Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| identification of candida strains isolated from tanzanian pregnant women with vaginal candidiasis. | to identify candida strains isolated from tanzanian women (13 to 45 years) with vaginal candidiasis. | 2005 | 16119751 |
| development of an integrative transformation system for the opportunistic pathogenic yeast candida lusitaniae using ura3 as a selection marker. | the nucleotide sequence of the ura3 gene encoding orotidine-5'-phosphate decarboxylase (omp dcase) of the human opportunistic pathogen yeast candida lusitaniae was determined by degenerate pcr and chromosome walking. deduced amino acid sequence showed strong homologies (59-85% identity) with omp dcases of different saccharomycetales and allowed identification of the known conserved domains. very close upstream from the ura3 gene, the 3'-end of a gene encoding a gea2-like protein was identified. ... | 2004 | 14755635 |
| susceptibility of clinical isolates of candida lusitaniae to five systemic antifungal agents. | the aim of the present study was to expand the mic database for candida lusitaniae in order to further determine its antifungal susceptibility pattern. | 2004 | 14963064 |
| delineation of clavispora lusitaniae clinical isolates by polymerase chain reaction-single strand conformation polymorphism analysis of the itsi region: a retrospective study comparing five typing methods. | strain delineation of the emerging opportunistic pathogen clavispora lusitaniae was studied using 12 strains, including two strains of known opposite mating type, cbs 6936 (h+) and cbs 5094 (h-), and 10 strains isolated between 1998 and 2001 from immunocompromized patients. this retrospective study assessed the occurrence of c. lusitaniae subtypes within and among hospitals, and in outpatients who were regularly screened for fungal infections in the course of radio-chemotherapy. strain typing wa ... | 2004 | 14982111 |
| significance of amplified fragment length polymorphism in identification and epidemiological examination of candida species colonization in children undergoing allogeneic stem cell transplantation. | candida albicans and non-c. albicans candida species are increasingly being isolated from patients in high-risk categories, most notably, those who have undergone stem cell transplantation (sct). identification of the presence of non-c. albicans candida species early in the course of the transplant procedure is important, as these species exhibit different sensitivities to the available antifungal treatments and cause mortality at rates that vary from those for c. albicans. amplified fragment le ... | 2004 | 15071024 |
| the predictors of outcome in immunocompetent patients with hematogenous candidiasis. | clinical parameters that predict outcome in non-immunosuppressed candidemic patients are not fully understood. | 2004 | 15109594 |
| in vitro pharmacodynamic characteristics of amphotericin b, caspofungin, fluconazole, and voriconazole against bloodstream isolates of infrequent candida species from patients with hematologic malignancies. | time-kill and postantifungal effect (pafe) of amphotericin b, caspofungin, fluconazole, and voriconazole were determined against clinical isolates of candida guilliermondii, candida kefyr, and candida lusitaniae. azoles displayed fungistatic activity and no measurable pafe, regardless of the concentration tested. amphotericin b and caspofungin demonstrated concentration-dependent fungicidal activity, although amphotericin b only produced a significant dose-dependent pafe against all isolates tes ... | 2004 | 15504881 |
| initial results from a longitudinal international surveillance programme for anidulafungin (2003). | this longitudinal study evaluated the in vitro activity of anidulafungin against 880 clinical yeast isolates and 68 mould isolates from 64 medical centres in north america, latin america and europe. | 2004 | 15564286 |
| [performance of the candida mannan antigen detection in patients with fungemia]. | for several years, the platelia candida mannan antigen enzyme immunoassay (candida eia) has been commercially available as a diagnostic test for invasive candidosis. we evaluated the candida eia with patients with proven fungemia caused by yeasts from which at least one serum sample was available. fifty-nine patients with 121 serum samples were included in the study. sixty-one different yeast strains were isolated from positive blood-cultures. the candida eia was positive (n = 35) or borderline ... | 2004 | 15667360 |
| candida lusitaniae peritonitis in a chronic ambulatory peritoneal dialysis patient. | this is a case report of a 69 year-old african american female with end-stage renal disease secondary to hypertensive nephrosclerosis on chronic ambulatory peritoneal dialysis (capd) for five years. her past was uneventful. she presented with symptoms and signs of acute peritonitis related to capd. there was initial response to antibiotics and culture was negative. the condition of the patient deteriorated despite the antibiotic. the peritoneal catheter was removed after five days of starting th ... | 2004 | 17642771 |
| candida lusitaniae infections in the era of fluconazole availability. | amphotericin b resistance among isolates of candida lusitaniae has distinguished it among candida species. because no comprehensive review has been published recently, we provide a case report and a literature review of c. lusitaniae infection to update and better characterize the illness in the era of azole availability and standardized methodologies for antifungal susceptibility testing. c. lusitaniae infection in the 55 cases surveyed in this review occurred in relatively young patients (medi ... | 2003 | 12522762 |
| comparison of three methods for in vitro susceptibility testing of candida species with flucytosine. | optimal methods for susceptibility testing of candida spp. with flucytosine have not been determined. breakpoints were recommended in 1984, but never validated. in this study, we compared the 1984 recommended macrodilution broth method (using an 80% endpoint) with a modification of the more recent nccls-recommended microdilution broth method with three endpoints-spectrophotometric 50% and 80% and a no growth endpoint determined by eye. nccls and british society for medical mycology (bsmm) breakp ... | 2003 | 12562694 |
| proficiency testing program for clinical laboratories performing antifungal susceptibility testing of pathogenic yeast species. | antifungal susceptibility testing is expected to facilitate the selection of adequate therapy for fungal infections. the general availability of antifungal susceptibility testing in clinical laboratories is low, even though a number of standard methods are now available. the objective of the present study was to develop and evaluate a proficiency testing program (ptp) for the antifungal susceptibility testing of pathogenic yeasts in laboratories licensed by the new york state department of healt ... | 2003 | 12624043 |
| flucytosine-fluconazole cross-resistance in purine-cytosine permease-deficient candida lusitaniae clinical isolates: indirect evidence of a fluconazole uptake transporter. | an unusual interaction between flucytosine and fluconazole was observed when a collection of 60 candida lusitaniae clinical isolates was screened for cross-resistance. among eight isolates resistant to flucytosine (mic >/= 128 micro g/ml) and susceptible to fluconazole (0.5 < mic < 2 micro g/ml), four became flucytosine-fluconazole cross resistant when both antifungals were used simultaneously. fluconazole resistance occurred only in the presence of high flucytosine concentrations, and the highe ... | 2003 | 12654658 |
| development and evaluation of the nuclisens basic kit nasba for the detection of rna from candida species frequently resistant to antifungal drugs. | we describe a nucleic acid sequence based amplification (nasba) protocol to detect 6 different candida species (candida krusei, candida glabrata, candida inconspicua, candida dubliniensis, candida norvegensis, candida lusitaniae) and compare it to a pcr assay. nasba showed a sensitivity of 1 colony forming unit and detected rna from all 6 candida species within 1 working day. all 5 patients with documented candidiasis showed identical results by both methods. this assay offers a sensitive, speci ... | 2003 | 12663165 |
| identification of yeast species from orange fruit and juice by rflp and sequence analysis of the 5.8s rrna gene and the two internal transcribed spacers. | yeast isolates from orange fruit and juice in a spontaneous fermentation were identified and classified by two molecular techniques. the first was analysis of the restriction pattern generated from the polymerase chain reaction (pcr)-amplified 5.8s rrna gene and the two internal transcribed spacers (its) using specific primers. the second technique was sequence analysis of the its regions using the same two primers. nine different restriction profiles were obtained from the size of the pcr produ ... | 2003 | 12702240 |
| existence of novel beta-1,2 linkage-containing side chain in the mannan of candida lusitaniae, antigenically related to candida albicans serotype a. | the antigenicity of candida lusitaniae cells was found to be the same as that of candida albicans serotype a cells, i.e. both cell wall mannans react with factors 1, 4, 5, and 6 sera of candida check. however, the structure of the mannan of c. lusitaniae was significantly different from that of c. albicans serotype a, and we found novel beta-1,2 linkages among the side-chain oligosaccharides, manbeta1-->2manbeta1--> 2manalpha1-->2manalpha1-->2man (lm5), and manbeta1-->2man-beta1-->2manbeta1-->2m ... | 2003 | 12787022 |
| genetic relatedness of candida strains isolated from women with vaginal candidiasis in malaysia. | the aims of this study were to compare the genetic relatedness of: (i) sequential and single isolates of candida strains from women with recurrent vaginal candidiasis (rvc); and (ii) candida strains from women who had only one episode of infection within a 1-year period. in total, 87 isolates from 71 patients were cultured, speciated and genotyped by random amplification of polymorphic dna (rapd) analysis. patients were categorized into three groups, namely those with: (i) a history of rvc from ... | 2003 | 12867559 |
| a monoclonal antibody directed against a candida albicans cell wall mannoprotein exerts three anti-c. albicans activities. | antibodies are believed to play a role in the protection against candida albicans infections by a number of mechanisms, including the inhibition of adhesion or germ tube formation, opsonization, neutralization of virulence-related enzymes, and direct candidacidal activity. although some of these biological activities have been demonstrated individually in monoclonal antibodies (mabs), it is not clear if all these anti-c. albicans activities can be displayed by a single antibody. in this report, ... | 2003 | 12933874 |
| disruption of ergosterol biosynthesis confers resistance to amphotericin b in candida lusitaniae. | candida lusitaniae is an emerging human pathogen that, unlike other fungal pathogens, frequently develops resistance to the commonly used antifungal agent amphotericin b. amphotericin b is a member of the polyene class of antifungal drugs, which impair fungal cell membrane integrity. here we analyzed mechanisms contributing to amphotericin b resistance in c. lusitaniae. sensitivity to polyenes in the related fungi saccharomyces cerevisiae and candida albicans requires the ergosterol biosynthetic ... | 2003 | 12936965 |
| colony morphology switching of candida lusitaniae and acquisition of multidrug resistance during treatment of a renal infection in a newborn: case report and review of the literature. | candida lusitaniae is an emerging opportunistic pathogen which exhibits an unusual antifungal susceptibility pattern. we describe a case of fatal renal infection due to c. lusitaniae in a very low birth weight neonate who was treated with short courses of fluconazole given alternately with amphotericin b. a colony morphology switching was detected on the standard primary culture medium by changes in colony size. switching was shown to affect deeply the susceptibility to amphotericin b. afterward ... | 2003 | 12967746 |
| clavispora (candida) lusitaniae susceptibility profiles and genetic diversity in three tertiary hospitals (1998-2001). | 2003 | 14522112 | |
| rapid identification of yeasts commonly found in positive blood cultures by amplification of the internal transcribed spacer regions 1 and 2. | a multiplex pcr method using one universal and eight species-specific primers was developed to rapidly identify eight yeast species found in positive blood cultures. the species-specific primers were designed from the internal transcribed spacer regions 1 and 2 of the rrna gene, whereas the universal primer was located at the 26s rrna gene. the eight species were candida albicans, candida glabrata, candida guilliermondii, candida krusei, candida lusitaniae, candida parapsilosis, candida tropical ... | 2003 | 14557922 |
| rapid and unequivocal differentiation of candida dubliniensis from other candida species using species-specific dna probes: comparison with phenotypic identification methods. | candida dubliniensis is a recently described opportunistic pathogen which shares many phenotypic characteristics with candida albicans but which has been reported to rapidly acquire resistance to azole antifungal drugs. therefore, differentiation of c. dubliniensis from c. albicans becomes important to better understand the clinical significance and epidemiologic role of c. dubliniensis in candidiasis. we compared phenotypic methods for the differentiation of c. dubliniensis from c. albicans (i. ... | 2003 | 14622344 |
| the d1/d2 domain of the large-subunit rdna of the yeast species clavispora lusitaniae is unusually polymorphic. | ten different versions of the d1/d2 divergent domain of the large-subunit ribosomal dna were identified among interbreeding members of the yeast species clavispora lusitaniae. one major polymorphism, located in a 90-bp structural motif of the d2 domain, exists in two versions that differ by 32 base substitutions. three other polymorphisms consist of a two-base substitution, a two-base deletion, and a single-base deletion, respectively. the polymorphisms are independent of one another and of the ... | 2003 | 14654429 |
| sterol and fatty acid composition of candida lusitaniae clinical isolates. | the sterol and fatty acid compositions of four amphotericin b-resistant and of two amphotericin b-susceptible candida lusitaniae clinical isolates were determined. a flow cytofluorometric susceptibility test (fcst) with a membrane potential-sensitive cationic dye was used as a complement to the conventional method for selecting the isolates. compared to susceptible isolates, resistant ones showed a greatly reduced ergosterol content and changes in sterol composition, consistent with a defect in ... | 2002 | 11796371 |
| rates and extents of antifungal activities of amphotericin b, flucytosine, fluconazole, and voriconazole against candida lusitaniae determined by microdilution, etest, and time-kill methods. | amphotericin b, flucytosine, fluconazole, and voriconazole alone and in combination were evaluated against isolates of candida lusitaniae. mics were determined by broth microdilution and etest, and time-kill studies were conducted. amphotericin b resulted in fungicidal activity against most isolates, whereas fluconazole, voriconazole, and flucytosine produced primarily fungistatic activities. the addition of flucytosine to amphotericin b resulted in a faster rate and greater extent of kill for i ... | 2002 | 11796383 |
| amphotericin b: spectrum and resistance. | amphotericin b is a polyene macrolide antibiotic derived from the actinomycete streptomyces nodosus. of the 200 known polyene agents, amphotericin b is the only one with toxicities that are sufficiently limited to permit intravenous administration. all polyenes have a common mechanism of action in that they preferentially bind to ergosterol, the primary sterol in the fungal cell membrane. the consequence of this binding includes disruption of the osmotic integrity of the membrane, with leakage o ... | 2002 | 11801575 |
| yeast species associated with orange juice: evaluation of different identification methods. | five different methods were used to identify yeast isolates from a variety of citrus juice sources. a total of 99 strains, including reference strains, were identified using a partial sequence of the 26s rrna gene, restriction pattern analysis of the internal transcribed spacer region (5.8s-its), classical methodology, the rapid yeast plus system, and api 20c aux. twenty-three different species were identified representing 11 different genera. distribution of the species was considerably differe ... | 2002 | 11916718 |
| change in colony morphology of candida lusitaniae in association with development of amphotericin b resistance. | it is not uncommon to see amphotericin b treatment failure in patients with systemic infection caused by candida lusitaniae. we report a patient with stage iv ovarian carcinoma and c. lusitaniae sepsis whose treatment with amphotericin b failed. the initial blood isolate was susceptible to amphotericin b in vitro; however, the mic for a blood isolate recovered 7 weeks after treatment began showed a fourfold increase. direct subculture of two positive blood samples obtained within a week of the p ... | 2002 | 11959563 |
| case report. candida lusitaniae peritonitis in a patient on continuous ambulatory peritoneal dialysis. | we report a case of candida lusitania peritonitis in continuous ambulatory peritoneal dialysis. since fluconazole therapy was not successful in this patient, the peritoneal catheter was removed and antifungal therapy continued, and the patient was then converted to haemodialysis. this treatment protocol was successful. we suggest that early peritoneal catheter removal should be considered in such cases. | 2002 | 12000515 |
| fatal disseminated candida lusitaniae infection in an infant with chronic granulomatous disease. | a 3-month-old boy born to a mother carrying an x-linked form of chronic granulomatous disease presented with persistent fever and hepatosplenomegaly. the diagnosis was confirmed as a gp91phox defect by genetic analysis, and the patient was managed with broad spectrum antibacterial agents, gamma-interferon and later amphotericin b. a liver biopsy revealed granulomata with budding yeast forms, and cultures of blood and urine grew candida lusitaniae. the patient died 26 days after admission. | 2002 | 12005097 |
| two cases of fungemia due to candida lusitaniae and a literature review. | reported here are two cases of candidemia caused by candida lusitaniae that occurred in two immunocompromised patients at hospital universitario "la fe" in valencia, spain. case 1 involved a low-birth-weight premature infant with congenital nephrotic syndrome who was successfully treated with amphotericin b, and case 2 involved a 50-year old woman with a high-grade malignancy lymphoma who succumbed to the infection. antifungal susceptibility testing of the candida lusitaniae isolates recovered f ... | 2002 | 12072941 |
| new chromogenic agar medium for the identification of candida spp. | a new chromogenic agar medium (candida diagnostic agar [cda]) for differentiation of candida spp. is described. this medium is based on sabouraud dextrose agar (oxoid cm41) and contains (per liter) 40.0 g of glucose, 10.0 g of mycological peptone, and 15.0 g of agar along with a novel chromogenic glucosaminidase substrate, ammonium 4-(2-[4-(2-acetamido-2-deoxy-beta-d-glucopyranosyloxy)-3-methoxyphenyl]-vinyl)-1-(propan-3-yl-oate)-quinolium bromide (0.32 g liter(-1)). the glucosaminidase substrat ... | 2002 | 12089051 |
| in vivo pathogenicity of eight medically relevant candida species in an animal model. | the relative pathogenicity of eight medically important candida species was investigated in a mouse model. | 2002 | 12382088 |
| identification and antifungal susceptibility of candida isolated from intensive care unit patients. | candida was isolated in 205 of 1060 clinical specimens (19.33%) in our laboratary sent from the intensive care unit for mycological investigation between january 98-december 99. all isolated strains were identified to species level using the api candida system (bio-meieux, france) as follows; candida albicans (n:115, 56.09%), candida tropicalis (n:23, 11.21%), candida parapsilosis (n:21, 10.24%), candida glabrata (n:12, 5.83%). candida kefyr (n:9, 4.39%), candida lusitaniae (n:7, 3.41%), candida ... | 2002 | 12437230 |
| candida lusitaniae as an unusual cause of recurrent vaginitis and its successful treatment with intravaginal boric acid. | increasing use of short-course antifungal therapies in patients with recurrent vulvovaginitis may enable the emergence of less-common, more resistant yeast strains as vaginal pathogens. we report the case of a patient with chronically symptomatic and repeatedly treated vaginal candidiasis whose infection was attributable to candida lusitaniae, a previously unreported cause of candidal vaginitis. | 2001 | 11916183 |
| improved detection of amphotericin b-resistant isolates of candida lusitaniae by etest. | both intrinsic and acquired resistance to amphotericin b have been documented for candida lusitaniae. amphotericin b remains the drug of choice for many critical fungal infections, and the detection of resistance is essential to monitor treatment effectively. the limitations of the national committee for clinical laboratory standards (nccls) reference methodology for detection of amphotericin b resistance are well documented, and several alternative methods have been proposed. etest assays with ... | 2001 | 11136795 |
| candida lusitaniae catheter-related sepsis. | to present a case describing candida lusitaniae candidemia in an immunocompetent patient successfully treated with fluconazole antifungal therapy. time-kill studies of the c. lusitaniae isolate using amphotericin b, and an extensive review of the literature are also presented. | 2001 | 11793623 |
| influence of glucose supplementation and inoculum size on growth kinetics and antifungal susceptibility testing of candida spp. | the influences of inoculum size and glucose supplementation on the growth kinetics of 60 candida spp. clinical isolates (candida albicans, candida tropicalis, candida parapsilosis, candida glabrata, candida krusei, and candida lusitaniae [10 isolates each]) are assessed. the combined influence of growth and reading method (visual or spectrophotometric) on the determination of the mics of amphotericin b, flucytosine, fluconazole, itraconazole, ketoconazole, and voriconazole is also analyzed, and ... | 2001 | 11158101 |
| candida lusitaniae: a cause of breakthrough fungemia in cancer patients. | candida lusitaniae is an infrequent cause of fungemia. we identified 12 cases of c. lusitaniae fungemia that occurred at the university of texas m. d. anderson cancer center from 1988 to 1999. the mean age of patients was 48 years (range 20--70 years). eight patients had hematologic malignancy or had received a bone marrow transplant, and 4 had a solid tumor. most patients (75%) were neutropenic (<10(3)/mm(3)). treatment with amphotericin b alone failed for 3 of 6 patients, irrespective of neutr ... | 2001 | 11170906 |
| fluconazole and voriconazole multidisk testing of candida species for disk test calibration and mic estimation. | fluconazole and voriconazole mics were determined for 114 clinical candida isolates, including isolates of candida albicans, candida glabrata, candida krusei, candida lusitaniae, candida parapsilosis, and candida tropicalis. all strains were susceptible to voriconazole, and most strains were also susceptible to fluconazole, with the exception of c. glabrata and c. krusei, the latter being fully fluconazole resistant. single-strain regression analysis (sra) was applied to 54 strains, including am ... | 2001 | 11283066 |
| secreted aspartic proteases of candida albicans, candida tropicalis, candida parapsilosis and candida lusitaniae. inhibition with peptidomimetic inhibitors. | the frequency of candida infections has increased in recent years and it has been accompanied by a significant rise in morbidity and mortality. the secretion of aspartic proteases by candida spp. was demonstrated to be one of the virulence determinants. candida albicans is classified as the major human pathogen in the genus candida. however, other species of this genus have been found to cause an increasing number of candidiases. we isolated secreted aspartic proteases (saps) of c. albicans (sap ... | 2001 | 11322888 |
| azasordarins: susceptibility of fluconazole-susceptible and fluconazole-resistant clinical isolates of candida spp. to gw 471558. | the in vitro activity of the azasordarin gw 471558 was compared with those of amphotericin b, flucytosine, itraconazole, and ketoconazole against 177 clinical isolates of candida spp. gw 471558 showed potent activity against candida albicans, candida glabrata, and candida tropicalis, even against isolates with decreased susceptibility to azoles. candida krusei, candida parapsilosis, candida lusitaniae, and candida guilliermondii are resistant to gw 471558 in vitro (mics, >128 microg/ml). | 2001 | 11353650 |
| systemic antifungal agents. | anti-fungal agents are classified under two major headings, systematic and topical agents. only systematic anti-fungal agents will be discussed in this chapter. since the discovery in 1955, amphotericin b has been the cornerstone of anti-fungal treatment. it is active against most species of fungi. however, candida lusitaniae, pseudallescheria boydii, and fusarium spp have primary resistance to amphotericin b. recently, new liposomal preparations of amphotericin b have been developed. they are l ... | 2001 | 11519290 |
| decaying wood in tree trunk hollows as a natural substrate for cryptococcus neoformans and other yeast-like fungi of clinical interest. | the occurrence of cryptococcus neoformans var. neoformans and other yeast-like fungi of clinical interest in decaying wood inside tree trunk hollows, bark and other plant materials is reported. the var. neoformans was isolated from 3 of 45 (6.6%) wood and one of 390 eucalyptus bark samples. two of the positive wood samples came from a tree trunk hollow of butea monosperma (family: papilionaceae) growing in roshan ara garden, old delhi whereas the third was from a trunk hollow of tamarindus indic ... | 2001 | 11554580 |
| alternative identification test relying upon sexual reproductive abilities of candida lusitaniae strains isolated from hospitalized patients. | the in vitro mating ability of candida lusitaniae (teleomorph clavispora lusitaniae) clinical isolates has been investigated. studying the effects of culture conditions, we showed that ammonium ion depletion in the medium is a major trigger of the sexual cycle. moreover, a solid support is required for mating, suggesting a role for adhesion factors in addition to the mating type gene recognition function. monitoring of mating and meiosis efficiency with auxotrophic strains showed great variation ... | 2001 | 11682506 |
| evaluation of etest method for determining caspofungin (mk-0991) susceptibilities of 726 clinical isolates of candida species. | the performance of the etest for testing the susceptibilities to caspofungin (mk-0991) of 726 isolates of candida spp. was assessed against the national committee for clinical laboratory standards (nccls) microdilution broth method. the nccls method employed rpmi 1640 broth medium, and mics were read after incubation for 48 h at 35 degrees c. mics were determined by etest for all 726 isolates with rpmi agar containing 2% glucose (rpg) and were read after incubation for 48 h at 35 degrees c. the ... | 2001 | 11724849 |
| [evaluation of a new chromogenic medium (candida id) for the isolation and presumptive identification of candida albicans and other medically important yeasts]. | candidiasis is a frequent human infection caused mainly by candida albicans. however, other species are emerging as important pathogens, as candida glabrata, candida parapsilosis, candida tropicalis, candida krusei or candida guilliermondii. rapid identification of clinical isolates could facilitate diagnosis and treatment. candida id (biomerieux, spain) is a new medium for the isolation and presumptive identification of yeasts: c. albicans grows as blue colonies, and c. tropicalis, c. guillierm ... | 2001 | 15482010 |
| [contamination of dairy products by yeasts-like fungi]. | evaluation of the degree of contamination of diary products with potentially pathogenic fungal strains. materials for the study were: yoghurt, cottage cheese, homogenised cottage cheese, cream and buttermilk. differentiation of species was based on their morphological features and biochemical properties (api 20 c biomérieux). among 300 studied diary products we have isolated 100 (33.0 +/- 2.72%) strains of yeast-like fungi. diary products were contamined with 19 species of fungi from types: cand ... | 2001 | 16894745 |
| a head-on comparison of the in vitro antifungal activity of conventional and lipid-based amphotericin b: a multicenter study. | a comparative study of conventional amphotericin b, abelcet and ambisome was performed using a microdilution format of the nccls m27-a methodology for susceptibility testing against 300 fungal isolates (152 yeasts, 148 filamentous fungi) in both rpmi-1640 and antibiotic medium #3 (ab3). the clinical isolates included candida albicans (n=54), candida glabrata (n=25), candida parapsilosis (n=23), candida krusei (n=19), candida lusitaniae (n=14), cryptococcus neoformans (n=5), candida tropicalis (n ... | 2000 | 10768512 |
| [evaluation of a new method for antifungal drugs susceptibility testing to yeasts]. | we compared the etest with a broth microdilution method (fp panel), performed according to the national committee for modified clinical laboratory standards (nccls) document m27-p guidelines, for determining the mics of 81 clinical isolates of yeasts (7 candida albicans, 8 candida glabrata, 10 candida parapsilosis, 6 pichia anomala, 10 candida tropicalis, 4 candida guilliermondii, 4 candida krusei, 6 trichosporon cutaneum, 5 candida ciferrii, 3 candida famata, 4 candida norvegensis, 2 rhodotorul ... | 2000 | 10783576 |
| a ste12 homolog is required for mating but dispensable for filamentation in candida lusitaniae. | candida lusitaniae is a dimorphic yeast that is emerging as an opportunistic fungal pathogen. in contrast to candida albicans, which is diploid and asexual, c. lusitaniae has been reported to have a sexual cycle. we have employed genetic approaches to demonstrate that c. lusitaniae is haploid and has a sexual cycle involving mating between mata and matalpha cells under nutrient deprivation conditions. by degenerate pcr, we identified a c. lusitaniae homolog (cls12) of the ste12 transcription fac ... | 2000 | 10790381 |
| comparative evaluation of a commercial system for identification of candida lusitaniae. | the ability of the api candida system (biomérieux, france) to identify candida lusitaniae isolates was evaluated in comparison to the auxacolor and id 32c systems using 52 clinical isolates previously identified on the basis of their morphology and their biochemical reactions in the auxacolor and id 32c systems. the api candida system failed to definitively identify most of the strains tested within 24 h. no beta-maltosidase activity was detected in 28 strains, and supplementary tests were requi ... | 2000 | 10898145 |
| flow cytometry antifungal susceptibility testing of pathogenic yeasts other than candida albicans and comparison with the nccls broth microdilution test. | candida species other than candida albicans frequently cause nosocomial infections in immunocompromised patients. some of these pathogens have either variable susceptibility patterns or intrinsic resistance against common azoles. the availability of a rapid and reproducible susceptibility-testing method is likely to help in the selection of an appropriate regimen for therapy. a flow cytometry (fc) method was used in the present study for susceptibility testing of candida glabrata, candida guilli ... | 2000 | 10991856 |
| nosocomial candidemia in non-neutropenic patients at an italian tertiary care hospital. | in a retrospective study conducted in an italian tertiary care hospital, the incidence of nosocomial candidemia was evaluated together with causative pathogens, treatment, and risk factors for death. over a 6-year period (1992-1997), a total of 189 episodes of candidemia occurred in 189 patients (mean age 58+/-19 years), accounting for an average incidence of 1.14 episodes per 10,000 patient-days per year. the most common reasons for hospitalization were solid neoplasia (21%), trauma (17%), abdo ... | 2000 | 11014622 |
| identification of rare candida species and other yeasts by polymerase chain reaction and slot blot hybridization. | invasive candidiasis has become a major cause of morbidity and mortality in immunocompromised hosts. here we describe a fast and reliable dna extraction and pcr amplification method in combination with a slot blot hybridization assay. a genus-specific probe was designed that allowed to detect dna from a broad range of candida species and 3 other yeasts. in addition, species-specific oligonucleotides for emerging candida and other yeast species allowed to identify dna extracted from candida lusit ... | 2000 | 11146245 |
| a phylogenetic study of ubiquinone q-8 species of the genera candida, pichia, and citeromyces based on 18s ribosomal dna sequence divergence. | to clarify phylogenetic relationships among species of the anamorphic ascomycetous genus candida with ubiquinone q-8, we determined complete sequences of 18s ribosomal rna genes (18s rdnas) from the type strains of 20 species of the genus candida and 7 of the teleomorphic ascomycetous genera pichia and citeromyces, which have q-8 as the major ubiquinone. q-8-forming candida species were divided into six clusters and were phylogenetically distant from a group of candida species that included the ... | 1999 | 12501366 |
| amphotericin b susceptibility testing of candida lusitaniae isolates by flow cytofluorometry: comparison with the etest and the nccls broth macrodilution method. | a flow cytofluorometric susceptibility test (fcst) was used for rapid determination of the susceptibility of candida lusitaniae isolates to amphotericin b. the test is based on the decrease in fluorescence intensity of cells stained with 3,3'-dipentyloxacarbocyanine iodide (dioc5(3)), a membrane potential-sensitive cationic dye, after drug treatment. a total of 58 c. lusitaniae clinical isolates including strains known to be amphotericin b-resistant on the basis of in-vivo and/or in-vitro data w ... | 1999 | 11252328 |
| in-vitro activity of five antifungal agents against uncommon clinical isolates of candida spp. | a broth microdilution method and an agar dilution method were used for testing fluconazole, itraconazole, ketoconazole, flucytosine and amphotericin b against 98 clinical isolates belonging to seven species of candida. the approximate rank order of fluconazole mics was candida lusitaniae approximately candida kefyr < candida famata approximately candida guilliermondii < candida pelliculosa approximately c. lipolytica approximately candida inconspicua. candida lypolitica and c. pelliculosa were t ... | 1999 | 11252339 |
| antifungal resistance in non- albicans candida species. | non- candida albicans species have emerged as important bloodstream pathogens. they tend to have decreased susceptibility to antifungal agents in vitro and cause infections associated with high morbidity and mortality. fluconazole resistance can emerge in any candida spp., but is most commonly seen with candida krusei, for which resistance is universal, and with candida glabrata. amphotericin b resistance has also been increasingly reported, most notably in isolates of candida lusitaniae and can ... | 1999 | 11504464 |
| invasive infections due to clavispora lusitaniae. | three cases of clavispora lusitaniae invasive fungal infections are reported. all three infections appeared in cancer patients presented with fungaemia, one additionally with meningitis. two of them were breakthrough -- they developed during therapy with conventional amphotericin b with a dose of 0.5 mg kg(-1) day(-1) . all three were cured: two with intravenous fluconazol and one with an increasing dose (1 mg kg(-1) day(-1)) of amphotericin b. in one of two breakthrough cases the sensitivity of ... | 1999 | 10030550 |
| high-frequency, in vitro reversible switching of candida lusitaniae clinical isolates from amphotericin b susceptibility to resistance. | recent studies have revealed an increase in the incidence of serious infections caused by non-albicans candida species. candida lusitaniae is of special interest because of its sporadic resistance to amphotericin b (amb). the present in vitro study demonstrated that, unlike other candida species, c. lusitaniae isolates frequently generated amb-resistant lineages form previously susceptible colonies. cells switching from a resistant colony to a susceptible phenotype were also detected after treat ... | 1999 | 10103188 |
| invasive yeast infections other than candida spp. in acute leukaemia. | during the last two decades, the treatment of leukaemia has changed significantly; increasing intensity of chemotherapy and bone marrow transplantation have lead to profound immunosuppression, prolonged stays in hospital, vascular catheterization, administration of broad spectrum antimicrobial agents and extensive use of prophylactic antifungal drugs. all but the last of these risk factors have increased the incidence of fungal infections in leukaemic patients and have significantly changed the ... | 1999 | 10204120 |
| in-vitro activity of dicationic aromatic compounds and fluconazole against cryptococcus neoformans and candida spp. | we investigated the in-vitro activity of three selected dicationic aromatic compounds for nine clinical isolates of cryptococcus neoformans and 93 clinical isolates of candida spp., representing 12 different species, using a broth macrodilution method following nccls recommendations. all the clinical isolates were also tested for fluconazole susceptibility. the in-vitro data demonstrate that compounds 39 and 57 have excellent in-vitro activity for all tested strains (mic 0.19-1.56 mg/l) except c ... | 1999 | 10473229 |
| comparative in vitro activity of antiseptics and disinfectants versus clinical isolates of candida species. | to evaluate the in vitro activity of antiseptics and detergents against candida. | 1999 | 10530645 |
| species and genotypic diversities and similarities of pathogenic yeasts colonizing women. | we examined the patterns of strain relatedness among pathogenic yeasts from within and among groups of women to determine whether there were significant associations between genotype and host condition or body site. a total of 80 yeast strains were isolated, identified, and genotyped from 49 female volunteers, who were placed in three groups: (i) 19 women with aids, (ii) 11 pregnant women without human immunodeficiency virus (hiv) infection, and (iii) 19 women who were neither pregnant nor infec ... | 1999 | 10565893 |
| fungal peritonitis in patients on continuous ambulatory peritoneal dialysis. | the purpose of this study was to analyze the microbiological and clinical features of fungal peritonitis in patients with endstage renal failure treated with continuous ambulatory peritoneal dialysis (capd). the diagnosis of peritonitis was based on abdominal discomfort or pain, cloudy peritoneal effluent with an elevated leukocyte count and isolation of fungi from the peritoneal effluent. amphotericin b, flucytosine, ketoconazole, miconazole and more recently fluconazole were used for antifunga ... | 1998 | 10052546 |
| prospective study of nosocomial fungal meningitis in children--report of 10 cases. | within an 8-year period, 10 cases of fungal nosocomial meningitis in children 0-13 y old were prospectively identified, 3 caused by yeasts other than candida spp. (rhodotorula rubra, aureobasidium mansoni, clavispora lusitaniae) and 7 by candida albicans. seven patients survived. whereas 3 neonates with fungal meningitis (all due to c. albicans) died. risk factors for fungal nosocomial meningitis included cancer (2 children), previous neurosurgery (2 children), cranial trauma (1 case) and premat ... | 1998 | 10066050 |
| genetic characterization of a phospholipase c gene from candida albicans: presence of homologous sequences in candida species other than candida albicans. | phospholipase c (plc) enzymes are essential in regulating several important cellular functions in eukaryotes, including yeasts. in this study, pcr was used to identify a gene encoding plc activity in candida albicans, using oligonucleotide primers complementary to sequences encoding highly conserved amino acid regions within the x domains of previously characterized eukaryotic phospholipase c genes. the nucleotide sequence of the c. albicans gene, caplc1 (2997 bp), was determined from a recombin ... | 1998 | 9467900 |
| olecranon bursitis caused by infection with candida lusitaniae. | we describe a 59-year-old woman with diabetes and chronic asthma treated with prednisone and methotrexate who developed chronic olecranon bursitis caused by candida lusitaniae. infection, especially with unusual microbial pathogens, should be considered in cases of chronic bursitis in patients taking immunosuppressive medicine, even if the classic signs of septic bursitis are absent. infection with c. lusitaniae, a component of the normal mycoflora, may be a marker of serious immunosuppression, ... | 1998 | 9517788 |
| candida lusitaniae: an uncommon cause of prosthetic valve endocarditis. | 1998 | 9524867 | |
| nosocomial breakthrough fungaemia during antifungal prophylaxis or empirical antifungal therapy in 41 cancer patients receiving antineoplastic chemotherapy: analysis of aetiology risk factors and outcome. | forty-one episodes of breakthrough fungaemia occurring over a 7.5 year period in the national and st elizabeth's cancer institutes in bratislava, slovakia, were analysed. five of them occurred during prophylaxis with fluconazole (one torulopsis glabrata, one hansenula anomala, two candida krusei and one candida parapsilosis), ten with itraconazole (three trichosporon pullulans, one trichosporon beigelii, one cryptococcus laurentii, three candida albicans and two t. glabrata), 11 during prophylax ... | 1998 | 9578164 |
| in vitro activities of terbinafine against cutaneous isolates of candida albicans and other pathogenic yeasts. | terbinafine is active in vitro against a wide range of pathogenic fungi, including dermatophytes, molds, dimorphic fungi, and some yeasts, but earlier studies indicated that the drug had little activity against candida albicans. in contrast, clinical studies have shown topical and oral terbinafine to be active in cutaneous candidiasis and candida nail infections. in order to define the anti-candida activity of terbinafine, we tested the drug against 350 fresh clinical isolates and additional str ... | 1998 | 9593126 |
| evidence for person-to-person transmission of candida lusitaniae in a neonatal intensive-care unit. | candida lusitaniae is an increasingly important nosocomial bloodstream pathogen. epidemiological investigation and molecular typing techniques identified three neonates infected with identical strains of c lusitaniae that were distinguished readily from epidemiologically unrelated strains from other locations in the hospital. the results of this study provide evidence for nosocomial transmission of c lusitaniae in a neonatal intensive-care unit and suggest that these infants are at increased ris ... | 1998 | 9613696 |
| evaluation of the etest method for determining fluconazole susceptibilities of 402 clinical yeast isolates by using three different agar media. | the performance of the etest for fluconazole susceptibility testing of 402 yeast isolates was assessed against the national committee for clinical laboratory standards (nccls) microdilution broth method. the nccls method employed rpmi 1640 broth medium, and mics were read after incubation for 48 h at 35 degrees c. etest mics were determined with rpmi agar containing 2% glucose (rpg), casitone agar (cas), and mueller-hinton agar (mha) and were read after incubation for 48 h at 35 degrees c. the y ... | 1998 | 9705397 |
| fungemia in cancer patients in brazil: predominance of non-albicans species. | the objective of this study was to characterize the epidemiology of candidemia in cancer patients in the city of rio de janeiro, brazil. an 18-month survey of fungemia in patients with cancer was undertaken in three hospitals in rio de janeiro. forty-three episodes of candidemia were identified in 43 patients, 43 of which were episodes of candidemia; in ten case the strains were not available for further identification of species and were excluded from this analysis. the overall distribution of ... | 1998 | 9750336 |
| first isolation of trichophyton fischeri in the united states. | a 49-year-old male with pneumocystis carinii pneumonia was seen at bellevue hospital in new york, n.y. sputum samples yielded cultures of candida lusitaniae, mycobacterium avium, and a filamentous fungus, trichophyton fischeri. t. fischeri is a nonpathogenic fungus which resembles the dermatophyte trichophyton rubrum. this is the first record of the species from u.s. sources. this case exemplifies the ecological differences between t. fischeri and t. rubrum and illustrates how correct identifica ... | 1998 | 9774603 |
| comparison of a new commercial colorimetric microdilution method with a standard method for in-vitro susceptibility testing of candida spp. and cryptococcus neoformans. | the sensititre yeast one method (accumed international ltd, east grinstead, uk) is a microplate-based procedure that incorporates an oxidation-reduction indicator, alamar blue, for the in-vitro testing of five antifungal agents (amphotericin b, fluconazole, itraconazole, ketoconazole and flucytosine). we compared this colorimetric method with a standard broth microdilution test, performed according to us national committee for clinical laboratory standards document m27-a guidelines, for determin ... | 1998 | 9818741 |
| amphotericin b-metronidazole combination against candida spp. | interaction between amphotericin b and metronidazole was studied against candida albicans, candida tropicalis, candida parapsilosis, candida krusei, and candida lusitaniae strains. minimum inhibitory concentrations (mics) of the drugs alone and in combination were determined by means of the checkerboard method on ynb supplemented agar. minimum fungicidal concentrations (mfcs) were determined on sabouraud dextrose agar. based on the mic and mfc values, fractionary indices were determined respecti ... | 1998 | 17655414 |
| in-vitro susceptibility pattern of candida lusitaniae and evaluation of the etest method. | the antifungal susceptibility of 35 candida lusitaniae isolates was determined in vitro by the national committee for clinical laboratory standards (nccls) m27-p macrodilution methodology. all the isolates were susceptible to ketoconazole, itraconazole and fluconazole. of the 35 isolates, eight (23%) were resistant to flucytosine. for amphotericin b, m27-p yielded a narrow range of mics (0.06-0.5 mg/l). we therefore investigated the activity of this drug by reading mics at 72 h and by using alte ... | 1997 | 9184357 |
| in vitro antifungal activity of bms-181184 against systemic isolates of candida, cryptococcus, and blastomyces species. | bms-181184 is a water-soluble derivative of the pradimicin group of antifungal compounds. we determined the in vitro activities of bms-181184 and comparator agents amphotericin b, 5-fluorocytosine, fluconazole, and ketoconazole against 184 systemic fungal isolates collected at the health sciences centre in winnipeg, canada, between 1987 and 1995. bms-181184 demonstrated mics of between 1 and 8 micrograms/ml for all candida albicans, candida glabrata, candida tropicalis, candida krusei, candida l ... | 1997 | 9327245 |
| evaluation of the echinocandin antifungal mk-0991 (l-743,872): efficacies in mouse models of disseminated aspergillosis, candidiasis, and cryptococcosis. | the in vivo activity of the merck antifungal echinocandin drug candidate mk-0991 (l-743,872) was evaluated in mouse models of disseminated candidiasis, aspergillosis, and cryptococcosis. the echinocandins are potent inhibitors of 1,3-beta-d-glucan synthase. two models of disseminated candidiasis were used. in a candida albicans mouse survival model with both dba/2n and cd-1 mice, estimates of the 50% effective doses (ed50s) of mk-0991 were 0.04 and 0.10 mg/kg of body weight/dose at 21 days after ... | 1997 | 9371329 |
| activity of a new triazole, sch 56592, compared with those of four other antifungal agents tested against clinical isolates of candida spp. and saccharomyces cerevisiae. | sch 56592 is a new triazole agent with potent, broad-spectrum antifungal activity. the in vitro activities of sch 56592, itraconazole, fluconazole, amphotericin b, and flucytosine (5-fc) against 404 clinical isolates of candida spp. (382 isolates) and saccharomyces cerevisiae (22 isolates) were investigated. in vitro susceptibility testing was performed by a broth microdilution method performed according to national committee for clinical laboratory standards guidelines. overall, sch 56592 was v ... | 1997 | 9021172 |
| influence of l-galactonic acid gamma-lactone on ascorbate production in some yeasts. | l-galactonic acid gamma-lactone appear to influence ascorbic and production in strains of saccharomyces cerevisiae, clavispora lusitaniae, cryptococcus terreus, pichia fermentans in which this is undetected whenever glucose represents the sole carbon source. cryptococcus terreus (strains dbvp 6012 and 6242) does not show ascorbic acid production either in presence or in the absence of l-galactonic acid gamma-lactone. this feature is probably connected to the insensibility of the strain to the ly ... | 1997 | 9111923 |
| candida lusitaniae chorioamnionitis in a bone marrow transplant patient. | 1997 | 11770607 | |
| efficacy of continuous flucytosine infusion against candida lusitaniae in experimental hematogenous murine candidiasis. | candida lusitaniae may cause life-threatening infections in the immunocompromised host and may be resistant to amphotericin b. flucytosine (5-fc) is very active against c. lusitaniae isolates in vitro, while the in vivo response of murine infection to 5-fc is not as good. to evaluate the hypothesis that this discrepancy may be primarily due to the short half-life of 5-fc in mice, we compared the same total dosage of 75 mg of 5-fc per kg of body weight per day given by bolus injections or infused ... | 1996 | 9124867 |
| evaluation of a commercial medium for identification of candida species. | chromagar candida (chromagar, france) was evaluated as a medium for the presumptive identification and isolation of yeasts using 1,537 isolates of medically important yeasts, including 970 candida albicans, 165 candida parapsilosis, 131 candida glabrata, 62 candida guilliermondii, 35 candida krusei, 32 candida tropicalis, 31 rhodotorula rubra, 23 trichosporon spp. (17 trichosporon beigelii), 17 candida famata, 16 candida pelliculosa, 10 pichia etchelsii, 10 saccharomyces cerevisiae, 8 candida lu ... | 1996 | 8801088 |
| comparison of isolator 1.5 and bactec nr660 aerobic 6a blood culture systems for detection of fungemia in children. | the isolator 1.5 microbial system (iso 1.5) (wampole laboratories, cranbury, n.j.) was compared with the bactec nr660 aerobic nr6a bottle (nr6a) (becton dickinson diagnostic instrument systems, sparks, md.) for the detection of fungemia in hospitalized pediatric patients. for 4,825 paired blood cultures evaluated retrospectively from april 1992 to december 1994, at least one blood culture system was positive for 89 clinically important fungal isolates involved in 36 episodes of fungemia in 34 pa ... | 1996 | 8818873 |
| antifungal susceptibility testing of yeasts: evaluation of technical variables for test automation. | the technical parameters for antifungal susceptibility testing with candida species were reexamined to determine the optimal conditions for testing with semiautomated preparations of broth microdilution cultures, automated spectrophotometric readings of the cultures, and dose-response and endpoint determinations by means of a computer spreadsheet. tests were based on proposed standard method m27p of the national committee for clinical laboratory standards for antifungal agents. rpmi 1640 broth w ... | 1995 | 8540715 |
| yeast communities in a natural tequila fermentation. | fresh and cooked agave, drosophila spp., processing equipment, agave molasses, agave extract, and fermenting must at a traditional tequila distillery (herradura, amatitan, jalisco, méxico) were studied to gain insight on the origin of yeasts involved in a natural tequila fermentations. five yeast communities were identified. (1) fresh agave contained a diverse mycobiota dominated by clavispora lusitaniae and an endemic species, metschnikowia agaveae. (2) drosophila spp. from around or inside the ... | 1995 | 8546452 |
| comparative evaluation of macrodilution and alamar colorimetric microdilution broth methods for antifungal susceptibility testing of yeast isolates. | a comparative evaluation of the macrodilution method and the alamar colorimetric method for the susceptibility testing of amphotericin b, fluconazole, and flucytosine was conducted with 134 pathogenic yeasts. the clinical isolates included 28 candida albicans, 17 candida tropicalis, 15 candida parapsilosis, 12 candida krusei, 10 candida lusitaniae, 9 candida guilliermondii, 18 torulopsis glabrata, and 25 cryptococcus neoformans isolates. the macrodilution method was performed and interpreted acc ... | 1995 | 8567901 |
| molecular probes for diagnosis of fungal infections. | we have developed 21 specific nucleic acid probes which target the large subunit rrna genes from aspergillus flavus, aspergillus fumigatus, aspergillus glaucus, aspergillus niger, aspergillus terreus, blastomyces dermatitidis, candida albicans, candida (torulopsis) glabrata, candida guilliermondii, candida kefyr, candida krusei, candida lusitaniae, candida parapsilosis, candida tropicalis, coccidioides immitis, cryptococcus neoformans var. gattii, cryptococcus neoformans var. neoformans, filobas ... | 1995 | 8576345 |
| use of chromagar candida medium for isolation of yeasts from dental samples. | a new differential medium, chromagar candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. the recovery of yeasts on the medium was not significantly different from the recovery on sabouraud dextrose agar. the identities of 450 green colonies on chromagar candida, presumptively identified as candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-n-acetylgalacto ... | 1995 | 8576366 |
| new and emerging yeast pathogens. | the most common yeast species that act as agents of human disease are candida albicans, candida tropicalis, candida glabrata, candida parapsilosis, and cryptococcus neoformans. the incidence of infections by other yeasts has increased during the past decade. the most evident emerging pathogens are malassezia furfur, trichosporon beigelii, rhodotorula species, hansenula anomala, candida lusitaniae, and candida krusei. organisms once considered environmental contaminants or only industrially impor ... | 1995 | 8665465 |
| comparison of four dna-based methods for strain delineation of candida lusitaniae. | four methods for the accurate delineation of epidemiologically related and unrelated strains of candida lusitaniae were compared. three pulsed-field electrophoretic methods, including two contour-clamped homogeneous field gel electrophoresis methods (ekp-1 and ekp-2) yielding electrophoretic karyotype patterns of intact chromosomal dna and a method in which the chromosomal dna was macrodigested with the endonuclease sfii prior to pulsed-field electrophoresis (mdp), and a random amplified polymor ... | 1995 | 7650168 |
| activities of d0870, a novel triazole, against candida lusitaniae and trichosporon beigelii in experimental murine infections. | candida lusitaniae and trichosporon beigelii may cause life-threatening infections in the immunocompromised host and may be resistant to amphotericin b. we assessed the activities of a new triazole, d0870, against one t. beigelii and four c. lusitaniae strains, in comparison with those of fluconazole and amphotericin b. immunosuppressed cf1 mice, intravenously infected with each fungal strain, received 3 days of therapy with oral d0870 (5 or 25 mg/kg of body weight daily), fluconazole (5 to 50 m ... | 1995 | 7726538 |
| epithelial adhesion in yeast species: correlation with surface expression of the integrin analog. | epithelial adhesion and expression of the integrin analog, a putative candidal adhesion, were correlated for 33 clinical and laboratory isolates of candida albicans, other candida species, and saccharomyces cerevisiae. on flow cytometry with saturating concentrations of the monoclonal antibody okm1, surface fluorescence was highest for c. albicans at 67.8% +/- 1.7% and significantly reduced for candida tropicalis (32.0% +/- 2.6%), candida parapsilosis (18.3% +/- 2.4%), candida glabrata (3.3% +/- ... | 1995 | 7769314 |
| comparative study of broth macrodilution and microdilution techniques for in vitro antifungal susceptibility testing of yeasts by using the national committee for clinical laboratory standards' proposed standard. | a comparative study of broth macro- and microdilution methods for susceptibility testing of fluconazole, itraconazole, flucytosine, and amphotericin b was conducted with 273 yeasts. the clinical isolates included 100 candida albicans, 28 candida tropicalis, 25 candida parapsilosis, 15 candida lusitaniae, 15 candida krusei, 50 cryptococcus neoformans var. neoformans, 25 torulopsis (candida) glabrata, and 15 trichosporon beigelii strains. both methods were performed according to the national commi ... | 1994 | 7814488 |
| efficacy of escalating doses of liposomal amphotericin b (ambisome) against hematogenous candida lusitaniae and candida krusei infection in neutropenic mice. | immunosuppressed cf1 mice were infected intravenously with two strains of candida krusei and four strains of candida lusitaniae (two of which were resistant to amphotericin b). mice were treated with 1 or 2 mg of amphotericin b desoxycholate per kg of body weight per day or escalating doses of liposomal amphotericin b (8 to 30 mg/kg/day) or were left untreated. higher doses of liposomal amphotericin b were as effective as standard dose of amphotericin b desoxycholate in prolonging survival but w ... | 1994 | 7872764 |