cell-free s ...	1976	176430
simian virus 40 dna replication: characterization of gaps in the termination region.	a class of precursor dna (pdna) ii molecules has been identified as the immediate precursor of simian virus 40 dna i. a pdna ii molecule contains a strand of newly synthesized dna with an interruption located in the region where dna synthesis terminates (4). these pdna ii molecules have been isolated and further characterized. they are converted to covalently closed structures (simian virus 40 dna i) only when they are treated in vitro with both t4 dna polymerase and escherichia coli ligase. aft ...	1976	176434
protein synthesis in bacteriophage ghost-infected cells.	escherichia coli b infected with t4 phage ghosts at 10 mm mg2+ regains its protein synthesizing activity upon addition of atp, gtp, and their generator to approximately 2% of the intact exponentially growing cells. in contrast to amino acid incorporation by intact cells, this system is sensitive to edta or low mg2+. on the other hand, this system, differing from the regular cell-free system, does not respond to addition of soluble protein and ribonuclease. the ghost-infected cells were able to s ...	1976	176455
temperature dependent changes in the thyroid gland of mertensiella caucasica (urodela, amphibia).	the influence of temperature on the thyroid gland of the caucasian salamander, mertensiella caucasica has been studied (1) with the light- and electron microscopes, (2) with enzyme histochemical and quantitative methods. animals which had been kept for years at 16 degrees c were exposed for 48 hrs to 4 degrees c or 27 degrees c. at 4 degrees c the follicular epithelium consisted of cuboidal cells with well-developed rough er, occasional distended cisternae of the rough endoplasmic reticulum (er) ...	1976	177213
pituitary-thyroid function in trophoblastic disease.	pituitary-thyroid function was assessed in 12 patients with trophoblastic disease (4 hydatidiform mole, 3 invasive mole, and 5 choriocarcinoma). thyroid-stimulating activity was detectable, by means of the mckenzie bioassay, in 6 patients (group 1) but not in the other 6 patients (group 2). in group 1 serum thyrotropin (tsh) determined by radioimmunoassay was mostly undetectable and did not respond to the administration of thyrotropin-releasing hormone (trh) determined by radioimmunoassay was mo ...	1976	177439
transcription-translation and translation-messenger rna decay coupling: separate mechanisms for different messengers.	antibiotics were used to inhibit protein synthesis at specific steps in the biosynthetic pathway. in this way, it was possible to study the coupling of protein synthesis to the accumulation of biologically active mrna in t4-infected escherichia coli. functional mrna for the phage enzymes deoxynucleotide kinase (ec 2.7.4.4; atp: nucleoside monophosphate phosphotransferase or nucleosidemonophosphate kinase) and alpha-glucosyltransferase (ec 2.4.1.5; 1, 4-alpha-d-glucan: 1, 6-alpha-d-glucan 6-alpha ...	1976	177977
adjuvant chemoimmunotherapy in advanced lesions of the head and neck.	growth of malignant tumors of the head and neck has been associated with progressive impairment of immunologic activity. successful control of disease has been shown to reverse this defect. preliminary results of a nonrandomized trial reduced recurrence of head and neck cancer for a median time of 11.5 months in eight of 10 patients who received chemoimmunotherapy adjuvant to surgical resection or radiation treatment. the number of patients in the series was small and the followup time too short ...	1976	178233
phosphorylation of double-stranded dnas by t4 polynucleotide kinase.	the phosphorylation by t4 polynucleotide kinase of various double-stranded dnas containing defined 5'-hydroxyl end group structures has been studied. particular emphasis was placed on finding conditions that allow complete phosphorylation. the dnas employed were homodeoxyoligonucleotides annealed on the corresponding homopolymers, dna duplexes corresponding to parts of the genes for alanine yeast trna, and a suppressor tyrosine trna from escherichia coli. the rate of phosphoylation of dnas with ...	1976	178357
attachment of gonococci to sperm. influence of physical and chemical factors.	neisseria gonorrhoeae of pilated t1 and nonpilated t4 colony types attached themselves to human sperm in greatest numbers and most reproducibly when suspensions of the cells were incubated at 35 degrees c in ringer's solution, ph 6-8. after incubation for 15 or 30 min. in a water bath shaker, 50 per cent. of human sperm had t1 gonococci attached and 25 per cent. t4. sperm and both types of gonococci were pre-incubated separately with various chemical agents, selected because the agent is found i ...	1976	178405
the acute thyroidal response to iodixed oil in severe endemic goiter.	the acute changes in serum thyrotropin (tsh) triiodothyronine (t3) and thyroxine (t4), were measured in 14 subjects, 4 to 10 (mean = 6) days following intramuscular iodized oil injection. in 8 subjects with small or absent goiter there was a significant fall in t3 and a rise in tsh concentrations, suggesting an acute inhibitory effect of the iodine. in 6 subjects with large multinodular goiters there was a marked rise in both t3 and t4, and reduction in the elevated basal tsh. biochemical hypert ...	1976	178686
surgical treatment of advanced carcinoma of the floor of the mouth.	the anterior arch of the mandible is essential for satisfactory oral function and appearance. it can be preserved in surgical treatment of advanced cancers of the floor of the mouth by partial thickness resection. surgery and radiation are approximately equally effective in the control of advanced (t3) primary lesions. a treatment plan is proposed which employs a combination of nondisabling surgical resection followed by immediate postoperative radiation. of 19 patients with t2, t3, and t4 lesio ...	1976	179393
joining of simian virus 40 dna molecules at endonuclease r eco ri sites by polynucleotide ligase and analysis of the products by agarose gel electrophoresis.	dna molecules cut with endonuclease r eco ri can be joined at eco ri cleavage sites by incubation with polynucleotide ligase. in order to define the optimum conditions for this reaction, linear simian virus 40 dna molecules (sv40(lri)) produced by endonuclease r eco ri cleavage of sv40 form i dna were joined using polynucleotide ligases specified by bacteriophage t4 and escherichia coli. we have determined that the concentration of the substrate dna molecules is the most important factor determi ...	1976	181070
comparison of prostaglandin e1 and tsh stimulation of cyclic amp synthesis in thyroid tissues from euthyroid subjects and thyrotoxic patients.	possible differences of the mode of action of tsh and prostaglandin e1 (pge) on the synthesis of cyclic amp were studied in normal human thyroids (normal thyroid) and thyroids from thyrotoxic patients (toxic thyroid). tsh was less effective in toxic thyroids than in normal thyroids; whereas pge1 was equally effective in normal thyroids and toxic thyroids. since the basal level of cyclic amp was the same in normal and toxic thyroids, this lower sensitivity of toxic thyroids to tsh was not due to ...	1976	181393
synthesis of new cyclic nucleotides and their differential stimulatory effects on thyroid function in mice.	various 8-substituted camp analogues were converted into the corresponding n6,o2'-dibutyryl, n6-monobutyryl, or o2'-monobutyryl derivatives and the parent compounds as well as the new derivatives were tested for tsh-like stimulation of the thyroid function in mice in vivo by means of the mckenzie bioassay. it was found that a pronounced stimulatory effect on the thyroid secretion can be produced by certain cyclic nucleotides. the most potent compounds, 8h2n-camp, 8mes-camp, and 8n3-camp at 15-30 ...	1976	181577
[plasma acth, sth and other hormone levels in various groups under chlormethiazole, haloperidol or reserpine load in alchohol delirium, alcoholic hallucinations, and chronic alcoholism].	studies of 135 men with safely diagnosed alcohol delirium mostly revealed increased acth blood values when sober and increased t4 values in about 1/3 of these patients. there is a correlation between the psychiatric clinical picture of the alcohol delirium and the acth content of the plasma. under load with chloromethiazole, halperidole or with reserpine, there is a significant drop in the increased acth and t4 values. in an acute alcoholic hallucinosis (n=16) similar endocrinological changes as ...	1976	181772
sexual impotence: studies of the hypothalamic-pituitary-thyroid axis and the effect of oral thyrotropin-releasing factor.	thyrotropin-releasing factor was given orally to 12 impotent patients in a dose of 43 mg daily for 4 weeks in a double-blind crossover technique and compared with placebo. trf showed no beneficial effect over placebo. in these patients, thyroid function and the hypothalamic-pituitary-thyroid axis were examined by measuring t3, t4, and tsh before and after the trf test. there were no pathological findings for patients included in this study.	1976	182105
further purification and characterization of t4 endonuclease v.	t4 endonuclease v, which is involved in repair of ultraviolet-damaged dna, has been purified 3600 fold from t4d-infected escherichia coli. the enzyme shows optimal activity at ph 7.2 and does not require added divalent ions. endonuclease v attacks both native and heat-denatured dna provided that the dna has been irradiated, and the enzyme activity is dependent on the dose of ultraviolet irradiation. the rate and the extent of the reaction are greater with irradiated native dna although the km va ...	1976	182258
repair of dna damaged by methyl methanesulfonate in bacteriophage t4.	a highly purified preparation of t4 endonuclease v does not degrade dna alkylated with methyl methanesulfonate, and the methyl methanesulfonate sensitivity of t4 wild type and x mutant is not affected by the v mutation. thus, t4 endonuclease v, the v gene product, does not seem to be involved in a repair or an abortive repair of methyl methanesulfonate-damaged t4 dna. the x and y genes of t4 and the pola and the uvrd genes of escherichia coli are concerned with the repair of methyl methanesulfon ...	1976	182259
interaction between thyroid hormones and erythrocyte membranes: competitive inhibition of binding 131 i-l-triiodothyronine and 131 i-l-thyroxine by their analogs.	molecular structural characteristics of thyroid hormones which influence binding to the erythrocyte membranes were investigated by competitive binding experiments. the ability of thyroid hormone analogs to displace 131 i-l-thyroxine and 131 i-l-triiodothyronine from the membranes was considered evidence of their competitive binding. the diphenyl ether linkage (thyronine) was essential as compounds with a single aromatic ring were weakly competitive. the presence of three iodine atoms at 3, 5 and ...	1976	182449
effects of excess iodide and other anions on thyroid hormone secretion in normal or hypophysectomized rats treated with graded doses of thyroid hormone.	in order to obtain further information about the stimulatory action of excess iodide on thyroid hormone secretion in thyroxine (t4)-treated rats, experiments were performed in hypophysectomized rats, or rats treated with graded doses of t4 or triiodothyronine (t3).t3 as well as t4 played a permissive role in the production of the iodide effect in normal animals, but t3 was more effective than t4. excess iodide stimulated thyroid hormone secretion in hypophysectomized animals, this finding being ...	1976	182459
genetic transformation as a tool for detection of neisseria gonorrhoeae.	a rapid method for the detection of neisseria gonorrhoeae, making use of the ability of deoxyribonucleic acid samples from clinically isolated strains of this organism to transform nutritional mutants of a particular strain of n. gonorrhoeae, has been described. in addition to using isolated cultures, transforming deoxyribonucleic acid can be obtained directly from the material that adheres to swabs of the cervix or the urethra. the time interval for transfer of swabs to the diagnostic laborator ...	1976	182717
direct stimulation of bone resorption by thyroid hormones.	although hypercalcemia, osteoporosis, and increased bone turnover are associated with thyrotoxicosis, no direct effects of thyroid hormones on bone metabolism have been reported previously in organ culture. we have now demonstrated that prolonged treatment with thyroxine (t4) or triiodothyronine (t3) can directly increase bone resorption in cultured fetal rat long bones as measured by the release of previously incorporated 45ca. t4 and t3 at 1 mum to 10 nm increased 45ca release by 10-60% of tot ...	1976	182721
inactivation and mutation of coliphage t4 by aliphatic nitrosamides and methanesulphonates: in vitro recovery of infectivity of t4 inactivated by isopropyl methanesulphonate.	the inactivation and mutation (to r phenotype) of extracellular coliphage t4 wild-type by the monofunctional alkylating agents n-methyl- and n-ethyl-n-nitrosourea and isopropyl methanesulphonate were investigated. the rate and extent of change in phage infectivity observed during the post-treatment period were found to correlate with what is known of the mechanisms by which these agents react in vitro. loss of phage infectivity was found to occur during the period following treatment with these ...	1976	183110
joining of ribooligonucleotides with t4 rna ligase and identification of the oligonucleotide-adenylate intermediate.	t4 rna ligase was found to join a-a-a-a-a-a and 32pu-u-u-u in the presence of atp as cofactor. in this reaction the pyrophosphate of pu-u-u-u and pa was isolated by chromatography on a rpc-5 column, besides the joined product and the starting materials. this pyrophosphate was shown to be an intermediate in the joining reaction because of the fact that coupling with a-a-a-a-a-a to give the decanucleotide could be performed in the absence of atp. the structure of the oligonucleotide-adenylate was ...	1976	183186
pituitary-thyroid interrelationship in rats exposed to different environmental temperatures.	the present work intended to show interrelationships between triiodothyronine (t3), thyroxine (t4), thyroid stimulating hormone (tsh) and cyclic adenosine 3'5'-monophosphate (cyclic amp) in both the pituitary and the thyroid. experiments were carried out on animals exposed to 0 and 37 degrees c for 4 days and to 34 degrees c for 21 days. control animals were maintained at 22 degrees c. t4 serum levels of 2.6, 4.0 1.1 and 0.42 mug/100 ml serum, and t3 levels of 95, 135, 65 and 36 ng/100 ml serum ...	1976	183444
radiographic evaluation of endotracheal tube position.	a malpositioned endotracheal tube is a potential hazard to the intubated patient. ideally, the tube tip should be 5+/-2 cm from the carina when the head and neck are in neutral position. in 92 of 100 patients studied, the carina overlay t5, t6, or t7 on portable radiographs. therefore, even when the carina is not visible, it can be assumed that a tube tip positioned at the level of t3 or t4 is safe. the degree of neck flexion or extension at the time of radiography may be determined by evaluatin ...	1976	183529
the destiny of brachytherapy in oncology.	at the invitation of the american radium society, pierquin reviews his very large experience with continuous low dose rate irradiation of cancer. the target volume is treated with 6,000-7,000 rad in 3-10 days at a dose rate of 30-100 rad/hr. preference for continuous low dose rate irradiation is based on the earlier work of jean pierquin, georges richard, claudius regaud, and ralston paterson with radium sources. afterloading techniques with iridium-192 have been substituted allowing safer and m ...	1976	183539
[restoration of ultraviolet-induced unsceduled dna synthesis of xeroderma pigmentosum cells by insertion of t4 endonuclease v utilizing hvj (author's transl)].		1976	184492
action of ki, thyroxine and cyclic amp on [3h]uridine incorporation into the rna of thyroid slices.	potassium iodide (ki) has been shown to impair thyroid protein biosynthesis both in vivo and in vitro. the present study was performed in order to clarify its mechanism of action. ribonucleic acid (rna) synthesis was studied in beef thyroid slices with either [32p] or [3h]-uridine as labelled precursors. both ki and thyroxine (t4) at 10(-5) m significantly decreased rna labelling under our conditions. in other experiments rna degradation was examined in pulse-labelled and actinomycin d-treated s ...	1976	184634
[cytochemical aspects of human polynuclear neutrophils under the in vitro and in vivo action of trh].	a trh fixation in vitro occurs at the level of the human polynuclear neutrophil, which suggests putative membrane receptor (s) for the hypothalamic hormone. after trh administration, enzyme modifications (myeloperoxydase, alkaline phosphatase) and metabolism changes (pas, sudan black) happen in vivo within the neutrophil, showing a functional activation of that blood cell. two processes may be considered to explain those data: 1) the trh is directly fixed, as observed in vitro; 2) a mediate horm ...	1976	184893
[recent developments in the etiology and diagnosis of basedow's disease].	the etiology and diagnosis of grave's disease are briefly reviewed with particular reference to newer discoveries in the wide range of immunoglobulins which might act as humoral stimulators of the thyroid. diagnosis of hyperthyroidism has been greatly facilitated by radioimmunologic determination of serum t4 and t3 and indirect estimation of t4 (t4 index). in this way 85-90% of cases can be diagnosed with a single blood sample. trh tests and t3 suppression tests are only rarely required.	1976	186867
partial characterization of an endonuclease activity which appears in nuclease free t4 polynucleotide kinase.	a nuclease activity has been found to appear in preparations of t4 induced polynucleotide kinase which had originally been nuclease free. the nuclease introduced random nicks into t7 dna suggesting that it was an endonuclease. destabilization of the kinase molecule by osmotic shock or by the removal of reducing agents, atp or salts was shown to stimulate the endonuclease appearance. the molecular weight was found to be 32,000 +/- 10% by gel filtration on g100 sephadex. the nuclease was active ov ...	1976	188016
[dissociation between the effects of tsh and dibutyryl camp on thyroxine secretion by isolated thyroid cells. similarity of actions on iodide uptake and thyroxine synthesis (author's transl)].	we have compared the effects of tsh and dibutyryl-camp (dbc) on three parameters of iodine metabolism in isolated hog thyroid cells: iodide uptake, thyroxine (t4) synthesis and t4 secretion. as wilson, we observed a similarity between tsh (60 mu/ml) and dbc (3 mm) actions on iodide uptake and t4 synthesis. but dbc did not reproduce the tsh stimulation of t4 secretion. since tsh increased t4 secretion in the presence of dbc, the lack of effect of dbc was not due to an alteration of the secretory ...	1976	188377
bacteriophage t4 head morphogenesis. vii. terminal stages of head maturation.	several aspects of the terminal stages of t4 head maturation were investigated using ts and am mutants blocked at single steps of the assembly pathway. we had previously found that cells infected with mutants of gene 13, e.g., tsn38 and ame609, accumulated both stable (10 to 20%)- and fragile (80%)-filled head precursors (hamilton and luftig, 1972). here we showed the following for such gene 13-defective, mutant-infected cells. (i) using thin-section analysis the pool of phage precursor structur ...	1976	130500
postinfection control by bacteriophage t4 of escherichia coli recbc nuclease activity.	infection by bacteriophage t4 has previously been shown to cause a rapid inhibition of the host recbc dnase, an atp-dependent dnase that is required for genetic recombination in escherichia coli. we report here the partial purification of a protein ("t4 rec inhibitor") from extracts of t4-infected cells and some characteristics of the in vitro inhibition reaction with purified inhibitor and recbc nuclease. this inhibitory activity could not be purified from extracts of uninfected e. coli. both t ...	1976	130501
alteration in intracellular sodium concentration and ouabain-sensitive atpase in erythrocytes from hyperthyroid patients.	the activity of the ouabain-sensitive sodium-potassium-activated component of the atp-hydrolyzing enzyme system (ouabain sensitive atpase) was studied in the erythrocyte membranes of 10 patients with hyperthyroidism, and found to be decreased in all 10 patients. the mean ouabain-sensitive atpase activity was 43 +/- 4 nmol pi/mg tissue/h in the patients, compared with 69 +/- 5 nmol pi/mg tissue/h in the erythrocyte membranes of 10 paired control subjects. the mean concentration of sodium within t ...	1976	132454
[preservation of a vertical mucosa strip for one stage gullet reconstruction after radical resection of the pharynx and larynx (author's transl)].	17 out of 22 patients with a squamous cell cancer of the hypopharynx (t2-t4,n1-n3) are treated by resection of the pharynx and larynx, combined with radical neck-dissection on both sides, total strumectomy and full course of radio-therapy, reaching from the base of the skull down to the madiastinum. for the immediate reconstruction of a nutritional pathway the preservation of a vertical tumor free strip of the pharyngeal wall at least 15 mm broad is recommended. the remaining mucosa resected at ...	1976	134208
[biosynthesis of thyroglobulin in an adult lamprey, lampetra planeri (bloch)].	thyroglobuline (tg) biosynthesis has been studied in an adult fresh water lamprey lampetra planeri (bloch), by injection in coelomic cavity or by in vitro incorporation in follicles of branchial region of various radioactive labels: 125i-, 3h-leucine, 3h-mannose, 3h-galactose, n-acetyl mannosamine and 3h-n-acetylglucosamine. labelling by all theses substances, present in tg of mammals, proceeds efficiently in two hours. it has been demonstrated that the cyclostome studied synthetizes a 18 s iodi ...	1976	134785
control of synthesis of mrna's for t4 bacteriophage-specific dihydrofolate reductase and deoxycytidylate hydroxymethylase.	a 30 degrees c, functional messengers for dcmp hydroxymethylase first appeared 3 to 6 min postinfection and reached their maximum levels at 12 min. chloramphenicol, added before the phage, reduced the rate of mrna accumulation. when the antibiotic was added 6 min postinfection, mrna levels increased at their normal rate but there was no obvious repression of messenger accumulation. delaying the addition of drug until 8 or 12 min had progressively less effect on the pattern of hydroxymethylase mr ...	1976	135096
evidence that mismatched bases in heteroduplex t4 bacteriophage are recognized in vivo.	t4 heteroduplex heterozygotes are lost selectively after prolonged incubation of phage-infected escherichia coli cells under nonreplicating conditions. the loss of heterozygosity occurs for four out of six rii sites tested and is not dependent upon t4 v gene function. the results are interpreted to indicate the existence of a base-specific system for the recognition of mismatched bases in intracellular dna.	1976	135847
incorporation of t4 phage dna into a specific dna fraction from the higher plant matthiola incana.	isolated t4 phage dna (sigma=1.694 g/ml) is applied to seedlings of the crucifer matthiola incana (dna density sigma=1.698 g/ml). the phage dna can partly be reextracted from the plants in a specific dna fraction, which is predominantly characterized by its unusual high density (high density complex=hdc; sigma=1.724 g/ml). dna:dna hybridization studies show that phage specific dna sequences are preserved in the hdc. results of brdurd labeling of the plant dna before and during incubation with t4 ...	1976	136126
[the effect of various amines on trh contents in rat brain (author's transl)].	to study the effect of vatious amines on trh contents in rat brain, various amines or inhibitor of synthesis of amines were injected into rat through i.v. or i.p.. rats were decapitated and brain was frozen in dry ice and aceton. trh contents in hypothalamus(h), cerebrum(c) and cerebellum and brain stem (c and s) were measured by trh radioimmunoassay. trh contents in normal rats were 3.9+/-0.5ng in h, 2.6+/-0.5ng in c and 1.6+/-0.3ng in c and s. trh contents in all parts of brain were increased ...	1976	138603
[effect of thyroidectomy on circulating thyroid hormones in dogs administered insulin].	in the blood of intact dogs, insulin increases the labelled thyroid hormones (pbi) by about 30% and doubles the t3 level. in athyreotic insulin-administered dogs, the circulating t4 and t3 remain at more or less unvarying values, the plasma disappearance slope of radiothyroxine is not altered. such data suggest that without the thyroid gland, insulin has no action on the level of the blood thyroid hormones.	1976	67881
[a case report of hashimoto's disease with anti-thyroxine autoantibody (author's transl)].	in one case of untreated hashimoto's disease, serum thyroxine (t4) value by radioimmunoassay (ria) was significantly lower than that by competitive protein binding analysis (cpba). the discrepancy was found to be due to the presence of antithyroxine autoantibody in the serum. this phenomenon was considered to be of practical importance in interpreting the t4 value by ria in cases with autoimmune thyroid diseases. the patient was 59-year-old woman with a 30-year history of goiter. a diagnosis of ...	1976	65300
preparations and properties of ribonucleic acid polymerase from acinetobacter calcoaceticus.	deoxyribonucleic acid (dna)-dependent ribonucleic acid (rna) polymerase (ec 2.7.7.6) from acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the escherichia coli b enzyme. the molecular weights of the two native active enzymes as well as their alpha and beta subunits appeared to be similar. no subunit corresponding to that of sigma from e. coli was found, and furthermore no separation between the beta subunits could be detected by gel ...	1976	1380
reflex sympathetic tachycardia during intravenous infusions in chronic spinal cats.	the reflex tachycardia elicited by rapid intravenous infusions of a blood substitute was studied in 21 chronic cats with spinal sections at c8. all animals could breath spontaneously. the day after section the average resting heart rate (hr) and arterial pressure (ap) were 109 beats/min and 98/67 mmhg, respectively. vagal blockade with atropine (0.5-0.7 mg/kg iv) was performed prior to each infusion, increasing the average hr to 127 beats/min. in 39 infusions in 21 cats the average increase in h ...	1976	3115
isoaccepting species of serine trna coded by bacteriophage t5sto.	by aminoacyl-trna-dna hybridization and chromatographic analysis, evidence was provided that the bacteriophage t5sto codes for two trnaser species. trinucleotide- or polynucleotide-stimulated binding experiments assigned the codons ucc or ucu to these two trnaser species. they also suggested that the synthesis of these two trnaser species does not modify the reading capacity for codons less used in escherichia coli f and corresponds to a different situation compared with the t4-coded trna's.	1976	3665
biological properties of an improved transformation assay for native and denatured t4 dna.		1976	3880
ribonucleic acid synthesized in meiotic cells of saccharomyces cerevisiae: effect of culture medium ph.	pulse-labeled ribonucleic acid (rna) was extracted from polysomes of sporulating cells of saccharomyces cerevisiae and characterized in sucrose gradients and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. transfer rna, ribosomal rna, and heterodisperse rna, presumed to be messenger rna, were synthesized during a 20-min pulse at t4 and t6 when labeling was performed in sporulation medium adjusted to ph 6.0. furthermore, ribosomal rna was processed into functional ribosomes during t ...	1976	4430
5' leads to 3'-exonucleases of bacteriophage t4.	two enzyme activities which release nucleotides preferentially from the 5' termini of dna were found in t4-infected escherichia coli. since no corresponding activities were found in uninfected cells, the activities appeared to be induced by t4. both activities are capable of excising pyrimidine dimers from ultraviolet-irradiated dna which has been treated with t4 endonuclease v. one of the activities , referred to as t4 exonuclease b, was purified 400-fold from an extract of t4v 1- infected cell ...	1976	4453
demonstration and some properties of cytosol-binding proteins for thyroxine and triiodothyronine in human liver.	cytosol-binding proteins for l-thyroxine (t4) and triiodo-l-thyronine (t3) were studied in human liver specimens obtained at autopsy from 5 male and 2 female subjects. the liver cytosol containing 131i-t4 or t3, together with or without added stable hormones, was fractionated by pevikon thin-layer electrophoresis at ph 8.6, 8.0, and7.4. it was demonstrated in all the specimens that besides a small amount of serum t4-binding globulin, there existed three t4-binding proteins, termed ht4-1, ht4-2 a ...	1976	4465
heat mutagenesis in bacteriophage t4: the transition pathway.	g-c leads to a-t transitions are induced by heat, and arise from the deamination of cytosine (5-hydroxymethylcytosine in the case of bacteriophage t4) generating uracil. the reaction is proton-catalyzed, and is also characteristic of acid mutagenesis. mutation rates and activation energies of mutation are site-specific, and are presumably influenced by neighboring bases. rates of heat-induced mutation in bacteriophage t4 under conditions of temperature, ph, and ionic strength similar to those pr ...	1976	4797
bacteriophage t4 prehead proteinase. i. purification and properties of a bacteriophage enzyme which cleaves the capsid precursor proteins.		1976	12371
packaging of genomes in bacteriophages: a comparison of ssrna bacteriophages and dsdna bacteriophages.	in complex dna bacteriophages like lambda, t4, t7, p22, p2, the dna is packaged into a preformed precursor particle which sometimes has a smaller size and often a shape different from that of the phage head. this packaging mechanism is different from the one suggested for the rna phages, according to which rna nucleates the shell formation. the different mechanisms could be understood by comparing the genomes to be packaged: single stranded fii rna has a very compact structure with high helix co ...	1976	13425
studies on the maturation of the head of bacteriophage t4.	the presentation focuses on the structural rearrangements of the subunits and the processing of the various protein constituents which accompany the maturation events of the head of bacteriophage t4. the major features of the maturation steps of the head are the following: (a) the viral dna is pulled into an empty head in a series of events; (b) cleavage of two core proteins, p22 (mol. mass = 31000), to small fragments and the internal protein ipiii (mol. mass = 23000) to ipiii (mol. mass = 2100 ...	1976	13426
dna viruses: cooperativity and regulation through conformational changes as features of phage assembly.	the assembly of bacteriophages provides experimental model systems for the study of regulation at the level of gene products. we discuss the hypothesis of regulation through sequentially induced conformational changes by which precursor-assemblies become ready at a specific stage of maturation to interact with an additional gene product or nucleic acids. phage mutants provide excellent experimental model systems for studying, for example, the role and fate of the core in the prehead assembly. th ...	1976	13433
the thyroxine-binding properties of rat and rabbit serum proteins.	the thyroxine (t4) binding properties of rat and rabbit serum proteins were studied using a gel equilibration technique, gel filtration and polyacrylamide gel electrophoresis. in both species two different t4 binding molecules were identified in whole serum and in cohn fraction v preparations. only one of these binding species demonstrated the characteristics of specific binding, i.e., high affinity for the hormone and binding site saturability. the mean (+/- sd) apparent association constants ( ...	1976	55359
resin hemoperfusion: a method of removing circulating thyroid hormones.	the ability of an extracorporeal hemoperfusion system employing neutral amberlite resin to bind thyroid hormone and to decrease circulating levels of triiodothyronine (t3), thyroxine (t4), and free thyroxine (ft4) was evaluated in dogs made thyrotoxic by the intramuscular administration of thyroid hormone. since the resin column and tubing were charged with saline, the effects of hemodilution from this source on serum t3 and t4 was assessed by control perfusion through a column which did not con ...	1976	55416
[the effect of contrast media containing iodine on selected in vitro thyroid test procedures (author's transl)].	the effect of 6 commonly used contrast media on the results of 3 different methods for determination of thyroid hormone concentration in the serum is examined in vivo. t4 determination by column chromatography proved to be very liable to distortion for contrast media. with some contrast media the res-o-mat-etr test proved unreliable. t4 determination by means of protein binding analysis is not affected by contrast media in the direction of false raised values.	1976	55963
a transient rise of hormone secretion: a response of the stimulated rat thyroid gland to small increments of iodide supply.	small doses of iodide (2 times 3.2 mug at 12 h interval), below those capable of inducing wolff-chaikoff effect, were injected into rats kept on a moderately low iodine diet. by means of a 125i equilibration technique as well as by direct measurement of cold t4, it was demonstrated that the level of circulating pb125i (representing iodothyronines as confirmed by column chromatography) increased by a mean of 40% within 24 h following the first iodide injection. the serum tsh concentration (measur ...	1976	56115
thyrotoxicosis with painless thyroiditis.	four women and one man with painless subacute thyroiditis presented with hypermetabolic signs and symptoms. thyroxine (t4) and triiodothyronine (t3) resin uptakes (t3r) were increased but the 24 hour radioactive iodine (rai) uptakes were less than 1 per cent. surreptitious use of thyroid hormone was excluded. the thyroid was enlarged in one patient and nontender in all. exophthalmos was absent. the protein-bound iodine level was 1.1 to 9.5 mug/dl greater than the t4 level. the sedimentation rate ...	1976	56130
metabolism of thyroxine-binding globulin in man. abnormal rate of synthesis in inherited thyroxine-binding globulin deficiency and excess.	it has been previously suggested that inherited thyroxine-binding globulin (tbg) abnormalities in man may be due to mutations at a single x-chromosome-linked locus controlling tbg synthesis. however, abnormalities in tbg degradation have not been excluded. the availability of purified human tbg and its successful labeling with radioiodide allowed us to examine such possibility. human tbg was purified by affinity chromatography, labeled under sterile conditions with 131i or 125i,, and mixed with ...	1976	56342
isolation and characterization of t-even ghost-tolerant mutants of escherichia coli.	mutants of escherichia coli tolerant to the ghosts of t-even phages (t2, t4, and t6) have been isolated from a strain supersensitive to t6 phage. first, t6 supersensitive mutants were isolated from mutagenized e. coli w2252 by replica plating to t6 phage-overlaid agar. one of them, strain nm101, was mutagenized again, grown, and then plated with a high multiplicity of t4 and t6 ghosts. surviving cells were checked for tolerance to ghosts and adsorption of phages. one such ghost-tolerant mutant, ...	1976	58859
an improved quantitative plaque assay for lymphocytes bearing antigen-specific cell receptors.	an improved method for the determination of the number of lymphoid cells bearing antigen-specific receptors is described. the method is based on the use of hapten-coupled bacteriophage (dinitrophenyl-t4) and detection of lytic plaques formed by the action of dnp-t4 on a target e. coli strain. the method is highly specific (up to 90% specific binding) and can be adapted for use with other antigenic determinants chemically attached to an active bacteriophage.	1976	58942
hemodynamics of hyperthyroidism. the effects of autonomic nervous blocking and anti-thyroid drug treatment.	this work was intended to analyze the sympathetic and parasympathetic factors affecting the hemodynamics of hyperthyroidism. seven patients with hyperthyroidism, diagnosed based on the determinations of bmr, 131i-uptake, t3-resin sponge uptake (t3-rsu), and serum level of thyroxine (t4) were subjected to the study. the hemo-dynamic estimation was done (1) at rest, (2) after vagus blocking by the injection of 0.04 mg/kg b.wt. atropine and 0.2 mg/kg b.wt. propranolol before (hyperthyroid state=h) ...	1976	59000
effect of a single dose of dexamethasone on serum concentrations of thyroid hormones.	in ten euthyroid subjects, in whom endogenous thyroid-stimulating hormone (t.s.h.) production was suppressed by oral thyroxine (t4), a single dose of dexamethasone resulted in reduced serum-3,3'5-triidothyronine (t3) concentration and raised serum-3,3',5'-triiodothyronine (reverse t3 or rt3) concentration after 24 h. these changes were not related to changes in free hormones or binding proteins. adrenal glucocorticoids may have a pathophysiological role in modulating the peripheral metabolism of ...	1976	59147