Publications

thyroid function tests in patients on long-term treatment with various anticonvulsant drugs.	thyroid function tests were studied in patients undergoing long-term treatment with various anticonvulsant drugs. previous reports that diphenylhydantoin induces a decrease in the serum concentrations of total and free thyroxine (t4) and triiodothyronine (t3) without a change in the tsh concentration were confirmed. diphenylhydantoin had no effect on reverse t3. carbamazepine was also found to decrease serum t4, the free t4 index and t3 but, with the exception of t3, the decrease was smaller tha ...	1978	346267
dna breakage, repair, and lethality accompanying 125i decay in microorganisms.	effects of 125i decay in dna were investigated by measurements of strand breaks and lethal efficiencies of the decays. in bacteriophages t1 and t4, local decay effects were compared with effects of the emitted electrons by induction of both single (ssb) and double strand breaks (dsb) in the intact phage head and in extended free state dna. most dsbs were found to result from local decay effects whereas most real ssbs are caused by the electrons. a simple one-to-one relationship seems to exist in ...	1978	346313
nutritionally-induced alterations in thyroid hormone metabolism and thermogenesis.	serum 3,5,3'-triiodothyronine (t3) is increased by overnutrition and decreased by starvation. the production rate of t3 was increased by overfeeding (82%), but t4 production did not change. feeding a weight maintenance diet with high cho mimiced serum t3 and rt3 changes of overfeeding, substitution of cho by fat those of starvation. vo2 increased during overfeeding and in t4 equilibrated volunteers supplemented with 40 microgram t3 daily for 2 weeks. no weight change occurred in the latter subje ...	1978	348487
functional compartmentation of dna precursors in t4 phage-infected bacteria.		1978	348692
use of the t4 polynucleotide ligase in the joining of flush-ended dna segments generated by restriction endonucleases.	double-stranded dna segments with completely base-paired ends were obtained by the action of various restriction endonucleases on phage and plasmid dnas. these segments were joined covalently by the t4 polynucleotide ligase. the joining was monitored by the electron microscopy count of intramolecularly circularized segments. the highest extent of joining, close to 75%, was observed at 15-25 degrees c with the segments resulting from the action of the bacillus subtilis (strain r) restriction endo ...	1978	350585
[transmission of bacteriophage t4 amber mutants. i. temperature sensitivity of gene 26 t4 phage mutant amn131 multiplication in escherichia coli b cells].	when studying the single cycle of the multiplication of gene 26 mutant amn131 of phage t4, like in temperature shift experiments, the yield of this mutant in non-permissive host depends greatly on the temperature. the burts size of phage in escherichia coli b is found to be 3.3 phage particles at 25 degrees c, 1.6 at 30 degrees c, 0.051 at 37 degrees c and 0.0007 at 41 degrees c. in the case of permissive host (e. coli cr-63) the burst size per cell decreases from 158 to 49 phage particles at th ...	1978	350706
escherichia coli k-12 mutants deficient in uracil-dna glycosylase.	a new assay specific for uracil-dna glycosylase is described, escherichia coli mutants partially and totally deficient in uracil-dna glycosylase activity have been isolated by using this assay in mass-screening procedures. these have been designated ung mutants. the ung gene maps between tyra and nadb on the e. coli chromosome. t4 phage containing uracil in their dna grow on the most glycosylase-deficient hosts but are unable to grow on wild-type bacteria. this provides a simple spot test for th ...	1978	350837
genetic and physiological characterization of escherichia coli k12 mutants (tabc) which induce the abortive infection of bacteriophage t4.		1978	351929
selectivity of rna chain initiation in vitro. 1. analysis of rna initiations by two-dimensional thin-layer chromatography of 5'-triphosphate-labeled oligonucleotides.	a method for the rapid and quantitative analysis of 5'-terminal oligonucleotides of rnas made in vitro is described. the method involves synthesis of rna in the presence of [gamma-32p]atp or gtp, isolation of the rna, and digestion with t1 or pancreatic ribonucleases to release labeled 5'-triphosphate termanated oligonucleotides. the oligonucleotides are then subjected to chromatography on a polyethyleniminecellulose thin-layer system using 2 m licl, 0.01 m edta (ph 6.5) in the first dimension a ...	1978	352390
inactivation of t4 bacteriophage by near ultraviolet light in the presence of 8-methoxypsoralen.		1978	353100
in vitro construction of deletion mutants of the bacteriocinogenic plasmid clo df13.	the isolation and characterization of deletion mutants of the bacteriocinogenic plasmid clo df13 is described. to construct these deletion mutants, dna of clo df13::tn901 and clo df13-rep3::tn901 plasmids was digested with restriction endonucleases, ligated with t4 ligase and introduced by transformation into escherichia coli. the presence of the ampicilline transposon tn901 facilitated the selection of plasmids. the resulting clo df13::tn901 deletion mutants were analyzed by digestion with rest ...	1978	353730
the nucleotide sequence of threonine transfer rna coded by bacteriophage t4.	the nucleotide sequence of a low molecular weight rna coded by bacteriophage t4 (and previously identified as species alpha) has been determined. the molecule is of particular biological interest for its associated biosynthetic properties. this rna is 76 nucleotides in length, contains eight modified bases, and can be arranged in a cloverleaf configuration common to trnas. the anticodon sequence is ugu, which corresponds to the threonine-specific codons aca g. the nucleotide sequence was determi ...	1978	353731
[description of a method for measuring sera with a high level fo ige: test through the inactivation of anti-ige covered phages].	the fab fragment of an anti-ige serum was conjugated with the bacteriophage t4 through the use of glutaraldehyde. the conjugated phage was inactivated by human sera which contained ige. the degree of inactivation allowed to make a distinction between the sera of normal individuals and that of those in whom it is known that high levels of ig e exist, as in patients with allergic diseases. the method is quite sensitive, economical and does not require costly equipment. besides, the conjugated pha ...	1978	354544
further characterization of the r plasmid rts1 and its mutant ptw2: replication and incompatibility of the plasmid.	incompatibility of the r plasmid rts1 and its replication mutant ptw2 was studied in reca host cells of escherichia coli. when the r plasmid r401, belonging to the same incompatibility group as rts1, was used as a test plasmid, r401 was eliminated preferentially from (rts-r401)+ cells irrespective of the direction of transfer. in contrast, ptw2 and r401 were mutually excluded. the decreased incompatibility of ptw2 was confirmed by a direct incompatibility test in which a derivative of rts1 expel ...	1978	355218
radioimmunoassay and automated enzyme immunoassay for the determination of thyroxine.	the emit homogeneous enzyme immunoassay for the determination of thyroxine (t4) was adapted to an automatic analyser (type kem-o-mat) and compared with a conventional radioimmunoassay. sera from 92 patients whose thyrometabolic status was being examined, and control sera with 3 different t4 concentrations were analysed by both methods. day-to-day analyses of the control sera gave variation coefficients between 6 and 10% for both methods. the mean value for t4 in the patients sera was 111 nmol/l ...	1978	357675
dextran sulfates as a contaminant of dna extracted from concentrated viruses and as an inhibitor of dna polymerases.	dextran sulfate is commonly used with polyethylene glycol to concentrate viruses before extraction of their dna. however, dextran slulfate then easily contaminated such dna and acted as a potent inhibitor of dna polymerases from bacillus subtilis (iii), phage pbs2, and phage t4. dextran sulfate only weakly inhibited micrococcus luteus and escherichia coli dna polymerase i preparations.	1978	357755
bacteriophages inhibit degradation of abnormal proteins in e. coli.	on infection of escherichia coli cells by bacteriophages t4, t5 or t7, the degradation of e. coli protein fragments and abnormal proteins is inhibited. normal e. coli proteins, however, continue to be degraded at their usual rates. t4 early proteins (s) is needed to inhibit the turnover of abnormal proteins in t4-infected e. coli cells.	1978	357993
the effects of virus and host genes on recombination among ultraviolet-irradiated bacteriophage t4.		1978	358562
ionic strength perturbation kinetics of gene 32 protein dissociation from its complex with single-stranded dna.	equilibrium and kinetic studies of the interaction of gene 32 protein of t4 phage with single-stranded fd dna were performed monitoring the changes in protein fluorescence. from the fluorescence titrations, it was estimated that a monomer of gene 32 protein covered six nucleotide bases on the dna and the lower limit for the apparent association constant was 1.9 x 10(8) m-1 with a cooperative parameter of 10(3) in 0.1 m 2-amino-2-hydroxymethyl-1,3-propanediol hydrochloride (ph 7) at 25 degrees c. ...	1978	359047
glutathione-dependent enzyme reactions of the phage t4 ribonucleotide reductase system.		1978	359548
isolation of a bacterial host selective for bacteriophage t4 containing cytosine in its dna.	an escherichia coli b strain, b834 galu56, has been isolated which supports growth of bacteriophage t4 with cytosine in its dna while restricting growth of t4 with hydroxymethylcytosine. this host is partially deficient in uridine diphosphoglucose as determined by the ability of dna isolated from t4 grown on it to accept glucose in an in vitro assay. in this mutant an intact rgl restriction system recognizes unglucosylated hydroxymethylcytosine residues in phage dna, while the absence of a funct ...	1978	359832
role of lipopolysaccharide and outer membrane protein of escherichia coli k-12 in the receptor activity for bacteriophage t4.	lipopolysaccharide isolated from escherichia coli k-12 did not inactivate phage t4, although the cell envelopes with 1% sodium deoxycholate resulted in the release of cytoplasmic membrane proteins, 70% of the lipopolysaccharide, and almost all of the phospholipid. the reconstitution of phage receptor activity was achieved from deoxycholate-soluble and -insoluble fractions by dialysis against a solution of magnesium chloride. lipopolysaccharide was the only essential component in the deoxycholate ...	1978	361717
the expression of bacteriophage t4 early genes. i. four classes of patterns of expression in bacteriophage t4 early genes.		1978	361730
the expression of bacteriophage t4 early genes. ii. roles of the beta' subunit of rna polymerase and termination factor rho of the host in the expression of t4 early genes.		1978	361731
proteolysis of the major capsid protein t4 bacteriophage polyheads limited by quaternary structure.	bacteriophage t4 carrying an amber mutation in gene 22 plus an amber mutation in gene 21 form aberrant, tubular structures termed rough polyheads, instead of complete phage when they infect escherichia coli b. these rough polyheads consist almost entirely of the major capsid protein in its uncleaved form (gp23). when rough polyheads are treated under mild conditions with any of the five proteases, trypsin, chymotrypsin, thermolysin, pronase, or the protease from staphylococcus aureus v8, the gp2 ...	1978	361735
thyroid disease in relation to breast cancer.	a controversy exists in regard to thyroid function and breast cancer. hypothyroidism has been suggested as being either protective from breast cancer or predisposing to the disease. it has been hypothesized that a deficiency in circulating thyroid hormones may hypersensitize the mammary glandular epithelium toward prolactin and estrogens, thus aiding the development of breast neoplasia. on the other hand, thyroid hormone replacement therapy has been connected with an increased risk of breast can ...	1978	362055
joining of 3'-modified oligonucleotides by t4 rna ligase. synthesis of a heptadecanucleotide corresponding to the bases 61--77 from escherichia coli trnafmet.	chemically synthesized fragments corresponding to the 3' end of trnafmet from escherichia coli were joined by t4-induced rna ligase to yield a heptadecanucleotide (bases 61--77). the 3' terminus of c-c-a was modified by introduction of the ethoxymethylidene group to prevent intra- and intermolecular self-joining reactions at the 3' end. the terminal trimer was phosphorylated using polynucleotide kinase and joined to c-a-a with rna ligase. the hexamer [c-a-a-c-c-a(ethoxymethylidene)] correspondin ...	1978	363145
release of a special fraction of the outer membrane from both growing and phage t4-infected escherichia coli b.	growing escherichia coli release envelope material into the medium. upon infection with t4 phage increased amounts of this material are released and at a greater rate. in order to determine whether both inner and outer membranes are present in this material, and whether the material released by growing cells differs from that released by infected cells, we have examined the protein composition of envelope released by growing and t4-infected e. coli b. our results show: (a) the protein compositio ...	1978	363149
[lambda-t4-recombinants bearing genes 46, 45, 44, 62 of bacteriophage t4].		1978	363376
[effect of mutations in escherichia coli genes polam reca, recb and recc on the frequency of genetic recombination in amber-mutants involving the early genes of bacteriophage t4].	effects of mutations in genes pola, reca, recb and recc of escherichia coli on the recombination frequencies between rii markers of t4 have been studied in conditions of partial inhibition of some early functions. it was found that the presence of the mutations in genes pola or reca decreased significantly the recombination frequency of phage amber mutant in the gene 43 (dna polymerase), increased it in the case of amber mutation in the gene 46 (exonuclease) and had no effect on the recombinatio ...	1978	363502
[adsorptive capacity of bacteriophage mu on escherichia coli k-12 strains with deletions in the attlambda region].	escherichia coli strains with deletions in att lambda region were obtained. the comparison of the extent of deletions with the sensitivity of the corresponding mutant clones to phage mu showed that the gene controlling the sensitivity of e. coli k-12 to the phage mu is located in nad a-gal region of the bacterial chromosome. it is shown that the resistance of e. coli strains which had lost the region of bacterial chromosome between nad a gene and genes of gal-operon have adsorption character. de ...	1978	363512
[effect of semipermissive conditions on recombination involving the early genes of bacteriophage t4 in amber-mutants. i. effect of amber-mutation in gene 43].	recombination frequencies between rii markers of bacteriophage t4 under conditions of partial inhibition of several early functions of the phage by amber mutations have been studied. mutations in genes 33, 42 and 45 did not affect significantly recombination frequencies. mutations in genes 32, 44 and 46 inhibited it. it has been found that effects of double mutations in pairs of genes: 43 and 30; 43 and 52; 43 and 33; 43 and 46; 43 and 32 were consistent with theoretically expected additive eff ...	1978	363515
model for dna packaging into bacteriophage t4 heads.	the mechanism of dna packaging into bacteriophage t4 heads in vivo was investigated by glucosylation of hydroxymethylcytosine residues in a conditionally glucose-deficient host. cytoplasmic dna associated with partially packaged ts49 heads can be fully glucosylated, whereas dna already packaged into these heads is shown to be resistant to glucosylation. after temperature shift and completion of arrested packaging into the reversible temperature-sensitive ts49 head, the structure of the dna in th ...	1978	364076
non-essential uv-sensitive bacteriophage t4 mutants affecting early dna synthesis: a third pathway of dna repair.	non-essential bacteriophage t4 mutants uvs58 and uvs79 showed a lower uv sensitivity than either the excision-repair mutant v am5 or the replication-dependent recombination-repair mutant y10. the uv sensitivity of double and triple mutants carrying one of the mutations uvs58 or uvs79, and v am 5 or (and) y10 was higher than the sum of the sensitivities of the single mutants. the uvs58 mutation was mapped to the early gene region, close to amn81 (gene 41). the unirradiated mutants uvs58 and uvs79 ...	1978	364301
an escherichia coli ribonuclease which removes an extra nucleotide from a biosynthetic intermediate of bacteriophage t4 proline transfer rna.	the biosynthesis of bacteriophage t4 trnapro, trnaser, and trnaile requires enzymatic removal of extra nucleotides from the 3' terminus of the respective precursor rnas. a ribonuclease activity capable of catalyzing such reactions has been partially purified from uninfected escherichia coli using an artificial precursor rna as substrate. a number of ribonuclease activities were resolved during purification. use of e. coli strain bn, a mutant known to be deficient in the relevant ribonuclease act ...	1978	364422
effects of hormones on osteocyte function and perilacunar wall structure.	microradiographical and ultrastructural aspects of periosteocytic lacunae are determined by osteocytic activity, lacunar modeling and perilacunar miniremodeling in particular. the miniremodeling activity is constituted by two alternating processes: osteolysis and osteoplasis. modifications of lacunar shape and size and of perilacunar wall ultrastructure, caused by a direct or indirect effect of certain hormones on osteocytic activity, were observed. the lacunar modeling alterations, particularly ...	1978	365421
immunohistochemical localization of thyroid hormone in rat thyroid gland.	direct and indirect immunofluorescence techniques were used to localize the thyroid hormones triidothyronine (t3) and thyroxine (t4) in adult rat thyroid gland. optimum dilutions of the antisera were established and four tissue fixatives were investigated for usefulness in this technique. use of antibodies specific for either t3 or t4 resulted in brilliant fluorescence in the colloid pools and apical cytoplasm of follicular cells. in all cases, the adjacent parathyroid gland was devoid of fluore ...	1978	366016
characterization of the morphogenesis-dependent cleavage region of the major capsid protein (p23) of bacteriophage t4; sequence of an amber fragment of p23.		1978	366157
three-dimensional structure of thioredoxin induced by bacteriophage t4.	the three-dimensional structure of thioredoxin from bacteriophage t4 has been determined from a 2.8-angstrom resolution electron density map. phase angles for this map were determined from one heavy atom derivative and anomalous differences from cadmium in the native crystals. the molecule of 87 amino acid residues is built up from two simple folding units; a betaalphabeta unit from the amino end of the chain and a betabetaalpha unit from the carboxyl end. this structure is similar to that of th ...	1978	366603
thyroglobulin biosynthesis in a larval (ammocoete) and adult freshwater lamprey (lampetra planeri bl.).	1. the biosynthesis of 18-19s thyroglobulin has been studied in a larval and adult freshwater lamprey (lampetra planeri bl.). 2. in vivo and in vitro experiments have been performed by injecting into the coelomic cavity or by incubating branchial region labeled constituents of tg of higher vertebrates (125i, [3h]leucine and various [3h]carbohydrates). 3. larvae (ammocoetes) and adults incorporate all labels into thyroglobulin (18-19s tg), containing a small proportion of labeled t3 and t4, as id ...	1978	318323
the effect of exogenous deoxyribonucleosides on thymidine incorporation in t4-infected cells.		1978	305363
gamma-ray induced double-strand breaks in dna resulting from randomly-inflicted single-strand breaks: temporal local denaturation, a new radiation phenomenon?	the induction of single- and double-strand breaks in dna by gamma-rays has been measured. the maximum number of nucleotide pairs (a) between two independently induced single-strand breaks in opposite strands of the dna which cannot prevent the occurrence of a double-strand break was found to amount to about 16. this value did not differ significantly for the four types of bacteriophage dna investigated (t4, t7 and pm2 dna, and replicative form dna of phage phix174) and was the same in 10(-2) m p ...	1978	305424
lesions of some central nervous structures result in altered pituitary-thyroid response to acute decrease of blood thyroid hormone level.	eight groups (3--6 animals each) of rats weighing 350--400 g were subjected to electrolytic lesions of various parts of habenular, thalamic and hypothalamic areas of brain. on the 6th day after the lesion the level of blood thyroid hormone was acutely decreased with the aid of isovolemic exchange transfusion (iet) of thyroid hormone free blood suspension. the level of thyroxine (t4) in plasma was measured before and during 180 min after iet with the aid of specific radioimmunoassay and the chang ...	1978	305847
effect of thiocyanate and other univalent anions on the binding of thyroxine to rat liver nuclei and homogenate.	the binding of thyroxine (125i-t4) in rat liver homogenate and isolated nuclei was investigated in vitro. in homogenate, the binding of t4 was modified by thiocyanate ion (scn-) added to the incubation mixture as potassium thiocyanate. the displacing effect of scn- increased with increasing concentrations of this compound, the lowest effective dose being that which significantly displaced t4 from liver homogenate (105 pmol per one ml medium). moreover, scn- significantly increased the displacing ...	1978	309388
photodynamic activity of dyes with different dna binding properties ii. t4 phage inactivation.	the photosensitizing efficiency of six dyes--proflavine, 9-aminoacridine, ethidium bromide, thiopyronine, pyronine and acridine red--have been compared on the basis of the inactivation of sensitized t4 phage caused by light irradiation. this reaction was only measurable after diffusion of the dye through the phage capsid and was not observed in the presence of either chloroquine or quinacrine; it followed a single-hit kinetics as a function of the irradiation time. with each dye, a double recipr ...	1978	309450
electro-ejaculation of a complete paraplegic followed by pregnancy.	after having experimented with electro-stimulation on monkeys, the authors have put it into practice with the paraplegic. they report observations on pregnancy of a woman with a complete paraplegic below t4 who was injured 15 years ago. nineteen electro-stimulations and seven artificial inseminations were necessary. the electro-stimulation is easy and can be repeated at will.	1978	310536
cytokinetic studies in children with untreated acute lymphocytic leukaemia (all): relationship to "t" cell markers.	"the labelling index (li), mitotic index (mi) of marrow lymphoblasts and the percent of peripheral blood lymphocytes and lymphoblasts that form rosettes with sheep red cells at 4 degrees c ("t4"), were measured at presentation in 33 children with all. there was a significant correlation between li and mi, but no significant correlations between them and the total white cell count at diagnosis or the "t4" percent. three patients had greater than 55% "t4" rosettes and showed increased li and mi, a ...	1978	311412
changes of lh, tsh, gh, fsh and prl in pituitaries and sera of rats after thyroidectomy and thyroxine treatment as studied by radioimmunoassay and disc electrophoresis.	rat pituitary hormones (lh, tsh, gh, fsh and prl) were located in polyacrylamide gels after the separation of rat pituitary homogenate with the aid of electrophoresis. furthermore, the incorporation of 3h-glucosamine and 14c-leucine into various protein fractions of incubation media and pituitaries incubated for 6 h in vitro, homogenized and then subjected to disc electrophoresis was measured in six groups of rats: 1. control; 2. two weeks after thyroidectomy (tx); 3.--6. two weeks after thyroid ...	1978	311732
triiodothyronine nuclear binding in fetal and adult rabbit lung and cultured lung cells.	to investigate the possible mechanism of thyroid hormone action in the lung, we examined fetal and adult rabbit lung, and cell lines derived from lung, for specific nuclear binding sites for t3. using incubation of isolated nuclei with l-[125i]t3 at 37 c, we found approximately 2400 specific binding sites/cell in fetal lung and 1120 sites/cell in adult lung, with a similar dissociation constant (approximately 500 pm) for both tissues. the l-2 and a549 cell lines, which may have originated from p ...	1978	218786
extrapituitary regulation of the circadian rhythm of plasma corticosteroid concentration in rats.	intravascular injections of adrenocorticotropic hormone in hypophysectomized rats were approximately 3 times more effective in stimulating increases in plasma corticosteroid concentration when given at the offset rather than the onset of the daily photoperiod (12 h:12 h light-dark cycle). reversal of the photoperiodic schedule produced a reversal in the corticosteroid response after 5 days. similar temporal variations in androgen and t4 responses to lh and tsh were not obtained. although plasma ...	1978	218790
mitochondrial binding sites for triiodothyronine.	metabolic effects upon rat liver mitochondria have been observed by others within 2 min of injection of massive amounts of l-t4. reported here is the subcellular distribution of 2--5 ng high specific activity [125i]l-t3 2 min after ip injection with and without a loading dose of 30 micrograms unlabeled t3. the loading dose did not change the hormone concentration in the livers of normal or thyroidectomized rats, but it decreased the amount of hormone found in the nuclei in both groups and it inc ...	1978	218792
decreased hepatic nuclear l-triiodothyronine receptors in rats and mice bearing transplantable neoplasms.	the concentration of hepatic nuclear t3 receptors was measured in isolated nuclei from athyreotic mice bearing thyrotrophic tumors and intact rats with walker 256 carcinoma. receptor concentration was reduced in all tumor-bearing animals. the mean receptor capacity of the walker tumor-bearing rats [0.31 +/- 0.05 (sem) ng/mg dna] was significantly decreased from simultaneously assayed controls (0.47 +/- 0.04 ng/mg dna; p less than 0.01). no change in the apparent equilibrium association constant ...	1978	218807
structure of nascent replicative form dna of coliphage m13.	nascent replicative form type ii (rfii) dna of coliphage m13 synthesized in an escherichia coli mutant deficient in the 5' leads to 3' exonuclease associated uith dna polymerase i contains ribonucleotides that are retained in the covalently closed rfi dna sealed in vitro by the joint action of t5 phage dna polymerase and t4 phage dna ligase. these rfi molecules are labile to alkali and rnase h, unlike the rfi produced either in vivo or from rfii with e. coli dna polymerase i and e. coli dna liga ...	1978	272630
a cell-free assay measuring repair dna synthesis in human fibroblasts.	osmotic disruption of confluent cultured human fibroblasts that have been irradiated or exposed to chemical carcinogens allows the specific measurement of repair dna synthesis using dttp as a precursor. fibroblasts similarly prepared from various xeroderma pigmentosum cell lines show the deficiencies of uv-induced dna synthesis predicted from in vivo studies, while giving normal responses to methyl methanesulfonate. a pyrimidine-dimer-specific enzyme, t4 endonuclease v, stimulated the rate of uv ...	1978	273917
addition of mononucleotides to oligoribonucleotide acceptors with t4 rna ligase.	rna ligase induced by bacteriophage t4 catalyzed the addition of nucleoside 5',3'-diphosphates to oligoribonucleotide acceptors in the presence of atp. the reactions proceeded in equimolar concentrations of donors and acceptors. several oligonucleotides of defined sequence were synthesized by this method in yields of 28-68%. the enzyme required the phosphate ester at the 3' position of the donor molecule, nucleoside 5',2'-diphosphates being unable to serve as donors. thymidine 5',3'-diphosphate ...	1978	274717
thyroid hormone and thyrotropin levels in patients placed on colestipol hydrochloride.	bile acid sequestrant resins can bind t4 in vitro and in the gut. therefore, the effect of colestipol hcl on thyroid function was studied prospectively in 17 subjects with type ii hyperlipoproteinemia. serum t4, t3, t3 resin uptake, and tsh levels were measured before and during therapy. no change in t4 levels and only small transient, but significant, decreases in t3 were noted in a minority of patients; tsh levels did not change. small increases in t3 resin uptake were noted. we conclude that ...	1978	262768
serum 3,5,3'-triiodothyronine, thyroxine, and thyrotropin in hypothyroid infants with congenital goiter and the response to iodine.	iodine deficiency in adults caused preferred synthesis of t3; this observation has not been reported in iodine-deficient hypothyroid newborns. serum total t4, total t3, and tsh have been determined in nine full term newborns with congenital hypothyroid goiter before and after cutaneous application of iodine. the mothers of these infants had untreated euthyroid goiter and lived during pregnancy in the area of göttingen, west germany, known as an iodine-deficient region. mean total t4 in the newbo ...	1978	263290
damped oscillations in serum thyroid hormone levels of normal newborn infants.	exposure to the extrauterine environment is associated with marked stimulation of the hypothalamic-pituitary-thyroid system after which, pituitary-thyroid equilibrium must be reestablished. this marked endogenous perturbation offers the opportunity to study the manner in which the pituitary-thyroid axis is reequilibrated. t4, t3 and tsh concentrations have been measured by ria in sera from 440 healthy newborn infants, whose ages ranged from birth to 236 h. results were analyzed by nonlinear curv ...	1978	263295
metabolic clearance and production rates of 3,3',5-triiodothyronine in hyperthyroid, euthyroid, and hypothyroid subjects.	to further elucidate the peripheral metabolism of rt3 and to determine if rt3 production rates vary directly with thyroid function, we measured the disappearance of [125i]rt3 in thyrotoxic and hypothyroid subjects as well as in athyreotic patients maintained eumetabolic on exogenous t4. kinetic parameters were determined by noncompartmental analysis, and serum concentrations of rt3 and t4 were determined by specific rias. in six hyperthyroid, seven euthyroid, and six hypothyroid subjects, the mc ...	1978	263302
fetal hypothyroidism and maternal thyroid status in severe endemic goiter.	the relationship between maternal thyroid function and newborn thyroid function was studied in a region of very severe endemic goiter (ubangi, republic of zaïre). t4, t3, and tsh concentrations were measured in the sera of 56 mothers (at the time of delivery) and 60 newborns (in the cord). the results obtained in these groups (untreated) were compared with those obtained in two control groups, comprising 53 mothers whose iodine deficiency had been corrected by the injection of iodized oil and 68 ...	1978	263304
3,3',5'-triiodothyronine, thyroxine, triiodothyronine, and thyrotropin levels in maternal and cord blood sera from endemic goiter regions of brazil.	t4, t3, rt3, and tsh were measured in cord blood of infants born in mild to severe endemic goiter areas and nonendemic urban areas in south and north america. a significantly high rt3 concentration was found in newborns living in the urban area of south america as compared to a canadian newborn population. there were no differences among the various thyroid parameters in the groups of newborns studied. blood from goitrous mothers showed significantly higher concentrations of t3 and tsh than nong ...	1978	263309
effect of propranolol on extrathyroidal metabolism of thyroxine and 3,3',5-triiodothyronine evaluated by noncompartmental kinetics.	kinetic studies of t4 and t3 using a noncompartmental approach were performed in seven patients with pretreatment severe hypothyroidism maintained on l-t4 replacement. each subject received a combined tracer dose of labeled t4 and t3 as an iv bolus before and during peroral treatment with propranolol. serum t4 was unchanged, while a significant decrease of 13% was found in serum t3. the disposal rates (dr) of t4 and t3 decreased significantly, and the ratio between the dr off t3 and the dr of t4 ...	1978	263312
effect of starvation on the production and peripheral metabolism of 3,3',5'-triiodothyronine in euthyroid obese subjects.	the effect of starvation on the peripheral metabolism of rt3 was evaluated in four obese euthyroid patients. during starvation, the serum rt3 concentration increased by 69% while the mcr of rt3 decreased in all four patients from control values of 96 +/- 23 (mean +/- sd) to 68 +/- 17 liters/70 kg . day, resulting in a slight increase in the mean production rate of rt3. these findings are in contrast to the marked decrease in t3 production rate associated with fasting, indicating that inner and o ...	1978	263330
effect of intraamniotic fluid thyroxine injection on fetal serum and amniotic fluid iodothyronine concentrations.	seven hundred micrograms of t4 were injected into the amniotic cavity 24 h before delivery of five pregnant women scheduled for elective cesarean section at term. t4, t3, and rt3 concentrations were measured by ria in amniotic fluid obtained at the time of the injection and in amniotic fluid and cord serum samples collected at delivery. iodothyronine concentrations also were determined on cord samples from 24 full term control infants. the geometric mean serum t4 concentration in the experimenta ...	1978	263334
a radioimmunoassay for 3',5'-diiodothyronine.	the present report describes a ria for 3',5'-diiodothyronine (t2) that can be performed on unextracted serum and which has a lower limit of detectability of 2 ng/dl. cross-reactivity with other iodothyronines was negligible, except for rt3 which began to demonstrate cross-reactivity when rt3 levels were elevated to 180 ng/dl. employing this ria for t2, we have determined that 83 healthy individuals had a mean (+/-se) serum t2 concentration of 5.0 +/- 0.3 ng/dl, thyrotoxic subjects (n = 12) had ...	1978	263335
low serum triiodothyronine and high serum reverse triiodothyronine in old age: an effect of disease not age.	serum concentrations of t4, t3, t3, free t4, free t3, and tsh were determined in four groups of adult subjects: group a, 27 healthy young volunteers (aged 18-29 yr); group b, 24 carefully selected healthy elderly subjects (aged 70-90 yr); group c, 41 subjects living at a municipal nursing home for the elderly (aged 70-90 yr); and group d, 35 hospitalized patients (aged 70-90 yr). identical levels of iodothyronines in serum were found in the young and in the elderly healthy subjects. moderate and ...	1978	263341
effects of dexamethasone on fetal and maternal thyroxine, triiodothyronine, reverse triiodothyronine, and thyrotropin levels.	the concentrations of t4, t3, rt3, and tsh were measured at term pregnancy in maternal and umbilical plasma and in amniotic fluid of 11 normal patients who received 8-16 mg dexamethasone 3-48 h before elective cesarean section and of 10 control patients who received no dexamethasone. the mean (+/- se) concentrations of t4 (micrograms per dl) in maternal and umbilical plasma of dexamethasone-treated patients (12.5 +/- 0.9 and 13.0 +/- 0.9) were not significantly different (p less than 0.05) from ...	1978	263347
evidence that triiodothyronine and reverse triiodothyronine are sequentially deiodinated in man.	we have demonstrated that in patients given a single iv injection of t3, rt3, or, to a lesser extent, t4, all labeled with 125i in the outer or phenolic ring, chromatography of serum on columns of sephadex g-25 superfine revealed the presence of a labeled material, separate from the administered hormone and from both iodide and iodoprotein. this peak has been termed pre-t3 because it elutes just before the t3 peak. identification of the various compounds in pre-t3 was carried out by cation excha ...	1978	263472
appearance of labeled metabolites in the serum of man after the administration of labeled thyroxine, triiodothyronine (t3), and reverse triiodothyronine (rt3).	chromatography of serum on columns of sephadex g-25 superfine after the iv administration of 125i-labeled t4 consistently yielded labeled iodide, iodoprotein, t3, and a labeled peak that eluted from the column before the t3, termed "pre-t3." much larger quantities of pre-t3 were generated after the iv administration of 125i-labeled t3 and rt3. the ratio of [125i]pre t3:[125i]t3 and [125i]pre t3:[125i]rt3 plateaued at approximately 10 h and 2 h, respectively, after the iv administration of the la ...	1978	263473
difference in pituitary-thyroid feedback regulation in hypothyroid patients, depending on the severity of hypothyroidism.	in an attempt to study pituitary-thyroid interplay during replacement therapy for hypothyroidism, t4 (75-150 micrograms/day) was administered for at least 3 months. a small dose of t4 (75 micrograms/day) significantly depressed basal and trh-stimulated tsh levels in normal subjects without significantly elevating serum t4 and t3 concentrations. in patients with severe hypothyroidism and marked enlargement of the sella turcica, t4 (2.53 micrograms/kg bw) normalized the serum t4 and slightly eleva ...	1978	263732
3'-5'-diiodothyronine in health and disease: studies by a radioimmunoassay.	a simple, reproducible, and highly specific ria has been developed for measurement of 3',5'-diiodothyronine ((3',5'-t2) in unextracted serum. interference in binding of radioactive 3',5'-t2 to anti-3',5'-t2 by serum proteins was minimized by using 0.4 m phosphate buffer (ph 6.2) and merthiolate. the detection threshold of the ria was 2.5 ng/100 ml. recovery of nonradioactive 3',5'-t2 added to serum averaged 99%. t4, t3, and rt3 cross-reacted with 3',5'-t2-binding sites on anti-3',5'-t2 antibody ...	1978	263734
the effect of repeated administration of ipodate (oragrafin) in hyperthyroidism.	this report describes the effect of administration of repeated doses of ipodate (oragrafin; 3 g orally every third day for five doses) in six hyperthyroid patients. baseline serum concentrations of immunoassayable t3, rt3, and t4, were 926 +/- 206 ng/100 ml, 165 +/- 31 ng/100 ml and 21 +/- 2.7 micrograms/100 ml (mean +/- sem), respectively. within 24 h after the first dose of ipodate, serum t3 fell by 54% and it remained between 66-77% below baseline until the third day after the fifth dose; sub ...	1978	263735
circular dichroism study of the interaction between t4 gene 32 protein and polynucleotides.		1978	204331
circular dichroism studies of the interaction of a limited hydrolysate of t4 gene 32 protein with t4 dna and poly[d(a-t)].poly[d(a-t)].	gp32 i is a protein with a molecular weight of 27 000. it is obtained by limited hydrolysis of t4 gene 32 coded protein, which is one of the dna melting proteins. gp32 i itself appears to be also a melting protein. it denatures poly[d(a-t)].poly[d(a-t)] and t4 dna at temperatures far (50-60 degrees c) below their regular melting temperatures. under similar conditions gp32 i will denature poly[d(a-t).poly[d(a-t)] at temperatures approximately 12 degrees c lower than those measured for the intact ...	1978	204332
polynucleotide kinase from a t4 mutant which lacks the 3' phosphatase activity.	polynucleotide kinase from e. coli infected with the pset 1 mutant of bacteriophage t4 has been isolated. the pset 1 enzyme purifies similarly to normal polynucleotide kinase and effectively transfers the gamma phosphate of atp to the 5' terminal hydroxyl of dna and rna. the pset 1 and normal enzymes require similar magnesium ion concentrations, have the same ph optima and are both inhibited by inorganic phosphate. however, the pset 1 enzyme is totally lacking the 3' phosphatase activity associa ...	1978	205838
[in vitro thyroid hormone reception by the adenohypophysis and hypothalamus of male rats of different ages].	in studying the thyroid hormone binding with the water-salt extracts of the adenohypophysis and the hypothalamus of male rats after schreiber it was shown that with ageing there occurred in the adenohypophysis a redistribution of t4 and t3 binding in the direction fo an increase of the t4/t3 coefficient, and a reduction of the relative t3 binding, the latter bordering with the significant one. some reduction of the t4/t3 coefficient, both in comparison with the rest of the tissues under study an ...	1978	205861
growth retardation, skeletal maturation and thyroid function in children with homozygous beta-thalassaemia.	growth rate, skeletal maturation and thyroid function were measured in fifty thalassaemic children (age 2-13 years) and in fifty controls (matched for sex and age who were not anaemic. growth retardation was present in several patients during the first years of life and affected almost all subjects examined when they were approaching puberty. discrepancy between bone and height age in patients was not significantly different from controls. the finding of low t4 and t3 with a high tsh indicated t ...	1978	206391
dynamics of hormone release from the perfused canine thyroid during cyclic amp infusion.	in order to try to characterize the sequence of processes leading to hormone secretion from the stimulated thyroid, the effect of cyclic 3'5' adenosine monophosphate (camp) and related compounds were examined in 15 two-sided perfusions of canine thyroids isolated in situ. t4 and t3 concentrations in the effluent were measured radioimmunologically. camp 5 mm and tsh 100 muu per ml induced the same pattern of hormone release from the thyroid. after a latency period of 15--25 minutes a steep increa ...	1978	206500
hormone action at the membrane level. vii. stimulation of dihydroalprenolol binding to beta-adrenergic receptors in isolated rat heart ventricle slices by triiodothyronine and thyroxine.	thyroxine (t4) and triiodothyronine (t3) increase the number of beta-adrenergic receptors in heart ventricle slices. a short term effect reaches a maximum by 1.5-2 h and requires added amino acids for consistent results. the apparent km for the l-t3 effect is 15 pm. this effect, measured by an increase in stereospecific binding of (-)-[3h]dihydroalprenolol is not inhibited by cycloheximide or puromycin and is produced more effectively by l-t3 than d-t3. however, cycloheximide nearly completely i ...	1978	207354
the role of ribosomes in stabilizing bacteriophage t4 deoxynucleotide kinase mrna.		1978	208535
regulation of ribosome function following bacteriophage t4 infection.		1978	210283
dna binding site of the helix destabilizing protein gp32 from bacteriophage t4.		1978	210284
a role in true-late gene expression for the t4 bacteriophage 5' polynucleotide kinase 3' phosphatase.		1978	210287
changes in cyclic nucleotides of rat thyroid by chronic administration of lats and tsh.	the effects of lats and tsh on the cyclic nucleotide content and enzymatic activity in rat thyroid was observed during the continuous administration of lats or tsh for 6 days. serum t4 and t3 levels were increased significantly compared with the saline controls. the cyclic nucleotide (camp and cgmp) levels and enzyme activities per wet weight of tissue were determined. the thyroid weight in both the lats and tsh groups increased approximately two-fold, but camp and cgmp content per wet weight di ...	1978	210609
inhibition of t4 polynucleotide kinase by the atp analog, beta, gamma-imidoadenylyl 5'-triphosphate.	the inhibition of t4 polynucleotide kinase by beta,gamma-imidoadenylyl 5'-triphosphate has been investigated. it was found that the atp analog was a competitive inhibitor with regard to atp and a noncompetitive inhibitor with regard to dna possessing a 5'-hydroxyl group. at ph 8.0, the ki values were 3 and 11 mm, respectively. beta,gamma-imidoadenylyl 5'-triphosphate was not a substrate in the forward reaction, but would replace adp and atp in the reverse reaction. the reverse reaction was also ...	1978	210816
new late gene, dar, involved in the replication of bacteriophage t4 dna. ii. overproduction of dna binding protein (gene 32 protein) and further characterization.	we have previously shown that the arrested dna synthesis of mutant defective in t4 phage gene 59 can be reversed by a mutation in dar. in this paper, we have examined the effect of the dar mutation on the kinetics of gene 32 protein (dna binding protein) synthesis, dna packaging, progeny formation, and several other porcesses. several lines of evidence are presented showing that the regulation of synthesis of gene 32 protein is abnormal in dar 1-infected cells. in these cells, gene 32 protein, a ...	1978	211257
action of ki and several iodocompounds on [3h]uridine incorporation into thyroid rna.	the present studies were performed in order to further clarify the action of iodine and iodocompounds on the incorporation of labelled uridine into thyroid rna. ki decreased rna labelling but did not alter total [3h]uridine uptake or [3h]inulin distribution space. ki also inhibited the increase in rna labelling produced by 8 mm glucose. t4 was more potent on a molar basis than ki in impairing uridine incorporation. tetrac, triac and isopropyl-t3 also decreased rna labelling, while t2 and isoprop ...	1978	211780
purification and physicochemical properties of limited proteolysis products of t4 helix destabilizing protein (gene 32 protein).		1978	212428
synthesis of t4 dna and bacteriophage in the absence of dcmp hydroxymethylase.	several lines of research have suggested that the dcmp hydroxymethylase (hmase) coded by bacteriophage t4 is an essential protein in a dna replication complex, as well as a supplier of hydroxymethyl dcmp for phage dna synthesis. we show that a mutant [hmase, dctpase, endonuclease ii, endonuclease iv] which lacked this enzyme made cytosine-containing dna at about two-thirds of the normal rate. when coupled with an alc mutation which permitted synthesis of late proteins, a small burst of phage was ...	1978	212605
effect of thyroid hormone, actinomycin d, cycloheximide and puromycin on trh-induced secretion of tsh, as studied by pituitary concentration of cyclic amp and intrathyroidal colloid droplet formation.	in an attempt to study the functional relation between pituitary cyclic amp and tsh secretion in response to thyrotropin releasing hormone (trh) or thyroid hormone administration, pituitary concentration of cyclic amp was measured by protein binding assay after in vivo and in vitro administration of test materials (trh, thyroxine, triiodothyronine, actinomycin d, puromycin and cycloheximide singly or in combination). small dose of trh apparently augmented tsh secretion as evidenced by a marked i ...	1978	213036
thyroxine binding and deiodination of thyroxine by human lymphocytes.	binding and deiodination of t4 by lymphocytes from euthyroid, hypothyroid and hyperthyroid subjects were studied. two sets of binding sites were found on normal lymphocytes. one set with equilibrium association constant of 6.9 x 10(9) l/mol and a maximal binding capacity of 3000 mol t4 per lymphocyte, and another set with equilibrium association constant of 4.1 x 10(6) l/mol and a maximal binding capacity of 7.8 x 10(6) mol t4 per lymphocyte. the major part of the binding sites were non specific ...	1978	213211
hypothalamic hypothyroidism and hypogonadism in prolonged traumatic coma.	prolonged coma afterhead trauma is associated with depletion of 3', 5' cyclic adenosine monophosphate (camp) in the cerebrospinal fluid (csf). because camp has previously been implicated in neurorendocrine secretion, this study examines the pituitay-hypothalamic function in 15 adult male patients (to exclude the effects of puberty and menses) with traumatic coma lasting longer than 2 weeks. ventricular csf camp was measured at 2- to 4-day intervals for 10 to 25 days. simultaneously, plasma hormo ...	1978	213540
[evaluation of thyroid function in children (author's transl)].	two main methods must be used in every case for evaluation of thyroid function in children : measurement of plasma thyroxin t4 and thyroid scanning with technetium. if accurately done, they may be sufficient for practical purposes. however, the wide number and variety of methods available for thyroid evaluation offers to pediatricians a choice sometimes difficult. the aim of this review is to summarize the meaning and accuracy of current methods and their usefulness in diagnosis and study of thy ...	1978	213848
a rapid purification of t4 polynucleotide kinase using blue dextran-sepharose chromatography.	a rapid batch procedure is described for purification of t4 polynucleotide kinase (atp:5'-dephosphopolynucleotide 5'-phosphotransferase, ec 2.7.1.78) to near homogeneity using blue dextran-sepharose chromatography. the enzyme preparation is sufficiently free of contaminating endonuclease and alkaline phosphatase activities to be suitable for radioactively labeling nucleic acids in vitro. kinetic measurements indicate that the chromophore of blue dextran, cibacron blue f3ga, inhibits the activity ...	1978	214125
inhibition of thyroidal cyclic amp-dependent protein kinase by thyroid hormone.	bovine thyroid cyclic amp-dependent protein kinase was purified by deae-sephadex and sephadex g-200 chromatography. this preparation showed a 240-fold increase in specific activity over the initial 20,000 x g supernatant with histone as substrate and 1 micronm cyclic amp in the assay mixture. in the presence of 2.5 x 10(-5)m l-triiodothyronine (t3), protein kinase activity was significantly reduced; 50% inhibition was achieved at 1 x 10(-4) m. tests of diverse thyroid hormone analogs showed that ...	1978	214293
perturbations in simian virus 40 dna synthesis by ultraviolet light.	perturbations of simian virus 40 (sv40) dna replication by ultraviolet (uv) light during the lytic cycle in permissive monkey cv-1 cells resemble those seen in host cell dna replication. formation of form i dna molecules (i.e. completion of sv40 dna synthesis) was more sensitive to uv irradiation than synthesis of replicative intermediates or form ii molecules, consistent with inhibition of dna chain elongation. the observed amounts of [3h]thymidine incorporated in uv-irradiated molecules could ...	1978	214706
radiation-induced damage in t4 bacteriophage: the effect of superoxide radicals and molecular oxygen.		1978	214811
triiodothyronine (t3) concentration and therapy in autistic children.	the clinical and biochemical status of thyroid function of patients with an autistic syndrome was investigated. the study consisted of 13 patients between the ages of 7 and 21 years. there was no clinical evidence for hypothyroidism in any patient, and t3, t4, and tsh concentrations were within the normal range. two patients who had retarded bone ages were treated with triiodothyronine for 6 months. hyperthyroidism developed when t3 levels exceeded physiologic concentrations in these patients. t ...	1978	215586
recombinational repair of alkylation lesions in phage t4. ii. ethyl methanesulfonate.	treatment of bacteriophage t4 by ethyl methanesulfonate (ems) caused more than a doubling in recombination between two rii markers. the functions of genes 47, 46, 32, 30, uvsx and y are known to be required for genetic recombination, and mutants defective in these genes were found to be more sensitive to inactivation by ems than wild-type phage. this suggests that a recombinational pathway involving the products of these genes may be employed in repairing ems induced lethal lesions. genes 45 and ...	1978	215891
[therapeutical test of euthyral in thirty one cases of hypothyroidism (author's transl)].	euthyral was prescribed in 31 cases of hypothyroidism. plasma levels of t3, t4 and tsh were measured. plasma tsh returned to normal values in all cases when daily doses of euthyral exceeded 3/4 of a tablet. plasma t4 returned to normal values in all cases when daily doses of euthyral exceeded 3/4 of a tablet. plasma t4 returned to normal values in all cases when daily doses of euthyral exceeded half a tablet. plasma t3 returned to normal values in all cases but was above normal values in 30 per ...	1978	216112