Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| suppressors of gene 32 am mutants that specifically overproduce p32 (unwinding protein) in bacteriophage t4. | a gene 32 amber (am) mutant, amng364, fails to grow on escherichia coli su3+ high temperatures, suggesting that the tyrosine residue inserted at the am codon by su3+ leads to a temperature-sensitive gene 32 protein (p32). by plating amng364 on e. coli su3+ 45 degrees c, several pseudorevertants were found that proved to contain a suppressor (su) mutant in addition to the original am mutation. crosses of two of these amng364su strains to am+ phage indicated that the suppressors themselves are in ... | 1977 | 833939 |
| aspects of the absorption of oral l-thyroxine in normal man. | using a double isotope method, the optimal conditions for the absorption of an l-thyroxine (l-t4) preparation were examined. in particular, the influence of food intake on l-t4 absorption was examined. as distinct from the unfavorable results hitherto obtained with l-t4 lactase mixtures, a tablet similar to a commercial 100 mug l-t4 lactose preparation showed good absorptionof 70.6% +/- 12.9% sd. in the fasting state, the l-t4 absorption of both preparations was significantly better (p less than ... | 1977 | 834138 |
| permissive role of thyrotropin on thyroid radioiodine uptake during the recovery phase of subacute thyroiditis. | serial measurements of serum triiodothyronine (t3), thyroxine (t4), thyrotropin (tsh), and 4-hr thyroidal 131i uptake were carried out in nine patients with subacute thyroiditis. in the acute phase, suppressed tsh and 131i uptake were observed simultaneously with the elevations of t3 and t4. thyrotropin-releasing hormone (trh) failed to increase tsh in all patients studied. the mean value of an increment in serum tsh was only 1.8 muu/ml during the recovery phase when 131i uptake was normal or hy ... | 1977 | 834155 |
| t4 bacteriophage-coded rna polymerase subunit blocks host transcription and unfolds the host chromosome. | t4 bacteriophage mutants selected for their ability to grow with cytosine in their dna are defective in host transcriptional shutoff and host chromosome unfolding. the host rna polymerase purifed from cells infected by such a mutant lacks a small t4-coded subunit. | 1977 | 834238 |
| sensitization of bacteriophage t4 to 60 co-gamma radiation and to low-energy x radiation by bromouracil. | 1977 | 834849 | |
| effects of total energy withdrawal (fasting) on thelevels of growth hormone, thyrotropin, cortisol, adrenaline, noradrenaline, t4, t3, and rt3 in healthy males. | ten days of total energy deprivation evoked the following endocrine changes in 12 healthy, normal-weight males: early and marked reductions and increments in the blood levels of t3 and reverse t3, respectively, with rapid returns to pre-starvation levels after refeeding; a slight and late decrease in the blood levels of t4; a minute reduction of the blood levels of tsh; a pronounced increase in the blood levels of growth hormone, but a return towards pre-exposure levels even before discontinuati ... | 1977 | 835366 |
| serum thyrotrophin determination on day 5 of life as screening procedure for congenital hypothyroidism. | in 327 newborns cord blood thyroxine (t4) was 11.8 +/- 0.4 mug/100 ml (sem) (151.9 +/- 5.1 nmol/l), and serum thyrotrophin (tsh) 6.7+/-1.0 muu/ml. variability was marked for both t4 and tsh. remeasured in the same patients on the fifth day of life, the tsh level was 3.7 +/- 1.0 muu/ml, lower than at birth (p less than 0.001), while scattering of tsh values was much smaller, with 99.4 % of values less than 12 muu/ml... | 1977 | 836071 |
| intra-amniotic injection of thyroxine (t4y to a human fetus. evidence for conversion of t4 to reverse t3. | 131i (150 mci) was inadvertently given to a woman during week 10 to 11 of her gestation. when referred, fetal size was estimated at 31 to 32 weeks' gestation. because of the potential risk of fetal hypothyroidism, an amniocentesis, with an injection of 500 mug of thyroxine, was performed weekly from week 33 until delivery. t4, t3, thyrodi-stimulating hormone (tsh), and rt3 were measured in amniotic fluid samples obtained at 33, 36, and 37 weeks. maternal serum t4 was measured on the day of deliv ... | 1977 | 836646 |
| squamous cell carcinoma of the tonsil in young adults. | squamous cell carcinoma of the tonsil is rare in people under 40 years of age. only 11 cases have been treated at the m.d. anderson hospital since 1944. their 5-year survival was decreased (14%) when compared to the total group of patients treated for squamous cell carcinoma of the tonsil (48%). young adults had higher clinical staging than the total group. ninety-one percent of the young patients had neck nodal metastasis, with 55% staged n3. of the entire group, 76% had neck nodal metastasis w ... | 1977 | 837343 |
| the place of irradiation in the management of the primary lesion in head and neck cancers. | lesions of the skin are best excised except for specific locations where surgical excision would not likely produce disease-free margins and/or would require extensive plastic repair. a control rate of 90% for t1 lesions and 80% for t2 lesions is obtained with irradiation in the squamous cell carcinomas of the upper respiratory and digestive tract. heavy smokers and/or severe alcoholics may be preferably treated surgically. the control rates in the t3 and t4 lesions are unsatisfactory with eithe ... | 1977 | 837349 |
| effect of inorganic iodide on thyroglobulin hydrolysis in cultured thyroid glands. | the process of thyroglobulin hydrolysis in mouse thyroid glands labelled in vitro was studied from 2-24 h after they had been maintained in tissue culture. the culture medium was supplemented with mononitrotyrosine to prevent deiodination of iodotyrosines. hydrolysis of labelled thyroglobulin, under these conditions, led to the release of labelled iodotyrosines and iodothyronines. the rate of formation of these compounds was measured as an index of thyroglobulin hydrolysis (th). th was markedly ... | 1977 | 837867 |
| in vivo inhibition of thyroid secretion by indomethacin. | indomethacin (ind) was administered to adult female rats to reduce endogenous prostaglandin (pg) synthesis in order to investigate the role of pgs in thyroid hormone secretin. this treatment markedly reduced thyroidal pgf levels (667.7 vs. 1822.1 pg/my, p less than .001). although the plasma tsh concentrations were normal in the ind-treated group (41.14 vs. 40.01 mug/100 ml), dramatic decreases were observed in plasma t3 (24.5 ca. 6.7 nf/100 ml, p less than .001) and t4 (5.6 vs. 0.7 mug/100 ml, ... | 1977 | 837875 |
| the importance of thyroglobulin structure in thyroid peroxidase-catalyzed conversion of diiodotyrosine to thyroxine. | we have previously demonstrated that thyroid peroxidase (tpo) not only catalyzes the iodination of thyroglobulin and other proteins, but that it also catalyzes the intramolecular conversion of dit residues to t4 (coupling reaction). the present study was designed to determine whether the native structure of thyroglobulin contributes to the efficiency of tpo-catalyzed coupling. two lines of evidence are presented in support of the view that the conformation of thyroglobulin is important for tpo-c ... | 1977 | 837878 |
| serum thyrotropin and thyroid hormone levels in humans receiving chronic potassium iodide. | serum thyroxine (t4), triiodothyronine (t3) and thyrotropin (tsh) concentrations were measured in 13 patients with chronic obstructive pulmonary disease receiving saturated solution of potassium iodide (sski). all had a low serum t4 and a high serum tsh concentration. however, serum t3 levels were normal in eight. recovery of normal thyroid function was observed in each of the seven patients in whom the iodide was discontinued. the same hormones were measured in four normal subjects who received ... | 1977 | 838843 |
| a variant of iodotyrosine-dehalogenase deficiency. | three siblings (products of consanguineous marriage) affected with iodotyrosine-dehalogenase deficiency (presumed homozygotes) were found to have low thyroxine and large multinodular goiters, but none was mentally retarded. iodide therapy corrected the serum t4 and thyroidal iodide uptake and discharge curve. the goiters shrank with iodide treatment. the subjects demonstrated significant ability to deiodinate intravenously injected l-mono-iodotyrosine (mit) but not l-diiodotyrosine (dit); 9.9% a ... | 1977 | 838849 |
| simultaneous measurement of triiodothyronine and thyroxine in unextracted serum samples using a double-tracer radioimmunoassay method. | a method is described for the simultaneous measurement of triiodothyronine (t3) and thyroxine t4) in 0.04 ml of unextracted serum. antibodies were prepared by immunization of rabbits with t3 and t4 conjugated with human serum albumin. bound and free labeled hormones were separated by the double-antibody technique, and 8-anilino-1-naphthalene sulfonic acid was used to inhibit binding of the two hormones to thyroxine-binding globulin. the validity of the assay using 125i-t3 and 131i-t4 is shown by ... | 1977 | 839280 |
| specific innervation of guinea-pig superior cervical ganglion cells by preganglionic fibres arising from different levels of the spinal cord. | 1. the synaptic contribution of preganglionic nerve fibres arising from the last cervical (c8) and the first seven thoracic spinal cord segments (t1-t7) to neurones of the guinea-pig superior cervical ganglion has been studied by means of intracellular recording during ventral root stimulation in vitro. 2. the majority of neurones received innervation from the middle segments (t2 and t3) of the length of spinal cord from which preganglionic fibres derive; an intermediate number of ganglion cells ... | 1977 | 839468 |
| residual carcinoma of the larynx. | two hundred forty-nine patients with squamous cell carcinoma seen and treated at universtiy of wisconsin hospitals from 1960-1972 were analyzed as to recurrence rates, with emphasis on specific sites of recurrence, salvage rates, and end results. each case was carefully staged according to the tnm classification as set forth by the american joint committee for cancer staging and end results reporting (revised, 1972). of the cases treated initially with radiation, 58 percent developed recurrences ... | 1977 | 839920 |
| serotypes of pasteurella haemolytica in ovine pasteurellosis. | the serotypes of 406 strains of pasteurella haemolytica were determined. these came from cases of pasteurellosis in sheep of all ages. serotype a2 was the most frequent and comprised 33 per cent of all strains recovered. serotypes t3, t4 and t10 comprised 16, 14 and 12 per cent of the total respectively. serotypes a1 and a6 contributed 5 per cent each, a7, 18, a9, a11 and a12 a total of 8 per cent collectively, serotype a5 was not isolated at all while 6 per cent of strains were serologically un ... | 1977 | 841198 |
| elevated triiodothyronine and dextrothyroxine levels: a potential cause of iatrogenic hyperthyroidism. | a woman in whom the use of dextrothyroxine was associated with clinical hyperthyroidism is described. she had a markedly elevated t3 level while receiving the drug, apparently resulting from conversion of the dextrothyroxine to triiodothyronine. the t3 levels fell to normal after withdrawal. patients receiving dextrothyroxine should be examined for possible elevation of t4 and t3 thyrotoxicosis. | 1977 | 841413 |
| structural aberrations in t-even bacteriophage. viii. surface morphology of t4 lollipops. | 1977 | 841852 | |
| enterocolitis with peritonitis in a child with pheochromocytoma. | an 8-year-old boy presented at ucla hospital with a one month history of hypertension prior to suffering a sudden onset of acute abdominal pain, rectal bleeding, peritonitis, and shock. sigmoidoscopy showed diffuse mucosal friability. at laparotomy, inflammation and edema of the entire colon and terminal ileum were detected with two necrotic areas on the cecum. a 5 cm right adrenal pheochromocytoma with a hemorrhagic center was removed and a diverting loop ileostomy with inversion of the necroti ... | 1977 | 843139 |
| plasma and pituitary thyrotrophin (tsh) in severe and prolonged hypothyroidism, as studied in the rat. | thyroidectomized rats, kept on a low iodine diet, were killed at 60, 80 and 270 days after thyroidectomy and plasma and pituitary tsh levels measured. pituitary tsh content was lowere in the thyroidectomized rats than in the controls at 60 and 80 days, starting to increase between 60 and 80 days, and reaching higher values than those of the controls by 270 days. plasma tsh was higher in the thyroidectomized rats than in the controls at all the times studied, but declined markedly between 60 days ... | 1977 | 844217 |
| complete aimino acid sequence of bacteriophage t4 internal protein i and its cleavage site on virus maturation. | 1977 | 845944 | |
| identification and perperties of bacteriophage t4 capsid-formation gene products. | 1977 | 845961 | |
| echocardiogram before and after subtotal thyroidectomy for hyperthyroidism. | myocardial contractility was estimated in 27 patients with hyperthyroidism (10 men and 17 women, aged from 16 to 38) by echocardiography. echocardiographic examination was performed before and on the 7th day after the subtotal thyroidectomy. thyroid functions (pbi, t3, and t4) decreased significantly after the operation (p less than 0.01). heart rate and systolic blood pressure showed a slight decrease (n.s.), while diastolic blood pressure showed a slight increase (n.s.), while diastolic blood ... | 1977 | 846046 |
| [131i therapy in hyperthyroidism. results of treatment from 1960-1974]. | 488 patients with graves' disease were treated by 131iodine between 1960 and 1974. 427 (87,5%) of these patients were reexamined several times (clinically, 131i-uptake, pb127i, t4 (cpb-a), t3-uptake, and since 1973 trh-test). the 131i was given as an individually calculated single dose treatment, using 7 000 -- 10 000 rd before 1965 and 6 000 rd thereafter. two thirds of the patients became euthyroid after a single 131i-dose. in 20% the treatment had to be repeated. these patients show evidently ... | 1977 | 846862 |
| [blood plasma content of thyroxine and thyrotropic hormone in cushing's syndrome and juvenile dyspituitarism syndrome]. | the radioimmune method was applied to determination of the thyrotropic hormone (tth) and thyroxin (t4) in the plasma of 20 patients with itsenko-cushing's disease and 12 patients with the syndrome of pubertal-juvenile dyspituitrism. the plasma thyroxin level proved to be lowered in the patients with itsenko-cushing's disease (3.1 +/- 0.9 gamma%); it was normal in the patients with the syndrome of pubertal-juvenile dyspituitrism (7.8 +/- 0.9 gamma%). the plasma tth content gradually decreased in ... | 1977 | 846975 |
| chemical synthesis of restriction enzyme recognition sites useful for cloning. | by a triester chemical synthesis method, three decameric dna's have been made; these act as substrates for several restriction endonucleases, including eco ri, bam i, and hind iii. these homogenous decamers form duplexes that can be efficiently blunt-end ligated to themselves or to other dna molecules by the action of t4 dna ligase and thus are useful tools for molecular cloning experiments. | 1977 | 847463 |
| human globin messenger rna: importance of cloning for structural analysis. | the sequence of most of the human beta globin messenger rna and large sections of the alpha globin messenger rna has been determined. partly because of genetic polymorphism, it was necessary to clone globin complementary dna in order to extend the analysis. purified human fetal globin messenger rna was isolated and used as a template by reverse transcriptase to produce duplex complementary dna molecules. these molecules were linked in vitro to plasmid dna by use of t4 ligase in the presence of e ... | 1977 | 847468 |
| factors affecting t3 and t4 proficiency testing. | problem areas within a proficiency testing (pt) program are performance evaluation and sample stability. the different units used in the various t3 uptake methodologies make performance evaluation complex. to facilitate this evaluation, a normalization method for t3 uptake performance evaluation has been developed. sample stability studies for t3 uptake indicate that, at room temperature, sample values increase after storage for about seven days. room temperature sample stability studies for t4 ... | 1977 | 848501 |
| lethal and mutagenic effects of 5-iodouracil on bacteriophage t4td8rii. | evidence was obtained which indicates that the lethal effect of 5-iodouracil (iura) on bacteriophage t4 is not due to a mutagenic process. t4td8rii (thymine requiring, rapid lysis) double mutants were constructed. reversion of t4td8rii to r(+) was measured. first, reversion by growth in the presence of the structural analogues chlorouracil (clura) and bromouracil (brura) did not correlate with their relative lethal effects (for mutagenesis: iura </= clura </= brura; for lethality: clura < brura ... | 1977 | 848938 |
| saccular mucocele and laryngeal cancer. | saccular mucoceles are a definite entity and probably occur more frequently than is supposed. confusion with internal laryngoceles is possibly due to lack of understanding of basic anatomy and physiology. a 49-year-old man had a saccular mucocele that was possibly associated with laryngeal cancer, although a definitive diagnosis was only made two years later. the laryngectomy specimen was serially sectioned and showed an internal laryngocele on the side that was not operated one. this patient re ... | 1977 | 849201 |
| a new method for the assessment of thyroxine metabolism in the rat. | a new method for assessing thyroxine (t4) metabolism in the rat has been devised and the results that it yields have been compared to those obtained by other methods for assessing peripheral t4 metabolism. the new method uses a simple analysis of the kinetics of exogenous t4 metabolism in rats chronically treated with daily injections of exogenous labeled t4 to determine t4 clearance rates via both fecal and deiodinative pathways. fecal clearance rates of endogenously labeled hormone measured in ... | 1977 | 849734 |
| extrathyroidal conversion of thyroxine to 3,3',5'-triiodothyronine (reverse-t3) and to 3,5,3'-triiodothyronine (t3) in humans. | in order to estimate the relative magnitude of the two alternative pathways of monodeiodination of thyroxine (t4) in adult humans, the metabolic clearance rates (mcr) and production rates (pr) of 3,3',5'-triiodothyronine (reverse-t3,rt3) and of 3,5,3'-triiodothyronine (t3) were determined in six euthyroid control subjects (c) and in five hypothyroid patients (h) receiving l-t4 as replacement therapy (0.15-0.3 mg/day). mcr was computed by a non-compartmental method of analysis from the plasma dis ... | 1977 | 849983 |
| [pelvic phlebography by injection of contrast medium into the profundal dorsal penis vein for evaluation of tumor spread in cancer of the urinary bladder and prostate (author's transl)]. | 1. tumors with infiltration of the mucosa and submucosa (t1) as muscularis (t2) do not cause anatomical alterations of visceral and parietal pelvic veins, as numerous phlebographies have confirmed. internal pudendal veins and vessels of the bladder and prostate are involved in all patients with infiltration of bladder tumors into the paravesical tissue or prostate. -- 2. phlebographic signs for metastatic lymph node involvement in carcinoma of the urinary bladder were observed in 24.5% (t2 in 11 ... | 1977 | 850741 |
| serum triiodothyronine: clinical experience with a new radioimmunoassay kit. | a new kit for measuring total serum triiodothyronine (t3) by radioimmunoassay ('t3 ria'; radiochemical centre, amersham) was evaluated using sera from 1114 patients and normal controls. the kit performed reliably with intraassay and interassay variability figures of 3.9% and 9.3%, respectively, at 'medium' concentrations of t3. a t3 measurement could be obtained conveniently within 18 h after overnight incubation at room temperature. there were no critical steps dependent on time or temperature ... | 1977 | 856505 |
| quantitative electromyographic studies of back muscle activity relatated to posture and loading. | the myoelectric activity of some of the posterior muscles of the back was studied quantitatively using signal amplitude estimation and power spectrum analysis. surface electrodes were placed on both sides of the trunk at t4, t8, l1, l3, and l5 levels. four different angles of forward flexion were studied during external loading of the spine with 200 n. at 30 degrees of forward flexion studies were made when the load was increased from 0 to 300 n. asymmetric loading was studied with the trunk ere ... | 1977 | 857228 |
| [state of iodine metabolism in patients with temporal lobe epilepsy]. | using a radioisotope concurrent analysis the authors studied the concentration of general thyroxine (t4), the coefficient of effective thyroxine, the equivalent content of free thyroxine in the blood serum and the fixating capabilities of plasma protein in relation to t3 in patients with temporal epilepsy. the study depicted complex changes of the hormonal activity of the thyroid gland. considering the amount of the index of free thyroxine and coefficient of effective thyroxine, the state of the ... | 1977 | 857529 |
| studies on gene control regions. 2. enzymatic joining of chemically synthesized lactose operator deoxyribonucleic acid segments. | the t4 polynucleotide ligase catalyzed joining of six chemically synthesized deoxypolynucleotides corresponding to lactose operator dna has been investigated. joining was studied using various combinations of segments. joining reactions involving multiple sites and the formation of duplex operator dna were complete in a few hours. joining reactions involving a single site and the formation of only one strand of operator dna required several days and repeated annealing in order to go to completio ... | 1977 | 857879 |
| [macromolecular organization and biochemical characteristics of the genome of phage fi-1]. | the molecular weight of phage fi-1 dna is determined by the methods of sedimentation and kinetics of reassociation, as well as from buoyant density values of virion components and specific partial volume of dna (85x10(6) daltons). the spectral analyses showed that the distribution of guanine-cytosine pairs along the whole length of the molecule is gaussian. the dna content in the particle of fi-1 makes up to 41%, which is 7% less as compared to the morphologically related phage t4. the protein c ... | 1977 | 861307 |
| the organization and repair of dna in the mammalian chromosome. ii. a gradient-shape-induced speed dependence affecting dnas larger than t4 dna. | 1977 | 861366 | |
| acute effect of inorganic iodide after 131i therapy for hyperthyroidism. | patients treated with inorganic iodide weeks to years following 131i therapy for hyperthyroidism do not adapt to its antithyroid effect. to determine whether such adaptation occurs soon after 131i therapy, serum thyroxine (t4) and triiodothyronine (t3) concentrations were measured daily for 9-14 days following 131i therapy in seventeen hyperthyroid patients. nine patients received 150 mg ki daily starting 48 h after 131i administration; eight received only 131i. serum t4 and t3 concentrations di ... | 1977 | 862209 |
| enhanced thyroxine metabolism and high uptake goiters in rats after a single dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin. | nine days after treatment of rats with a single dose (25 microgram/kg) of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd), the 1 h biliary excretion of [125i]-thyroxine (t4) was increased 4-fold and the [125i]t4 bile:plasma ratio and the biliary clearance rate of plasma [125i]t4 were increased 10-fold. the proportion of biliary 125i present as t4-glucuronide was larger in tcdd-treated rats. unexpectedly, tcdd did not influence the 30 min biliary excretion of [125i]triiodothyronine (t3), the [125i]t3 ... | 1977 | 862558 |
| interaction of bacteriophage t4 rna and dna ligases in joining of duplex dna at base-paired ends. | the joining of duplex dna at base-paired ends by bacteriophage t4 dna ligase was confirmed using either a synthetic duplex decamer or restriction endonuclease fragments of cole1 dna as substrates. the reaction was not linearly dependent on enzyme concentration but increased markedly at high enzyme concentrations. although t4 rna ligase did not catalyze this blunt end joining, it makedly stimulated the dna ligase reaction particularly at low dna ligase concentrations. the apparent km for the deca ... | 1977 | 863910 |
| morphogenetic core of the bacteriophage t4 head. structure of the core in polyheads. | 1977 | 864705 | |
| precursors of the t4 internal peptides. | the precursors of the two t4 internal peptides have been identified by in vitro cleavage of individual phage proteins eluted from sodium dodecyl sulfate-acrylamide gels. the precursor of internal peptide vii is p22, the product of t4 gene 22 and an essential component of the morphogenic core. the precursor of peptide ii is a protein with a molecular weight of approximately 13,000, whose gene has yet to be defined by mutation. a newly detected protein of approximately 15,000 molecular weight is f ... | 1977 | 864834 |
| structural aberrations in t-even bacteriophage. ix. effect of mixed infection on the production of giant bacteriophage. | to date, the production of t-even bacteriophage with giant heads has been achieved in two ways: (i) by use of canavanine-arginine treatment of escherichia coli b cultures infected by wild-type bacteriophage (cummings and bolin, bacteriol. rev. 40:314-359, 1976; cummings et al., virology 54:245-261, 1973), which give a size distribution of giants that is phage specific (cummings et al., virology 54:245-261, 1973); and (ii) by infection with certain missense mutants of t4d gene 23 (doermann et al. ... | 1977 | 864836 |
| pellagra: an analysis of 18 patients and a review of the literature. | the clinical and laboratory features of 18 adult pellagrins are reviewed. only four patients (22%) had the full trial of dermatitis, diarrhea and dementia. dermatitis alone occurred in six(33%), dementia in five(28%) and dermatitis and diarrhea in three(17%). in one patient, dermentia was the initial sign of a relapse. steatorrhea was found in six patients and was usually associated with marked alopecia. edema without evidence of cardiac failure was present in seven patients. a diffuse increase ... | 1977 | 864902 |
| low-level dna exchanges in normal human and xeroderma pigmentosum cells after uv irradiation. | we have used a t4 endonuclease v assay method for uv-induced pryrimidine dimers in cellular dna in vivo to obtain evidence for recombinational dna exhanges after uv irradiation of normal human and xeroderma pigmentosum (xp) cells. our data indicate that the endonuclease-sensitive sites in excision-defective xp cells are removed very slowly from the irradiated parental strands and appear concomitantly in daughter strands newly synthesized during post-uv incubation. in the defective xp cells, the ... | 1977 | 865496 |
| on the classification of penis carcinoma and its 10-year survival. | with respect to the primary tumor there is no difference between the proposal of the uicc and the heidelberg version for tnm classification of the penis carcinoma. clinically the heidelberg scheme seems more practical, but there were no statistical differences between them. with respect to the prognosis for the patient, the size and localization of the primary tumor are of secondary importance. what is important is the degree of tumor spreading in the lymph system. from this point of view, one ... | 1977 | 866784 |
| genetic evidence for the existence of partial replicas of t4 genomes inactivated by irradiation under ultraviolet light.?*ultraviolet rays. | 1977 | 867812 | |
| seasonal variation in serum t3 and t4 levels in man. | in a group of 13 healthy male adults in the netherlands, a seasonal variation in circulating serum thyroxine (t4) and triiodothyronine (t3) levels was found, inversely correlating with the seasonally altering environmental temperature. lowest serum t4 and t3 levels were found in the summer. | 1977 | 870521 |
| polyacrylamide gel as a medium for dna dissociation and reassociation. | several properties of thermal denaturation and renaturation of dna in polyacrylamide gels were investigated: (1) following electrophoresis the dna band was scanned and shown to increase in absorbance with increasing temperature. the increase was proportioned to dna concentration across the peak. (2) the dependence of the tm on salt concentration over a hundred fold range was similar to that found for dna in free solution. (3) denaturation of several dna samples ranging in g + c content from 26 t ... | 1977 | 870817 |
| measurement of free thyroid hormones in serum by dialysis and gas chromatography. | we describe a simultaneous, absolute analysis for free (dialyzable) triiodothyronine and thyroxine in serum by the sequence: dialysis, internal standard addition to the dialysate, cation-exchange chromatography, derivatization, and gas chromatography with electron capture detection. the detection limit is 0.2 pg and the precision (repeatability) in the range of 4 to 20 ng/liter is 1.6%. mean values for free t3 and t4 in 29 euthryoid sera were 4.2 and 18.9 ng/liter, respectively, in close agreeme ... | 1977 | 872402 |
| thyroid hormone levels and protein binding in patients on long-term diphenylhydantoin treatment. | the effect of long-term diphenylhydantoin (dph) treatment on thyroid hormone concentrations and protein binding was determined in a randomized controlled trial. as has been demonstrated previously, total thyroxine (t4) concentrations were significantly depressed in patients on dph. there was no significant effect on indirect indices of protein binding of thyroid hormones, and the free thyroxine index (fti) was also significantly depressed. triiodothyronine (t3) and thyrotrophin (tsh) concentrati ... | 1977 | 872445 |
| thyroid function tests during the early phase of subacute thyroiditis. | six patients with subacute thyroditis were followed with serial measurements of t4, ft4, tsh and rai uptake. five of the six underwent trh stimulation early in the course of their illness. all six patients had elevated or high normal values for t4 and ft4 at the time of their clinical presentation (mean = 13.8 microgram per 100 ml and 3.9 ng per 100 ml, respectively). rai uptakes were 1% or less in all six. trh testing revealed a suppressed tsh response (mean deltatsh less than0.1 muu/ml) in all ... | 1977 | 874047 |
| thyroxine concentration in human milk. | thyroxine (t4) concentrations were measured by ria in 37 milk samples from 19 healthy eutryroid mothers, obtained between 3-165 days postpartum. the mean milk t4 concentration in the first week postpartum was 0.38 +/- 0.07 microgram/100 ml (mean +/- sem). the mean t4 concentrations between 8 and 48 days rose to 4.27 +/- 0.50 microgram/100 ml (mean +/- sem), and decreased to 1.11 +/- 0.25 microgram/100 ml (mean +/- sem) after 50 days postpartum. the data suggest that human milk can provide a sign ... | 1977 | 874064 |
| adaptation of tsh filter paper method for regionalized screening for congenital hypothyroidism. | a sensitive, specific, rapid radioimmunoassay is described for the determination of thyrotropin (tsh) in eluates from two 3 mm discs punched from dried blood filter-paper specimens. this method is sufficiently sensitive to easily discriminate between normal infants and infants with primary hypothyroidism. the use of two 3 mm discs enables screening laboratories to easily incorporate this methodology into the currently available, fully automated systems to screen for several metabolic disorders. ... | 1977 | 874362 |
| correlation between gestational age, weight, or ponderosity and serum thyroxine concentration at birth. | thyroxine levels were measured in cord blood sera from 2,1800 healthy infants born of "low-risk" mothers in los angeles. variations of t4 with gestational age, birth weight, ponderosity (size related to weight), sex, and race were studied, as well as the variation of w and p with ga. over the range of ga 30 to 47 weeks, serum t4 concentrations, w, and p each increase with ga, leveling off to approximately constant values from 42 to 47 weeks; the increases are 12, 66, and 20%, respectively. for a ... | 1977 | 874676 |
| genetic mapping of rega mutants of bacteriophage t4d. | sp62, a mutant of bacteriophage t4 shown by wiberg et al. (1973) to be defective in regulation of t4 protein synthesis, was shown by complementation tests to define a new gene, rega, and by intergenic mapping to lie between genes 43 and 62. the mapping involved crossing sp62 with a quadruple amber mutant defective in genes 42, 43, 62, and 44, selecting all six classes of amber-containing recombinants caused by single crossover events, and then scoring the presence or absence of sp62 in these rec ... | 1977 | 875136 |
| developmental patterns of plasma tsh, t4 and t3 in rats deprived of light from birth. | plasma levels of tsh, t4 and t3 in the rat show characteristic developmental patterns within the first postnatal month, with tsh reaching a peak on day 13, t4 on day 16 and t3 on day 20. in dark-reared rats, a significant reduction in plasma t3 associated with low plasma t4 as well as high plasma tsh suggest that light-deprivation from birth impairs and/or delays maturation of pituitary-thyroid axis. | 1977 | 875489 |
| effects of lead and cadmium on adrenal and thyroid functions in rats. | seventy sexually mature male rats were injected with lead (50 and 250 mcg), cadmium, (50 and 250 mcg) and a mixture of lead (25 mcg) and cadmium (25 mcg) for 70 days. tsr results show that 250 mcg cadmium had a stimulating effect but 1/10 of the dose of cadmium plus 25 mcg lead had the opposite effect. plasma thyroid hormones indicated that 50 mcg lead had a stimulating effect on t4 but cadmium and a mixture of lead and cadmium had a suppressing effect on both t4 and t3. plasma corticosterone an ... | 1977 | 877410 |
| [regulation of the igm immune response. ii. effect of the valence and affinity of carp anti-dnp-antibodies on the binding constant to multivalent dnp-t4-bacteriophage conjugates]. | 1977 | 878749 | |
| ventral root mapping of cardiac nerves in the canine using evoked potentials. | the sympathetic efferent contribution of left ventral roots t2, t3, and t4 to some cardiac nerves was studied using evoked potentials in mongrel dogs anesthetized with sodium pentobarbital. electrical stimulation of the ventral roots while recording evoked potentials from the sympathetic chain, anterior ansa subclavia, posterior ansa, ventrolateral cervical cardiac nerve, ventromedial cervical cardiac nerve, and the vagosympathetic trunk demonstrated that a-, b-, and c-type fibers exist in these ... | 1977 | 879297 |
| thyroxine and triiodothyronine levels in hyperthyroid patients during treatment with propranolol. | serum triiodothyronine (t3) and thyroxine (t4) levels were measured in twelve hyperthyroid patients before and after treatment with propranolol, 40 mg four times daily, for 2 weeks. there was a significant fall in serum t3 and a significant rise in serum t4 concentrations in the group as a whole and it was concluded that the clinical effectiveness of propranolol in hyperthyroidism may be mediated in part by its action on the peripheral metabolism of thyroid hormones. propranolol treatment should ... | 1977 | 880733 |
| correlation between genetic and nucleotide distances in a bacteriophage t4 transfer, rna gene. | 1977 | 881737 | |
| a case of paraparesis due to extramedullary haemopoiesis in hbe-thalassaemia. | a 24-year-old female with haemoglobin e-thalassaemia who presented with paraparesis of 18 months' duration was found to have spinal extradural extramedullary haemopoietic tissue extending from t4 to t9. complete neurological recovery followed laminectomy. the importance of prompt diagnosis and treatment, of this unusual complication of extramedullary haemopoiesis, before the spinal cord is irreversibly damaged is stressed. | 1977 | 882481 |
| duplication of colon in a labrador retriever with abnormal spinal column. | a 9-week-old male labrador retriever had duplication of the entire colon and malformation of the bodies of vertebrae t4 and t5. simultaneous occurrence of alimentary duplication and spinal abnormalities have been reported in man, and several theories have been proposed to explain these embryonic defects. | 1977 | 883088 |
| ontogenesis of thyroid function in the neonatal rat. thyroxine (t4) and triiodothyronine (t3) production rates. | 1977 | 885116 | |
| pregnancy following thyroid hormone treatment in a patient with amenorrhea-galactorrhea due to primary hypothyroidism. | a 32-year-old female with amenorrhea-galactorrhea due to primary hypothyroidism was treated with thyroid hormones, and serum levels of thyrotropin (tsh), prolactin (prl), triiodothyronine (t3), thyroxin (t4), and t3 resin sponge uptake (rt3u) were measured throughout the course of treatment. the elevated serum levels of tsh and prl fell into the normal range following t3 treatment. subsequently, the menstrual cycle was restored within 1 month, and galactorrhea completely ceased and conception wa ... | 1977 | 885277 |
| multiple interactions of a dna-binding protein in vivo. iii. phage t4 gene-32 mutations differentially affect insertion-type recombination and membrane properties. | we have investigated in in vivo roles of t4 gene-32 protein in recombination. we have studied the effects of gene-32 mutations under conditions that allow normal dna replication and are permissive for progeny production. under these conditions, certain gene-32 mutations specifically reduce insertion-type (short-interval) recombination but none affect crossover-type (long-interval) recombination (see figure 5). heterozygote frequencies in all gene-32 mutants are similar to or higher than in a gen ... | 1977 | 885343 |
| [the treatment of carcinoma of the tongue (author's transl)]. | in the past 18 years 34 patients (21 males and 13 females) with carcinoma of the tongue were treated in the author's clinic. patients with t1n0 lesions were treated satisfactorily only with irradiation. patients with advanced disease, such as t2-t4 with metastases, were classified into 2 groups. one group was treated surgically (hemiglosectomy and radical neck dissection) and postoperative irradiation (about 6000 rads). the other group was treated with preoperative irradiation (about 4000 rads) ... | 1977 | 885744 |
| depressed thyroid function in two tetraplegic dogs. | rapidly progressing tetraplegia developed in two 6-year-old dogs, both of which had eaten decayed meat sometime during the 24 hours prior to the onset of paralysis. both dogs were found to have depressed thyroid function, as indicated by extremely low triiodothyronine (t3) and thyroxine (t4) values. electromyographic studies revealed some abnormal insertional activity but no abnormal potentials when the fore- and hindlimb muscles were at total rest. nerve conduction velocity was slightly depress ... | 1977 | 885770 |
| [the results of cobalt therapy for carcinoma of the larynx (author's transl)]. | the effectiveness of cobalt therapy for carcinoma of the larynx in demonstrated by the results obtained: 53% overall survival at 5 years, with 66% for carcinomas of the glottis, and 48% for supra-glottal lésions. the dose-time ratio used was adapted to the tumor category and to the individual reactivity of the patients, local control being obtained in 100, 87, 65 and 25% for t1, t2, t3 and t4 tumours respectively. | 1977 | 886530 |
| lambda and t4 bacteriophage hybrids. | 1977 | 886622 | |
| [results of surgical treatment of cancer of the large intestine]. | the results of surgical treatment of 765 patients with colonic cancer are analysed, the resectability was 68.7%, postoperative mortality--in 16.3%. the most frequent causes of lethal issues were peritonitis and acute cardiovascular disturbances. the survival of 5 years and longer after radical operations was noted in 53.4 +/- 3.4%. the survival rate in patients with cancer of the right colon is reliably higher than that with the left colon involved. hemicolectomy yielded better results compared ... | 1977 | 888418 |
| thyroid function tests in the elderly in the community. | a study of the well elderly living at home has demonstrated that the ranges for serum thyroxine (t4), triiodothyronine (t3) uptake and free-thyroxine index (fti) are much narrower than those for the sick elderly in-patient. the prevalence of thyroid disease appears similar in both types of elderly population. | 1977 | 888746 |
| the families of patients with acute rheumatic fever or glomerulonephritis in trinidad. | the families of 21 patients with acute rheumatic fever (arf) and 44 patients with acute glomerulonephritis (agn) in trinidad were examined in their homes. the arf and agn families were equally large and crowded and they lived in the same largely rural areas. however, only 22% of the arf family members had skin infections in contrast to 61% of the agn family members. sixty-eight per cent of skin infections in arf families and 69% of skin infections in agn families yielded group a streptococci. th ... | 1977 | 888815 |
| multiple interactions of a dna-binding protein in vivo. i. gene 32 mutations of phage t4 inactivate different steps in dna replication and recombination. | 1977 | 889598 | |
| [isolation and properties of micrococcus luteus endonucleas acting in dna depurinization but inactive with pyrimidine dimers]. | an endonuclease (ap-endonuclease ii) that specifically attacks double stranded or single stranded depurinated dna, resulting in single-strand nicks, has been purified 320-fold from micrococcus luteus. the enzyme is not stimulated by 0.002 m mgcl2, it induces 3'oh-5'po4 breaks on the 5' side of apurinic sites, it has no activity towards uv-irradiated dna and has a molecular weight of about 30 000. in cooperation with dna-polymerase from m. luteus and t4 dna ligase, ap-endonuclease ii has been sho ... | 1977 | 889977 |
| the toxic effect of small iodine supplements in patients with autonomous thyroid nodules. | in sixteen cases of toxic adenoma of the thyroid (autonomous hot nodule with complete suppression of the surrounding normal parenchyma) potassium iodide was given in doses of 100 microgram/day for one week, 200 microgram/day for another and 400 microgram/day for a third week. there was a progressive increase in the serum t4 level. serum t3 also increased, although this was significant only after the first week. serum tsh was undetectable throughout the entire period of the study. this metabolic ... | 1977 | 890996 |
| bacteriophage t4 mutants which propagate on e. coli k12 but not on e. coli b. | 1977 | 891859 | |
| mutator mutations in bacteriophage t4 gene 42 (dhmc hydroxymethylase). | temperature-sensitive mutations of bacteriophage t4 gene 42 produce diverse effects upon spontaneous mutation rate. g:c vector a:t transition rates are increased, often strongly; frameshift mutation rates are weakly increased; a:t vector g:c transition rates (and perhaps also a:t vector py:pu transversion rates) are decreased; and one g:c vector py:pu transversion rate is also decreased. these results, together with certain interactions between gene-42 mutator effects and both base analogue muta ... | 1977 | 892421 |
| the evolution of epistasis and the advantage of recombination in populations of bacteriophage t4. | experiments reported here test two hypotheses about the evolution of recombination: first, the fisher-muller concept that sexual organisms respond to selection more rapidly than do asexual ones, and second, that epistasis is more likely to evolve in the absence of recombination. populations of bacterio-phage t4 were selected by the drug proflavine in discrete generations and the change in mean population fitness was monitored. three separate selection series yielded results supporting the fisher ... | 1977 | 892424 |
| [recombination in amber mutants of bacteriophage t4b. iii. influence of x-mutation on intragenic recombination in t4 phages]. | study was made of the influence of x-mutation on interallelic and intergenic recombination at the region of genes 34-38 of phage t4b. in the case of intergenic crosses in all studied recombinations of genes mutation x caused a 2-fold decrease in recombination frequency. x-mutation showed the same effect on integenic recombination in genes 36 and 38. in the case of crossing flank markers of gene 35 the frequency of recombination decreased 3.5-fold, that of gene 34--6 fold. crosses of double am-x ... | 1977 | 892435 |
| affinity labeling of human serum prealbumin with n-bromoacetyl-l-thyroxine. | affinity labeling of human serum prealbumin with n-bromoacetyl-l-thyroxine (bract4) was used to investigate the binding domain for l-thyroxine (t4) on prealbumin. fluorescence titration with 8-anilinonaphthalene-1-sulfonate revealed a strong and a weak binding site for bract4 (k1 = 1 x 10(8) m-1; k2 = 1 x 10(6) m-1). the reaction of bract4 with prealbumin to form a covalent bond was inhibited in the presence of t4 and binding of t4 to prealbumin was nearly abolished after affinity labeling with ... | 1977 | 893396 |
| thyrotoxicosis detected in a mass-screening program for neonatal hypothyroidism: demonstration of placental transfer of an immunoglobulin with marked lipolytic activity. | a female infant born to a mother with active graves disease and a strongly positive lats developed neonatal thyrotoxicosis. the diagnosis was confirmed rapidly by the markedly elevated filter paper spot t4 (5.45 ng/40 microliter) taken on the fifth day of life as part of the quebec screening program for neonatal hypothyroidism. although the infant's serum was negative for lats, it reacted strongly (as did the maternal serum) in the lipolytic assay for lats. this case illustrates the ability of s ... | 1977 | 894408 |
| bacteriophage t4 virion baseplate thymidylate synthetase and dihydrofolate reductase. | additional evidence is presented that both the phage t4d-induced thymidylate synthetase (gp td) and the t4d-induced dihydrofolate reductase (gp frd) are baseplate structural components. with regard to phage td it has been found that: (i) low levels of thymidylate synthetase activity were present in highly purified preparations of t4d ghost particles produced after infection with td(+), whereas particles produced after infection with td(-) had no measurable enzymatic activity; (ii) a mutation of ... | 1977 | 894793 |
| rabbit liver trna1val:i. primary structure and unusual codon recognition. | the major valine acceptor trna1val from rabbit liver was purified and its nucleotide sequence determined by in vitro [32p] - labeling with t4 phage induced polynucleotide kinase and finger-printing techniques. its primary structure was found to be identical with the major valine trna from mouse myeloma cells. according to the wobble hypothesis this trna, which exclusively has an iac anticodon, should decode the valine codons guu, guc and gua only. however, this trna recognizes all four valine co ... | 1977 | 896481 |
| comparative oligonucleotide fingerprints of three plant viroids. | 5' phosphorylation in vitro with gamma-32p-atp and t4 phage induced polynucleotide kinase was used to obtain rnaase a and rnaase t1 fingerprints of three plant viroids: potato spindle tuber viroid from tomato (pstv-tom), chrysanthemum stunt viroid from cineraria (chsv-cin) and citrus exocortis viroid from gynura aurantiaca (cev-gyn). these three viroids differ significantly from each other as judged from their oligonucleotide patterns. this supports the concept of individual viroid species. | 1977 | 896482 |
| [production of antibodies for t3 and t4 (author's transl)]. | 1977 | 897426 | |
| [results of radical radiotherapy in breast cancer]. | in 17790 examinations over a 5-year period, 1174 breast carcinomas have been detected and treated in the department of senology, university hospital, strasbourg, france. 467 carcinomas were treated by radiotherapy alone. five-year survival has been studied. over half of the carcinomas treated were t3 and t4-sized. overall survival is 46%. | 1977 | 897631 |
| [contribution of radiotherapy in the local-regional treatment of breast cancer]. | the role of radiotherapy in the treatment of mammary carcinoma is reviewed. the indication for postoperative radiotherapy in cases of primary tumors t2 and t3, particularly in the presence of metastatic lymph nodes (n1), is still given. for stage 1 (t1/t2 n0), postoperative radiotherapy may be omitted or can be limited to the lymphatic area which has not been surgically explored. pre-operative radiotherapy for the more advanced stage (t3/t4 and n2) has proven more effective than postoperative ir ... | 1977 | 897644 |
| [biological aspects of endemic goiter in the pyrennes atlantiques (author's transl)]. | in an endemical goiterous area (oloron sainte-marie 64, france) a thyroid investigation has been realised on a certain number of people having a goiter (g) and those without goiter (n.g.). various explorations such as t4 d, r t3 u, tsh ria, t3 ria. achilles' reflex, cholesterol have shown no significant difference nor any abnormality inspite of a global deficit in iodine (35 ng/24 h). it can only be retained an absence in negative corelation t3 tsh (p less than 0.05). | 1977 | 900873 |
| the two triiodothyronines (t3 and rt3). thyroid biosynthesis of t3 and rt3 and peripheral metabolism of thyroxine (author's transl)]. | thirty per cent of the iodine in thyroglobulin is present as iodothyronines. l-thyroxine (t4) represents 90-95% of hormonal iodine, 3,5,3'-triodo-l-thyronine (t3) contains at the most two per cent of it, 3,3'5'-triodo-l-thyronine (rt3) even less, as well as traces of 3,3'-diodo-l-thyronine. the plasma concentration of t4 is about 8 microgram per 100 ml, in the case of t3 it is 120 ng and 25 ng for rt3. the cell nucleus preferentially binds t3 and rt3 and there are also some specific mitochondria ... | 1977 | 900879 |
| synthesis and biological activity of a lambda pseudo operator. | the chemical and enzymatic syntheses of bacteriophage lambda pseudo operator dna are described. the 17 base-paired duplex contains the dna which has been proposed as the binding site for ci repressor protein. the synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six proposed operator sites in the leftward and rightward operators. however, it does not correspond exactly to any single proposed operator sequence. the chemical synthesis includes the d ... | 1977 | 901770 |
| effects of thyroidectomy and thyroxine on plasma growth hormone and insulin levels in rats. | plasma growth hormone and insulin levels were measured in normal, thyroidectomized (tx), and tx rats treated with 1-thyroxine (1-t4) or rat-growth hormone (r-gh). although normal growth was observed 5 days after surgery, a decrease of circulating levels of gh was evidenced as early as 5 days after the operation. however, the plasma insulin levels were not affected by the 5th, 10th and 15th day of thyroidectomy. with a more prolonged thyroid hormone deprivation, the plasma insulin levels of tx ra ... | 1977 | 903076 |
| localization of minor protein components of the head of bacteriophage t4. | the bacteriophage t4 capsid contains a number of minor proteins that are required for head assembly but whose detailed function and position in the head are unknown. we have found that by systematically varying the conditions of extraction, some of these minor proteins can be removed while the main capsid structure is left substantially intact. electron microscopic examination of the residual capsids showed that the extraction of the product of gene 20 is correlated with the loss of a plug that ... | 1977 | 904022 |
| properties of the nonlethal recombinational repair x and y mutants of bacteriophage t4. ii. dna synthesis. | the bacteriophage t4 recombination-deficient mutants x and y exhibited decreased rates of dna synthesis as compared to wild-type t4. mutant-induced dna synthesis was more sensitive to mitomycin c than was wild-type synthesis. however, dna synthesis in mutant- and wild-type-infected cells exhibited the same sensitivity to uv light and x-irradiation. when high-specific-activity label was administered at various times postinfection, mutant dna synthesis resembled that of wild type for 12 min. after ... | 1977 | 904025 |