Publications

jinggangmycin increases fecundity of the brown planthopper, nilaparvata lugens (stål) via fatty acid synthase gene expression.	the antibiotic jinggangmycin (jgm) is mainly used in controlling the rice sheath blight, rhizoctonia solani, in china. jgm also enhances reproduction of the brown planthopper (bph), nilaparvata lugens (stål). to date, however, molecular mechanisms of the enhancement are unclear. our related report documented the influence of foliar jgm sprays on ovarian protein content. here, we used isobaric tags for relative and absolute quantitation (itraq) protocols to analyze ovarian proteins of bph females ...	2016	26388431
molecular characterization, tissue and developmental expression profiles of cryptochrome genes in wing dimorphic brown planthoppers, nilaparvata lugens.	cryptochromes (crys) are blue and uv light photoreceptors, known to play key roles in circadian rhythms and in the light-dependent magnetosensitivity of insects. two novel cryptochrome genes were cloned from the brown planthopper, and were given the designations of nlcry1 and nlcry2, with the accession numbers km108578 and km108579 in genbank. the complementary dna sequences of nlcry1 and nlcry2 are 1935 bp and 2463 bp in length, and they contain an open reading frame of 1629 bp and 1872 bp, enc ...	2016	26227859
antifeedants of indian barnyard millet, echinochloa frumentacea link, against brown planthopper, nilaparvata lugens (stål).	eight compounds isolated from indian barnyard millet have been identified as l-malic acid, trans-aconitic acid, (+)-isocitric acid, 5-o-caffeoylquinic acid, 4-o-caffeoylquinic acid, isocarlinoside, 2"-o-rhamnosylisoorientin, and 7-o-(2"-o-glucuronosyl)glucuronosyltricin, respectively. these compounds showed high antifeeding activity against brown planthopper only when they were combined.	2016	19040117
probing stimulants from the rice plant towards the smaller brown planthopper, laodelphax striatellus (fallen) (homoptera: delphacidae).	when adult males of the smaller brown planthopper, laodelphax striatellus were fed on 2% crude rice (leaf and stem) extract containing 15% sucrose there were characteristic stylet sheaths deposited on parafilm membrane. further bioassays with the butanol-soluble fr. of the extract revealed that it is highly effective for the insects. when the butanol fr. was charged on an ods open column and eluted in sequence with 20, 40 and 100% methanol in water, the ods-40% methanol fr. was shown as the most ...	2015	11204184
herbivory-induced glucose transporter gene expression in the brown planthopper, nilaparvata lugens.	nilaparvata lugens, the brown planthopper (bph) feeds on rice phloem sap, containing high amounts of sucrose as a carbon source. nutrients such as sugars in the digestive tract are incorporated into the body cavity via transporters with substrate selectivity. eighteen sugar transporter genes of bph (nlst) were reported and three transporters have been functionally characterized. however, individual characteristics of nlst members associated with sugar transport remain poorly understood. comparat ...	2015	26226652
construction of an immobilised acetylcholinesterase column and its application in screening insecticidal constituents from magnolia officinalis.	application of a matrix-immobilised target enzyme for screening inhibitors is widely used in drug development, but there are few studies in insecticide discovery. in this paper, an economical and effective immobilised acetylcholinesterase (ache) column was prepared using the sol-gel embedment method, which was further combined with high-performance liquid chromatography for screening the ache inhibitors and insecticidal compounds from complex natural products.	2015	25228142
chitinase-like gene family in the brown planthopper, nilaparvata lugens.	chitinases are important enzymes required for chitin degradation and reconstruction in insects. based on a bioinformatics investigation, we identified 12 genes encoding putative chitinase-like proteins, including 10 chitinases (cht), one imaginal disc growth factor (idgf) and one endo-β-n-acetylglucosaminidase (engase) in the genome of the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). these 12 genes were clustered into nine different groups, with 11 in glycoside hydrolase famil ...	2015	25224926
changes in endosymbiotic bacteria of brown planthoppers during the process of adaptation to different resistant rice varieties.	the specific primers of five species of endosymbiotic bacteria were designed to determine their numbers in three virulent populations of brown planthopper, nilapavata lugens stål, and to assess changes during adaptation to different resistant varieties using fluorescent quantitative pcr. the results showed that chryseobacterium was the dominant bacteria in all three populations of brown planthopper, followed by acinetobacter in tn1 population, arsenophonus and serratia in mudgo population, and a ...	2015	26313963
the β-n-acetylhexosaminidase gene family in the brown planthopper, nilaparvata lugens.	β-n-acetylhexosaminidases (hexs) are enzymes that can degrade the chitin oligosaccharides that are produced by the activity of chitinases on chitin in insects. using bioinformatic methods based on genome and transcriptome databases, 11 β-n-acetylhexosaminidase genes (nlhexs) in nilaparvata lugens were identified and characterized. phylogenetic analysis revealed a six-grouped tree topology. the o-linked n-acetylglucosaminidase (o-glcnacase) group includes nlhex11, which harbours a catalytic domai ...	2015	26304035
reduction in mrna and protein expression of a nicotinic acetylcholine receptor α8 subunit is associated with resistance to imidacloprid in the brown planthopper, nilaparvata lugens.	target-site resistance is commonly caused by qualitative changes in insecticide target-receptors and few studies have implicated quantitative changes in insecticide targets in resistance. here we show that resistance to imidacloprid in a selected strain of nilaparvata lugens is associated with a reduction in expression levels of the nicotinic acetylcholine receptor (nachr) subunit nlα8. synergism bioassays of the selected strain suggested resistance was conferred, in part, by a target-site mecha ...	2015	26259922
genomic and transcriptomic insights into the cytochrome p450 monooxygenase gene repertoire in the rice pest brown planthopper, nilaparvata lugens.	the cytochrome p450 monooxygenase (p450) gene family is one of the most abundant eukaryotic gene families that encode detoxification enzymes. in this study, we identified an abundance of p450 gene repertoire through genome- and transcriptome-wide analysis in the brown planthopper (nilaparvata lugens), the most destructive rice pest in asia. detailed gene information including the exon-intron organization, size, transcription orientation and distribution in the genome revealed that many p450 loci ...	2015	26234643
functional screen for micrornas of nilaparvata lugens reveals that targeting of glutamine synthase by mir-4868b regulates fecundity.	insect fecundity is regulated by the interaction of genotypes and the environment. micrornas (mirnas) also act in insect development and reproduction by regulating genes involved in these physiological processes. although hundreds of insect mirnas have been identified, the biological roles of most remain poorly understood. here, we used a multi-algorithm approach for mirna target prediction in 3'utrs of fecundity-related genes in the brown planthopper (bph) nilaparvata lugens and identified 38 p ...	2015	26546713
a salivary sheath protein essential for the interaction of the brown planthopper with rice plants.	salivary secretions, including gel saliva and watery saliva, play crucial roles in the interaction between the insect and plant during feeding. in this study, we identified a salivary gland-specific gene encoding a salivary sheath protein (nlshp) in nilaparvata lugens. nlshp has two alternative splicing variants; both are expressed at high levels during the nymph and adult stages. immunohistochemical staining showed that the nlshp were synthesized in the principal gland cells of the salivary gla ...	2015	26483289
intraspecific and interspecific variations in the mitochondrial genomes of nilaparvata (hemiptera: delphacidae).	planthoppers in the genus nilaparvata distant are serious pests of rice and many other crops in tropical and temperate asia, and northern australia. in this study, the mitochondrial genomes of four nilaparvata planthoppers were sequenced, three in nilaparvata lugens stål and one in nilaparvata muiri china. mitochondrial genome of nilaparvata contain the standard set of 13 protein-coding genes, 22 transfer rna genes, 2 ribosomal rna genes, and a control region. the nucleotide composition of nilap ...	2015	26470349
analysis of the time-concentration-mortality response of nilaparvata lugens (hemiptera: delphacidae) to paichongding.	paichongding is an adicyclicnitromethylene neonicotinoid insecticide with a cis-configuration. bioassay of paichongding was conducted against nilaparvata lugens stål under a laboratory condition. mortality of n. lugens was analyzed by time-concentration-mortality (tcm) regressions based on the complementary log-log (cll) model. the conditional mortalities of test individuals increased with the exposure time after treatments with different concentrations, showing that the speed of insecticidal ac ...	2015	26470347
a comparative study on the population fitness of three strains of nilaparvata lugens (hemiptera: delphacidae) differ in eye color-related genes.	the brown planthopper, nilaparvata lugens (stål), is a destructive insect pest on rice throughout asia. as a visible genetic marker, red eye mutant colony of brown planthopper is a valuable material. here, we established the near-isogenic lines, nil-bb and nil-rr, through mating red eye females to brown eye brothers for eight successive generations. biological experiments showed that nil-bb had big fitness cost; however, nil-rr had comparable survival and fertility parameters with bb, a normal l ...	2015	26470308
differentially regulated genes in the salivary glands of brown planthopper after feeding in resistant versus susceptible rice varieties.	brown planthopper (bph) is a damaging insect pest of rice. we used suppression subtractive hybridization (ssh) and mirror orientation selection to identify differentially regulated genes in salivary glands of bph after feeding on resistant and susceptible varieties. the forward ssh library included 768 clones with insertions ranging from 250 to 1000 bp. after differential screening, a total of 112 transcripts were identified, which included 27 upregulated genes and seven downregulated genes. sev ...	2015	25611813
the genetic basis of population fecundity prediction across multiple field populations of nilaparvata lugens.	identifying the molecular markers for complex quantitative traits in natural populations promises to provide novel insight into genetic mechanisms of adaptation and to aid in forecasting population dynamics. in this study, we investigated single nucleotide polymorphisms (snps) using candidate gene approach from high- and low-fecundity populations of the brown planthopper (bph) nilaparvata lugens stål (hemiptera: delphacidae) divergently selected for fecundity. we also tested whether the populati ...	2015	25581109
structural characterization of the catalytic site of a nilaparvata lugens delta-class glutathione transferase.	glutathione transferases (gsts) are a major class of detoxification enzymes that play a central role in the defense against environmental toxicants and oxidative stress. here, we studied the crystal structure of a delta-class glutathione transferase from nilaparvata lugens, nlgstd, to gain insights into its catalytic mechanism. the structure of nlgstd in complex with glutathione, determined at a resolution of 1.7å, revealed that it exists as a dimer and its secondary and tertiary structures are ...	2015	25497345
the effects of triazophos applied to transgenic bt rice on the nutritional indexes, nlvg expression, and population growth of nilaparvata lugens stål under elevated co₂.	the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae), is a typical pest in which population resurgence can be induced by insecticides. warmer global temperatures, associated with anthropogenic climate change, are likely to have marked ecological effects on terrestrial ecosystems. however, the effects of elevated co2 (eco2) concentrations on the resurgence of n. lugens that have been treated with pesticides used for transgenic bt rice cultivation are not fully understood. the ...	2015	25752430
a new finely mapped oryza australiensis-derived qtl in rice confers resistance to brown planthopper.	brown planthopper (bph) is the most destructive pest of rice in asia. the bph resistance in the introgression line ir65482-17-511-5-7 (ir65482-17) is derived from the wild rice species oryza australiensis. an f2:3 population from a cross between zhenshan 97 (zs97) and ir65482-17 was used to map three quantitative trait loci (qtls) for seedling resistance and feeding rate to bph. the loci were distributed on chromosomes 2, 4 and 12. the qtl qbph4.2 on chromosome 4 had the largest effect, and cont ...	2015	25682936
molecular characterization and rna interference analysis of vitellogenin receptor from nilaparvata lugens (stål).	vitellogenin receptors (vgrs), members of the low-density lipoprotein receptor (ldlr) superfamily, are responsible for taking vitellogenin (vg) into developing oocytes. here the first full-length vgr cdna from a hemipteran insect, the brown planthopper (nilaparvata lugens), was cloned and sequenced. the complete mrna sequence was 6174 bp in length with an open reading frame (orf) of 5796 bp encoding 1931 amino acid residues. n. lugens vgr (nlvgr) contained two ligand-binding domains with five ld ...	2015	25617689
silencing a sugar transporter gene reduces growth and fecundity in the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae).	the brown planthopper (bph), nilaparvata lugens, sugar transporter gene 6 (nlst6) is a facilitative glucose/fructose transporter (often called a passive carrier) expressed in midgut that mediates sugar transport from the midgut lumen to hemolymph. the influence of down regulating expression of sugar transporter genes on insect growth, development, and fecundity is unknown. nonetheless, it is reasonable to suspect that transporter-mediated uptake of dietary sugar is essential to the biology of ph ...	2015	26185058
the multicopper oxidase gene family in the brown planthopper, nilaparvata lugens.	the multicopper oxidase (mco) family of enzymes includes laccases, ascorbate oxidases, bilirubin oxidases and a subgroup of metal oxidases. on the basis of a bioinformatics investigation, we identified 7 genes encoding putative multicopper oxidase proteins in the genome of the brown planthopper (bph), nilaparvata lugens (hemiptera: delphacidae). mco1 and mco2 are conserved, while others diverse in insects. analysis of developmental and tissue-specific expression patterns revealed the following: ...	2015	26107750
dna methyltransferases have an essential role in female fecundity in brown planthopper, nilaparvata lugens.	dna methylation is an ancient epigenetic modification present in all three domains of life. however, the understanding of dna methylation in insects is limited. here, we amplified the full-length transcripts of the dna methyltransferases nlu-dnmt1 and nlu-dnmt3, indicating that a complete dna methylation toolkit exists in the brown planthopper, nilaparvata lugens, a destructive pest in rice production. nlu-dnmt1 and nlu-dnmt3 had the conserved motifs and domains of the dna methyltransferase fami ...	2015	26043687
activation of the tor signalling pathway by glutamine regulates insect fecundity.	the target of rapamycin (tor) positively controls cell growth in response to nutrients such as amino acids. however, research on the specific nutrients sensed by tor is limited. glutamine (gln), a particularly important amino acid involved in metabolism in organisms, is synthesised and catalysed exclusively by glutamine synthetase (gs), and our previous studies have shown that gln may regulate fecundity in vivo levels of the brown planthopper (bph) nilaparvata lugens. until now, it has remained ...	2015	26024507
two insulin receptors determine alternative wing morphs in planthoppers.	wing polyphenism is an evolutionarily successful feature found in a wide range of insects. long-winged morphs can fly, which allows them to escape adverse habitats and track changing resources, whereas short-winged morphs are flightless, but usually possess higher fecundity than the winged morphs. studies on aphids, crickets and planthoppers have revealed that alternative wing morphs develop in response to various environmental cues, and that the response to these cues may be mediated by develop ...	2015	25799997
evidence for high dispersal ability and mito-nuclear discordance in the small brown planthopper, laodelphax striatellus.	understanding dispersal ability in pest species is critical for both theoretical aspects of evolutionary and population biology and from a practical standpoint, such as implementing effective forecasting systems. the small brown planthopper (sbph), laodelphax striatellus (fallén), is an economically important pest, but few data exist on its dispersal ability. here, we used mitochondrial and nuclear markers to elucidate the population genetic structure of sbph and of the parasitic bacterium wolba ...	2015	25622966
molecular cloning and characterization of the putative halloween gene phantom from the small brown planthopper laodelphax striatellus.	ecdysteroid hormone 20-hydroxyecdysone plays fundamental roles in insect postembryonic development and reproduction. several cytochrome p450 mono-oxygenases (cyps), encoded by the halloween genes, have been documented to be involved in ecdysteroidogenesis in representative insects in diptera, lepidoptera and orthoptera. here the putative halloween gene phantom (phm, cyp306a1) from a hemipteran insect species, the small brown planthopper laodelphax striatellus, was cloned. lsphm shows five insect ...	2015	24954278
a halloween gene shadow is a potential target for rna-interference-based pest management in the small brown planthopper laodelphax striatellus.	laodelphax striatellus is an economically important rice pest in china. ecdysteroid hormone 20-hydroxyecdysone regulates insect development and reproduction. the cytochrome p450 monooxygenase shadow (sad) plays a critical role in ecdysteroidogenesis. here, tests were conducted to establish whether lssad was a potential target gene for rna-interference-based management of l. striatellus.	2015	24648012
phloem-exudate proteome analysis of response to insect brown plant-hopper in rice.	brown plant-hopper (nilaparvata lugens stål, bph), one of the most devastating agricultural insect pests of rice throughout asia, ingests nutrients from rice sieve tubes and causes a dramatic yield loss. planting resistant variety is an efficient and economical way to control this pest. understanding the mechanisms of host resistance is extremely valuable for molecular design of resistant rice variety. here, we used an itraq-based quantitative proteomics approach to perform analysis of protein e ...	2015	26072143
application of brown planthopper salivary gland extract to rice plants induces systemic host mrna patterns associated with nutrient remobilization.	insect saliva plays an important role in modulation of plant-insect interactions. although this area of research has generated much attention in recent years, mechanisms of how saliva affects plant responses remain poorly understood. to address this void, the present study investigated the impact of the brown planthopper (nilaparvata lugens, stål; hereafter bph) salivary gland extract (sge) on rice (oryza sativa) systemic responses at the mrna level. differentially expressed rice mrnas were gene ...	2015	26641488
identification of polymorphisms in cyrtorhinus lividipennis rdl subunit contributing to fipronil sensitivity.	as one of the most important predatory enemies, the miridbug, cyrtorhinus lividipennis, plays an important role in rice planthoppers control, such as nilaparvata lugens (brown planthopper). in order to compare insecticide selectivity between c. lividipennis and n. lugens, the contact acute toxicities of six insecticides (diazoxon, paraoxon, carbaryl, fenobucarb, fipronil and ethofenprox) were monitored. the results showed that all tested insecticides were more toxic to c. lividipennis than to n. ...	2015	25619913
identification of key amino acid differences between cyrtorhinus lividipennis and nilaparvata lugens nachr α8 subunits contributing to neonicotinoid sensitivity.	high sensitivity to neonicotinoid insecticides have been reported in the miridbug cyrtorhinus lividipennis, an important predatory enemy of rice planthoppers, such as nilaparvata lugens (brown planthopper). in the present study, the sensitivity of neonicotinoid insecticides between c. lividipennis and n. lugens were detected and compared. the results showed that neonicotinoid insecticides were much more toxic to the miridbug than to the brown planthopper. a nicotinic acetylcholine receptor subun ...	2015	25613467
knockdown of the ionotropic γ-aminobutyric acid receptor (gabar) rdl gene decreases fipronil susceptibility of the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae).	insect γ-aminobutyric acid receptors (gabars) are important molecular targets of cyclodiene and phenylpyrazole insecticides. previously gabars encoding rdl (resistant to dieldrin) genes responsible for dieldrin and fipronil resistance were identified in various economically important insect pests. in this study, we cloned the open reading frame cdna sequence of rdl gene from fipronil-susceptible and fipronil-resistant strains of laodelphax striatellus (lsrdl). sequence analysis confirmed the pre ...	2015	25808850
take-off time of the first generation of the overwintering small brown planthopper, laodelphax striatellus in the temperate zone in east asia.	overseas migration of the small brown planthopper, laodelphax striatellus (fallén), occurs during the winter wheat harvest season in east asia. knowing the take-off time of emigrating l. striatellus is crucial for predicting such migrations with a simulation technique because winds, carriers of migratory insects, change continuously. several methods were used in china and japan from late may to early june 2012 and again in 2013 to identify the precise timing of take-off. these methods included: ...	2015	25780936
omics-based comparative transcriptional profiling of two contrasting rice genotypes during early infestation by small brown planthopper.	the small brown planthopper (sbph) is one of the destructive pests of rice. although different biochemical pathways that are involved in rice responding to planthopper infestation have been documented, it is unclear which individual metabolic pathways are responsive to planthopper infestation. in this study, an omics-based comparative transcriptional profiling of two contrasting rice genotypes, an sbph-resistant and an sbph-susceptible rice line, was assessed for rice individual metabolic pathwa ...	2015	26633389
differential interactions of 5-(4-piperidyl)-3-isoxazolol analogues with insect γ-aminobutyric acid receptors leading to functional selectivity.	γ-aminobutyric acid (gaba) receptors (gabars) mediate fast inhibitory synaptic transmission and are also targets for drugs and insecticides. to better understand the molecular interactions of ligands with the orthosteric sites of gabars, we examined 4-aryl/arylalkyl-5-(4-piperidyl)-3-isoxazolol, 4-aryl-5-(4-piperidyl)-3-isothiazolol, and 5-aryl-4-(4-piperidyl)-1-hydroxypyrazole for their antagonism with regard to three insect gabars. the 3-isoxazolol was preferable to the 3-isothiazolol and 1-hy ...	2015	26453818
genetic basis of multiple resistance to the brown planthopper (nilaparvata lugens stål) and the green rice leafhopper (nephotettix cincticeps uhler) in the rice cultivar 'asd7' (oryza sativa l. ssp. indica).	the rice cultivar asd7 (oryza sativa l. ssp. indica) is resistant to the brown planthopper (bph; nilaparvata lugens stål) and the green leafhopper (nephotettix virescens distant). here, we analyzed multiple genetic resistance to bph and the green rice leafhopper (grh; nephotettix cincticeps uhler). using two independent f2 populations derived from a cross between asd7 and taichung 65 (oryza sativa ssp. japonica), we detected two qtls (qbph6 and qbph12) for resistance to bph and one qtl (qgrh5) f ...	2015	26719745
apterous a modulates wing size, bristle formation and patterning in nilaparvata lugens.	apterous a (apa), a member of the lim-homeobox gene family, plays a critical role in the development of wing. the achaete-scute complex (as-c) encodes basic helix-loop-helix (bhlh) transcription factors and functions in bristle development. in the present study, we cloned apa (nlapa) and an achaete-scute homologue (nlash) from n. lugens. levels of nlapa and nlash were higher in nymphs than adults, with particularly high expression in the thorax of nymphs. nlapa expressed more highly in nymphs of ...	2015	25995006
map-based cloning and characterization of bph29, a b3 domain-containing recessive gene conferring brown planthopper resistance in rice.	rice (oryza sativa l.) production, essential for global food security, is threatened by the brown planthopper (bph). the breeding of host-resistant crops is an economical and environmentally friendly strategy for pest control, but few resistance gene resources have thus far been cloned. an indica rice introgression line rbph54, derived from wild rice oryza rufipogon, has been identified with sustainable resistance to bph, which is governed by recessive alleles at two loci. in this study, a map-b ...	2015	26136269
prioritizing plant defence over growth through wrky regulation facilitates infestation by non-target herbivores.	plants generally respond to herbivore attack by increasing resistance and decreasing growth. this prioritization is achieved through the regulation of phytohormonal signaling networks. however, it remains unknown how this prioritization affects resistance against non-target herbivores. in this study, we identify wrky70 as a specific herbivore-induced, mitogen-activated protein kinase-regulated rice transcription factor that physically interacts with w-box motives and prioritizes defence over gro ...	2015	26083713
rna interference-mediated silencing of a halloween gene spookier affects nymph performance in the small brown planthopper laodelphax striatellus.	post-embryonic development of insects is highly dependent on ecdysteroid hormone 20-hydroxyecdysone. halloween gene spookier (spok, cyp307a2) has been documented to be involved in ecdysteroidogenesis in drosophila melanogaster and bombyx mori. we describe here the cloning and characterization of halloween gene spookier (lsspok, lscyp307a2) in the small brown planthopper laodelphax striatellus, a hemipteran insect species. lsspok has three insect-conserved p450 motifs, that is, helix-k, perf moti ...	2015	24282064
effects of host sex, plant species, and putative host species on the prevalence of wolbachia in natural populations of bemisia tabaci (hemiptera: aleyrodidae): a modified nested pcr study.	bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a globally distributed pest. one of the key endosymbionts in b. tabaci is wolbachia, an α-proteobacterium implicated in many important biological processes. previous studies indicated that the infection frequency of wolbachia in middle east-asia minor 1 (meam1) and mediterranean (med) varied greatly among populations in different areas. however, little is known about the factors that influence the prevalence of wolbachia in b. tabaci. in thi ...	2015	26470122
bicaudal-c plays a vital role in oogenesis in nilaparvata lugens (hemiptera: delphacidae).	bicaudal-c (bic-c) was originally identified in a drosophila melanogaster mutagenesis screen and plays vital roles in embryogenesis. in this study, we characterized the bic-c gene in the brown planthopper, nilaparvata lugens (hemiptera: delphacidae), an insect pest that undergoes incomplete metamorphosis. our result showed that n. lugens bic-c (nlbic-c) is a female-specific gene in this species. it is specifically expressed in developing oocytes and is not expressed in laid eggs. ribonucleic aci ...	2015	26025198
differential mechanisms of action of the novel γ-aminobutyric acid receptor antagonist ectoparasiticides fluralaner (a1443) and fipronil.	fluralaner (a1443) is an isoxazoline ectoparasiticide that is a novel antagonist of γ-aminobutyric acid (gaba) receptors (gabars), with a potency comparable to that of fipronil, a phenylpyrazole ectoparasiticide. to clarify the biological effectiveness of fluralaner against fipronil-resistant pests, differences in the actions of fluralaner and fipronil on gabars that possess resistance to dieldrin (rdl)-type mutations were evaluated.	2015	24591229
infection rate assay by nested pcr and the phylogenetic analysis of himetobi p virus in the main pests of rice-wheat cropping systems.	himetobi p virus (hipv) is an ssrna in the family dicistroviridae that infects rice pests belonging to hemiptera. to determine its host range, a nested pcr method was designed to detect hipv in some of the main rice pests (hemiptera) in eastern china. the incidence of infection in the grain aphid sitobion avenae fabricius (hemiptera: aphididae) was low (3%), while high incidences of infection occurred in the planthoppers laodelphax striatellus (fallén) (hemiptera: delphacidae) (100%) and nilapar ...	2015	26470259
genetic analysis and molecular mapping of qtls for resistance to rice black-streaked dwarf disease in rice.	rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (rbsdv), is transmitted by small brown planthoppers (laodelphax striatellus fallén, sbph) and causes severe yield loss in epidemic years in china and other east asian countries. breeding for resistance to rbsdv is a promising strategy to control the disease. we identified tetep that showed resistance to rbsdv using a field test and artificial inoculation test. an evaluation of the resistance mechanism revealed that tete ...	2015	26198760
whole-genome expression analysis of rice black-streaked dwarf virus in different plant hosts and small brown planthopper.	rice black-streaked dwarf virus (rbsdv) can infect a number of gramineous plants and cause severe crop yield losses in southeast asian countries. the virus is transmitted by small brown planthopper (sbph) in a persistent circulative manner. the interactions between rbsdv and its different hosts remain unknown. besides, how the virus adjusts itself to infect different hosts is unclear. in the present study, the relative rna levels of the thirteen rbsdv genes in rice, maize, wheat, and sbph were m ...	2015	26149652
quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time.	southern rice black streaked dwarf virus (srbsdv) and rice black streaked dwarf virus (rbsdv) are serious rice-infecting reoviruses, which are transmitted by different planthoppers in a persistent propagative manner. in this study, we quantitatively compared the spatial distribution of srbsdv and rbsdv contents over time in their vector and nonvector insects using real time-pcr. genome equivalent copies (gec) were assessed every 2 days from 0 to 14 days after a 3-days acquisition access period ( ...	2015	26116274
a simplified method for simultaneous detection of rice stripe virus and rice black-streaked dwarf virus in insect vector.	rice stripe virus (rsv) and rice black-streaked dwarf virus (rbsdv) are transmitted by their common vector small brown planthopper (sbph) that cause serious crop losses in china. a simple reverse transcription-pcr method was developed for the simultaneous detection of rsv and rbsdv in single sbph. three primers targeted to rsv-rna4 and rbsdv-s2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. the method is reliable, rapid and inexpensive for detecting th ...	2015	25455902
tetrahydroindeno[1',2':4,5]pyrrolo[1,2-a]imidazol-5(1h)-ones as novel neonicotinoid insecticides: reaction selectivity and substituent effects on the activity level.	tetraheterocyclic tetrahydroindeno[1',2':4,5]pyrrolo[1,2-a]imidazol-5(1h)-one derivatives as novel neonicotinoid candidates were designed and prepared by selective etherification, chlorination and esterification of ninhydrin-heterocyclic ketene aminals adducts. some of the designed compounds showed excellent insecticidal activity against cowpea aphids (aphis craccivora), brown planthopper (nilaparvata lugens), and armyworm (mythimna separata). in particular, the activity against armyworm (mythim ...	2015	25611859
molecular cloning and expression profile of an atp-binding cassette (abc) transporter gene from the hemipteran insect nilaparvata lugens.	the atp-binding cassette (abc) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. in insects, abc transporters have important functions in the transport of molecules, and are also involved in insecticide resistance, metabolism, and development. in this study, the nilaparvata lugens stal (hemiptera: delphacidae) abcg (nlabcg) gene was identified and characterized. the complete mrna sequence of nlabcg was 2608-bp long, with a ...	2015	25867414
rna interference of nadph-cytochrome p450 reductase of the rice brown planthopper, nilaparvata lugens, increases susceptibility to insecticides.	nadph-cytochrome p450 reductase (cpr) is essential for numerous biological reactions catalysed by microsomal cytochrome p450 monooxygenases (p450s). knockdown of cpr in several insects leads to developmental defects and increased susceptibility to insecticides. however, information about the role of cpr in the brown planthopper, nilaparvata lugens, is still unavailable.	2015	24515640
molecular analysis of oryza latifolia desv. (ccdd genome)-derived introgression lines and identification of value-added traits for rice (o. sativa l.) improvement.	oryza latifolia is a tetraploid wild oryza species with a ccdd genome that has been reported to harbor resistance to bacterial blight (bb), brown planthopper, and whitebacked planthopper. aside from these traits, o. latifolia is also being tapped as a new source of resistance to lodging and high biomass production. to explore the genetic potential of o. latifolia as a novel genetic resource for the improvement of existing o. sativa cultivars, 27 disomic derivatives of o. latifolia monosomic alie ...	2015	24939891
genomic analysis of an ascomycete fungus from the rice planthopper reveals how it adapts to an endosymbiotic lifestyle.	a number of sap-sucking insects harbor endosymbionts, which are thought to play an important role in the development of their hosts. one of the most important rice pests, the brown planthopper (bph), nilaparvata lugens (stål), harbors an obligatory yeast-like symbiont (yls) that cannot be cultured in vitro. genomic information on this yls would be useful to better understand its evolution. in this study, we performed genome sequencing of the yls using both 454 and illumina approaches, generating ...	2015	26338189
rna interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae).	the brown planthopper nilaparvata lugens is a serious phloem-feeding pest of rice in china. the current study focuses on a saccharopine dehydrogenase (sdh) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (lys), which plays a role in insect growth and carnitine production (as a substrate). the protein, provisionally designated as nlylssdh [a sdh derived from yeast-like symbiont (yls) in n. lugens], had a higher transcript level in abdomens, compared with heads, wi ...	2015	25908053
pichia anomala, a new species of yeast-like endosymbionts and its variation in small brown planthopper (laodelphax striatellus).	yeast-like symbionts (yls) are endosymbionts that promote the growth of delphacid planthoppers (hemiptera: delphacidae), some of which are pests on cultivated rice. identification and characterization of yls growth can be helpful for pest control, because it has been demonstrated that there is a variety of yls in rice planthopper and they affected the planthopper's growth and virulence to plant hosts. so, elucidation of the species of yls in planthopper is crucial for exploiting a new way to con ...	2015	25499750
rice stripe tenuivirus nonstructural protein 3 hijacks the 26s proteasome of the small brown planthopper via direct interaction with regulatory particle non-atpase subunit 3.	the ubiquitin/26s proteasome system plays a vital role in regulating host defenses against pathogens. previous studies have highlighted different roles for the ubiquitin/26s proteasome in defense during virus infection in both mammals and plants, but their role in the vectors that transmit those viruses is still unclear. in this study, we determined that the 26s proteasome is present in the small brown planthopper (sbph) (laodelphax striatellus) and has components similar to those in plants and ...	2015	25653432
small interfering rna pathway modulates initial viral infection in midgut epithelium of insect after ingestion of virus.	numerous viruses are transmitted in a persistent manner by insect vectors. persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. although propagation of viruses in insect vectors can be controlled by the small interfering rna (sirna) antiviral pathway, whether the sirna pathway can control viral dissemination from the midgut epithelium is unknown. infection by a rice virus (southern rice black streaked dwarf vir ...	2015	26537672
rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos.	plant viruses may affect the viability and development process of their herbivore vectors. small brown planthopper (sbph) is main vector of rice stripe virus (rsv), which causes serious rice stripe disease. here, we reported the effects of rsv on sbph offspring by crossing experiments between viruliferous and non-viruliferous strains. the life parameters of offspring from different cross combinations were compared. the hatchability of f1 progeny from viruliferous parents decreased significantly, ...	2015	25601039
facilitation of rice stripe virus accumulation in the insect vector by himetobi p virus vp1.	the small brown planthopper (sbph) is the main vector for rice stripe virus (rsv), which causes serious rice stripe disease in east asia. to characterize the virus-vector interactions, the sbph cdna library was screened with rsv ribonucleoprotein (rnp) as bait using a gal4-based yeast two-hybrid system. the interaction between rsv-rnp and the himetobi p virus (hipv, an insect picorna-like virus) vp1 protein was identified. the relationships between hipv and rsv in sbph were further investigated, ...	2015	25807055
proteomic analysis of interaction between a plant virus and its vector insect reveals new functions of hemipteran cuticular protein.	numerous viruses can be transmitted by their corresponding vector insects; however, the molecular mechanisms enabling virus transmission by vector insects have been poorly understood, especially the identity of vector components interacting with the virus. here, we used the yeast two-hybrid system to study proteomic interactions of a plant virus (rice stripe virus, rsv, genus tenuivirus) with its vector insect, small brown planthopper (laodelphax striatellus). sixty-six proteins of l. striatellu ...	2015	26091699
pseudo-backcrossing design for rapidly pyramiding multiple traits into a preferential rice variety.	pyramiding multiple genes into a desirable genetic background can take years to accomplish. in this paper, a pseudo-backcrossing scheme was designed to shorten the backcrossing cycle needed. pink3, an aromatic and potentially high-yielding rice variety-although one that is intolerant to flash flooding (sub) and susceptible to bacterial leaf blight (bb), leaf-neck blast (bl) and the brown planthopper (bph)-was used as a genetic basis for significant improvements through gene pyramiding.	2015	25844112
identification and function analysis of enolase gene nleno1 from nilaparvata lugens (stål) (hemiptera:delphacidae).	the enolase [ec 4.2.1.11] is an essential enzyme in the glycolytic pathway catalyzing the conversion of 2-phosphoglycerate (2-pge) to phosphoenolpyruvate (pep). in this study, a full-length cdna encoding α-enolase was cloned from rice brown planthopper (nilaparvata lugens) and is provisionally designated as nleno1. the cdna sequence of nleno1 was 1,851 bp with an open reading frame (orf) of 1,305 bp and encoding 434 amino acids. the deduced protein shares high identity of 80-87% with eno1-like p ...	2015	26056319
insectbase: a resource for insect genomes and transcriptomes.	the genomes and transcriptomes of hundreds of insects have been sequenced. however, insect community lacks an integrated, up-to-date collection of insect gene data. here, we introduce the first release of insectbase, available online at http://www.insect-genome.com. the database encompasses 138 insect genomes, 116 insect transcriptomes, 61 insect gene sets, 36 gene families of 60 insects, 7544 mirnas of 69 insects, 96,925 pirnas of drosophila melanogaster and chilo suppressalis, 2439 lncrna of n ...	2015	26578584
ran involved in the development and reproduction is a potential target for rna-interference-based pest management in nilaparvata lugens.	ran (rangtpase) in insects participates in the 20-hydroxyecdysone signal transduction pathway in which downstream genes, ftz-f1, krüppel-homolog 1 (kr-h1) and vitellogenin, are involved. a putative ran gene (nlran) was cloned from nilaparvata lugens, a destructive phloem-feeding pest of rice. nlran has the typical ran primary structure features that are conserved in insects. nlran showed higher mrna abundance immediately after molting and peaked in newly emerged female adults. among the examined ...	2015	26554926
rnai knockdown of acetyl-coa carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, nilaparvata lugens.	a major challenge in ecology lies in understanding the coexistence of intraguild species, well documented at the organismal level, but not at the molecular level. this study focused on the effects of the antibiotic, jinggangmycin (jgm), a fungicide widely used in asian rice agroecosystems, on reproduction of insects within the planthopper guild, including the brown planthopper (bph) nilaparvata lugens and the white-backed planthopper (wbph) sogatella furcifera, both serious resurgence rice pests ...	2015	26482193
rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant.	most plant viruses that seriously damage agricultural crops are transmitted by insects. however, the mechanisms enabling virus transmission by insect vectors are poorly understood. the brown planthopper (nilaparvata lugens) is one of the most serious rice pests, causing extensive damage to rice plants by sucking the phloem sap and transmitting viruses, including rice ragged stunt virus (rrsv). in this study, we investigated the mechanisms of rrsv transmission from its insect vector to the rice p ...	2015	26073458
roles of nlaktip in the growth and eclosion of the rice brown planthopper, nilaparvata lugens stål, as revealed by rna interference.	akt-interacting protein (aktip) interacts with serine/threonine protein kinase b (pkb)/akt. aktip modulates akt's activity by enhancing the phosphorylation of the regulatory site and plays a crucial role in multiple biological processes. in this study, the full length cdna of nlaktip, a novel aktip gene in the brown planthopper (bph) nilaparvata lugens, was cloned. the reverse transcription quantitive pcr (rt-qpcr) results showed that the nlaktip gene was strongly expressed in gravid female adul ...	2015	26402675
comparing gene expression profiles between bt and non-bt rice in response to brown planthopper infestation.	bt proteins are the most widely used insecticidal proteins in transgenic crops for improving insect resistance. we previously observed longer nymphal developmental duration and lower fecundity in brown planthopper (bph) fed on bt rice line kmd2, although bt insecticidal protein cry1ab could rarely concentrate in this non-target rice pest. in the present study, we performed microarray analysis in an effort to detect bt-independent variation, which might render bt rice more defensive and/or less n ...	2015	26734057
pathways of amino acid degradation in nilaparvata lugens (stål) with special reference to lysine-ketoglutarate reductase/saccharopine dehydrogenase (lkr/sdh).	nilaparvata lugens harbors yeast-like symbionts (ylss). in present paper, a genome-wide analysis found 115 genes from ni. lugens and 90 genes from ylss that were involved in the metabolic degradation of 20 proteinogenic amino acids. these 205 genes encoded for 77 enzymes. accordingly, the degradation pathways for the 20 amino acids were manually constructed. it is postulated that ni. lugens can independently degrade fourteen amino acids (threonine, alanine, glycine, serine, aspartate, asparagine ...	2015	26000452
genome-wide identification of long noncoding rna genes and their potential association with fecundity and virulence in rice brown planthopper, nilaparvata lugens.	the functional repertoire of long noncoding rna (lncrna) has been characterized in several model organisms, demonstrating that lncrna plays important roles in fundamental biological processes. however, they remain largely unidentified in most species. understanding the characteristics and functions of lncrna in insects would be useful for insect resources utilization and sustainable pest control.	2015	26437919
transgenic cry1c or cry2a rice has no adverse impacts on the life-table parameters and population dynamics of the brown planthopper, nilaparvata lugens (hemiptera: delphacidae).	transgenic rice producing the insecticidal protein from bacillus thuringiensis berliner (bt) is protected from damage by lepidopteran insect pests. however, one of the main concerns about bt rice is the potential impact on non-target herbivores. in the present study, the ecological impacts of two bt rice lines, t1c-19 expressing cry1c protein and t2a-1 expressing cry2a protein, on the non-target herbivore brown planthopper (bph), nilaparvata lugens (stål), were evaluated under laboratory and fie ...	2015	25067834
single and fused transgenic bacillus thuringiensis rice alter the species-specific responses of non-target planthoppers to elevated carbon dioxide and temperature.	the approval of transgenic bacillus thuringiensis (bt) rice by china was momentous for biotech crops, although it has yet to be approved for commercial production. non-target pest problems in rice paddies, such as the three ecologically similar species of planthoppers nilaparvata lugens, laodelphax striatellus and sogatella furcifera, could become increasingly serious under global climate change. fused (cry1ab/cry1ac) and single (cry1ab) transgenic bt rice were evaluated for effects on species-s ...	2014	24136625
genomes of the rice pest brown planthopper and its endosymbionts reveal complex complementary contributions for host adaptation.	the brown planthopper, nilaparvata lugens, the most destructive pest of rice, is a typical monophagous herbivore that feeds exclusively on rice sap, which migrates over long distances. outbreaks of it have re-occurred approximately every three years in asia. it has also been used as a model system for ecological studies and for developing effective pest management. to better understand how a monophagous sap-sucking arthropod herbivore has adapted to its exclusive host selection and to provide in ...	2014	25609551
genomic insights into the serine protease gene family and expression profile analysis in the planthopper, nilaparvata lugens.	the brown planthopper (nilaparvata lugens) is one of the most destructive rice plant pests in asia. n. lugens causes extensive damage to rice by sucking rice phloem sap, which results in hopper burn (complete death of the rice plants). despite its importance, little is known about the digestion, development and defense mechanisms of this hemimetabolous insect pest. in this study, we aim to identify the serine protease (sp) and serine protease homolog (sph) genes, which form a large family in euk ...	2014	24952583
transcriptome analysis of fat bodies from two brown planthopper (nilaparvata lugens) populations with different virulence levels in rice.	the brown planthopper (bph), nilaparvata lugens (stål), one of the most serious rice insect pests in asia, can quickly overcome rice resistance by evolving new virulent populations. the insect fat body plays essential roles in the life cycles of insects and in plant-insect interactions. however, whether differences in fat body transcriptomes exist between insect populations with different virulence levels and whether the transcriptomic differences are related to insect virulence remain largely u ...	2014	24533099
diapause induction and termination in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae).	the small brown planthopper, laodelphax striatellus (fallén) enters the photoperiodic induction of diapause as 3rd or 4th instar nymphs. the photoperiodic response curves in this planthopper showed a typical long-day response type with a critical daylength of approximately 11 h at 25 °c, 12 h at 22 and 20 °c and 12.5 h at 18 °c, and diapause induction was almost abrogated at 28 °c. the third stage was the most sensitive stage to photoperiod. the photoperiodic response curve at 20 °c showed a gra ...	2014	25188306
the genome of the nucleopolyhedrosis-causing virus from tipula oleracea sheds new light on the nudiviridae family.	a large double-stranded dna (dsdna) virus that produces occlusion bodies, typical of baculoviruses, has been described to infect crane fly larvae of the genus tipula (diptera, tipulidae). because of a lack of genomic data, this virus has remained unclassified. electron microscopy of an archival virus isolated from tipula oleracea, t. oleracea nudivirus (tonv), showed irregularly shaped occlusion bodies measuring from 2 to 5 μm in length and 2 μm in middiameter, filled with rod-shape virions cont ...	2014	25540386
brown planthopper nudivirus dna integrated in its host genome.	the brown planthopper (bph), nilaparvata lugens (hemiptera:delphacidae), is one of the most destructive insect pests of rice crops in asia. nudivirus-like sequences were identified during the whole-genome sequencing of bph. pcr examination showed that the virus sequences were present in all of the 22 bph populations collected from east, southeast, and south asia. thirty-two of the 33 nudivirus core genes were identified, including 20 homologues of baculovirus core genes. in addition, several gen ...	2014	24574410
transovarial transmission of a plant virus is mediated by vitellogenin of its insect vector.	most plant viruses are transmitted by hemipteroid insects. some viruses can be transmitted from female parent to offspring usually through eggs, but the mechanism of this transovarial transmission remains unclear. rice stripe virus (rsv), a tenuivirus, transmitted mainly by the small brown planthopper (laodelphax striatellus), is also spread to the offspring through the eggs. here, we used the rsv-planthopper system as a model to investigate the mechanism of transovarial transmission and demonst ...	2014	24603905
nonstructural protein ns4 of rice stripe virus plays a critical role in viral spread in the body of vector insects.	rice stripe virus (rsv), a tenuivirus, is transmitted by small brown planthopper (sbph) in a persistent-propagative manner. in this study, sequential infection of rsv in the internal organs of sbph after ingestion of virus indicated that rsv initially infected the midgut epithelium, and then progressed to the visceral muscle tissues, through which rsv spread to the entire alimentary canal. finally, rsv spread into the salivary glands and reproductive system. during viral infection, the nonstruct ...	2014	24523924
constructing the major biosynthesis pathways for amino acids in the brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae), based on the transcriptome data.	nilaparvata lugens is a serious phloem-feeding pest of rice throughout asia. rice phloem sap can meet its nutrition requirement for sugars but not for some essential amino acids such as isoleucine, leucine, methionine, phenylalanine, tryptophan, lysine, arginine and histidine. n. lugens harbours yeast-like symbionts in mycetocytes formed by abdominal fat body cells. removal of the symbionts results in negative physiological effects, suggesting that the symbionts play a pivotal role in the nitrog ...	2014	24330026
phloem-specific expression of the lectin gene from allium sativum confers resistance to the sap-sucker nilaparvata lugens.	rice production is severely hampered by insect pests. garlic lectin gene (asal) holds great promise in conferring protection against chewing (lepidopteran) and sap-sucking (homopteran) insect pests. we have developed transgenic rice lines resistant to sap-sucking brown hopper (nilaparvata lugens) by ectopic expression of asal in their phloem tissues. molecular analyses of t0 lines confirmed stable integration of transgene. t1 lines (np 1-2, 4-3, 11-6 & 17-7) showed active transcription and trans ...	2014	24563293
characterization of the distal-less gene homologue, nldll, in the brown planthopper, nilaparvata lugens (stål).	the brown planthopper, nilaparvata lugens (stål), is a globally devastating insect pest of rice, particularly in eastern asia. distal-less or dll is a highly conserved and well studied transcription factor required for limb formation in invertebrates and vertebrates. we have identified a homologue of this gene, nldll, and demonstrated that it is expressed in all life stages of n. lugens, particularly in adult brachypterous females. when we compared between specific adult tissues it was expressed ...	2014	24321689
seven-membered azabridged neonicotinoids: synthesis, crystal structure, insecticidal assay, and molecular docking studies.	to study the influence of the ring sizes, 37 novel seven-membered azabridged neonicotinoid analogues were synthesized by reactions of nitromethylene analogues, succinaldehyde, and aniline hydrochlorides. most of the title compounds presented higher insecticidal activities than that of imidacloprid (imi), cycloxaprid (cyc), and eight-membered compounds against cowpea aphid (aphis craccivora), armyworm (pseudaletia separata walker), and brown planthopper (nilaparvata lugens), which indicated that ...	2014	25347284
design, synthesis, crystal structures, and insecticidal activities of eight-membered azabridge neonicotinoid analogues.	three series of novel azabridge neonicotinoid analogues were designed and synthesized, which were constructed by starting material a, glutaraldehyde, and primary amine hydrochlorides (aliphatic amines, phenylhydrazines, and anilines). most of the eight-membered azabridge compounds presented higher insecticidal activities than oxabridged compound b against cowpea aphid (aphis craccivora) and brown planthopper (nilaparvata lugens). compared with imidacloprid, some azabridged compounds exhibited ex ...	2014	24364696
infection of rice plants by rice black streaked dwarf virus improves an egg parasitoid, anagrus nilaparvatae (hymenoptera: mymaridae), of rice planthoppers.	the effects of rice plants infected by rice black streaked dwarf virus (rbsdv) on the host preference, duration of immature stages, sex ratio, and adult longevity and parasitic capacity of an egg parasitoid, anagrus nilaparvatae pang et wang, of rice brown planthopper, nilaparvata lugens stål, were evaluated. tests of response to plant volatiles using an olfactometer showed that a. nilaparvatae preferred rice plants harboring rice brown planthopper eggs over plants free of rice brown planthopper ...	2014	25199055
virus-mediated chemical changes in rice plants impact the relationship between non-vector planthopper nilaparvata lugens stål and its egg parasitoid anagrus nilaparvatae pang et wang.	in order to clarify the impacts of southern rice black-streaked dwarf virus (srbsdv) infection on rice plants, rice planthoppers and natural enemies, differences in nutrients and volatile secondary metabolites between infected and healthy rice plants were examined. furthermore, the impacts of virus-mediated changes in plants on the population growth of non-vector brown planthopper (bph), nilaparvata lugens, and the selectivity and parasitic capability of planthopper egg parasitoid anagrus nilapa ...	2014	25141278
a novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host.	rice black-streaked dwarf virus (rbsdv) naturally infects gramineae plants through small brown planthopper (sbph) as a vector. however, rbsdv cannot be transmitted to the sbph offspring through transovarian transmission. wheat plant, an important intermediate host in winter, is essential for the completion of the annual cycle of rbsdv in farm ecosystem. we developed a novel, in vivo, indoor method to preserve rbsdv in sbph using wheat seedling as a bridge host. the temperature range of 23-27°c w ...	2014	25075933
synergism between southern rice black-streaked dwarf virus and rice ragged stunt virus enhances their insect vector acquisition.	southern rice black-streaked dwarf virus (srbsdv), a tentative species in the genus fijivirus, family reoviridae, is a novel rice virus transmitted by the white-backed planthopper (sogatella furcifera). since its discovery in 2001, srbsdv has spread rapidly throughout eastern and southeastern asia and caused large rice losses in china and vietnam. rice ragged stunt virus (rrsv) (genus oryzavirus, family reoviridae) is a common rice virus vectored by the brown planthopper (nilaparvata lugens). rr ...	2014	24915431
unusual characteristics of dicistrovirus-derived small rnas in the small brown planthopper, laodelphax striatellus.	in this study, sequences of small rna (srna) libraries derived from the insect vector laodelphax striatellus were assembled into contigs and used as queries for database searches. a large number of contigs were highly homologous to the genome sequence of an insect dicistrovirus, himetobi p virus (hipv). interestingly, hipv-derived srnas had a wide size distribution, and were relatively abundant throughout the 18-30 nt size range with only a slight peak at 22 nt. hipv srnas had a strong bias towa ...	2014	24323637
identification of himetobi p virus in the small brown planthopper by deep sequencing and assembly of virus-derived small interfering rnas.	profiling and assembly of virus-derived small interfering rnas (sirnas) using next-generation sequencing technologies have been very useful for identification and diagnosis of a number of plant and invertebrate viruses. in this work, we have conducted high-throughput pyrosequencing and bioinformatic analysis of the small brown planthopper (sbph, laodelphax striatellus), and these analyses unexpectedly showed that the himetobi p virus (hipv) was present in our laboratory cultures. hipv was also f ...	2014	24239755
selection of beauveria isolates pathogenic to adults of nilaparvata lugens.	the brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae), is a destructive invasive pest and has become one of the most economically-important rice pests in china. effective control measures are desperately needed. entomopathogenic fungi, such as beauveria bassiana (balsamo-crivelli) vuillemin (hypocreales: clavicipitaceae) and b. brongniartii (saccardo), have shown great potential for the management of some sucking pest species. in this study, to explore alternative strategies fo ...	2014	25373179
[stress effects of imidacloprid on rsv in rice plants].	the rice stripe disease is a viral disease transmitted by small brown planthopper, laodelphax striatellus, which outbroke a few years ago in the yangtze river basin, especially jiangsu region, china. to study the effects of imidacloprid stress on rice stripe virus (rsv) in rice plants, the rice seedlings were treated with imidacloprid 1, 2, 3 and 4 times (b1, b2, b3 and b4), respectively, after artificial inoculation by l. striatellus for 48 h, and the expression levels of relative genes includi ...	2014	25876413
infection route of rice grassy stunt virus, a tenuivirus, in the body of its brown planthopper vector, nilaparvata lugens (hemiptera: delphacidae) after ingestion of virus.	rice grassy stunt virus (rgsv), a tenuivirus, is transmitted by the brown planthopper (bph), nilaparvata lugens (hemiptera, delphacidae), in a persistent-propagative manner. in this study, immunofluorescence microscopy was used to investigate the infection route of rgsv in the internal organs of bph after acquiring the virus by feeding on rgsv-infected rice plants. the sequential infection study revealed that rgsv initially infected the midgut epithelium, then crossed the basal lamina into the m ...	2014	24768845
development of continuous cell culture of brown planthopper to trace the early infection process of oryzaviruses in insect vector cells.	rice ragged stunt virus (rrsv), an oryzavirus in the family reoviridae, is transmitted by the brown planthopper, nilaparvata lugens, in a persistent-propagative manner. here, we established a continuous cell line of brown planthopper to investigate the mechanism underlying the formation of the viroplasm, the putative site for viral replication and assembly, during infection of rrsv in its insect vector cells. within 24 h of viral infection of cultured cells, the viroplasm had formed and containe ...	2014	24478421
southern rice black-streaked dwarf virus alters insect vectors' host orientation preferences to enhance spread and increase rice ragged stunt virus co-infection.	in recent years, southern rice black-streaked dwarf virus (srbsdv), a tentative species in the genus fijivirus (family reoviridae), has spread rapidly and caused serious rice losses in eastern and southeastern asia. with this virus spread, rice ragged stunt virus (rrsv, genus oryzavirus, family reoviridae) became more common in southern china, usually in co-infection with the former. srbsdv and rrsv are transmitted by two different species of planthoppers, white-backed planthopper (wbph, sogatel ...	2014	24047253
influence of rice black streaked dwarf virus on the ecological fitness of non-vector planthopper nilaparvata lugens (hemiptera: delphacidae).	rice black streak dwarf virus (rbsdv) is transmitted by the small brown planthopper (sbph), laodelphax striatellus (fallen). non-vector rice brown planthopper (bph), nilaparvata lugens (stål), shares the same host rice plants with sbph in paddy fields. the changes in nutritional composition of rice plants infected by rbsdv and the ecological fitness of bph feeding on the infected plants were studied under both artificial climate chamber and field conditions. contents of 16 detected amino acids a ...	2014	23956237