Publications

antitumoral activity of an immunomodulatory fraction of nocardia opaca: mechanism of action.	immunomodulatory substances have been used as antineoplastic agents in experimental and human systems. many of these agents were derived from microorganisms. several biologically active fractions have been isolated from nocardia. these derivatives were shown to induce interferon production, to activate natural killer cells and macrophages and to exert an antitumoral effect. we attempted to examine the mechanism of the antitumoral activity of the nocardia water-soluble mitogen (nwsm). the tumor t ...	1994	7927996
treatment of radiation disease by nocardia fraction: possible effect of inflammatory cytokines.	nocardia delipidated cell mitogen (ndcm) derived from nocardia opaca, given 2 h after whole-body gamma irradiation (2.5 gy) to germ-free piglets in amounts of 1 mg/kg via a stomach tube, prevented the loss of b-cells in the spleen, intestinal mucosa, and mesenteric lymph nodes when recorded on day 8 after irradiation. ndcm and/or formolized enterococcus faecalis strain (ef) applied intraperitoneally also 2 h after irradiation increased the survival rate in mice irradiated with 8.3 gy and 8.5 gy ...	1994	7927997
induction of inflammatory cytokines by nocardia fractions.	ndcm and nld fractions of nocardia opaca cell walls were used for in vitro stimulation of human and porcine peripheral blood mononuclear cells. inflammatory cytokines il-1, il-6, tumour necrosis factor (tnf) alpha and interferon (ifn) gamma were detected at the single-cell level using paraformaldehyde-fixed and saponin-permeabilized mononuclears stained with cytokine-specific antibodies and indirect immunofluorescence method. il-1, il-6 and tnf were produced by human monocytes stimulated for 2 h ...	1993	8206169
nocardia fractions, nld and nwsm, induce tumor necrosis factor-alpha secretion in human monocytes: role of protein kinase c.	nocardia lysozyme digest (nld) and nocardia water-soluble mitogen (nwsm) are two fractions derived from nocardia opaca. in this report, we demonstrated that both fractions elicited significant secretion of tumor necrosis factor-alpha (tnf-alpha) in human monocytes. supernatants from monocytes stimulated with nwsm and low concentrations of nld displayed a cytotoxic activity against tnf-alpha-sensitive l929 cells, but supernatants from monocytes stimulated with high concentrations of nld failed to ...	1993	8325335
improved purification of steroid 1:2-dehydrogenase from nocardia opaca and partial characterization of its cloned gene sequence.	we have purified a steroid-inducible 1:2-dehydrogenase from nocardia opaca. the final enzyme preparation was purified 120-fold with a recovery of 38%. the n-terminal amino acid sequence was determined to be: met-gln-asp-trp-thr-ser-glu-(cys)-asp-val-leu-val-val-gly-. from the genomic library of nocardia opaca in the plasmid puc19, a clone designated as pstd23 containing a 0.9 kb kpni-psti fragment was found to hybridize with an oligonucleotide probe corresponding to the first six amino acids fro ...	1993	7916596
changes in distribution of lectin receptors in macrophages activated by nocardia water soluble molecules.	lectin-histochemical studies were performed on macrophages activated by nocardia opaca water soluble molecules (nwsm) and elicited by phosphate buffered saline (pbs) as control to demonstrate the pattern of carbohydrate residues. ten different biotinylated lectins were used as probes and avidin-biotin-peroxidase complex (abc) was used as visualizer. the lectins wheat germ agglutinin (wga) and griffonia simplicifolia agglutinin-i (gs-i) stained macrophages only after activation by nwsm and not ma ...	1993	8298432
the giant linear plasmid phg207 from rhodococcus sp. encoding hydrogen autotrophy: characterization of the plasmid and its termini.	as described previously, in rhodococcus sp. (formerly nocardia opaca) strains mr11 and mr22, the ability to grow as an aerobic hydrogen bacterium (the aut character) is located on giant conjugative linear plasmids--on phg201 (270 kb) in strain mr11 and on phg205 (280 kb) in strain mr22. in an autotrophic transconjugant originating from mr22 a smaller plasmid, phg207 (225 kb), was detected and shown to be a recombination product of the wild-type plasmids phg204 and phg205. a donor carrying phg207 ...	1993	8245832
immunomodulatory activities of nocardia opaca.	nocardia lysozyme digest (nld), a particulate fraction from nocardia opaca, is able to induce antitumor activity to sal-1 tumor cells (lung sarcoma) in balb/c mice. in mice immunized with nld inhibition of tumor growth and prolonged survival of tumor bearing animals was observed. macrophages isolated from peritoneal cavity and stimulated with nld release a few arachidonic acid metabolites, mostly pge 2. macrophages from tumor bearing mice are more sensitive to nocardia antigens than normal. both ...	1993	7509560
phagocytic response modifying reactivity of enzymatic cell wall digests of nocardia opaca.	aqueous extracts (enocw) and enzymatic digests of purified nocardia opaca cell wall fragments, virtually free of muramyl peptides, were monitored for their phagocytic response modifying reactivity on polymorphonuclear leucocytes, separated or unseparated in whole human blood. in the presence of enocw a 74% increased production of superoxide during the respiratory burst of tpa-activated polymorphonuclear leukocytes was observed, as compared to the unprimed control. delipidation of this preparatio ...	1990	2177449
hydrogen autotrophy of nocardia opaca strains is encoded by linear megaplasmids.	several linear megaplasmids were detected in the facultatively lithoautotrophic gram-positive bacterium nocardia opaca. the wild-type strain mr11 contains, in addition to the cccdna plasmids phg31-a and phg31-b, the linear plasmids phg201 (270 kb), phg202 (400 kb) and phg203 (420 kb). the wild-type strain mr22 contains, in addition to the cccdna plasmid phg33, the linear plasmids phg204 (180 kb), phg205 (280 kb) and phg206 (510 kb). after preparation of dna from cells embedded in agarose, the li ...	1990	2200846
intrinsic b lymphocyte defect in untreated patients with hodgkin's disease.	in vivo and in vitro humoral and cell-mediated immunological defects have been described in untreated patients with hodgkin's disease (hd). the cellular basis of the recently described in vitro reduction of mitogen-induced immunoglobulin synthesis has not been elucidated so far. in this study, we attempted to dissect t and b lymphocyte function in untreated hd patients. mitogen-induced in vitro immunoglobulin synthesis was assessed in the presence of pokeweed mitogen, the mitogenic anti-cd3 mono ...	1990	2261600
comparison of the nh2-terminal amino acid sequences of the four non-identical subunits of the nad-linked hydrogenases from nocardia opaca 1b and alcaligenes eutrophus h16.	the cytoplasmic, nad-linked hydrogenase of the gram-positive hydrogen-oxidizing bacterium nocardia opaca 1b was compared with the analogous enzyme isolated from the gram-negative bacterium alcaligenes eutrophus h16. the hydrogenase of n. opaca 1b was purified by a new procedure applying chromatography on phenyl-sepharose and deae-sephacel with two columns in series. a homogeneous enzyme preparation with a specific activity of 74 mumol h2 oxidized.min-1.mg protein-1 and a yield of 32% was isolate ...	1989	2496982
enhancement of natural killer cell activity by nocardia opaca fractions.	three molecules derived from nocardia opaca bacteria, ndcm, nwsmp, and pg, have been shown to express immunomodulating properties. the present study was aimed at assessing the effects of these derivatives on natural killer (nk) activity. two experimental protocols were adopted, consisting of incubating whole or percoll fractionated nk cells in vitro with those substances, and the other in which the derivatives were administered in vivo to mice and the activity assessed later. incubation of splee ...	1989	2466328
application of chromatographic methods to the analysis of macrophage factors induced by nocardia opaca cell walls.	cell walls from nocardia opaca induce the production of mitogenic factors by mouse peritoneal macrophages in vitro. these factors stimulate thymocytes from c3h/hej mice. supernatants of peritoneal cell culture exhibiting this activity were fractionated by chromatographic procedures such as gel filtration and metal chelate affinity chromatography and the biological activities assayed. these fractionation studies indicate that several biologically active products occur in the supernatant. four fac ...	1988	3403659
suppression of human iga-b-cell maturation by iga-binding factors requires t cells.	iga-binding factors (iga-bfs) prepared from human peripheral blood mononuclear cells selectively decrease the generation of cytoplasmic iga-positive cells in peripheral blood mononuclear cell cultures stimulated by pokeweed mitogen or by nocardia opaca delipidated cell mitogen. in n. opaca delipidated cell mitogen stimulated cultures, the suppressive effect of iga-bfs was no longer demonstrable after removal of sheep erythrocyte rosetting t cells or after depletion of cd8+ cells, but it was not ...	1988	3265931
stimulation of sea star asterias rubens axial organ b-like cells by nocardia-delipidated cell mitogen and derived fractions.	the axial organ (ao-cells) of the starfish asterias rubens is a primitive immune organ. the total population was fractionated into two populations: adherent (b-like) and non-adherent (t-like) to nylon wool. nocardia-delipidated cell mitogen (ndcm), nocardia water soluble mitogen (nwsm), nwsm-pellet (nwsmp) and purified cell walls (cw) from nocardia opaca induce the proliferation of b-like cells from the axial organ. the nocardia immunomodulators studied are inactive on the total and t-like popul ...	1988	3203818
repeated batch production of l-phenylalanine from phenylpyruvate and nh(4)cl by immobilized cells of nocardia opaca under hydrogen high pressure.	among various microbial cells examined under screening conditions, nocardia opaca showed the highest activity for production of phenylalanine from phenylpyruvate. here nh(4)cl as well as amino acids were used as an amino donor for phenylalanine production. the phenylalanine production rate increased with increasing hydrogen pressure. the specific activity of phenylalanine dehydrogenase was increased by culturing n. opaca cells in nutrient broth containing 0.3% phenylalanine. as a result, the phe ...	1988	18584688
antimetastatic effect of immunomodulators from nocardia opaca in mice and rats activation of peritoneal macrophages by these fractions.	nocardia delipidated cell mitogen (ndcm), a particulate fraction prepared from nocardia opaca, injected i.p. in an oil/water emulsion to f6 rhabdomyosarcoma-bearing rats, inhibited the development of pulmonary metastases; 6 out of 10 rats were protected. repeated i.p. administration of emulsified ndcm and of two other compounds, a nocardia water soluble mitogen (nwsm a hydrosoluble fraction) and purified cell walls (cw, an insoluble macromolecular fraction) in lewis lung carcinoma (llc)-bearing ...	1987	3117366
nocardia water soluble mitogen (nwsm), a human b-lymphocyte mitogen.	nocardia water soluble mitogen (nwsm), which is a peptidoglycan, was prepared from nocardia opaca. its effect on human b-lymphocyte proliferation, the number of immunoglobulin secreting b-cells, and bone marrow stem cell growth was studied in vitro. nwsm stimulated the proliferation of b-lymphocytes of all ten healthy subjects studied very effectively (p less than 0.0005). no t-cell activation occurred. nwsm did not increase the amount of antibody producing cells. the growth of bone marrow stem ...	1986	3492453
no correlation exists between the conjugative transfer of the autotrophic character and that of plasmids in nocardia opaca strains.	a new isolate of nocardia opaca was obtained by enrichment culture for aerobic lithoautotrophic growth on co2 and h2. this strain, mr22, is very similar to n. opaca mr11 (formerly 1b) in functioning as a donor for genetic information determining the ability to grow lithoautotrophically (aut character) in matings with aut- strains of n. opaca or closely related heterotrophic species. the strain contains a plasmid, phg33 of about 110 kb. a mutant was isolated from strain mr22 which was plasmid-fre ...	1986	3463670
tumor localization and biodistribution of 99mtc-muramylpolypeptide (mpp) in the rat.	muramylpolypeptide, extracted from nocardia opaca bacteria and originally designed for immunotherapy of malignant tumors, proved to localize in malignant tumors, when labeled with 99mtc. in the tumor-bearing rat 99mtc-muramylpolypeptide (mpp) showed a fast turnover and optimum tumor to soft tissue ratios were obtained as early as between 30-60 min after application. the uptake mechanism in malignant tumors remains uncertain.	1986	3714507
distribution of ig classes and igg subclasses among human b cells activated by nocardia opaca-delipidated cell mitogen or by pokeweed mitogen.	the relative proportions of cells synthesizing the three major ig classes or one of the four igg subclasses in cultures stimulated with pokeweed mitogen (pwm) or nocardia-delipidated cell mitogen (ndcm) were investigated. in cultures of human peripheral blood mononuclear cells (pb mnc) stimulated with pwm, the number of igg-containing cells (cc) was higher than the number of igm-cc, and a substantial number of iga-cc was found. conversely, in ndcm-stimulated pb mnc cultures igm-cc outnumbered ig ...	1985	3875142
stimulation of human b lymphocytes by nocardia-delipidated cell mitogen and derived fractions from n. opaca. structure-activity relationship.	nocardia-delipidated cell mitogen (ndcm), a particulate fraction prepared from nocardia opaca, is able to stimulate the proliferation of small resting human b lymphocytes and their differentiation into ig-secreting cells. this fraction contains two active structures: the cell wall peptidoglycan (pg) and a fraction (cy i) derived from the cytoplasmic compartment. treatment of insoluble pg with various bacteriolytic enzymes showed that the minimal structure required for mitogenic activity is more ...	1985	3930601
a novel lymphokine derived from human igg fc receptor-bearing b cells: its suppressive effect on activated t and b lymphocytes including tumour cells in vitro.	peripheral blood fcr gamma-bearing human b cells, but neither t cells nor adherent cells, produce an immunoregulatory lymphokine after receiving the stimulation of fcr gamma by immune complexes--antibody-sensitized erythrocytes (ea). this factor suppresses polyclonal immunoglobulin (ig) production of b cells to pokeweed mitogen (pwm) and nocardia opaca delipidated cell mitogen (ndcm), indicating that not only b cells, but also t cells are targets for this factor. it also inhibits the proliferati ...	1985	3160654
suppression of polyclonal b cell activation by igg-binding factors. requirement for t cells.	igg-binding factors (igg-bf) prepared from cell-free supernatant of human peripheral blood mononuclear cells interfere with the polyclonal activation of peripheral b cells by decreasing the numbers of igg-containing cells and ig plaque-forming cells. using nocardia opaca delipidated cell mitogen (ndcm), a t helper cell-independent polyclonal b cell activator, it was found that the suppressive effect of igg-bf was no longer demonstrable after removal of t cells. in pokeweed mitogen-stimulated cul ...	1985	3155689
effect of nickel on activity and subunit composition of purified hydrogenase from nocardia opaca 1 b.	the nad-reducing hydrogenase of nocardia opaca 1 b was found to be a soluble, cytoplasmic enzyme. n. opaca 1 b does not contain an additional membrane-bound hydrogenase. the soluble enzyme was purified to homogeneity with a yield of 19% and a final specific activity of 45 mumol h2 oxidized min-1 mg protein-1. nad reduction with h2 was completely dependent on the presence of divalent metal ions (ni2+, co2+, mg2+, mn2+) or of high salt concentrations (0.5-1.5 m). the most specific effect was cause ...	1984	6319136
content and localization of fmn, fe-s clusters and nickel in the nad-linked hydrogenase of nocardia opaca 1b.	by preparative polyacrylamide gel electrophoresis at ph 8.5, and in the absence of nickel ions, two types of subunit dimers of the nad-linked hydrogenase from nocardia opaca 1b were separated and isolated, and their properties were compared with each other as well as with the properties of the native enzyme. the intact hydrogenase contained 14.3 +/- 0.4 labile sulphur, 13.6 +/- 1.1 iron and 3.8 +/- 0.1 nickel atoms and approximately 1 fmn molecule per enzyme molecule. the oxidized hydrogenase sh ...	1984	6086343
modulation of polyclonally activated human peripheral b cells by aggregated igg and by igg-binding factors: differential effect on igg subclass synthesis.	the regulation of igg subclass production by polyclonally activated human b cells was investigated by using two systems previously shown to selectively suppress the generation of igg-containing cells (cc) but not that of igmcc or igacc. the first one involved a brief exposure of peripheral blood mononuclear cells (pbmnc) to heat-aggregated human igg (agg-igg) followed by repeated washings and culture with untreated autologous pbmnc. the second one was achieved by addition of human igg-binding fa ...	1984	6610704
oxygen tolerance of strictly aerobic hydrogen-oxidizing bacteria.	growth of various bacteria, especially aerobic hydrogen-oxidizing bacteria, in the presence of 2 to 100% (v/v) oxygen in the gas atmosphere was evaluated. the bacterial strains included alcaligenes eutrophus, a. paradoxus, aquaspirillum autotrophicum, arthrobacter spec. strain 11 x, escherichia coli, arthrobacter globiformis, nocardia opaca, n. autotrophica, paracoccus denitrificans, pseudomonas facilis, p. putida, and xanthobacter autotrophicus. under heterotrophic conditions with fructose or g ...	1982	7049081
suppression of the late stages of mitogen-induced human b cell differentiation by fc gamma receptors (fc gamma r) released from polymorphonuclear neutrophils.	during short incubation in serum-free medium, polymorphonuclear neutrophils (pmn) release soluble material that can be characterized as receptors for fc igg (fc gamma r) on the following evidence: it agglutinates erythrocyte-igg antibody (eag) complexes, it prevents the binding of eag to eag-rosette-forming cells, and it binds to eag-rosette-forming cells after modulation of their fc gamma r, allowing the formation of 'passive' rosettes. these fc gamma r were isolated by affinity chromatography ...	1981	6456302
antitumor activity of intralesionally administered nocardia opaca preparations in rat and mouse tumors: a comparison with bcg and corynebacterium parvum.	three nocardia products: delipidated cells, lysozyme digest and nocardia water soluble mitogen (nw sm), have been assayed in regression experiments and compared with living bcg and killed c. parvum in rat and mouse fibrosarcomas transplanted intradermally. intratumor injection of these nocardia products induced regression in the bcg sensitive mcfifi2 (s) tumor. these substances were active at a dose of 1 mg, the lysozyme extract and nw sm also being active at 0.1 mg. association with mineral or ...	1981	7021434
[development of hydrogen bacteria on hard surfaces].	a simple model system stimulating the physical conditions of hydrogen bacterial growth in soil and loose rocks was studied. hydrogen bacterial cells grown under the autotrophic conditions were applied to the surface of coarse-grained silica gel, crushed brick and peat, and constantly supplied with a mixture of h2:o2:co2 (7:2:1). it was shown that nocardia opaca z-766, when cultivated on well-developed solid surfaces with a small quantity of mineral medium, produced an active bacterial film at th ...	1981	7242393
immunoregulatory functions of cultured human t lymphocytes.	twenty continuous cultures of human t cells (ctc) (15 from normal individuals and five from patients with t cell malignancy) growing in the presence of pha-stimulated lymphocyte conditioned medium were studied for their ability to participate in the regulation of the in vitro immunoglobulin (ig) production induced by five polyclonal activators, pokeweed mitogen (pwm), epstein-barr virus (ebv), nocardia opaca water-soluble mitogen (nwsm), streptolysin o (slo), and staphylococcl phage lysate (spl) ...	1980	6264658
suppression of mitogen-induced peripheral b cell differentiation by soluble fc gamma receptors released from lymphocytes.	soluble receptors for fcigg released from unstimulated human peripheral blood lymphocytes were isolated by affinity chromatography on sepharose 4b-igg. this material was shown to interfere with the differentiation of peripheral blood b cells into ig-secreting cells in cultures stimulated with pokeweek or nocardia opaca extracts. neither cell viability nor [3h]thymidine incorporation were altered, but the number of ig-containing cells and that of ig-secreting cells were decreased. these effects w ...	1980	7007063
[steroid transforming enzymes in microorganisms. xiii. relationship between steroid structure and induction of 4-ene-3-oxosteroid: (acceptor)-1-ene-oxidoreductase in nocardia opaca].	the induction of the 4-ene-3-oxosteroid: (acceptor)-1-ene-oxidoreductase in nocardia opaca has been shown to be dependent on both hydrophilic and hydrophobic structural features of the steroid molecule, i.g. the nature of the oxygen function in positions 11 and 20 and the presence of the methyl group in position 10 influence the induction of the enzyme drastically. in this connection the metabolism of the steroid inductors being degradated during the induction process has been considered.	1980	7376687
[certain features of the hydrogenase of the hydrogen bacterium nocardia opaca].		1980	7392989
[steroid-transforming enzymes from microorganisms. xii. induction characteristics of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase in nocardia opaca].	17 alpha-methyltestosterone and the corresponding 1(2)-dehydrocompound (dianabol) are efficient inducers of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca. after a lag period of 4 hours the enzyme activity increases rapidly. during the induction the steroids are completely metabolized causing a drastical drop of specific enzyme activity. using a fixed induction time the optimal steroid concentration and the temperature characteristic were found out. the influence of th ...	1979	547499
[isolation of an autotrophic variant of nocardia opaca].	a hydrogen-oxidizing autotrophic actinomycete, strain z-766, was isolated from the neuston of a marsh near moscow. the organism was identified as nocardia opaca z-766 according to its morphologo-physiological properties. the spectrum of organic compounds used by the strain, its cultural characteristics and growth are similar to those of the monotypical autotrophic microorganism n. opaca 1b described earlier. repeated isolation of the autotrophic variant of n. opaca z-766 suggests that the abilit ...	1979	155775
[steroid-transforming enzymes from microorganisms. xi. reversibility of the dehydrogenation reaction of the steroid-1-dehydrogenase from nocardia opaca].	highly purified preparations of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca have been investigated in both types of reactions: 1.2-dehydrogenation of the 4-en-3-oxo-derivative and 1.2-hydrogenation of the 1.4-dien-3-oxo-derivative. it was not possible to separate the hydrogenating activity from the dehydrogenating activity by affinity chromatography, disc electrophoresis, sds-electrophoresis, and isoelectric focusing techniques. the pure enzyme preparation is discus ...	1979	44773
[steroid-transforming enzymes from microorganisms. ix. affinity chromatographic preparation and studies of the apoenzyme from a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca].	from the flavoenzyme, 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase of nocardia opaca, prosthetic group and apoenzyme were separated quantitatively by means of affinity chromatography in the presence of 2 m (nh4)2 at ph 3.0. subsequently the apoenzyme was eluted from affinity matrix by 0.01 m phosphate buffer, ph 8.0, whereas under these conditions the intact enzyme could not be eluted. the whole enzyme activity applied could be restored by incubation of the eluted apoenzyme with fad. the bi ...	1979	44397
[interferon induced in the hamster by subunits of "nocardia": essay of protection against rabies (author's transl)].	lipid-free cells of nocardia opaca and a cell extract (nwsm) are interferon inducers in syrian hamsters: peak interferon appears 2 h after injection as compared to 8 h in the case of interferon induced by inactivated ndv virus. the interferon induced by nocardia product is inactivated by a treatment for 30 min at 56 degrees c or 24 h at ph 2: ndv-induced interferon is more stable under these conditions. hamsters inoculated with nwsm before and after infection with rabies virus are partially prot ...	1979	43690
hydrogen metabolism in aerobic hydrogen-oxidizing bacteria.	a survey on organisms able to use molecular hydrogen as electron donor in the energy-yielding process is presented. in the group of the aerobic hydrogen-oxidizing bacteria so far two types of hydrogenases have been encountered, a nad-reducing, soluble enzyme (h2 : nad oxidoreductase) and a membrane-bound enzyme unable to reduce pyridine nucleotides. with respect to the distribution of both types of hydrogenases three groups of hydrogen-oxidizing bacteria can be diffentiated containing (i) both t ...	1978	667183
[steroid metabolizing enzymes from microorganisms. vii. the nature of the biospecific binding to an affinity matrix-affinity chromatography of 4-en-3-oxo-steroid: (acceptor)-1-en-oxidoreductase from nocardia opaca].		1978	692123
[steroid converting enzymes from microorganisms. viii. effect of the organic solvent specific hydrophobicity of the binding on the affinity-matrix- affinity chromatography of a 4-en-3-oxo-steroid: (acceptor)-1-en-oxidoreductase from nocardia opaca].		1978	692134
[steroid transforming enzymes from microorganisms. v. purification of a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca and characterization of the prosthetic group].		1978	695702
[steroid conjugating enzyme from microorganisms. vi. purification of 4-en-o-oxosteroid: (acceptor)-1-en-oxidoreductase from nocardia opaca by affinity chromatography].		1977	916673
[steroid-transforming enzymes from microorganisms. ii. purification of a 3-oxosteroid: (2,6-dichlorophenolindophenol) delta 1-oxidoreductase from nocardia opaca by means of affinity chromatography].		1976	983139
a human t-independent b lymphocyte mitogen extracted from nocardia opaca.	nocardia water soluble mitogen (nwsm), a mitogen specific for mouse and rabbit b cells, activates blood, spleen, and tonsil lymphocytes from all the humans tested. evidence is presented that it acts selectively on human b lymphocytes. b-enriched cells were recovered from anti-ig-coated sephadex columns or after elimination of e rosette-forming cells: they were able to incorporate tritiated thymidine when they were cultivated in the presence of nwsm whereas they were not activated by pwm. convers ...	1976	1087313
free mycolic acids as criteria in the classification of nocardia and the 'rhodochrous' complex.	the methyl esters of free mycolic acids from representative strains of nocardia asteroides, n. brasiliensis, n. caviae and the 'rhodochrous' complex were subjected to detailed mass spectral analysis. the anhydromycolic esters of the nocardia strains consisted of homologous series containing from zero to three double bonds, with the main components of the parent mycolic acids centred on c52 to c54 (range c46 to c58). the anhydromycolates from one rhodochrous strain, nocardia opaca, had a molecula ...	1976	1107481
[breakdown of steroids. xv. formation of 9, 10-seco-compounds from 4-chlorosubstituted steroids in nocardia opaca].		1976	933521
presence and subcellular localization of two distinct mitogenic fractions in the cells of nocardia rubra and nocardia opaca: preparation of soluble mitogenic peptidoglycan fractions.	fractionation of cells of nocardia rubra and nocardia opaca led to the separation of the cell wall and a "cytoplasmic" fraction. both fractions were mitogenic for the splenocytes of akr and nude mice and of rabbits. the peptidoglycan was the active part of the cell wall fraction. the products solubilized by the action of streptomyces albus g peptidases on the peptidoglycan of n. rubra were mitogenic, but the products solubilized by lysoyme were not. tentative structures are proposed for these fr ...	1976	1278999
[degradation of steroids. xiv. synthesis of [1-14c]-succinic acid and [5-14c]-levulinic acid from 7a-methyl-5,6,7,7a-tetrahydroindan-[5-14c]-1,5-dion-4-(3-propionic acid-[1-14c]) by nocardia opaca].		1975	1052854
isolation of mitogenic and adjuvant active fractions from various species of nocardiae.	delipidated lysozyme digests of nocardia opaca, n. corallina, and n. rubra have been fractionated by sephadex filtration. the mitogenic and adjuvant activities of the fractions thus obtained have been investigated. all fractions are mitogenic except the last fraction of n.rubra, but the n. opaca products induce a stronger stimulation of mouse spleen lymphocytes than the corresponding fractions of the two other species. the activity of the first sephadex fractions of each strain has been compared ...	1975	1089598
[activity of microorganisms decomposing cholesterol].	the rates of cholesterol decomposition was compared among cell suspensions of different microorganisms. fifty seven cultures were studied: 2 strains of actinomycetes, 23 strains of proactinomycetes, 22 strains of mycobacteria, and 10 strains of bacteria. during four hours of incubation, 11 strains virtually did not decompose cholesterol at all, 10 strains decomposed it by up to 20 per cent, 21 strains by 20 to 70 per cent, and 15 strains by 70 to 100 per cent. the highest activity was displayed ...	1975	1226133
mitogenic effect of a water-soluble extract of nocardia opaca: a comparative study with some bacterial adjuvants on spleen and peripheral lymphocytes of four mammalian species.		1974	4207728
studies on a gram-positive hydrogen bacterium, nocardia opaca 1 b. iii. purification, stability and some properties of the soluble hydrogen dehydrogenase.		1974	4374147
nwsm, a mitogen from nocardia opaca, specific for rabbit bursa equivalent cells.		1974	4616010
distribution of the isopropylmalate pathway to leucine among diverse bacteria.	alpha-isopropylmalate synthase and beta-isopropylmalate dehydrogenase activities were detected in extracts of the following organisms: chromatium d, rhodopseudomonas spheroides, hydrogenomonas h16, pseudomonas aeruginosa, pseudomonas fluorescens, vibrio extorquens, rhizobium japonicum, alcaligenes viscolactis, escherichia coli b, proteus vulgaris, aerobacter aerogenes, salmonella typhimurium, micrococcus sp., micrococcus lysodeikticus, bacillus polymyxa, bacillus subtilis, and nocardia opaca. th ...	1974	4829932
studies on a gram-positive hydrogen bacterium, nocardia opaca strain 1 b. i. description and physiological characterization.		1973	4684599
studies on a gram-positive hydrogen bacterium, nocardia opaca strain 1 b. ii. enzyme formation and regulation under the influence of hydrogen or fructose as growth substrates.		1973	4684600
preparation and biological properties of water-soluble adjuvant fractions from delipidated cells of mycobacterium smegmatis and nocardia opaca.	digestion by lysozyme of delipidated cells of mycobacterium smegmatis liberates a water-soluble immunoadjuvant fraction which is chemically very similar to the water-soluble adjuvant (wsa) obtained previously from purified cell walls, but which contains somewhat more non-peptidoglycan amino acids. the yield of peptidoglycan-arabinogalactan complex is about 10 times greater starting from whole cells than from cell walls. the main biological properties of this "neo-wsa" are described: it increases ...	1973	4716544
[on the different types of nocardic acids isolated from nocardia opaca].		1966	4958541
utilization of anthranilic and nitrobenzoic acids by nocardia opaca and a flavobacterium.		1966	5912587
the metabolism of iron-, zinc- and manganese-deficient nocardia opaca.		1962	14005472
the effect of deficiency of iron, zinc and manganese on the growth and morphology of nocardia opaca.		1960	13843324
effect of substitution in the side-chain on beta-oxidation of aryloxy-alkylcarboxylic acids by nocardia opaca.		1959	13644179
production of pentose intermediates during growth of nocardia opaca and other saprophytic soil nocardias and mycobacteria.		1959	13818462
beta-oxidation of fatty acids by nocardia opaca.		1955	13278485
the morphology of nocardia opaca waksman & henrici (proactinomyces opacus jensen) when grown on hydrocarbons, vegetable oils, fatty acids and related substances.		1954	13221763
the metabolism of some saturated aliphatic hydrocarbons, alcohols and fatty acids by proactinomyces opacus jensen (nocardia opaca waksman & hendrik).		1952	12977738