Publications

long-term observation of thyroid function after surgical treatment of thyrotoxicosis.	during the 10-year period from 1960 to 1970 a total of 213 patients with diffuse or nodular toxic goitre underwent subtotal thyroidectomy. a short-term preoperative treatment with antithyroid drugs and iodine was given systematically. the observation period ranged from 5 to 15 years, average 9 years. a total of 203 patients (95%) were traced and followed up. the concentration of se-t4, se-t3, and se-tsh was measured and in selected cases a trh test and bmr was carried out in addition. all thyroi ...	1977	906756
induction of the thyroxine (t4) to triiodothyronine (t3) converting enzyme in rat liver by thyroid hormones and analogs.	in thyroidectomized, unsubstituted rats the t4 to t3 converting activity of liver homogenate is reduced to about 30% of that in unoperated control animals. the enzyme activity can be reinduced dose-dependently with t4. to achieve a normal activity, high, non-physiological plasma t4 concentrations are needed. plasma t3 levels are much better correlated to the t4 to t3 converting activity. pure t3 proved to be a more potent enzyme inducer than t4. no difference could be detected between l- and d-t ...	1977	908148
the behaviour of thyroid hormones in an infant with untreated neonatal thyrotoxicosis.	the clinical course from birth and serial measurements of serum t3, t4 and tsh in an infant with untreated neonatal thyrotoxicosis are reported. the thyroid hormone levels fell exponentially with time at rates very much slower than those previously reported for the maternally-transmitted thyroid stimulating antibody generally thought to cause the disorder. steady physiological levels of thyroid hormones were achieved after 110 days (serum t3 = 3.4 nmol/l, t4 = 118 nmol/l). tsh first rose to a me ...	1977	908154
a simple synthesis of [3,5-125i]diiodo-l-thyroxine of high specific activity.	the customary methods for labeling t4 and its analogs, iodination or exchange-labeling, are not applicable to those iodothyronines whose iodine atoms are exclusively in the nonphenolic ring. therefore, [3,5-125i]diiodo-l-thyronine had to be synthesized by a different metnod. this synthesis involves the coupling of [125i]diiodo-l-tyrosine with 4-hydroxy-3,5-diiodophenylpyruvic acid to form l-thyroxine labeled in the nonphenolic ring, followed by removal of the two unlabeled iodine atoms in the ph ...	1977	908276
extra-long bacteriophage t4 tails produced under in vitro conditions.		1977	909089
triiodothyronine and thyroxine content of desiccated thyroid tablets.	triiodothyronine (t3) and thyroxine (t4) were measured by radioimmunoassay in pronase hydrolysates of four lots each of 1- and 2-grain tablets of desiccated thyroid (thyroid, armour) and thyroglobulin (proloid, warner-chilcott). the methodology used was verified by studies of tablets containing known quantities of t4 and t3. one grain of desiccated thyroid contained 12 +/- 1 and 64 +/- 3 microgram (mean +/- sd) of t3 and t4 per tablet, respectively (t4/t3 molar ratio, 4.3). a 1-grain tablet of t ...	1977	909397
the nucleotide sequence of two restriction fragments located in the gene ab region of bacteriophage s13.	the nucleotide sequence of a double stranded dna fragment from the gene ab region of bacteriophage s13 dna has been determined. the fragment was isolated as two adjacent shorter fragments by cleavage of s13 replicative form (rf) dna with restriction endonuclease iii from hemophilus aegyptius. the strands of the fragments were separated electrophoretically and hydrolyzed with t4 endonuclease iv to yield short oligonucleotides which were then sequenced by partial exonuclease digestion. the complet ...	1977	909772
nucleotide sequence of 3' untranslated portion of human alpha globin mrna.	we have determined the nucleotide sequence of 75 nucleotides of the 3'-untranslated portion of normal human alpha globin mrna which corresponds to the elongated amino acid sequence of the chain termination mutant hb constant spring. this was accomplished by sequence analysis of cdna fragments obtained by restriction endonuclease or t4 endonuclease iv cleavage of human globin cdna synthesized from globin mrna by use of viral reverse transcriptase. analysis of crna synthesized from cdna by use of ...	1977	909779
stability of phage t4 lysozymes. i. native properties and thermal stability of wild type and two mutant lysozymes.	two mutants of phage t4 lysozyme were prepared and characterized. one mutation substituted a tyrosine residue for tryptophan at position 138. the other substituted tyrosines at all three tryptophan positions of the wild type molecule (126, 138, 158). comparative studies of the physical properties (absorption, fluorescence, circular dichroism) of the three enzymes were performed as a function of ph. also, the proteins were reversibly melted as a function of ph. since the unfolding reaction appear ...	1977	911878
molecular arrangement of dna in bacteriophage t4.		1977	912002
[double antibody radioimmunoassay of t4 and t3 using "eiken" kit].		1977	912936
["hypothyroid" goitre in puberty treated with a combined t3/t4 preparation (author's transl)].	after administration of combined t3/t4 preparations (ratio of 4 : 1) to juveniles with goitre, size ii-iii (who), and hypothyroid t4 or pbi levels, the t3 value reached hyperthyroid levels 7-8 hours afterwards. the increased t3 level with normal t4 blood levels can lead to iatrogenic t3 hyperthyroidism. serial tests demonstrated that at increasing doses a raised t3/t4 ratio, which is observed with low t4 values, can return to normal but with increasing doses this ratio, caused by t3 rise, can ag ...	1977	913268
[quantitative aspects of the formation of a half chromatid chiasma as an intermediate product of genetic recombination in bacteriophages].	formal genetic consequences of generation and break-up of a half chromatid chiasma have been studied on the basis of a model with the symmetric exchange of two strands between parental dna molecules. in recombination analysis, generation and break-up of this structure gives rise to a critical frequency r (zeta), which corresponds to recombination between two markers separated by a distance equal to the mean genetic length zeta of the hybrid region at the time of scission of the cross connection. ...	1977	914034
u-g-a suppressor of bacteriophage t4 associated with arginine transfer rna.	a u-g-a suppressor of bacteriophage t4, designated psu4+op, has been isolated and characterized. the transfer rna species previously shown to have an anticodon sequence complementary to arginine codons is affected by the psu4+op mutation. wild type and psu4+op arginine trnas have the same sequence except for their anticodons, where u-c-u in the wild type species is mutated to u-c-a in the psu4+op species. this mutation is believed to confer u-g-a suppressor activity on the psu4+op arginine trna.	1977	914869
ultracentrifugal characterization of cytosol-binding proteins for thyroxine and triiodothyronine in human liver.	sucrose density gradient ultracentrifugation was performed on human liver cytosol containing double tracers of 131i-thyroxine(t4) and 125i-triiodothyronine(t3) together with or without large amount of stable hormones. tracer t4 bound to the cytosol indicated 2 peaks of 8.3s and 4.7s, both of which were displaced with stable t4, but not with t3. maximal binding capacity of the 8.3s component averaged 43.2 microgram/100ml of 33% cytosol. tracer t3 in the cytosol showed a single peak of 4.2s which ...	1977	914987
particle counting immunoassay (pacia). i. a general method for the determination of antibodies, antigens, and haptens.	by using a device designed for counting blood cells, it is possible to measure the agglutination of polystyrene beads (0.8 mu) with accuracy and great sensitivity, the agglutination resulting in a reduction in the number of particles. the latter coated with antigen can be used for determining igm or igg antibodies e.g. human rheumatoid factor or rabbit anti-bovine serum albumin antibodies. macromolecules with multiple antigenic determinants agglutinate particles carrying specific antibodies. thi ...	1977	915310
distinctive protein requirements of replication-dependent and -uncoupled bacteriophage t4 late gene expression.	this paper further explores the relationship of viral dna replication to bacteriophage t4 late gene expression. it is shown that replication coupled and -independent late transcription make different qualitative or quantitative demands on phage protein synthesis. in further analysis of these different protein synthesis requirements, experiments were performed with a temperature-sensitive mutant in t4 gene 55 (ts553). it is known that the gene 55 product regulates t4 late gene expression and bind ...	1977	916023
isolation and mapping of t gene mutants of bacteriophage t4d.	a procedure for selective isolation of t4 t mutants is described. at 120 min after infection of escherichia coli cells with a low multiplicity of t4 bacteriophage, the mixture was sedimented through a linear sucrose gradient, and infected cells that remained intact were collected as the fastest sedimenting fraction. ten to 50% of the phage released by chloroform treatment of this fraction were t mutants. collection of a high proportion of t mutants depended on efficient elimination of cells that ...	1977	916026
thyroid function in children with chronic renal failure.	thyroid function was evaluated in 24 children (aged 4-18 years) with chronic renal failure either before institution of hemodialysis or after more than 3 months of hemodialysis. 22 patients were clinically euthyroid and 2 were hypothyroid; in one case hypothyroidism was secondary to cystinosis and in the other it followed radiation therapy. the 2 hypothyroid patients had subnormal levels of t4, t3, fti and ft4 as well as elevated serum tsh levels. mean values for t4, t3, fti and ft4 for the rema ...	1977	917171
[screening for hypothyroidism in the newborn with a total t 4-ria method (author's transl)].	a screening method for detection of congenital hypothyroidism is presented in detail in cooperation with the "austrian program for inborn errors of metabolism". screening is performed by measuring the total t4-content of 1/8 inch filter paper dots filled with dried blood of newborns. the strategy for recall of newborns with borderline or pathological t4-values used, results in a definite diagnosis on day 55 of life. the advantages of additional tsh determination in the filter paper dots are disc ...	1977	917574
studies on replacement and suppressive dosages of 1-thyroxine.	serum thyrotropin (tsh) levels were studied in 55 hypothyroid patients in order to determine adequate replacement and suppression dosages of 1-thyroxine (t4). in accordance with previous reports it was found that most patients had normal tsh levels and were clinically euthyroid at daily doses of 0.10-0.15 mg t4. none of the patients required a dose exceeding 0.20 mg. when the tsh levels normalized, serum thyroxine and serum triiodothyronine also fell to levels within their normal ranges. the eff ...	1977	920242
[serum thyroxine determination: comparison and clinical significance of radioimmunoassay and competitive binding assay (t4-ria pack and thyopac-4)].		1977	923114
effects of diuretics on thyroid function of guinea pigs.	uptake of radioiodide by the isolated thyroid and serum concentrations of thyroxine (t4) and tri-iodothyronine (t3) were determined in guinea pigs pretreated with ethacrynic acid (20 mg/kg), furosemide (40 mg/kg) or hydrochlorothiazide (40 mg/kg). it was found that both ethacrynic acid and furosemide suppressed the 131i uptake by the isolated thyroid tissues. in addition, thyroid weight and serum t3 concentration were lower in ethacrynic acid-treated animals. it seems that some diuretics, partic ...	1977	923646
triiodothyronine-producing metastatic follicular thyroid carcinoma.	thyroid hormone serum concentration was determined in a thyroidectomized patient with functional follicular thyroid carcinoma with metastases. marked diminution of circulating serum t4 and ft4 with normal t3 serum concentration was found. the clinical implications of this shift in secretory activity are discussed.	1977	924359
priming of deoxyribonucleic acid synthesis on phage fd and phix174 templates by oligoribonucleotides contaminating nucleoside 5'-triphosphates.	commercial preparations of guanosine 5'-triphosphate and deoxyguanosine 5'-triphosphate are contaminated with oligoribonucleotides 4 to 6 residues in length. the oligoribonucleotides can be separated from the nucleoside 5'-triphosphates by chromatography on deae-sephadex a-25 or by gel filtration through sephadex g-25. the oligoribonucleotides are effective primers for the dna polymerase of bacteriophage t7 on the single-stranded circular dnas of phage fd and phix174; they are covalently attache ...	1977	925008
role of deoxycytidylate deaminase in deoxyribonucleotide synthesis in bacteriophage t4 dna replication.		1977	925015
serum thyroxine concentrations in larval and metamorphosing anadromous sea lamprey, petromyzon marinus l.	thyroxine (t4) concentrations in samples of fresh sera taken from fed and unfed larval (ammocoetes) and metamorphosing anadromous sea lamprey (petromyzon marinus) were measured by means of radioimmunoassay. t4 levels in unfed ammocoetes after a 1-month period were not significantly different from those of the fed ammocoetes. nutritional state of ammocoetes therefore does not seem to affect their serum t4 levels. serum t4 concentrations decreased abruptly at the onset of metamorphosis and decline ...	1977	925662
upper alveolar carcinoma--a 30 year survey.	primary malignancy of the upper alveolus is an infrequent type of intra-oral carcinoma. this study reviews in detail 50 patients with a primary tumor of the upper alveolus who were treated at the cancer control agency of british columbia and vancouver area hospitals, between 1940-1970 inclusive. sixteen per cent had exposure to possible industrial carcinogens; 34 per cent had previous leukoplakia. the common presenting symptoms were pain (78 per cent) and swelling (64 per cent). only 22 per cent ...	1977	926221
genetic identification of cloned fragments of bacteriophage t4 dna and complementation by some clones containing early t4 genes.	bacteriophage t4 dna containing cytosine has been obtained from cells infected with phage mutant in genes 42, 56, dena and denb. this dna can be cut by a number of restriction endonucleases. fragments obtained by digestion of this dna with ecori have been cloned using the vector plasmid pcr1. clones containing t4 dna were identified by hybridization with radioactive early and late t4 rna. a simple marker rescue technique is described for the genetic identification of the cloned t4 fragments. som ...	1977	927440
[thyrotropic activity of the pituitary gland in rats at various age periods].	the thyrotropic activity of the hypophysis (tth synthesis and secretion) was studied in rats at various age periods. the work was conducted on mongrel male rats of four age groups: 1 1/2-2, 8-10, 18-24, and 28-32-months old. being relatively low in the sexually immature animals, the thyrotropic activity of the hypophysis considerably intensified in adult rats and diminished with the ageing of the organism. a reduction of the tth synthesis and secretion in old animals, testified to by the hormone ...	1977	928330
[standardization of a new thyroid gland suppression test by means of 3000 microgram l-thyroxine].	the suppressibility of the tsh-thyroid axis one week after a single dose of 3000 microgram l-thyroxine was studied in 21 euthyroid subjects. in all cases the normal thyroid function was proven by euthyroid values for thyroxine (t4), free thyroxine index (ft4-i), triiodothyronine (t3), radioiodine uptake, pbi-131, a normal scintiscan and by a physiologic tsh response to thyrotropin-releasing hormone (trh). compared to the baseline values the mean suppression of radioiodine uptake at 2,24 and 48 h ...	1977	929149
[primary coblat therapy and secondary surgery for supra-glottal carcinoma (author's transl)].	this statistical study concerned 218 carcinomas of the laryngeal vestibule and margin treated at the curie foundation between 1958 and 1967, by primary cobalt therapy followed when necessary by secondary total laryngectomy. it shows that cobalt therapy alone gives excellent results in early forms (t1 and t2) even in the presence of n1 or n2 nodes. by contrast, the proportion of cures is much lower in t3 and t4 forms. secondary total laryngectomy was used in only 25 cases out of 127 failures. the ...	1977	931295
evidence for dopaminergic control of thyrotrophin secretion in man.	after administration of the dopamine-receptor-blocking drug, metoclopramide (10 mg orally), there is significant release of thyrotrophin (t.s.h.) in hypothyroid patients which is not evident in euthyroid subjects. this is not due to spontaneous fluctuation in basal t.s.h. levels, and it indicates inhibitory dopaminergic control of t.s.h. release in man. the lack of significant t.s.h. release in euthyroid subjects may be due to the inhibitory effects of normal circulating levels of t3 and t4 on t ...	1977	70641
the clinical value of serum triiodothyronine, thyroxine and thyrotropin estimations during medical antithyroid treatment.	the relation between clinical status and serial determinations of serum t3, serum t4 and serum tsh has been evaluated in the early phase of medical antithyroid treatment in 12 unselected hyperthyroid patients, and in 19 patients who later during treatment accidentally developed low serum t4 values. determination of both serum t3 and serum t4 was found necessary to avoid undertreatment. two patients with signs of hypothyroidism in the early phase developed low serum t4, while serum t3 and serum t ...	1977	70965
[changes in serum t3, t4 and tsh before and after the t3-suppression test in patients treated for graves' disease (author's transl)].		1977	72007
blood levels of cyclic amp, thyroxine, uric acid, certain metabolites and electrolytes under heat-stress and dehydration in the pigeon.	the effect of heat stress and dehydration on certain blood parameters were studied in the pigeon. pigeons exposed to heat-stress and dehydration showed higher haematocrit values. serum cyclic amp levels, both with and without corrections made for difference in haematocrit, showed a more than two-fold increase in heat-stressed and dehydrated pigeons. serum triglyceride level (before and after correction for haematocrit difference) in the experimental birds dropped to less than half that in the co ...	1977	72533
protamine messenger rna from rainbow trout testis contains the nucleotide sequence a-a-u-a-a-a in an untranslated region.	full-length, complementary dnas were prepared to rainbow trout protamine mrna using reverse transcriptase and were labelled during synthesis by the replacement of datp by [alpha32p]datp or dttp by [alpha32p]dttp. the 32p-labelled protamine complementary dnas were digested with t4 endonuclease iv. fragments from the digests were separated in two dimensions, and those discrete fragments which could be identified from both the a-labelled and t-labelled complementary dnas were subjected to sequence ...	1977	72566
serum thyrotropin response to thyrotropin-releasing hormone and free thyroid hormone indices in patients with familial thyroxine-binding globulin deficiency.	the response in serum thyrotropin (tsh) to synthetic thyrotropin-releasing hormone (trh) as well as serum free thyroxine index (ft4i) and free triiodothyronine index (ft3i) was investigated in six patients with familial thyroxine-binding-globulin (tbg) deficiency. the total serum thyroxine (t4) and triiodothyronine (t3) concentrations were significantly decreased, compared with those of normal subjects (3.4 +/- 0.9 microgram/dl, mean +/- sd. vs. 9.0 +/- 1.5 microgram/dl, p less than 0.01 and 87 ...	1977	72658
effects of total fasting in obese women. i. response of the thyroid.	the values of serum t4, t3, t4/t3, rt3u, ft4i, pbi, atr, tsh, 132i uptake and antibodies against thyreoglobulin were estimated in non-obese, healthy women and in obese women with a stabile body weight on total fasting of 14 days. obesity with no dietary restriction compared with normal controls was accompanied by a tendency to higher serum levels of t4 and t3 and prolonged atr. other indicators of thyroid function remained unchanged. total fasting led to a decrease of serum t3 and to an increase ...	1977	72659
study of the pituitary-thyroid functions at high altitude in man.	the alterations in serum levels of t3, t4, tsh and tbg, tsh response to 100 mug iv trh, and urinary excretion of t3 and t4 were studied in 8 healthy men at sea level (sl), on days 1, 2, 4, 8 and 16 after arrival by air at high altitude (3,700 m, ha), and during days 5 to 7 after their return to sl. no significant alterations in serum levels of tsh and tbg or tsh response to trh were observed during exposure to ha or on return to sl. there was, however, an acute elevation in both serum total t3 a ...	1977	65358
short and long term effects of radioiodine and antithyroid drugs on t4 binding proteins, free t4 and t3, during graves' disease therapy.	ninety five patients with graves' disease were studied before and at three months intervals after antithyroid drugs (atd) (31 cases) or radioiodine (64 cases) therapy until recovery. before treatment, the t4 maxima binding capacity of tbpa was significantly decreased 253.5 +/- 11.4 mug/100 ml)(mean + se) (control values: 287 +/- 10.4 mug/100 ml) (alpha = 0.04), especially in 53.7% of patients (m = 177 +/- 8 mug/100 ml). the mean of tbg (m = 20.7 +/- 0.9 mug/100 ml) was not different from euthyro ...	1977	66177
t4 radioimmunoassay and thyroxine-binding globulin concentration.		1977	66554
post-partum transient thyrotoxicosis with painless thyroiditis.	5 patients presented with transient thyrotoxicosis and painless thyroiditis in the post-partum period. thyrotoxicosis developed within 1-6 months of delivery. all had small non-tender goitres. initially, all patients had elevated values for thyroxine (t4), triiodothyronine (t3) uptake, and triiodothyronine by radioimmunoassay (t3[r.i.a.]). radioactive-iodine uptake (r.a.i.u.) was suppressed in all. in all patients, thyrotoxicosis resolved within 4 months; subsequent transient hypothyroidism occu ...	1977	68224
t4 radioimmunoassay with ion-exchange resin separation.		1977	68243
effect of insulin-induced hypoglycemia on the level of serum tsh, total t4, free t4 and t3.		1977	68540
sporadic non-toxic goitre. a long-term follow-up of 36 patients.	thirty-six patients with non-toxic goitre were reviewed after a mean period of 13 years. initially 19 patients were iodine deficient (group i) and 17 had normal plasma inorganic iodine (group ii). in general the iodine deficient patients had larger goitres, developed more complications (26% became hyperthyroid, hypothyroid or required partial thyroidectomy), and required more treatment. there were marked changes in thyroid function tests. in both groups thyroid uptake fell and pii rose though th ...	1977	68654
combined therapy of oral cancer bleomycin and radiation: a clinical trial.	a concurrent randomised controlled clinical trial to evaluate a combination of bleomycin and radiation as against radiation only in the treatment of advanced oral cancer has been conducted at the cancer institute, madras, since 1971. all t3 and t4 previously untreated oral squamous cell carcinomas with n0, n1 and n2 regional nodes, or n3 nodes confined to the submandibular region, without systemic metastases or gross infiltration of the temporal and infratemporal fossa producing total trismus, a ...	1977	68844
diagnosis of hyperthyroidism when serum-thyroxine alone is raised.	31 patients admitted to hospital with different non-thyroidal intercurrent diseases were found to have raised total serum-thyroxine (t4) and free t4 together with normal serum-triiodothyronine (t3) concentrations. at admission none was clinically hyperthyroid. patients were assigned to 3 groups according to clinical course and the laboratory findings. in the first group (14 patients) classic hyperthyroidism developed after recovery from the intercurrent disease. 11 out of these 14 patients had r ...	1977	69145
spectrum of thyroid function in patient's remaining in remission after antithyroid drug therapy for thyrotoxicosis.	thyroid function was assessed in 110 patients who were in remission for a period of 7-6 +/- 0-6 years (mean +/- s.e.) (range 0-25--25 years) after the withdrawal of antithyroid drug therapy for thyrotoxicosis. on the basis of clinical examination and on the results of thyroid-function tests, the following group of patients were identified: (i) euthyroid with normal plasma-total thyroxine (t4) and triiodothyronine (t3) concentrations, but an absent or subnormal response of plasma-thyrotrophin (t. ...	1977	69792
role of urinary solutes in natural immunity to gonorrhea.	natural resistance of the male urethra to gonococci has not been explained by classical immune mechanisms but could result from antibacterial properties of urine. accordingly, we measured survival in midmorning urine of 10(7) f-62 t2 gonococci per ml by serial dilutions and plate counts. fifteen killer urines from eight people all killed greater than 3 logs (average, 5.3), and 13 of 15 were sterilized. fourteen nonkiller (inhibitor) urines from seven subjects allowed no growth. killer urines wer ...	1977	13038
endonuclease of t4 ghosts.		1977	13531
the two dispensable structural proteins (soc and hoc) of the t4 phage capsid; their purification and properties, isolation and characterization of the defective mutants, and their binding with the defective heads in vitro.		1977	15127
equimolar addition of oligoribonucleotides with t4 rna ligase.	t4 induced rna ligase will join equimolar concentrations of two oligoribonucleotides, (ap)3c and p(up) 5, to form a single product, (ap)3cp(up) 5, in high yield. the presence of the 3' phosphate on p(up)5 prevents the oligomer from adding to itself. the ph optimum of the reaction is about 7.5, but less of the undesirable adenylated intermediate, app(up) 5, forms at ph 8.2. the reaction rate is a linear function of oligomer concentration from 3 micronm to 0.6 mm. the data suggest that t4 rna lig ...	1977	17097
properties of the thyroxine (t4) monodeiodinating system in rat liver homogenate.	the monodeiodination of t4 in rat liver homogenate was studied. the two possible products of this reaction show very different properties. the metabolically very active t3 is rather stable in this system whereas the biological inactive reverse t3 (rt3) disappears very rapidly. this explains the low apparent rt3 production in the incubation mixture even under optimal conditions and the peculiar ph profile. the t4 to t3 converting reaction can be increased by the addition of mercaptoethanol to the ...	1977	18299
conversion of t4 to t3 and rt3 and their cytoplasmic binding: ph dependency.	the fate of t4 in target organs is conversion to t3 which is mainly responsible for the nuclear action of thyroid hormones. to further investigate this converting step the increase of t3 and its analogue rt3 in rat liver microsomes was measured by specific radioimmunoassays after adding t4 to the incubation medium. maximal increase of t3 occurred at ph 6.0 and of rt3 at ph 9.5 indicating that two different enzymic systems may be involved in the deiodination of the tyrosyl or the phenolic ring of ...	1977	18300
a study of extrathyroidal conversion of thyroxine (t4) to 3,3',5-triiodothyronine (t3) in vitro.		1977	18337
purification and properties of a t4 bacteriophage factor that modifies valyl-trna synthetase of escherichia coli.	after t4 bacteriophage infects escherichia coli, a peptide tau, produced under the control of a phage gene, binds to the host valyl transfer ribonucleic acid synthetase (ec 6.1.1.9) and thereby changes several of its physicochemical properties. the interaction of tau with the host enzyme was investigated in vitro after extensively purifying the factor from t4-infected e. coli using a rapid purification procedure. the tau preparation migrated as a single, protein-staining band with a molecular we ...	1977	19475
analysis of the activity of micrococcus luteus endonucleases with respect to gamma-irradiated dna.	endonucleases from micrococcus luteus that induce single-strand breaks in gamma-irradiated dna have been separated chromatographycally into two groups. the first group involves two different enzymes: ap-endonuclease ii (mol. weight 30 000) and ap, uv-endonuclease i (mol. weight 15 000) that recognize alkali-labile lesions in gamma-irradiated dna and apurinic sites in dna heated at 70 degrees c, ph 6.08 ap-endonuclease ii in cooperation with dna polymerase from m. luteus and t4 phage-induced poly ...	1977	20161
assembly of bacteriophage t4 head-related structures. assembly of polyheads in vitro.		1977	20510
characterization of human thyroxine-binding globulin. evidence for a single polypeptide chain.	thyroxine-binding globulin (tbg) was purified from fresh human plasma by affinity, anion exchange, and gel filtration chromatography. the protein gave a single band in overloaded analytical disc gel electrophoresis. the molecular weight was 54,000 and e1%/1 cm at 280 nm, corrected for thyroxine (t4) absorbance, was 6.17. six preparations of tbg contained from 0.09 to 0.64 mol of t4/mol; the tbg used in this study contained 0.19 mol of t4 and was able to bind an additional 0.85 mol. the carbohydr ...	1977	21881
the effect of thyroxine on the 2,3-diphosphoglycerate content of erythrocytes in vivo and in vitro.	after ending a continous treatment with thyroxine the average dropping of the 2,3 dpg level was 0.4 mumol/ml. t4 decreased on the average by 7.6 microgram/ml. one time application of 1 mg thyroxine p.o. led within 24 hours to an increase of the 2,3 dpg level of -chi = 0.2 mumol/ml, the ph in the erythrocytes increased by 0.02 on the average. blood incubation with thyroxine added in a concentration of -chi = 24 microgram/100 ml showed no increase of 2,3 dpg, ph and phosphate, while there was a si ...	1977	23631
concentration of thyroxine-binding globulin: value of direct assay.	the concentration of thyroxine-binding globulin (tbg) in the serum can now be measured by direct assays that are simple and inexpensive. comparison of a direct measurement of tbg concentration with a widely used indirect method (thyopac-3) showed that the indirect method was inaccurate when tbg concentrations were high. this will result in an increase in the derived free thyroxine index (fti), so that euthyroid patients with a raised tbg concentration may be at risk of being labelled thyrotoxic. ...	1977	65198
the inhibitory action of the pineal extract on trh.	the effects of a single dose and of 5 doses (5 days) of a bovine polypeptide pineal extract (pe) on the pituitary tsh reserves and on the blood level of thyroid hormones were studied on hypothyroid patients. the blood levels of pbi, total t4 (radiocompetitive), free t4, t3 and tsh (ria) in basal conditions and during the trh test (i.v. administration of 200 mug trh), in the presence and absence of the treatment with pe were determined. thirty minutes following i.m. administration of pe a decreas ...	1977	66735
homocytotropic antibodies (ige) to neisseria gonorrhoeae in the rat and a cross-reactivity of heterologous gonococcal strains.	outbred wistar rats were immunised with a single intraperitoneal injection of a mixture of 30 mg of a1(oh)3 and 100microng of gonococcal zeolite antigen (za). ten days after immunisation, za prepared from t1 and t4 colonies of neisseria gonorrhoeae strain gc6 (gc6-t1 za and gc6-t4 za) were able to elicit a reaginic (ige) response which declined to low levels by 35 days. there was a significant amount of histamine release from the mast cells of actively-sensitised rats on challenge with a specifi ...	1977	66964
[comparison between the ria-t4 and the t4 (sebnitz) tests].		1977	74134
[relationships between streptococcus pyogenes t4-antigens and haptoglobin types (author's transl)].	some strains of beta-haemolytic streptococci were agglutinated with high titers (1:200-1:6400) by human sera of the haptoglobin types 2-2 and 2-1. no agglutination or with low titers (up to 1:20) was observed with sera of the haptoglobin type 1-1. this reactivity depends from the contents of streptococcus pyogenes t-antigens of the t-complex 4,24... this t4-antigen is present in group a streptococci (strep. pyogenes) of the types 4, 24, 26, 28, 29, 46, 48 and 60 but it may be present also in som ...	1977	74924
effect of pregnancy on thyroxine binding globulin (tbg) in partial tbg deficiency.	an unusual opportunity was afforded to study the effect of endogenous increase in estrogen on thyroxine binding globulin (tbg) throughout pregnancy in a partially tbg-deficient female who conceived subsequent to initial examination. tbg binding of 125i-thyroxine (t4) before and up to four months of pregnancy was low in comparison to normal. starting from six months and up to the end of pregnancy, tbg activity showed a definite increase although still below normal. tbg binding capacity increased ...	1977	74952
[hormone concentrations in thyroid gland tissue and plasma in autonomous thyroid adenomas with and without thyrostatic pretreatment].	the thyroid hormone concentrations of t2, t3 (and the inactive r-t3) were determined in thyroid tissue of 20 patients with autonomous adenomas. high concentrations were found in scintigrafically decompensated adenomas without preoperative thyrostatic treatment. decompensated adenomas after thyrostatic treatment, compersated adenomas and a group of 9 euthyroid goiters showed no difference in tissue-concentrations of t4 and t3. the amount of tissue-r-t3 seems to be lowered in autonomy. the plasma- ...	1977	87296
[in vivo regulation by calcium of thyroid metabolism of iodine in rats].	in vivo experiments using a calcium deficient or a normal diet, proved that calcium enhance iodine entry and concentration into the thyroid gland of the rat and the intra-thyroidal t4 pool, t4 level in serum was also increased. peripheral metabolism of t4, serum tsh levels and pituitary tsh contents were unaffected. calcium has a local regulatory function on the thyroid gland.	1977	148945
thyroid function in adults with down's syndrome.	the thyroid status of 82 institutionalized adults with down's syndrome has been assessed. compared to age and sex matched control subjects, these patients had significantly lower mean total serum thyroxine (t4) and triiodothyronine (t3) concentrations (t4; 69.1+/-22.2 nmol/1; (mean+/-sd) vs. 100.1+/-19.1, p less than 0.001; t13; 1.61+/-0.47 nmol/1 vs. 1.76+/-0.34, p less than 0.025), lower free thyroxine index (fti), (fti; 66.1+/-22.4 vs. 95.1+/-20.2, p less than 0.001), and higher basal serum t ...	1977	138688
purification and properties of a dna-dependent atpase induced by bacteriophage t4.	a dna-dependent atpase formed after t4 phage infection is purified to apparent homogeneity. the molecular weight of the purified enzyme is 50 000 when determined by glycerol gradient centrifugation and by sodium dodecylsulfate/polyacrylamide gel electrophoresis. the enzyme at an earlier stage in purification (prior to deae-cellulose chromatography) exists as a complex with a molecular weight of 100000. however, molecular weight determinations by sephadex gel chromatography give considerably decr ...	1977	140803
[biosynthesis of thyroglobulin in the endostyle of larva (ammocoetes) of a fresh water lamprey, lampetra planeri b1].	the biosynthesis of thyroglobulin (tg) in larva of a fresh-water lamprey, lampetra planeri b1. has been established. this glycoprotein presents the same characters as in thyroid follicles of adult lampreys, as shown by its 18-19 s sedimentation coefficient and by the incorporation (in vivo and in vitro experiments of 4, 12, 72 h) of 125i, 3h-leucine and 3h-galactose. 3-8 s fractions and a 12 s monomer are the precursors of the 18-19 s protein. total i % of tg is very low (0.002 %) ; about 5 % of ...	1977	142547
[computerized tomography of the facial skeleton (part ii). pathological changes (author's transl)].	the value and main task of computerized tomography in the diagnosis of benign and malignant processes in the middle and upper facial skeleton lies in its accurate demonstration of the extent of the disease; this is important in relation to the planning of treatment, prognosis and to other complementary procedures. ct is a new and potent method for the tnm classification of tumours of the nose, paransal sinuses and nasal pharynx. the ability of the ct to demonstrate deeply situated structures in ...	1977	142712
in vivo responses of alloreactive lymphocytes stimulated in vitro. helper-cell activity of mlr-primed lymphocytes.	populations of mouse lymphocytes enriched in specific alloreactive cells by priming in a mixed lymphocyte response (mlr) include cells which, when injected into congenic nude mice, enable them to make alloantibody after immunization. helper cells for the priming h-2 alloantigens (h-2b or h-2k) were enriched relative to helper cells for the other h-2 type. furthermore, the alloantibody responses of nude mice reconstituted with lymphocytes primed twice in vitro were virtually monospecific for the ...	1977	143715
sucrose gradient analysis: computer simulation and measurement of the parameters involved in the sedimentation of dna molecules.	the montecarlo method is used to computer simulate a random distribution of molecular lengths generated by inducing t4 dna fragmentation through the decay of 32p atoms introduced in the molecule. taking into account the experimental conditions we find that the value of alpha for alkali sucrose gradients is 0.46 +/- 0.02 and does not depend on the running time. our findings also prove that the computer simulation can be utilized to analyze sedimentation profiles of dna molecules fragmented in viv ...	1977	143820
[the effect of mn2+ on thyroid iodine metabolism in rats].	injection into rats of manganous chloride (2 mg/100 g) showed, after a 24-hr period, a marked depression of thyroidal 131i uptake. there is a decrease in serum t3 and t4 levels and an increase in i- level. these results demonstrate that the action of manganous ions is to block uptake of i- by the thyroid.	1977	146543
in-vitro studies of normal human thyroid cells: responses to thyrotrophin and dibutyryl cyclic amp.	follicular cells isolated from normal human thyroid tissue have been cultured for up to 140 h with bovine thyrotrophin (tsh) or dibutyryl cyclic amp (dbcamp). both compounds induced marked reorganization of the cells into three-dimensional follicular structures, whilst non-supplemented cells assumed a monolayer form. cultures treated initially with tsh or dbcamp showed a greater iodide uptake capacity, in comparison with unsupplemented cultures, in which iodide uptake was markedly diminished aft ...	1977	188967
evidence for a diffusible t4 bacteriophage protein governing the initiation of delayed early rna synthesis.	two forms of prereplicative phage rna can be discerned in escherichia coli early after infection with bacteriophage t4, immediate early and delayed early rna. the transition from immediate early to delayed early rna synthesis is inhibited by chloramphenicol. the present work presents evidence for the existence of a phage-specific protein, which effects this transition. delayed early rna formation was measured by a cistron-specific enzyme-forming-capacity method, in which rna synthesized in the p ...	1977	189081
immunoassay for honey bee cytochrome c in single animals with cytochrome c-coated bacteriophages: a sensitive tool for the study of caste formation in the honey bee, apis mellifera.	the development of a sensitive viroimmunoassay for honey bee cytochrome c and its usage for early detection of caste differentiation is described. pure honey bee cytochrome c was isolated from workers and used to produce antibodies in rabbits. bacteriophage t4 was chemically modified by covalent attachment of honey bee cytochrome c using tolylene-2,4-diisocyanate as a cross-linking agent. the immunospecific inactivation of this bacteriophage-cytochrome c conjugate by anti-cytochrome c antibodies ...	1977	189841
specific action of t4 endonuclease v on damaged dna in xeroderma pigmentosum cells in vivo.	the specific action of t4 endonuclease v on damaged dna in xeroderma pigmentosum cells was examined using an in vivo assay system with hemagglutinating virus of japan (sendai virus) inactivated by uv light. a clear dose response was observed between the level of uv-induce unscheduled dna synthesis of xeroderma pigmentosum cells and the amount of t4 endonuclease v activity added. the t4 enzyme was unstable in human cells, and its half-life was 3 hr. fractions derived from an extract of escherichi ...	1977	197527
effects of dexamethasone, desoxycorticosterone, and acth on serum concentrations of thyroxine, 3,5,3'-triiodothyronine and 3,3',5'-triiodothyronine.	the effects of a pure glucocorticoid, dexamethasone, and a pure mineralocorticoid, desoxycorticosterone, on the serum concentrations of thyroxine (t4), 3,5,3'-triiodothyronine (t3), and 3,3',5'-triiodothyronine (reverse t3, rt3) were compared both in healthy subjects and in athyreotic t4-substituted patients. in addition, the effect of exogenous acth was examined in healthy subjects. both in healthy subjects and in t4-substituted athyreotic patients, administration of a single oral dose of dexam ...	1977	197800
restoration by t4 ligase of dna sequences sensitive to "flush" cleaving restriction enzyme.	fouteen "flush"-ended segments originate from the action of the restriction endonuclease hae iii of haemophilus aegiptius on the dna of the colicinogenic factor cole 1 (a. oka and m. takanami, nature, 264, 191, 1976). they are joined by the t4 polynucleotide ligase. the reaction can be monitored by gel electrophoresis, electron microscopy and resistance to phosphatase of the 5'-32p labelled ends. the joined products are a random recombination of the original segments, and can be cleaved by the s ...	1977	198743
enzyme associations in t4 phage dna precursor synthesis.	a direct approach is described to the question: are enzymes of dna precursor synthesis organized into a supramolecular structure? this approach involved sedimentation analysis of several t4 phage-coded early enzyme activities in crude lysates of infected escherichia coli. one-third to one-half of several activities tested-dcmp hydroxymethylase, dtmp synthetase, deoxynucleoside 5'-monophosphate kinase, deoxyuridine triphosphatase, and probably dcmp deaminase, but not dihydrofolate reductase or dn ...	1977	198773
inhibition by somatostatin of mouse thyroid activity following stimulation by thyrotrophin, isoprenaline and dibutyryl cyclic-amp.	the recent discovery of somatostatin-containing cells within the thyroid gland infers that somatostatin may influence thyroid activity. this possibility was investigated by measurements of radio-iodine release in mice pre-treated with 125i and t4. the animals were treated with tsh, isoprenaline or dibutyryl-cyclic amp with and without concomitant injection of somatostatin. it was found that somatostatin reduced the blood 125i increase in response to each of the three thyroid-stimulating agents. ...	1977	199013
3'-phosphatase activity in t4 polynucleotide kinase.	the purification of t4 polynucleotide kinase results in the copurification of an activity which will specifically remove the 3'-terminal phosphate from a variety of deoxyribonucleotides and ribonucleotides in the absence of atp. this phosphatase activity requires magnesium, has a ph optiumum of 6.0, and is more active with deoxyribonucleotides than ribonucleotides. t4 polynucleotide kinase and the 3'-phosphatase activity copurify by gradient elution column chromatography on deae-cellulose, phosp ...	1977	199248
purification and properties of the nad+: protein adp-ribosyltransferase responsible for the t4-phage-induced modification of the alpha subunit of dna-dependent rna polymerase of escherichia coli.		1977	199442
role of potassium in the synthesis and decay of two specific bacteriophage t4 messages.	the role of k+ in the in vivo metabolism of specific phage t4 messengers was studied. by using a mutant of escherichia coli defective in its ability to accumulate k+ from the growth medium, it was possible to rapidly deplete cells of their intracellular k+ and in this way determine k+-dependent reactions in vivo. the rate constants for accumulation, synthesis, and decay of the early enzymes deoxynucleotide kinase and alpha-glucosyl transferase were determined. it was shown that there is a very c ...	1977	199738
hormonal influences on rna and dna synthesis in developing rat brain.	in recent years, authors' interest was focussed on the role of hormones in the brain biochemical maturation during the early postnatal life. one-day-old rats were used in the investigation of the following hormones: thyroxine (t4), hydrocortisone, aldosterone and acth, in unique or repeated administration. dynamics of cerebral rna and dna synthesis, a realiable indicator of central nervous system (cns) maturation, was investigated. authors' research led to the following conclusions: in infant ra ...	1977	199914
pituitary nuclear 3,5,3'-triiodothyronine and thyrotropin secretion: an explanation for the effect of thyroxine.	an excellent correlation was observed between nuclear triiodothyronine (t3) and the ensuing suppression of thyrotropin (tsh) after a single intravenous injection of t3 to thyroidectomized (hypothyroid) rats. at 1 and 2 hours after injection of thyroxine (t4), in amounts equally potent to the administered t3 in terms of acute suppression of tsh, the same quantities of t3 were found in the pituitary nuclei. virtually no nuclear t4 was present, and plasma t3 was negligible at these short intervals ...	1977	199941
review of 18 years' experience with pituitary tumors.	the presenting signs, symptoms, roentgenographic findings, endocrine evaluations, treatment, and results in 68 cases of presumed pituitary adenomas treated over an 18-year period are discussed. the most common symptoms were headache, acromegalic changes, visual symptoms, and amenorrhea. most common physical findings were obesity, acromegaly, and visual field defects, usually bitemporal hemianopsia. roentgenographic evidence of sellar erosion was almost universal but angiography and pneumoencepha ...	1977	199948
dinucleoside pyrophosphate are substrates for t4-induced rna ligase.	rna ligase isolated from bacteriophage t4-infected escherichia coli will utilize a number of different compounds with the general structure ado-5'pp-x as substrates in an atp-independent reaction. the p-x portions of these molecules are transferred to the 3'-hydroxyl of an oligoribonucleotide to form a phosphodiester bond, and the ado-5'p (amp) portion is released. amp, cmp, gmp, ump, dtmp, nmn, alphanmn, reduced nmn, fmn, rib-5p, phosphopantetheine, and cyanoethylphosphate all have been added t ...	1977	200936
[effect of thyrotropin releasing hormone thyroxine on the activity of cytochrome oxidase in rat adenohypophysis].	thyrotropin releasing-hormone (trh) increased the activity of cytrochrome c oxidase in a concentration of 0.01 and 1.0 microgram/ml in the adenohypophysis of rats fed methylthiouracil for 6 weeks. this effect of trh on the activity of the enzyme was blocked with t4 added to the incubation medium in a concentration of 20 microgram/ml. actinomycin d (20 microgram/ml) prevented the block of the enzyme with thyroxin. in a concentration of 0.01 microgram/ml trh, and in a concentration of 2.0 microgra ...	1977	201315
inhibition of plasma cyclic amp, glucose and cortisol response to surgery by epidural analgesia.	cyclic amp, glucose and cortisol in plasma were measured in three groups of patients undergoing hysterectomy. the operations were performed under general anaesthesia, under general anaesthesia combined with epidural analgesia and under epidural analgesia alone. surgery elicited a significant rise in plasma cyclic amp, glucose and cortisol when performed under general anaesthesia alone. epidural analgesia extending from t4-6 to s5 combined with general anaesthesia abolished the rise in cyclic amp ...	1977	201331
binding of thyroxine and triiodothyronine in solubilized nuclear extract of human leukocytes.	an in vitro study was carried out of the interaction of thyroidal hormones with leukocytes and with a solubilized extract of the nuclear fraction of human leukocytes. the respective association constants characterising the binding of triiodothyronine (t3) and thyroxine (t4) to whole leukocytes are roughly equal (for t3, ka = 3.1 x 10(11) 1/mol and for t4, ka = 4 x 10(11) 1/mol) but a 0.4 kcl extract of the nuclear fraction exhibits a different affinity to t3 (ka = 2.16 x 10(11) 1/mol) in compari ...	1977	201457
thyroxine secretion by isolated hog thyroid cells: a cyclic amp independent pathway.	the release of 131i-labeled thyroxine (t4) from isolated hog thyroid cells was increased 1.5--2-fold by thyrotropin (tsh). dibutyryl cyclic amp failed to reproduce this tsh action. in this in vitro system another cell activity, t4 synthesis, was stimulated in an essentially identical fashion by tsh and dibutyryl cyclic amp (time course of action, dose-response relationship). 3-isobutyl-1-methylxanthine (ibmx), 0.5 mm, did not alter the basal [131i]t4 release whereas it enhanced the [131i]t4 synt ...	1977	201515
slow switchover from host rna synthesis to bacteriophage rna synthesis after infection of escherichia coli with a t4 mutant defective in the bacteriophage t4-induced unfolding of the host nucleoid.	most, if not all, host rna synthesis was shut off after infection of escherichia coli strain b/5 with a bacteriophage t4 multiple mutant defective in the abilities to induce (i) unfolding of the host nucleoid (unf-), (ii) nuclear disruption (ndd-), and (iii) host dna degradation (dena-, denb-). the shutoff of host rna synthesis and turn-on of phage rna synthesis were slower after infection of e. coli with unf- phage than after infection with unf+ phage. this delay in the switchover from host rna ...	1977	201776
increased response of adipose tissue of the ob/ob mouse to the action of adrenaline after treatment with thyroxin.	the activity of the lipolytic system of the obese hyperglycemic mouse was assessed after treatment with physiological doses of thyroxin (t4). the treatment significantly increased fatty acid mobilization in response to adrenaline over the levels observed in the control mice under all conditions studied. the activities of the high- and low-km phosphodiesterases and of adenylate cyclase were also studied. treatment of the ob/ob mice with t4 had little effect on the activities of the cyclic amp pho ...	1977	202380
end labeling of enzymatically decapped mrna.	a method is presented for rapid and efficient 5' end labeling with 32p of capped mrnas, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-p-atp and t4 polynucleotide kinase.	1977	202926
cultured thyroid cell adenosine 3',5'-cyclic monophosphate response to thyrotropin: loss and restoration of sensitivity to iodide inhibition.	unlike in all other thyroid preparations, exposure of dog thyroid cells in long-term monolayer culture to iodide (10(-7) to 10(-3) m for up to 19 h did not blunt the subsequent adenosine 3', 5'-cyclic monophosphate (camp) response to thyrotropin (tsh) stimulation. this lack of effect of iodide was observed even when confluent thyroid cells were "follicularized" by the action of tsh in the culture medium. preincubation of these cells in thyroxine (t4) and triiodothyronine (t3) was similarly witho ...	1977	233822
iodoamino acids in abnormal and grossly normal thyroids: comparison between 125i and 127i distribution.	patients with thyroid cyst (tc), non-toxic nodular goiter (nng), follicular adenoma (fa) and papillary carcinoma (pc) were given a tracer dose of 125i 40h prior to surgery. tissue specimens were hydrolysed with pronase and their labeled iodocompound distribution determined by paper or thin layer chromatography; they were then derivatized and their stable iodoamino acids (iaa) determined by gas liquid chromatography (glc). specific activity (sa) of mit, dit and t4 was within the same range in tc, ...	1977	267712