Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| identification of vibrio vulnificus o serovars with antilipopolysaccharide monoclonal antibody. | a serotyping scheme for vibrio vulnificus predicated on the detection of lipopolysaccharide (lps) antigens is proposed. the serovar o typing scheme used to type v. vulnificus employs polyclonal antisera raised in rabbits immunized with heat-killed whole-cell vaccines. polyclonal typing sera produced in this manner cross-react with heterologous strains. affinity purification of polyclonal antisera with lps affinity columns resolved some of these cross-reactions; however, affinity-purified polyclo ... | 1991 | 1761690 |
| formation of nonculturable vibrio vulnificus cells and its relationship to the starvation state. | entry into the viable but nonculturable state by the human bacterial pathogen vibrio vulnificus in artificial seawater microcosms was studied. in contrast to the long-term culturability exhibited by cells incubated under these starvation conditions at room temperature, cells exposed to a temperature downshift to 5 degrees c exhibited an immediate decrease in culturability. cells incubated at low temperature exhibited a morphological change from rods to cocci but demonstrated no reductive divisio ... | 1991 | 1768138 |
| use of the polymerase chain reaction in detection of culturable and nonculturable vibrio vulnificus cells. | vibrio vulnificus is a human pathogen associated with consumption of raw oysters. during the colder months the organism apparently enters a viable but nonculturable state and thus cannot be cultured by ordinary bacteriological methods. for this reason, another means of detecting this bacterium is necessary. in the present study we utilized the polymerase chain reaction (pcr) to detect v. vulnificus dna, thus eliminating the problem of nonculturability. dna from both culturable and nonculturable ... | 1991 | 1768140 |
| analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development. | using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ... | 1991 | 1769342 |
| vibrio vulnificus. hazard on the half shell. | vibrio vulnificus is an extremely invasive gram-negative bacillus that causes bacteremia and shock. it should be suspected in any patient who is immunocompromised or has liver disease or hemochromatosis. reduced gastric acidity may also increase the risk of infection if a patient presents with a history of ingesting raw shellfish (especially oysters) or trauma in brackish waters and skin lesions. patients most commonly present with one of three clinical syndromes: primary septicemia, wound infec ... | 1991 | 1771878 |
| a lethal leviathan--vibrio vulnificus. | 1991 | 1771890 | |
| vibrio vulnificus peritonitis. a unique case. | louisiana is justifiably famous for the seafood harvested from its coastal waters. unfortunately, undesirables accompany this harvest. the author presents a case of peritonitis due to vibrio vulnificus which followed ingestion of raw oysters. | 1991 | 1779190 |
| physiological effects of the lipopolysaccharide of vibrio vulnificus on mice and rats. | vibrio vulnificus is a pathogenic, marine, gram-negative bacterium which commonly enters and infects humans via open wounds or the ingestion of raw seafood. the lipopolysaccharide (lps) of v. vulnificus has recently been characterized, but the biological activity of this putative endotoxin is unknown. three treatment groups were used to test its activity: saline (negative control), the lps of salmonella typhimurium (a known endotoxin), and the lps of v. vulnificus. whereas, intravenous injection ... | 1991 | 1779875 |
| [biological characteristics of cultured papilla cells--localization of androgen receptors and chemotactic factor(s) for epithelial cells]. | we have reported that cultured papilla cells (pcs) grown by isolation and cultivation of human hair papillae show some biological characteristics. in the present report, some important biological characteristics of pcs are showed. 1) localization of androgen receptors on pcs localization of androgen binding protein in pcs was examined. cytochemical staining of pcs using dht-peroxidase conjugate gave positive reactions in the niclei of pcs originating from scalp and axilla-dermal papillae. these ... | 1991 | 1782180 |
| extensive cellulitis with septic shock caused by vibrio vulnificus infection--a case report with review of literature. | 1991 | 1791352 | |
| unconjugated and conjugated bilirubin pigments during perinatal development. iii. studies on serum of breast-fed and formula-fed neonates. | the process of conjugation and secretion of bilirubin was studied in a group of healthy, full-term, exclusively breast-fed newborns and a control group of exclusively formula-fed infants by means of a reverse-phase high-performance liquid chromatographic analysis of bilirubins present in serum. the serum concentrations of unconjugated bilirubin, esterified bilirubin, and the proportion of diesterified bilirubin (as percent of esterified bilirubin) were not significantly different in breast- and ... | 1991 | 1797114 |
| unconjugated and conjugated bilirubin pigments during perinatal development. iii. studies on serum of breast-fed and formula-fed neonates. | the process of conjugation and secretion of bilirubin was studied in a group of healthy, full-term, exclusively breast-fed newborns and a control group of exclusively formula-fed infants by means of a reverse-phase high-performance liquid chromatographic analysis of bilirubins present in serum. the serum concentrations of unconjugated bilirubin, esterified bilirubin, and the proportion of diesterified bilirubin (as percent of esterified bilirubin) were not significantly different in breast- and ... | 1991 | 1797114 |
| a primary demyelinating disorder of young cattle. | the clinical signs and neuropathological changes are described in six limousin x calves that, at about one month after birth, showed signs of blindness, nystagmus, rotation of the eyes, opisthotonos, hyperprotraction of the forelegs and, in one case, apparent seizures. pathologically there was necrosis of the optic chiasma and focal areas of myelin sheath vacuolation or demyelination in certain areas of the brain, especially in the cerebellar peduncles. in one animal, kept alive for 7 months, th ... | 1991 | 1800911 |
| structure of the rabbit phospholamban gene, cloning of the human cdna, and assignment of the gene to human chromosome 6. | we have isolated and characterized genomic dna clones encoding rabbit phospholamban. only a single gene for phospholamban was detected in the rabbit genome. the phospholamban gene of 13.2 kilobases contains only one 10.5-kilobase intron, which separates exonic sequences located in the 5'-untranslated region. two potential transcription initiation sites were mapped to 335 and 185 nucleotides upstream from the translation initiation site in the mrna or 239 and 89 nucleotides upstream from the exon ... | 1991 | 1828805 |
| complexity and sequence identification of 24 rat v beta genes. | twenty-four tcr v beta genes were cloned by anchored pcr from the lewis rat strain and identified by nucleotide and amino acid sequence comparisons to known mouse v beta genes. rat v beta genes exist in 17 single-member and 3 multimember subfamilies and exhibit 86 to 94 and 72 to 92% nucleotide and amino acid sequence similarities, respectively, to their mouse counterparts. a single rat gene, designated v beta 20, having no previously known mouse counterpart was identified; a closely related gen ... | 1991 | 1828824 |
| purified protein derivative of mycobacterium tuberculosis and excretory-secretory antigen(s) of toxocara canis expand in vitro human t cells with stable and opposite (type 1 t helper or type 2 t helper) profile of cytokine production. | a large series of t cell clones (tcc) specific for purified protein derivative (ppd) of mycobacterium tuberculosis (total 60) or toxocara canis excretory/secretory (tes) antigen (total 69) were established from the peripheral blood of two healthy individuals and analyzed for their profile of cytokine production in response to stimulation with either the specific antigen or the polyclonal activator phorbol myristate acetate plus anti-cd3 antibody. under both these experimental conditions, the gre ... | 1991 | 1829097 |
| the extracellular cytolysin of vibrio vulnificus: inactivation and relationship to virulence in mice. | the ca. 51-kda extracellular cytolysin of vibrio vulnificus has been proposed as a virulence factor. we inactivated the structural gene for cytolysin in fully virulent, clinical v. vulnificus strains by both transposon mutagenesis and marker exchange techniques. inactivation of the cytolysin did not affect virulence in our mouse models. the 50% lethal dose of cytolysin-negative strains was comparable to that of the cytolysin-positive parent strains after intraperitoneal inoculation with and with ... | 1991 | 1846124 |
| growth arrest induced by transforming growth factor beta 1 is accompanied by protein phosphatase activation in human keratinocytes. | protein phosphorylation and dephosphorylation are involved in regulation of cell growth. we tested the hypothesis that the growth inhibitory effect of transforming growth factor beta 1 (tgf-beta 1) involves activation of protein phosphatases. exposure of human keratinocytes in culture to 400 pm tgf-beta 1 for 48 h led to 80% inhibition of dna synthesis as measured by nuclear labeling. incubation of cultured keratinocytes with 400 pm tgf-beta 1 rapidly activated (within 30 min) protein serine/thr ... | 1991 | 1847373 |
| absence of na+,k(+)-atpase regulation of endosomal acidification in k562 erythroleukemia cells. analysis via inhibition of transferrin recycling by low temperatures. | transferrin (tf) acidification has been shown to be limited to ph 6 in murine balb/c 3t3 fibroblasts, human a549 epidermoid carcinoma cells, and chinese hamster ovary cells and is followed by alkalinization during recycling. in contrast, tf acidification in the human erythroleukemic cell line k562 proceeds to below ph 5.5, and alkalinization of internal tf during recycling is not observed. to explore the regulation of endosomal ph in k562 cells, we determined whether the existence of an early en ... | 1991 | 1847374 |
| cyclic amp-elevating agents block chemoattractant activation of diradylglycerol generation by inhibiting phospholipase d activation. | agents which elevate cellular camp (prostaglandin e2, theophylline, and forskolin) or mimic camp action (dibutyryl camp) are known to inhibit human neutrophil activation (superoxide generation and secretion) by receptor-linked agonists such as formyl-methionyl-leucyl-phenylalanine (fmlp). herein, we show that these agents also markedly inhibit fmlp-stimulated diradylglycerol generation (assayed by mass methods). the magnitude of inhibition correlated with the ability of a given agent or combinat ... | 1991 | 1847376 |
| development of circadian sleep-wake rhythms in preterm and full-term infants. | we have compared the roles of neurologic maturity and environmental time cues in the development of the entrained circadian sleep-wake rhythm in the preterm and term human infant. the preterm infants (n = 19) spent some time after birth in a hospital nursery with no environmental time cues, whereas the term infants (n = 22) were exposed from birth to a cyclical light and dark environment with one major caregiver. the circadian sleep-wake rhythm in the preterm infants entrained after a similar ti ... | 1991 | 1852533 |
| signal transduction mechanism of interleukin 6 in cultured rat mesangial cells. | interleukin 6 (il-6) is one of the potent autocrine growth factors for mesangial cells. we investigated the signal transduction mechanism of il-6 in cultured rat mesangial cells. il-6 induced a transient increase of inositol 1,4,5-trisphosphate (ins 1,4,5-p3) followed by a transient and sustained increase of intracellular calcium concentration, suggesting that il-6 stimulates phosphoinositide turnover. il-6 also stimulated prostaglandin e2 (pge2) production. the il-6-concentration dependency in ... | 1991 | 1855592 |
| [microbial spectrum and microbiological diagnosis of infectious endocarditis]. | still streptococci play the most important role as causatives of infective endocarditis. as change in composition of patient groups has taken place gradually pathogens like staphylococcus epidermidis, enterococci, gram-negative rods and candida species become more and more important now. providing high-level accuracy if applied according to current rules blood culture is yet the basis for endocarditis caused by popular bacterial pathogens. if infection due to candida species is suspected additio ... | 1991 | 1855807 |
| in vitro effect of phyllanthus amarus on hepatitis b virus. | to evaluate the effects of p. amarus on hepatitis b virus (hbv) antigens and hbv-dna, initial ethanolic extract and subsequent fractions of the plants were prepared. the whole plant material was dried, powdered and extracted with alcohol and subsequently fractionated in hexane, chloroform, butanol and finally in water. all the material were tested for in vitro effects on hbsag, hbeag and hbv-dna in serum samples positive for hbv antigens followed by the screening of respective antigens by elisa. ... | 1991 | 1855821 |
| resuscitation of vibrio vulnificus from the viable but nonculturable state. | stationary-phase-grown cells of the estuarine bacterium vibrio vulnificus became nonculturable in nutrient-limited artificial seawater microcosms after 27 days at 5 degrees c. when the nonculturable cells were subjected to temperature upshift by being placed at room temperature, the original bacterial numbers were detectable by plate counts after 3 days, with a corresponding increase in the direct viable counts from 3% to over 80% of the total cell count. no increase in the total cell count was ... | 1991 | 1860818 |
| vibrio vulnificus sepsis presenting as leg pain and lower extremity rash. | 1991 | 1863312 | |
| hemochromatosis, iron and septicemia caused by vibrio vulnificus. | vibrio vulnificus is killed by normal human blood but grows rapidly in blood from patients with hemochromatosis. it also grows in normal blood if the saturation of the transferrin is increased or if hematin, which contains iron, is added. it is suggested that the increased availability of iron in the blood of patients with chronic iron overload is responsible for their enhanced susceptibility to infection with v vulnificus. | 1991 | 1872665 |
| functional expression of furin demonstrating its intracellular localization and endoprotease activity for processing of proalbumin and complement pro-c3. | we have cloned a rat cdna encoding furin which is structurally related to yeast kex2 protease. products of 88 and 94 kda were obtained by in vitro transcription/translation of the cdna in the absence and presence of microsomes. when the cdna was transfected into cos-1 cells, furin was expressed as a major glycosylated form of 94 kda, accompanied by a minor proteolytic form of 86 kda, and found to be localized in the golgi complex. proalbumin and complement pro-c3 are intracellularly processed in ... | 1991 | 1885622 |
| calcium regulation of the human pmn cytosolic 15-lipoxygenase. | addition of tracer (pg) amounts of [3h]arachidonic acid to the 120,000 x g cytosolic fraction of human polymorphonuclear leukocytes (pmns) produced [3h]-15-hete, the product of the 15-lipoxygenase, as the major metabolite. in the presence of nanomolar and low micromolar amounts of calcium, [3h]-15-hete formation was increased as much as 15-fold which corresponded to 17% conversion of added substrate. this enhancement of the cytosolic 15-lipoxygenase activity, which was reversible by egta, was in ... | 1991 | 1892881 |
| [vibrio vulnificus]. | a patient sustained a knife wound while cleaning eels and subsequently severe wound infection developed. vibrio vulnificus was isolated from the wound and, on the basis of this, the main features of the epidemiology of the bacteria and the typical clinical pictures are reviewed. | 1991 | 1897050 |
| purification and properties of a neurotensin-degrading endopeptidase from pig brain. | neurotensin (nt) endopeptidase (ec 3.4.24.16) has been purified about 800-fold from pig brain by four sequential chromatographic steps depending on ion-exchange and hydrophobic interactions. two types of preparation were studied: one from a triton x-100-solubilized membrane fraction, and the other from the soluble fraction containing 90% or more of the total activity in the homogenate. nt endopeptidase activity was monitored by high-precision liquid chromatography of the two peptide products, ch ... | 1991 | 1905921 |
| control of enterococcus faecalis sex pheromone cad1 elaboration: effects of culture aeration and pad1 plasmid-encoded determinants. | aeration of plasmid-free enterococcus faecalis strains resulted in an 8- to 16-fold decrease in sex pheromone cad1 activity in culture filtrates. levels of two unrelated pheromones, cpd1 and cam373, were unaffected by culture aeration. aeration also resulted in a decrease in the expression of conjugative transfer functions observed in cells containing pad1 trab mutations, verifying a link between trab function and pheromone "shutdown." tests with a series of pad1 mini-plasmids indicated that the ... | 1991 | 1924555 |
| drug use during breast-feeding. | the physicochemical and pharmacokinetic factors involved in transfer of drugs into breast milk are reviewed. passage of drugs into milk can best be viewed as a two-compartment system. various ratios of the drug concentration values in the two compartments and ratios of maternal-to-infant intake have been described. knowledge of the limitations of these methods is necessary to properly interpret and apply the literature on drug excretion into breast milk. factors involved in choosing a drug for a ... | 1991 | 1934918 |
| growth of vibrio vulnificus in serum from alcoholics: association with high transferrin iron saturation. | 1991 | 1940460 | |
| a case of vibrio vulnificus septicaemia acquired in victoria. | the clinical features and laboratory findings of a case of vibrio vulnificus septicaemia are reported. the illness occurred in a previously well 68-year-old man who was accidentally spiked in the buttock by the dorsal spine of a flathead caught in tamboon inlet, near mallacoota, victoria. the clinical picture of an acute septicaemic illness with shock, associated with metastatic cellulitic lesions on the lower limbs progressing to bulla formation, skin necrosis, necrotising fasciitis and myositi ... | 1991 | 1988798 |
| pulmonary infiltrates associated with vibrio vulnificus septicemia. | in the case described, fatal vibrio vulnificus primary septicemia was complicated by development of diffuse pulmonary infiltrates. clinical features of this recently discovered infectious disease are discussed. various aspects of diagnoses of pulmonary infiltrates in patients with v vulnificus septicemia are also presented. vibriosis should be suspected in patients with chronic, underlying, predisposing diseases, recent ingestion of raw seafood, and characteristic skin lesions. | 1991 | 1997461 |
| purification and characterization of a secreted protease from the pathogenic marine bacterium vibrio anguillarum. | vibrio anguillarum is a pathogenic marine bacterium which causes the disease vibriosis in salmonid fish, which is characterized by a fatal hemorrhagic septicemia accompanied by massive tissue destruction. in this paper, the purification of the major caseinolytic extracellular protease from v. anguillarum is presented. the purification steps include ammonium sulfate precipitation, deae-sepharose chromatography, sephacryl s-200 chromatography, and deae high-pressure liquid chromatography. the puri ... | 1991 | 2012804 |
| ozone depuration of vibrio vulnificus from the southern quahog clam, mercenaria campechiensis. | southern quahog clams, mercenaria campechiensis, were dosed with vibrio vulnificus and placed in a pilot-scale depuration system using ozonated recirculated artificial seawater. twenty-four hours of treatment with ozone-treated recirculating artificial seawater reduced the numbers of v. vulnificus in the shellfish meats by an average of 2 log units when compared to natural die-off in control clams. the oxidant levels (up to 3 mg/liter) did not adversely affect shellfish pumping during the depura ... | 1991 | 2022868 |
| polymerase chain reaction identification of vibrio vulnificus in artificially contaminated oysters. | dnas extracted from vibrio vulnificus seeded into oyster homogenates were evaluated as templates for the polymerase chain reaction. several extraction procedures were examined, and it was determined that dna recovered from cells lysed by guanidine isothiocyanate, extracted with chloroform, and precipitated with ethanol was most suitable for use as a polymerase chain reaction template. the region targeted was a 519-bp portion of the cytotoxin-hemolysin gene of v. vulnificus. this region was ampli ... | 1991 | 2039231 |
| the economics of spaying and neutering: market forces and owners' values affecting pet population control. | 1991 | 2045338 | |
| enzyme immunoassay for identification of vibrio vulnificus in seawater, sediment, and oysters. | historically, methods used to identify vibrio vulnificus in environmental samples have been inadequate because isolation and identification procedures are time-consuming and fail to separate v. vulnificus from other bacterial species. we describe an enzyme immunoassay (eia) and culture techniques which identified v. vulnificus in seawater, sediment, and oysters. the eia used monoclonal antibody frbt37 to a species-specific epitope of v. vulnificus. no cross-reactions were observed among 72 non-v ... | 1991 | 2059045 |
| vibrio vulnificus septicemia in korea: clinical and epidemiologic findings in seventy patients. | we studied the clinical characteristics and the epidemiology of primary septicemia associated with vibrio vulnificus in 70 patients. all patients came from the western and southern coastal areas of korea. most cases (96%) occurred during the summer months, in men (96%), and in persons 40 or more years of age (90%). the illness of 46 patients (66%) began with septicemia, often within 2 days of the consumption of raw seafood. forty-seven patients (67%) had preexisting hepatic disease, and 49 (70%) ... | 1991 | 2061435 |
| electrophoretic analysis of lipopolysaccharide isolated from opaque and translucent colony variants of vibrio vulnificus using various extraction methods. | lipopolysaccharides (lps) from an opaque and a translucent colony variant of vibrio vulnificus were isolated by several methods and the electrophoretic profiles were analysed. the phenol-water extraction method provided a better yield compared to the phenol-chloroform-petroleum ether method. in addition, two rapid micro-assays were used to isolate lps for electrophoretic analysis. the electrophoretic pattern was the same for all lps extracts and was similar in both variants. no high molecular we ... | 1991 | 2062249 |
| mediastinal tumors. iii. cystic thymoma. | 1991 | 1003021 | |
| hyperinsulinism due to pancreatic islet cell hyperplasia: report of a case in an adult. | 1991 | 183635 | |
| informational-motivational components of a model's demonstration. | 1991 | 270184 | |
| [clinical observation and treatment of 115 cases of mixed salivary gland tumors]. | 1991 | 208195 | |
| assertiveness workbook: when it's best not to assert. | 1991 | 257725 | |
| the life-after-death phenomenon. | the life-after-death phenomenon has created student interest and is often a topic initiated by students by students in death education courses. the article summarizes some popular publications about the life-after-death phenomenon and extracts commonalities that potentially affect other topic areas in a death education course or unit. topic areas such as coping with death, suicide and stages of dying are then examined to note the effect the phenomenon can have on the topical discussions. the art ... | 1990 | 258721 |
| oysters, iron overload and vibrio vulnificus septicaemia. | a case of vibrio vulnificus septicaemia complicated by cutaneous leg ulceration is described. a 74 year old man with haemochromatosis and sideroblastic anaemia developed an acute febrile illness with cutaneous manifestations 24 hours after ingesting raw oysters. the presence of blistering should be considered an important clue to the diagnosis of vibrio vulnificus septicaemia, and this can facilitate prompt effective antimicrobial therapy. clinicians should be aware of this infection because of ... | 1990 | 2073206 |
| enhancement of virulence of two environmental strains of vibrio vulnificus after passage through mice. | the virulence of two environmental strains of vibrio vulnificus to iron-loaded adult mice was enhanced by passage through mice. estimates of 50% lethal dose values (ld50) determined by end point titration were reduced 100- and 1000-fold for the two strains. passage through mice also selected for the opaque colony type phase variation of v. vulnificus, reported by others to be more virulent to mice than a translucent colony type. | 1990 | 2076589 |
| permeability-enhancing action of vibrio vulnificus protease and its control system. | 1990 | 2101153 | |
| phenotypic evaluation of acapsular transposon mutants of vibrio vulnificus. | translucent, avirulent spontaneous phase variants of vibrio vulnificus mo6-24 reverted back to the original opaque, encapsulated phenotype under both in vivo and in vitro conditions. two translucent, acapsular mutants, which did not show phase variation, were constructed by using the transposon tn5 is50l::phoa (tnphoa). loss of capsule was accompanied by decreases in virulence, hydrophilicity, and serum resistance. the ability to utilize transferrin-bound iron for growth was lost in only one of ... | 1990 | 2160432 |
| kinetics of the reaction of prostaglandin h synthase compound ii with ascorbic acid. | the reduction of prostaglandin h synthase compound ii by ascorbic acid in the presence of diethyldithiocarbamate was studied in 0.1 m phosphate buffer (ph 8.0) at 4.0 +/- 0.5 degrees c, by rapid scan spectrometry and transient state kinetics. a saturation effect and nonzero intercept were observed in the plot of pseudo-first-order rate constant versus ascorbic acid concentration. the saturation behavior suggests formation of a complex between prostaglandin h synthase compound ii and ascorbic aci ... | 1990 | 2168160 |
| characterization of two low em forms of cytochrome a3 and their carbon monoxide complexes in mammalian cytochrome c oxidase. | evidence is presented for the existence of two forms of low-potential cytochrome a3. one appears to be similar to the low-spin form reported by nicholls, p., and v. hildebrandt (1978 biochem. j. 173:65-72) and wrigglesworth, j. m., j. elsden, a. chapman, n. van der water, and m. f. grahn (1988. biochim. biophys. acta. 936:452-464). it has a reduced soret peak near 428 nm and a prominent alpha peak near 602 nm. this form is seen when the enzyme is either supplemented with lipoprotein or incorpora ... | 1990 | 2168220 |
| role of oxygen free radical formation in the mechanism of menogaril resistance in multidrug resistant tumor cells. | the mechanisms of action and resistance to menogaril, a clinically active anthracycline antitumor drug, were evaluated in sensitive and doxorubicin-selected multidrug resistant human breast tumor (mcf-7) cell lines. while mcf-7/adrr cells were highly resistant (250-500-fold) to doxorubicin, they displayed only marginal resistance (10-fold) to menogaril. in contrast to doxorubicin, the mechanism of resistance to menogaril in these cells does not involve differential inhibition of dna synthesis as ... | 1990 | 2168296 |
| mechanisms underlying the stimulation of pth release by gppnhp in permeabilized bovine parathyroid cells. | we examined changes in camp and inositol phosphate metabolism to assess the contribution of the guanine nucleotide regulatory (g) protein(s) regulating adenylate cyclase and phospholipase c in mediating the stimulatory effects of gppnhp on pth release from permeabilized bovine parathyroid cells. to examine the role of gs, the g protein stimulating adenylate cyclase, and camp on pth release, permeabilized cells were incubated with either gppnhp or isoproterenol, and the effects of these agents on ... | 1990 | 2168660 |
| proton-nuclear magnetic resonance relaxation times in brain edema. | proton relaxation times of protein solutions, bovine brain, and edematous feline brain tissue were studied as a function of water concentration, protein concentration, and temperature. in accordance with the fast proton exchange model for relaxation, a linear relation could be established between r1 and the inverse of the weight fraction of tissue water. this relation also applied to r2 of gray matter and of protein solutions. no straightforward relation with water content was found for r2 of wh ... | 1990 | 2168666 |
| [a case of fatal sepsis due to vibrio vulnificus]. | a 66-year-old male with chronic alcoholic liver injury was admitted on july 27, 1986 to our hospital with complaints of high fever, convulsion and skin erythema. he had consumed raw fish 3 days before, and had a scratch wound over the right arm and left leg because he had slipped in a small stream in the woods the day before admission. he was already in shock state with sepsis of v. vulnificus and dic on admission. although the treatment with abpc, cp, caz, mino for sepsis, and heparin & antithr ... | 1990 | 2187037 |
| in vitro activity of ljc10,627, a new carbapenem antibiotic with high stability to dehydropeptidase i. | the in vitro activity of ljc10,627, a new carbapenem, was compared with those of imipenem and ceftazidime. ljc10,627 had broad-spectrum activity against gram-positive and gram-negative clinical isolates. the mics of this compound for 90% of members of the family enterobacteriaceae tested (mic90s), including strains resistant to ceftazidime, ranged from 0.1 to 25 micrograms/ml. ljc10,627 inhibited pseudomonas aeruginosa at an mic90 of 3.13 micrograms/ml; it thus was twofold more active than imipe ... | 1990 | 2203313 |
| massive rhabdomyolysis: a rare presentation of primary vibrio vulnificus septicemia. | 1990 | 2220890 | |
| abelson murine leukemia virus transforms preneoplastic emu-myc transgene-carrying cells of the b-lymphocyte lineage into plasmablastic tumors. | e mu-myc transgenic mice were back-crossed to balb/c mice up to back-cross generation 3. the offspring that included transgene-carrying and -negative mice in approximately equal proportions were randomly divided into 2 groups. thirty-four mice (group i) were treated with pristane, followed by a-mulv, and 40 (group ii) were injected with a-mulv alone. altogether, 16 lymphoid tumors developed in group i and 17 in group ii. nine of the tumors in group i and 4 in group ii appeared as ascitic tumors. ... | 1990 | 2228313 |
| neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats. | intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ... | 1990 | 2245800 |
| neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats. | intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ... | 1990 | 2245800 |
| proteolytic processing of porcine big endothelin-1 catalyzed by cathepsin d. | cathepsin d, a candidate for endothelin-converting enzyme (ece), was allowed to act on porcine big endothelin-1 (big et-1, 1-39). the proteinase primarily cleaved the asp18-ile19 and trp21-val22 bonds of big et-1(1-39), with a optimum ph of 3.5. the mature et-1(1-21), generated by the cleavage between trp21 and val22, was subsequently degraded by removal of most of the c-terminal tripeptide (ile19-ile20-trp21). therefore, cathepsin d is by no means a specific ece, although the proteinase does cl ... | 1990 | 2268325 |
| a double-blind comparison of 0.5% bupivacaine and 0.75% ropivacaine administered epidurally in humans. | in an attempt to compare equipotent doses of epidurally administered bupivacaine and ropivacaine, 44 healthy patients, aged 18-70 years, undergoing lower extremity orthopedic procedures were studied in a randomized, double-blind manner. twenty-one patients received a single epidural injection of 20 ml 0.5% bupivacaine at the l23 or l34 interspace and 23 patients received 20 ml 0.75% ropivacaine in a similar manner. onset of and recovery from sensory anesthesia and motor block were recorded. no s ... | 1990 | 2271468 |
| a successfully treated case of vibrio vulnificus septicemia with shock. | vibrio vulnificus infection often causes serious or fatal disease. recently, in japan there have been numerous reports of vibrio vulnificus infection. here, we report a successfully treated case of vibrio vulnificus septicemia with shock, disseminated intravascular coagulation (dic) and necrotizing cellulitis in a middle-aged heavy drinker with chronic alcoholic liver disease. on reviewing 38 cases in japan including ours, the overall mortality rate was 68%. although the incidence is relatively ... | 1990 | 2273613 |
| incidence of vibrio vulnificus in northern new england water and shellfish. | vibrio vulnificus, an autochthonous inhabitant of the estuarine environment, was detected in water and oysters from the great bay estuary system of new hampshire and maine. previously, it had not been detected north of boston harbor on the east coast of the united states. v. vulnificus was detected in water and shellfish samples at five out of ten sites, and only in areas that were not open to recreational shellfishing. although samples were collected from may into december, v. vulnificus was on ... | 1990 | 2283033 |
| [bronchial hyperresponsiveness to histamine in guinea pig induced by intravenous administration of platelet activating factor]. | platelet activating factor (paf) has recently been demonstrated to play an important role in allergic diseases such as bronchial asthma. changes in airway wall thickness have recently been suggested to cause excess airway narrowing of asthma. in this study, the authors examined the bronchial hyperresponsiveness to histamine in guinea pig induced by intravenous administration of paf by measuring dynamic compliance and dynamic respiratory resistance. moreover, a new formula which can exclude the e ... | 1990 | 2290229 |
| generation of affinity for antithrombin iii by supplemental sulfation of heparin species with low affinity for the protein. | the tributylammonium salt of a porcine heparin subfraction with low affinity for antithrombin iii (mr 7,500-18,000; anti-clotting activity, 7 usp units/mg), having degrees of sulfate substitution at d-glucosamine and l-iduronic acid residues of glcns 0.786, glcn6s 0.628, and idoa2s 0.682 mol, was reacted with 10 or 20 mol of pyridine-sulfur trioxide per mol equiv. of available hydroxyl groups in n,n-dimethylformamide at -10 degrees c for 1 h. both chemical and nmr spectroscopic analyses revealed ... | 1990 | 2292586 |
| vibrio vulnificus wound infection treated successfully with oral ciprofloxacin. | 1990 | 2299188 | |
| osteomyelitis caused by vibrio vulnificus. | 1990 | 2299219 | |
| polyvibrio infections: vibrio vulnificus and vibrio parahaemolyticus dual wound and multiple site infections. | 1990 | 2299220 | |
| vibrio vulnificus infections in the hand. report of three patients. | 1990 | 2303515 | |
| digestibility of cotton plant by-product diets for sheep at two levels of intake. | crossbred wethers (n = 48) were assigned randomly to 1 of 12 experimental diets, arranged in a 3 x 2 x 2 split plot to evaluate three levels of cotton plant by-product (25, 50 and 75% cpb), two levels of naoh treatment of cpb (0 and 4%) and two levels of monensin supplementation (0 and 30 ppm, dm basis) at two intake levels (70 and 90% of ad libitum). intake was a within-lamb variable. ad libitum intake was established over an initial 17-d adjustment period. feed intake and fecal output were rec ... | 1990 | 2312420 |
| primary vibrio vulnificus sepsis in kentucky. | vibrio vulnificus is associated with infection acquired during contact with sea water or with seafood, and is seldom suspected by physicians in noncoastal states. the ease of transportation of fresh raw seafood has facilitated this organism's capacity to produce disease in geographic areas in which it was previously unseen. we have reported a case of fatal vibrio vulnificus sepsis acquired from ingestion of fresh oysters in the inland united states. | 1990 | 2315791 |
| long-term clearance of inhaled uo2 particles from the pulmonary region of the rat. | this paper reports on an enriched uranium dioxide (uo2) mass clearance study undertaken with fischer-344 rats. the uo2 had a uranium (u) isotopic composition of 0.79% 234u, 92.8% 235u, 0.34% 236u and 6.06% 238u, by mass, with an alpha-particle activity of 1.91 bq micrograms-1. forty-six rats were exposed to an enriched uo2 aerosol that had an activity median particle aerodynamic diameter ranging from 2.7 to 3.2 microns. the rats were killed from 1 to 720 d post-inhalation (pi). the mass of enric ... | 1990 | 2323929 |
| casein and casein subunits in preterm milk, colostrum, and mature human milk. | human milk proteins have both nutritional and physiological roles for the breast-fed infant. while the biochemistry and developmental patterns for many whey proteins are well known, our knowledge of human casein and its subunits is still limited. we have recently developed a method to isolate casein from whey proteins in human milk and to separate the casein subunits by fast protein liquid chromatography. in this study we have applied this methodology to study the casein subunit pattern in prete ... | 1990 | 2358977 |
| blockade of inhibition in a pathway with dual excitatory and inhibitory action unmasks a capability for ltp that is otherwise not expressed. | long-term potentiation (ltp) can be readily elicited in a number of hippocampal pathways, but has not been seen in the dentate commissural pathway. the dentate commissural pathway is similar to the commissural/schaffer collateral projection to ca1 except that it produces powerful inhibition that occurs nearly concurrently with the excitation. the present study evaluates whether this inhibition prevents the pathway from expressing ltp. acute neurophysiological experiments were carried out in uret ... | 1990 | 2364294 |
| the cytolysin gene of vibrio vulnificus: sequence and relationship to the vibrio cholerae e1 tor hemolysin gene. | a cytolysin of ca. 56 kilodaltons has been suggested as a possible virulence factor in vibrio vulnificus infections. we sequenced the dna encoding cytolytic activity and found that the sequence contained two open reading frames, vvha and vvhb. vvha encoded the structural gene for the cytolysin and contained the n-terminal amino acid sequence previously reported for the protein. regions of the vvha gene showed homology to the structural gene for the vibrio cholerae e1 tor hemolysin. | 1990 | 2370116 |
| vibrio bacteremia in siriraj hospital. | from january 1983 to march 1988, 26 isolates of vibrio spp. were recovered from the blood of patients admitted to siriraj hospital. thirteen strains were identified as non 0-1 vibrio cholerae, 3 were vibrio vulnificus and 10 were vibrio spp. the medical records of 20 patients were available for clinical analysis. most of them were adult men with cirrhosis. clinical features included fever, abdominal pain, diarrhea, peritonitis, shock and skin lesions. some patients had a history of seafood consu ... | 1990 | 2380645 |
| white noise analysis of temporal properties in simple receptive fields of cat cortex. | we studied the linear and nonlinear temporal response properties of simple cells in cat visual cortex by presenting at single positions in the receptive field an optimally oriented bar stimulus whose luminance was modulated in a random, binary fashion. by crosscorrelating a cell's response with the input it was possible to obtain the zeroth-, first-, and second-order wiener kernels at each rf location. simple cells showed pronounced nonlinear temporal properties as revealed by the presence of pr ... | 1990 | 2390534 |
| increase in carcinoembryonic antigen release from cancer cells by combined treatment with retinoic acid and low-temperature hyperthermia. | the growth of a human gastric adenocarcinoma cell line, mkn-45, was inhibited and the amount of carcinoembryonic antigen (cea) in both the culture medium and the cell extract was increased in the presence of retinoic acid at a concentration of 75 (1.5x)-125 microm (1.9x), which did not substantially affect cell survival. treatment using a combination of retinoic acid (125 microm) and low-temperature hyperthermia (40 degrees c, 30 min) was more effective in increasing cea compared with retinoic a ... | 1990 | 2394926 |
| are separable aromatase systems involved in hormonal regulation of the male brain? | in vitro study of testosterone (t) metabolism shows that formation of estradiol-17 beta (e2) is regionally specific within the preoptic area (poa) of the male ring dove. the poa is known to be involved in the formation of e2 required for specific components of male sexual behavior. two sub-areas of high aromatase activity, anterior (apoa) and posterior preoptic (ppoa) areas, have been identified. aromatase activity is higher in apoa than in ppoa. the aromatase activity within the apoa is also mo ... | 1990 | 2394989 |
| metabolism of indium-111-labeled murine monoclonal antibody in tumor and normal tissue of the athymic mouse. | we have studied the fate of radiometal metabolism in vivo by analyzing the molecular form of radiolabeled anti-carcinoembryonic antigen (cea) in tissues. athymic mice bearing colonic tumor xenografts were injected i.v. with 111in-isothiocyanate-benzyl-dtpa-(scn-bz-dtpa) igg or 111in-(scn-bz-dtpa) f(ab')2, and then killed daily for up to four days. liver, kidney, and tumor were extracted and the supernatants, plasma and urine samples were analyzed by hplc, itlc, hpiec and sds-page. by hplc, the a ... | 1990 | 2395019 |
| structure-activity relationship of yohimbine and its related analogs in blocking alpha-1 and alpha-2 adrenoceptors: a comparative study of cardiovascular activities. | we investigated the selectivities and structure requirements for alpha-1 and alpha-2 adrenoceptor blocking activities of yohimbine (yo) and its 12 related analogs, such as beta-yohimbine (beta-yo), dihydrocorynantheine (dhc) and (-)indoloquinolizidine ((-)iq). the affinity of yo analogs to alpha-adrenoceptor was assessed by measuring their blockade of pressor responses to epinephrine in pithed rats. among yo structure groups, the potency order was yo greater than dhc = beta-yo greater than geiss ... | 1990 | 1976443 |
| cytolytic action of vibrio vulnificus haemolysin on mast cells from rat peritoneal cavity. | the mode of action of vibrio vulnificus haemolysin (vvh) on mast cells from the peritoneal cavity of the rat was examined. vvh induced histamine release, and damage to the mast cells, in a dose-dependent fashion. when 1 microgram of vvh was added to c. 10(5) mast cells at 37 degrees c, histamine release was observed after a lag period of 5-10 s, and was complete within 5 min. the action was temperature-dependent, and was not induced at 4 degrees c. disodium cromoglycate, a membrane stabiliser fo ... | 1990 | 1692881 |
| studies on the lipopolysaccharide of a virulent and an avirulent strain of vibrio vulnificus. | vibrio vulnificus is a marine bacterium associated with both primary septicemias and wound infections in humans. the lipopolysaccharides of a virulent and an avirulent strain of vibrio vulnificus were compared with respect to their chemical constituents and electrophoretic characteristics. 2-keto-3-deoxyoctonic acid, a normal constituent of the lipopolysaccharide of typical enterobacteriaceae, was not found in the lipopolysaccharide of either strain. hexadecenoate (c16:1) was the predominant fat ... | 1990 | 1693085 |
| developmental cycle-specific host-free rna synthesis in chlamydia spp. | the incorporation of radiolabeled gtp into rna in host-free chlamydia trachomatis serovar l2 organisms was investigated. the incorporation was partially inhibited by rifampin and dactinomycin and hydrolyzed by rnase. rna made by host-free chlamydiae consisted mainly of species of fewer than 800 bases in size, although 16s and 23s species were noted by agarose-gel electrophoresis. the hybridization of radiolabeled host-free rna to restriction fragments of the gene encoding the major outer membran ... | 1990 | 1698176 |
| variants of mta, the mouse maternally transmitted antigen, determined by three alleles of the extrachromosomal gene mtf. | two new forms of mta, the maternally transmitted antigen of the mouse, were defined by cytotoxic t lymphocytes. one is found in laboratory mice carrying the beta allele of the maternally transmitted factor, mtf, which were previously thought to be mta-negative. the other is associated with a new allele, mtf gamma, and is found in wild mice from toulouse. like the common form of mta, determined by the extrachromosomal mtf alpha, the new forms are also dependent on the a allele of hmt, a chromosom ... | 1990 | 1698881 |
| inhibitory effect of alpha 2-macroglobulin on vibrio vulnificus protease. | vibrio vulnificus, an etiologic agent of wound infections and septicemia in humans, elaborates a metalloprotease which is known to be an important virulence factor of the vibrio. the proteolytic activity of v. vulnificus metalloprotease (vvp) toward casein and elastin was inhibited by alpha 2-macroglobulin (alpha 2 m) at the molar ratio of 1:1, although partial activity was maintained. permeability-enhancing and hemorrhagic activities were also inhibited, but the peptidase activity toward z-gly- ... | 1989 | 2478526 |
| differential immunohistochemical localization of cytokeratins and collagen types i and iii in experimentally-induced cirrhosis. | liver cirrhosis was induced in male wistar rats by subcutaneous injection (1 ml of 30 g/l) of an aqueous solution of thioacetamide. using the indirect immunoperoxidase technique, high molecular weight keratins were localized in bile ducts and ductules. low molecular weight cytokeratins were present in regenerating hepatocytes in active cirrhosis; bile ducts were unstained. these results suggest that cytokeratin staining may be useful in distinguishing bile duct epithelium and hepatocytes in hepa ... | 1989 | 2478687 |
| sugar composition of the polysaccharide portion of lipopolysaccharides of vibrio fluvialis, vibrio vulnificus, and vibrio mimicus. | a chemotaxonomic study was carried out on vibrio fluvialis and v. vulnificus on the basis of the sugar composition of the polysaccharide portion of their lipopolysaccharides (lps). a previously developed rapid method of preparing samples for compositional sugar analysis was employed. nineteen o-serogroups of v. fluvialis were divided into 14 chemotypes while seven o-serogroups of v. vulnifucus were divided also into seven chemotypes since the polysaccharide portion of lps of each serogroup has a ... | 1989 | 2482433 |
| hemochromatosis and infection: alcohol and iron, oysters and sepsis. | hemochromatosis, or primary iron overload, is a variably expressed genetic metabolic disorder greatly modified by sex, age, diet, and alcohol consumption. although a diagnosis has been made at the bedside by careful documentation of the slow resolution of subcutaneous iron pigment, clinical diagnosis is frequently overlooked, and even autopsy may fail to reveal hemochromatosis as the cause for cirrhosis. genetic linkage studies have confirmed the extremely high prevalence of this disorder. untre ... | 1989 | 2486533 |
| immunocytochemical study of localization and traffic of thyroid peroxidase/microsomal antigen. | we studied the distribution of binding sites for anti-peroxidase monoclonal antibody and anti-microsomal antibodies on isolated human thyroid follicles and a human thyroid cell line. both open follicles and cells were incubated first with antibodies at +4 degrees c, then with colloidal gold labelled protein a. the topography of the binding sites for monoclonal anti-peroxidase antibody corresponded closely to the expected cell surface distribution of endogenous thyroid peroxidase since labelling ... | 1989 | 2491623 |
| unproven methods of cancer management. international association of cancer victors and friends, inc. | after study of the literature and other available information, the american cancer society has found no evidence that methods of treatment promoted by the international association of cancer victors and friends, inc. (iacvf), would result in objective benefit in the treatment of cancer in human beings. lacking such evidence, the american cancer society strongly urges individuals with cancer not to seek treatment based on the recommendations of iacvf. | 1989 | 2492878 |
| hemocyte monophenol oxidase activity in mosquitoes exposed to microfilariae of dirofilaria immitis. | monophenol oxidase (mpo) activity in hemocytes collected from aedes aegypti liverpool strain and aedes trivittatus intrathoracically inoculated with saline alone, inoculated with dirofilaria immitis microfilariae (mff), or from uninoculated mosquitoes was compared using a radiometric tyrosine hydroxylation assay. hemocyte mpo activity in mff-inoculated (= immune-activated) mosquitoes was significantly increased at 24 hr postinoculation (pi) in a. aegypti and at 6, 12, and 24 hr pi in a. trivitta ... | 1989 | 2493084 |
| activation of hageman factor and prekallikrein and generation of kinin by various microbial proteinases. | activation of the hageman factor-kallikrein-kinin system by serratial 56-kda proteinase was previously demonstrated (matsumoto, k., yamamoto, t., kamata, t., and maeda, h. (1984) j. biochem. (tokyo) 96, 739-749; kamata, r., yamamoto, t., matsumoto, k., and maeda, h. (1985) infect. immun. 48, 747-753). to investigate whether the activation of the system is specific for 56-kda proteinase or is found similarly with other microbial proteinases, 11 proteinases of microbial origins were studied; the 5 ... | 1989 | 2499581 |
| [two case reports of septic shock due to vibrio vulnificus with liver cirrhosis]. | we have recently experienced a case of vibrio vulnificus septicemia which occurred in a patient with hepatic cirrhosis, and as we were able to give early antibiotic treatment, the patient survived. we would like to report this case here together with another case experienced 2 years ago. case 1 was a 58-year-old male who was attending our hospital as an outpatient for hepatic cirrhosis. at 5:30 pm on august 8, 1987, he consumed abalone and giant clam and at 9 pm complained of high fever with sha ... | 1989 | 2501432 |
| killing of human tumor cells by antibody c3b conjugates and human complement. | to potentiate the lytic action of complement on tumor cells, we have constructed heteroconjugates composed of monoclonal antibody and of the human c3b component of complement. the conjugates were formed efficiently using the heterobifunctional cross-linking reagent spdp. the monoclonal antibody-c3b conjugate promoted the killing of k562 tumor cells by normal human serum. treatment of the tumor cells with the monoclonal antibody and normal human serum resulted in 10-15% lysis. however, following ... | 1989 | 2519543 |