Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [effects of panax notoginseng saponins on long-term potentiation in the ca1 region of the rat hippocampus]. | panax notoginseng saponins (pns) are very important extracts from roots of medicinal herb sanchi ginseng which is highly regarded in china for its therapeutic ability to meliorate blood-circulation, anti-anoxia, improve memory, and anti-caducity effects. in this study, we used blind whole-cell voltage-clamp recordings to detect the effects of pns on long-term potentiation (ltp) in the ca1 region of the hippocampus, and investigated the electrophysiological mechanisms underlying potentiating effe ... | 2007 | 18300467 |
| preparation of the traditional chinese medicine compound recipe shuxiong sustained-release capsules by multiparticulate time-controlled explosion technology. | in this study the traditional chinese medicine compound recipe (tcmcr) shuxiong sustained-release capsules (sxsrc) were prepared by multiparticulate time-controlled explosion technology. first, shuxiong pellets were prepared with the refined medicinal materials containing in the recipe of shuxiong tablets. then, the pellets were coated sequentially with an inner swelling layer containing low-substituted hydroxypropylcellulose as the swelling agent and an outer rupturable layer of ethylcellulose. ... | 2007 | 17557747 |
| chromatographic behaviour of steroidal saponins studied by high-performance liquid chromatography-mass spectrometry. | the chromatographic behaviour of steroidal saponins found in anemarrhena asphodeloides, asparagus officinalis, convallaria majalis, digitalis purpurea and ruscus aculeatus was studied by hplc-ms using a c-18 reversed-phase column and aqueous acetonitrile or aqueous methanol mobile phase gradients, with or without the addition of 1% acetic acid. the behaviour was compared to that of triterpene saponins found in aesculus hippocastanum, centella asiatica, panax notoginseng and potentilla tormentill ... | 2007 | 17391684 |
| analysis of chemical and metabolic components in traditional chinese medicinal combined prescription containing radix salvia miltiorrhiza and radix panax notoginseng by lc-esi-ms methods. | high-performance liquid chromatography-electrospray ionization-mass spectrometry (lc-esi-ms) methods were developed for the analysis of chemical and metabolic components in traditional chinese medicinal combined prescription containing radix salvia miltiorrhiza and radix panax notoginseng (commonly known as fufang danshen prescription, fdp). the hplc experiments used a reversed-phase zorbax c(18) column with the column temperature at 30 degrees c and a binary mobile phase system consisting of aq ... | 2007 | 17428004 |
| [effect of total panax notoginseng saponins on inducing differentiation of mononuclear cells in human cord blood into endothelial cells]. | to investigate the effect of total panax notoginseng saponins (tpns) to induce the differentiation of mononuclear cells (mnc) in cord blood into endothelial cells, the dmem culture media containing tpns were used to induce the mnc of cord blood. then, the morphology of the adherent cells was observed by the light microscopy and the fluorescence microscopy, the changes of cell surface markers (uea-1), function marker (vwf) and cd31 were detected by flow cytometry. the results showed that the numb ... | 2007 | 17490549 |
| [effects of panax notoginseng saponins on mrna expressions of interleukin-1 beta, its correlative factors and cysteinyl-aspartate specific protease after cerebral ischemia-reperfusion in rats]. | to investigate the effects of panax notoginseng saponins (pns) on mrna expressions of interleukin-1 beta (il-1 beta), interleukin-1 receptor type i (il-1ri), interleukin-1 receptor antagonist (il-1ra), intercellular adhesion molecule-1 (icam-1), cysteinyl-aspartate specific protease-1 (caspase-1), caspase-3 and caspase-8 after cerebral ischemia-reperfusion in rats. | 2007 | 17498496 |
| immunological adjuvant effect of ginsenoside rh4 from the roots of panax notoginseng on specific antibody and cellular response to ovalbumin in mice. | ginsenoside rh(4) (1), a saponin isolated from the roots of panax notoginseng (burk.) f. h. chen, was evaluated for its haemolytic activity and adjuvant potential on specific antibody and cellular response to ovalbumin (ova) in mice. compound 1 showed a slight haemolytic effect, its concentration inducing 50% of the maximum haemolysis (hd(50) value) being 407+/-12 microg/ml using a 0.5% suspension of red blood cells. compound 1 significantly increased the concanavalin a (con a)-, lipopolysacchar ... | 2007 | 17311234 |
| induction of apoptosis in rat c6 glioma cells by panaxydol. | panaxydol is a naturally occurring non-peptidyl small molecule isolated from the lipophilic fractions of panax notoginseng, a well-known chinese traditional medicine. previous studies have shown that panaxydol inhibited the growth of various kinds of malignant cell lines. to date, there has been no report concerning the effect of panaxydol on cell growth inhibition in glioma cells. in this paper, we examined panaxydol's antiproliferation and proapoptotic effects on rat c6 glioma cells and invest ... | 2007 | 17320424 |
| ultra-performance liquid chromatography/time-of-flight mass spectrometry based metabolomics of raw and steamed panax notoginseng. | at present, metabolite profiling is of growing importance in herbal medicine fields such as breeding, formulation, quality control and clinical trials. this preliminary study indicated that ultra-performance liquid chromatography/time-of-flight mass spectrometry (uplc/tofms)-based metabolomics allows direct detection of down-stream derivatives of metabolites, arising from the herbal formulation process. this analytical approach allows the discrimination and tentative authentication of unique bio ... | 2007 | 17238214 |
| [clinical study on effect of total panax notoginseng saponins on immune related inner environment imbalance in rheumatoid arthritis patients]. | to study the therapeutic effect and possible mechanism of total panax notoginseng saponins (pns) for treatment of rheumatoid arthritis (ra), and to observe its safety and influence on ra immune related inner environment. | 2007 | 17717913 |
| improved quality control method for fufang danshen preparations through simultaneous determination of phenolic acids, saponins and diterpenoid quinones by hplc coupled with diode array and evaporative light scattering detectors. | a herb-combined prescription, mainly derived from roots of salvia miltiorrhiza and panax notoginseng, has been widely used for improving coronary or cerebral circulation in china as well as in western countries. multiple commercially available preparations, known as fufang danshen preparations (fdps), produced by various manufacturers with the raw materials from different sources, pose a serious challenge to the quality control of this herb medicine. previous pharmacological studies identify thr ... | 2007 | 17720349 |
| [effect of panax notoginseng saponins on serum neuronal specific enolase and rehabilitation in patients with cerebral hemorrhage]. | to observe the effect of panax notoginseng saponins (pns) on serum content of neuronal specific enolase (nse) and function recovery in patients with cerebral hemorrhage (ch) for exploring the therapeutic action of pns in treating ch. | 2007 | 17343007 |
| ginsenoside rd elicits th1 and th2 immune responses to ovalbumin in mice. | ginsenoside rd (rd), a saponin isolated from the roots of panax notoginseng, was evaluated for inducing th1 or th2 immune responses in mice against ovalbumin (ova). icr mice were immunized subcutaneously with ova 100 microg alone or with ova 100 microg dissolved in saline containing alum (200 microg), or rd (10, 25 or 50 microg) on days 1 and 15. two weeks later (day 28), concanavalin a (con a)-, lipopolysaccharide (lps)- and ova-stimulated splenocyte proliferation was determined using mtt assay ... | 2007 | 16950547 |
| effects of aqueous extracts of aconitum carmichaeli, rhizoma bolbostemmatis, phytolacca acinosa, panax notoginseng and gekko swinhonis gūenther on bel-7402 cells. | to investigate the anticancer activity of a chinese medical mixture, wrcp (warming and relieving cold phlegm), on hepatocarcinoma bel-7402 cells. | 2007 | 17569146 |
| notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells. | panax notoginseng is a commonly used chinese herb. although a few studies have found that notoginseng shows anti-tumor effects, the effect of this herb on colorectal cancer cells has not been investigated. 5-fluorouracil (5-fu) is a chemotherapeutic agent for the treatment of colorectal cancer that interferes with the growth of cancer cells. however, this compound has serious side effects at high doses. in this study, using hct-116 human colorectal cancer cell line, we investigated the possible ... | 2007 | 17009031 |
| analysis of dencichine in panax notoginseng by gas chromatography-mass spectrometry with ethyl chloroformate derivatization. | dencichine (beta-n-oxalyl-l-alpha,beta-diaminopropionic acid) is a haemostatic agent present in well-known traditional chinese medicinal herbs such as panax notoginseng, as well as other panax species. it is also a reported neurotoxic agent found in lathyrus sativus (grass pea seed) and cycad seeds. a method was developed for quantitative determination of the non-protein amino acid, dencichine, in plant samples of p. notoginseng and the adventitious roots directly from the explants of p. notogin ... | 2007 | 17029672 |
| simultaneous determination of seven active components of fufang danshen tablet by high performance liquid chromatography. | a high-performance liquid chromatography method was established for simultaneously determining seven major components, i.e. protocatechuic aldehyde, notoginsenoside r(1), ginsenoside rg(1), salvianolic acid b, ginsenoside rb(1), cryptotanshinone and tanshinone iia in fufang danshen tablet, a commonly used traditional chinese medicinal combined prescription mainly derived from the roots of salvia miltiorrhiza and panax notoginseng. these seven compounds, belonging to the chemical types of phenoli ... | 2007 | 17120299 |
| inhibitory effect of panax notoginseng on nitric oxide synthase, cyclo-oxygenase-2 and neutrophil functions. | a water extract of panax notoginseng buck f.h. chen. (arialiaceae) root (pn) is being used as a therapeutic agent to stop haemorrhages and as a tonic to promote health in korean and chinese medicine. the pharmacokinetic profiles of pn have not been accurately investigated. the preliminary aim was to elucidate the pharmacokinetic features of pn. first, the prevention of neutrophil functions was assessed. pn inhibited neutrophil functions, including degranulation, superoxide generation and leukotr ... | 2007 | 17128437 |
| [studies on chemical constituents from rhizomes of panax notoginseng]. | to study the chemical constituents from rhizomes of panax notoginseng. | 2007 | 18323204 |
| [optimization of biotransformation conditions of active component in panax notoginseng stalks and leaves by fusarium sacchari]. | by using fusarium sacchari, a rare microbial strain isolated and screened from planted ginseng soil, the active component notoginseng triterpenes in panax notoginseng stalks and leaves was biotransformed. taking three main anti-tumor components, i. e., 20 (s)-protopanoxadiol-20-o-beta-d-glucopyranose (compound k, c-k), 20-o-beta-d-xylopyranosyl (1 --> 6) -beta-d-glucopyranosyl-20 (s)-protopanaxadiol (compound mx, c-mx) and 20 (s)-protopanoxadiol-20-o-alpha-l-arabofuranose (1 --> 6)-beta-d-glucop ... | 2007 | 18333458 |
| simultaneous determination of 11 saponins in panax notoginseng using hplc-elsd and pressurized liquid extraction. | a new hplc coupled with evaporative light scattering detection (elsd) method was developed for simultaneous determination of 11 major triterpene saponins, namely notoginsenoside r1 (1), ginsenosides rg1 (2), re (3), rf(4), rb1 (5), rg2 (6), rc (7), rb2 (8), rb3 (9), rd (10), and rg3 (11) in panax notoginseng, a commonly used traditional chinese medicine (tcm). pressurized liquid extraction (ple) was employed for sample preparation, and the analysis was achieved using a zorbax ods c18 column elut ... | 2006 | 17069249 |
| ginsenoside-rd from panax notoginseng blocks ca2+ influx through receptor- and store-operated ca2+ channels in vascular smooth muscle cells. | previously, it was found that total saponins from panax notoginseng inhibited ca2+ influx coupling to activation of alpha1-adrenoceptor. this study was designed to investigate the effects of ginsenoside-rd from total saponins of panax notoginseng on receptor-operated (rocc) and store-operated (socc) ca2+ channels in vascular smooth muscle cells using fura-2 fluorescence, whole cell patch clamp ion channel recording, radio-ligand-receptor binding, 45ca2+ radio-trace and organ bath techniques. it ... | 2006 | 16973156 |
| [effects of ethanol extracts of panax notoginseng on liver metastasis of b16 melanoma grafted in mice]. | to observe the effects of ethanol extracts of panax notoginseng on the tumor and the liver metastasis in experimental mice grafted with b16 melanoma. | 2006 | 16965745 |
| [protective effects of saponines of stem and leaf of panax notoginseng on acute myocardial ischemia in anaesthetic dogs]. | to study the protective effects of saponines of stem and leaf of panax notoginseng (pnssl) on acute myocardial ischemia in anaesthetic dogs. | 2006 | 16548173 |
| oral absorption of ginsenoside rb1 using in vitro and in vivo models. | this research attempts to clarify the cause for poor oral absorption of ginsenoside rb1 (rb1), one main ingredient of the well known panax notoginseng saponins (pns) for curing hemorrhage. caco-2 cell monolayers were used as an in vitro model to reveal the transport mechanism of rb1 across the intestinal mucosa. moreover, the serum concentration-time profiles of rb1 after tail venous (iv), portal venous (pv), intraduodenal (id) and peroral (po) administration to rats were compared to evaluate th ... | 2006 | 16557452 |
| notoginsenoside r1 inhibits tnf-alpha-induced fibronectin production in smooth muscle cells via the ros/erk pathway. | the matrix fibronectin protein plays an important role in vascular remodeling. notoginsenoside r1 is the main ingredient with cardiovascular activity in panax notoginseng; however, its molecular mechanisms are poorly understood. we report that notoginsenoside r1 significantly decreased tnf-alpha-induced activation of fibronectin mrna, protein levels, and secretion in human arterial smooth muscle cells (hasmcs) in a dose-dependent manner. notoginsenoside r1 scavenged hydrogen peroxide (h2o2) in a ... | 2006 | 16632126 |
| mechanism of altered tnf-alpha expression by macrophage and the modulatory effect of panax notoginseng saponins in scald mice. | to explore the mechanism of altered tumor necrosis factor-alpha (tnf-alpha) expression by peritoneal macrophages (pmphi) and panax notoginseng saponins (pns) modulation in light of nf-kappab signal transduction in severely scalded mice. | 2006 | 16814471 |
| detection of genetic homogeneity of panax notoginseng cultivars by sequencing nuclear 18s rrna and plastid matk genes. | the nuclear 18s rrna and chloroplast matk genes of 18 samples of panax notoginseng and its processed material sanqi (radix notoginseng) were analyzed. the two genes, regardless of cultivar origin, were found to be identical to genotype r1 and m1, respectively, of the published sequences (genbank accession no. d85171 and ab027526). this phenomenon implies that the species is highly conserved, which is probably caused by the use of the same strain in cultivation and the lack of active mutation in ... | 2006 | 16881018 |
| ginsenoside re and notoginsenoside r1: immunologic adjuvants with low haemolytic effect. | the further purification of the total saponins from the roots of panax notoginseng (burk.) f. h. chen by ordinary and reversed-phase silica-gel, as well as sephadex lh-20 chromatography afforded two adjuvant active dammarane-type saponins, ginsenoside re (1) and notoginsenoside r1 (2). these two saponins were evaluated for haemolytic activities and adjuvant potentials on the cellular and humoral immune responses of icr mice against ovalbumin (ova). the concentrations inducing 50% of the maximum ... | 2006 | 17193304 |
| a uniform hplc method developed for the analysis of salvia miltiorrhiza, panax notoginseng, and fufang danshen. | fufang danshen (ffds) is a famous typical chinese complex prescription, which is mainly composed of radix salvia miltiorrhiza bunge (sm) and radix panax notoginseng (pn). an hplc method is developed to analyze sm, pn, and ffds effectively; the effective analysis is achieved by using a gradient elution procedure with a mobile phase consisting of acetonitrile and 0.025% aqueous phosphoric acid (v/v). through this method, 33 peaks in ffds are clearly exhibited, and the components that make up the 3 ... | 2006 | 17254367 |
| [rp-hplc characteristics of panax notoginseng]. | to develop the chemical characteristics of panax notoginseng in order to control its quality. | 2006 | 17262953 |
| [analysis of dencichine by hplc with pre-column derivatization]. | to establish a reversed-phase high performance liquid chromatorgraphy (rp-hplc) method for detecting the dencichine in panax notoginseng extracts and drug preparations. | 2006 | 17285985 |
| [experimental study on anti-platelet effects of ginsenoside -2a in vitro]. | to explore the in vitro anti-platelet effects of ginsenoside -2a,a purified extract from panax notoginseng. | 2006 | 17569356 |
| a rapid hplc-esi-ms/ms for qualitative and quantitative analysis of saponins in "xuesetong" injection. | 'xuesetong' injection, one of the most widely used proprietary medicines in traditional chinese medicine, consists of total saponins made from panax notoginseng, which is a highly valued and important chinese medicinal herb. it is used to treat cardiovascular diseases. in order to control the quality of xuesetong injection, a rapid hplc-esi-ms/ms method was developed for qualitative and quantitative determination of the saponins. the analyses were performed on sb-c18 column using gradient elutio ... | 2006 | 16343839 |
| panax notoginseng attenuates lps-induced pro-inflammatory mediators in raw264.7 cells. | herbals or dietary supplements are not regulated as drugs by the united states food and drug administration (usfda) although many may have associated therapeutic effects and toxicities. therefore, the immunomodulatory effects of the herbal extract panax notoginseng on cultured macrophages (raw264.7 cells) were investigated to address potential therapeutic or toxic effects. cells were stimulated with lps (1 microg/ml) and treated with notoginseng at 5, 25 and 50 microg/ml. notoginseng inhibited t ... | 2006 | 16427227 |
| separation and on-line concentration of saponins from panax notoginseng by micellar electrokinetic chromatography. | a simple, reproducible and sensitive micellar electrokinetic chromatography (mekc) method was developed for the separation and determination of 10 saponins from panax notoginseng. field-enhanced sample injection with reverse migrating micelles (fesi-rmm) was used for on-line concentration of the saponins. the effects of concentrations of sodium dodecyl sulfate (sds) and organic modifier, the sample matrix, the injection time of water plug, the injection voltage and injection time of sample on th ... | 2006 | 16469324 |
| solid-phase extraction and liquid chromatography-electrospray mass spectrometric analysis of saponins in a chinese patent medicine of formulated salvia miltiorrhizae and panax notoginseng. | in this paper, a method using solid-phase extraction (spe) and hplc/esi-ms(n) for the identification of the major saponins in "danshen dripping pill", a chinese patent medicine consisting of salvia miltiorrhizae and panax notoginseng, is described. through solid-phase extraction process, the saponins in "danshen dripping pill" were separated from the phenolic constituents of s. miltiorrhizae and, meanwhile, efficiently concentrated. subsequently, these saponins were characterized by hplc/esi-ms( ... | 2006 | 16111853 |
| extraction of 20(s)-ginsenoside rg2 from cultured panax notoginseng cells in vitro stimulates human umbilical cord vein endothelial cell proliferation. | from the cultured cells of panax notoginseng, a kind of ginseng saponin-ginsenoside rg2, was separated and purified, and its structure was elucidated as 6-o-[alpha-l-rhamnose (l-->2)-beta-d-glucopyranosyl]3beta, 12beta, 20(s)-trihydroxydammar-24-ene [20(s)-ginsenoside rg2]. moreover, its bioactivity stimulates human umbilical cord vein endothelial cell proliferation; the growth of the cells treated with 20(s)-ginsenoside rg2 was 14-fold higher than that of untreated ones, and the level of the ce ... | 2006 | 16772761 |
| [inhibitory effect of panax notoginseng on the vsmc proliferation induced by hyperlipidemia serum]. | to investigate the effect of p. notoginseng on vascular smooth muscle cell (vsmc) proliferation induced by hyperlipidemia serum. | 2006 | 16780166 |
| high-performance liquid chromatographic assay for the active saponins from panax notoginseng in rat tissues. | a reversed-phase liquid chromatographic method was used to determine the ginsenosides rg1, rb1 and rd of panax notoginseng in rat tissues (kidney, liver, heart, spleen and lung) after the administration of total saponins of p. notoginseng. the tissue samples were treated with solid-phase extraction prior to hplc. the calibration curves for the three saponins were linear in the given concentration ranges. the intra-day and inter-day assay coefficients in tissues were between 76 and 120% respectiv ... | 2006 | 16138297 |
| pharmacological activity of sanchi ginseng (panax notoginseng). | the pharmacological activity and constituents of the sanchi ginseng panax notoginseng have been reviewed. the bulk of pharmacological findings have been based on the saponins or steryl glycosides, although polysaccharides with immunopotentiating activity, proteins with antifungal, ribonuclease and xylanase activity, and a triacylglycerol (trilinolein) with antioxidant activity have been reported. protective actions against cerebral ischaemia, beneficial effects on the cardiovascular system, and ... | 2006 | 16872547 |
| difference in oral absorption of ginsenoside rg1 between in vitro and in vivo models. | to clarify the cause of poor oral absorption of ginsenoside rg1 (rg1), the active ingredient in panax notoginseng saponins (pns) used for treating hemorrhage. | 2006 | 16539852 |
| efficient induction of ginsenoside biosynthesis and alteration of ginsenoside heterogeneity in cell cultures of panax notoginseng by using chemically synthesized 2-hydroxyethyl jasmonate. | chemically synthesized 2-hydroxyethyl jasmonate (heja) was for the first time employed to induce the ginsenoside biosynthesis and to manipulate the product heterogeneity in plant cell cultures. the dose response and timing of heja elicitation were investigated in cell suspension cultures of panax notoginseng. the optimal concentration and timing of heja addition for both cell growth and ginsenoside accumulation was identified to be 200 microm added on day 4. it was interestingly found that heja ... | 2006 | 16041570 |
| compound salvia pellet, a traditional chinese medicine, for the treatment of chronic stable angina pectoris compared with nitrates: a meta-analysis. | compound salvia pellet (csp), consisting of active herbal ingredients extracted from danshen (salvia miltiorrhiza), sanqi (panax notoginseng), and borneol (cinnamomum camphora), is taken most frequently by patients with angina pectoris in china. to determine the efficacy and safety of csp for the treatment of stable angina pectoris (sap), a meta-analysis was undertaken. | 2006 | 16369479 |
| panaxynol induces neurite outgrowth in pc12d cells via camp- and map kinase-dependent mechanisms. | panaxynol, a polyacetylene ((3r)-heptadeca-1,9-diene-4,6-diyn-3-ol; syn. falcarinol), was isolated from the lipophilic fractions of panax notoginseng, a chinese traditional medicinal plant. in the present study, we reported the neurotrophic effects of panaxynol on pc12d cells and mechanism involved in neurite outgrowth of the cells. panaxynol could morphologically promote neurite outgrowth in pc12d cells, concentration-dependently reduce cell division and up-regulate molecular marker (map1b) exp ... | 2006 | 16219303 |
| utilization study of stems and leaves of tienchi ginseng. i. anti-hypertensive effect of stems and leaves of tienchi ginseng on stroke-prone spontaneously hypertensive rat (shrsp). | tienchi ginseng tea was prepared from stems and leaves of tienchi ginseng which is a special product in china. the increase in systolic blood pressure (sbp) was significantly inhibited by the consumption of a 4% this tea solution as drinking water from the prehypertensive stage (6 weeks of age) in male stroke-prone spontaneously hypertensive rats (shrsp). a similar intake from the hypertensive stage also showed an anti-hypertensive effect. in contrast, a similar intake had no effect on sbp of no ... | 2006 | 17031036 |
| immunoactive polysaccharide-rich fractions from panax notoginseng. | panax notoginseng is a commonly used medicinal plant in south-western china. in a previous study, a sequential solubilisation of p. notoginseng high-molecular-weight (hmw) polymers using phenol-acetic acid-water, hot water, weak and strong alkali was performed to determine the structure of the component polysaccharides and proteins. the effects of these extracted hmw fractions on the human complement system, polymorphonuclear neutrophils (pmn) and peripheral blood mononuclear cells (pbmc) are re ... | 2006 | 16981127 |
| [effect of panax notoginseng saponins injection on brain edema in intracerebral hemorrhage rats]. | to study the effect of panax notoginseng saponins (pns) in treating hemorrhagic apoplexy at super-early stage in rats. | 2006 | 17121046 |
| [adventitious root induction and in vitro culture of panax notoginseng]. | to investigate the induction and culture of adventitious root of panax notoginseng. | 2006 | 17144461 |
| haemolytic activity and adjuvant effect of notoginsenoside k from the roots of panax notoginseng. | notoginsenoside k (1), a saponin isolated from the roots of panax notoginseng (burk.) f. h. chen, was evaluated for its haemolytic activity and adjuvant potential on specific antibody and cellular response to ovalbumin (ova) in mice. compound 1 showed a slight haemolytic effect, its concentration inducing 50% of the maximum haemolysis (hd50 value) being 318+/-13 microg/ml, on a 0.5% suspension of red blood cells. compound 1 significantly increased the concanavalin a (con a)-, lipopolysaccharide ... | 2006 | 17193229 |
| ginsenoside rd from panax notoginseng is cytotoxic towards hela cancer cells and induces apoptosis. | the saponin ginsenoside rd (1), isolated from panax notoginseng, is used for the treatment of cardiovascular diseases, inflammation, different body pains, trauma, and internal and external bleeding due to injury. in this study, we report that 1 inhibits the cell growth of human cervical cancer (hela) cells in a concentration- and time-dependent manner, with an ic(50) value of 150.5+/-0.8 mcirog/ml after 48 h of incubation. the drug-treated cells displayed features of apoptosis, including typical ... | 2006 | 17193257 |
| simultaneous determination of nine saponins from panax notoginseng using hplc and pressurized liquid extraction. | a hplc and pressurized liquid extraction (ple) method was developed for simultaneous determination of nine saponins, including notoginsenoside r1, ginsenoside rg1, re, rf, rb1, rc, rb2, rb3 and rd in panax notoginseng. the analysis was performed on c18 column with water-acetonitrile gradient elution and the investigated saponins were authenticated by comparing retention time and mass spectra with their reference compounds. several methods including ple, ultrasonication, soxhlet extraction and im ... | 2006 | 16316736 |
| structure and biological activity of protopanaxatriol-type saponins from the roots of panax notoginseng. | the further purification of the total saponins from the roots of panax notoginseng by using ordinary and reversed-phase silica-gel, as well as sephadex lh-20 chromatography afford seven adjuvant active protopanaxatriol-type saponins (pts), ginsenosides-rh1 (rh1),-rh4 (rh4),-rg1 (rg1),-re (re), notoginsenosides-r1 (r1),-r2 (r2),-u (u). these saponins were evaluated for their haemolytic activities and adjuvant potentials on the cellular and humoral immune responses of icr mice against ovalbumin (o ... | 2006 | 16332509 |
| notoginsenoside r1 from panax notoginseng inhibits tnf-alpha-induced pai-1 production in human aortic smooth muscle cells. | notoginsenoside r1 is the main ingredient with cardiovascular activity in panax notoginseng. we reported that notoginsenoside r1 significantly decreased tumor necrosis factor-alpha (tnf-alpha)-induced plasminogen activator inhibitor-1 (pai-1) mrna, protein level and secretion in human aortic smooth muscle cells (hasmcs) in a dose-dependent manner. tnf-alpha significantly increased extracellular signal-related kinases (erk) and protein kinase b (pkb) activation and, which was blocked by notoginse ... | 2006 | 16458614 |
| [determination of organochlorine pesticide residue in nine chinese herbs by gas chromatography]. | to determine organochlorine pesticide residue in 9 chinese herbs. | 2006 | 16495190 |
| chemical characteristics for different parts of panax notoginseng using pressurized liquid extraction and hplc-elsd. | the chemical characteristics for different parts of panax notoginseng, including root, fibre root, rhizome, stem, leaf, flower and seed, were determined using high performance liquid chromatography-evaporative light scattering detection (hplc-elsd) and pressurized liquid extraction (ple). eight major saponins, namely notoginsenoside r1, ginsenosides rg1, re, rb1, rc, rb2, rb3 and rd were also quantitatively compared among the different parts of p. notoginseng. the chromatograms showed that there ... | 2006 | 16522361 |
| isolation of a ribonuclease from sanchi ginseng (panax pseudoginseng) flowers distinct from other ginseng ribonucleases. | a single-chained ribonuclease was isolated from the aqueous extract of sanchi ginseng (panax pseudoginseng) flowers. it exhibited a molecular mass of 23 kda, an n-terminal sequence with some similarity to other enzymes involved in rna metabolism but different from known ribonucleases, and considerably higher activity toward poly u than poly c and only slight activity toward poly a and poly g. the purification protocol entailed ion exchange chromatography on diethylaminoethyl (deae)-cellulose, af ... | 2006 | 16540087 |
| effect of panax notoginseng saponins on lipopolysaccharide-induced adhesion of leukocytes in rat mesenteric venules. | panax notoginseng is the root of the chinese traditional herb, panax notoginseng (burk) f.h. chen. this study was aimed to investigate the inhibitory effect of panax notoginseng saponins (pns) on the leukocyte adhesion and the expression of adhesion molecules in rat mesentery venules. male sprague-dawley rats were anesthetized with urethane. these were divided into control, lps (perfused with lipopolysaccharide), and pns group (perfused with pns). the mesenteric microcirculation was observed und ... | 2006 | 16543624 |
| attenuating effect of myakuryu on mesenteric microcirculatory disorders induced by ischemia and reperfusion. | myakuryu (mr) is a newly developed herbal medicine composing crataegue oinnatifida bge (cob), panax notoginseng (pn) and ginkyo biloba (gb). to examine the effectiveness of mr, we investigated its effects on rat mesenteric microcirculatory injury induced by ischemia/reperfusion (i/r). the mesenteric microcirculation of ileocecal portion of a male wistar rat was observed through an inverted-type intravital microscope assisted with a charge-coupled devise (ccd) camera. mesenteric i/r was conducted ... | 2006 | 16543630 |
| [effects of astragalus membranaceus and panax notoginseng on the transformation of bone marrow stem cells and proliferation of epc in vitro]. | to investigate the effect and the possible mechanism underlying the promotional effect of astragalus membranaceus and panax notoginseng on the transformation of bone narrow stem cells and proliferation of epc. | 2005 | 16468374 |
| induction and characterization of adventitious roots directly from the explants of panax notoginseng. | adventitious roots from leafstalks and lateral roots were obtained directly from explants of panax notoginseng. the lateral root explants were more sensitive to the induction of adventitious roots using indole-3-butyric acid. hplc analysis of saponins extracted from the adventitious roots indicated that several protopanaxatriol saponins were present but ginsenoside rd was missing, compared with the saponins extracted from the raw herbs. the dry weight of primary adventitious root culture of pana ... | 2005 | 16314969 |
| genetic diversity and variation of saponin contents in panax notoginseng roots from a single farm. | radix notoginseng, the root of panax notoginseng (burk.) f. h. chen, has been widely used in traditional chinese medicine. its main components, saponins, have been reported to have many pharmacological activities. to test the general assumption that herbs of a single species planted and harvested from a single location are uniform in chemical and genetic makeup, chemical analysis and dna fingerprinting were carried out. high-performance tlc together with hplc analysis were used to analyze 17 ran ... | 2005 | 16248538 |
| determination of four active saponins of panax notoginseng in rat feces by high-performance liquid chromatography. | a method is developed for the determination of ginsenoside rg1, rb1, rd, and notoginsenoside r1 of panax notoginseng (pns) in rat feces after oral and intravenous administration of total saponins of pns. the fecal samples are treated with organic extraction and solid-phase extraction prior to high-performance liquid chromatography. the calibration curves for the four saponins are linear in the given concentration ranges. the precision of the method is in the range of 1.0-10.0% (relative standard ... | 2005 | 16212783 |
| identification of panax species in the herbal medicine preparations using gradient pcr method. | in order to identify the existence of panax species in herbal medicine preparations, the ginseng specific marker primer was selected and created based on the sequence of korean ginseng dna fragment, 359 bp. the gradient pcr was performed on 40 types of the herbal medicines including the 7 types of araliaceae that are in the same family with the panax ginseng using the created ginseng maker primer. as result, panax notoginseng (chinese), panax japonicus (japanese) and panax quinquefolius (america ... | 2005 | 15802808 |
| inhibitory effect of trilinolein on endothelin-1-induced c-fos gene expression in cultured neonatal rat cardiomyocytes. | trilinolein, isolated from the traditional chinese herb sanchi (panax notoginseng), has been shown to have myocardial protective effects via its antioxidant ability. however, the cellular and molecular mechanisms of the protective effect of trilinolein in the heart remain to be elucidated. oxidative mechanisms have been implicated in neonatal cardiomyocyte hypertrophy. we previously reported that et-1 induces ros generation via the et(a) receptor and ros modulates c-fos gene expression. we have ... | 2005 | 16184402 |
| [effects of panax notoginseng saponins on rat cardiomyocytes apoptosis induced by angiotengin ii in vitro]. | to study the protective effects of panax notoginseng saponins (pns) on angiotensin ii (ang ii)-induced rat cardiomyocyte apoptosis in vitro and the probable mechanism. | 2005 | 16075720 |
| [effects of the panax notoginseng saponins on the level of synaptophysin protein in brain in rat model with lesion of meynert]. | to observe the protective effect of panax notoginseng saponins (pns) on the level of synaptophysin ptotein in brain in rat model with alzheimer's disease (ad). | 2005 | 16124609 |
| orthogonal array design in optimizing the extraction efficiency of active constituents from roots of panax notoginseng. | the root of panax notoginseng (radix notoginseng, sanqi) is a commonly used traditional chinese medicine, which is mainly cultivated in wenshan of yunnan china. the identified active constituents in radix notoginseng include saponin, ssavonoid and polysaccharide; however, the levels of these active constituents vary greatly with different extraction processes. this variation causes a serious problem in standardizing the herbal extract. by using hplc and spectrophotometry, the contents of notogin ... | 2005 | 16177971 |
| [effect of panax notoginseng saponins on intercellular adhesion molecule-1 expression and neutrophil infiltration in cerebral infarction tissue of rats]. | to investigate the effect of panax notoginseng saponins (pns) on the expression of intercellular adhesion molecule-1 (icam-1) and brain neutrophil infiltration induced by transient focal cerberal ischemia and reperfusion in rats. | 2005 | 16131033 |
| enhancement of ginsenoside biosynthesis in high-density cultivation of panax notoginseng cells by various strategies of methyl jasmonate elicitation. | a single addition of 200 microm methyl jasmonate (mja) to high-density cell cultures of panax notoginseng enhanced ginsenoside production in both shake-flask (250 ml) and airlift bioreactor (alr; 1 l working volume). repeated elicitation with two additions of 200 microm mja during cultivation further induced the ginsenoside biosynthesis in both cultivation vessels. the content of ginsenosides rg1, re, rb1 and rd in the alr was increased from, respectively, 0.18+/-0.01, 0.21+/-0.01, 0.21+/-0.02 a ... | 2005 | 15630581 |
| relationship between haemolytic and adjuvant activity and structure of protopanaxadiol-type saponins from the roots of panax notoginseng. | four protopanaxadiol-type saponins (pds), ginsenosides-rb(1), -rd, notoginsenosides-k, -r(4) isolated from the roots of panax notoginseng were evaluated for their haemolytic activities and adjuvant potentials on the cellular and humoral immune responses of icr mice against ovalbumin (ova). the effect of the substitution pattern of these pds on their biological activities was investigated and structure-activity relationships were established. among four pds, the ranking of the haemolytic activity ... | 2005 | 16115706 |
| [analysis of saponins from panax notoginseng using pressurized solvent extraction coupled with liquid chromatography-electrospray mass spectrum]. | to establish a method for qualitative analysis of saponins from panax notoginseng using pressurized solvent extraction coupled with lc-esi-ms. | 2005 | 16479924 |
| [relationship between the inhibitory effects of pns on cardiac hypertrophy and its action on neurohormonal factor]. | to study the effects of total saponins of panax notoginseng (pns) on the overall model and vitro model of cardiac hypertrophy, and investigate the mechanism of its action. | 2005 | 16124610 |
| [the pharmacokinetics and pharmacodynamics of intranasal preparation of panax notoginseng saponins]. | to investigate the pharmacokinetic course of intranasal powders of panax notoginseng saponins (pns) in a rat model and its protective effects against cardio-cerebrovascular diseases administrated in the form of its suspension. | 2005 | 16011272 |
| absorption profiles of sanchinoside r1 and ginsenoside rg1 in the rat intestine. | panax notoginseng is used as a therapeutic agent in chinese medicine for stopping hemorrhage and also as a general health remedy. although panax notoginseng saponins (pns) are currently attracting attention due to their hemorheological properties, the absorption profiles of pns have still not been fully investigated. in the present study, an in situ intestinal perfusion rat model was used to investigate the absorption mechanism of sanchinoside r1 (r1) and ginsenoside rg1 (rg1), two main componen ... | 2005 | 16435571 |
| [protective effects of panax notoginseng saponins against pathological lesion of cholinergic neuron in rat model with alzheimer' s disease]. | to observe the protective effect of panax notoginseng saponins (pns) against pathological lesion of cholinergic neuron in rat model with alzheimer' s disease (ad). | 2005 | 15981887 |
| up-regulation of m1 muscarinic receptors expressed in chom1 cells by panaxynol via camp pathway. | loss of cholinergic neurons along with muscarinic acetylcholine receptors (machrs) in cerebral cortex and hippocampus is closely associated with alzheimer's disease (ad). recent drug development for ad treatment focuses heavily on identifying m(1) receptor agonists. however, machrs undergo down-regulation in response to agonist-induced sustained activation. therefore, therapeutic effectiveness wanes during continuous use. thus, another potentially effective approach, which overcomes this drawbac ... | 2005 | 15936523 |
| molecular mechanism of the inhibitory effect of trilinolein on endothelin-1-induced hypertrophy of cultured neonatal rat cardiomyocytes. | trilinolein, isolated from the traditional chinese herb sanchi ( panax notoginseng), has been shown to have myocardial protective effects via its antioxidant ability. however, the cellular and molecular mechanisms of the protective effect of trilinolein in the heart remain to be elucidated. oxidative mechanisms have been implicated in neonatal cardiomyocyte hypertrophy. we therefore have examined whether trilinolein attenuates reactive oxygen species (ros) production and thus et-1-induced hypert ... | 2005 | 15971123 |
| [apoptosis of rat hepatic stellate cells induced by panax notoginseng saponins]. | 2005 | 15727717 | |
| [apoptosis-promoting effect of panax notoginseng extracts on mnng-transformed ges-1 cells]. | to study the apoptosis-promoting effect of the serum from panax notoginseng extracts-fed dog on precancerous gastric cells by means of flow cytometry. | 2005 | 15763060 |
| [analysis on the geologic background and physicochemical properties of soil for the cultivation of panax notoginseng in yunnan province]. | to study the relationship between the geo-authentic and geologic background system (gbs) and physicochemical properties of soil for the cultivation of panax notoginseng. | 2005 | 15806962 |
| hydrophilic interaction liquid chromatography with tandem mass spectrometry for the determination of underivatized dencichine (beta-n-oxalyl-l-alpha,beta-diaminopropionic acid) in panax medicinal plant species. | dencichine (beta-n-oxalyl-l-alpha,beta-diaminopropionic acid) is a haemostatic agent present in important chinese medicinal herbs such as panax notoginseng, as well as other panax species. it is also a reported neurotoxic agent found in lathyrus sativus (grass pea seed). a selective analytical method incorporating hydrophilic interaction chromatography with positive electrospray ionization tandem mass spectrometry (hilic/esi-ms/ms), for the analysis of dencichine in panax plant species, was deve ... | 2005 | 15838924 |
| characterization of cell wall polysaccharides from the medicinal plant panax notoginseng. | panax notoginseng is a commonly used medicinal plant in south-western china. recent studies indicate that wall polysaccharides are responsible for some of the immunostimulatory activity. fractionation of the p. notoginseng root powder alcohol insoluble residue (air) and its compositional analysis enabled us to deduce the polysaccharide and protein composition of the root cell walls. p. notoginseng walls are composed primarily of polysaccharide (approximately 97% w/w) and some protein. the polysa ... | 2005 | 15896377 |
| simultaneous quantification of six major active saponins of panax notoginseng by high-performance liquid chromatography-uv method. | a simple, sensitive and specific high-performance liquid chromatography-uv (hplc-uv) method has been developed for the first time to simultaneously quantify the six major active saponins of panax notoginseng, namely notoginsenoside r1, ginsenoside rg1, rb1, rg2, rh1 and rd. astragaloside iv is used as the internal standard. this hplc assay was performed on a reversed-phase c18 column with gradient elution of acetonitrile and 0.01% formic acid in 30 min. the method provided good reproducibility a ... | 2005 | 15907618 |
| panax notoginseng saponins preconditioning protects rat liver grafts from ischemia/reperfusion injury via an antiapoptotic pathway. | ischemia/reperfusion (i/r) injury is a major cause of primary graft dysfunction and renders an allograft more immunogenic in orthotopic liver transplantation (olt). panax notoginseng saponins (pns) has been reported to exert protective effects against i/r injury to various organs. the objective of this study is to investigate whether pns preconditioning protects rat liver grafts from i/r injury via an antiapoptotic pathway. | 2005 | 15908317 |
| [effects of panax notoginseng saponins on the process of renal interstitial fibrosis after unilateral ureteral obstruction in rats]. | to evaluate the effects of panax notoginseng saponins (pns) on the process of renal interstitial fibrosis after unilateral ureteral obstruction in rats. | 2005 | 15931871 |
| ginsenoside-rd from panax notoginseng enhances astrocyte differentiation from neural stem cells. | neural stem cells cultured as neurospheres were used to assess the effects of p. notoginseng on the production of neurons and glia. the crude saponins (pns) and ginsenoside-rd promote the differentiation of neurospheres into astrocytes. ginsenoside-rd increases the production of astrocytes in a dose-dependent manner. on the other hand, both pns and ginsenoside-rd induce a weak but significant effect by decreasing the number of neurons. the other ginsenosides do not induce any differentiation on ... | 2005 | 15607328 |
| a dammarane glycoside derived from ginsenoside rb3. | a dammarane glycoside, designated compound mx (c-mx), was isolated from the hydrolysate of 20(s)-protopanaxadiol type ginsenosides containing g-rb(3) from panax notoginseng leaves with crude snailase. its chemical structure was elucidated to be 20-o-beta-d-xylopyranosyl(1-->6)-beta-d-glucopyranosyl-20(s)-protopanaxadiol on the basis of spectral analysis. its cytotoxicity against breast cancer cell line mcf-7 and effects on the sensitivity to doxocubicin of doxocubicin-resistant mcf-7 cells were ... | 2005 | 15684516 |
| impact of external calcium and calcium sensors on ginsenoside rb1 biosynthesis by panax notoginseng cells. | the effects of external calcium concentrations on biosynthesis of ginsenoside rb1 and several calcium signal sensors were quantitatively investigated in suspension cultures of panax notoginseng cells. it was observed that the synthesis of intracellular ginsenoside rb1 in 3-day incubation was dependent on the medium ca2+ concentration (0-13 mm). at an optimal ca2+ concentration of 8 mm, a maximal ginsenoside rb1 content of 1.88 +/- 0.03 mg g(-1) dry weight was reached, which was about 60% and 25% ... | 2005 | 15627250 |
| a korean herbal medicine, panax notoginseng, prevents liver fibrosis and hepatic microvascular dysfunction in rats. | we assessed the prevention of hepatic fibrogenesis by water-extract of panax notoginseng buck f.h. chen. (arialiaceae) root (pns) in long-evans rats with cinnamon coat color (lec rats). lec rats were divided into three groups a, fed on a basal diet (bd); b, fed on bd plus 1% pns; and c), fed on bd plus 0.005% lycopene as a control. all rats were sacrificed at 26 weeks of age. the percentage of the total area involved by fibrosis was 1.46 +/- 0.47 in group a, 0.83 +/- 0.10 in b (p=0.0030, b vs a) ... | 2005 | 15698847 |
| immunological-adjuvant saponins from the roots of panax notoginseng. | the total saponin extract from the dried roots of panax notoginseng (burk.) f. h. chen possesses immunological-adjuvant activities. guided by in vivo immunological tests, further study on this fraction afforded three active dammarane-type saponins. their structures were determined on the basis of chemical evidence and extensive spectroscopic methods, including 1d- and 2d-nmr. the novel compound (20s)-protopanaxatriol 20-o-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranoside (1), and the two know ... | 2005 | 17192000 |
| panax notoginseng supplementation enhances physical performance during endurance exercise. | the purpose of this study was to investigate whether a single 1,350-mg dose of panax notoginseng (png) could enhance aerobic capacity, endurance, and mean blood pressure (map) in young adults. we randomly assigned 29 untrained adults, aged 20-35 years, to an experimental (exp, n = 13) or a control (con, n = 16) group. for 30 days, the exp took 1,350 mg per day of png capsule and the con consumed 1,350 mg per day of starch capsule. measurement variables were taken before and after 30 days of eith ... | 2005 | 15744902 |
| [effect of radix notoginseng saponins on platelet activating molecule expression and aggregation in patients with blood hyperviscosity syndrome]. | in order to explore the relationship between the active components and the functional links of chinese herbs, the effect of xuesaitong capsule, a preparation made of multi-component panax notoginseng saponins (pns) on platelet activating molecule expression and aggregation in patients with blood hyperviscosity syndrome (bhs) was observed, with aspirin (asp) as a control. | 2004 | 15143716 |
| [study on therapeutic window of opportunity for panax notoginseng saponins following focal cerebral ischemia/reperfusion injury in rats]. | to study the therapeutic window of opportunity for panax notoginseng saponins (pns) following focal cerebral ischemia/reperfusion injury in rats. | 2004 | 15179785 |
| the antioxidant effects of aqueous and organic extracts of panax quinquefolium, panax notoginseng, codonopsis pilosula, pseudostellaria heterophylla and glehnia littoralis. | the roots of panax quinquefolium, panax notoginseng, glehnia littoralis, codonopsis pilosula and pseudostellaria heterophylla were extracted with an aqueous extraction method and also with an organic extraction method. the aqueous extracts of glehnia littoralis and codonopsis pilosula were the most potent in inhibiting erythrocyte hemolysis. the aqueous extracts of panax quinquefolium and panax notoginseng had lower potencies while the aqueous extract of pseudostellaria heterophylla and the orga ... | 2004 | 15234766 |
| liquid chromatographic method for determination of four active saponins from panax notoginseng in rat urine using solid-phase extraction. | four major active saponins (ginsenosides rg1, rb1, rd and notoginsenoside r1) in panax notoginseng were determined in rat urine after oral and intravenous administration of total saponins of p. notoginseng (pns), and the urine samples were treated with solid-phase extraction (spe) prior to liquid chromatography. a reversed-phase liquid chromatography system with ultraviolet detection and a zorbax sb-c18 column was used. the within-day and between-day assay coefficients of variation for the four ... | 2004 | 15261811 |
| total saponins of panax notoginseng protected rabbit iliac artery against balloon endothelial denudation injury. | to investigate whether total panax notoginseng saponins (pns) could protect endothelium of rabbit iliac artery against balloon endothelial denudation (bed) injury. | 2004 | 15339390 |
| adjuvant effect of panax notoginseng saponins on the immune responses to ovalbumin in mice. | in this study, the haemolytic activities of panax notoginseng saponins (pns) and its adjuvant potentials on the cellular and humoral immune responses of icr mice against ovalbumin (ova) were evaluated. we determined the haemolytic activity of pns using 0.5% rabbit red blood cell. pns showed a slight haemolytic effect, with its haemolytic percents being 11.59 and 3.60% at the concentrations of 500 and 250 microg/ml, respectively. furthermore, the adjuvant potential of pns at three dose levels on ... | 2004 | 15364435 |
| hplc determination of four active saponins from panax notoginseng in rat serum and its application to pharmacokinetic studies. | four main active saponins (ginsenosides rg1, rb1, rd and notoginsenoside r1) in panax notoginseng in rat serum after oral and intravenous administration of total saponins of p. notoginseng (pns) to rats were determined using a simple and sensitive high-performance chromatographic method. the serum samples were pretreated with solid-phase extraction before analysis. the calibration curves for the four saponins were linear in the given concentration ranges. the intra-day and inter-day assay coeffi ... | 2004 | 15484230 |
| [effect of total panax notoginseng saponins on the activity of human endothelial nitric oxide synthase gene promoter]. | to study the mechanism of total panax notoginseng saponin (tpns) in regulating the transcription activity of human endothelial nitric oxide synthase (henos) gene promoter. | 2004 | 15485778 |