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effect of the prostaglandin analogue misoprostol on resistance to entamoeba histolytica infection in balb/c mice. 200011070249
entamoeba histolytica induces cyclooxygenase-2 expression in macrophages during amebic liver abscess formation. 200011070250
do entamoeba histolytica trophozoites signal via enteric microvilli? 200011070251
signal transduction pathways in entamoeba histolytica: pka activity and translocation during the interaction of trophozoites with fn. 200011070252
distribution of a potential p21-activated serine/threonine kinase (pak) in entamoeba histolytica. 200011070253
actin stress fibers in entamoeba histolytica induced by fibronectin. 200011070254
cytoskeleton activities during the interaction of entamoeba histolytica with epithelial cells. 200011070255
the diaphanous protein from entamoeba histolytica controls cell motility and cytokinesis. 200011070256
the interplay between receptor capping and cytoskeleton remodeling in entamoeba histolytica. 200011070257
immunolocalization of clathrin during electron-dense granule secretion in entamoeba histolytica. 200011070258
growth inhibition and actin aggregate formation of entamoeba histolytica by jasplakinolide. 200011070259
monoclonal antibody specific to the beta1 integrin-like molecule (140 kda) immunoprecipitates a protein complex of entamoeba histolytica. 200011070260
identification of collagen-binding proteins from the cytoplasmic membrane of entamoeba histolytica. 200011070261
molecular genetics of the secretory pathway in entamoeba histolytica: an overview. 200011070262
secretory pathway activation by interaction of entamoeba histolytica trophozoites with fibronectin. 200011070263
identification and characterizaton of a rab5 homologue in entamoeba histolytica. 200011070264
rab8 as a molecular model of vesicular trafficking to investigate the latter steps of the secretory pathway in entamoeba histolytica. 200011070265
cloning and functional expression of rpn1, a regulatory-particle non-atpase subunit 1, of proteasome from trypanosoma cruzi.non-lysosomal protein degradation in eukaryotic cells involves a proteolytic complex referred to as 26s proteasome that consists of a 20s core particle and one or two 19s regulatory particles. we have cloned the gene rpn1 encoding rpnl (regulatory-particle non-atpase subunit 1), one of the largest subunits of proteasome, from trypanosoma cruzi. it contains 2712 bp and encodes 904 amino acid residues with a calculated molecular mass of 98.2 kda and an isoelectric point of 5.2. the predicted amino ...200011071286
a simple and reliable 5'-race approach.a novel approach for the amplification of cdna ends is described. it requires only minimal amounts of material, a simple cdna synthesis reaction and a single pcr reaction to amplify the desired 5'- or 3'-ends of a certain cdna of interest. it combines the so called capfinder approach with solid phase cdna synthesis, thus almost eliminating background problems usually associated with 5'-race protocols. this approach could be used to generate complete 5'-ends of numerous cdnas using only one cdna ...200011071950
[diagnostic image (8). amebic colitis].a 34-year-old woman suffered from progressive abdominal pain and diarrhoea. laparotomy revealed colonic perforation due to amebic colitis.200011072508
entamoeba histolytica contains a ter-atpase. 200011070266
cloning of the entamoeba histolytica stt3 gene, a subunit of the oligosaccharyltransferase complex. 200011070267
ultrastructural evidence of smooth endoplasmic reticulum and golgi-like elements in entamoeba histolytica and entamoeba dispar. 200011070268
the endocytic pathway in entamoeba histolytica.we studied the endocytic pathway of entamoeba histolytica using (a) confocal laser scanning microscopy to observe living cells labeled with fluorescent probes and (b) transmission electron microscopy of cells incubated in the presence of gold-labeled proteins or in a cytochemical medium designed for the localization of acid phosphatase activity. images of acridine-orange-labeled cells showed that most of the intracellular vacuoles were acidic and were also labeled with lucifer yellow, a fluoresc ...200011097295
genomic organization of a 7 kb gene cluster from entamoeba histolytica. 200011070309
the application of the atomic force microscope to studies of medically important protozoan parasites.both living and fixed specimens of the medically-important parasitic protozoa, trypanosoma cruzi, toxoplasma gondii, giardia lamblia, entamoeba histolytica, and acanthamoeba spp. were studied by atomic force microscopy (afm). the preparation of fixed specimens was similar to methods used for either scanning or transmission electron microscopy. afm scanning was performed using both contact and tapping modes. a classical fixation procedure utilizing glutaraldehyde followed by ethanol dehydration w ...200011108031
characterization of a retrotransposon-like repetitive dna in entamoeba histolytica. 200011070310
[bacteriological quality of human drinking water in high-margination zones in chiapas].to analyze the bacteriological quality of drinking water (bqdw) and its association with diarrhoea and intestinal parasites in children 1 to 14 years old, in areas of high levels of poverty in chiapas, mexico.200011125624
mcm proteins of entamoeba histolytica. 200011070311
auxotrophy to lipoproteins of entamoeba histolytica cultivated under axenic conditions.entamoeba histolytica grows in media without serum but with a mixture of aminoacids, vitamins, lipoproteins, free cholesterol, phospholipids and fatty acids called pacsr. the ability of lipoproteins and free lipids to support growth of three e. histolytica strains (hk9, hmi:imss and hm3:imss) was analysed. tubes containing 5 ml culture medium, amino acids, vitamins and either 120-1,200 microg lipoproteins/ml or 0.017-0.10 mg free lipids/ml (predissolved in absolute ethanol) were inoculated with ...200011133106
serodiagnosis of amoebiasis using a recombinant protein fragment of the 29 kda surface antigen of entamoeba histolytica.to develop an improved serodiagnostic test for amoebiasis, we performed a detailed analysis of the immunodominant epitopes of the 29 kda surface antigen and evaluated its sensitivity and specificity. enzyme-linked immunosorbent assay (elisa) based on the fragment containing the immunodominant epitope was evaluated further and compared with full-length recombinant 29 kda protein. specificity and sensitivity of the two elisas were assessed using 55 human sera of parasitic protozoa infection cases ...200011428340
acute appendicitis caused by amebiasis.we report a case of appendicitis caused by amebiasis in a 45-year-old japanese man. he presented to our hospital with bloody stools in june 1998. sigmoidoscopy disclosed erosion, and a biopsy of the erosion showed colitis caused by entamoeba histolytica infection. four months later, he was admitted to our hospital with a small elastic mass and severe pain in the lower quadrant of the abdomen, which was diagnosed as acute appendicitis. he underwent appendectomy. histopathological examination reve ...200011085496
cyclospora infection in acquired immunodeficiency syndrome.diarrhea is a common clinical manifestation of human immunodeficiency virus (hiv) infection. the important protozoan pathogens causing diarrhea include cryptosporidium, microsporidium, isospora belli and cyclospora besides giardia lamblia and entamoeba histolytica.200011273176
presence of sequences homologous to the universal minicircle sequence (ums) of trypanosomatids in the dna circles of entamoeba histolytica. 200011070322
dna binding activity and predicted tertiary structure of the tata binding protein of entamoeba histolytica. 200011070323
the tail domain of entamoeba histolytica gef: a guanine nucleotide exchange factor. 200011070324
structural characterization of the 5'-flanking regions of the entamoeba histolytica ehcp112 and ehadh112 genes. 200011070325
isolation and identification of l10 gene from entamoeba histolytica homologous to wilms' tumor suppressor. 200011070326
immunogenicity of an optimized entamoeba histolytica gal-lectin dna vaccine. 200011070327
isolation of phage mimotopes mimicking a protective epitope of gpi-linked proteophosphoglycan antigens of entamoeba histolytica. 200011070328
bacterial expression of a human monoclonal antibody that inhibits in vitro adherence of entamoeba histolytica trophozoites. 200011070329
gastrointestinal parasites of mountain gorillas (gorilla gorilla beringei) in the parc national des volcans, rwanda.ninety-eight fecal samples were collected from 74 free-living mountain gorillas (gorilla gorilla beringei) from the parc national des volcans, rwanda, between july 1995 and january 1997 and examined for parasites by sheather's sugar and zinc sulfate flotation methods, trichrome staining, and larval cultures. all samples contained at least one parasite. seventeen endoparasites were identified, including eight protozoa, seven nematodes, one cestode, and one trematode. two species of arthropod mite ...200011237138
possible role of the ccaat/enhancer binding protein in the expression regulation of the ehpgp1 multidrug resistance gene in entamoeba histolytica. 200011070320
dna cloning of the entamoeba histolytica prp6 gene: a putative u4/u6 small nuclear ribonucleoprotein particle (snrnp). 200011070321
expression and immunodetection of a p-glycoprotein in emetine-resistant trophozoites of entamoeba histolytica. 200011070319
an initial characterization of the 3' untranslated region of the ehpgp5 mrna in entamoeba histolytica. 200011070317
expression of entamoeba histolytica ehpgp5 gene in xenopus laevis oocytes. 200011070318
current problems of amebiasis in japan and recent advances in amebiasis research.amebiasis is epidemic in two major populations in japan: male homosexuals and institutionalized people. currently available diagnostic, chemotherapeutic, and prophylactic measures and their problems are discussed. recent advances in basic research on amebiasis are also described with new findings of unique metabolisms and intracellular structures and organization. discoveries and analyses of the unique features presented by this parasitic protist help in our elucidation of the pathogenic mechani ...200011227020
secondary infections of aids autopsy cases in japan with special emphasis on mycobacterium avium-intracellulare complex infection.in order to study the frequency of secondary infections of aids autopsy cases in japan, especially the frequency of mycobacterium aviumintracellulare complex (mac) infection, retrospective autopsy study was conducted between 1986 and 1997 at the affiliated hospital of institute of medical sciences, university of tokyo. secondary infections of various organs from 43 aids autopsy cases were examined using histopathology, genetic diagnosis of tuberculosis, ziehl-neelsen stain for acid-fast bacilli ...200011211316
algorithm to predict minichef electrophoresis patterns of entamoeba histolytica dna. 200011070316
actin induction during pma and camp-dependent signal pathway activation in entamoeba histolytica trophozoites.activation of pkc or camp-dependent signalling pathways in entamoeba histolytica triggers the phosphorylation of proteins involved in actin rearrangements necessary for adhesion and locomotion. analogous motifs to sre and cre sequences--known to respond to pma and camp--were identified within the 5' regulatory region (5'rr) of one of the parasite actin genes. these sequences could be involved in the actin transcriptional upregulation reported during signalling. to test this hypothesis, a plasmid ...200011207595
caspase 3-dependent killing of host cells by the parasite entamoeba histolytica.the parasite entamoeba histolytica is named for its ability to lyse host tissues. to determine the factors responsible, we have initiated an examination of the contribution of parasite virulence factors and host caspases to cellular destruction by the parasite. amoebic colitis in c3h/hej mice was associated with extensive host apoptosis at sites of e. histolytica invasion. in vitro studies of e. histolytica-jurkat t-cell interactions demonstrated that apoptosis required contact via the amoebic g ...200011207613
socioeconomic status, clinical features, laboratory and parasitological findings of hepatic amebiasis patients--a hospital based prospective study in bangladesh.socioeconomic status, clinical, laboratory and parasitological features of 31 hospitalized amebic liver abscess (ala) and 8 amebic hepatitis (ah) patients were studied. thirty-seven (94.9%) of the total 39 cases were from low socioeconomic class and 2 (5.1%) were from middle class (p<0.001). sixteen (51.6%) ala and 5 (62.5%) ah patients were admitted with duration of disease for 3 weeks or more. twenty-one (67.7%) ala and 3 (37.5%) ah cases gave no previous history of diarrhea or dysentery. epig ...200011127347
the evolution of entamoeba, a cautionary tale.in addition to its medical importance, the parasite entamoeba histolytica has been studied by evolutionary biologists because of its unusual structural and biochemical features. recent molecular data have revealed that many of our assumptions regarding the relationships of the organism and its relatives have been incorrect.200011081575
comparison of antigen-capture elisa to stool-culture methods for the detection of asymptomatic entamoeba species infection in kafer daoud, egypt.we performed a prospective field study in the village of kafer daoud in menofia, egypt to compare the fecal culture method with enzyme linked immuno assay (elisa) for detection of 170 kda lectin antigen in feces for diagnosis of asymptomatic entamoeba histolytica and entamoeba dispar infection. all subjects with e. histolytica or e. dispar infection detected by culture also had positive elisa for amebic antigen in their feces and an additional 57 entameoba infections missed by culture were detec ...200011289667
two novel calcium-binding proteins from cytoplasmic granules of the protozoan parasite entamoeba histolytica.we report on the molecular characterisation of two novel granule proteins of the protozoon and human pathogen entamoeba histolytica. the proteins, which were named grainin 1 and 2, show a considerable structural similarity to calcium-binding proteins, particularly within ef-hand motifs. each grainin possesses three of these putative calcium-binding sites. based on careful inspection of known structures of protein families containing ef-hands, a domain of grainin 1 covering two ef-hand motifs was ...200011113449
intestinal parasites infestation and anemia in primary school children in gaza governorates--palestine.a comparative study was carried out to identify the prevalence of anemia, nutritional indices and intestinal parasitic infestation in primary school children. the target population included 209 pupils aged 6-11 years, attending schools in two areas, rimal area (urban) and jabalia village (rural), in gaza governorates. prevalence of intestinal parasites was high in jabalia village (more than 53%) in comparison to rimal area (33%). the main intestinal parasites were entamoeba histolytica, giardia ...200011845471
recent developments in amoebiasis:the gal/galnac lectins of entamoeba histolytica and entamoeba dispar.amoebiasis is responsible for 50000-100000 deaths annually. invasive amoebic disease begins with the attachment of entamoeba histolytica trophozoites to colonic mucin, a process mediated by the amoebic gal/galnac lectin. the non-pathogenic counterpart, e. dispar, is morphologically identical but genetically distinct. investigations comparing the gal/galnac lectin from these two organisms are under way.200011137050
trichomonas vaginalis: chromatin and mitotic spindle during mitosis.the mitotic phases and the changes that the chromatin and mitotic microtubules undergo during mitosis in the sexually transmitted parasite trichomonas vaginalis are described. parasites arrested in the gap 2 phase of the cell cycle by nutrient starvation were induced to mitosis by addition of fresh whole medium. [(3)h] thymidine labeling of trichomonad parasites for 24 h showed that parasites have at least four synchronic duplications after mitosis induction. fixed or live and acridine orange (a ...200011162363
recent developments in amoebiasis research.invasive amoebiasis, the infection of humans by entamoeba histolytica associated with dysentery and liver abscess, is still a major cause of morbidity and mortality in developing countries. this review attempts to reduce the gap between the overwhelming amount of information coming recently from laboratory research and the sparse contributions resulting from clinical and epidemiological investigations of the second parasitic cause of death resulting from a protozoan parasite.200011964813
development of a deltaglna balanced lethal plasmid system for expression of heterologous antigens by attenuated vaccine vector strains of vibrio cholerae.we have previously shown that more prominent immune responses are induced to antigens expressed from multicopy plasmids in live attenuated vaccine vector strains of vibrio cholerae than to antigens expressed from single-copy genes on the v. cholerae chromosome. here, we report the construction of a deltaglna derivative of v. cholerae vaccine strain peru2. this mutant strain, peru2deltaglna, is unable to grow on medium that does not contain glutamine; this growth deficiency is complemented by pke ...200010603391
innate immunity to amebic liver abscess is dependent on gamma interferon and nitric oxide in a murine model of disease.evidence from in vitro studies suggests that gamma interferon (ifn-gamma) and nitric oxide (no) are important in host defense against the protozoan parasite entamoeba histolytica. we used scid mice with targeted disruption of the ifn-gamma receptor gene and mice with targeted disruption of the gene encoding inducible no synthase to show that ifn-gamma plays a role in the innate immunity to amebic liver abscess seen in scid mice while no is required for control of amebic liver abscess in immunoco ...200010603416
granulysin: a novel antimicrobial peptide of cytolytic t lymphocytes and natural killer cells.granulysin is a novel antimicrobial protein produced by human cytolytic t lymphocytes and natural killer cells. it is active against a broad range of microbes, including gram-positive and gram-negative bacteria, fungi, and parasites. the fact that it kills mycobacterium tuberculosis is particularly important, since the current vaccine (bacille calmette-guerin, bcg) is of limited efficacy and antibiotic resistance is increasing. although functionally related to other antibacterial peptides, defen ...200010644038
fluorescence microscopy and fluorescence in situ hybridization of entamoeba histolytica nuclei to analyse mitosis and the localization of repetitive dna. 200010693751
two major 'higher molecular mass proteinases' of entamoeba histolytica are identified as cysteine proteinases 1 and 2. 200010693753
ectonucleotide diphosphohydrolase activities in entamoeba histolytica.in this work, we describe the ability of living cells of entamoeba histolytica to hydrolyze extracellular atp. in these intact parasites, whose viability was determined by motility and by the eosin method, atp hydrolysis was low in the absence of any divalent metal (78 nmol p(i)/h/10(5) cells). interestingly, in the presence of 5 mm mgcl(2) an ecto-atpase activity of 300 nmol p(i)/h/10(5) cells was observed. the addition of mgcl(2) to the extracellular medium increased the ecto-atpase activity i ...200010700386
[gastrointestinal diseases associated with hiv infection].a clinical studies were carried out on gastrointestinal diseases associated with hiv infection. during the 6 years between january 1993 and december 1998, 71 hiv infected cases visited to yokohama municipal citizen's hospital, and 26 of them developed gastrointestinal complications during the course of their illness. they consisted of 24 males and 2 females, with the mean age of 44.7 years and the medial value of 42.5 years. of the 26 patients, 21 were japanese, and the remaining 5 were southeas ...200010695296
a subunit vaccine candidate region of the entamoeba histolytica galactose-adherence lectin promotes interleukin-12 gene transcription and protein production in human macrophages.the cysteine-rich region of the 170-kda subunit galactose-adherence lectin (gal-lectin) of entamoeba histolytica is a subunit vaccine candidate and a protective antigen in the gerbil model of amebiasis. macrophage-mediated immunity is important for protection against e. histolytica and is activated by th1 cytokines. as th1 differentiation is promoted by il-12, we investigated what portion of the gal-lectin could stimulate il-12 in human thp-1 macrophages. native gal-lactin stimulated il-12 p40 / ...200010671197
extracellular matrix-induced signaling in entamoeba histolytica: its role in invasiveness.entamoeba histolytica trophozoites can invade the mucosa of the host large intestine to cause disease. here, isaura meza describes recent work indicating that the trophozoites respond to the binding of extracellular matrix proteins by activating receptor-mediated signal transduction pathways. activation of phosphokinase c or adenylyl cyclase induces protein phosphorylation, actin gene expression and reorganization of the actin cytoskeleton to form adhesive structures that promote interaction wit ...200010637584
compositional pressure and translational selection determine codon usage in the extremely gc-poor unicellular eukaryote entamoeba histolytica.it is widely accepted that the compositional pressure is the only factor shaping codon usage in unicellular species displaying extremely biased genomic compositions. this seems to be the case in the prokaryotes mycoplasma capricolum, rickettsia prowasekii and borrelia burgdorferi (gc-poor), and in micrococcus luteus (gc-rich). however, in the gc-poor unicellular eukaryotes dictyostelium discoideum and plasmodium falciparum, there is evidence that selection, acting at the level of translation, in ...200010721724
partial characterization of a 36-kda antigen of entamoeba histolytica and its recognition by sera from patients with amoebiasis.a 36-kda antigen of axenically grown pathogenic entamoeba histolytica (hm1-imss) was eluted from the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page)-resolved crude amoebic extract antigens. the immunoreactivity of this partially purified 36-kda antigen with monoclonal antibody (moab) 3d(10) altered significantly (p<0.01) after heat and trypsin treatment but remained unaltered after treatment with sodium metaperiodate (p0.5), thereby indicating the protein nature of the epito ...200010617786
absence of lipophosphoglycan-like glycoconjugates in entamoeba dispar.invasive amoebiasis is the result of infection of entamoeba histolytica. the closely related entamoeba dispar can colonize the human gut but does not cause invasive disease. in this study, e. dispar was analysed for the presence of the lipophosphoglycan-like (lpg) glycoconjugate known to be present on the cell surface of e. histolytica. e. dispar cells were radio-isotope labelled with [3h]galactose or [3h]inositol. the acidic glycoconjugates were extracted and analysed by hydrophobic chromatogra ...200010726263
differences in substrate specificity and kinetic properties of the recombinant hexokinases hxk1 and hxk2 from entamoeba histolytica.entamoeba histolytica is responsible for amoebic colitis and liver abscess in humans. entamoeba dispar is a closely related, morphologically indistinguishable nonpathogenic species. the hexokinase (atp:d-hexose 6-phosphotransferase, ec 2.7.1.1) isoenzyme patterns distinguish the pathogenic and nonpathogenic species. both species possess two hexokinases with very similar molecular mass and different isoelectric points. in order to understand the role of the two different isoenzymes from e. histol ...200010613700
asymptomatic cyst passers of entamoeba histolytica but not entamoeba dispar in institutions for the mentally retarded in japan.entamoeba histolytica/entamoeba dispar was isolated from 50 asymptomatic amebic cyst passers in three institutions for the mentally retarded in kanagawa prefecture, japan. to distinguish between e. histolytica and e. dispar, the isolates were analyzed by pcr, reactivity to monoclonal antibodies, and zymodemes. all isolates were identified as e. histolytica. the results lead us to conceive that, in japan, e. histolytica is predominant even in asymptomatic cyst passers.200010729715
3'-deoxyribonucleosides and their derivatives as anti-amoebic agents.in general nucleoside analogues were found to possess in vitro amoebicidal property against trophozoites of entamoeba histolytica. the acid-labile nature of these compounds completely destroyed their ability to cure caecal amoebiasis of rats. however the therapeutic efficacy of one of these compounds yielded most promising results, with 10% cures when it was administered as enteric coated formulation.200010601681
cysteine proteinases and the pathogenesis of amebiasis.amebiasis is a major cause of morbidity and mortality throughout the tropical world. entamoeba histolytica is now recognized as a separate species from the morphologically identical e. dispar, which cannot invade. cysteine proteinases are a key virulence factor of e. histolytica and play a role in intestinal invasion by degrading the extracellular matrix and circumventing the host immune response through cleavage of secretory immunoglobulin a (siga), igg, and activation of complement. cysteine p ...200010755997
pathogenesis of intestinal amebiasis: from molecules to disease.in spite of a wealth of knowledge on the biochemistry and cellular and molecular biology of entamoeba histolytica, little has been done to apply these advances to our understanding of the lesions observed in patients with intestinal amebiasis. in this review, the pathological and histological findings in acute amebic colitis are related to the molecular mechanisms of e. histolytica pathogenicity described to date. infection of the human colon by e. histolytica produces focal ulceration of the in ...200010756002
evidence that hyaluronidase is not involved in tissue invasion of the protozoan parasite entamoeba histolytica.as previous reports suggested that a hyaluronidase is involved in tissue invasion of entamoeba histolytica, we searched for such an activity in trophozoite extracts. a hyaluronidase activity was not detectable in long-term cultures or in amoebae freshly passaged through a gerbil liver, as evidenced by four different techniques.200010769014
antiamoebic and spasmolytic activities of extracts from some antidiarrhoeal traditional preparations used in kinshasa, congo.three major extracts from some traditional preparations, based on medicinal plants, used as antidiarrhoeal agents were investigated for their putative antiamoebic and spasmolytic activities in vitro. results indicated that both biological activities are concentrated in the polyphenolic fraction, and not in the saponin or alkaloid containing fractions. the most active polyphenolic extracts were those from euphorbia hirta whole plant, leaves of alchornea cordifolia, crossopteryx febrifuga, nauclea ...200010782488
isolation of emetine resistant clones of entamoeba histolytica by petri dish agar method.emetine resistant clones of entamoeba histolytica strain hm1:imss were isolated by using petri dish agar method after mutation with ethyl-methanesulphonate. two emetine resistant clones were obtained and both were resistant to emetine at a concentration of 24 micrograms/ml of emetine. the 50 per cent inhibitory concentration (ic50) for both emetine sensitive and resistant clones was 5 and 14 micrograms/ml respectively. the colony forming efficiency of e. histolytica strain hm1:imss varied from 4 ...200010793488
an entamoeba histolytica inositol 1,3,4-trisphosphate 5/6-kinase has a novel 3-kinase activity. 200010802324
vacuolar localization of an entamoeba histolytica homologue of the plasma membrane atpase (pmca). 200010802325
cell killing by the human parasite entamoeba histolytica is inhibited by the rho-inactivating c3 exoenzyme. 200010802329
inguinal lymphadenitis caused by entamoeba histolytica: case report and literature review.liver abscesses are the most common manifestation of extraintestinal infection by entamoeba histolytica. involvement of other sites, including the peritoneum, pericardium, brain, or genitourinary tract, is unusual. we describe a case of inguinal necrotizing lymphadenitis caused by e histolytica. our patient responded well to surgical drainage, metronidazole, and paramomycin therapy. a literature review of genitourinary and other uncommon sites of e histolytica infection is included.200010807081
enteropathogens in adult patients with diarrhea and healthy control subjects: a 1-year prospective study in a swedish clinic for infectious diseases.a 1-year prospective study was conducted to identify enteropathogens in adults with diarrhea (n=851) and in healthy control subjects (n=203) by use of conventional laboratory methods. virulence factor genes for diarrheagenic escherichia coli were detected by polymerase chain reaction. enteropathogens were identified in 56% of patients and 16% of control subjects. the isolation rate was 65% for patients with symptoms for <1 week and for travelers; >1 pathogen was found in 11% of patients. the mos ...200010816147
a novel protein kinase from brassica juncea stimulated by a protozoan calcium binding protein. purification and partial characterization.a novel protein kinase (bjccabpk) from etiolated brassica juncea seedlings has been purified and partially characterized. the purified enzyme migrated on sds/page as a single band with an apparent molecular mass of 43 kda. the optimum ph for the kinase activity was 8.0. it was stimulated more than sixfold by the protozoa entamoeba histolytica calcium binding protein ehcabp (10.5 nm) but not by calmodulin (cam) when used at equimolar concentration. moreover the kinase also did not bind cam-sephar ...200010824102
diagnosis of invasive amebiasis by enzyme-linked immunosorbent assay of saliva to detect amebic lectin antigen and anti-lectin immunoglobulin g antibodies.saliva from subjects with amebic liver abscess (ala), acute amebic colitis, asymptomatic infection with entamoeba histolytica or entamoeba dispar, and uninfected controls was tested by enzyme-linked immunosorbent assay (elisa) for the presence of e. histolytica galactose-inhibitable lectin antigen and salivary immunoglobulin (igg) antibodies to a recombinant cysteine-rich lectin-derived protein (lc3). salivary lectin antigen was found in 65.8% of subjects with acute colitis, compared to 22.2% of ...200010835000
inter- and intra-strain variation in the 5.8s ribosomal rna and internal transcribed spacer sequences of entamoeba histolytica and comparison with entamoeba dispar, entamoeba moshkovskii and entamoeba invadens.the ribosomal rna genes in entamoeba histolytica are located on circular dna molecules in about 200 copies per genome equivalent. nucleotide sequence analysis of the 5.8s rrna gene and the flanking internal transcribed spacers was carried out to determine the degree of sequence divergence in the multiple rrna gene copies of a given strain; amongst three different e. histolytica strains (hm-1:imss, rahman and hk-9); and amongst four species of entamoeba (entamoeba histolytica, entamoeba dispar, e ...200010856506
the entamoeba histolytica mitochondrion-derived organelle (crypton) contains double-stranded dna and appears to be bound by a double membrane.amebae have an hsp60-associated, mitochondrion-derived organelle (crypton). in this study, the crypton was stained with multiple dna-binding fluorochromes and a monoclonal anti-double-stranded dna antibody. transmission microscopy of partially purified cryptons revealed organelles bound by a double membrane.200010858251
pcr detection of giardia lamblia in stool: targeting intergenic spacer region of multicopy rrna gene.a pcr based detection that amplifies the 552-bp intergenic spacer (igs) region of multicopy rrna gene of giardia lamblia and 320-bp internal sequences to first pcr product has been used in diagnosis of giardiasis in stool sample. the primers were found highly specific to giardia spp. only, because no amplification was observed with dnas from other enteric pathogens like escherichia coli, shigella dysenteriae and entamoeba histolytica. the test could detect even less than 2 pg of genomic dna from ...200010860716
evaluation of indirect fluorescent antibody test and enzyme-linked immunosorbent assay for diagnosis of hepatic amebiasis in bangladesh.serum samples of 31 amebic liver abscess (ala) patients, 8 amebic hepatitis (ah) patients, and 60 controls were tested for anti-amebic igg by enzyme-linked immunosorbent assay (elisa) and indirect fluorescent antibody tests (ifat). sera of 29 (93.6%) ala and 6 (75%) ah patients and 2 (3.3%) control subjects were positive by ifat. anti-amebic antibody titer above the cutoff point (= 0.168; x + 2 sd of control sera) was observed in sera of 27 (87%) ala, 4 (50%) ah, and 1 (1.7%) control by elisa. a ...200010864262
inhibition and stimulation of growth of entamoeba histolytica in culture: association with pkc activity and protein phosphorylation.we studied the role of protein kinase c (pkc) and protein threonine phosphorylation in the inhibition and stimulation of growth of the protozoan parasite entamoeba histolytica. pkc was activated after serum deprivation in e. histolytica and during this period proteins became threonine phosphorylated. conversely, on serum stimulation of serum-deprived cells, pkc activation was rapidly reversed and the threonine phosphorylation of proteins quickly declined. growth of e. histolytica was not affecte ...200010864515
bacterial, viral and parasitic enteric pathogens associated with acute diarrhea in hospitalized children from northern jordan.to determine the etiology of acute diarrhea in jordanian children under 5 years of age, we examined stool samples from 265 children admitted to the pediatric ward at princess rahma hospital for children, irbid, jordan, for parasites, rotavirus and enteric bacteria. using both traditional and molecular diagnostic techniques, we detected enteropathogens in 66.4% of patients with diarrhea. a single enteric pathogen was detected in 50.9% of the children, and multiple pathogens were detected in 15.5% ...200010865179
[amebiasis].amoebiasis caused by the enteric protozoan entamoeba histolytica is a widespread parasitic disease, which causes 40,000 to 100,000 deaths per year. cases diagnosed in denmark are always imported. infection results from ingestion of amoebic cysts, which after excystation form trophozoits in the small intestine, colonize the bowel lumen and invade the intestinal epithelium resulting in amoebic colitis. spread to the liver and formation of amoebic liver abscesses occurs in one third of the cases, w ...200010868107
osteomyelitis of the ribs in the antibiotic era.a total of 106 cases of rib osteomyelitis were reviewed, including 2 cases described in detail. mycobacterial and bacterial infections accounted for 47 cases each. fungal rib osteomyelitis occurred in 11 cases and 1 case was caused by entamoeba histolytica. most cases occurred in children and young adults. the mean duration of symptoms before diagnosis was 16, 26 and 32 weeks for bacterial, mycobacterial and fungal rib osteomyelitis, respectively. common clinical signs were fever (73%), soft tis ...200010879590
rapd in the analysis of isolates of entamoeba histolytica.genetic variability in entamoeba histolytica was analyzed by random amplified polymorphic dna (rapd) using ten arbitrary primers. due to intrinsic characteristics of the rapd technique only axenic samples were analyzed. since the presence of any microorganism in the cultures interfered in the dna profile by generating rapds not pertaining to e. histolytica. the rapd profiles of e. histolytica samples isolated from patients with different clinical manifestations from different regions of the amer ...200010708008
cell polarization and adhesion in a motile pathogenic protozoan: role and fate of the entamoeba histolytica gal/galnac lectin.the human pathogenic protozoan entamoeba histolytica is a motile cell polarized into a front pseudopod and a rear uroid. the amoebic gal/galnac surface lectin is a major adhesion molecule composed of an immunodominant 170-kda heavy subunit, mostly extracellular except for a short cytoplasmic tail, and of an extracellular light subunit. the binding of multivalent ligands triggers lectin capping and recruitment to the uroid. the properties of the gal/galnac lectin and its role in amoeba adhesion a ...200010884615
epidemiology of infections with intestinal parasites and human immunodeficiency virus (hiv) among sugar-estate residents in ethiopia.intestinal parasitic infections could play an important role in the progression of infection with human immunodeficiency virus (hiv), by further disturbing the immune system whilst it is already engaged in the fight against hiv. hiv and intestinal parasitic infections were investigated in 1239, randomly selected individuals, aged 15-54 years, living on a sugar estate in central ethiopia. intestinal parasites were identified in faecal samples (one/subject) using direct, concentration, and (for st ...200010884872
the major surface antigens of entamoeba histolytica trophozoites are gpi-anchored proteophosphoglycans.trophozoites of the parasitic protozoa, entamoeba histolytica, synthesize a cell surface lipoglycoconjugate, termed lipophosphoglycan, which is thought to be an important virulence factor and potential vaccine candidate against invasive amebiasis. here, we show that the e. histolytica lipophosphoglycans are in fact glycosylphosphatidylinositol (gpi)-anchored proteophosphoglycans (ppgs). these ppgs contain a highly acidic polypeptide component which is rich in asp, glu and phosphoserine residues. ...200010715210
enolase from trypanosoma brucei, from the amitochondriate protist mastigamoeba balamuthi, and from the chloroplast and cytosol of euglena gracilis: pieces in the evolutionary puzzle of the eukaryotic glycolytic pathway.genomic or cdna clones for the glycolytic enzyme enolase were isolated from the amitochondriate pelobiont mastigamoeba balamuthi, from the kinetoplastid trypanosoma brucei, and from the euglenid euglena gracilis. clones for the cytosolic enzyme were found in all three organisms, whereas euglena was found to also express mrna for a second isoenzyme that possesses a putative n-terminal plastid-targeting peptide and is probably targeted to the chloroplast. database searching revealed that arabidops ...200010889212
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