Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| nonculture-based identification of bacteria in milk by protein fingerprinting. | traditional methods for bacterial identification include gram staining, culturing, and biochemical assays for phenotypic characterization of the causative organism. these methods can be time-consuming because they require in vitro cultivation of the microorganisms. recently, however, it has become possible to obtain chemical profiles for lipids, peptides, and proteins that are present in an intact organism, particularly now that new developments have been made for the efficient ionization of bio ... | 2012 | 22807025 |
| molecular basis for different levels of tet(m) expression in streptococcus pneumoniae clinical isolates. | seventy-four unrelated clinical isolates of streptococcus pneumoniae harboring the tet(m) gene were studied. seven strains with low tetracycline (tc) mics (0.25 to 0.5 μg/ml) were found to harbor truncated tet(m) alleles that were inactivated by different frameshift mutations. in contrast, five strains bore deletions in the tet(m) promoter region, among which four displayed increased tc mics (16 to 64 μg/ml). the same promoter mutations were detected in tc-resistant mutants selected in vitro fro ... | 2012 | 22802249 |
| in vitro activity of ceftaroline and comparator antimicrobials against european and middle east isolates from complicated skin and skin-structure infections collected in 2008-2009. | the activities of ceftaroline, the active metabolite of the pro-drug ceftaroline fosamil, a novel anti-meticillin-resistant staphylococcal cephalosporin, and nine comparators were determined against surveillance isolates collected in 2008-2009. over 3000 isolates associated with complicated skin and skin-structure infections (csssis) were collected from 106 centres in 19 countries. mics were determined using clsi broth microdilution methodology. clonal relatedness of meticillin-resistant staphyl ... | 2012 | 22796201 |
| use of multiparameter flow cytometry to determine the effects of monoterpenoids and phenylpropanoids on membrane polarity and permeability in staphylococci and enterococci. | monoterpenoids and phenylpropanoids are major components of many plant essential oils and are relatively simple, low-molecular-weight compounds with antimicrobial activity. this study used multiparameter flow cytometry to examine changes in membrane polarity and permeability in staphylococcus aureus, staphylococcus epidermidis and enterococcus faecalis following exposure to the monoterpenoids carvacrol, 1,8-cineole and terpinen-4-ol and the phenylpropanoids eugenol and cinnamaldehyde. melaleuca ... | 2012 | 22795655 |
| efficacy of berberine, an antimicrobial plant alkaloid, as an endodontic irrigant against a mixed-culture biofilm in an in vitro tooth model. | berberine, a plant alkaloid isolated from many medicinal plants, has shown antimicrobial activity against selected oral pathogens. the purpose of this investigation was to evaluate the antimicrobial efficacy of berberine solution against selected endodontic pathogens using a multispecies biofilm tooth model. | 2012 | 22794217 |
| evidence for occurrence, persistence, and growth potential of escherichia coli and enterococci in hawaii's soil environments. | high densities of escherichia coli and enterococci are common in freshwaters on oahu and other hawaiian islands. soil along stream banks has long been suspected as the likely source of these bacteria; however, the extent of their occurrence and distribution in a wide range of soils remained unknown until the current investigation. soil samples representing the seven major soil associations were collected on the island of oahu and analyzed for fecal coliforms, e. coli, and enterococci by the most ... | 2012 | 22791049 |
| the pava-like fibronectin-binding protein of enterococcus faecalis, efba, is important for virulence in a mouse model of ascending urinary tract infection. | enterococcus faecalis is an established nosocomial pathogen, yet the pathogenesis of enterococcal infections, particularly of urinary tract infections (utis), remains to be fully elucidated. fibronectin-binding proteins have been identified as potent adhesins in pathogenic gram-positive cocci. here, we characterized efba, which is encoded by the enterococcal orthologue of streptococcus pneumoniae pava. similar to pava, the anchorless efba protein was localized to the enterococcal cell outer surf ... | 2012 | 22782954 |
| volatile organic compound analysis by ion molecule reaction mass spectrometry for gram-positive bacteria differentiation. | approximately 50 % of all clinically proven infections in critically ill patients are caused by gram-positive bacteria. the timely and appropriate treatment of these infections is vital in order to avoid negative outcomes. hence, fast and reliable methods are needed for the early detection and identification of microorganisms. recently, direct mass spectrometry-based analysis of volatile organic compounds emitted by microorganisms has been employed to study gram-negative bacteria. here, we repor ... | 2012 | 22782437 |
| infected total knee arthroplasty treatment outcome analysis. | infected total knee arthroplasty (tka) is a topic of great importance, because its diagnosing and treatment requires a lot of resources, and often has an unsatisfactory outcome. the aim of this study was to analyze the outcome of the treatment of infection developed following tka. | 2012 | 22779296 |
| characterization of a conjugate between rose bengal and chitosan for targeted antibiofilm and tissue stabilization effects as a potential treatment of infected dentin. | bacterial biofilms and dentin structural changes are some of the major challenges in the management of infected dentin tissue. this study characterized a photosensitizer-conjugated chitosan with enhanced photodynamic efficacy against dental biofilms, as well as the ability to reinforce the postinfected dentin matrix in order to improve its mechanical and chemical stability. rose bengal-conjugated chitosan (csrb) was synthesized using a chemical cross-linking method and characterized for photophy ... | 2012 | 22777042 |
| complete genome sequence of enterococcus faecium strain tx16 and comparative genomic analysis of enterococcus faecium genomes. | enterococci are among the leading causes of hospital-acquired infections in the united states and europe, with enterococcus faecalis and enterococcus faecium being the two most common species isolated from enterococcal infections. in the last decade, the proportion of enterococcal infections caused by e. faecium has steadily increased compared to other enterococcus species. although the underlying mechanism for the gradual replacement of e. faecalis by e. faecium in the hospital environment is n ... | 2012 | 22769602 |
| enterococcus faecium stimulates human neutrophils via the formyl-peptide receptor 2. | the human formyl-peptide receptor 2 (fpr2/alx) senses phenol-soluble modulin (psm) peptide toxins produced by pathogenic staphylococcal species and plays a crucial role in directing neutrophil influx during staphylococcal infection. however, it has remained unclear if fpr2 responds also to molecules from other bacterial pathogens. here we analyzed a variety of gram-positive and gram-negative pathogens and found that apart from staphylococci only certain enterococcal strains have the capacity to ... | 2012 | 22768166 |
| nosocomial infections in a pediatric intensive care unit of a developing country: nhsn surveillance. | this study aimed to determine the epidemiology of the three most common nosocomial infections (ni), namely, sepsis, pneumonia, and urinary tract infection (uti), in a pediatric intensive care unit (picu) in a developing country and to define the risk factors associated with ni. | 2012 | 22767099 |
| aseptic simulation test challenged with microorganisms for validation of pharmacy operators. | aseptic technique of pharmacy operators was assessed using simulated media-fill tests challenged with microorganisms. | 2012 | 22761076 |
| type i and type ii mechanisms of antimicrobial photodynamic therapy: an in vitro study on gram-negative and gram-positive bacteria. | antimicrobial photodynamic therapy (apdt) employs a non-toxic photosensitizer (ps) and visible light, which in the presence of oxygen produce reactive oxygen species (ros), such as singlet oxygen ((1) o(2), produced via type ii mechanism) and hydroxyl radical (ho(.), produced via type i mechanism). this study examined the relative contributions of (1) o(2) and ho(.) to apdt killing of gram-positive and gram-negative bacteria. | 2012 | 22760848 |
| in vitro activities of antimicrobial cationic peptides; melittin and nisin, alone or in combination with antibiotics against gram-positive bacteria. | the in vitro activities of two antimicrobial cationic peptides, melittin and nisin alone and in combination with frequently used antibiotics (daptomycin, vancomycin, linezolid, ampicillin, and erythromycin), were assessed against clinical isolates of methicillin-susceptible staphylococcus aureus, methicillin-resistant s. aureus and enterococcus faecalis. using the broth microdilution method, minimum inhibitory concentration (mic) ranges of melittin and nisin against all strains were 2-8 μg/ml an ... | 2012 | 22759757 |
| antimicrobial resistance among gram-positive pathogens in saudi arabia. | several species of gram-positive cocci are major nosocomial or community pathogens associated with morbidity and mortality. here, we review the antimicrobial resistance among these pathogens in saudi arabia. in the last decades, antimicrobial resistance has increased among staphylococcus aureus in the kingdom with a growing prevalence of both nosocomial and community methicillin-resistant s. aureus (mrsa) isolates. as yet, no vancomycin-resistant mrsa have been reported, although isolates with r ... | 2012 | 22759756 |
| identification, structural, and biochemical characterization of a group of large csn2 proteins involved in crispr-mediated bacterial immunity. | many prokaryotic organisms acquire immunity against foreign genetic material by incorporating a short segment of foreign dna called spacer into chromosomal loci, termed clustered regularly interspaced short palindromic repeats (crisprs). the encoded rnas are processed into small fragments that guide the silencing of the invading genetic elements. the crispr-associated (cas) proteins are the main executioners of these processes. herein, we report the crystal structure of stu0660 of streptococcus ... | 2012 | 22753072 |
| mechanisms for photoinactivation of enterococcus faecalis in seawater. | field studies in fresh and marine waters consistently show diel fluctuations in concentrations of enterococci, indicators of water quality. we investigated sunlight inactivation of enterococcus faecalis to gain insight into photoinactivation mechanisms and cellular responses to photostress. e. faecalis bacteria were exposed to natural sunlight in clear, filtered seawater under both oxic and anoxic conditions to test the relative importance of oxygen-mediated and non-oxygen-mediated photoinactiva ... | 2012 | 22941072 |
| non-tuberculous mycobacteria and their surface lipids efficiently induced il-17 production in human t cells. | interleukin-17 (il-17) is produced by a subset of cd4(+) t helper (th) lymphocytes known as th17 cells. in humans, il-1β, enhanced by il-6 and il-23 is crucial for differentiation of these cells. il-17 evokes inflammation and is involved in host defence against microorganisms, although little is known about its role in diseases caused by non-tuberculous mycobacteria. the genus mycobacterium contains both obligate and opportunistic pathogens as well as saprophytes, and the mycobacterial cell enve ... | 2012 | 22939918 |
| genome sequence of enterococcus hirae (streptococcus faecalis) atcc 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis. | enterococcus hirae atcc 9790 is a gram-positive lactic acid bacterium that has been used in basic research for over 4 decades. here we report the sequence and annotation of the 2.8-mb genome of e. hirae and its endemic 29-kb plasmid ptg9790. | 2012 | 22933757 |
| enterococcus septicemia due to a pacemaker lead infection. | pacemaker lead infections are rare but potentially life threatening. we report the case of an 80-year-old pacemaker-dependent female patient with complete heart block, who presented with subfebrile temperatures and chills due to a pacemaker lead infection by enterococcus faecalis. | 2012 | 22929569 |
| antibacterial activity of a plant extract and its potential for disinfecting gutta-percha cones. | this study evaluated the antibacterial activity of rosmarinus officinalis extract and its potential for disinfecting guttapercha (gp) cones. in the first experiment, a hydro-alcoholic extract of rosmarinus officinalis (leaves) in a dilution ratio of 10% m/v was tested against enterococcus faecalis by using the disk diffusion method. positive and negative controls were 70% cereal alcohol and antibiotics, respectively. the procedures were performed in triplicate, and the diameters of the zones of ... | 2012 | 22928375 |
| enterococcus faecalis: a biological marker predicting the emergence of necrotizing enterocolitis. | 2012 | 22924710 | |
| antibacterial efficacy of casein-derived peptides against enterococcus faecalis. | the aim of this study was to test a casein peptide in its glycosylated form (kappa-casein glycopeptide, kcgp) and its non-glycosylated form (kappa-casein peptide, kcp) for antibacterial efficacy against enterococcus faecalis in planktonic and biofilm cultures. | 2012 | 22924358 |
| tnf-α mediates macrophage-induced bystander effects through netrin-1. | macrophage-induced bystander effects have been implicated as an important mediator of chromosomal instability and colon cancer triggered by enterococcus faecalis, a human intestinal commensal bacteria. there is little understanding about how inflammatory cytokines mediate bystander effects, but questions in this area are important because of the pivotal contributions made by inflammatory processes to cancer initiation and progression. here, we report that the central proinflammatory cytokine tnf ... | 2012 | 22915753 |
| secondary cell wall polymers of enterococcus faecalis are critical for resistance to complement activation via mannose-binding lectin. | the complement system is part of our first line of defense against invading pathogens. the strategies used by enterococcus faecalis to evade recognition by human complement are incompletely understood. in this study, we identified an insertional mutant of the wall teichoic acid (wta) synthesis gene tagb in e. faecalis v583 that exhibited an increased susceptibility to complement-mediated killing by neutrophils. further analysis revealed that increased killing of the mutant was due to a higher ra ... | 2012 | 22908219 |
| increased pheromone ccf10 expression in enterococcus faecalis biofilm formed by isolates from renal transplant patients. | renal transplant recipients are at a high risk of developing infectious complications even caused by commensal bacteria. this is because of various physiological non-immunological, and immunological protective mechanisms are not fully efficient in rtx patients. therefore, rapid and precise diagnostic tools are essential in this particular group of patients. we aimed to develop simple and sensitive protocol flow-fish for the study of gene expression in enterococci and to compare expression of gen ... | 2012 | 22907591 |
| characterization and risk factors of vancomycin-resistant enterococci (vre) among animal-affiliated workers in malaysia. | this study determined the risk factors and characteristics of vancomycin-resistant enterococci (vre) among individuals working with animals in malaysia. | 2012 | 22906187 |
| [daptomycin in the treatment of infectious endocarditis]. | overcoming polyresistance of pathogens of infectious endocarditis (ie) is only possible through the use of new antibiotics, such as daptomycin, that was successfully used in 4 patients. the clinical features of endocarditis caused by enterococcus faecalis resistant to penicillin, gentamicin, and streptomycin are described. cases of ie with negative blood cultures and a failure of previous antibacterial therapy are considered. the literature data on the use of daptomycin in ie caused by oxacillin ... | 2012 | 22896985 |
| ampicillin susceptibility can predict in vitro susceptibility of penicillin-resistant, ampicillin-susceptible enterococcus faecalis isolates to amoxicillin but not to imipenem and piperacillin. | our findings demonstrated that the results obtained for ampicillin may accurately predict the in vitro susceptibility to amoxicillin but not to imipenem and piperacillin among isolates of enterococcus faecalis resistant to penicillin but susceptible to ampicillin, which have emerged recently, in contrast to penicillin- and ampicillin-susceptible isolates. | 2012 | 22895041 |
| the effect of tissue inhibitors on the antibacterial activity of chitosan nanoparticles and photodynamic therapy. | newer antibacterial alternatives such as chitosan nanoparticles (csnps) and photodynamic therapy (pdt) have been investigated to achieve effective root canal disinfection. the current study aims to assess the effect of various tissue inhibitors such as dentin, dentin matrix, pulp tissue, bacterial lipopolysaccharides (lpss), and bovine serum albumin (bsa) on the antibacterial activity of csnps and pdt. | 2012 | 22892750 |
| microbial biofilm proliferation within sealer-root dentin interfaces is affected by sealer type and aging period. | root canal fillings are intended to prevent microbial proliferation over time in the canal after treatment. the objective of this study was to assess biofilm proliferation within the sealer-dentin interfaces of 2 methacrylate resin-based systems, self-etch (se) and total-etch (te), and an epoxy resin-based sealer (ep), aged for up to 6 months. | 2012 | 22892745 |
| an assessment of antibacterial activity of four endodontic sealers on enterococcus faecalis by a direct contact test: an in vitro study. | aim. to evaluate the antibacterial activity of four endodontic sealers on enterococcus faecalis by a direct contact test. material and methods. enterococcus faecalis was used as a test organism. direct contact test which is based on measuring the effect of close contact between test bacteria and tested material on the kinetics of bacterial growth was performed to overcome the disadvantages of agar diffusion test. the sealers tested were zinc oxide eugenol-based sealer, glass-ionomer-based sealer ... | 2012 | 22888444 |
| complete genome sequence of bacteriophage bc-611 specifically infecting enterococcus faecalis strain np-10011. | enterococcus faecalis is an opportunistic pathogen that causes serious infections in humans and animals and is also an important bacterium for dairy and probiotic supplement production. therefore, bacteriophages infecting e. faecalis may be useful for phage therapy against multidrug-resistant strains or may threaten industrial fermentation. we isolated a virulent siphoviridae bacteriophage, bc-611, specifically infecting e. faecalis strain np-10011 but not infecting other e. faecalis strains or ... | 2012 | 22879611 |
| aerobic glycerol dissimilation via the enterococcus faecalis dhak pathway depends on nadh oxidase and a phosphotransfer reaction from pep to dhak via eiiadha. | two pathways for glycerol dissimilation are present in enterococcus faecalis. either glycerol is first phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase with molecular oxygen as the electron acceptor (glpo/glpk pathway), or it is first oxidized by glycerol dehydrogenase with nad(+) as the acceptor of the reduction equivalents and then phosphorylated by dihydroxyacetone kinase (glda/dhak pathway). the final end product in both cases is dihydroxyacetone phosphate ... | 2012 | 22878395 |
| comparative evaluation of the antimicrobial activity of natural extracts of morinda citrifolia, papain and aloe vera (all in gel formulation), 2% chlorhexidine gel and calcium hydroxide, against enterococcus faecalis: an in vitro study. | a comparative evaluation of the antimicrobial activity of natural extracts of morinda citrifolia, papain, and aloe vera (all in gel formulations), 2% chlorhexidine gel and calcium hydroxide, against enterococcus faecalis-an in vitro study. | 2012 | 22876022 |
| effect of freezing on photoreactivation of escherichia coli and enterococcus faecalis. | the effect of freezing on photoreactivation of two strains of escherichia coli (atcc strain 25922 and o157:h7 strain 961019) and two strains of enterococcus faecalis (strain atcc 51299, vancomycin-resistant and strain atcc 29212, vancomycin-sensitive) following ultraviolet irradiation were examined. the level of log photoreactivation of the freezing treated test organisms (frozen at -7, -15, or -30 degrees c then thawed at room temperature prior to ultraviolet irradiation) was compared with that ... | 2012 | 22866394 |
| characterization of antimicrobial resistance and virulence genotypes of enterococcus faecalis recovered from a pork processing plant. | the objective of this study was to assess the antimicrobial resistance and virulence genotypes of enterococcus faecalis isolated from samples obtained from a commercial pork processing plant. a total of 200 samples were randomly obtained from carcasses after bleeding (bc; 50 samples) and pasteurization (pc; 100 samples) and from retail pork products (rp; 50 samples). one isolate from each e. faecalis -positive sample was analyzed for antimicrobial susceptibility and characterized using a enteroc ... | 2012 | 22856575 |
| luxs-dependent ai-2 regulates versatile functions in enterococcus faecalis v583. | bacteria utilize a quorum sensing (qs) system to coordinate gene expression by monitoring the concentration of molecules known as autoinducers (ai). in the present study, we confirmed the presence of a luxs/ai-2 dependent qs system in vancomycin-resistant enterococcus faecalis v583. then, the cellular targets controlled by ai-2 were identified by comparative proteomics analysis in order to elucidate the possible role of ai-2 in e. faecalis. results demonstrated 15 proteins that are differentiall ... | 2012 | 22856334 |
| stimulation of cadaverine production by foodborne pathogens in the presence of lactobacillus, lactococcus, and streptococcus spp. | the effect of lactobacillus plantarum (fi8595), lactococcus lactis subsp. cremoris mg 1363), lactococcus lactis subsp. lactis (il 1403), and streptococcus thermophilus on cadaverine and other biogenic amine production by foodborne pathogens was investigated lysine decarboxylase broth. both of lactic acid bacteria and foodborne pathogens used (especially staphylococcus aureus, e. coli, lc. lactis subsp. lactis and lb. plantarum) had an ability to convert aminoacids into biogenic amine. the conver ... | 2012 | 22853653 |
| enterococcus faecalis enhances cell proliferation through hydrogen peroxide-mediated epidermal growth factor receptor activation. | enterococcus faecalis is a member of the intestinal and oral microbiota that may affect the etiology of colorectal and oral cancers. the mechanisms by which e. faecalis may contribute to the initiation and progression of these cancers remain uncertain. epidermal growth factor receptor (egfr) signaling is postulated to play a crucial role in oral carcinogenesis. a link between e. faecalis and egfr signaling in oral cancer has not been elucidated. the present study aimed to evaluate the associatio ... | 2012 | 22851748 |
| application of bacteriocinogenic enterococcus mundtii crl35 and enterococcus faecium st88ch in the control of listeria monocytogenes in fresh minas cheese. | several strains of enterococcus spp. are capable of producing bacteriocins with antimicrobial activity against important bacterial pathogens in dairy products. in this study, the bacteriocins produced by two enterococcus strains (enterococcus mundtii crl35 and enterococcus faecium st88ch), isolated from cheeses, were characterized and tested for their capability to control growth of listeria monocytogenes 426 in experimentally contaminated fresh minas cheese during refrigerated storage. both str ... | 2012 | 22850372 |
| structural and functional characterization of vang d-ala:d-ser ligase associated with vancomycin resistance in enterococcus faecalis. | d-alanyl:d-lactate (d-ala:d-lac) and d-alanyl:d-serine ligases are key enzymes in vancomycin resistance of gram-positive cocci. they catalyze a critical step in the synthesis of modified peptidoglycan precursors that are low binding affinity targets for vancomycin. the structure of the d-ala:d-lac ligase vana led to the understanding of the molecular basis for its specificity, but that of d-ala:d-ser ligases had not been determined. we have investigated the enzymatic kinetics of the d-ala:d-ser ... | 2012 | 22969085 |
| complete genome sequence of the porcine isolate enterococcus faecalis d32. | the complete and annotated genome sequence of enterococcus faecalis d32, a commensal strain isolated from a danish pig, suggests putative adaptation to the porcine host and absence of distinct virulence-associated traits. | 2012 | 22965105 |
| combination therapy with ampicillin and daptomycin for treatment of enterococcus faecalis endocarditis. | 2012 | 22964255 | |
| ceramide, cerebroside and triterpenoid saponin from the bark of aerial roots of ficus elastica (moraceae). | three compounds, ficusamide (1), ficusoside (2) and elasticoside (3), were isolated from the bark of aerial roots of ficus elastica (moraceae), together with nine known compounds, including four triterpenes, three steroids and two aliphatic linear alcohols. the chemical structures of the three compounds were established by extensive 1d and 2d nmr spectroscopy, mass spectrometry and by comparison with published data. the growth inhibitory effect of the crude extract and isolated compounds was eva ... | 2012 | 22963707 |
| identification and imaging of peptides and proteins on enterococcus faecalis biofilms by matrix assisted laser desorption ionization mass spectrometry. | the heptapeptide arhphph was identified from biofilms and planktonic cultures of two different strains of enterococcus faecalis, v583 and atcc 29212, using matrix assisted laser desorption ionization mass spectrometry (maldi-ms). arhphph was also imaged at the boundary of cocultured, adjacent e. faecalis and escherichia coli (atcc 25922) biofilms, appearing only on the e. faecalis side. arhphph was proteolyzed from κ-casein, a component in the growth media, by e. faecalis microbes. additionally, ... | 2012 | 22962657 |
| lack of direct effects of agrochemicals on zoonotic pathogens and fecal indicator bacteria. | agrochemicals, fecal indicator bacteria (fib), and pathogens frequently contaminate water simultaneously. no significant direct effects of fertilizer, atrazine, malathion, and chlorothalonil on the survival of escherichia coli, enterococcus faecalis, salmonella enterica, human polyomaviruses, and adenovirus were detected, supporting the assertion that previously observed effects of agrochemicals on fib were indirect. | 2012 | 22961900 |
| the lysozyme-induced peptidoglycan n-acetylglucosamine deacetylase pgda (ef1843) is required for enterococcus faecalis virulence. | lysozyme is a key component of the innate immune response in humans that provides a first line of defense against microbes. the bactericidal effect of lysozyme relies both on the cell wall lytic activity of this enzyme and on a cationic antimicrobial peptide activity that leads to membrane permeabilization. among gram-positive bacteria, the opportunistic pathogen enterococcus faecalis has been shown to be extremely resistant to lysozyme. this unusual resistance is explained partly by peptidoglyc ... | 2012 | 22961856 |
| infection of apical dentin and root-end cavity disinfection. | the purpose of this study was to assess enterococcus faecalis penetration into the dentin of the apical 3 mm and bacterial death after the application of either chlorhexidine or laser to root-end cavities. | 2012 | 22980184 |
| effectiveness of endodontic disinfecting solutions against young and old enterococcus faecalis biofilms in dentin canals. | enterococcus faecalis is a species commonly isolated from persistent root canal infections. the purpose of this study was to compare the antibacterial effects of different disinfecting solutions on young and old e. faecalis biofilms in dentin canals using a novel dentin infection model and confocal laser scanning microscopy (clsm). | 2012 | 22980181 |
| chemical composition and antimicrobial activity of essential oil of different parts of seseli rigidum. | the chemical composition and antimicrobial activity of the essential oil of the balkan endemic species seseli rigidum waldst. & kit. (apiaceae) was investigated. the monoterpene alpha-pinene was predominant in the volatile oil from aerial parts (57.4%) and fruit (23.3%). in the essential oil of the aerial parts limonene (6.7%), camphene (5.8%) and sabinene (5.5%) were also present in high amounts, and in the fruit oil, beta-phellandrene (17.4%) and sabinene (12.9%). on the contrary, the root ess ... | 2012 | 22978237 |
| coordination of thiosemicarbazones and bis(thiosemicarbazones) to bismuth(iii) as a strategy for the design of metal-based antibacterial agents. | complexes [bi(2fo4ph)cl(2)] (1), [bi(2ac4ph)cl(2)] (2), [bi(2bz4ph)cl(2)] (3), [bi(h(2)gy3dh)cl(3)] (4), [bi(h(2)gy4et)(oh)(2)cl] (5), and [bi(h(2)gy4ph)cl(3)] (6) were prepared with pyridine-2-carbaldehyde 4-phenylthiosemicarbazone (h2fo4ph), 1-(pyridin-2-yl)ethanone 4-phenylthiosemicarbazone (h2ac4ph), phenyl(pyridin-2-yl)methanone 4-phenylthiosemicarbazone (h2bz4ph), as well as with glyoxaldehyde bis(thiosemicarbazone) (h(2)gy4dh) and its 4-et (h(2)gy4et) and 4-ph (h(2)gy4ph) derivatives. the ... | 2012 | 22976983 |
| quantification of antibiotic in biofilm-inhibiting multilayers by 7.87 ev laser desorption postionization ms imaging. | the potential of laser desorption postionization mass spectrometry (ldpi-ms) imaging for small molecule quantification is demonstrated here. the n-methylpiperazine acetamide (mpa) of ampicillin was adsorbed into polyelectrolyte multilayer surface coatings composed of chitosan and alginate, both high molecular weight biopolymers. these mpa-ampicillin spiked multilayers were then shown to inhibit the growth of enterococcus faecalis biofilms that play a role in early stage infection of implanted me ... | 2012 | 23017064 |
| impact of ph on activity of trimethoprim, fosfomycin, amikacin, colistin and ertapenem in human urine. | although major impairment of activity at lower ph values has been reported for fluoroquinolones, acidification is a widely recommended practice for the prophylaxis and treatment of uncomplicated urinary tract infections (utis). until now, there is little evidence for the influence of ph on the activity on other antimicrobial classes in urine. | 2012 | 23037005 |
| bactericidal effects of nd:yag laser irradiation and sodium hypochlorite solution on enterococcus faecalis biofilm. | the aim of this study was to evaluate the bactericidal effects of nd:yag laser on biofilm of enterococcus faecalis. | 2012 | 23003122 |
| clinical analysis of cataract surgery complicated by endophthalmitis. | to assess the efficacy of vitrectomy combined with intravitreal injection in the treatment of endophthalmitis after phacoemulsification and iol implantation. | 2012 | 22993061 |
| the antibacterial efficacy of biopure mtad in root canal contaminated with enterococcus faecalis. | aim. the purpose of this in vitro study was to assess the antimicrobial efficacy of biopure mtad against e. faecalis in contaminated root canals. materials and methods. forty-two single rooted extracted human teeth were inoculated with e. faecalis and incubated for four weeks. the samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (naocl); 3% naocl; biopure mtad; 1.5% naocl/17% edta; or 3% naocl/17% edta. after a one-week incubation, ... | 2012 | 22991671 |
| rapidly progressive glomerulonephritis associated with pr3-anca positive subacute bacterial endocarditis. | patients with bacterial endocarditis often have renal complications. this report presents the case of an elderly man with rapidly progressive glomerulonephritis (rpgn) associated with subacute bacterial endocarditis (sbe) due to enterococcus faecalis infection. the patient was positive for anti-proteinase 3-antineutrophil cytoplasmic antibody (pr3-anca) and rheumatoid factor (rf) with hypocomplementemia. treatment for sbe with antibiotics and the surgical replacement of the affected valves resul ... | 2012 | 22989831 |
| [spectrum and drug sensitivity of pathogenic bacteria in children with nephrotic syndrome complicated by urinary tract infection: an analysis of 97 cases]. | to investigate the spectrum and drug sensitivity of pathogenic bacteria in children with nephrotic syndrome (ns) complicated by urinary tract infection (uti). | 2012 | 22989432 |
| inhibition of listeria monocytogenes by enterococcus mundtii isolated from soil. | two bacterial isolates with inhibitory activity against listeria monocytogenes and enterococcus faecalis were obtained from soil. genotypic and phenotypic characterization identified them as enterococcus mundtii, a species whose ability to compete with l. monocytogenes is relatively unexplored compared to other members of the genus. the thermal stability of the inhibitory factor and its sensitivity to proteolytic enzymes indicate that it is most likely a bacteriocin. both isolates grew at compar ... | 2012 | 22986201 |
| sodium chloride and potassium sorbate: a synergistic combination against enterococcus faecalis biofilms: an in vitro study. | incomplete disinfection of the root canal system is a major cause of post-treatment disease. this study aimed to investigate the disinfecting property of organic acid salts and sodium chloride (nacl), in a double-hurdle strategy, on enterococcus faecalis biofilms. first of all, the high-throughput resazurin metabolism assay (rma) was used to test a range of organic acid salts. then, to gain more insight into the efficacy of sorbate salt solutions, 48-h e. faecalis biofilms were evaluated in colo ... | 2012 | 22985004 |
| a case of daptomycin-induced immune thrombocytopenia. | we report a case of severe daptomycin-induced immune thrombocytopenia in a patient treated for methicillin-resistant staphylococcus epidermidis and ampicillin-resistant enterococcus faecalis bacteremia acquired in an intensive care unit. serum antibodies bound to platelets in the presence of daptomycin on flow cytometry. there was no evidence of other causes of thrombocytopenia. the patient died of brain herniation complicating extensive cerebral hemorrhage. to our knowledge, this is the first d ... | 2012 | 23027187 |
| water disinfection using photo-fenton: effect of temperature on enterococcus faecalis survival. | the photo-fenton process is a promising alternative to classical water disinfection treatments, although information in this regard is scarce due to its operational limitations. the effect of temperature (10, 20, 30 and 40 °c) was studied on water disinfection using the photo-fenton reaction at initial near neutral ph with resorcinol as a model of natural organic matter (nom). enterococcus faecalis, a gram-positive microorganism, was selected as an indicator of wastewater faecal contamination. t ... | 2012 | 23021523 |
| chemical composition and antimicrobial activity of the essential oil of lippia grandis schauer (verbenaceae) from the western amazon. | lippia grandis schauer is an aromatic plant that has been used as a spice in brazilian culinary and in traditional medicine to treat liver disease, disorders of the stomach and throat infections. we determined the chemical composition of the essential oil of l. grandis and evaluated its potential for the treatment of clinically-important pathogenic micro-organisms. the essential oil was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (gc-ms), giving carvacrol ( ... | 2012 | 25005969 |
| enterococcus faecalis leakage of root canal sealers: an ex vivo study. | the aim of this ex vivo study was to evaluate bacterial penetration after filling root canals using 3 different techniques. three experimental groups of 25 teeth each, obturated with lateral-warm-vertical condensation of gutta-percha, microseal technique and endorez system, respectively, were tested in a split chamber model system using enterococcus faecalis and monitored for 180 days to determine bacterial penetration. a statistical analysis was performed using the kaplan-meier method. median s ... | 2012 | 23034274 |
| synergistic effect of microbubble emulsion and sonic or ultrasonic agitation on endodontic biofilm in vitro. | irrigation dynamics and antibacterial activity determine the efficacy of root canal disinfection. sonic or ultrasonic agitation of irrigants is expected to improve irrigation dynamics. this study examined the effects of microbubble emulsion (me) combined with sonic or ultrasonic agitation on irrigation dynamics and reduction of biofilm bacteria within root canal models. | 2012 | 23063230 |
| wetting properties and critical micellar concentration of benzalkonium chloride mixed in sodium hypochlorite. | the purposes of the present study were to (1) assess the effect of the addition of benzalkonium chloride to sodium hypochlorite on its wetting properties, contact angle, and surface energy; (2) determine the critical micellar concentration of benzalkonium chloride in sodium hypochlorite; and (3) investigate the influence of addition of benzalkonium chloride on the free chlorine level, cytotoxicity, and antiseptic properties of the mixture. | 2012 | 23063229 |
| crispr-cas, a prokaryotic adaptive immune system, in endodontic, oral, and multidrug-resistant hospital-acquired enterococcus faecalis. | microorganisms are vulnerable to invasion by mobile genetic elements such as viruses, plasmids, and transposons. the recently discovered clustered regularly interspaced short palindromic repeats (crispr)-associated system, or crispr-cas, is an adaptive immunity system found in most archaea and many bacteria that targets and inactivates invading foreign genetic elements. cells with crispr-cas are more likely to resist the invasion and uptake of foreign dna such as viruses, plasmids, and transposo ... | 2012 | 23063226 |
| evaluation of microbial survival post-incidence on fresh mozzarella cheese. | commercial fresh mozzarella cheese is made by direct acidification and is stored dry or in water without salt addition. the cheese has a shelf life of 6 wk, but usually develops an off-flavor and loses textural integrity by 4 wk, potentially due to the lack of salt and high moisture that allow the outgrowth of undesirable bacteria. to understand how microbial incidence affects cheese quality and how incident pathogen-related bacteria are limited by salt level during refrigerated storage, we made ... | 2012 | 23063153 |
| a comparative study to determine the recovery rate of microorganisms of bloodstream infections: two versus three blood culture specimens. | there has been a development of automated and continuous-monitoring blood culture systems that are more sensitive than conventional systems for the detection of microorganisms. whether two or three blood culture specimens obtained during a 24-hour period using these automated systems achieving a higher recovery rate of microorganism remains to be determined. the present study was aimed to compare the recovery rates of microorganism of blood-stream infections (bsis) using two and three blood cult ... | 2012 | 23061310 |
| the par toxin-antitoxin system from enterococcus faecalis plasmid pad1 and its chromosomal homologs. | the par post-segregational killing locus present on enterococcus faecalis plasmid pad1 was the first type i toxin-antitoxin system described in gram-positive bacteria. translation of the 33 amino acid fst toxin, encoded on rna i, is suppressed by a 66 nucleotide regulatory rna, rna ii. rna i and rna ii are transcribed convergently and interact at dispersed regions of complementarity, establishing a stable complex that accumulates in plasmid-containing cells. rna ii is slowly removed from the com ... | 2012 | 23059908 |
| antibacterial activity of substituted dibenzo[a,g]quinolizin-7-ium derivatives. | berberine is a substituted dibenzo[a,g]quinolizin-7-ium derivative whose modest antibiotic activity is derived from its disruptive impact on the function of the essential bacterial cell division protein ftsz. the present study reveals that the presence of a biphenyl substituent at either the 2- or 12-position of structurally-related dibenzo[a,g]quinolizin-7-ium derivatives significantly enhances antibacterial potency versus staphylococcus aureus and enterococcus faecalis. studies with purified s ... | 2012 | 23058886 |
| propolis: a new alternative for root canal disinfection. | this study evaluated and compared colony forming units (cfus) and minimum inhibitory concentrations (mics) of calcium hydroxide and propolis as intracanal medicaments. | 2012 | 23056131 |
| removal of faecal indicator pathogens from waters and wastewaters by photoelectrocatalytic oxidation on tio(2)/ti films under simulated solar radiation. | the disinfection efficiency of water and secondary treated wastewater by means of photoelectrocatalytic oxidation (pec) using reference strains of enterococcus faecalis and escherichia coli as faecal indicators was evaluated. operating parameters such as applied potential (2-10 v), initial bacterial concentration (10(3)-10(7) cfu/ml), treatment time (up to 90 min) and aqueous matrix (pure water and treated effluent) were assessed concerning their impact on disinfection. | 2012 | 23054740 |
| a composite bacteriophage alters colonization by an intestinal commensal bacterium. | the mammalian intestine is home to a dense community of bacteria and its associated bacteriophage (phage). virtually nothing is known about how phages impact the establishment and maintenance of resident bacterial communities in the intestine. here, we examine the phages harbored by enterococcus faecalis, a commensal of the human intestine. we show that e. faecalis strain v583 produces a composite phage (φv1/7) derived from two distinct chromosomally encoded prophage elements. one prophage, prop ... | 2012 | 23045666 |
| draft genome sequence of the quality control strain enterococcus faecalis atcc 29212. | enterococcus faecalis atcc 29212, a vancomycin-sensitive strain, has been extensively used as a representative control strain for clinical and laboratory experiments. here we report the draft genome and annotation of this strain, containing 3,027,060 bp, with a g+c content of 37.2% in 126 contigs (≥ 500 bp). | 2012 | 23045510 |
| the evaluation of antimicrobial susceptibility of urine enterococci with the vitek 2 automated system in eastern turkey. | antibiotic resistant enterococci are an emerging problem, especially in urinary tract infections. the aim of the present study was to evaluate the antimicrobial susceptibility of 118 enterococci isolates from urine samples of patients admitted to malatya state hospital, a secondary care hospital in eastern turkey. the vitek 2 automated sytem was used to identify the bacteria and detect antimicrobial susceptibility to ten antibiotics: ampicillin, imipenem, ciprofloxacin, moxifloxacin, quinupristi ... | 2012 | 23077822 |
| intrinsic and acquired resistance mechanisms in enterococcus. | enterococci have the potential for resistance to virtually all clinically useful antibiotics. their emergence as important nosocomial pathogens has coincided with increased expression of antimicrobial resistance by members of the genus. the mechanisms underlying antibiotic resistance in enterococci may be intrinsic to the species or acquired through mutation of intrinsic genes or horizontal exchange of genetic material encoding resistance determinants. this paper reviews the antibiotic resistanc ... | 2012 | 23076243 |
| species-level analysis of dna sequence data from the nih human microbiome project. | outbreaks of antibiotic-resistant bacterial infections emphasize the importance of surveillance of potentially pathogenic bacteria. genomic sequencing of clinical microbiological specimens expands our capacity to study cultivable, fastidious and uncultivable members of the bacterial community. herein, we compared the primary data collected by the nih's human microbiome project (hmp) with published epidemiological surveillance data of staphylococcus aureus. | 2012 | 23071716 |
| comparative genomics of community-acquired st59 methicillin-resistant staphylococcus aureus in taiwan: novel mobile resistance structures with is1216v. | methicillin-resistant staphylococcus aureus (mrsa) with st59/sccmecv and panton-valentine leukocidin gene is a major community-acquired mrsa (ca-mrsa) lineage in taiwan and has been multidrug-resistant since its initial isolation. in this study, we studied the acquisition mechanism of multidrug resistance in an st59 ca-mrsa strain (pm1) by comparative genomics. pm1's non-β-lactam resistance was encoded by two unique genetic traits. one was a 21,832-bp composite mobile element structure (mes(pm1) ... | 2012 | 23071689 |
| synthesis and characterization of polysaccharide-maghemite composite nanoparticles and their antibacterial properties. | the aim of this study was to obtain saccharide (dextran and sucrose)-coated maghemite nanoparticles with antibacterial activity. the polysaccharide-coated maghemite nanoparticles were synthesized by an adapted coprecipitation method. x-ray diffraction (xrd) studies demonstrate that the obtained polysaccharide-coated maghemite nanoparticles can be indexed into the spinel cubic lattice with a lattice parameter of 8.35 å. the refinement of xrd spectra indicated that no other phases except the maghe ... | 2012 | 23088756 |
| antibacterial activity of substituted 5-methylbenzo[c]phenanthridinium derivatives. | antibiotic resistance has prompted efforts to discover antibiotics with novel mechanisms of action. ftsz is an essential protein for bacterial cell division, and has been viewed as an attractive target for the development of new antibiotics. sanguinarine is a benzophenanthridine alkaloid that prevents cytokinesis in bacteria by inhibiting ftsz self-assembly. in this study, a series of 5-methylbenzo[c]phenanthridinium derivatives were synthesized and evaluated for antibacterial activity against s ... | 2012 | 23084900 |
| smad4 haploinsufficiency associates with augmented colonic inflammation in select humans and mice. | smad4 is a common mediator of the tgf-beta signaling pathway. one of the members of this pathway, tgf-beta 1, has an important role in controlling gut inflammation in relation to the continuous stimulation of the intestinal microbiota. smad4 haploinsufficiency in humans has been linked to juvenile polyposis hereditary hemorrhagic telangiectasia syndrome (jp/hht; omim#17505). hematochezia and colonic mucosal inflammation suggestive of inflammatory bowel diseases (ibd) have been reported in jp/hht ... | 2012 | 23090737 |
| enterococcus and streptococcus spp. associated with chronic and self-medicated urinary tract infections in vietnam. | urinary tract infections (uti) are one of the most common infections among women worldwide. e. coli often causes more than 75% of acute uncomplicated uti, however, little is known about how recurrent utis and indiscriminate use of antimicrobials affect the aetiology of utis. this study aimed to establish the aetiology of uti in a population of recurrent and self-medicated patients referred from pharmacies to a hospital in hanoi, vietnam and to describe genotypes and antimicrobial susceptibility ... | 2012 | 23176582 |
| effectiveness of photoactivated disinfection (pad) to kill enterococcus faecalis in planktonic solution and in an infected tooth model. | the aim of this study was to investigate the effectiveness of photoactivated disinfection (pad) in killing enterococcus faecalis (ef) in planktonic solution and in an infected tooth model. | 2012 | 23095248 |
| molecular identification of an enterococcus faecalis endocarditis antigen efaa in root canals of therapy-resistant endodontic infections. | enterococcus faecalis has long been implicated in persistent root canal infections and therapy-resistant endodontic infections. it has also been associated with bacteremia, that is, infective endocarditis arising from certain invasive dental procedures. e. faecalis endocarditis antigen (efaa) has been identified as one of the principal virulence factors associated with infective endocarditis. | 2012 | 23112476 |
| comparative kinetic analysis of glycerol 3-phosphate cytidylyltransferase from enterococcus faecalis and listeria monocytogenes. | glycerol 3-phosphate cytidylyltransferase (gct) is an enzyme central to the synthesis of teichoic acids, components of the cell wall in gram positive bacteria. catalysis by gct from enterococcus faecalis and listeria monocytogenes has been investigated and catalytic properties compared. | 2012 | 23111733 |
| comparative in vitro antibacterial activity of six root canal sealers against enterococcus faecalis and proteus vulgaris. | to evaluate the antimicrobial properties of six endodontic sealers in unset and set states against enterococcus faecalis and proteus vulgaris. | 2012 | 23129142 |
| antibacterial action of calcium hydroxide vehicles and calcium hydroxide pastes. | to evaluate the in vitro action of vehicles alone and with calcium hydroxide against different bacterial species. | 2012 | 23129141 |
| 3-phenyl substituted 6,7-dimethoxyisoquinoline derivatives as ftsz-targeting antibacterial agents. | the emergence of multidrug-resistant bacteria has created an urgent need for antibiotics with a novel mechanism of action. the bacterial cell division protein ftsz is an attractive target for the development of novel antibiotics. the benzo[c]phenanthridinium sanguinarine and the dibenzo[a,g]quinolizin-7-ium berberine are two structurally similar plant alkaloids that alter ftsz function. the presence of a hydrophobic functionality at either the 1-position of 5-methylbenzo[c]phenanthridinium deriv ... | 2012 | 23127490 |
| the effect of ascorbic acid on the substantivity of tetraclean in sodium hypochlorite-treated bovine dentin. | the purpose of this study was to assess the in vitro effect of ascorbic acid on the antibacterial substantivity of tetraclean in bovine root dentin pretreated with sodium hypochlorite (naocl). | 2012 | 23119132 |
| a surveillance of high-level gentamicin-resistant enterococcal bacteremia. | enterococci have recently been recognized as a causative organism of intractable infections, including severe sepsis and infective endocarditis, in immunocompromised patients. this study investigated the epidemiological, microbiological, and prognostic characteristics of high-level gentamicin-resistant (hlgr) enterococcal bacteremia, including severe cases of infective endocarditis, in japan. a total of 155 enterococcal bacteremia episodes were identified between july 2007 and december 2009. hlg ... | 2012 | 23115941 |
| the influence of chlorhexidine on the antibacterial effects of mta. | to investigate the influence of chlorhexidine in different concentrations when mixed with mineral trioxide aggregate gray (mta-g) or white (mta-w). the antibacterial effect on enterococcus faecalis was used as the primary parameter. | 2012 | 23115769 |
| evaluation of a pcr assay to detect enterococcusfaecalis in blood and determine glycopeptides resistance genes: van a and van b. | bacteremia due to enterococcus faecalis is usually caused by strains resistant to most antibiotics. effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. the aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococcal bacteremia and its sensitivity pattern to antimicrobial drugs. | 2012 | 23115452 |
| crystallization and first data collection of the putative transfer protein tran from the gram-positive conjugative plasmid pip501. | conjugative plasmid transfer is the most important route for the spread of resistance and virulence genes among bacteria. consequently, bacteria carrying conjugative plasmids are a substantial threat to human health, especially hospitalized patients. whilst detailed information about the process has been obtained for gram-negative type-4 secretion systems, little is known about the corresponding mechanisms in gram-positive (g+) bacteria. the successful purification and crystallization of the put ... | 2012 | 23143259 |
| zaaps program results for 2010: an activity and spectrum analysis of linezolid using clinical isolates from 75 medical centres in 24 countries. | the zyvox® annual appraisal of potency and spectrum (zaaps) program monitors the in vitro activities of linezolid and comparator agents for gram-positive organisms in latin america, europe, canada, and the asia-pacific. for the 2010 program a total of 6305 gram-positive strains were collected from 75 medical centres on five continents (24 countries). reference broth microdilution susceptibility tests were performed on organisms from the following groups: staphylococcus aureus (2875), coagulase n ... | 2012 | 23174097 |
| resistance profiles of urinary tract infections in general practice--an observational study. | guideline recommendations on therapy in urinary tract infections are based on antibiotic resistance rates. due to a lack of surveillance data, little is known about resistance rates in uncomplicated urinary tract infection (uti) in general practice in germany. in a prospective observational study, urine cultures of all women presenting with urinary tract infections in general practice were analysed. resistance rates against antibiotics recommended in german guidelines on uti are presented. | 2012 | 23171154 |
| evaluation of the bactericidal effect of nd:yag, er:yag, er,cr:ysgg laser radiation, and antimicrobial photodynamic therapy (apdt) in experimentally infected root canals. | in recent years, various laser systems have been introduced into the field of laser-assisted endodontic therapy. the aim of this study was to evaluate the bactericidal effect of nd:yag, er:yag, er,cr:ysgg laser radiation, and antimicrobial photodynamic therapy (apdt) in experimentally infected root canals compared with standard endodontic treatment of 5.25% sodium hypochlorite (naclo) irrigation. | 2012 | 23168798 |