Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| respiratory syncytial virus infection suppresses lung cd8+ t-cell effector activity and peripheral cd8+ t-cell memory in the respiratory tract. | respiratory syncytial virus (rsv) is a major cause of morbidity from respiratory infection in infants, young children and the elderly. no effective vaccine against rsv is currently available and studies of the natural history of rsv infection suggest repeated infections with antigenically related virus strains are common throughout an individual's lifetime. we have studied the cd8+ t-cell response during experimental murine rsv infection and found that rsv inhibits the expression of effector act ... | 2002 | 11786907 |
| the enantiomers of carbocyclic 5'-norguanosine: activity towards epstein-barr virus. | (-)-5'-noraristeromycin (1) has shown antiviral activity towards, particularly cytomegalovirus, vaccinia virus and measles while its (+)-enantiomer (2) is effective towards hepatitis b virus. to determine if the antiviral characteristics of 1 and 2 extended to the guanine analogues (3 and 4), these enantiomers were prepared and evaluated against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), cytomegalovirus (cmv), varicella zoster virus (vzv), epstein-barr virus (ebv), human herpes viru ... | 2002 | 11836094 |
| home delivery of palivizumab: outcomes and compliance in regional preterm infants. | palivizumab, a monoclonal antibody against respiratory syncytial virus (rsv), has been shown to be effective in preventing rsv-related hospitalization in preterm infants; however, ensuring infants receive the desired monthly injections remains a challenge. we studied two cohorts of preterm infants and the rate of documented rsv illness in infants receiving palivizumab at home between 1998 and 2000. medical records were reviewed for the number of doses received, hospitalization for rsv illness, a ... | 2002 | 11838265 |
| bronchiolitis: update 2001. | in this review, reports from last year on the following topics are summarized: (1) reviews of bronchiolitis in infants; respiratory syncytial virus (rsv)-associated illness, including possible viral mechanisms of alteration of airway function and results of an epidemiologic study of bronchiolitis-associated mortality. studies evaluating (2) the use of serum eosinophilic cationic protein as a marker for development of subsequent persistent wheezing infants; (3) parental bronchial responsiveness a ... | 2002 | 11845006 |
| rfi-641, a potent respiratory syncytial virus inhibitor. | human respiratory syncytial virus (rsv), a paramyxovirus, is a major cause of acute upper and lower respiratory tract infections in infants, young children, and adults. rfi-641 is a novel anti-rsv agent with potent in vitro and in vivo activity. rfi-641 is active against both rsv type a and b strains. the viral specificity and the large therapeutic window of rfi-641 (>100-fold) indicate that the antiviral activity of the compound is not due to adverse effects on normal cells. the potent in vitro ... | 2002 | 11850270 |
| palivizumab is highly effective in suppressing respiratory syncytial virus in an immunosuppressed animal model. | respiratory syncytial virus (rsv) is widely recognized as a leading cause of pneumonia, with substantial mortality, in bone marrow transplant recipients. we tested the efficacy of a systemic monoclonal antibody (mab) preparation possessing a high titer of anti-rsv neutralizing antibody, palivizumab (synagis) for prophylaxis and therapy of rsv infection in cytoxan (cy) immunosuppressed cotton rats, a model in which the efficacy of a polyclonal anti-rsv product (respigam) has been demonstrated. bo ... | 2002 | 11850705 |
| p60(v-src) and serum control cell shape and apoptosis via distinct pathways in quail neuroretina cells. | we made use of qnr cells transformed by a thermosensitive (tsny68) strain of the rous sarcoma virus (rsv) to compare the effect of p60(v-src) and serum in cultured nerve cells. in this system, both p60(v-src) heat inactivation and serum removal resulted in growth arrest in g1. in both cases, growth arrest was reversible since cell proliferation was rapidly re-induced following respectively p60v-src renaturation or serum re-addition. however, cells did not fully recover their ability to grow in s ... | 2002 | 11850837 |
| epidemiology and clinical presentation of respiratory syncytial virus infection in a rural area of southern mozambique. | respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection (lrti) in infants throughout most of the world, but little is known about rsv infection in africa where lrti are among the leading causes of infant and childhood death. | 2002 | 11840083 |
| caveolin-1 is incorporated into mature respiratory syncytial virus particles during virus assembly on the surface of virus-infected cells. | we have employed immunofluorescence microscopy and transmission electron microscopy to examine the assembly and maturation of respiratory syncytial virus (rsv) in the vero cell line c1008. rsv matures at the apical cell surface in a filamentous form that extends from the plasma membrane. we observed that inclusion bodies containing viral ribonucleoprotein (rnp) cores predominantly appeared immediately below the plasma membrane, from where rsv filaments form during maturation at the cell surface. ... | 2002 | 11842256 |
| alloantigen system l affects the outcome of rous sarcomas. | this study was designed to examine the alloantigen system l effects on rous sarcomas in three b complex genotypes. the parental stock was 50% modified wisconsin line 3 x white leghorn line niu 4 and 50% inbred line 6.15-5. pedigree matings of two b(2)b(5) l(1)l(2) sires to five b(2)b(5) l(1)l(2) dams per sire produced experimental chicks segregating for b and l genotypes. chicks were inoculated with 20 pock-forming units (pfu) of rous sarcoma virus (rsv) at 6 weeks of age. tumors were scored six ... | 2002 | 11856813 |
| effect of lack of interleukin-4, interleukin-12, interleukin-18, or the interferon-gamma receptor on virus replication, cytokine response, and lung pathology during respiratory syncytial virus infection in mice. | rsv is an important cause of bronchiolitis in infants. immunopathology may play a role in rsv-induced bronchiolitis and severe rsv-induced disease has been associated with a th2 type immune response. the aim of the study was to identify cytokine pathways that are crucial in influencing rsv-induced disease. for that purpose we inoculated ifngammar-/-, il-12-/-, il-18-/-, or il-4-/- mice with rsv. we observed that an rsv infection resulted in a predominant th1 cytokine response associated with sli ... | 2002 | 11857536 |
| enhanced immune protection by a liposome-encapsulated recombinant respiratory syncytial virus (rsv) vaccine using immunogenic lipids from deinococcus radiodurans. | the radiation-resistant bacterium, deinococcus radiodurans contains a variety of phospho-, glyco- and phosphoglycolipids, the structures of which appear to be largely unique in nature. we show here that such lipids are immunogenic when administered as liposomes intranasally in mice, as evidenced by the induction of serum antibodies which recognize d. radiodurans lipids but not other lipids by thin layer chromatographic immunostaining. by modifying a liposomal vaccine against respiratory syncytia ... | 2002 | 11858866 |
| tumor-specific transcriptional targeting of suicide gene therapy. | transcriptional targeting of gene expression has been plagued by the weakness of tissue-specific promoters. thus, to increase promoter strength while maintaining tissue specificity, we constructed a recombinant adenovirus containing a binary promoter system with a tumor-specific promoter (cea; carcinoembryonic antigen) driving a transcription transactivator, which then activates a minimal promoter to express a suicide gene (hsv-tk; herpes simplex virus thymidine kinase). this adv/binary-tk induc ... | 2002 | 11859419 |
| construction and characterization of affibody-fc chimeras produced in escherichia coli. | affibody-fc chimeras were constructed by genetic fusion between different affibody affinity proteins with prescribed specificities and an fc fragment derived from human igg. using affibody ligands previously selected for binding to respiratory syncytial virus (rsv) surface protein g and thermus aquaticus (taq) dna polymerase, respectively, affibody-fc fusion proteins showing spontaneous fc fragment-mediated homodimerization via disulfide bridges were produced in escherichia coli and affinity pur ... | 2002 | 11861078 |
| budding of equine infectious anemia virus is insensitive to proteasome inhibitors. | the only retrovirus protein required for the budding of virus-like particles is the gag protein; however, recent studies of rous sarcoma virus (rsv) and human immunodeficiency virus have suggested that modification of gag with ubiquitin (ub) is also required. as a consequence, the release of these viruses is reduced in the presence of proteasome inhibitors, which indirectly reduce the levels of free ub within the cell. here we show that the budding of equine infectious anemia virus (eiav) from i ... | 2002 | 11861830 |
| in vivo interference of rous sarcoma virus budding by cis expression of a ww domain. | for all enveloped viruses, the actual mechanism by which nascent virus particles separate or "pinch off" from the cell surface is largely unknown. in the case of retroviruses, the gag protein drives the budding process, and the virus release step is directed by the late (l) assembly domain within gag. a ppppy motif within the l domain of rous sarcoma virus (rsv) was previously characterized as being critical for the release of virions and shown to interact in vitro with the ww domain of yes-asso ... | 2002 | 11861846 |
| identification of temperature-sensitive mutations in the phosphoprotein of respiratory syncytial virus that are likely involved in its interaction with the nucleoprotein. | the phosphoprotein (p) of human respiratory syncytial virus (rsv) is an essential component of the viral rna polymerase, along with the large polymerase (l), nucleocapsid (n), and m2-1 proteins. by screening a randomly mutagenized p gene cdna library, two independent mutations, one with a substitution of glycine at position 172 by serine (g172s) and the other with a substitution of glutamic acid at position 176 by glycine (e176g), were identified to result in the loss of n-p interaction at 37 de ... | 2002 | 11861854 |
| targeted therapy of respiratory syncytial virus in african green monkeys by intranasally administered 2-5a antisense. | respiratory syncytial virus (rsv) is a leading cause of respiratory disease in infants, young children, immunocompromised patients, and the institutionalized elderly. previous work had shown that rnase l, an antiviral enzyme of the interferon system, could be recruited to cleave rsv genomic rna by attaching tetrameric 2prime prime or minute-5prime prime or minute-linked oligoadenylates (2-5a) to an oligonucleotide complementary to repetitive gene-start sequences within the rsv genome (2-5a antis ... | 2002 | 11878909 |
| diagnosis of respiratory syncytial virus infection: comparison of reverse transcription-pcr to viral culture and serology in adults with respiratory illness. | diagnosis of respiratory syncytial virus (rsv) during acute infection in adults is difficult because of the poor sensitivity of viral culture and antigen detection. a recently developed single-tube nested reverse transcription-pcr (rt-pcr) was compared to viral culture and serology by enzyme immunoassay for the diagnosis of rsv in adults with respiratory illness. nasal swab samples were collected during respiratory illnesses from five groups of subjects: healthy young adults, healthy elderly adu ... | 2002 | 11880399 |
| the role of ifn in respiratory syncytial virus pathogenesis. | formalin-inactivated respiratory syncytial virus (rsv) vaccine preparations have been shown to cause enhanced disease in naive hosts following natural infection. in this study we demonstrate a similar pattern of enhanced disease severity following primary rsv infection of ifn-nonresponsive stat1(-/-) mice. stat1(-/-) mice showed markedly increased illness compared with wild-type balb/c animals following rsv inoculation despite similar lung virus titers and rates of virus clearance. histologicall ... | 2002 | 11884466 |
| nosocomial pneumonia in pediatric patients: practical problems and rational solutions. | nosocomial pneumonia is a common hospital-acquired infection in children, and is often fatal. risk factors for nosocomial pneumonia include admission to an intensive care unit, intubation, burns, surgery, and underlying chronic illness. viruses, predominantly respiratory syncytial virus (rsv), are the most common cause of pediatric nosocomial respiratory tract infections. gram-negative bacteria (escherichia coli, klebsiella pneumoniae, and pseudomonas aeruginosa) are the predominant bacterial pa ... | 2002 | 11888355 |
| respiratory syncytial virus infection induces matrix metalloproteinase-9 expression in epithelial cells. | increased gelatinolytic activity was observed in respiratory syncytial virus (rsv)-infected hep-2 cells by using zymography. the anti-matrix metalloproteinase-9 (mmp-9) antibody specifically reduced the gelatinolytic activity suggesting that the increased gelatinolytic activity was due to the mmp-9. it was also supported by the results from immunofluorescent staining, treatment of mmp inhibitors, and rsv infection of the cell clones that were transfected with plasmids to express more mmp-9 and t ... | 2002 | 11890521 |
| respiratory syncytial virus infections in children. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory disease in young children in both developing and developed countries. by age 2, nearly all children have been infected by rsv.the clinical manifestations range from mild upper respiratory symptoms to bronchiolitis and pneumonia. first infections are nearly always symptomatic and frequently cause lower respiratory tract disease, whereas subsequent infections are generally milder. although children with underlying conditio ... | 2002 | 11891515 |
| respiratory syncytial virus infection following hematopoietic stem cell transplantation. | respiratory syncytial virus, one of the most common causes of respiratory infections in immunocompetent individuals, is frequently spread to recipients of hsct by family members, other patients, and health care workers. in immunosuppressed individuals, progression from upper respiratory tract disease to pneumonia is common, and usually fatal if left untreated. we performed a retrospective analysis of rsv infections in recipients of autologous or allogeneic transplants. the incidence of rsv follo ... | 2002 | 11896429 |
| the failure of interleukin-10-deficient mice to develop airway hyperresponsiveness is overcome by respiratory syncytial virus infection in allergen-sensitized/challenged mice. | interleukin-10-deficient mice develop a robust pulmonary inflammatory response but no airway hyperresponsiveness (ahr) to inhaled methacholine (mch) following allergen sensitization and challenge. in the present study, we investigated the effect of respiratory syncytial virus (rsv) infection on ahr and pulmonary inflammation in allergic il-10-/- mice. unlike littermate control mice, rsv-infected or ovalbumin (ova)-sensitized/challenged il-10-/- mice failed to develop significant ahr. in contrast ... | 2002 | 11897651 |
| characteristics of a respiratory syncytial virus persistently infected macrophage-like culture. | a persistently infected culture obtained from immortalized murine macrophage-like cells, which survived respiratory syncytial virus (rsv) infection at multiplicity of one, was established and characterized. the presence of rsv through the passages was confirmed and monitored by (a) detection of infectious virus by tcid(50)/ml, (b) defective particles by viral infectivity interference and buoyant density determinations, (c) cell surface antigen by indirect immunofluorescence and facs, and (d) exp ... | 2002 | 11900838 |
| respiratory syncytial virus infection and the primary care physician. | respiratory syncytial virus (rsv) infection is a common viral illness affecting almost all children within their first few years of life. in most young children, rsv results in a mild respiratory infection. it is, however, the single most important cause of bronchiolitis and pneumonitis in infancy and contributes to significant morbidity and even mortality in a subset of high-risk children. there are new developments in the diagnosis, treatment, and prevention of rsv infection in infants and chi ... | 2002 | 11902705 |
| [humanized respiratory syncytial virus monoclonal antibody]. | respiratory syncytial virus remains a significant cause of severe lower respiratory tract disease in children. the risk of serious rsv illness is highest among children with prematurity, chronic lung disease and congenital heart disease. no effective vaccine and anti-viral agents have been obtained even now. therefore, conservative therapy including respiratory aid has been a principal therapy for serious rsv disease. recently, monthly intramuscular administration of humanized anti-rsv monoclona ... | 2002 | 11904963 |
| respiratory syncytial virus matrix protein associates with nucleocapsids in infected cells. | little is known about the functions of the matrix (m) protein of respiratory syncytial virus (rsv). by analogy with other negative-strand rna viruses, the m protein should inhibit the viral polymerase prior to packaging and facilitate virion assembly. in this study, localization of the rsv m protein in infected cells and its association with the rsv nucleocapsid complex was investigated. rsv-infected cells were shown to contain characteristic cytoplasmic inclusions. further analysis showed that ... | 2002 | 11907323 |
| genetic susceptibility to respiratory syncytial virus infection in inbred mice. | differences in the severity of respiratory syncytial virus (rsv)-induced lower respiratory disease in infants have been attributed to multiple environmental and genetic factors. to identify the genetic factor(s) influencing rsv susceptibility, we examined rsv infection in eight inbred mouse strains. lung rsv titers differed significantly between mouse strains: the rsv titers were 15-fold higher in akr/j (permissive) mice compared with c57bl/6j (resistant) mice at 4 days after inoculation. this s ... | 2002 | 11920822 |
| respiratory syncytial virus and tnf alpha induction of chemokine gene expression involves differential activation of rel a and nf-kappa b1. | respiratory syncytial virus (rsv) infection of airway epithelial cells stimulates the expression and secretion of a variety of cytokines including the chemotactic cytokines interleukin-8 (il-8), monocyte chemoattractant protein-1 (mcp-1), and rantes (regulated upon activation, normal t cell expressed and secreted). chemokines are important chemoattractants for the recruitment of distinct sets of leukocytes to airway sites of inflammation. | 2002 | 11922866 |
| [guidelines for respiratory syncytial virus prophylaxis. an update]. | epidemiological studies performed by the iris study group in the last two respiratory syncytial virus (rsv) seasons found that the hospitalization rates for rsv in premature infants born before or in week 32 of gestation were 13.4 % and 13.1 %, respectively. of these, 18 % and 25 % of the infants were admitted to the intensive care unit. currently available information demonstrates the efficacy of rsv monoclonal antibodies (palivizumab) and the absence of major adverse effects. to date, there ar ... | 2002 | 11927077 |
| [chemoprophylaxis with palivizumab in andalusia (spain). results of the 2000-2001 respiratory syncytial virus epidemic]. | to assess the hospitalization rate for bronchiolitis in newborns treated with palivizumab in andalusia. | 2002 | 11927095 |
| concurrent serious bacterial infections in 2396 infants and children hospitalized with respiratory syncytial virus lower respiratory tract infections. | at driscoll children's hospital (corpus christi, tex), we observed that most infants and children hospitalized for treatment of respiratory syncytial virus (rsv) bronchiolitis and/or pneumonia received broad-spectrum intravenous antibiotics despite having typical rsv signs and symptoms and positive rsv-rapid-antigen tests on admission. physicians were concerned about the possibility of concurrent serious bacterial infections, especially in infants younger than 3 months and in those with infiltra ... | 2002 | 11929363 |
| illness severity, viral shedding, and antibody responses in infants hospitalized with bronchiolitis caused by respiratory syncytial virus. | the relationships between host factors, viral shedding, illness severity, and antibody response in respiratory syncytial virus (rsv)-induced bronchiolitis are poorly defined. these relationships were prospectively evaluated in 77 infants hospitalized with rsv bronchiolitis in multicenter, double-blind, placebo-controlled trials of rsv immunoglobulin therapy. severity of illness was influenced by age and host risk factors but was not influenced by rsv neutralizing antibody titer or by the amount ... | 2002 | 11930309 |
| respiratory syncytial virus (rsv) nonstructural (ns) proteins as host range determinants: a chimeric bovine rsv with ns genes from human rsv is attenuated in interferon-competent bovine cells. | bovine respiratory syncytial virus (brsv) escapes from cellular responses to alpha/beta interferon (ifn-alpha/beta) by a concerted action of the two viral nonstructural proteins, ns1 and ns2. here we show that the ns proteins of human rsv (hrsv) are also able to counteract ifn responses and that they have the capacity to protect replication of an unrelated rhabdovirus. even combinations of brsv and hrsv ns proteins showed a protective activity, suggesting common mechanisms and cellular targets o ... | 2002 | 11932394 |
| nk t cells contribute to expansion of cd8(+) t cells and amplification of antiviral immune responses to respiratory syncytial virus. | cd1d-deficient mice have normal numbers of t lymphocytes and natural killer cells but lack valpha14(+) natural killer t cells. respiratory syncytial virus (rsv) immunopathogenesis was evaluated in 129xc57bl/6, c57bl/6, and balb/c cd1d(-/-) mice. cd8(+) t lymphocytes were reduced in cd1d(-/-) mice of all strains, as shown by cell surface staining and major histocompatibility complex class i tetramer analysis, and resulted in strain-specific alterations in illness, viral clearance, and gamma inter ... | 2002 | 11932395 |
| infections and asthma. | infections have a variety of influences in the asthmatic patient. respiratory syncytial virus (rsv) is the most common cause of wheezing illnesses in children under the age of 2 years and may be a risk factor for the inception of asthma in the first decade of life. once asthma has become recognized clinically, rhinovirus is the leading cause of acute asthma exacerbations in adults and older children. certain respiratory infections may induce chronic lower airway inflammation that may contribute ... | 2002 | 16088627 |
| respiratory syncytial virus and chlamydia are not detectable by pcr in ongoing vernal keratoconjunctivitis. | respiratory syncytial virus (rsv) and chlamydial infection may be pathogenetic factors in allergic diseases, perhaps also in ocular allergy. we analyzed the presence of rsv and chlamydial nucleic acids using reverse transcription (rt)-pcr and pcr, respectively, in conjunctival biopsies from patients with vernal keratoconjunctivitis (vkc) in order to determine whether these agents play a role in the maintenance of the disease. all biopsy samples were negative for both rsv (n = 15 for vkc and n = ... | 2001 | 11935435 |
| vaccine prevention of acute otitis media. | the incidence of acute otitis media (aom) in infants and young children has increased dramatically in recent years in the united states. aom often follows upper respiratory tract infections due to pathogens such as respiratory syncytial virus (rsv), influenza virus, and parainfluenza virus (piv). these viruses cause eustachian tube dysfunction that is critical to the pathogenesis of aom. vaccines against these viruses would likely reduce the incidence of aom. in three previous studies, influenza ... | 2001 | 11892059 |
| the organization of mature rous sarcoma virus as studied by cryoelectron microscopy. | we have studied the organization of mature infectious rous sarcoma virus (rsv), suspended in vitreous ice, using transmission electron microscopy. the enveloped virions are spherical in shape, have a mean diameter of 127 nm, and vary significantly in size. image processing reveals the presence of the viral matrix protein underlying the lipid bilayer and the viral envelope proteins external to the lipid bilayer. in the interior of the virus, the characteristic mature retroviral core is clearly im ... | 2001 | 11858708 |
| [bronchiolitis caused by influenza type a virus. an emerging infectious disease]. | to prospectively study the emergence and diagnostic problems of bronchiolitis caused by influenza a virus among children durign the 1999-2000 influenza epidemic. | 2001 | 11844450 |
| the development of a mimotope-based synthetic peptide vaccine against respiratory syncytial virus. | respiratory syncytial virus (rsv) is the most important cause of bronchiolitis and pneumonia in infants and young children worldwide and the development of a synthetic peptide epitope-based vaccine to induce virus-neutralising antibodies against rsv would seem to be a valid approach to the production of an effective vaccine against infection.a combinatorial solid-phase peptide library has been screened with a virus-neutralising, protective monoclonal antibody (mab19) directed towards a conserved ... | 2001 | 11851318 |
| paramyxovirus accessory proteins as interferon antagonists. | a new role of the paramyxovirus accessory proteins has been uncovered. the p gene of the subfamily paramyxovirinae encodes accessory proteins including the v and/or c protein by means of pseudotemplated nucleotide addition (rna editing) or by overlapping open reading frame. the respirovirus (sendai virus and human parainfluenza virus (hpiv)3) and rubulavirus (simian virus (sv)5, sv41, mumps virus and hpiv2) circumvent the interferon (ifn) response by inhibiting ifn signaling. the responsible gen ... | 2001 | 11838896 |
| [viral respiratory infections as cause of fever in hospitalized aged patients during a winter season]. | in the geriatric units of the university hospital of saint-etienne, 129 cases of fever (38 degrees c or more) were recorded prospectively during the 1995-1996 winter period in a population of 503 hospitalised patients (25.6%), and were investigated for the detection of a viral aetiology. | 2001 | 11794888 |
| relationship between respiratory syncytial virus bronchiolitis and future obstructive airway diseases. | evidence from a large number of prospective case-control studies shows that respiratory syncytial virus (rsv) bronchiolitis in infancy is often associated with recurrent wheezing and asthma during subsequent years. however, wheezing tends to diminish and most studies show no significant increase in wheezing compared to controls by school age or adolescence. an unresolved question is whether severe rsv infection during infancy causes the respiratory sequelae or inherent abnormalities predispose a ... | 2001 | 11829086 |
| ras/map kinase pathway is associated with the control of myotube formation but not myofibril assembly in quail myoblasts transformed with rous sarcoma virus. | tyrosine kinase activity of v-src from rous sarcoma virus (rsv) inhibits the differentiation of quail myoblasts. to clarify the inhibitory mechanism, we focused on the signaling pathways from v-src. when the activation of the ras/map (mitogen-activated protein) kinase pathway was inhibited by a dominant-negative mutant of ras or pd98059, a specific inhibitor of p42 map kinase kinase, differentiation was restored; muscle specific proteins were expressed and myotubes formed even under active condi ... | 2001 | 11831357 |
| epithelial cells infected with respiratory syncytial virus are resistant to the anti-inflammatory effects of hydrocortisone. | in this work we continue our study of the biochemical responses of respiratory epithelial cells to infection with human paramyxovirus pathogens. in our earlier studies, we detected elevated concentrations of the proinflammatory chemokines mip-1alpha and il-8 in upper and lower respiratory tract secretions from patients infected with respiratory syncytial virus (rsv). here we demonstrate the same trend for individuals infected with parainfluenza virus (piv), with elevated concentrations of mip-1a ... | 2001 | 11831875 |
| clinical characteristics of respiratory syncytial virus infection in hospitalized healthy infants and young children in qatar. | to evaluate seasonal trends, clinical profile, and outcome of disease in previously healthy infants and young children hospitalized for respiratory syncytial virus (rsv) infection at hamad medical corporation in the state of qatar, we reviewed the records of 257 children admitted between 1 january 1996 and 31 december 1998. rsv epidemics occurred yearly during the winter months with peak hospitalizations occuring between november and february. of the 257 admissions, 160 (62.3 per cent) were male ... | 2001 | 11827306 |
| community-acquired respiratory syncytial virus and parainfluenza virus infections after hematopoietic stem cell transplantation: the fred hutchinson cancer research center experience. | community respiratory viruses (crvs) are an important cause of morbidity and mortality among recipients of hematopoietic stem cell transplants (hsct). at the fred hutchinson cancer research center, respiratory syncytial virus (rsv) and parainfluenza virus (piv) infections in hsct recipients have been studied intensively for more than a decade. over time, mortality from these infections has declined as the approach to diagnosis has become more aggressive and more stringent preventive measures hav ... | 2001 | 11777098 |
| preemptive treatment of pediatric bone marrow transplant patients with asymptomatic respiratory syncytial virus infection with aerosolized ribavirin. | respiratory syncytial virus (rsv) is a cause of serious respiratory infections in pediatric patients. rsv infection may be especially devastating in pediatric bone marrow transplant (bmt) recipients. because of the high mortality attributed to rsv lower respiratory tract infection, a pilot study of preemptive treatment of asymptomatic rsv shedding in pediatric bmt recipients was conducted. nasopharyngeal wash specimens from 25 pediatric bmt recipients were screened for rsv infection prior to pat ... | 2001 | 11777099 |
| guidelines for preventing opportunistic infections among hematopoietic stem cell transplant recipients: focus on community respiratory virus infections. | guidelines for preventing opportunistic infections among hematopoietic stem cell transplant (hsct) recipients, cosponsored by the centers for disease control and prevention, the infectious diseases society of america, and the american society for blood and marrow transplantation, were issued in october 2000. the guidelines recommend that to minimize transmission of community respiratory virus (crv) infection, health care workers and visitors with symptoms of upper respiratory tract infection be ... | 2001 | 11777100 |
| respiratory virus infections in stem cell transplant patients: the european experience. | the frequency of and survival from community-acquired respiratory virus (crv) infections among patients undergoing allogeneic or autologous stem cell transplantation (sct) were evaluated in a prospective study conducted at 37 medical centers affiliated with the european group for blood and marrow transplantation. of the 40 crv infections diagnosed in 1863 patients (739, allogeneic sct; 1124, autologous sct), 20 were attributed to respiratory syncytial virus (rsv), 4 to parainfluenza viruses, and ... | 2001 | 11777102 |
| influenza diagnosis and treatment: a view from clinical practice. | influenza is a descriptive term for respiratory epidemic disease presenting with cough and fever. influenza viruses are probably the most important of the pathogens that cause this condition. clinical influenza occurs almost every winter in england and wales and the outbreaks last 8-10 weeks. in recent years, influenza b virus outbreaks have occurred in january and february, whereas influenza h3n2 virus outbreaks have generally started long before christmas. influenza h3n2 virus outbreaks pressu ... | 2001 | 11779394 |
| molecular epidemiology of respiratory syncytial virus. | to determine the epidemiologic pattern of subgroups a and b and genotypes of respiratory syncytial virus (rsv) during two noncontinuous epidemics during 1990-1991 and 1997-1998 in beijing. | 2001 | 11780455 |
| pre-emptive oral ribavirin therapy of paramyxovirus infections after haematopoietic stem cell transplantation: a pilot study. | infections with the paramyxoviruses, respiratory syncytial virus (rsv) and parainfluenza virus (piv) can result in serious morbidity and mortality after haemopoietic stem cell transplant (hsct). once pneumonia develops, the outcome of these infections is often poor despite anti-viral therapy. aerosolised ribavirin has been evaluated as pre-emptive therapy for post-transplant rsv infections with some success. due to the financial and logistic burden involved with the use of aerosolised ribavirin, ... | 2001 | 11781627 |
| human respiratory syncytial virus surface glycoproteins f, g and sh form an oligomeric complex. | in order to study structural associations, rsv surface glycoproteins were evaluated using heparin agarose affinity chromatography (haac). when rsv-infected cell lysate was analyzed by haac, all three surface glycoproteins, (f, g and sh), were eluted. similarly, when separate lysates from vero cells infected with vaccinia recombinants expressing f (vvf), g (vvg) and sh (vvsh) proteins were subjected to haac, only vvf and vvg expressed proteins bound to heparin, whereas vvsh expressed protein did ... | 2001 | 11811686 |
| update on the development and use of viral and bacterial vaccines for the prevention of acute otitis media. | acute otitis media (aom) is the most frequent diagnosis in physician offices among children 1-4 years of age. viruses that cause upper respiratory tract infections (i.e., respiratory syncytial virus [rsv], influenza virus, parainfluenza virus [piv], and others) play an important role in the development of aom. prevention of infections with these viral pathogens likely would reduce the incidence of aom. in three previous studies, influenza virus vaccines showed 30-36% efficacy against the develop ... | 2001 | 11775392 |
| preventive and therapeutic strategies for respiratory syncytial virus infection. | respiratory syncytial virus (rsv) remains an important cause of pneumonitis in infants and in the elderly and immunosuppressed. passive immunoprophylaxis of high-risk infants with the humanised monoclonal antibody palivizumab reduces rsv-related hospitalisation and admission to intensive care units by about 50%, although optimal and cost-effective use of this agent remains undefined. the development of an rsv vaccine is the focus of much research and recent advances with live attenuated vaccines ... | 2001 | 11764776 |
| bronchiolitis in inuit children from a canadian central arctic community, 1995-1996. | during the winter and summer of 1996 two outbreaks of bronchiolitis occurred among inuit children in the canadian arctic community of arviat. the epidemiology and clinical features of these epidemics were studied through a chart review of bronchiolitis cases occurring from september 1, 1995 to august 31, 1996. the attack rate over the year was 57% and the incidence was 1.25 cases per child-year at risk. the median age of cases was 9 months. children developing bronchiolitis were more likely to b ... | 2001 | 11768447 |
| community well being and infectious diseases among alaska native communities in the chugach region. | this study sought to examine how native people of the chugach region of alaska perceive their own communities' health and well being, particularly in regard to infectious diseases. | 2001 | 11768448 |
| association of tobacco smoke exposure and respiratory syncitial virus infection with airways reactivity in early childhood. | exposure to infectious agents and environmental tobacco smoke are thought to induce bronchial hyperresponsiveness (bhr). this study was undertaken to determine the effects of passive exposure to tobacco smoke and respiratory syncitial virus (rsv) lower respiratory infection (lri) during infancy on the occurrence of bhr in the first 2 years of life. eighty-six cases of documented rsv (mean age, 188 days) and 78 controls (mean age, 162 days) were enrolled from the clinic and in-patient service of ... | 2001 | 11747244 |
| in situ prostate cancer gene therapy using a novel adenoviral vector regulated by the caveolin-1 promoter. | caveolin-1, a structural component of caveolae, is overexpressed in metastatic and androgen-resistant prostate cancer and highly expressed in tumor-associated endothelial cells. the mouse cav-1 promoter was cloned and placed upstream of the hsv-tk gene in an adenoviral vector (adcav-1tk) and compared with a cytomegalovirus (cmv) or rous sarcoma virus (rsv) promoter-driven hsv-tk, adcmvtk and adrsvtk vectors, respectively. mouse and human prostate cancer cells and mouse endothelial cells were inf ... | 2001 | 11751529 |
| mucosal delivery of a respiratory syncytial virus ctl peptide with enterotoxin-based adjuvants elicits protective, immunopathogenic, and immunoregulatory antiviral cd8+ t cell responses. | in an effort to develop a safe and effective vaccine against respiratory syncytial virus (rsv), we used escherichia coli heat-labile toxin (lt), and ltk63 (an lt mutant devoid of adp-ribosyltransferase activity) to elicit murine cd8(+) ctl responses to an intranasally codelivered ctl peptide from the second matrix protein (m2) of rsv. m2(82-90)-specific cd8(+) t cells were detected by ifn-gamma enzyme-linked immunospot and (51)cr release assay in local and systemic lymph nodes, and their inducti ... | 2001 | 11145691 |
| macrophage inflammatory protein-1alpha (not t helper type 2 cytokines) is associated with severe forms of respiratory syncytial virus bronchiolitis. | it has been suggested that the pathogenesis of respiratory syncytial virus (rsv) infection is related to the development of t helper (th) type 2 cytokine responses. the presence of th1 and th2 cytokines and the chemokines macrophage inflammatory protein (mip)-1alpha and monocyte chemotactic protein (mcp)-1 were assessed by elisa in nasopharyngeal secretions of infants with rsv infection. infants with mild bronchiolitis had increased th1 cytokines and reduced th2 cytokines, compared with infants ... | 2001 | 11471095 |
| [viral infection of the lower respiratory tract in hospitalized infants: etiology, clinical features and risk factors]. | the aim of this study was to assess the clinical and virological characteristics of lower respiratory tract (lrt) infection in hospitalized infants in spain and to identify clinical, radiological or laboratory parameters that could, on admission, be associated with a more severe clinical course. | 2001 | 11472660 |
| il-13-induced airway hyperreactivity during respiratory syncytial virus infection is stat6 dependent. | airway damage and hyperreactivity induced during respiratory syncytial virus (rsv) infection can have a prolonged effect in infants and young children. these infections can alter the long-term function of the lung and may lead to severe asthma-like responses. in these studies, the role of il-13 in inducing and maintaining a prolonged airway hyperreactivity response was examined using a mouse model of primary rsv infection. using this model, there was evidence of significant airway epithelial cel ... | 2001 | 11207314 |
| cpg island protects rous sarcoma virus-derived vectors integrated into nonpermissive cells from dna methylation and transcriptional suppression. | cpg islands are important in the protection of adjacent housekeeping genes from de novo dna methylation and for keeping them in a transcriptionally active state. however, little is known about their capacity to protect heterologous genes and assure position-independent transcription of adjacent transgenes or retroviral vectors. to tackle this question, we have used the mouse aprt cpg island to flank a rous sarcoma virus (rsv)-derived reporter vector and followed the transcriptional activity of i ... | 2001 | 11209056 |
| antiviral amentoflavone from selaginella sinensis. | amentoflavone and three other flavonoids were isolated from the ethanol extract of selaginella sinensis. amentoflavone showed potent antiviral activity against respiratory syncytial virus (rsv), with an ic50 of 5.5 microg/ml. the contents of amentoflavone in nine species of selaginella were determined by reversed-phase hplc. s. sinensis showed a higher content of 1.13%. | 2001 | 11256492 |
| antiviral activity of lovastatin against respiratory syncytial virus in vivo and in vitro. | respiratory syncytial virus (rsv) is an important human pathogen that can cause severe and life-threatening respiratory infections in infants and immunocompromised adults. we have recently shown that the rsv f glycoprotein, which mediates viral fusion, binds to rhoa. one of the steps in rhoa activation involves isoprenylation at the carboxy terminus of the protein by geranylgeranyltransferase. this modification allows rhoa to be attached to phosphatidyl serine on the inner leaflet of the plasma ... | 2001 | 11257039 |
| identification and characterisation of multiple linear b cell protectopes in the respiratory syncytial virus g protein. | respiratory syncytial virus (rsv) is an important respiratory pathogen in man, against which no vaccine is available. however, recent evidence suggests that antibodies to the rsv f and g proteins may play an important role in disease prevention. we previously demonstrated that bbg2na, a subunit vaccine candidate including residues 130-230 of the long strain g protein, protects rodents against rsv challenge. using a panel of monoclonal antibodies (mab) and synthetic peptides, five linear b cell e ... | 2001 | 11257359 |
| oxidant tone regulates rantes gene expression in airway epithelial cells infected with respiratory syncytial virus. role in viral-induced interferon regulatory factor activation. | respiratory syncytial virus (rsv) produces intense pulmonary inflammation, in part, through its ability to induce chemokine synthesis in infected airway epithelial cells. rantes (regulated upon activation, normal t-cells expressed and secreted) is a cc chemokine which recruits and activates monocytes, lymphocytes, and eosinophils, all cell types present in the lung inflammatory infiltrate induced by rsv infection. in this study we investigated the role of reactive oxygen species in the induction ... | 2001 | 11259439 |
| 2001 aspet otto krayer award lecture. molecular targets for antiviral agents. | there are a number of virus-specific processes within the virus replicative cycle or virus-infected cell that have proven to be attractive targets for chemotherapeutic intervention, i.e., virus adsorption and entry into the cells, reverse (rna --> dna) transcription, viral dna polymerization, and cellular enzymatic reactions that are associated with viral dna and rna synthesis and viral mrna maturation (i.e., methylation). a variety of chemotherapeutic agents, both nucleoside (and nucleotide) an ... | 2001 | 11259521 |
| molecular epidemiology of respiratory syncytial virus. | human respiratory syncytial virus (rsv) is the major cause of lower respiratory tract disease in infants. it is unusual in that it causes repeated infections throughout life. despite considerable efforts there is as yet no satisfactory vaccine available. this paper reviews the molecular epidemiology of the rsv and describes the complex genotypic structure of rsv epidemics. the evolution of the virus is discussed, with particular reference to the antigenic and genetic variability of the attachmen ... | 2001 | 11262529 |
| characterization of monoclonal antibodies raised against recombinant respiratory syncytial virus nucleocapsid (n) protein: identification of a region in the carboxy terminus of n involved in the interaction with p protein. | to investigate structure and biological properties of the nucleocapsid (n) protein of respiratory syncytial virus (rsv), we have generated a panel of 16 monoclonal antibodies, raised against recombinant n protein, and epitope mapped seven of these to three antigenic sites (site i aa 16-30; site ii aa 341-350; site iii aa 351-365). characterization by immunofluorescence and by immunoprecipitation assay demonstrated that a monoclonal antibody to antigenic site i can detect n protein complexed with ... | 2001 | 11689048 |
| contribution of the respiratory syncytial virus g glycoprotein and its secreted and membrane-bound forms to virus replication in vitro and in vivo. | the surface glycoproteins of viruses can play important roles in viral attachment, entry, and morphogenesis. here, we investigated the role of the attachment g glycoprotein of human respiratory syncytial virus (rsv) in viral infection. rsv g is produced both as a complete, transmembrane form and as an n-terminally truncated form that is secreted. using reverse genetics, we created mutant recombinant rsvs (rrsv) that do not express g (deltag) or express either the secreted or the membrane-bound f ... | 2001 | 11689051 |
| requirement of cysteines and length of the human respiratory syncytial virus m2-1 protein for protein function and virus viability. | the m2-1 protein of human respiratory syncytial virus (hrsv) promotes processive rna synthesis and readthrough at rsv gene junctions. it contains four highly conserved cysteines, three of which are located in the cys(3)-his(1) motif at the n terminus of m2-1. each of the four cysteines, at positions 7, 15, 21, and 96, in the m2-1 protein of hrsv a2 strain was individually replaced by glycines. when tested in an rsv minigenome replicon system using beta-galactosidase as a reporter gene, c7g, c15g ... | 2001 | 11689613 |
| palmitoylation of the rous sarcoma virus transmembrane glycoprotein is required for protein stability and virus infectivity. | the rous sarcoma virus (rsv) transmembrane (tm) glycoprotein is modified by the addition of palmitic acid. to identify whether conserved cysteines within the hydrophobic anchor region are the site(s) of palmitoylation, and to determine the role of acylation in glycoprotein function, cysteines at residues 164 and 167 of the tm protein were mutated to glycine (c164g, c167g, and c164g/c167g). in cv-1 cells, palmitate was added to env gene products containing single mutations but was absent in the d ... | 2001 | 11689636 |
| immunostimulatory dna sequences inhibit respiratory syncytial viral load, airway inflammation, and mucus secretion. | immunostimulatory dna sequences (iss) activate the innate immune system to generate antiviral cytokines, such as ifn-gamma. | 2001 | 11692091 |
| eosinophils, eosinophil ribonucleases, and their role in host defense against respiratory virus pathogens. | eosinophils remain among the most enigmatic of cells, as our appreciation of their detrimental activities--e.g., asthma and allergic disease--far outweighs our understanding of their beneficial effects. among the major secretory effector proteins of eosinophils are the ribonucleases eosinophil-derived neurotoxin (edn) and eosinophil cationic protein (ecp) in primates and their orthologs, the eosinophil-associated ribonucleases (ears) in rodents. the rapid diversification observed among these rib ... | 2001 | 11698487 |
| contribution of influenza and respiratory syncytial virus to community cases of influenza-like illness: an observational study. | respiratory syncytial virus (rsv) is an important cause of lower-respiratory-tract infection in children and elderly people, but its effect in other age-groups is uncertain. we did a community-based observational study of rsv infection in community-dwelling individuals of all ages who presented to general practices in the uk with influenza-like illnesses during three successive winters (1995-96, 1996-97, and 1997-98). | 2001 | 11705487 |
| immune interaction between respiratory syncytial virus infection and allergen sensitization critically depends on timing of challenges. | severe respiratory syncytial virus (rsv) infection has been hypothesized to be a risk factor for the development of allergy and asthma, but epidemiologic studies in humans have been inconclusive. by use of a well-characterized murine model of rsv infection and allergic sensitization with ovalbumin, the effect of a preceding severe rsv infection on the development of the pulmonary allergic inflammatory response and airway hyperresponsiveness (ahr) was tested. the impact of prior allergic sensitiz ... | 2001 | 11709778 |
| safety and immunogenicity of a novel recombinant subunit respiratory syncytial virus vaccine (bbg2na) in healthy young adults. | a novel recombinant respiratory syncytial virus (rsv) subunit vaccine, designated bbg2na, was administered to 108 healthy adults randomly assigned to receive 10, 100, or 300 microg of bbg2na in aluminum phosphate or saline placebo. each subject received 1, 2, or 3 intramuscular injections of the assigned dose at monthly intervals. local and systemic reactions were mild, and no evidence of harmful properties of bbg2na was reported. the highest elisa and virus-neutralizing (vn) antibody responses ... | 2001 | 11709789 |
| granulocyte-macrophage colony-stimulating factor expressed by recombinant respiratory syncytial virus attenuates viral replication and increases the level of pulmonary antigen-presenting cells. | an obstacle to developing a vaccine against human respiratory syncytial virus (rsv) is that natural infection typically does not confer solid immunity to reinfection. to investigate methods to augment the immune response, recombinant rsv (rrsv) was constructed that expresses murine granulocyte-macrophage colony-stimulating factor (mgm-csf) from a transcription cassette inserted into the g-f intergenic region. replication of rrsv/mgm-csf in the upper and lower respiratory tracts of balb/c mice wa ... | 2001 | 11711604 |
| differential histopathology and chemokine gene expression in lung tissues following respiratory syncytial virus (rsv) challenge of formalin-inactivated rsv- or bbg2na-immunized mice. | a balb/c mouse model of enhanced pulmonary pathology following vaccination with formalin-inactivated alum-adsorbed respiratory syncytial virus (fi-rsv) and live rsv challenge was used to determine the type and kinetics of histopathologic lesions induced and chemokine gene expression profiles in lung tissues. these data were compared and contrasted with data generated following primary and/or secondary rsv infection or rsv challenge following vaccination with a promising subunit vaccine, bbg2na. ... | 2001 | 11711632 |
| dnase treatment for atelectasis in infants with severe respiratory syncytial virus bronchiolitis. | respiratory insufficiency due to respiratory syncytial virus (rsv) bronchiolitis is partly due to the abundance of thickened mucus and the inability to clear it from the airways. mucus in rsv bronchiolitis contains necrotic inflammatory and epithelial cells. the viscoelastic properties of purulent airway secretions are largely due to the presence of highly polymerized deoxyribonucleic acid (dna). recombinant human deoxyribonuclease (rhdnase) is known to liquefy such mucus in patients with cystic ... | 2001 | 11716180 |
| [primary respiratory syncytial virus infection in mice]. | to establish a mice-model of primary respiratory syncytial virus infection. | 2001 | 11718040 |
| respiratory viruses, symptoms, and inflammatory markers in acute exacerbations and stable chronic obstructive pulmonary disease. | the effects of respiratory viral infection on the time course of chronic obstructive pulmonary disease (copd) exacerbation were examined by monitoring changes in systemic inflammatory markers in stable copd and at exacerbation. eighty-three patients with copd (mean [sd] age, 66.6 [7.1] yr, fev(1), 1.06 [0.61] l) recorded daily peak expiratory flow rate and any increases in respiratory symptoms. nasal samples and blood were taken for respiratory virus detection by culture, polymerase chain reacti ... | 2001 | 11719299 |
| health care utilisation of infants with chronic lung disease, related to hospitalisation for rsv infection. | to compare the use of health care resources and associated costs between infants with chronic lung disease (cld) who had or had not an admission with a proven respiratory syncytial virus (rsv) infection. | 2001 | 11719328 |
| suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection. | interleukin (il)-10 is known to be a multifunctional cytokine. this study was designed to evaluate the role of il-10 during respiratory syncytial virus (rsv) infection using a c57bl/6 transgenic (tg) mouse model in which the expression of murine il-10 cdna was regulated by a human salivary amylase promoter (il-10 tg mice). these mice expressed a large amount of il-10 in the nasal mucosa and in salivary glands. viral replication in the respiratory tract after intranasal infection with rsv was sup ... | 2001 | 11722651 |
| the ovine respiratory syncytial virus f gene sequence and its diagnostic application. | ruminant respiratory syncytial viruses (rsvs) are classified into 2 subgroups, ovine rsv and bovine rsv. although ovine rsv infects cattle, its contribution to bovine respiratory tract disease has not been established, which is an important issue for vaccine development in cattle. diagnosis by virus isolation or serology has low or variable sensitivity and/or specificity and polymerase chain reaction (pcr) has been recommended as a rapid and sensitive technique for rsv detection. a simple proced ... | 2001 | 11724134 |
| in vitro evaluation of secoiridoid glucosides from the fruits of ligustrum lucidum as antiviral agents. | six secoiridoid glucosides, lucidumoside c (1), oleoside dimethylester (2), neonuezhenide (3), oleuropein (4), ligustroside (5) and lucidumoside a (6), isolated from the fruits of ligustrum lucidum (oleaceae), were examined in vitro for their activities against four strains of pathogenic viruses, namely herpes simplex type i virus (hsv-1), influenza type a virus (flu a), respiratory syncytial virus (rsv) and parainfluenza type 3 virus (para 3). antiviral activities were evaluated by the cytopath ... | 2001 | 11724241 |
| clinical protocol. purging of autologous stem cell sources with bcl-x(s) adenovirus for women undergoing high-dose chemotherapy for stage iv breast carcinoma. | high-dose chemotherapy (hdct) and autologous bone marrow transplantation (bmt) is frequently used to treat patients with metastatic cancer including breast cancer and neuroblastoma. however, the bone marrow of such patients is often contaminated with tumor cells. recently, we have found that a recombinant adenovirus vector that contains a bcl-x, minigene (a dominant negative inhibitor of the bcl-2 family), called the bcl-x(s) adenovirus, is lethal to cancer cells derived from epithelial tissues, ... | 2001 | 11727734 |
| clinical profile of serologically diagnosed pneumococcal pneumonia. | to describe the characteristics of serologically diagnosed pneumococcal pneumonia and compare them with those of respiratory syncytial virus (rsv) pneumonia and bacteremic pneumococcal pneumonia. | 2001 | 11734706 |
| hospitalization rates for respiratory syncytial virus infection in premature infants born during two consecutive seasons. | to collect data on hospitalization rates for respiratory syncytial virus (rsv) illness during the season of 1999 to 2000 in nonprophylaxed premature infants < or = 32 weeks gestational age (ga) in spain and compare this with previously published data collected in the season of 1998 to 1999. | 2001 | 11734767 |
| respiratory syncytial virus infection of the lower respiratory tract: radiological findings in 108 children. | for years the typical appearance of respiratory syncytial virus (rsv)-induced infection of the lower respiratory tract has been discussed. all available studies have led to different results. the aim of this study was to control these results, with 108 children. the age range was 1 day to 10 years (median 7 months). within 72 h of admission, all children developed an rsv infection of the lower respiratory tract. chest x-rays (pa-view) of 55 children under, and 53 children over, the age of 6 mont ... | 2001 | 11734962 |
| risk factors for severe respiratory syncytial virus-associated lower respiratory tract infection in children. | respiratory syncytial virus (rsv) is the most important cause of viral lower respiratory tract infection that can be a life-threatening disease in infants and children. this study was conducted to look for independent risk factors for severe respiratory syncytial virus-associated lower respiratory tract infection (rsv-lri) that required oxygen supplementation or mechanical ventilation. | 2001 | 11737710 |
| respiratory syncytial virus vaccine: a systematic overview with emphasis on respiratory syncytial virus subunit vaccines. | to explore whether rsv vaccines are efficacious in preventing respiratory syncytial virus (rsv) lower respiratory infection (lri). | 2001 | 11738763 |
| glucocorticoid administration accelerates mortality of pneumovirus-infected mice. | the use of glucocorticoids for the treatment of symptoms associated with respiratory syncytial virus (rsv) infection has been questioned. to evaluate the sequelae of glucocorticoid administration in the setting of pneumovirus infection in vivo, hydrocortisone was administered to mice infected with pneumonia virus of mice (pvm), a pneumovirus and natural rodent pathogen that is closely related to rsv and replicates the signs and symptoms of severe human rsv infection. results showed that hydrocor ... | 2001 | 11740726 |
| cx3c chemokine mimicry by respiratory syncytial virus g glycoprotein. | chemokines are chemoattractant proteins that are divided into subfamilies based upon cysteine signature motifs termed c, cc, cxc and cx3c. chemokines have roles in immunity and inflammation that affect cell trafficking and activation of t cells as well as cells of the innate immune system. we report here cx3c chemokine mimicry for the g glycoprotein of respiratory syncytial virus (rsv) and show binding to cx3cr1--the specific receptor for the cx3c chemokine fractalkine--and induction of leukocyt ... | 2001 | 11477410 |