Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [an evaluation of a t4 ria kit using solid phase method--spac t4 ria (author's transl)]. | 1978 | 725128 | |
| t4 toxicosis. | we have described a 25-year-old man with hyperthyroidism and normal levels of serum t3 and reverse t3, but with abnormalities in serum t4i, t3u, fti, ft4, and t3 suppression and trh stimulation tests consistent with the diagnosis of t4 toxicosis. serum total iodine was elevated several-fold, and the observed response to iodine depletion and loading suggested that the t4 toxicosis in this patient probably resulted from an increased iodine load, with production of t4 preferentially to t3. there ap ... | 1978 | 725642 |
| head morphogenesis of bacteriophage t4. i. isolation and characterization of gene 40 mutants. | 1978 | 726257 | |
| head morphogenesis of bacteriophage t4. ii. the role of gene 40 in initiating prehead assembly. | 1978 | 726259 | |
| head morphogenesis of bacteriophage t4. iii. the role of gene 20 in dna packaging. | 1978 | 726265 | |
| morphological development of the epithelium of the embryonic chick intestine in culture: influence of thyroxine and hydrocortisone. | a culture system with defined medium has been utilized to elucidate the role of thyroxine (t4) and hydrocortisone (hc) in growth and differentiation of the epithelium of embryonic chick duodenum during the third week of development. duodenal explants maintained a basically normal morphology for 48 to 72 hours, but during subsequent culture, muscle and mesenchyme deteriorated, previllous ridges shrank, the epithelial surface became pitted, and the tissue lost weight. these degenerative processes ... | 1978 | 727155 |
| t4 rna ligase joins 2'-deoxyribonucleoside 3',5'-bisphosphates to oligodeoxyribonucleotides. | t4 rna ligase catalyzes the atp-dependent addition of a single 2'-deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of an oligodeoxyribonucleotide. the bisphosphate is joined to the deoxyoligomer by a 3' leads to 5' phosphodiester bond and the product, which is terminated by a 3'-phosphate, is one nucleotide longer than the substrate. bisphosphates of dado, dcyd, dguo, dthd, and durd are donors and oligodeoxyribonucleotides with da, dc, dg, dt, or du 3' termini act as acceptors. the pref ... | 1978 | 728390 |
| [development of the response of rabbit fetal tissues to thyroxine]. | development of rabbit fetal tissues sensitivity to thyroxin was studied on the basis of changes in alpha-glycerophosphate oxidase activity in the liver mitochondria of pregnant rabbits and their fetuses to which thyroxin was administered at different stages of pregnancy. t4 administration up to the 23rd day of gestation caused an increase in the enzyme activity in the maternal liver mitochondria only, whereas in the fetus it was at the control level. from the 24th day of pregnancy an increase of ... | 1978 | 728617 |
| [early detection of congenital hypothyroidism--determination of total thyroxine using the ria t4 spot-ostion method (author's transl)]. | 1978 | 728936 | |
| endogenous serum tsh levels and metastatic survey scans in thyroid cancer patients using triiodothyronine withdrawal. | using a triiodothyronine withdrawal method to produce a hypothyroid state in 38 patients with prior thyroidectomies for differentiated thyroid cancer, 56 h-tsh and t4 levels were measured prior to metastatic survey scans performed with 131i. all the t4 levels were below normal and 55 of the 56 h-tsh levels were elevated. no qualitative differences could be observed in the eight patients who had scans performed after bovine tsh injections and later after triiodothyronine withdrawal. triiodothyron ... | 1978 | 729314 |
| cholinergic nerve development of fetal lung in vitro. | the development of the cholinergic or parasympathetic nervous system was studied in embryonic lung dissociated from the central nervous system and placed in organ culture. in vitro development was similar to that seen in vivo. this study demonstrated that migration of neuroblasts from the central nervous system to the trachea takes place before day 12. neuroblasts differentiate to small immature ganglia and then to larger more mature ganglia that give off nerve fibers to the submucosa and the ep ... | 1978 | 731366 |
| [the effects of premedication on thyroid hormone t3, t4 and r.s.u. in man (author's transl)]. | 1978 | 731849 | |
| recombinational repair of alkylation lesions in phage t4. i. n-methyl-n'-nitro-n-nitrosoguanidine. | treatment of phage t4-host adsorption complexes by mnng increased recombination between two rii markers by about three-fold. temperature sensitive mutants defective in genes 32, 46 and 47, which cause reductions in recombination at semirestrictive temperature, proved to be substantially more sensitive to mnng at such temperatures than wild-type phage. in addition, the recombination defective mutants xm(uvsx) and y10(y) were sensitive to mnng than wild-type, whereas mutants defective in genes 45 ... | 1978 | 732807 |
| [phagocytic lymphocyte-like elements (plle) and irradiation in vitro]. | the peripheral blood of rats contains lymphocyte-like phagocytes after intracardial injection of india ink. in vitro irradiation of whole blood (1000 r - 60co), causes marked reduction in whole lymphocytes but does not modify the number and morphological integrity of the phagocytes, which can therefore be held to be comparatively radioresistant: as such, they could belong either to the mononucleate phagocyte system or to the lymphatic system (t4 lymphocytes). since t-lymphocytes are not capable ... | 1978 | 733059 |
| [the neonatal detection of congenital hypothyroidism (author's transl)]. | congenital hypothyroidism appears to be the commonest infantile endocrine disease (1/3000 to 1/5000 newborn). previous experience has shown that the clinical diagnosis during the neonatal period is often difficult and that the long term result is dependent upon the rapidity with which hormone treatment is begun. a rational approach to these data and the possibility of miniaturisation of methods for the radio-immunological estimation of t4 and tsh led to the possibility of neonatal detection on a ... | 1978 | 733488 |
| [determination of thyroxine in serum by gammacoat t4 (author's transl)]. | 1978 | 734168 | |
| studies on the synthesis of polynucleotides--synthesis of a dodecaribonucleoside undecaphosphate and a hexadecaribonucleoside pentadecaphosphate. | this paper describes the synthesis of oligoribonucleotides by t4 rna ligase and their identification. the products are a dodecaribonucleotide cpgpgpa32pcpupcpgpupcpcpa and a hexadecaribonucleotide apupupc32pcpupcpgpupcpcpa, representing the 3'-end nucleotide sequences of the yeast alanine transfer rna. particular emphasis was placed on the conditions for the joining reaction, using oligoribonucleotides of defined sequence as the substrates. cpgpgpa32pcpupcpgpupcpcpa was synthesized with 32pcpupc ... | 1978 | 734440 |
| molecular weight determination by scanning transmission electron microscopy. | a scanning transmission electron microscope is employed to determine the mass of biological macromolecules. elastically scattered electrons are collected by an annular detector that is capable of counting single electrons. off-line processing of these dark field micrographs stored on a magnetic tape is accomplished by a mini-computer. it allows the number of electrons scattered by spherical or filamentous proteins to be evaluated. the calibration factor relating the number of scattered electrons ... | 1978 | 734784 |
| [plasma levels of 3,3', 5'-t3 (reverse-t3) under various functional thyroid conditions]. | reverse t3 (r-t3) was measured in unextracted human plasma under different clinical conditions. the mean normal concentration was 0.20 ng/ml. in thyreotoxic patients r-t3 was elevated in the majority of the cases, however, it was normal in three cases of t3-toxicoses. thyrectomized patients during different hormon substitution showed r-t3 levels corresponding to the t4 concentrations measured. patients with severe chronic illnesses and low t3 showed normal to considerably elevated r-t3 levels. a ... | 1978 | 735616 |
| role of thyroid gland in oxygen toxicity. | the effects of hyperoxia at ambient pressure on thyroid function and thyroid hormone metabolism have been assessed. thyroidal activity was depressed in mice and rats by exposure to hyperoxia, due at least in part to a decrease in the rate of secretion of pituitary thyrotropin. the effects of hyperoxia on the peripheral deiodination of thyroxine (t4) were dependent on the concentration of oxygen employed and/or the duration of exposure; exposure to 40--80% oxygen for 96 h resulted in decreases in ... | 1978 | 736122 |
| [low triiodothyronine syndrome in non thyroidal diseases. distribution and serum tsh concentration studies (author's transl)]. | thyroid explorations were made in 57 patients complaining of serious illnesses in order to identify "low t3 syndrome". all these patients were clinically euthyroid as assessed by normal values of t4 concentration, rt3u ratio and ft4i. however, all the patients included in this study had significantly low serum t3 (42 +/- 29 ng/100 ml) and ft3i (0,44 +/- 0,30). low t3 syndrome was particularly frequently seen in patients with cancer (8/10), hepatic cirrhosis (5/6), renal failure (6/7), old age (5 ... | 1978 | 736516 |
| autogenous translational repression of bacteriophage t4 gene 32 expression in vitro. | 1978 | 739544 | |
| [measurement of reverse triiodothyronine (rt3) by direct radioimmunoassay: serum rt3 distribution and correlation with triiodothyronine (t3), thyroxine (t4) and free thyroxine index (fti) in various thyroid economy (author's transl)]. | 1978 | 739674 | |
| evidence for a near uv-induced photoproduct of 5-hydroxymethylcytosine in bacteriophage t4 that can be recognized by endonuclease v. | non-photoreactivable endonuclease v-sensitive sites have been detected in the dna of wild type bacteriophage t4 irradiated with near uv light (320 nm). such sites were not detected in the dna of (a) wild type t4 irradiated with far uv (254 nm) or (b) in t4 mutants in which non-glucosylated 5-hydroxy-methylcytosine (5hmc) or cytosine replaces glucosylated 5hmc normally present in t4, irradiated with 320 nm or 254 nm light. although the non-photoreactivable sites accounted for approximately 50% of ... | 1978 | 739976 |
| [water and potassium metabolism in functional disturbances of the thyroid gland (author's transl)]. | total body water, extracellular water (bromide space), plasma volume and total body potassium were evaluated in hypothyroid (n = 24) and hyperthyroid (n = 18) patients and in controls (n = 10). whereas the relative extracellular water was the same in all groups investigated, the relative intracellular water was found to be reduced in hyperthyroidism and increased in hypothyroidism (p less than 0.05). as to plasma volume there were no significant differences. a negative correlation between total ... | 1978 | 740541 |
| [comparative studies of commercially available t4-ria-kits (author's transl)]. | the investigation of 4 commercially available t4-ria-kits with 350 patient sera have shown that: the separation of hypo-, eu- and hyperthyroid values is easier with kit a) and d) than with b) and c), though all kits give acceptable values under routine conditions. for the determination of the "normal range" not less than 100 patient sera are necessary. the time needed for 100 determinations is 2 to 3 hr. a general recommendation for one of the tested t4-ria-kits cannot be made since the choice d ... | 1978 | 740544 |
| [comparative studies of commercially available t4-ria-kits (author's transl)]. | the investigation of the tested commercially available t4-ria-kits have shown that: the separation of hypo-, eu- and hyperthyroid values is better with kits a) and f) than with b),c),d) and e), though all kits give acceptable values under routine operating conditions, kit b) takes less time and work than the other tested kits, depending on the kit used, the time needed for 100 determinations varies between 1,5 and 4 hr. a general recommendation for one of the tested t4-ria-kits cannot be made si ... | 1978 | 740545 |
| [relation between the plasma levels of t3 and t4 in thyroid hyperfunction. t3 and t4 hyperthyroidism]. | 1978 | 741005 | |
| management of urinary bladder cancer. | a series of 231 patients, 198 males and 33 females, suffering from primary urinary bladder cancer and treated by various combinations of surgery, radiotherapy and cancer chemotherapy, was categorized into 4 treatment groups, analysed and compared with those recorded in the literature. patients in stages t1 and t2, if treated adequately, have an excellent and good prognosis respectively, while those in stages t3 and t4, unfortunately the majority, have a prognosis that is decidedly disappointing. ... | 1978 | 741295 |
| treatment of bladder cancer at the rotterdam radiotherapy institute (r.r.t.i.) with special reference to bladder radium implantation and preoperative radiotherapy followed by cystectomy. | between 1950 and 1972, a total of 2,031 cases of bladder cancer were treated in the r.r.t.i. the policy differed according to size and stage of the disease as well as to the general condition of the patient. best results were achieved by radium implantation of solitary lesions less than 5 cms in diameter. preoperative radiotherapy followed by cystectomy was the treatment of choice for cases of t3 category. the remaining t1 and t2 cases were treated surgically, while t4 cases were managed with ex ... | 1978 | 741536 |
| substrate specificity of gene 49-controlled deoxyribonuclease of bacteriophage t4: special reference to dna packaging. | 1978 | 741651 | |
| metabolic responses to adrenaline and muscle glycogen content in dogs treated with thyroxine. | lipolytic, hyperglycaemic and lactacidaemic responses to 1h adrenaline infusion (0.1 microgram/kg/min) were compared in resting dogs before (control) and after prolonged thyroxine (t4) treatment. besides, the effect of 2-week thyroxine administration on muscle glycogen content, and its changes following adrenaline infusion were examined. prolonged t4-treatment of dogs resulted in considerable alterations of the metabolic actions of adrenaline. a marked difference between the control and t4-treat ... | 1978 | 742367 |
| relative increase of serum reverse t3 in patients with hypothyroidism. | in 17 hypothyroid patients serum t3: rt3 ratio was 7.5 /+- 1.1 which was significantly lower than in control subjects (12.2 /+- 0.6; p less than 0.001). the data suggest that in hypothyroidism the organism might shift conversion of t4 from biologically active t3 to biologically inactive rt3 which may not be a defense mechanism of the body, as it was found in chronic systemic illness. | 1978 | 742837 |
| [reverse t3 (rt3), t3 and t4 in chronic aggressive hepatitis, cirrhosis and hepatic neoplasms]. | 1978 | 743477 | |
| preparation, purification, and stability of high specific activity 125i-labeled thyronines. | a modified chloramine-t method is described for the preparation of several radioiodothyronines by an exchange reaction, which results in low specific activity preparations, or by an addition reaction, which yields radioiodothyronines with specific activities up to 7 mci/microgram. purification by paper chromatography is shown to be more convenient than by lh-20 chromatography and provides better resolution among the various thyronines. radioiodothyronines with only a single iodine atom in the ou ... | 1978 | 743943 |
| excess production of free alpha subunits by mouse pituitary thyrotropic tumor cells in vitro. | primary cultures from three serially transplanted mouse pituitary thyrotropic tumors, furth 97a, 97b, and 97c, and one newly generated tumor, nih 101a, were shown to secrete free alpha subunits and a beta subunit (tsh-beta) in addition to intact tsh. the three glycopeptides were measured in specific, heterologous radioimmunoassays. basal secretion of alpha subunit was found to be in excess in all four thyrotropic tumor cultures, ranging from 5.7- to 8.9-fold higher than that of intact tsh and 30 ... | 1978 | 743972 |
| serum somatomedin stimulation in thyroxine-treated hypophysectomized rats. | somatomedin activity in rat serum was measured by sulfate incorporation into embryonic chick cartilage. hypophysectomized male rats were treated daily for 21 days with 20 microgram/kg l-thyroxine (t4) and/or 25 microgram bovine (b) gh. serum from rats given t4 alone or with bgh had somatomedin potencies of 0.88 and 0.83, respectively, comparable to normal serum (1.00) and significantly greater than serum from bgh-treated hypophysectomized rats (0.62). hypophysectomized rats had no measurable som ... | 1978 | 743982 |
| conversion of l-thyroxine to triiodothyronine in rat kidney homogenate. | rat kidney homogenates, in phosphate-edta buffer, consistently catalyzed the formation of t3 from added l-thyroxine (t4). the formation of t3 was assessed by both paper chromatography and ria of t3. conversion of t4 to t3 appeared to be enzymatic, showing ph and temperature optima (ph 7.0 and 37 c, respectively) and tissue and time dependence. formation of t3 was unaffected by azide, cyanide, or catalase, nor was it dependent upon oxygen; indeed, under anaerobic conditions conversion of t4 to t3 ... | 1978 | 743983 |
| non-parallel variations in the preferential secretion of 3,5,3'-triiodothyronine (t3) and 3,3',5'-triiodothyronine (rt3) from dog thyroid. | rt3, t3, and t4 were measured in effluents from perfused dog thyroids and in hydrolysates of the thyroids. rt3 was determined by a newly developed sensitive radioimmunoassay (sensitivity approximately 0.1 pg rt3/tube). the rt3 and t3 contents of thyroid effluent were always much higher than would be expected from the relative amounts of t4, t3, and rt3 in thyroid hydrolysate. in four two-sided thyroid perfusions the t4/rt3 ration (wt/wt) in thyroid hydrolysates was 59.3 +/- 26.3 (mean +/- sd) an ... | 1978 | 743992 |
| monodeiodination of 3,5,3'-triiodothyronine and 3,3',5'-triiodothyronine to 3,3'-diiodothyronine in vitro. | to study conversion of 3,5,3'-triiodothyroinine (t3) and 3,3',5'-triiodothyronine (rt3) to 3,3'-diiodothyronine (t2) in vitro, t3 or rt3 was incubated at ph 7.35 with homogenates of several rat tissues (liver, kidney, muscle, heart, ling, spleen, intestines, and brain) for 15 min at 37 c. the t2 generated during incubation was measured in an ethanol extract of the incubation mixture by a specific ria of t2; t4, t3, and rt3 cross-reacted in the t2 ria only to an extent of 0.006, 0.2, and 0.04%, r ... | 1978 | 744010 |
| decreased thyroid function and high plasma prolactin levels in rats of the buffalo strain. | rats of the inbred buffalo strain have previously been reported to be susceptible to thyroiditis, as defined by histology. we have studied the endocrinology of the pituitary-thyroid axis of this strain by making direct measurements of the plasma concentrations of tsh and t4 in untreated, adult buffalo rats of both sexes. plasma prl levels were also measured. all hormone determinations were by ria. in addition, relative thyroid weights were noted and, in many cases, preliminary assessment of thy ... | 1978 | 744034 |
| thyroid hormone analogs and fetal goiter. | transfer of iodothyronine across the placenta in most species occurs only with difficulty. recently, biologically active, nonhalogenated thyroid hormone analogs have been synthesized with properties which might favor placental transfer. to test this possibility, we compared the doses of t4, t3, and thyroid hormone analogs necessary to prevent propylthiouracil-induced goiter formation in rat fetuses. t4 and t3 prevented fetal goiter, but in doses that caused maternal hyperthyroidism; in contrast, ... | 1978 | 744045 |
| serum triiodothyronine in undernourished rats: dependence on dietary composition rather than total calorie or protein intake. | serum triiodothyronine (t3) in rats did not change if food intake was restricted without altering the composition of the diet. feeding a hypocaloric low-protein high-carbohydrate diet caused increases in serum t3, while feeding a hypocaloric high-protein low-carbohydrate diet caused decreases in serum t3. these changes were not related to reductions in intake of either calories or protein, and concomitant changes in serum thyroxine (t4) were not observed. reduction in the dietary content of vali ... | 1978 | 744060 |
| changes in the fetal thyroid axis after induction of premature parturition by low dose continuous intravascular cortisol infusion to the fetal sheep at 130 days of gestation. | the effect of cortisol infusion on plasma t4 and t3 concentrations has been investigated in the fetal lamb at 130-134 days of gestation. infusion of cortisol at rates equivalent to one-third the quantity produced by the fetus at the time of parturition results in a significant fall in fetal plasma t4 concentration and a significant rise in fetal plasma t3 concentration. delay in the initiation of labor as a result of progesterone administration to the ewe did not prevent the changes in the fetal ... | 1978 | 744069 |
| subcellular distribution of thyroxine 5'-deiodinase in the rat kidney: a plasma membrane location. | the enzymatic activity in rat kidney homogenates mediating the conversion of t4 to t2 has been previously shown to be particulate. the subcellular organelle responsible for t4 5'-deiodination, however, has not been identified. enzymatic activity was assessed with outer ring 125i-labeled l-t4 as the substrate and the iodothyronines were separated by descending paper chromatography. comparison of the distribution of t4 5'-deiodinase activity with that of established enzyme markers of subcellular o ... | 1978 | 744079 |
| metabolic clearance of endogenous and radioiodinated thyroglobulin in rats. | the basal concentration and metabolic clearance of radiolabeled and endogenous thyroglobulin (tg) were measured in normal male rats. serum tg concentrations (by ria) in sprague-dawley adult males ranged from 70-680 ng/ml (mean +/- sd, 218 +/- 177). in male fisher rats, basal serum tg concentrations were 32-263 ng/ml. the fractional disappearance rate of endogenously radioiodinated rat tg and of endogenous rat tg after thyroidectomy were both about 8%/h. the mcr of endogenously labeled tg was 2.4 ... | 1978 | 744090 |
| maternal, fetal, and amniotic fluid transport of thyroxine, triiodothyronine, and iodide in sheep: a kinetic model. | a mathematical model of iodine kinetics in maternal and fetal sheep has been developed by combining separate iodide, t3, and t4 subsystems. the individual subsystem models were developed from literature studies of maternal-fetal exchange under thyroid-blocked and unblocked conditions. rates of exchange, concentrations, and spaces of distribution were calculated by the saam computer program. the models for each of the subsystems required exchange compartments within the mother and fetus, exchange ... | 1978 | 744102 |
| comparison of pituitary-thyroid maturation in the fetuses of rats fed an iodine-deficient or normal diet. | from the earliest detectable development of fetal pituitary-thyroid function (day 18-19 of gestation) through the first postnatal day, there was a higher degree of stimulation of the pituitary-thyroid axis in the fetuses of rats fed a low iodine diet (lid) than in those of rats fed a high iodine diet (hid). significant differences between the two groups were consistently observed in relative thyroid size, plasma tsh, 4-h thyroid radioiodine uptake, and the labeled iodoamino acid composition of t ... | 1978 | 744106 |
| inhibition by adenosine of thyroidal t4 release in vitro. | adenosine, like catecholamines, inhibits the thyroidal t4 release in vitro, when stimulated by tsh,n,o'-dibutyryl cyclic amp [(bu) 2camp], and phosphodiesterase inhibitors. unlike catecholamines, the adenosine-induced inhibition is independent of adrenergic receptors. it is postulated that tsh stimulates thyroidal t4 release through a camp activated, adenosine-sensitive, protein kinase. | 1978 | 744109 |
| metabolism of thyroid hormones by rat thyroid tissue in vitro. | rat thyroid lobes or hemilobes have been incubated in krebs-ringer phosphate buffer containing labeled t4 and/or t3, and the products were separated by paper chromatography. labeled t4 was actively degraded; about half of the t4 metabolized was recovered as t3. labeled t3 was also metabolized, but less rapidly than t4. other than t3 produced from t4, the major products from both hormones were inorganic iodide and iodoprotein; the latter was presumably a secondary product of iodide organification ... | 1978 | 744119 |
| quantitative electron microscopic studies on exocytosis and endocytosis in the thyroid follicle cell. | the effect of tsh (0.5 u), given iv to t4-treated rats, on the distribution of membranes in the apical part of the thyroid follicle cell was investigated by electron microscopic morphometry. in unstimulated cells, the membrane surface area of exocytotic vesicles (ev) was slightly larger than that of the apical plasma membrane; the latter had an estimated surface area of 180-190 micron2/follicle cell. three phases in the action of tsh could be discerned. a first phase (up to 5 min) was dominated ... | 1978 | 744123 |
| selective inhibition of the secretion of triiodothyronines from the perfused canine thyroid by propylthiouracil. | in order to assess a possible influence on the relative proportions of t4, t3, and rt3 in thyroid effluent, the thyroid-blocking agents, propylthiouracil (ptu) and methylmercaptoimidazol (mmi), were administered to perfused canine thyroids. the dog has two distinct thyroid lobes which were both perfused independently, one acting as a control for the other. t4, t3, and rt3 in thyroid effluents and thyroid hydrolysates were measured radioimmunologically. during control perfusion the t4:t3 and t4:r ... | 1978 | 744124 |
| effect of parturition on serum iodothyronine concentrations in fetal sheep. | t4, t3, and rt3 were determined by ria on daily serum samples collected from five catheterized fetal sheep during the 2-week period before spontaneous vaginal delivery (ptd). samples were also available from three newborn lambs during the first 4 h of life. t3 concentrations began to increase 4-6 days before spontaneous vaginal delivery, from a stable geometric mean concentration of 30 ng/dl to a mean concentration of 131 ng/dl on the day of delivery (day 0). t4 concentrations decreased in four ... | 1978 | 744158 |
| site specificity and variability in the mutator and antimutator effects of phage t4 gene 43 mutants. | spontaneous, 2-aminopurine- and 5-bromouracil-induced mutations at six rii nonsense codons were studied in phage t4 strains possessing wild-type and mutant gene 43 alleles. the mutation pathways studied included interconversions and reversions of nonsense codons. the tscb87 allele, which specifies an antimutator dna polymerase, reduced base-analogue-induced mutation frequencies along all pathways. however, gc base pairs were less affected than at base pairs. the frequency of spontaneous uaa lead ... | 1978 | 744472 |
| pituitary-thyroid axis during short term, mild and severe, iodine depletion in the rat. | to investigate the most early events occurring during the adaptation of the pituitary-thyroid axis to iodine depletion, two rat populations were submitted to 4 week low iodine regimens of different severity. the variations of the following parameters were studied: pituitary thyrotropin (tsh) concentration, serum thyroxine (t4), triiodothyronine (t3) and tsh, thyroid weight and thyroid iodine concentration. in the first population, mildly iodine depleted, serum and pituitary tsh remained unchange ... | 1978 | 744571 |
| peripheral metabolism of t4, t3, reverse t3, 3',5'- diiodothyronine and 3,3' diiodothyronine in liver cirrhosis. | 1978 | 744581 | |
| isolation and characterization of conditional lethal mutations in the mot gene of bacteriophage t4. | 1978 | 745239 | |
| hypothyroidism in children with cystinosis. | eight children with cystinosis (3 with renal transplants, 2 on maintenance haemodialysis, 2 with chronic renal failure, and one with normal renal function) were studied for evidence of hypothyroidism, and compared with a control group of children with chronic renal failure due to other causes. abnormal thyroid function was present in all the cystinotic patients: thyroxine (t4) low in 1, free thyroxine index (fti) low in 2, thyroid-stimulating hormone (tsh) raised in 6; all had a supranormal tsh ... | 1978 | 747399 |
| serum concentrations of tsh, t4, and t3 during the first three days of postnatal life. | 1978 | 747672 | |
| euthyroidism via iodide supplementation in hereditary congenital goiter with thyroglobulin deficiency. | we have studied hereditary congenital goiter in an inbred strain of goats. on a normal diet, the goats were hypothyroid and iodide taken up by the gland was released rapidly in the form of iodinated macromolecular material. this resulted in a low thyroidal iodine pool. an extremely low amount of thyroglobulin-related antigens (12 microgram/g tissue; normal, 100 mg/g tissue) was detected in the goitrous gland by ria. addition of 1 mg i-/day to the diet made the goats euthyroid and the serum prote ... | 1978 | 748035 |
| thyroxine 5'-deiodinase activity of rat kidney: observations on activation by thiols and inhibition by propylthiouracil. | enzymatic 5'-deiodination of t4 in various tissues is known to be stimulated by thiols and inhibited by propylthiouracil (ptu). dithiothreitol (dtt) stimulation of rat kidney t4 5'-deiodinase followed saturation kinetics. inhibition of the enzyme by ptu (10 (-5) m) was overcome in a concentration-dependent manner by dtt, methimazole (mmi), and thiourea. pretreatment of catalytically active kidney microsomal preparations with pts caused persistent inhibition of enzyme activity, assayed in the abs ... | 1978 | 748037 |
| [fundamental and clinical evaluation of "seralute total t4 (ria)" kit (author's transl)]. | 1978 | 748652 | |
| electron microscopy of dna molecules isolated from t4 "petite" particles. | 1978 | 749911 | |
| visual, auditory and somatosensory evoked responses in patients with cerebrovascular disorders. | a diagnostic evoked response battery, consisting of visual evoked responses to flashes and to a checkerboard pattern, auditory evoked responses to three different tones and somatosensory evoked responses to left, right and bilateral median nerve stimulation, was administered to 20 patients with strokes, and compared with 20 normal control subjects. recordings were obtained at c3, c4, o1, o2, t3 and t4. evoked responses were found to be of lower amplitudes and lower amplitudes and lower waveform ... | 1978 | 751420 |
| [approach to a differentiated recurrence prevention following struma surgery]. | changes of the pituitary-thyroid axis were studied in 70 patients (45 with euthyroid goiters, 25 with autonomous adenomas) preoperatively; over a 6-week postoperative interval without thyroid-hormone treatment; under t4 therapy increased stepwise and maintained for 1 year; and over a period of 12 weeks after its withdrawal. the postoperative thyrotropic function obviously depends on the preoperative condition and thereby on the quality of the remaining tissue as well as on the extent of the oper ... | 1978 | 752582 |
| results of a randomized trial of prophylactic chemotherapy in t3-t4 breast cancer patients previously treated by radiotherapy. | 1978 | 752850 | |
| [circadian variations of t3 and t4 in euthyroid subjects]. | 1978 | 753273 | |
| adoptive immunotherapy and radiotherapy in the treatment of urinary bladder cancer. | thirty-one patients with invasive transitional cell carcinoma of the urinary bladder were treated either by infusion of tumour immune pig mesenteric lymph node cells into the tumour blood supply, followed 6 weeks later by 5500 rad, or by radiotherapy alone. the patients were paired prior to treatment, using 4 criteria: age (nearest decade), sex, clinical stage (t3 or t4) and histological grade (average or high). the first patient in each pair then received combined treatment and the second radio ... | 1978 | 753485 |
| blood-c.s.f. barriers dysfunction in the chronic organic brain syndrome; a r.i.a. study. | c.s.f. samples of 35 patients, who suffered from verified chronic, non-tumorous organic brain syndrome, were radioimmunoassayed for t4 and t.s.h., and were compared to c.s.f.-r.i.a. samples from a control group of patients who underwent myelography because of lumbar disc. in addition t4 and t.s.h. plasma levels were evaluated in the o.b.s. patients. c.s.f. t4 and t.s.h. levels were significantly higher in 65% of the o.b.s. group of patients than those of the control group. the average determinat ... | 1978 | 754277 |
| [further data on the dependence of the agglutination titre of t4 streptococci on the haptoglobin type (hp 2-2 and hp 2-1)]. | anti-streptococcal t4 titres in human sera tend to be higher in normal sera of the hp type hp 2-2 than in normal sera of hp 2-1 subjects. if the sera are heated above a temperature of 50 degrees c the difference disappeared and the titres are reduced. when heating sera to 63 degrees for 10 min no agglutination activity for t4 streptococci can be observed. | 1978 | 754426 |
| cord blood reverse t3 in normal, premature, euthyroid low t4, and hypothyroid newborns. | 1978 | 755844 | |
| endemic goitre and iodine metabolism in schoolchildren from the daunia uplands (province of foggia, italy). | a study of endemic goitre was carried out in the western part of the province of foggia (daunia's pre-appennines). about 50% of a total of 2719 children aged 6-15 years examined in the agricultural and hill towns of accadia, bovino, candela, deliceto and sant'agata di puglia (500 to 800 m above sea level), presented a thyroid enlargement (usually of grade 1). urinary creatinine values were normal, while urinary iodine was low: 37.7 +/- 28.39 mug/g creatinine (mean +/- sd for 319 subjects). serum ... | 1978 | 755847 |
| postnatal development of pituitary-thyroid function in male and female rats: comparison of plasma and thyroid t3 and t4 concentration. | plasma t4, t3, and tsh, and total and 131l-labeled t4 and t3 in thyroid digests were determined in male and female rats from birth to 30--45 days postnatally. mothers and offspring were fed a high-iodine purina diet throughout. in both sexes, plasma t4 was less than 1 microgram/100ml at birth but rose linearly to a peak of 6--7 micrograms/100 ml at day 15. plasma t3 remained low at 30--40 ng/100 ml until day 10, then rose steeply to 80--100 ng/100 ml at day 20--30. plasma tsh had a bimodal distr ... | 1978 | 756880 |
| [radioimmunological determination of circulating thyroid hormones]. | in our study of the thyroid function, the methodological aspects and clinical significance of tsh, t4, t3 and their respective free fractions (ft4 and ft3), are examined. the ria of tsh is the best for the diagnosis of hypothyroidism and also for the evaluation of the effect of thyroid hormone substitution therapy. this, united with t4 and t3 assay, allows a total evaluation of the thyroid state. the new method, recently introduced, for direct assay of free fractions, using a chromatographic sep ... | 1978 | 757627 |
| the effect of perinatal factors on cord thyroxine concentration. | cord blood thyroxine (t4) concentrations were measured in 4,068 infants from 28 wk gestation to term. each chart was reviewed for the following factors: delivery by cesarean section, prolonged rupture of membranes, neonatal asphyxia, meconium-stained amniotic fluid, maternal diabetes mellitus and twinning. each neonate was evaluated for the idiopathic respiratory distress syndrome, and low (sga) or high (lga) birthweight for gestational age. within each gestational age group, the mean cord t4 va ... | 1978 | 551930 |
| combined chemotherapy-radiotherapy approach in locally advanced (t3b-t4) breast cancer. | combined treatment modality was applied in 110 consecutive women with primary inoperable (t3b-t4) breast cancer. treatment was started with four cycles of adriamycin plus vincristine (av). this was followed in responders by high-energy radiotherapy (rt). at the end of combined therapy, patients in complete remission (cr) were randomized to either no further treatment or six more cycles of chemotherapy. av induced objective response in 89% of patients (complete 15.5%, partial 54.5%, improvement 1 ... | 1978 | 373908 |
| [selective binding of oligoribonucleotides by t7 phage induced rna-polymerase]. | it was shown previously that e. coli rna-polymerase being incubated with the random oligonucleotide mixtures of definite length binds certain oligoribonucleotides with the length greater than or equal to 5 nucleotides. the data presented demonstrate that t7 phage induced rna-polymerase (t7 rna-polymerase) also binds selectively oligoribonucleotides beginning from pentaribonucleotides. from the random mixtures of penta-, hexa-, hepta-, octa-, nona- and decaribonucleotides the hepta- and octaribon ... | 1978 | 370554 |
| mccune-albright syndrome in a male child: a clinical and endocrinologic enigma. | a 6 5/12-year-old boy with polyostotic fibrous dysplasia, café-au-lait pigmentation of the skin, and precocious pubertal development was studied for two years. parathormone, calcium, phosphorus, testosterone, cortisol, and growth hormone levels were within normal limits. urinary 17-ketosteroids, 17-ketogenic steroids, and estrogens were at the upper limits of normal. after gnrh stimulation, there was only a very slight increase in lh and no increase in fsh. there was no increase in tsh after trh ... | 1978 | 340627 |
| relationship between haptoglobin and streptococcus pyogenes t4 antigens. | 1978 | 340961 | |
| [specific cleavage of glycosylyl denatured dna of t2 and t4 phages adjacent to the oligothymidilic or oligoadenylic sequences]. | a method of complementarily directed alkylation with following elimination of the alkylated bases and with dna cleavage at apurinic sites was used for specific fragmentation of glucosylyl dna of t2 and t4 phages. it was shown that denatured glucosylyl t2 and t4 dna's are modified by alkylating derivatives of hexaadenylate and heptauridylilate. the extent of alkylation reached the maximum and then stopped. the extent of elimination and chain cleavage corresponded to that of alkylation. treatment ... | 1978 | 368604 |
| phage t4 endonuclease v stimulates dna repair replication in isolated nuclei from ultraviolet-irradiated human cells, including xeroderma pigmentosum fibroblasts. | the repair mode of dna replication has been demonstrated in isolated nuclei from uv-irradiated human cells. nuclei are incubated in a mixture containing [(3)h]thymidine triphosphate and bromodeoxyuridine triphosphate in a 1:5 ratio. the (3)h at the density of parental dna in alkaline cscl density gradients is then a measure of repair. in nuclei prepared from wi38 cells 30 min after irradiation, repair replication is uv dependent and proceeds at approximately the in vivo rate for 5 min. repair re ... | 1978 | 275829 |
| a general method to assess similarity of protein structures, with applications to t4 bacteriophage lysozyme. | a method is proposed that permits the structural similarity between any pair of proteins to be analyzed in a completely general manner. in the proposed procedure, all possible structural segments of a given length from one protein are compared with all possible segments from the other protein. this set of comparisons reveals any structural similarities between the two proteins being compared, and also provides a basis for estimating the probability that a particular degree of structural homology ... | 1978 | 276859 |
| kinetic factors and form determination of the head of bacteriophage t4. | the form of the bacteriophage t4 prehead is described by its icosahedral symmetry, its diameter, and its length. we show how each of these parameters is regulated during prehead formation and ascribe specific form-determining functions to the prehead proteins. the major protein of the head shell can assemble in several different forms. the structure produced in vivo depends on the rate of synthesis of the major protein relative to the rates of synthesis of minor shell proteins and the major core ... | 1978 | 279906 |
| dextrothyroxine treatment of phosphorylase-kinase deficiency glycogenosis in four boys. | four boys, aged 2 years 5 months to 3 years 7 months, with large hepatomegaly due to phosphorylase-kinase deficiency glycogenosis, were given a trial of sodium dextrothyroxine (d-t4) at a mean dose of 0.165 mg/kg/day for an average period of 6 months. phosphorylase-kinase was undetectable in the haemolysates of erythrocytes (3 patients) or in the liver (one patient) before, and still undetectable in the haemolysates of the four patients during treatment, thus pointing to x-linked phosphorylase-k ... | 1978 | 280544 |
| endogenous thymic factors regulating cell proliferation and analysis of their mechanism of action. | endogenous factors inhibiting the proliferation of t-lymphocytes were investigated which may function as modulators of t-lymphocyte production within the thymus. an extract from calf thymus (t4) enriched in lymphocyte chalone arrests rat thymocytes at the g1 leads to s boundary and in the s phase of the cell cycle in short-term cultures. it also inhibits the proliferative response of human peripheral blood lymphocytes to pha-p in a time-dependent manner, as well as the spontaneous proliferation ... | 1978 | 148969 |
| an atp stimulation of t4 dna polymerase mediated via t4 gene 44/62 and 45 proteins. the requirement for atp hydrolysis. | an in vitro replication system reconstituted from six purified t4 bacteriophage proteins, each of which is essential for t4 dna replication in vivo, requires atp. because of the complexity of the complete system, we examine in this report the involvement of atp in two subsystems of the overall dna synthesis reaction. one subsystem consists of the t4 dna polymerase (gene 43 protein) and its "accessory proteins," the gene 44/62 and 45 products. an even simpler subsystem consists of the gene 44/62 ... | 1978 | 149792 |
| analysis of a t4 dna replication protein complex. studies of the dna recognition site for t4 gene 44/62 and 45 protein-catalyzed atp hydrolysis. | 1978 | 149793 | |
| processivity of dna exonucleases. | a homopolymer system has been developed to examine the digestion strategies of dna exonucleases. escherichia coli exonuclease i and lambda-exonuclease, are processive enzymes. however, t7 exonuclease, spleen exonuclease, e. coli exonuclease iii, the 3' leads to 5'-exonuclease of t4 dna polymerase, and both the 3' leads to 5' and the 5' leads to 3' activity of e. coli dna polymerase i dissociate frequently from the substrate during the course of digestion. regions of duplex dna are a dissociation ... | 1978 | 338608 |
| drug therapy reviews: management of hypothyroidism. | the therapeutic management of hypothyroidism caused by deficient thyroid hormone production is discussed. the therapeutic use of the following thyroid agents is reviewed: levothyroxine sodium, thyroid usp, thyroglobulin, liotrix, and liothyronine sodium. myxedema coma, neonatal hypothyroidism, primary hypothyroidism, and secondary and tertiary hypothyroidism are specific hypothyroid states for which drug therapy is discussed. levothyroxine sodium is the preferred agent because of consistent pote ... | 1978 | 341699 |
| streptococcal impetigo and acute glomerulonephritis in children in cairo. | impetigo contagiosa in cairo affected young children of both sexes, the face being the main site. post-impetigo nephritis, confirmed by a low serum c3 level and by urinalysis, occurred in only 11% of cases. streptococcus pyogenes strains were recovered from 84% of the skin lesions. sixteen types were identified according to their t-protein, and most infections were associated with t3/b3264, t13/b3264, t5, t11, t12, t8/25/imp 19 and t14/49; the majority of these types were also recovered from hou ... | 1978 | 341956 |
| [allele specificity of genetic recombination in t4 phage using the indicator crossing study method]. | the diagrams of relative correction ability of eighteen rii mutants of t4 phage were constructed on the basis of two-factor crosses, which were grouped into indicator series. in each series a pair of closely linked compared markers was crossed against indicator ones, the latter being distant enough so as to avoid simultaneous correction with the compared marker. the differences between the frequencies of wild type recombinants in crosses of two compared markers with indicator ones remained const ... | 1978 | 342334 |
| [radiobiological method of studying the structure of the partially diploid area in t4 bacteriophage]. | the mechanism of cross-reactivation after uv-irradiation was examined using different deletion mutants as recipients. a model of the rescue mechanism of partly diploid area was suggested. the method of cross-reactivation in demonstrated to permit an easy determination of the orientation of duplicated genes. | 1978 | 342335 |
| differential effect of hydroxyurea on a ribonucleotide reductase system. | infection of escherichia coli with phage t4 induces a large increase in ribonucleotide reductase activity. we show that hydroxyurea inhibits t4-induced cdp, adp, udp, and gdp reductase activities in vitro. moreover, there are significant differences in the degree of inhibition of each ribonucleotide reductase activity. the reductase activities for cdp and adp are more sensitive to hydroxyurea than those for udp and gdp, particularly at high hydroxyurea molarities. as little as 5 x 10(-4)m hydrox ... | 1978 | 342523 |
| late effect of bacteriophage t4d on the permeability barrier of escherichia coli. | cold centrifugation of lysis-inhibited escherichia coli b infected with wild-type t4d results in extensive lysis beginning around 20 min after infection at 37 degrees c. infection with an e mutant, which fails to make lysozyme, prevents lysis, but does not prevent a marked loss of k+ and mg3+. the t gene product, thought to disrupt the cytoplasmic membrane in natural lysis, is not required for this handling-induced cation loss or lysis. three lines of evidence argue that late protein synthesis i ... | 1978 | 342725 |
| isolation of labeled triiodothyronine from serum using affinity chromatography: application to the extimation of the peripheral t4 to t3 conversion in rats. | a method for the isolation of small quantities of labeled 3,5,3' -triiodothyronine (t3) from serum or thyroid extracts is described. conjugates of rabbit anti-t3 antibody to sepharose 4b are incubated with 0.5 to 1 ml of human or rat serum at ph 8.6 for 1 hr. the tubes are centrifuged and washed with buffer followed by 6 m guanidine to remove nonspecifically bound labeled thyroxine (t4). the fraction of t3 and t4 bound to the sepharose conjugate varies depending on the concentration of serum in ... | 1978 | 342873 |
| in vivo synthesis and properties of uracil-containing dna. | t4 bacteriophage dna containing as much as 30% of its thymine replaced by uracil can be synthesised in escherichia coli deficient in both dutpase and uracil--dna glycosidase. this uracil-containing dna is competent for rna transcription, and can be packaged into phage which are viable, if the host cells are deficient in uracil--dna glycosidase activity. if the host cells are not deficient in this glycosidase activity the infecting phage dna is rapidly attacked, resulting in more than 50% acid-so ... | 1978 | 342978 |
| identification of a host protein necessary for bacteriophage morphogenesis (the groe gene product). | mutations in the groe gene of escherichia coli, which block the correct assembly of the phage lambda head, have been previously described. many groe mutations exert pleiotropic effects, such as inability to propagate phages t4 and t5 and inability to form colonies at 43 degrees. with the help of the ecori and hindiii restrictionenzymes and the appropriate phage vectors, we have constructed two lambda transducing phages, called w3 and h18, that carry the groe+ bacterial gene. upon lysogenization ... | 1978 | 343101 |
| role of the host in virus assembly: cloning of the escherichia coli groe gene and identification of its protein product. | correct assembly of the heads of bacteriophages lambda and t4 requires the function of the groe gene of the escherichia coli host. we have isolated a transducing derivative of lambda, called lambda gt-ec.groe, that carries a functional copy of the groe gene. unlike wild-type lambda, this phage is able to form plaques on hosts with a mutant groe gene. we have isolated an amber mutation in the groe gene carried by the phage, and this has made it possible to identify the groe product as a protein o ... | 1978 | 343102 |
| attachment of tail fibers in bacteriophage t4 assembly. purification, properties, and site of action of the accessory protein coded by gene 63. | 1978 | 344316 | |
| construction and properties of recombinant plasmids containing the rii genes of bacteriophage t4. | the ecori digestion products of phage t4 dna have been examined using a phage dna transformation assay. a 2.6 x 10(6) dalton fragment was found to contain the rii genes. this fragment was purified and then treated with hindiii endonuclease. the cleavage products were ligated to the vector plasmid pbr313 and viable recombinant plasmids recovered. a genetic assay was employed to demonstrate that the recombinants contained t4 dna and to localize on the phage genetic map the ecori and hindiii sites ... | 1978 | 345100 |