Publications

mycobacteria clumping increase their capacity to damage macrophages.	the rough morphotypes of non-tuberculous mycobacteria have been associated with the most severe illnesses in humans. this idea is consistent with the fact that mycobacterium tuberculosis presents a stable rough morphotype. unlike smooth morphotypes, the bacilli of rough morphotypes grow close together, leaving no spaces among them and forming large aggregates (clumps). currently, the initial interaction of macrophages with clumps remains unclear. thus, we infected j774 macrophages with bacterial ...	2016	27757105
clinical efficacy and safety of multidrug therapy including thrice weekly intravenous amikacin administration for mycobacterium abscessus pulmonary disease in outpatient settings: a case series.	mycobacterium abscessus (m. abscessus) pulmonary disease is a refractory chronic infectious disease. options for treating m. abscessus pulmonary disease are limited, especially in outpatient settings. among parenteral antibiotics against m. abscessus, intravenous amikacin (amk) is expected to be an effective outpatient antimicrobial therapy. this study evaluated the clinical efficacy and safety of intravenous amk therapy in outpatients with m. abscessus pulmonary disease.	2016	27506679
mab_3551c encodes the primary triacylglycerol synthase involved in lipid accumulation in mycobacterium abscessus.	slow growing pathogenic mycobacteria utilize host-derived lipids and accumulate large amounts of triacylglycerol (tag) in the form of intracytoplasmic lipid inclusions (ili), serving as a source of carbon and energy during prolonged infection. mycobacterium abscessus is an emerging and rapidly growing species capable to induce severe and chronic pulmonary infections. however, whether m. abscessus, like mycobacterium tuberculosis, possesses the machinery to acquire and store host lipids, remains ...	2016	27513974
mycobacterium abscessus-induced granuloma formation is strictly dependent on tnf signaling and neutrophil trafficking.	mycobacterium abscessus is considered the most common respiratory pathogen among the rapidly growing non-tuberculous mycobacteria. infections with m. abscessus are increasingly found in patients with chronic lung diseases, especially cystic fibrosis, and are often refractory to antibiotic therapy. m. abscessus has two morphotypes with distinct effects on host cells and biological responses. the smooth (s) variant is recognized as the initial airway colonizer while the rough (r) is known to be a ...	2016	27806130
clofazimine enteropathy: a rare and underrecognized complication of mycobacterial therapy.	clofazimine-induced crystal-storing histiocytosis is a rare complication of treatment previously reported in dermatology literature as a complication of leprosy therapy. we report a case of disseminated mycobacterium abscessus requiring treatment with high-dose oral clofazimine resulting in enteropathy in a patient who presented with abdominal pain, malnutrition, and melena.	2016	27800519
cutaneous mycobacterium abscessus infection following hair transplant.	a 28-year-old man presented with a 10-day history of scalp nodules. he had undergone hair transplantation 2 months previously. incision and drainage of one of the nodules yielded gelatinous material, which was sent for microscopy and culture, including low-temperature culture. after 2 weeks of incubation, culture of the nodule yielded acid- and alcohol-fast bacilli, which were identified as mycobacterium abscessus, a rapidly growing, nontuberculous mycobacterium, which has been reported to cause ...	2016	27663154
complete genome sequences of 17 rapidly growing nontuberculous mycobacterial strains.	we report the complete genome sequences of 17 rapidly growing nontuberculous mycobacterial (ntm) strains, including 16 mycobacterium abscessus complex strains and one m. immunogenum strain. these sequences add value to studies of the genetic diversity of rapidly growing ntm strains recovered from human specimens.	2016	27660787
survival of mycobacterium abscessus isolated from people with cystic fibrosis in artificially generated aerosols.		2016	27660513
non-tuberculous mycobacteria: classification, diagnostics, and therapy.	non-tuberculous mycobacteria (ntm) are species other than those belonging to the mycobacterium tuberculosis complex and do not cause leprosy. ntm are generally free-living organisms that are ubiquitous in the environment. there have been more than 140 ntm species identified to-date. they can cause a wide range of infections, with pulmonary infections being the most frequent (65-90 %). there is growing evidence that the incidence of ntm lung diseases and associated hospitalizations are on the ris ...	2016	27645543
mgtc as a host-induced factor and vaccine candidate against mycobacterium abscessus infection.	mycobacterium abscessus is an emerging pathogenic mycobacterium involved in pulmonary and mucocutaneous infections, presenting a serious threat for patients with cystic fibrosis (cf). the lack of an efficient treatment regimen and the emergence of multidrug resistance in clinical isolates require the development of new therapeutic strategies against this pathogen. reverse genetics has revealed genes that are present in m. abscessus but absent from saprophytic mycobacteria and that are potentiall ...	2016	27481243
identification of antimicrobial activity among new sulfonamide metal complexes for combating rapidly growing mycobacteria.	mycobacteriosis is a type of infection caused by rapidly growing mycobacteria (rgm), which can vary from localized illness, such as skin disease, to disseminated disease. amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem and sulfamethoxazole are antimicrobial drugs chosen to treat such illnesses; however, not all patients obtain the cure. the reason why the treatment does not work for those patients is related to the fact that some clinical strains present resistance to t ...	2016	27567903
antibiofilm effect of antimicrobials used in the therapy of mycobacteriosis.	rapidly growing mycobacteria (rgm) are opportunistic pathogens found in the environment. when in biofilms, mycobacteria is highly resistant to antibacterial treatments. the purpose of this study is to evaluate the antibiofilm activity of antimicrobials commonly used in therapy against mycobacteria. the antimicrobial susceptibility of mycobacterium abscessus, mycobacterium fortuitum and mycobacterium massiliense was determined in planktonic and sessile populations. the antimicrobials amikacin, ci ...	2016	27554273
mycobacterium abscessus scleritis after a baerveldt glaucoma implant.		2016	27552708
[mycobacterium abscessus pulmonary disease: important pathogen involved in microbial substitution during the treatment of non-abscessus mycobacterial disease].	mycobacterium abscessus pulmonary disease is common in patients with bronchiectasis. however, the underlying disease that is more likely to be present in patients with m. abscessus pulmonary disease remains poorly understood.	2016	27530020
peak plasma concentration of azithromycin and treatment responses in mycobacterium avium complex lung disease.	macrolides, such as azithromycin (azm) and clarithromycin, are the cornerstones of treatment for mycobacterium avium complex lung disease (mac-ld). current guidelines recommend daily therapy with azm for cavitary mac-ld and intermittent therapy for noncavitary mac-ld, but the effectiveness of these regimens has not been thoroughly investigated. this study evaluated associations between microbiological response and estimated peak plasma concentrations (cmax) of azm. the azm cmax was measured in p ...	2016	27480854
cystic teratoma mimicking recurrent pleural effusion, complicated by mycobacterium abscessus infection.	teratomas of anterior mediastinum are rare. they are often slow growing, asymptomatic, and detected incidentally on chest imaging. mycobacterium abscessus (m. abscessus) is an acid-fast bacillus that is classified as a pathogenic "rapid growing" non-tuberculous mycobacteria. it is an uncommon cause of human pathology, which may cause skin and soft tissue infection after skin injury following inoculation, minor trauma, and surgery. here, we present an unusual case of benign cystic teratoma mimick ...	2016	27516884
disseminated talaromyces marneffei and mycobacterium abscessus in a patient with anti-interferon-γ autoantibodies.	anti-interferon (ifn)-γ autoantibodies are increasingly recognized as a cause of adult-onset immunodeficiency and increased risk for infections with intracellular pathogens. we report on disseminated talaromyces (penicillium) marneffei and mycobacterium abscessus infection in a 72-year-old, human immunodeficiency virus noninfected, thai man with anti-ifn-γ autoantibody. the patient was successfully treated with antimicrobial therapy and rituximab to control b cell-derived autoantibodies.	2016	27419165
diagnosis and treatment of nontuberculous mycobacterial lung disease.	nontuberculous mycobacteria (ntm) are ubiquitous organisms; their isolation from clinical specimens does not always indicate clinical disease. the incidence of ntm lung diseases has been increasing worldwide. although the geographic diversity of ntm species is well known, mycobacterium avium complex (mac), m. abscessus complex (mabc), and m. kansasii are the most commonly encountered and important etiologic organisms. two distinct types of ntm lung diseases have been reported, namely fibrocavita ...	2016	27134484
deletion of a dehydratase important for intracellular growth and cording renders rough mycobacterium abscessus avirulent.	mycobacterium abscessus (mabs) is a rapidly growing mycobacterium and an emerging pathogen in humans. transitioning from a smooth (s) high-glycopeptidolipid (gpl) producer to a rough (r) low-gpl producer is associated with increased virulence in zebrafish, which involves the formation of massive serpentine cords, abscesses, and rapid larval death. generating a cord-deficient mabs mutant would allow us to address the contribution of cording in the physiopathological signs of the r variant. herein ...	2016	27385830
the impact of protein extraction protocols on the performance of currently available maldi-tof mass spectrometry for identification of mycobacterial clinical isolates cultured in liquid media.	protein extraction step is particularly important for identification of mycobacterial isolates by maldi-tof mass spectrometry (ms) because of its thick and solid cell wall. this study compared the performance of maldi-tof ms for identification of mycobacterial clinical isolates cultured in liquid media between heating-based protocol and non-heating protocol.	2016	27380997
disseminated mycobacterium abscessus complex infection manifesting as multiple areas of lymphadenitis and skin abscess in the preclinical stage of acute lymphocytic leukemia.	a 37-year-old woman was admitted to a hospital due to a prolonged fever and a rash on her legs. she had systemic lymphadenitis and a skin abscess on her left leg. pathological findings of a left leg skin biopsy revealed abscess formation with granulomatous dermatitis, mycobacterium abscessus complex was cultured from the resected left supraclavicular lymph node, and disseminated m. abscessus complex infection was diagnosed. she was treated with combination treatment with antimicrobials and percu ...	2016	27374685
trehalose polyphleates are produced by a glycolipid biosynthetic pathway conserved across phylogenetically distant mycobacteria.	mycobacteria synthesize a variety of structurally related glycolipids with major biological functions. common themes have emerged for the biosynthesis of these glycolipids, including several families of proteins. genes encoding these proteins are usually clustered on bacterial chromosomal islets dedicated to the synthesis of one glycolipid family. here, we investigated the function of a cluster of five genes widely distributed across non-tuberculous mycobacteria. using defined mutant analysis an ...	2016	27028886
erratum for brown-elliott et al., utility of sequencing the erm(41) gene in isolates of mycobacterium abscessus subsp. abscessus with low and intermediate clarithromycin mics.		2016	27016591
persistent mycobacterium abscessus infection secondary to interferon-γ autoantibodies.		2016	27009440
prevalence and antimicrobial susceptibility of mycobacterium abscessus in a general hospital, china.	to gain greater insight into the prevalence drug resistant profiles of m. abscessus from a general hospital in beijing, china.	2016	27003165
epidemiology of rapidly growing mycobacteria bloodstream infections.	rapidly growing mycobacteria (rgm) bloodstream infections (bsi) are an emerging problem often associated with therapeutic challenges. we review the epidemiology, treatment and outcomes over a 5-year period of a heterogeneous group presenting to our institution with rgm bsi.	2016	26992253
update on pulmonary disease due to non-tuberculous mycobacteria.	non-tuberculous mycobacteria (ntm) are emerging worldwide as significant causes of chronic pulmonary infection, posing a number of challenges for both clinicians and researchers. while a number of studies worldwide have described an increasing prevalence of ntm pulmonary disease over time, population-based data are relatively sparse and subject to ascertainment bias. furthermore, the disease is geographically heterogeneous. while some species are commonly implicated worldwide (mycobacterium aviu ...	2016	26976549
analysis of pulmonary non-tuberculous mycobacterial infections after lung transplantation.	non-tuberculous mycobacteria (ntm) are important pathogens in lung transplant recipients. this study describes the spectrum of ntm respiratory tract infections and examines the association of ntm infections with lung transplant complications.	2016	27368989
medical management for the treatment of nontuberculous mycobacteria infection of the parotid gland: avoiding surgery may be possible.	infection with nontuberculous mycobacteria (ntm) is uncommon in the head and neck; therefore there is no clear consensus on treating these infections. our objective was to report our experience with a unique case of ntm infection of the parotid in an immunocompetent patient, in order to determine appropriate management through our experience with this pathology. a 57-year-old man, known for numerous comorbid diseases, presented to our institution complaining of right parotid swelling and pain. a ...	2016	27340407
identification of lysine acetylation in mycobacterium abscessus using lc-ms/ms after immunoprecipitation.	mycobacterium abscessus (mab), which manifests in the pulmonary system, is one of the neglected causes of nontuberculous mycobacteria (ntm) infection. treatment against mab is difficult, characterized by its intrinsic antibiotic drug resistance. lysine acetylation can alter the physiochemical property of proteins in living organisms. this study aimed to determine if this protein post-translational modification (ptm) exists in a clinical isolate m. abscessus gz002. we used the antiacetyl-lysine i ...	2016	27323652
oral macrolide therapy following short-term combination antibiotic treatment of mycobacterium massiliense lung disease.	although mycobacterium massiliense lung disease is increasing in patients with cystic fibrosis and non-cystic fibrosis bronchiectasis, optimal treatment regimens remain largely unknown. this study aimed to evaluate the efficacy of oral macrolide therapy after an initial 2-week course of combination antibiotics for the treatment of m massiliense lung disease.	2016	27167209
standardized interpretation of antibiotic susceptibility testing and resistance genotyping for mycobacterium abscessus with regard to subspecies and erm41 sequevar.	the objective of this study was to provide standardized antibiotic susceptibility testing (ast) for mycobacterium abscessus with regard to subspecies.	2016	27147307
in vitro comparison of ertapenem, meropenem, and imipenem against isolates of rapidly growing mycobacteria and nocardia by use of broth microdilution and etest.	we compared the activities of the carbapenems ertapenem, meropenem, and imipenem against 180 isolates of rapidly growing mycobacteria (rgm) and 170 isolates of nocardia using the clinical and laboratory standards institute (clsi) guidelines. a subset of isolates was tested using the etest. the rate of susceptibility to ertapenem and meropenem was limited and less than that to imipenem for the rgm. analysis of major and minor discrepancies revealed that >90% of the isolates of nocardia had higher ...	2016	27053677
a new piperidinol derivative targeting mycolic acid transport in mycobacterium abscessus.	the natural resistance of mycobacterium abscessus to most commonly available antibiotics seriously limits chemotherapeutic treatment options, which is particularly challenging for cystic fibrosis patients infected with this rapid-growing mycobacterium. new drugs with novel molecular targets are urgently needed against this emerging pathogen. however, the discovery of such new chemotypes has not been appropriately performed. here, we demonstrate the utility of a phenotypic screen for bactericidal ...	2016	27121350
whole-genome sequencing and comparative analysis of mycobacterium brisbanense reveals a possible soil origin and capability in fertiliser synthesis.	mycobacterium brisbanense is a member of mycobacterium fortuitum third biovariant complex, which includes rapidly growing mycobacterium spp. that normally inhabit soil, dust and water, and can sometimes cause respiratory tract infections in humans. we present the first whole-genome analysis of m. brisbanense um_wwy which was isolated from a 70-year-old malaysian patient. molecular phylogenetic analyses confirmed the identification of this strain as m. brisbanense and showed that it has an unusua ...	2016	27031249
genotype ntm-dr for identifying mycobacterium abscessus subspecies and determining molecular resistance.	we studied the performance of a new line probe assay for identifying the subspecies and determining the macrolide and aminoglycoside resistance levels of 50 mycobacterium abscessus isolates. agreement of genotype ntm-dr results with sequencing and phenotypic resistance results was 92% for subspecies identification and 98% for determining molecular and phenotypic resistance.	2016	27030487
antibiotic treatment for nontuberculous mycobacterial lung disease.	pulmonary infections are the most frequent diseases caused by nontuberculous mycobacteria (ntm). common causative organisms of pulmonary infection are slowly growing mycobacteria including mycobacterium avium complex and mycobacterium kansasii, and rapidly growing mycobacteria including mycobacterium abscessus complex. clinical concern has been raised over the increasing incidence of ntm lung disease combined with the poor treatment outcomes of these chronic infectious diseases. since treatment ...	2016	26967761
[rapid identification of mycobacterium abscessus in cystic fibrosis patients].		2016	26946526
a novel therapeutic approach using mesenchymal stem cells to protect against mycobacterium abscessus.	recent studies have demonstrated the therapeutic potential of mesenchymal stem cells (mscs) for the treatment of acute inflammatory injury and bacterial pneumonia, but their therapeutic applications in mycobacterial infections have not been investigated. in this study, we demonstrated the use of mscs as a novel therapeutic strategy against mycobacterium abscessus (m. abscessus), which is the most drug-resistant and difficult-to-treat mycobacterial pathogen. the systemic intravenous injection of ...	2016	26946350
antimycobacterial activity of a new peptide polydim-i isolated from neotropical social wasp polybia dimorpha.	mycobacterium abscessus subsp. massiliense, a rapidly growing mycobacteria (rgm) that is becoming increasingly important among human infectious diseases, is virulent and pathogenic and presents intrinsic resistance to several antimicrobial drugs that might hamper their elimination. therefore, the identification of new drugs to improve the current treatment or lower the risk of inducing resistance is urgently needed. wasp venom primarily comprises peptides that are responsible for most of the bio ...	2016	26930596
[a case of pulmonary mycobacterium abscessus infection that developed during immunosuppressive therapy for myasthenia gravis with recurrent thymoma].	a 58-year-old man developed cough, sputum, and low-grade fever during immunosuppressive treatment with corticosteroids and cyclosporine for myasthenia gravis with recurrent thymoma. since chest ct revealed diffuse nodular opacities in both lung fields, he was referred to our department. mycobacterium abscessus was repeatedly cultured from his sputum, and he was diagnosed with pulmonary m. abscessus infection. although both chest radiological findings and clinical symptoms were mild, he required ...	2016	27263226
rapid-growing mycobacteria infections in medical tourists: our experience and literature review.	"medical tourism" has gained popularity over the past few decades. this is particularly common with patients seeking elective cosmetic surgery in the developing world. however, the risk of severe and unusual infectious complications appears to be higher than for patients undergoing similar procedures in the united states.	2016	27095310
pulmonary mycobacterium abscessus infection in a patient with triple a syndrome.	gastroesophageal disorders such as achalasia can be associated with pulmonary disorders because of non-tuberculous mycobacteria, frequently masquerading as aspiration pneumonia. the optimal therapeutic regimen and duration of treatment for non-tuberculous mycobacteria lung disease is not well established. here, we present an 11 year old male patient with mycobacterium abscessus pulmonary disease and underlying triple a syndrome, who was successfully treated with 2 months of imipenem, amikacin, c ...	2016	27080471
nontuberculous mycobacteria in cystic fibrosis patients on the island of gran canaria. a population study.	to determine the prevalence of nontuberculous mycobacteria (ntm) colonization and disease in cystic fibrosis (cf) patients.	2016	27262751
isolation of rapidly growing nontuberculous mycobacteria in wounds following combat-related injury.	rapidly growing nontuberculous mycobacteria (rgntm) have yet to be described in combat-related injuries. this study investigates the epidemiology, clinical findings, treatment, and outcomes of rgntm infections among combat casualties wounded in afghanistan from 2010 to 2012.	2016	27244062
mycobacterium abscessus as a cause of chronic meningitis: a rare clinical entity.		2016	27079354
moxifloxacin's limited efficacy in the hollow-fiber model of mycobacterium abscessus disease.	current regimens used to treat pulmonary mycobacterium abscessus disease have limited efficacy. there is an urgent need for new drugs and optimized combinations and doses. we performed hollow-fiber-system studies in which m. abscessus was exposed to moxifloxacin lung concentration-time profiles similar to human doses of between 0 and 800 mg/day. the minimum bactericidal concentration and mic were 8 and 2 mg/liter, respectively, in our m. abscessus strain, suggesting bactericidal activity. measur ...	2016	27067317
acquisition of mycobacterium abscessus among ventilator-dependent patients in taiwan chronic respiratory care facilities.	to investigate the acquisition of mycobacterium abscessus among ventilator-dependent patients.	2016	27064349
notes from the field: mycobacterium abscessus infections among patients of a pediatric dentistry practice--georgia, 2015.	on september 13, 2015, the georgia department of public health (dph) was notified by hospital a of a cluster of pediatric mycobacterium abscessus odontogenic infections. hospital a had provided care for nine children who developed presumptive or confirmed m. abscessus infection after having a pulpotomy at pediatric dentistry practice a (dates of onset: july 23, 2014-september 4, 2015). during a pulpotomy procedure, decay and the diseased pulp are removed to preserve a deciduous tooth. dph initia ...	2016	27054966
investigation of mycobacterium abscessus outbreak among cystic fibrosis patients, hawaii 2012.		2016	27238609
gastric lap-band infection due to mycobacterium abscessus presenting as new-onset ascites in a cirrhotic patient.	nontuberculous mycobacteria are ubiquitous environmental organisms that are infrequently implicated as pathogens. peritoneal infection with nontuberculous mycobacteria is rare and published reports are most commonly associated with peritoneal dialysis. this study describes a case of a 41-year-old woman with cirrhosis who had mycobacterium abscessus peritonitis and an abdominal abscess resulting from infection of a remotely placed gastric band (lap-band; apollo endosurgery, inc).	2016	27222118
fluorescent reporter ds6a mycobacteriophages reveal unique variations in infectibility and phage production in mycobacteria.	mycobacteriophage ds6a is unique among the more than 8,000 isolated mycobacteriophages due to its ability to form plaques exclusively on mycobacteria belonging to the mycobacterium tuberculosis complex (mtbc). speculation surrounding this specificity has led to unsupported assertions in published studies and patents that nontuberculous mycobacteria (ntm) are wholly resistant to ds6a infection. in this study, we identified two independent nonessential regions in the ds6a genome and replaced them ...	2016	27672191
evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of mycobacterium abscessus subspecies according to whole-genome sequencing.	this study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry with the vitek ms plus system for identifying mycobacterium abscessus subspecies in order to facilitate more rapid and appropriate therapy. a total of 175 clinical m. abscessus strains were identified by whole-genome sequencing analysis: 139 mycobacterium abscessus subsp. abscessus and 36 mycobacterium abscessus subsp. massiliense the research-use-only (ruo) saramis k ...	2016	27682129
the distinct fate of smooth and rough mycobacterium abscessus variants inside macrophages.	mycobacterium abscessus is a pathogenic, rapidly growing mycobacterium responsible for pulmonary and cutaneous infections in immunocompetent patients and in patients with mendelian disorders, such as cystic fibrosis (cf). mycobacterium abscessus is known to transition from a smooth (s) morphotype with cell surface-associated glycopeptidolipids (gpl) to a rough (r) morphotype lacking gpl. herein, we show that m. abscessus s and r variants are able to grow inside macrophages and are present in mor ...	2016	27906132
eradication of mycobacterium abscessus pulmonary infection in a child with idiopathic bronchiectasis.		2016	27896296
use of an experimental model to evaluate infection resistance of meshes in abdominal wall surgery.	staphylococcal species are the most common organisms causing prosthetic mesh infections, however, infections due to rapidly growing mycobacteria are increasing. this study evaluates the resistance of biomaterial for abdominal wall prostheses against the development of postoperative infection in a rat model.	2016	27884340
prevalence and species spectrum of both pulmonary and extrapulmonary nontuberculous mycobacteria isolates at a tertiary care center.	nontuberculous mycobacteria (ntm) infection associated with pulmonary and extrapulmonary disease has been increasing globally. despite an increase in incidence rate of ntm infection, its prevalence, species diversity, and circulation pattern in india is largely unknown. this study sought to investigate the overall burden and diversity of ntm among both pulmonary and extrapulmonary clinical isolates from a northern indian population.	2016	27847012
emergence and spread of a human-transmissible multidrug-resistant nontuberculous mycobacterium.	lung infections with mycobacterium abscessus, a species of multidrug-resistant nontuberculous mycobacteria, are emerging as an important global threat to individuals with cystic fibrosis (cf), in whom m. abscessus accelerates inflammatory lung damage, leading to increased morbidity and mortality. previously, m. abscessus was thought to be independently acquired by susceptible individuals from the environment. however, using whole-genome analysis of a global collection of clinical isolates, we sh ...	2016	27846606
structure-guided, target-based drug discovery - exploiting genome information from hiv to mycobacterial infections.	the use of protein crystallography in structure-guided drug discovery allows identification of potential inhibitor-binding sites and optimisation of interactions of hits and lead compounds with a target protein. an early example of this approach was the use of the structure of hiv protease in designing aids antivirals. more recently, use of structure-guided design with fragment-based drug discovery, which reduces the size of screening libraries by decreasing complexity, has improved ligand effic ...	2016	28132480
mycobacterium abscessus complex bacteremia due to prostatitis after prostate biopsy.	we present the case of a 49-year-old man, who developed mycobacterium abscessus complex (m. abscessus complex) bacteremia and prostatitis after prostate biopsy. the patient was successfully treated with amikacin with imipenem-cilastatin with clarithromycin. infections caused by m. abscessus complex have been increasingly described as a complication associated with many invasive procedures. invasive procedures might have contributed to the occurrence of the m. abscessus complex. although m. absce ...	2016	27998503
trfa1 gene in clinical isolates of mycobacterium tuberculosis.	the incidence of resistant strains of mycobacterium tuberculosis (mtb), including multi-drug resistant, extensively drug resistant, or totally drug resistant, is one of the major problems of health policies worldwide. the accumulation of mutations causes multi-drug resistant strains. mycobacterium abscessus has a plasmid called pmab2401 containing the trfa1 gene in its integron part. the aim of the present study was to investigate the possible existence of the trfa1 gene in clinical strains of m ...	2016	28043564
identification of nontuberculous mycobacteria by high-performance liquid chromatography from patients in tehran.	the genus mycobacterium contains over 140 species comprises pathogenic and nonpathogenic strains. nontuberculous mycobacteria (ntm) causing clinical disease have become increasingly common and more diverse. widespread features of ntm infection can make the diagnosis difficult. precise species-level detection can aid in distinguishing environmental contamination from actual infection and, furthermore, can assist in making a choice of antimicrobial therapy.	2016	28043562
antibiotic treatment for nontuberculous mycobacteria lung infection in people with cystic fibrosis.	nontuberculous mycobacteria are mycobacteria, other than those in the mycobacterium tuberculosis complex, and are commonly found in the environment. nontuberculous mycobacteria species (most commonly mycobacterium avium complex and mycobacterium abscessus) are isolated from the respiratory tract of approximately 5% to 40% of individuals with cystic fibrosis; they can cause lung disease in people with cystic fibrosis leading to more a rapid decline in lung function and even death in certain circu ...	2016	28000919
complete genome sequencing and comparative analyses of broad-spectrum antimicrobial-producing micromonospora sp. hk10.	micromonospora genus produces >700 bioactive compounds of medical relevance. in spite of its ability to produce high number of bioactive compounds, no genome sequence is available with comprehensive secondary metabolite gene clusters analysis for anti-microbial producing micromonospora strains. thus, here we contribute the full genome sequence of micromonospora sp. hk10 strain, which has high antibacterial activity against several important human pathogens like, mycobacterium abscessus, mycobact ...	2016	27609432
a novel culture medium for isolation of rapidly-growing mycobacteria from the sputum of patients with cystic fibrosis.	isolation of mycobacteria from the sputum of patients with cystic fibrosis (cf) is challenging due to the overgrowth of cultures by other bacteria and fungi. in this setting, burkholderia cepacia selective agar (bcsa) has been recommended as a convenient and effective culture medium for the isolation of rapidly-growing, non-tuberculous mycobacteria (ntm). a novel selective culture medium (rgm medium) was evaluated for the isolation of rapidly-growing ntm from the sputum of children and adults wi ...	2016	26002312
functional characterization of the mycobacterium abscessus genome coupled with condition specific transcriptomics reveals conserved molecular strategies for host adaptation and persistence.	mycobacterium abscessus subsp. abscessus (mab) is a highly drug resistant mycobacterium and the most common respiratory pathogen among the rapidly growing non-tuberculous mycobacteria. mab is also one of the most deadly of the emerging cystic fibrosis (cf) pathogens requiring prolonged treatment with multiple antibiotics. in addition to its "mycobacterial" virulence genes, the genome of mab harbours a large accessory genome, presumably acquired via lateral gene transfer including homologs shared ...	2016	27495169
structure elucidation and biological activity of antibacterial compound from micromonospora auratinigra, a soil actinomycetes.	the aim of this study was to isolate and characterize the bioactive compound of micromonospora auratinigra, hk-10 and its antibacterial inhibitory mechanism.	2016	27406903
nontuberculous mycobacteria in cystic fibrosis.	nontuberculous mycobacteria (ntm) are found in approximately 10 % of cystic fibrosis (cf) patients, but only a portion will develop ntm disease. the management of cf lung disease should be optimized, including antibiotic therapy targeted to the individual's usual airway bacteria, prior to considering treatment for ntm lung disease. those who meet criteria for ntm lung disease may not necessarily require treatment and could be monitored expectantly if symptoms and radiographic findings are minima ...	2016	28035194
comparing the harmful effects of nontuberculous mycobacteria and gram negative bacteria on lung function in patients with cystic fibrosis.	to better understand the relative effects of infection with nontuberculous mycobacteria and gram negative bacteria on lung function decline in cystic fibrosis, we assessed the impact of each infection in a danish setting.	2016	26482717
assessment of the microbial constituents of the home environment of individuals with cystic fibrosis (cf) and their association with lower airways infections.	cystic fibrosis (cf) airways are colonized by a polymicrobial community of organisms, termed the cf microbiota. we sought to define the microbial constituents of the home environment of individuals with cf and determine if it may serve as a latent reservoir for infection.	2016	26859493
vaccine strategies against bacterial pathogens in cystic fibrosis patients.	a large number of cystic fibrosis pathogens such as bacteria of the burkholderia cepacia complex, pseudomonas aeruginosa, or mycobacterium abscessus are associated with complex therapeutic problems due to their inherent resistance to antibiotics. no vaccine is currently available against those pathogens. vaccines are therefore crucial to combat these multidrug-resistant bacteria in specific clinical situations including cystic fibrosis. various strategies may be considered to develop these vacci ...	2016	26746326
emended description of mycobacterium abscessus, mycobacterium abscessus subsp. abscessus and mycobacteriumabscessus subsp. bolletii and designation of mycobacteriumabscessus subsp. massiliense comb. nov.	the taxonomic position of members of the mycobacterium abscessus complex has been the subject of intensive investigation and, in some aspects confusion, in recent years as a result of varying approaches to genetic data interpretation. currently, the former species mycobacterium massiliense and mycobacterium bolletii are grouped together as mycobacterium abscessus subsp. bolletii. they differ greatly, however, as the former m. bolletii has a functional erm(41) gene that confers inducible resistan ...	2016	27499141
complete genome sequence of mycobacterium abscessus subsp. bolletii.	we report the complete genome sequence of a mycobacterium abscessus subsp. bolletii isolate recovered from a sputum culture from an individual with cystic fibrosis. this sequence is the first completed whole-genome sequence of m. abscessus subsp. bolletii and adds value to studies of m. abscessus complex genomics.	2016	27284156
insights into the smooth-to-rough transitioning in mycobacterium bolletii unravels a functional tyr residue conserved in all mycobacterial mmpl family members.	in mycobacteria, mmpl proteins represent key components that participate in the biosynthesis of the complex cell envelope. whole genome analysis of a spontaneous rough morphotype variant of mycobacterium abscessus subsp. bolletii identified a conserved tyrosine that is crucial for the function of mmpl family proteins. isogenic smooth (s) and rough (r) variants differed by a single mutation linked to a y842h substitution in mmpl4a. this mutation caused a deficiency in glycopeptidolipid production ...	2016	26585558
pseudooutbreak of rapidly growing mycobacteria due to mycobacterium abscessus subsp bolletii in a digestive and respiratory endoscopy unit caused by the same clone as that of a countrywide outbreak.	the nontuberculous mycobacteria (ntm) are widely spread. in brazil, 2,520 cases of rapidly growing mycobacteria (rgm) infections after medical procedures were reported, with 5.4% of cases related to nonsurgical invasive procedures and with an occurrence of 1 clone (bra100) of mycobacterium abscessus subsp bolletii.	2016	27524260
a cluster of endophthalmitis caused by mycobacterium abscessus after cataract surgery.	we report two cases of postoperative endophthalmitis after cataract surgery caused by the same strain of mycobacterium abscessus confirmed by arbitrarily primed polymerase chain reaction, sequencing of the erythromycin ribosome methyltransferase gene and pulsed-field gel electrophoresis. the outcomes were poor despite aggressive treatments. this is the first report of nontuberculous mycobacteria as a causative pathogen for a cluster of endophthalmitis.	2016	24657068
postcesarean section wound infection caused by mycobacterium massiliense.	mycobacterium abscessus subsp. massiliense (a subspecies of the m. abscessus complex) is a rare causative agent of surgical site infection after cesarean section (c section). we tried to seek the common source of infection and unravel the optimal treatment modalities.	2016	26350122
synthesis and biological activity of alkynoic acids derivatives against mycobacteria.	2-alkynoic acids have bactericidal activity against mycobacterium smegmatis but their activity fall sharply as the length of the carbon chain increased. in this study, derivatives of 2-alkynoic acids were synthesized and tested against fast- and slow-growing mycobacteria. their activity was first evaluated in m. smegmatis against their parental 2-alkynoic acids, as well as isoniazid, a first-line antituberculosis drug. the introduction of additional unsaturation or heteroatoms into the carbon ch ...	2016	26256431
[pulmonary mycobacterium abscessus sensu lato infection in patients without cystic fibrosis].	infection with mycobacterium abscessus sensu lato is uncommon in patients without cystic fibrosis. we are interested in these patients and have collected cases in finistère between 2007 and 2011.	2016	26238926
molecular epidemiology of mycobacterium abscessus complex isolates in ireland.	the mycobacterium abscessus complex are the rapidly growing mycobacteria (rgm) most commonly causing lung disease, especially in cystic fibrosis (cf) patients. ireland has the world's highest cf incidence. the molecular epidemiology of m. abscessus complex in ireland is unreported.	2016	26072272
mycobacterium abscessus ventriculoperitoneal shunt infection and review of the literature.	a 30-year-old man with history of neonatal hydrocephalus requiring ventriculoperitoneal shunt placement presented with mycobacterium abscessus shunt infection despite no shunt manipulation over 10 years prior to presentation. cure was not achieved until complete removal of all cns shunt foreign body was performed despite initial adequate antimicrobial therapy.	2016	26148928
prolonged postprocedural outbreak of mycobacterium massiliense infections associated with ultrasound transmission gel.	postprocedural infections by mycobacterium abscessus complex are increasing worldwide, and the source and route of transmission are infrequently identified. here the extension of a previous clustering of paediatric patients with surgical site infections due to a single strain of the subspecies m. massiliense is reported. the investigation was conducted at a 2200-bed teaching hospital in taiwan and included microbial surveillance of the environment (water, air, equipment and supplies) and a case- ...	2016	26794030
an evaluation of different steam disinfection protocols for cystic fibrosis nebulizers.	contamination is a key element in cystic fibrosis. for this reason, nebulizer hygiene is an important, but complex and time-consuming task for cystic fibrosis patients. the aim of this study was to compare different steam disinfection and drying protocols.	2016	26233897
mycobacterium abscessus pulmonary infection during hepatitis c treatment with telaprevir, peginterferon and ribavirin.	the first generation protease inhibitors has been the mainstay of hepatitis c treatment for the last couple of years, showing marked improvement in sustained virological response, but also increased side effects. infection has emerged as a common complication of telaprevir and boceprevir in combination with peginterferon and ribavirin, usually caused by common pathogens. we present the case of a 65 years old man who developed a mycobacterium abscessus pulmonary infection during treatment with te ...	2016	25536652
frequency and clinical significance of respiratory isolates of non-tuberculous mycobacteria in riyadh, saudi arabia.	the prevalence of pulmonary non-tuberculous mycobacteria (ntm) disease is increasing worldwide. the aim of this study was to assess the frequency and clinical significance of ntm isolated from respiratory specimens at king abdul-aziz medical city-riyadh, saudi arabia.	2016	25130951
treatment of non-tuberculous mycobacterial lung disease.	treatment of non-tuberculous mycobacterial lung disease (ntm-ld) is challenging for several reasons including the relative resistance of ntm to currently available drugs and the difficulty in tolerating prolonged treatment with multiple drugs. yet-to-be-done, large, multicenter, prospective randomized studies to establish the best regimens will also be arduous because multiple ntm species are known to cause human lung disease, differences in virulence and response to treatment between different ...	2016	28529461
effectiveness of current disinfection procedures against biofilm on contaminated gi endoscopes.	attention to patient safety has increased recently due to outbreaks of nosocomial infections associated with gi endoscopy. the aim of this study was to evaluate current cleaning and disinfection procedures of endoscope channels with high bioburden and biofilm analysis, including the use of resistant mycobacteria associated with postsurgical infections in brazil.	2016	26408426
comparing mycobacterium massiliense and mycobacterium abscessus lung infections in cystic fibrosis patients.	mycobacterium massiliense is closely related to mycobacterium abscessus and is also a frequent cause of mycobacterial lung disease in patients with cystic fibrosis (cf). there has been no previous investigation of possible differences between m. massiliense and m. abscessus infections in the setting of cf.	2015	25085077
increasing nontuberculous mycobacteria infection in cystic fibrosis.	nontuberculous mycobacteria (ntm) are emerging infections in the cf population.	2015	24917112
a novel inhibitor of gyrase b is a potent drug candidate for treatment of tuberculosis and nontuberculosis mycobacterial infections.	new drugs to treat drug-resistant tuberculosis are urgently needed. extensively drug-resistant and probably the totally drug-resistant tuberculosis strains are resistant to fluoroquinolones like moxifloxacin, which target gyrase a, and most people infected with these strains die within a year. in this study, we found that a novel aminobenzimidazole, vxc-486, which targets gyrase b, potently inhibits multiple drug-sensitive isolates and drug-resistant isolates of mycobacterium tuberculosis in vit ...	2015	25534737
several siblings with cystic fibrosis as a risk factor for poor outcome.	occurrence of cystic fibrosis (cf) in more than one member in a family is not uncommon. the aim of our study was to assess the influence of multiple siblings with cf on disease expression and outcome.	2015	25528949
β-lactamase inhibition by avibactam in mycobacterium abscessus.	two β-lactams, cefoxitin and imipenem, are part of the reference treatment for pulmonary infections with mycobacterium abscessus. m. abscessus has recently been shown to produce a broad-spectrum β-lactamase, blamab, indicating that the combination of β-lactams with a blamab inhibitor may improve treatment efficacy. the objectives of this study were to evaluate the impact of blamab production on the efficacy of β-lactams in vitro and to assess the benefit of blamab inhibition on the activity of β ...	2015	25525201
the drug susceptibility profile and inducible resistance to macrolides of mycobacterium abscessus and mycobacterium massiliense in korea.	we conducted drug susceptibility testing (dst) against various antimicrobial agents, including new candidate drugs, and investigated the relationship between inducible resistance (ir) to macrolides and erm(41) gene in mycobacterium abscessus complex. sixty-two isolates of m. abscessus complex from 2 tertiary care hospitals in south korea were tested against 10 antimicrobial agents. thirty-five isolates were m. abscessus, and 27 were mycobacterium massiliense. amikacin, moxifloxacin, linezolid, c ...	2015	25467784
whole-genome sequencing and epidemiological analysis do not provide evidence for cross-transmission of mycobacterium abscessus in a cohort of pediatric cystic fibrosis patients.	mycobacterium abscessus has emerged as a major pathogen in cystic fibrosis (cf) patients and has been associated with poor clinical outcomes, particularly following lung transplant. we investigated the acquisition of this bacterium in a cohort of pediatric cf patients.	2015	25452595
risk factors for microbiologic failure among taiwanese adults with mycobacterium abscessus complex pulmonary disease.	the mycobacterium abscessus complex is a common cause of pulmonary nontuberculous mycobacteria infections in taiwan. we examined the risk factors associated with treatment outcome in taiwanese adults with pulmonary disease caused by the m. abscessus complex.	2015	25440976
evaluation of pcr-reverse blot hybridization assay for the differentiation and identification of mycobacterium species in liquid cultures.	the purpose of this study was to evaluate the usefulness of reba myco-id(®) assay based on the principle of reverse blot hybridization assay (reba) for the rapid detection of mycobacterium tuberculosis (mtb), the differentiation of mtb from nontuberculous mycobacteria (ntm) and the identification of ntm species in liquid cultures.	2015	25346202
intracranial mycobacterium abscessus infection in a healthy toddler.	we present the first case of pediatric intracranial mycobacterium abscessus infection in a 16-month-old female with neurofibromatosis type 1. we describe a successful treatment regimen including excisional biopsy combined with high-dose steroids and 16 weeks of triple antimicrobial therapy that resulted in clinical cure and an excellent neurologic outcome.	2015	25144796
post liposuction mycobacterium abscessus surgical site infection in a returned medical tourist complicated by a paradoxical reaction during treatment.	rapidly growing mycobacterial skin and soft tissue infections are known to complicate cosmetic surgical procedures. treatment consists of more surgery and prolonged antibiotic therapy guided by drug susceptibility testing. paradoxical reactions occurring during antibiotic therapy can further complicate treatment of non-tuberculous mycobacterial infections. we report a case of post liposuction mycobacterium abscessus surgical site infection in a returned medical tourist and occurrence of paradox ...	2015	26753088
time-kill kinetics of antibiotics active against rapidly growing mycobacteria.	this study was conducted to generate basic pharmacodynamic information on the relationship between antibiotic concentrations and the growth of rapidly growing mycobacteria (rgm), and thereby contribute to a better understanding of current and future drug regimens for diseases caused by rgm.	2015	25344808
evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria.	nontuberculous mycobacteria (ntm) causing human infectious disease have become increasingly common. rapid and accurate identification to the species level is, therefore, critical. the speed-oligo mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. the present study aimed to evaluate the performance of the speed-oligo mycobacteria assay for the identification of ntm. we examined a total of 62 strains (9 ...	2015	25596120