Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| electrospray mass spectrometry for characterization of lipid a from enterobacter agglomerans. | negative ion and positive ion electrospray mass spectrometry have been employed to characterize the lipid a mixture produced by hydrolysis of lipopolysaccharides from enterobacter agglomerans, a gram-negative bacterium commonly found in field cotton. neutral monophosphoryl lipid a molecules form stable anions via deprotonation, but adduct formation via cation attachment occurs in low yield. dephosphorylated lipid a molecules, on the other hand, readily form adducts with na+, whereas deprotonatio ... | 1993 | 8431503 |
| lipopolysaccharide-binding proteins of limulus amebocyte lysate. | limulus amebocyte lysate, obtained from horseshoe crab (limulus polyphemus) blood cells, contains a coagulation system which is activated by bacterial lipopolysaccharide (lps). a chromatographic fraction of limulus lysate, containing the endotoxin-sensitive factor(s) which initiates the coagulation cascade, was studied. we utilized a photoreactive, cleavable, radiolabeled derivative of salmonella minnesota lps, lps-(p-azidosalicylamido)-1,3'-dithiopropionamide (lps-asd), to identify lps-binding ... | 1993 | 8432586 |
| domain structure, functional activity, and polymerization of trout complement protein c9. | the 3' region of trout c9 has been resequenced and found to differ from the previously published sequence (stanley and herz, embo j. 6:1951; 1987). in contrast to other sequenced c9 molecules, but in common with the other terminal complement components, trout c9 was found to contain an additional carboxy terminal thrombospondin domain. this domain does not restrict polymerization, as has been previously suggested (stanley and luzio, nature 334:475; 1988), since alternative pathway activation of ... | 1993 | 8449252 |
| comparison of lipids a of several salmonella and escherichia strains by 252cf plasma desorption mass spectrometry. | plasma desorption mass spectrometry has recently been used with success to characterize underivatized lipid a preparations: the major molecular species present give signals indicating their masses, from which probable compositions could be inferred by using the overall composition determined by chemical analyses. in the present study, plasma desorption mass spectrometry was used to compare structures in lipid a preparations isolated from several smooth and rough strains of escherichia and salmon ... | 1993 | 8491718 |
| inhibition of endotoxin-induced interleukin 8 release by teicoplanin in human whole blood. | antibiotic-induced endotoxin (lipopolysaccharide; lps) release may precipitate septic shock. in the present study the effect of teicoplanin, which has been reported to neutralize lps in experimental models, on lps neutralization was investigated in human whole blood samples. levels of interleukin 8, a preinflammatory cytokine which was stimulated by salmonella minnesota r595 lps (12.6 micrograms/ml), were monitored over time. interleukin 8 concentrations increased over time up to 24 h. when lps ... | 1993 | 8187790 |
| [the use of monoclonal antibodies to lipid a for the correction of the hemodynamic disorders in endotoxemia]. | endotoxemia induced by gram-negative bacteria leads to endotoxic shock pathogenetically stemming from the integral component of the bacterial wall--lipid a. the study made to define the ability of lipid a monoclonal antibodies to correct hemodynamic disturbances due to endotoxemia in dog experiments showed the efficacy of the antibodies administration. relps isolated from salmonella minnesota was used as an antigen. administration of the complex monoclonal antibodies-endotoxin caused no hemodyna ... | 1993 | 8020702 |
| polymorphism of crystals of salmonella minnesota re and ra lipopolysaccharides. | salmonella minnesota re and ra lipopolysaccharides (lpss) formed three-dimensional crystals when they were precipitated by the addition of 2 volumes of 95% ethanol containing 375 mm mgcl2 and incubated in 70% ethanol containing 250 mm mgcl2 at 4 c. besides typical shapes of crystals, hexagonal plates and solid columns, which were already reported (j. bacteriol. 172: 1516-1528 (1990)), the lpss thus treated formed crystals possessing various shapes such as square or rectangular plate, lozenge pla ... | 1993 | 8231969 |
| endotoxin stimulates drinking in rats without changing dehydrational signals controlling thirst. | intravenous injections of endotoxins from escherichia coli or salmonella minnesota stimulate drinking and reduce urinary excretion of water and solutes in rats. e. coli endotoxin (0.15 or 0.45 mg/kg i.v.) stimulated drinking without increasing plasma osmolality or sodium concentration, hematocrit, blood hemoglobin, or plasma protein concentration and without decreasing arterial pressure. similarly, a dipsogenic dose of s. minnesota endotoxin (0.25 mg/kg i.v.) did not reduce arterial or venous pr ... | 1993 | 8238605 |
| stimulation of drinking by bacterial endotoxins in the rat. | we investigated the effects of endotoxins on water balance, rectal temperature, and food intake in male long-evans rats with femoral venous catheters. extracts of escherichia coli or salmonella minnesota, in doses ranging from 125 to 500 micrograms/kg iv, stimulated drinking and reduced urinary water loss for several hours. the net gain of 5 ml water 2 h after the lowest dose of e. coli endotoxin was sufficient to reduce plasma osmolality and sodium concentration 2 to 3%. drinking occurred durin ... | 1993 | 8248364 |
| structural characterization of gangliosides from resting and endotoxin-stimulated murine b lymphocytes. | b lymphocytes from cba/j mice were stimulated in splenocyte cultures for 72 h with various endotoxins. bisphosphoryl lipid a from escherichia coli had the highest stimulatory effect followed by lps of citrobacter freundii and salmonella minnesota as measured by [3h]thymidine uptake. gangliosides of stimulated b cells (metabolically labeled with d-[1-14c]galactose and d-[1-14c]glucosamine) and unlabeled gangliosides from resting b cells (prepared from spleens without stimulus) were analyzed by hi ... | 1993 | 8251488 |
| influence of the supramolecular structure of free lipid a on its biological activity. | the three-dimensional supramolecular structures and the states of order of the acyl chains of lipid a from different gram-negative species were investigated at 40 degrees c, high water content (80-90%), and different [lipid a]/[mg2+] molar ratios using synchrotron radiation x-ray diffraction and fourier-transform infrared spectroscopy. measurements were made on free lipid a from salmonella minnesota r595, mono- and bi-phosphoryl, as well as those from the non-enterobacterial strains rhodobacter ... | 1993 | 8269946 |
| specificity of rabbit antisera against the rough lipopolysaccharide of salmonella minnesota strain r7 (chemotype rd1p-). | rabbit polyclonal antibodies against the lipopolysaccharide (lps) of the rd1p- mutant strain r7 of salmonella minnesota were serologically characterized using r7 lps, dephosphorylated lps, deacylated lps, deacylated, dephosphorylated and reduced lps, and synthetic partial structures. the latter comprised partial structures of the core region of rd1p- lps bound to the beta 1-->6-linked glucosamine dissacharide with two amide-linked 3-hydroxytetradecanoic acid residues or artificial glycoconjugate ... | 1993 | 8275057 |
| sugar-binding sites with specificity for glucosamine in the guinea pig middle ear mucosa. | glucosamine-binding sites were detected in lowicryl k4m-embedded guinea pig middle ear mucosa by electron microscopy, using glucosaminyl bovine serum albumin. incubation of ultrathin tissue sections with gold-labeled glucosaminyl bovine serum albumin (glcn/bsa/gold) resulted in binding mainly on cilia, microvilli, rough endoplasmic reticulum and nuclei. the sugar binding was not inhibited after ultrathin sections had been digested with trypsin or neuraminidase. various carbohydrates and glycocon ... | 1993 | 8286107 |
| specificity of rabbit antisera against the rough lipopolysaccharide of salmonella minnesota r4 (chemotype rd2p-) | rabbit polyclonal antibodies against the rough mutant lipopolysaccharide (lps) of salmonella minnesota r4 (chemotype rd2p-) were serologically characterized by using r4 lps, deacylated lps, dephosphorylated lps, and synthetic partial structures, including compounds comprising the core region of rd2p- lps bound to the beta 1-->6-linked glucosamine disaccharide with two amide-linked 3-hydroxytetradecanoic acid residues or coupled to bovine serum albumin. by using a passive hemolysis assay and an e ... | 1993 | 8335352 |
| the outer membranes of brucella spp. are not barriers to hydrophobic permeants. | the patterns of susceptibility to hydrophobic and hydrophilic drugs and the uptake of the fluorescent probe n-phenyl-naphthylamine in brucella spp., haemophilus influenzae, escherichia coli, and deep rough salmonella minnesota mutants were compared. the results show that the outer membranes of smooth and naturally rough brucella spp. do not represent barriers to hydrophobic permeants and that this absence of a barrier relates at least in part to the properties of brucella lipopolysaccharide. | 1993 | 8349567 |
| serum-independent binding of lipopolysaccharide to human monocytes is trypsin sensitive and does not involve cd14. | the nature of the binding sites for lipopolysaccharide (lps) on human monocytes was investigated using fluorescein isothiocyanate (fitc)-labelled lps from salmonella minnesota r595 (relps). in the absence of serum, relps bound to monocytes and this interaction was trypsin sensitive. a concentration of 0.1 mg/ml resulted in a 90% loss of lps binding, while low concentrations increased this binding. trypsin-treated monocytes recovered fitc-relps binding after 20 hr culture, which was abrogated in ... | 1993 | 7503949 |
| lipopolysaccharide binding protein and cd14 interaction induces tumor necrosis factor-alpha generation and neutrophil sequestration in lungs after intratracheal endotoxin. | it has been proposed that lipopolysaccharide (lps) bound to the 60-kd lps binding protein (lbp) forms an lps/lbp complex that, in turn, binds to the cd14 receptor on monocytes/macrophages and stimulates the release of cytokines. we examined the role of lbp and cd14 in tumor necrosis factor-alpha (tnf-alpha) production and neutrophil (polymorphonuclear leukocyte [pmn]) sequestration in lungs induced by intratracheal instillation of lps using rabbit lungs perfused at constant flow with lactated ri ... | 1993 | 7685250 |
| traces of bacterial lipopolysaccharide suppress ifn-gamma-induced nitric oxide synthase gene expression in primary mouse macrophages. | a nitric oxide synthase (inos) inducible by cytokines and microbial products contributes to the cytotoxic and antimicrobial activity of mouse macrophages. bacterial lps interacts synergistically with ifn-gamma to induce inos when both stimuli are added together. in contrast, we show here that pre-exposure of peritoneal macrophages to low concentrations of lps suppresses the induction of inos when ifn-gamma is added subsequently. suppression required pretreatment with lps for at least 8 h and was ... | 1993 | 7686937 |
| comparison of x-ray powder-diffraction data of various bacterial lipopolysaccharide structures with theoretical model conformations. | x-ray powder-diffraction experiments have been performed on dry samples of lipid a and various rough-mutant lipopolysaccharides (lps) of salmonella minnesota, salmonella typhimurium and escherichia coli. the diffraction patterns obtained indicated exclusively lamellar, bilayered arrangements in all samples. the periodicities were found to be in the range 4.5 nm for lipid a to 8.8 nm for ra-lps. upon treatment with water-saturated air, swelling of the lamellar structures was achieved, as indicate ... | 1993 | 8223620 |
| lipopolysaccharide (lps) binding to 73-kda and 38-kda surface proteins on lymphoreticular cells: preferential inhibition of lps binding to the former by rhodopseudomonas sphaeroides lipid a. | using a photoactivable, radioiodinated lipopolysaccharide probe, [125i]asd-lps (derivatized from purified e. coli 0111:b4 s-lps), we earlier reported the presence of a 73-kda (p73) predominant lps-binding protein on mouse lymphocytes and macrophages with specificity for the lipid a region of lps. both re-lps from salmonella minnesota and purified lipid a will inhibit the binding of lps to the p73 lps receptor. in the studies reported here, we have found that non-toxic diphosphoryl lipid a purifi ... | 1993 | 7690343 |
| anti-endotoxin monoclonal antibodies protect by enhancing bacterial and endotoxin clearance. | in this study, we sought to determine the mechanism(s) by which a type-specific anti-lipopolysaccharide monoclonal antibody (an igg directed against the o-antigen polysaccharide region of salmonella minnesota lipopolysaccharide) and its f(ab')2 fragments protect during gram-negative bacterial peritonitis and endotoxemia in mice. during peritoneal infection, (1) igg significantly decreased mortality, bacteremia, and endotoxemia at all time points compared with saline solution pretreatment and (2) ... | 1993 | 7679267 |
| human monoclonal antibody ha-1a binds to endotoxin via an epitope in the lipid a domain of lipopolysaccharide. | ha-1a, a human igm mab, has been shown to significantly reduce mortality in septic patients with gram-negative bacteremia, especially those with septic shock, in a controlled clinical trial. to confirm the reported specificity of this antibody for the lipid a domain of endotoxin, several assay systems were developed. these assay systems included an elisa, which measured the binding of ha-1a to lipid a adsorbed to a solid phase; a rate nephelometry assay, which measured the ability of ha-1a to bi ... | 1993 | 7683319 |
| effects of two magainin peptides on eicosanoid release from rat peritoneal macrophages. | magainins are novel polycationic peptides with broad-spectrum antimicrobial activity, including activity against gram-negative bacteria. gram-negative bacteremia can elicit endotoxic shock that is associated with the increased formation of eicosanoids. inhibition of eicosanoid synthesis has been shown to improve the outcome of experimental endotoxic shock. the aim of the present study was to test the in vitro effects of two magainin peptides, msi-97 (m1) and msi-98 (m2), on eicosanoid synthesis ... | 1993 | 8460910 |
| abrogation of suppression of delayed hypersensitivity induced by candida albicans-derived mannan by treatment with monophosphoryl lipid a. | monophosphoryl lipid a (mla), derived either from salmonella minnesota or salmonella typhimurium, was tested for its ability to alter candida albicans mannan (man)-specific suppression. since we showed previously that naive mice injected intravenously (i.v.) with man developed suppressor t cells capable of down-regulating delayed-type hypersensitivity when transferred to immunized recipients, mla was tested for its ability to influence suppressor activity in the donors of suppressor cells. t-lym ... | 1993 | 8478102 |
| research note: differential chemotaxis of physarum polycephalum to salmonella gallinarum and salmonella pullorum. | the chemotactic and chemotropic responses of the plasmodial stage of the slime mold physarum polycephalum were used to distinguish salmonella gallinarum and salmonella pullorum from 10 salmonella serovars that are commonly isolated from domestic poultry. utilizing an in vitro plasmodium agar plate assay method, p. polycephalum was attracted to s. gallinarum and s. pullorum, but the organism was repelled by salmonella derby, salmonella dublin, salmonella enteritidis, salmonella heidelberg, salmon ... | 1993 | 8479960 |
| cross-binding activity and protective capacity of monoclonal antibodies to lipid a. | six hybridoma clones (1m, 4m, 9m, 11m, 18m and 31g), secreting monoclonal antibodies (mabs) against lipid a were obtained after fusion between cells of mouse myeloma line x63-ag8.653 and spleen cells from balb/c mice immunized with acid treated salmonella minnesota bacteria coated with additional free lipid a. the specificity and cross-binding activity of the mabs were characterized in elisa by using synthetic lipid a analogs as well as different lipid a and lipopolysaccharides (lps) extracted f ... | 1993 | 8406552 |
| preparation and binding specificity of a monoclonal antibody recognizing 3-deoxy-d-manno-2-octulosonic acid (kdo) in lipopolysaccharides of re chemotype. | a mouse monoclonal antibody (mab e1) was raised against the lipopolysaccharide (lps) of the re mutant r595 of salmonella minnesota. this igg3 antibody (mab e1), unstable at low ph and low ionic strength, was purified by chromatography on qae sepharose a50. the binding specificity of mab e1 was characterized by direct and inhibition enzyme immunoassays, using natural lpss from different strains and chemotypes, and synthetic analogs of lps substructure of the 3-deoxy-d-manno-2-octulosonic acid (kd ... | 1992 | 1294455 |
| activation of human monocyte chemiluminescence response by acylpoly(1,3)galactosides derived from klebsiella pneumoniae. | the stimulating activity of several preparations isolated from a membrane proteoglycan of a nonencapsulated smooth strain of klebsiella pneumoniae (kp-mpg) on the oxidative burst of human blood monocytes was assessed by luminol-enhanced chemiluminescence (cl). five kp derivatives were studied: a 34-kd acylpoly(1,3)galactoside (apg), obtained by drastic alkaline hydrolysis and purified by chromatography; an apg preparation subjected to acid hydrolysis that removed the core part and all fatty acid ... | 1992 | 1431564 |
| early endotoxin tolerance in suckling rats. | early endotoxin tolerance has not been well investigated in the newborn. this study demonstrated that a sublethal dose of salmonella enteritidis lipopolysaccharide (s. ent-lps) or rough mutant salmonella minnesota lps (re-lps) induced early endotoxin tolerance in suckling rats, showing blunted hypoglycemia and decreased mortality. the mortality of endotoxic shock was lower in s. ent-lps pretreated group than re-lps pretreated group. a sublethal dose of s. ent-lps caused lactacidemia but re-lps d ... | 1992 | 1604049 |
| adhesion molecules involved in the interaction of lgl/nk cells and human endothelial cells stimulated with salmonella bacteria. | previously we showed that pretreatment of lgl/nk or huve cells with salmonella bacteria augments the adhesion of lgl/nk cells to endothelium. here we analyse the roles of huvec adhesion molecules vcam-1, icam-1 and e-selectin, and the counter-receptors vla-4, lfa-1 and slex in the increase of lgl/nk adhesion to huvec, stimulated with salmonella minnesota mr595 bacteria, lps or tnf-alpha. on salmonella-stimulated huvec, vcam-1 and icam-1 were the major binding structures involved, and their effec ... | 1992 | 1279781 |
| binding of a bacterial acylpoly(1,3)galactoside to human blood leucocytes. | the binding of a 34-kda (mol. wt.) acylpoly(1,3)galactoside (apg) extracted from a membrane proteoglycan of klebsiella pneumoniae to human blood leucocytes was investigated. apg is made of a long poly(1,3)galactose chain, a core-like region and a lipid moiety which comprises two glucosamine residues bound to a phosphate group and two beta oh myristic acids. fluoresceinated apg was shown to bind preferentially to monocytes and to a lesser extent to polymorphonuclear neutrophils, as determined by ... | 1992 | 1615280 |
| lipopolysaccharide binding protein enhances the responsiveness of alveolar macrophages to bacterial lipopolysaccharide. implications for cytokine production in normal and injured lungs. | a plasma lipopolysaccharide (lps)-binding protein (lbp) has been shown to regulate the response of rabbit peritoneal macrophages and human blood monocytes to endotoxin (lps). we investigated whether lbp is present in lung fluids and the effects of lbp on the response of lung macrophages to lps. immunoreactive lbp was detectable in the lavage fluids of patients with the adult respiratory distress syndrome by immunoprecipitation followed by western blotting, and also by specific immunoassay. in ra ... | 1992 | 1281827 |
| [lipopolysaccharide binding protein enhances intratracheally administrated lipopolysaccharide-induced acute lung inflammation via a cd14 receptor]. | we examined the role of lipopolysaccharide binding protein (lbp) in the airspace and the cd14 receptor on alveolar macrophages in tnf alpha production and neutrophil (pmn) sequestration in lungs induced by intratracheal injection of lipopolysaccharide (lps). lps alone (salmonella minnesota wild-type; 20 ng) or lps + lbp complex [lps (20 ng) + rabbit lbp (500 ng); preincubated for 30 min at 37 degrees c] was injected intratracheally into isolated rabbit lungs perfused with lactate-ringer-albumin ... | 1992 | 1284855 |
| immunosuppressive effects induced by the polysaccharide moiety of some bacterial lipopolysaccharides. | the immunomodulatory properties of several lipopolysaccharides (lps) derived from clinical isolates of pseudomonas aeruginosa, branhamella catarrhalis, and bordetella pertussis were evaluated for their capacity to influence the magnitude of the antibody response to type iii pneumococcal polysaccharide (sss-iii), which is known to be regulated by suppressor and amplifier t cells (ts and ta, respectively). the administration of lps, two days after immunization resulted in a significant increase in ... | 1992 | 1286878 |
| monocyte cytokine secretion induced by chemically-defined derivatives of klebsiella pneumoniae. | the capacity of a k. pneumoniae membrane proteoglycan (kp-mpg) and four of its chemically defined derivatives to activate human monocytes was studied by measuring immunoreactive il-1 beta, il-6 and tumour necrosis factor-alpha (tnf-alpha) in culture supernatants. monocyte culture supernatants were also tested for their comitogenic activity on concanavalin a-stimulated thymocytes and for their cytotoxic activity on the mouse fibroblastic l929 cell line. the four kp-mpg derivatives were: (i) an ac ... | 1992 | 1628418 |
| generation of one set of monoclonal antibodies specific for b-pathway ganglio-series gangliosides. | we established six murine monoclonal antibodies (mabs) specific for b-pathway ganglio-series gangliosides by immunizing c3h/hen mice with these purified gangliosides adsorbed to salmonella minnesota mutant r595. the binding specificities of these mabs were determined by an enzyme-linked immunosorbent assay and immunostaining on thin-layer chromatogram. these six mabs, designated ggb19, gmr2, gmr7, ggr12, gmr5, and ggr13 reacted strongly with the gangliosides gd3, o-ac-gd3, gd2, gd1b, gt1b, and g ... | 1992 | 1371229 |
| a synthetic glycoconjugate representing the genus-specific epitope of chlamydial lipopolysaccharide exhibits the same specificity as its natural counterpart. | the tetrasaccharide 3-deoxy-alpha-d-manno-2-octulosonic acid (alpha-kdo) (2----8)-alpha-kdo(2----4)-alpha-kdo(2----6)-beta glcnac, a partial structure of chlamydial lipopolysaccharide (lps) representing a genus-specific epitope, was synthesized and covalently linked to bovine serum albumin, resulting in an artificial glycoconjugate antigen. mice were immunized with the glycoconjugate to prepare chlamydia-specific monoclonal antibodies. they were selected with chlamydia-specific lps antigens and ... | 1992 | 1372290 |
| purification and characterization of lps-free porins isolated from salmonella minnesota. | porins of salmonella minnesota, r595, were purified by anion exchange chromatography and subsequently isolated in their monomeric form by chromatofocusing. two forms of porin could be isolated, both with an apparent molecular mass of 37,000, but of differing isoelectric points (pi 4.6 versus pi of 4.9). porins with pi 4.9 did not contain any detectable lps, but porins with pi 4.6 were found to contain trace amounts of lps (1.3 x 10(-4) micrograms lps/1 microgram porin) as measured using a highly ... | 1992 | 1372873 |
| a monoclonal antibody (st-1) directed to the native heparin chain. | a mouse monoclonal antibody, st-1, was raised against heparin complexed to salmonella minnesota. characterization of this antibody showed that it recognizes an epitope in the intact molecule of heparin that is present regardless of its source or anticoagulant activity. st-1 is the first monoclonal antibody specific for the intact unmodified molecule of heparin to be described. 3h-labeled heparin in solution was immunoprecipitated by st-1, and the formation of the 3h-labeled immunocomplex was sel ... | 1992 | 1377883 |
| reactivity of monoclonal antibody e5 with endotoxin. i. binding to lipid a and rough lipopolysaccharides. | the murine igm monoclonal antibody (mab) e5 was produced by a hybridoma derived from spleen cells of a mouse immunized with the j5 rough mutant of escherichia coli o111:b4. in a multicenter randomized placebo-controlled clinical trial, e5 has been shown to reduce significantly the mortality and morbidity of patients with gram-negative sepsis. the characteristics of e5 binding to endotoxin were studied in vitro. we report here the results of binding to an extensive panel of rough lipopolysacchari ... | 1992 | 1394865 |
| both the polysaccharide and lipid a parts of endotoxins are needed for the inhibitory effects of endotoxins on cellular ldl uptake. | we previously reported that endotoxins inhibit low-density lipoprotein (ldl) uptake and degradation in hep g2 cells. in the present study, we tried to elucidate which part(s) of the endotoxin molecules contribute(s) to the inhibitory effect of endotoxins on ldl uptake and degradation. the results show that the endotoxin isolated from salmonella minnesota re595 (re mutant), which lacks polysaccharide, had no effect on the uptake and degradation of 125i-labelled ldl in hep g2 cells. this mutant fo ... | 1992 | 1411250 |
| [the adhesive properties of s and r mutants of salmonella minnesota during batch cultivation]. | the study of the adhesive, surface-electrical and hydrophobic properties of s. minnesota s- and r-mutants in the process batch cultivation has revealed that the maximum values of the above parameters are characteristic of the stationary phase of culture growth and their minimum values, of the end of the lag phase. electrophoretic mobility and adhesiveness increase from s- to re-chemotype. the surface of salmonella s-form is more hydrophilic, than in r-mutants which are indistinguishable in the d ... | 1992 | 1414109 |
| 252cf plasma-desorption mass spectrometry of lipid a from enterobacter agglomerans. | endotoxins from gram-negative bacteria are believed to be causative agents of byssinosis, an occupational pulmonary disease associated with exposure to cotton dust in textile mills. lipid a preparations from enterobacter agglomerans, a gram-negative bacterium commonly found in cotton and cotton dust, have been analyzed using plasma-desorption mass spectrometry. results indicate the existence of at least two lipid a types which differ only by the presence of an additional oxygen atom whose positi ... | 1992 | 1421514 |
| studies on phospholipids of different mutants of salmonella minnesota. | lipid composition of the cells of smooth (s form) and core-defective rough mutants (ra, rb & re) of salmonella minnesota has been studied. the readily-extractable lipids (rels), acid-extractable lipids, and polar and nonpolar phospholipids have been analysed. fatty acid composition of the different fractions containing phospholipids and other neutral lipids have been determined by glc and gc-ms techniques. phosphatidyl glycerol (pg), phosphatidylethanolamine (pe) and diphosphatidyl glycerol (dpg ... | 1992 | 1427963 |
| mobilization of iron and iron-related proteins in rat spleen after intravenous injection of lipopolysaccharides (lps). | we used lipopolysaccharide (lps) to provoke immune responses and observed the changes in the localization of iron and iron-related proteins, such as transferrin receptor, ferritin and hemosiderin in the rat spleen. after intravenous injection of 250 micrograms lps (salmonella minnesota r595), spleen weight and serum igm levels increased, cells incorporating 5-bromo-2'-deoxyuridine (brdu), and transferrin receptor positive cells increased in the peripheral portion of the periarterial lymphoid she ... | 1992 | 1448384 |
| analysis of the binding of polymyxin b to endotoxic lipid a and core glycolipid using a fluorescent displacement probe. | dansylcadaverine, a cationic fluorescent probe binds to bacterial lipopolysaccharide and lipid a, and is displaced competitively by other compounds which possess affinity toward endotoxins. the binding parameters of dansylcadaverine for lipid a were determined by scatchard analysis to be two apparently equivalent sites with apparent dissociation constants (kd) ranging between 16 microm to 26 microm, while that obtained for core glycolipid from salmonella minnesota re595 yielded a kd of 22 microm ... | 1992 | 1450208 |
| phase diagram of deep rough mutant lipopolysaccharide from salmonella minnesota r595. | the structural polymorphism of deep rough mutant lipopolysaccharide--in many biological systems the most active endotoxin--from salmonella minnesota strain r595 was investigated as function of temperature, water content, and mg2+ concentration. differential scanning calorimetry was used to determine the amount of bound water and the enthalpy change at the beta<==>alpha gel to liquid crystalline acyl chain melting. the onset, midtemperature tc, and completion of the beta<==>alpha phase transition ... | 1992 | 1486008 |
| the salmonella typhimurium virulence plasmid complement resistance gene rck is homologous to a family of virulence-related outer membrane protein genes, including pagc and ail. | a fragment of the salmonella typhimurium virulence plasmid containing the rck locus, when cloned in the recombinant cosmid pade016, was shown previously to confer high-level complement resistance on both rough and smooth escherichia coli, salmonella minnesota, and s. typhimurium and was associated with the production of an outer membrane protein. we determined the nucleotide sequence of the fragment containing the rck locus. mutations in the two major open reading frames confirmed that the compl ... | 1992 | 1729227 |
| protective and pathological roles of nitric oxide in endotoxin shock. | the aim was to investigate the effects of ng-monomethyl-l-arginine (l-nmma), an inhibitor of both the constitutive (ca2+ dependent) and inducible (ca2+ independent) nitric oxide (no) synthases, or of pretreatment with the glucocorticoid dexamethasone, an inhibitor of the induction of the ca2+ independent no synthase, on lipopolysaccharide induced shock in the anaesthetised rabbit. | 1992 | 1516112 |
| lymphoproliferative responses in diet-restricted and aging sprague-dawley rats. | immunological effects of aging and dietary restriction (i.e., chronic underfeeding without malnutrition) were investigated in male cfy sprague-dawley rats. from weaning, diet-restricted animals were given the amount of food that maintained their body weights at approximately 50% of age-matched ad libitum controls. cells from the spleen, peyer's patches, brachial/axillary lymph nodes, and mesenteric nodes of 12- and 20-month-old rats were tested in vitro against the t-cell mitogens phytohaemagglu ... | 1992 | 1521594 |
| teicoplanin reduces in-vitro reactivity and murine lethality of salmonella minnesota r595 lipopolysaccharide. | three different tests were performed to investigate the effect of teicoplanin on lipopolysaccharide (lps). after incubation for 3 h with teicoplanin, lps from salmonella minnesota r595 showed reduced reactivity in the metachromatic dimethyl-methylene blue assay and the limulus amoebocyte lysate test. in addition, galactosamine-sensitized mice had an increased survival rate, from 29% to 72%, when teicoplanin was pre-incubated for 3 h with the lps to be injected intraperitoneally. the results sugg ... | 1992 | 1535068 |
| structural features that influence the ability of lipid a and its analogs to abolish expression of suppressor t cell activity. | lipid a preparations derived from the lipopolysaccharides of several gram-negative bacteria, as well as chemically defined synthetic lipid a's and their analogs (both glucosamine mono- and disaccharides), were used to establish the chemical structures required for (i) abolishing the expression of suppressor t cell (ts) function and (ii) inducing polyclonal activation of b cells. salmonella minnesota r595 lipid a (diphosphoryl lipid a) possesses both of these activities. decreasing the number of ... | 1992 | 1535339 |
| generation of murine monoclonal antibodies specific for n-glycolylneuraminic acid-containing gangliosides. | we generated two murine monoclonal antibodies (mabs) specific for mono- and disialylgangliosides having n-glycolylneuraminic acid (neugc) as their sialic acid moiety, respectively, by immunizing c3h/hen mice with these purified gangliosides adsorbed to salmonella minnesota followed by fusion with mouse myeloma cells. by use of a wide variety of glycolipids, including neugc-containing gangliosides, the precise structures recognized by these two antibodies were elucidated through enzyme-linked imm ... | 1992 | 1567198 |
| use of low toxicity adjuvants and killed virus to induce protective immunity against the friend murine leukaemia retrovirus-induced disease. | low toxic and synthetic adjuvants were investigated in the induction of protective immunity against friend murine retrovirus-induced erythroleukaemia by immunization with inactivated friend murine leukaemia helper virus (f-mulv). 6-o-(2-tetradecyl-hexadecanoyl)-n-acetylmuramyl-l-alanyl-d-isoglutamine (b30-mdp) showed a significant enhancement of the protective immunity against friend virus-induced erythroleukaemia not only in h-2a/b mice known to make good immune responses to f-mulv envelope, bu ... | 1992 | 1574922 |
| monophosphoryl lipid a attenuates the effects of endotoxic shock in pigs. | monophosphoryl lipid a (mpl) is a nontoxic lipid a derivative that maintains many of the beneficial immunomodulatory activities of the parent lipopolysaccharide molecule, including the induction of tolerance to endotoxin. the hemodynamic effects of salmonella minnesota mpl (300 mg/kg) and s. minnesota lipopolysaccharide (300 micrograms/kg) were compared in 20 minipigs. decreases in cardiac output and arterial pressure and increases in pulmonary artery pressure and lactic acidosis were significan ... | 1992 | 1583383 |
| antibody responses to ganglio-series gangliosides in different strains of inbred mice. | we studied antibody responses after immunization with ganglio-series gangliosides against 10 strains of inbred mice, including balb/c, c57bl/6, a/j, c3h/hen, c3h/hej, cba/n, akr/n, nzb/n, dba/2 and nu/nu balb/c. twelve gangliosides having neuac as their sialic acid moiety (gm4, gm3, gm2, gm1, gd3, o-ac-gd3, gd2, gd1a, gd1b, gt1a, gt1b and gq1b), four gangliosides having neugc (gm3, gm2, gm1 and gd3) and four asialo-gangliosides (ga4, ga3, ga2 and ga1) were injected intravenously adsorbed to salm ... | 1992 | 1584231 |
| lipopolysaccharide induces egr-1 mrna and protein in murine peritoneal macrophages. | bacterial lps has diverse effects on the function of immune cells, in general, and macrophages, in particular. the intracellular molecular events that mediate the effects of lps are unclear. we undertook a series of studies in thioglycollate-elicited murine peritoneal macrophages to evaluate the effect of lps on expression of egr-1, a member of the immediate early response gene family. egr-1 may function as an intranuclear "third messenger" because it is rapidly induced in a variety of cell type ... | 1992 | 1401930 |
| induction of antitumor activities in spleen cells from mice and rats activated with lipopolysaccharide immobilized on beads. | lipopolysaccharides (lps) were coupled to polystyrene beads in order to apply the lps without toxicity. the antitumor activity of the lps-immobilizing beads was studied in experiments in vitro and in vivo. in vitro studies showed that spleen cells from c3h/hen mice stimulated by beads immobilizing lps from escherichia coli produced cytolytic activity as strong as that of lymphokine-activated killer (lak) cells. spleen cells from sprague-dawley rats stimulated by beads immobilizing lps from salmo ... | 1992 | 1611621 |
| high-performance liquid chromatographic method to resolve and determine lipopolysaccharide sub-groups of escherichia coli endotoxin in isolated perfused rat liver perfusate. | a high-performance liquid chromatographic method was developed for resolving heterogeneous preparations of fluorescently labelled endotoxin derived from escherichia coli (serotype 0111:b4) into separate lipopolysaccharide sub-groups. the endotoxin was chromatographed on an analytical gel permeation column using a mobile phase of acetonitrile (20%, v/v) and 100 mm phosphate buffer (ph 7.75). four fluorescent peaks were resolved, representing sub-groups of markedly different molecular sizes. three ... | 1992 | 1618951 |
| effect of glycerol monolaurate on bacterial growth and toxin production. | glycerol monolaurate (gml) is a naturally occurring surfactant that has potential use as an additive to tampons and wound dressings to reduce the incidence of certain bacterial toxin-mediated illnesses. in vitro studies were undertaken to evaluate the effect of gml on the growth of and toxin production by potentially pathogenic bacteria. gml inhibited the growth of clinical isolates of group a, b, f, and g streptococci at concentrations of 10 to 20 micrograms/ml. exotoxin production, including t ... | 1992 | 1622174 |
| generation of one set of monoclonal antibodies specific for a-pathway ganglio-series gangliosides. | we established five murine monoclonal antibodies (mabs) specific for a-pathway ganglio-series gangliosides by immunizing c3h/hen mice with these purified gangliosides adsorbed to salmonella minnesota, followed by fusion with mouse myeloma cells. the binding specificities of these mabs were determined by enzyme-linked immunosorbent assay and immunostaining on thin-layer chromatogram. these five mabs, designated gmr6, gmb28, gmb16, gmr17, and gmr11 reacted strongly with the gangliosides gm3, gm2, ... | 1992 | 1627599 |
| mapping of the binding specificity for five monoclonal antibodies recognizing 3-deoxy-d-manno-octulosonic acid in bacterial lipopolysaccharides. | mouse mab were produced against the deep rough strains salmonella minnesota r 595, salmonella typhimurium sl 1102, and escherichia coli d21f2 and screened by enzyme immunoassay against lps of several chemotypes. five antibodies were selected for their ability to bind to chemotype deep rough (re) lps which has two 3-deoxy-d-manno-octulosonic acid (kdo) residues in its nonreducing end. structurally verified oligosaccharides isolated from rough lps and synthetic analogues of kdo were used in an enz ... | 1991 | 2033255 |
| binding profiles of anticardiolipin antibodies in sera from patients with sle and infectious diseases. | inhibition experiments were performed to study the specificity of igg-class antibody, binding to cardiolipin immobilized onto a polystyrene surface, in sera from patients with systemic lupus erythematosus (sle) or infection. six different phospholipids (three anionic: cardiolipin, phosphatidylserine and phosphatidic acid, and three neutral: phosphatidylcholine, phosphatidylethanolamine and platelet activating factor), lipopolysaccharide from salmonella minnesota (relps), strain re595 and lipotei ... | 1991 | 1797029 |
| structural studies of the core region of the lipopolysaccharide from salmonella minnesota strain r7 (rough mutant chemotype rd1). | 1991 | 1804536 | |
| antibodies to core lipid fractions of endotoxin in children with rheumatic disease. | the titer of igg and igm antibodies to two fragments of endotoxin derived from salmonella minnesota r 595, lipid a width a 2-keto-3-deoxyoctanate oliosaccharide (kdo lipid a), and monophosphoryl lipid a (mono-p lipid), were measured in normal children, children with cystic fibrosis, and children with sle and all forms of chronic juvenile arthritis. elevated titers of igm anti mono-p lipid a were found in all of the rheumatic diseases, but not in cystic fibrosis. the elevated igm titers were not ... | 1991 | 1845405 |
| bacterial lipopolysaccharide stimulates protein tyrosine phosphorylation in macrophages. | lipopolysaccharide (lps), a membrane component of gram-negative bacteria, stimulates immune responses by activating macrophages, b lymphocytes, and other cells of the immune system. the mechanisms by which lps activates these cells are poorly characterized. since protein tyrosine phosphorylation appears to be a major intracellular signaling event that mediates cellular responses, we examined whether lps alters tyrosine phosphorylation in macrophages. we found that escherichia coli k235 lps incre ... | 1991 | 1709735 |
| effect of salmonella bacteria on the interaction of human nk cells with endothelial cells. | leukocyte-endothelial cell adhesion and its regulation are essential and complex initial aspects of lymphocyte migration. various factors (il-1, tnf-alpha, ifn-gamma etc.) have been shown to increase the endothelial adhesiveness for human lymphocytes, including natural killer cells (nk cells). in this work we have demonstrated that pretreatment of either the target endothelial cell monolayers or the binding lgl-cells with mr595 salmonella minnesota bacteria results in a substantial increase in t ... | 1991 | 1710821 |
| lysis of neisseria gonorrhoeae initiated by binding of normal human igm to a hexosamine-containing lipooligosaccharide epitope(s) is augmented by strain-specific, properdin-binding-dependent alternative complement pathway activation. | we studied the specificity of naturally acquired igm bactericidal for strains of neisseria gonorrhoeae that varied in sensitivity to the lytic action of normal human serum (nhs) and the relative ability of these strains to deplete the classical (cp) and alternative (acp) c pathways. lysis of both highly sensitive and relatively insensitive strains was inhibited by the same gonococcal lipooligosaccharides (los), as well as by salmonella minnesota re los and three hexosamine-containing glycose pol ... | 1991 | 1711080 |
| binding sites for endotoxins (lipopolysaccharides) on human monocytes. | the nature of the binding sites for lps on human monocytes was investigated using [3h] labeled intact lps from neisseria meningitidis and from salmonella minnesota r7, and the [3h] labeled purified inner core region (ps-ome) of s.m. r7 lps. in the presence of serum, intact lps from enterobacterial and nonenterobacterial strains bound to monocytes in a dose-dependent, saturable, and displaceable fashion. n.m. lps and lps from the enterobacterial strain of escherichia coli 0111-b4 bound to the sam ... | 1991 | 1716284 |
| lipopolysaccharide-induced stimulation of cd11b/cd18 expression on neutrophils. evidence of specific receptor-based response and inhibition by lipid a-based antagonists. | gram-negative bacterial septicemia is a common clinical syndrome resulting, in part, from the activation of phagocytic leukocytes by lps. by using flow cytometry, we have characterized lps-induced expression of the beta 2 integrin cd11b/cd18. after exposure to salmonella minnesota r595 lps, expression of neutrophil cd11b/cd18 is rapidly upregulated, beginning within 5 min and achieving a peak fluorescence (typically two- to threefold over base line) by 30 min. the increase in cd11b/cd18 expressi ... | 1991 | 1717586 |
| production of monoclonal antibodies specific for ganglioside gd3. | four kinds of anti-gd3 monoclonal antibodies, dsg-1, -2, -3, and -4, of the igm class were obtained by the immunization of balb/c mice with enzootic bovine leukosis tumor tissue-derived ganglioside gd3 inserted into liposomes with salmonella minnesota r595 lipopolysaccharides. the specificities of the monoclonal antibodies obtained were defined by complement-dependent liposome immune lysis assay and by enzyme immunostaining on thin-layer chromatography. the reactivities of the monoclonal antibod ... | 1991 | 1724444 |
| in vitro formation of complement activation products by lipopolysaccharide chemotypes of salmonella minnesota. | we have applied immunoassays for complement activation products c4d, fragment bb and the protein s-c5b-9 neoantigen (s-mac) to assess activation of classical, alternative and terminal pathways, respectively, by lipopolysaccharides (lps) from the smooth strain (ss) of salmonella minnesota and the shallow rough (core) mutants r60, r345, r5 and r7. incubations of sera (n = 6) with lps generated small and insignificant quantities of bb and s-mac in the case of rb, rc and rd chemotypes and slightly g ... | 1991 | 1752696 |
| aminoglycosides modify the in vitro metachromatic reaction and murine generalized shwartzman phenomenon induced by salmonella minnesota r595 lipopolysaccharide. | endotoxin-neutralizing activity may be an important property for antibiotics to be used in severe sepsis. several antibiotics, belonging to different classes, were evaluated as to their endotoxin-neutralizing ability, using the inhibition of an in vitro metachromatic assay for lipopolysaccharides and a murine generalized shwartzman reaction model. gentamicin, amikacin, and sisomicin have been found to share significant in vitro antiendotoxin activity at an antibiotic/endotoxin ratio as low as 1. ... | 1991 | 1759842 |
| endotoxin and lipid peroxidation in vivo in selenium- and vitamin e-deficient and -adequate rats. | the effect of salmonella typhimurium endotoxin injected intraperitoneally (0.5 mg/kg body weight) on lipid peroxidation in vivo was assessed. peroxidation was monitored by measuring ethane production, an autoxidation product of (n-3) unsaturated fatty acids. weanling rats were fed a selenium- and vitamin e-deficient basal diet or one supplemented with 0.2 mg se/kg and/or 200 mg vitamin e/kg. after 11 to 13 wk of feeding, ethane production was tripled in lps-treated se- and vitamin e-deficient ra ... | 1991 | 1995793 |
| disialoganglioside gd2 in human neuroectodermal tumor cell lines and gliomas. | monoclonal antibodies (mabs) recognizing the disialoganglioside ii3(neuac)2ggose3cer (gd2) were produced by immunizing mice with the gd2-expressing neuroblastoma cell line lan-1 and a prefusion boost with purified gd2 coupled to salmonella minnesota. two igm mabs were isolated which demonstrated high levels of reactivity (binding ratios in excess of 100) with gd2 by solid-phase radioimmunoassay and positivity in high-performance thin-layer chromatography (hptlc) immunostain; only one (dmab-20) w ... | 1991 | 1659106 |
| endotoxin-neutralizing properties of the 25 kd n-terminal fragment and a newly isolated 30 kd c-terminal fragment of the 55-60 kd bactericidal/permeability-increasing protein of human neutrophils. | the bactericidal/permeability-increasing protein (bpi) of polymorphonuclear leukocytes (pmn) is a potent cytotoxin, specific for gram-negative bacteria, that also inhibits endotoxin activity by neutralizing isolated bacterial lipopolysaccharides (lps). we have previously shown that an isolated 25 kd n-terminal fragment of human bpi carries all the antibacterial activities of the parent 55-60 kd molecule. in this study we have compared the lps-neutralizing activities of human holo-bpi, the n-term ... | 1991 | 1875165 |
| analysis of unmodified endotoxin preparations by 252cf plasma desorption mass spectrometry. determination of molecular masses of the constituent native lipopolysaccharides. | nine unmodified endotoxin preparations constituted of re-, rd-, and rc-type lipopolysaccharides (2 to 5 glycoses), representing four species of enterobacteria were analyzed by 252cf plasma desorption mass spectrometry. the constituent lipopolysaccharides were characterized by the ion pair: (m-h)- and its corresponding lipid fragment ion. the lipid fragment ion is produced by cleavage of the glycosidic bond of the 3-deoxy-d-manno-oct-2-ulosonic acid unit that substitutes o-6' of the glucosamin be ... | 1991 | 1917976 |
| neutralization of shwartzman-inducing activity by antibodies recognizing the re core or lipid a structures of lipopolysaccharide from salmonella minnesota r595 and pseudomonas vesicularis jcm1477. | antibodies recognizing the re core or lipid a structures of lipopolysaccharide (lps) derived from salmonella minnesota r595 and pseudomonas vesicularis jcm1477 were tested for the ability to neutralize the preparatory activity of endotoxin using the local shwartzman reaction. shwartzman-inducing activity of r595 lps (re-form) was strongly suppressed when the lps was incubated with the rabbit anti-r595 antiserum or the purified igg antibody which recognizes core region of the lps. the antiserum a ... | 1991 | 1921759 |
| bacterial lipopolysaccharide induces long-lasting iga deficiency concurrently with features of polyclonal b cell activation in normal and in lupus-prone mice. | polyclonal b cell activation (pba) and autoimmune disease can be induced in immunologically normal mice, or enhanced in lupus-prone mice, by bacterial lipopolysaccharide (lps). because immune defects are common in autoimmune diseases and iga deficiency is prevalent in patients with systemic lupus erythematosus, we investigated: (i) whether lps might induce iga deficiency in normal mice; (ii) whether iga deficiency might be a feature in lupus-prone mice; (iii) whether, if present in lupus-prone m ... | 1991 | 2015704 |
| inhibition by lipid a-specific monoclonal antibodies of priming of human polymorphonuclear leucocytes by endotoxin. | exposure to lipopolysaccharide (lps) primes polymorphonuclear leucocytes (pmnl) for enhanced release of oxygen metabolites after subsequent stimulation. the metabolic response of human pmnl primed with lps and stimulated with formyl-methionyl-leucyl-phenylalanine (fmlp) was measured by chemiluminescence (cl) as a parameter for endotoxic activity. polymyxin b (pmb) and monoclonal antibodies (mabs) with specificity for lipid a were tested for inhibition of the priming effect of re lps of salmonell ... | 1991 | 2020018 |
| monophosphoryl lipid a induces tolerance to the lethal hemodynamic effects of endotoxemia. | monophosphoryl lipid a (mpl) is a nontoxic derivative of lipid a. in this study, the induction of tolerance by mpl to the hemodynamic effects of lethal endotoxemia was tested. ten sprague-dawley rats were received either salmonella minnesota mpl 0.5 mg/100 g intravenously (i.v.) or equivalent volume of diluent (control) i.v. on day zero. on day 3 s. minnesota endotoxin (lps) 5.0 mg/100 g was administered i.v. cardiac output (co), arterial lactate (l), and central venous oxygen saturation (svo2) ... | 1991 | 2049817 |
| a new monoclonal antibody directed to sialyl alpha 2-3lactoneotetraosylceramide and its application for detection of human gastrointestinal neoplasms. | a new monoclonal antibody (ns24) directed to the n-acetylneuraminyl alpha 2-3gal beta 1-4glcnac residue in type ii sugar chain of n-acetylneuraminyllactoneotetraosylceramide [sialylparagloboside, iv3(neuac)nlc4cer] was prepared by hybridoma technique. liposomes composed of dipalmitoylphosphatidylcholine, cholesterol, iv3(neuac)nlc4cer, and lipopolysaccharides from salmonella minnesota r595 were used for immunization with iv3(neuac)nlc4cer isolated from human erythrocytes. this method allowed the ... | 1991 | 1864848 |
| lipopolysaccharide responsiveness is an important factor in the generation of optimal antigen-specific t cell responses during infection with gram-negative bacteria. | we previously have found that the endotoxin (lps) of gram-negative bacteria is a major determinant of macrophage ia induction during infection with these organisms. specifically, i.p. injection of gram-negative bacteria elicits a striking macrophage ia response in lps-responder mice but virtually no response in lps-low-responder mice. as an extension of these findings, in this report we have tested the hypothesis that the inability of lps-low responder mice to mount an ia response during gram-ne ... | 1991 | 1918965 |
| detection of lipopolysaccharide-binding proteins on membranes of murine lymphocyte and macrophage-like cell lines. | lipopolysaccharide-(lps) binding proteins present on murine-lymphocyte and macrophage-like cell lines were identified by a ligand-blotting method and subsequent immunological detection of bound lps. membrane proteins of the murine-pre-b-cell line 70z/3 were separated by sds-page, transferred electrophoretically onto nitrocellulose, and the blot was incubated with lps of the salmonella minnesota re-mutant r595 (mre-lps). lps bound to proteins on nitrocellulose was immunologically detected by anti ... | 1991 | 1725955 |
| protective effects of polyclonal sera and of monoclonal antibodies active to salmonella minnesota re595 lipopolysaccharide during experimental endotoxemia. | mice were passively immunized with sera from blood donors active for rough lipopolysaccharides (lps), the j5 (rc chemotype) mutant of escherichia coli o111:b4, and the re595 (re chemotype) mutant of salmonella minnesota. all protected the mice against lethal challenge with smooth e. coli wf96 lps, e. coli and salmonella rough mutant lps, or free lipid a. epitopes recognized by monoclonal antibodies (mabs) reacting with the lps of s. minnesota re595 or lipid a were localized in the 2-keto-3-deoxy ... | 1990 | 1700024 |
| binding of rough lipopolysaccharides (lps) to human leukocytes. inhibition by anti-lps monoclonal antibody. | the binding of rough lps (relps from salmonella minnesota r595) to human peripheral blood polymorphonuclear leukocytes (pmn), monocytes, and lymphocytes was examined by using fluorescein-labeled lps and flow cytometry. at 4 degrees c, fitc-relps bound rapidly in a concentration- and time-dependent way to pmn, monocytes, and lymphocytes. because mononuclear cells showed both binding and nonbinding cell populations, fitc-relps was used in conjunction with specific phycoerythrin-labeled mab to iden ... | 1990 | 2162891 |
| monophosphoryl lipid a inhibits neutrophil priming by lipopolysaccharide. | it is known that lipopolysaccharides (endotoxin) prime neutrophils for oxygen radical production. monophosphoryl lipid a is a nontoxic derivative of lipid a that protects against lethal endotoxemia. we examined the effects of salmonella minnesota monophosphoryl lipid a on s. minnesota lipopolysaccharide-induced priming of neutrophil superoxide anion generation. human neutrophils were preincubated with and without either lipopolysaccharide or monophosphoryl lipid a before stimulation with 10(-5) ... | 1990 | 2168464 |
| phase diagram of lipid a from salmonella minnesota and escherichia coli rough mutant lipopolysaccharide. | we have reported here on the structural polymorphism of lipid a, the "endotoxic principle" of bacterial lipopolysaccharide. for lipid a of rough mutant lipopolysaccharide from salmonella minnesota and escherichia coli, the three-dimensional supramolecular structures were determined with x-ray diffraction utilizing synchrotron radiation. the investigations were performed in the water concentration range 10 to 95% by weight, at [lipid a]:[mg2+] molar ratios from 1:0 to 0.1:1, and in the temperatur ... | 1990 | 2100144 |
| [stimulation of nonspecific antibacterial resistance of mice to opportunistic gram-negative microorganisms with triterpene glycosides from holothuroidea]. | a total fraction of triterpenic glycosides from cucumaria japonica named cucumarioside was used as a stimulator of nonspecific resistance to bacterial infections in mice. after intraperitoneal administration to mice subsequently infected with various strains of e. coli and proteus mirabilis, cucumarioside provided survival of 40 to 90 per cent of the infected animals against 100 per cent in the control group. the protective effect directly depended on the dose of cucumarioside. it was optimal to ... | 1990 | 2110443 |
| priming of polymorphonuclear granulocytes by lipopolysaccharides and its complexes with lipopolysaccharide binding protein and high density lipoprotein. | human peripheral blood neutrophils are primed, or enabled to respond to formyl peptide, by prior exposure to bacterial lipopolysaccharide (lps). the activity of lps and the size of its aggregates are altered by plasma constituents such as high density lipoprotein (hdl) and the recently discovered acute phase reactant lipopolysaccharide binding protein (lbp) tobias et al.: j. exp. med. 164,777, 1986]. the ability of lps, lps-lbp, and lps-hdl complexes to activate a number of cellular responses ha ... | 1990 | 2154525 |
| transfer of preformed terminal c5b-9 complement complexes into the outer membrane of viable gram-negative bacteria: effect on viability and integrity. | an efficient fusion system between gram-negative bacteria and liposomes incorporating detergent-extracted c5b-9 complexes has been developed that allows delivery of preformed terminal complexes to the cell envelope (tomlinson et al., 1989b). fusion of salmonella minnesota re595 and escherichia coli 17 with c5b-9-incorporated liposomes resulted in the transfer of 1900 c5b-9 complexes to each target bacterial cell. no loss in viability of bacteria was observed following fusion, even though the dep ... | 1990 | 2184889 |
| investigation into the fluidity of lipopolysaccharide and free lipid a membrane systems by fourier-transform infrared spectroscopy and differential scanning calorimetry. | the phase behaviour, particularly the fluidity within each phase state and the transitions between them, of lipopolysaccharides and of their lipid moiety, free lipid a, of various species of gram-negative bacteria, especially of salmonella minnesota and escherichia coli, has been investigated by applying mainly fourier-transform infrared spectroscopy and differential scanning calorimetry. for enterobacterial strains, the transition temperatures of the gel----liquid crystalline (beta----alpha) ph ... | 1990 | 2199198 |
| [proteus peritonitis-bacteremia in mice--a model for studying postvaccinal proteus immunity]. | the dynamics of the formation of postvaccinal immunity after immunization with preparations obtained with the use of hydroxylamine (ha) preparations from proteus strains of different o serogroups, salmonella minnesota re-mutant and the common antimicrobial antigen isolated from escherichia coli 14 has been studied on mice with proteus peritonitis-bacteremia used as a model. the study has revealed that intraperitoneal immunization with proteus ha preparations stimulates the phagocytic activity of ... | 1990 | 2251896 |
| isolation and characterisation of 3-deoxy-d-manno-2-octulopyranosonate 7-(2-aminoethyl phosphate) from the inner core region of escherichia coli k-12 and salmonella minnesota lipopolysaccharides. | the title compound (pe-kdo) was isolated after hydrolysis of the lipopolysaccharides of escherichia coli k-12 strain w3100 and salmonella minnesota strains r4 and r7, and the location of the 2-aminoethyl phosphate group at position 7 was established by 13c-n.m.r. spectroscopy. derivatives of pe-kdo were acetylated, silylated, and methylated in order to evaluate their usefulness for analysis by g.l.c.-m.s. | 1990 | 2279250 |
| electron microscopic observation of crystals of escherichia coli k-12 lipopolysaccharide. | previously we showed that salmonella minnesota re and ra lipopolysaccharides (lpss) and escherichia coli k-12 lps formed three-dimensional crystals, either hexagonal plates or solid columns, when they were precipitated by the addition of 2 volumes of 95% ethanol containing 375 mm mgcl2 and incubated in 70% ethanol containing 250 mm mgcl2, and stored at 4 c for 10 days. later, escherichia coli k-12 lps thus treated was found to form discoid crystals as well as hexagonal plate crystals and solid c ... | 1990 | 2280724 |
| characterization of murine monoclonal and murine, rabbit, and human polyclonal antibodies against chlamydial lipopolysaccharide. | murine monoclonal and rabbit, murine, and human polyclonal antibodies against chlamydial lipopolysaccharide (lps) were characterized by the passive hemolysis and passive hemolysis inhibition assays and by absorption experiments with lpss of chlamydia psittaci, chlamydia trachomatis, and a recombinant strain of salmonella minnesota re (r595-207) expressing the chlamydia-specific lps epitope, as well as natural and synthetic partial structures of chlamydial lps. eleven monoclonal antibodies of the ... | 1990 | 2294050 |
| decreased tumor necrosis factor production during the initial stages of infection correlates with survival during murine gram-negative sepsis. | secretion of tumor necrosis factor (tnf)/cachectin occurs during gram-negative bacterial sepsis in response to macrophage stimulation by lipopolysaccharide (endotoxin) and may play an early pivotal role in the subsequent host response. we sought to determine whether administration of: (1) murine monoclonal antibody directed against endotoxin, (2) steroids, or (3) antimicrobial agents would abrogate tnf production and whether the protective capacity would correlate with tnf levels in an experimen ... | 1990 | 2294880 |
| a comparison of specificity and biological activity of polyclonal and monoclonal antibodies raised against salmonella minnesota r595 lipopolysaccharide. | murine monoclonal antibodies (mabs) and immune rabbit serum were raised against the rough mutant of salmonella minnesota strain r595. these antibodies were tested for their ability to inhibit lps-induced b-cell mitogenicity and neutralise lps toxicity in chick embryos. immune rabbit serum inhibited both mitogenicity and lps lethality. none of the mabs or a cocktail of antibodies were able to neutralise lps lethality in chick embryos. however, they were able to inhibit mitogenicity by varying deg ... | 1990 | 2304067 |
| comparisons of pasteurella multocida lipopolysaccharides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine relationship between group b and e hemorrhagic septicemia strains and serologically related group a strains. | lipopolysaccharides (lpss) purified from 16 reference somatic serotypes of pasteurella multocida were examined and compared by discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis. resolution of lps patterns in a gel was optimum when sample wells were cast separately from the stacking gel and the running gel consisted of 15% t (total monomer) polyacrylamide and 4 m deionized urea. band patterns of p. multocida lpss in a gel differed from control salmonella minnesota wild-type ... | 1990 | 2332462 |
| endotoxic lipid a interaction with human platelets. structure-function analysis of lipid a homologs obtained from salmonella minnesota re595 lipopolysaccharide. | we previously reported that human blood platelets are directly stimulated by endotoxic lipid a via the protein kinase c pathway (grabarek, j., timmons, s., and hawiger, j. (1988) j. clin. invest. 82, 964-971). to study the relationship between the molecular structure of lipid a and its ability to activate human platelets, we used lipid a homologs derived from salmonella minnesota re595 lipopolysaccharide. preparations of lipid a are heterogeneous in regard to the degree of substitution of fatty ... | 1990 | 2335520 |