Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| comparison of three real-time pcr methods with blood smears and rapid diagnostic test in plasmodium sp. infection. | in cases of malaria, rapid and accurate diagnosis of plasmodium sp. is essential. in this study three different quantitative, real-time pcr methods were compared with routine methods used for malaria diagnosis. a comparative study was conducted prospectively in the laboratories of montpellier and nîmes university hospitals. the methods used for routine diagnostic malaria testing consisted of microscopic examination of giemsa-stained blood smears and rapid diagnostic tests. three quantitative rea ... | 2010 | 19840032 |
| evaluation of rapid diagnostic tests for malaria case management in gabon. | a laboratory-confirmed diagnosis is the basis of malaria case management. rapid diagnostic tests (rdts) create new opportunities for improved care in endemic areas. diagnostic performance of optimal-it and acon was assessed in comparison with microscopy at 2 sites in gabon. between february 2008 and january 2009, 2125 febrile children under 11 years old were diagnosed using microscopy and rdts. plasmodial infection was detected more frequently using acon (27%) and optimal-it (27%) compared to mi ... | 2010 | 19846265 |
| kir3dl1/s1 genotypes and kir2ds4 allelic variants in the ab kir genotypes are associated with plasmodium-positive individuals in malaria infection. | the importance of innate immunity in malaria has been suggested for early protection from maturation and multiplication of plasmodium parasites injected via infected mosquitoes. in this study, the killer cell immunoglobulin-like receptor (kir) genes in innate immunity were investigated for an association with malaria in the comparison between plasmodium-positive and plasmodium-negative melanesian individuals in the solomon islands, one of the most hyperendemic malaria regions in the world. the h ... | 2010 | 19859704 |
| [epidemiological baseline situation before the construction of a small dam in five villages of bouaké, central côte-d'ivoire]. | abstract from june 2007 to june 2008, entomological, malacological and parasitological investigations were carried out in five villages in close proximity to a small dam of raf-fierkro in bouaké, central côte-d'ivoire. the objective of the study was to identify vectors and intermediate host snails of parasitic diseases related to water, and to assess the prevalence of malaria, schistosomiasis and soil-transmitted helminthiasis before dam construction. mosquitoes were caught by human landing catc ... | 2010 | 20084484 |
| computational analysis of binding between malarial dihydrofolate reductases and anti-folates. | plasmodium falciparum readily develops resistance to the anti-folates pyrimethamine and proguanil via a characteristic set of mutations in the dihydrofolate reductase (pfdhfr) gene that leads to reduced competitive drug binding at the enzyme's active site. analogous mutations can be found in the dhfr gene in isolates of plasmodium vivax (pvdhfr) although anti-folates have not been widely used for the treatment of this infection. here the interactions between dhfr inhibitors and modelled structur ... | 2010 | 20193090 |
| what is the evidence for the existence of plasmodium ovale hypnozoites? | relapsing human malaria is regarded to be caused by plasmodium vivax and plasmodium ovale. p. vivax relapses originate from dormant liver stages: "hypnozoites". also, p. ovale, a species considered as closely related to p. vivax, is in analogy assumed to display hypnozoites. a close biologic relationship is, however, not supported by molecular genetic studies. therefore, original literature published since the description of p. ovale in 1922 was systematically screened for the demonstration of h ... | 2010 | 20922429 |
| plasmodium ovale infection in malaysia: first imported case. | plasmodium ovale infection is rarely reported in malaysia. this is the first imported case of p. ovale infection in malaysia which was initially misdiagnosed as plasmodium vivax. | 2010 | 20929588 |
| in vivo and in vitro efficacy of chloroquine against plasmodium malariae and p. ovale in papua, indonesia. | reports of potential drug-resistant strains of plasmodium malariae in western indonesia raise concerns that chloroquine resistance may be emerging in p. malariae and p. ovale. in order to assess this, in vivo and in vitro efficacy studies were conducted in patients with monoinfection in papua, indonesia. consecutive patients with uncomplicated malaria due to p. ovale or p. malariae were enrolled in a prospective clinical trial, provided with supervised chloroquine treatment, and followed for 28 ... | 2010 | 20937779 |
| splenic infarction during plasmodium ovale acute malaria: first case reported. | the splenic complications of acute malaria include two different prognostic and treatment entities: splenic infarction and splenic rupture. this is the first case of splenic infarction during an acute malaria due to plasmodium ovale in a 34-year-old man. as in the majority other described cases of splenic infarction, the course was spontaneously favourable, suggesting that this complication was relatively benign compared to splenic rupture, which is life-threatening and usually necessitating sur ... | 2010 | 20955610 |
| transfusion-related plasmodium ovale malaria complicated by acute respiratory distress syndrome (ards) in a non-endemic country. | 46year old female presented with a one week history of high grade fever, chills, cough, and severe nausea. the patient had been admitted a month earlier with severe lower gastrointestinal bleeding from hemorrhoids necessitating transfusion of 7 units of packed red blood cells. initial work-up was unremarkable. because of persistent symptoms, the patient was admitted 2 days later. malaria smear was positive. due to the severity of her symptoms, she was managed as falciparum malaria and was starte ... | 2010 | 20971212 |
| five-minute giemsa stain for rapid detection of malaria parasites in blood smears. | the giemsa stain is used as the gold standard for the diagnosis of malaria on blood smears. the classical staining procedure requires between 30 and 45 min. we modified the giemsa stain and reduced the staining time to 5 min without any loss of quality. | 2010 | 21088023 |
| a taqman real-time pcr assay for the detection and quantitation of plasmodium knowlesi. | the misdiagnosis of plasmodium knowlesi by microscopy has prompted a re-evaluation of the geographic distribution, prevalence and pathogenesis of this species using molecular diagnostic tools. in this report, a specific probe for p. knowlesi, that can be used in a previously described taqman real-time pcr assay for detection of plasmodium spp., and plasmodium falciparum, plasmodium vivax, plasmodium malariae and plasmodium ovale, was designed and validated against clinical samples. | 2010 | 21114872 |
| the challenge of diagnosing plasmodium ovale malaria in travellers: report of six clustered cases in french soldiers returning from west africa. | plasmodium ovale is responsible for 5% of imported malaria in french travellers. the clinical and biological features of six clustered cases of p. ovale malaria in an army unit of 62 french soldiers returning from the ivory coast are reported. | 2010 | 21143962 |
| high-throughput molecular diagnosis of circumsporozoite variants vk210 and vk247 detects complex plasmodium vivax infections in malaria endemic populations in papua new guinea. | malaria is endemic in lowland and coastal regions of papua new guinea (png), and is caused by plasmodium falciparum, plasmodium vivax, plasmodium malariae and plasmodium ovale. infection by p. vivax is attributed to distinct strains, vk210 and vk247, which differ in the sequence of the circumsporozoite protein (pvcsp). here, based upon sequence polymorphisms in pvcsp, we developed a post-pcr ligation detection reaction-fluorescent microsphere assay (ldr-fma) to distinguish these p. vivax strains ... | 2010 | 21147267 |
| malaria transmission in two rural communities in the forest zone of ghana. | malaria transmission was assessed in two rural communities, kona and afamanaso in sekyere south district, ashanti region, in the forest zone of ghana to provide baseline data for ongoing clinical studies and the evaluation of the effect of interventions. altogether, 3,479 anopheles gambiae and 1,157 anopheles funestus were caught by human landing catches. sporozoite rates determined by either microscopy of salivary glands or enzyme-linked immunosorbent assay (elisa) for plasmodium falciparum in ... | 2010 | 21153839 |
| giemsa-stained thick blood films as a source of dna for plasmodium species-specific real-time pcr. | this study describes the use of thick blood films (tbf) as specimens for dna amplification with the plasmodium species-specific real-time pcr that was recently validated on whole blood samples. | 2010 | 21176207 |
| [plasmodium ovale malaria severity]. | 2010 | 21194860 | |
| the first case of plasmodium ovale malaria from bangladesh. | the authors report the first indigenous case of plasmodium ovale infection from bangladesh. the diagnosis was confirmed by pcr and sequence analysis. the patient had neither been outside of the country nor ever received blood transfusions. the authors concluded that there was evidence for a local transmission of p ovale malaria in bangladesh. p ovale malaria should therefore always be considered a potential differential diagnosis in the indigenous population as well as travellers and migrants re ... | 2010 | 22778371 |
| test characteristics of the sd fk80 plasmodium falciparum/plasmodium vivax malaria rapid diagnostic test in a non-endemic setting. | the sd fk80 p.f/p.v malaria antigen rapid test (standard diagnostics, korea) (fk80) is a three-band malaria rapid diagnostic test detecting plasmodium falciparum histidine-rich protein-2 (hrp-2) and plasmodium vivax-specific lactate dehydrogenase (pv-pldh). the present study assessed its performance in a non-endemic setting. | 2009 | 19930609 |
| evaluation of the palutop+4 malaria rapid diagnostic test in a non-endemic setting. | palutop+4 (all. diag, strasbourg, france), a four-band malaria rapid diagnostic test (malaria rdt) targeting the histidine-rich protein 2 (hrp-2), plasmodium vivax-specific parasite lactate dehydrogenase (pv-pldh) and pan plasmodium-specific pldh (pan-pldh) was evaluated in a non-endemic setting on stored whole blood samples from international travellers suspected of malaria. | 2009 | 20003378 |
| prevalence of plasmodium spp. in malaria asymptomatic african migrants assessed by nucleic acid sequence based amplification. | malaria is one of the most important infectious diseases in the world. although most cases are found distributed in the tropical regions of africa, asia, central and south americas, there is in europe a significant increase in the number of imported cases in non-endemic countries, in particular due to the higher mobility in today's society. | 2009 | 19138412 |
| high sensitivity detection of plasmodium species reveals positive correlations between infections of different species, shifts in age distribution and reduced local variation in papua new guinea. | when diagnosed by standard light microscopy (lm), malaria prevalence can vary significantly between sites, even at local scale, and mixed species infections are consistently less common than expect in areas co-endemic for plasmodium falciparum, plasmodium vivax and plasmodium malariae. the development of a high-throughput molecular species diagnostic assay now enables routine pcr-based surveillance of malaria infections in large field and intervention studies, and improves resolution of species ... | 2009 | 19284594 |
| [false-negative result with the rapid diagnostic test in plasmodium ovale infection]. | 2009 | 19386395 | |
| towards high-throughput molecular detection of plasmodium: new approaches and molecular markers. | several strategies are currently deployed in many countries in the tropics to strengthen malaria control toward malaria elimination. to measure the impact of any intervention, there is a need to detect malaria properly. mostly, decisions still rely on microscopy diagnosis. but sensitive diagnosis tools enabling to deal with a large number of samples are needed. the molecular detection approach offers a much higher sensitivity, and the flexibility to be automated and upgraded. | 2009 | 19402894 |
| pathological rupture of the spleen in malaria: analysis of 55 cases (1958-2008). | splenic rupture during acute malaria is rare but underreported. because splenic rupture occurs mostly in non-immune adults, ongoing malaria elimination efforts may paradoxically increase the proportion of plasmodium-infected patients suffering from this life-threatening complication. the pathogenesis and optimal patient management are still debated. | 2009 | 19411041 |
| test characteristics of two rapid antigen detection tests (sd fk50 and sd fk60) for the diagnosis of malaria in returned travellers. | two malaria rapid diagnostic tests were evaluated in a travel clinic setting: the sd fk50 malaria ag plasmodium falciparum test (a two-band test) and the sd fk60 malaria ag p. falciparum/pan test (a three-band test). | 2009 | 19416497 |
| preliminary study of malaria incidence in nouakchott, mauritania. | malaria is one of the main motives for outpatient consultation and hospitalization in mauritania. however, its incidence remains unclear because of diagnostic problems and insufficient epidemiological data. | 2009 | 19416545 |
| chimpanzee malaria parasites related to plasmodium ovale in africa. | since the 1970's, the diversity of plasmodium parasites in african great apes has been neglected. surprisingly, p. reichenowi, a chimpanzee parasite, is the only such parasite to have been molecularly characterized. this parasite is closely phylogenetically related to p. falciparum, the principal cause of the greatest malaria burden in humans. studies of malaria parasites from anthropoid primates may provide relevant phylogenetic information, improving our understanding of the origin and evoluti ... | 2009 | 19436742 |
| evaluation of the sd fk70 malaria ag plasmodium vivax rapid diagnostic test in a non-endemic setting. | for clinical and epidemiological reasons, it is interesting to diagnose non-falciparum malaria to the species level. this retrospective study assessed the performance of the sd bioline malaria antigen pv test (fk70), a two-band immunochromatographic test detecting plasmodium vivax-specific lactate dehydrogenase, on samples of international travellers in a non-endemic setting. | 2009 | 19519915 |
| alpha-substituted norstatines as the transition-state mimic in inhibitors of multiple digestive vacuole malaria aspartic proteases. | the impact of moving the p1 side-chain from the beta-position to the alpha-position in norstatine-containing plasmepsin inhibitors was investigated, generating two new classes of tertiary alcohol-comprising alpha-benzylnorstatines and alpha-phenylnorstatines. twelve alpha-substituted norstatines were designed, synthesized and evaluated for their inhibitory potencies against plasmepsin ii and the plasmepsin iv orthologues (pm4) present in the digestive vacuole of all four plasmodium species causi ... | 2009 | 19635672 |
| implementation of a novel pcr based method for detecting malaria parasites from naturally infected mosquitoes in papua new guinea. | detection of plasmodium species in mosquitoes is important for designing vector control studies. however, most of the pcr-based detection methods show some potential limitations. the objective of this study was to introduce an effective pcr-based method for detecting plasmodium vivax and plasmodium falciparum from the field-caught mosquitoes of papua new guinea. | 2009 | 19646275 |
| longitudinal survey of malaria morbidity over 10 years in saharevo (madagascar): further lessons for strengthening malaria control. | madagascar has been known for having bio-geo-ecological diversity which is reflected by a complex malaria epidemiology ranging from hyperendemic to malaria-free areas. malaria-related attacks and infection are frequently recorded both in children and adults living in areas of low malaria transmission. to integrate this variability in the national malaria control policy, extensive epidemiological studies are required to up-date previous records and adjust strategies. | 2009 | 19660116 |
| value of liu's stain in rapid diagnosis of plasmodium ovale infection. | 2009 | 19751654 | |
| [malaria in croatia in the period between 1987 to 2006]. | a retrospective study about the incidence of malaria in croatia in the period from 1987 to 2006 based on the official data of the croatian national institute of public health. in this period there were 201 cases of malaria registered in croatia. the majority (79.6%) were imported from africa, a significantly lower number from asia (17%), and several cases from south america or from unknown locations. one case ended in death. the causative agents determined are plasmodium falciparum (64.7%), plas ... | 2009 | 19769279 |
| [imported malaria in northern central morocco, 1997-2007]. | after the elimination of autochthonous malaria in morocco in 2004, control of imported malaria, based on epidemiological monitoring of the parasite and vector control, has become a priority. this retrospective study concerns imported malaria cases identified by optical microscopy at the regional epidemiological diagnostic laboratory of the regional health department of fes from 1997 through 2007. the results obtained showed that 56 of 68 (82%) samples examined were positive. women accounted for ... | 2009 | 19801352 |
| an indigenous case of plasmodium ovale infection in sri lanka. | plasmodium ovale, which is generally prevalent only in the african region, has been emerging in the asian and southeast asian regions. it has not been reported in sri lanka. we report, to our knowledge, an indigenous case of p. ovale infection in sri lanka. this patient, who was diagnosed by a polymerase chain reaction, had no history of travel overseas or receipt of a transfusion of blood or any blood products, which makes this a likely case of indigenous transmission. this incidental finding o ... | 2008 | 18256413 |
| not all that is malaria is falciparum. | 2008 | 18291342 | |
| evaluation of fret real-time pcr assay for rapid detection and differentiation of plasmodium species in returning travellers and migrants. | a simple real-time pcr assay using one set of primer and probe for rapid, sensitive and quantitative detection of plasmodium species, with simultaneous differentiation of plasmodium falciparum from the three other plasmodium species (plasmodium vivax, plasmodium ovale and plasmodium malariae) in febrile returning travellers and migrants was developed and evaluated. | 2008 | 18442362 |
| failure of atovaquone/proguanil to prevent plasmodium ovale malaria in traveler returning from cameroon. | we report a case of a patient returning from cameroon who developed plasmodium ovale malaria, despite atovaquone/proguanil (ap, malarone) prophylaxis, which is widely used for the prevention of chloroquine-resistant malaria. ap is indeed active only on schizont blood forms of p. ovale but not against liver-stage hypnozoites and does not realize effective prophylaxis against delayed onset of p. ovale malaria. hence, this case illustrates the risk of failure with malarone for the prophylaxis of p. ... | 2008 | 18486067 |
| an 8-year survey on the occurrence of imported malaria in a nonendemic area by microscopy and molecular assays. | our study aimed to describe the occurrence of imported malaria in a nonendemic area (parma, italy) during the period 2000 to 2007, comparing the data obtained by microscopy and molecular assays targeting plasmodial 18s subunit rrna gene. the prevalence of imported malaria in parma was 21.8% by microscopy and 22.7% by polymerase chain reaction (pcr). plasmodium falciparum accounted for 81.1% of the cases, followed by plasmodium ovale (8.8%), plasmodium vivax (3.8%), and plasmodium malariae (1.9%) ... | 2008 | 18501548 |
| malaria chemoprophylaxis: strategies for risk groups. | the risk of malaria for travelers varies from region to region and depends on the intensity of transmission, the duration of the stay in the area of endemicity, the style of travel, and the efficacy of preventive measures. the decision to recommend chemoprophylaxis to travelers to areas with a low risk of malarial infection is especially difficult because the risk of infection must be balanced with the risk of experiencing side effects. if the risk of side effects by far exceeds the risk of infe ... | 2008 | 18625682 |
| identification of differentially expressed genes after acute exposure to bulaquine (cdri 80/53) in mice liver. | therapeutic agents derived from 8-aminoquinoline possess potent activity against hepatic stages of plasmodia. bulaquine (cdri 80/53), an enamine analogue of primaquine and a relatively new derivative of 8-aminioquinoline, synthesized at the central drug research institute, lucknow, india, has shown promising activity against hypnozoites of plasmodium vivax and plasmodium ovale. moreover, it has been found to be three to four times safer than primaquine in pre-clinical studies. in this study, glo ... | 2008 | 18684221 |
| malaria in refugees from tanzania--king county, washington, 2007. | recent immigrants and refugees constitute a substantial proportion of malaria cases in the united states, accounting for nearly one in 10 imported malaria cases involving persons with known resident status in 2006. this report describes three cases of plasmodium falciparum malaria and two cases of plasmodium ovale malaria that occurred during june 27-october 15, 2007 in king county, washington. the infections were diagnosed in burundian refugees who had recently arrived in the united states from ... | 2008 | 18701876 |
| [malaria diagnosis: thickdrop and bloodsmear examination, and rapid test]. | 2008 | 18711803 | |
| failure to detect plasmodium vivax in west and central africa by pcr species typing. | plasmodium vivax is estimated to affect 75 million people annually. it is reportedly absent, however, from west and central africa due to the high prevalence of the duffy negative phenotype in the indigenous populations. despite this, non-african travellers consistently return to their own countries with p. vivax malaria after visiting this region. an attempt was made, therefore, to detect the presence of p. vivax parasites in blood samples collected from the indigenous populations of west and c ... | 2008 | 18783630 |
| acute respiratory distress syndrome in a case of plasmodium ovale malaria. | acute respiratory distress syndrome is a well-known complication in plasmodium falciparum infection. it is less frequently described in plasmodium vivax, and only one case is reported in plasmodium ovale. here we present the second description of this pulmonary complication in a p. ovale acute infection. | 2008 | 18784231 |
| pcr-based detection of plasmodium in anopheles mosquitoes: a comparison of a new high-throughput assay with existing methods. | detection of the four malaria-causing plasmodium species (plasmodium falciparum, plasmodium vivax, plasmodium ovale and plasmodium malariae) within their mosquito hosts is an essential component of vector control programmes. several pcr protocols have been developed for this purpose. many of these methods, while sensitive, require multiple pcr reactions to detect and discriminate all four plasmodium species. in this study a new high-throughput assay was developed and compared with three previous ... | 2008 | 18793416 |
| evaluation and optimization of membrane feeding compared to direct feeding as an assay for infectivity. | malaria parasite infectivity to mosquitoes has been measured in a variety of ways and setting, includind direct feeds of and/or membrane feeding blood collected from randomly selected or gametocytemic volunteers. anopheles gambiae s.l is the main vector responsible of plasmodium falciparum transmission in bancoumana and represents about 90% of the laboratory findings, whereas plasmodium malariae and plasmodium ovale together represent only 10%. | 2008 | 19055715 |
| formulation, antimalarial activity and biodistribution of oral lipid nanoemulsion of primaquine. | primaquine (pq) is one of the most widely used antimalarial and is the only available drug till date to combat relapsing form of malaria especially in case of plasmodium vivax and plasmodium ovale. primaquine acts specifically on the pre-erythrocytic schizonts which are concentrated predominantly in the liver and causes relapse after multiplication. however application of pq in higher doses is limited by severe tissue toxicity including hematological and gi related side effects which are needed ... | 2008 | 17709216 |
| evaluation of the intra- and inter-specific genetic variability of plasmodium lactate dehydrogenase. | malaria diagnosis is vital to efficient control programmes and the recent advent of malaria rapid diagnostic tests (rdts) provides a reliable and simple diagnostic method. however a characterization of the efficiency of these tests and the proteins they detect is needed to maximize rdt sensitivity. | 2007 | 17961215 |
| apparent relapse of imported plasmodium ovale malaria in a pregnant woman. | a 27 week pregnant woman who had lived in bangkok, thailand, for 18 months presented to her obstetrician with a 1-week history of intermittent fever and malaise. medical history was significant for multiple episodes of malaria during her 10 years of employment in sub-saharan africa before her relocation to thailand. the initial malaria smear was negative. she returned a week later with no resolution of her symptoms, at which time she was found to have plasmodium ovale by microscopy and polymeras ... | 2007 | 17984365 |
| plasmodium malariae and plasmodium ovale--the "bashful" malaria parasites. | although plasmodium malariae was first described as an infectious disease of humans by golgi in 1886 and plasmodium ovale identified by stevens in 1922, there are still large gaps in our knowledge of the importance of these infections as causes of malaria in different parts of the world. they have traditionally been thought of as mild illnesses that are caused by rare and, in case of p. ovale, short-lived parasites. however, recent advances in sensitive pcr diagnosis are causing a re-evaluation ... | 2007 | 17459775 |
| relapsing malaria infection in an adolescent following travel to mozambique. | infection with plasmodium ovale is uncommon in travelers. we describe a case of ovale malaria in a traveler to mozambique who initially presented several weeks after completion of his trip. species identification was ultimately achieved with a pcr-based diagnostic method. | 2007 | 17574150 |
| gametocytemia and fever in human malaria infections. | we examine the charts of 408 malaria-naive neurosyphilis patients given malaria therapy at the south carolina usphs facility, with daily records encompassing at least 93% of the duration of infection, and focus on the 152 patients infected with the st. elizabeth strain of plasmodium vivax, 82 with the mclendon strain of plasmodium filciparum, 36 with the usphs strain of plasmodium malariae, and 15 with the donaldson strain of plasmodium ovale in whom gametocytes appeared before drug, or other, i ... | 2007 | 17626355 |
| primaquine-induced differential gene expression analysis in mice liver using dna microarrays. | primaquine (pq), a clinically important derivative of 8-aminoquinoline used against the hepatic stages (hypnozoites) of plasmodium vivax and plasmodium ovale, was studied to evaluate and compare between mrna expression, and biochemical and histological parameters of hepatic stress in adult swiss mice (mus musculus). following single oral dose of pq (40 mg/kg, bw), alanine aminotransferase (alt) and aspartate aminotransferase (ast) along with hematoxylin and eosin stained liver sections did not s ... | 2007 | 17686563 |
| uk malaria treatment guidelines. | malaria is the tropical disease most commonly imported into the uk, with 1500-2000 cases reported each year, and 10-20 deaths. approximately three-quarters of reported malaria cases in the uk are caused by plasmodium falciparum, which is capable of invading a high proportion of red blood cells and rapidly leading to severe or life-threatening multi-organ disease. most non-falciparum malaria cases are caused by plasmodium vivax; a few cases are caused by the other two species of plasmodium: plasm ... | 2007 | 17215045 |
| malaria and pregnancy: the perspective in pakistan. | to study the effects of malaria on pregnancy outcome. | 2007 | 17319413 |
| genetic polymorphisms influence plasmodium ovale pcr detection accuracy. | detection of plasmodium ovale by use of a nested pcr assay with a novel plasmodium ovale primer set was superior to detection of plasmodium ovale by real-time pcr assays. nested pcr was also better at detecting p. malariae. the detection of p. ovale in many patients first admitted >2 months following their return to italy indicated that p. ovale relapses are common. | 2007 | 17360843 |
| the diagnosis of malaria and identification of plasmodium species by polymerase chain reaction in turkey. | more than half of the world's population is exposed to malaria in approximately 100 countries. rapid diagnosis and correct treatment of cases are the main objectives of control programs in malaria endemic areas. we have developed a pcr method to determine the presence of plasmodium dna in blood. the method can also identify the species of the plasmodium by restriction enzyme analysis of the amplified product. we evaluated the performance of this method in the diagnosis of malaria suspected cases ... | 2007 | 17434795 |
| evaluation of a real-time pcr assay for malaria diagnosis in patients from vietnam and in returned travellers. | real-time pcr diagnosis of malaria has advantages over traditional microscopic methods, especially when parasitaemia is low and when dealing with mixed infections. we have developed a new real-time pcr with specific genes in each plasmodium species present only in one copy to identify the four pathogenic plasmodium spp. for humans. the sensitivity was less than 25 parasites/microl. no cross-hybridisation was observed with human dna or among the four plasmodium spp. using lightcycler pcr and conv ... | 2007 | 17150235 |
| recent patent reviews on small molecule-based antimalarial drugs. | malaria is the number one disease in the world responsible for 1-3 million deaths each year. the world wide number of malaria patients is estimated at 400 to 900 million. approximately one third of the world's population lives in malaria-endemic areas, including central and south america, asia, and africa. plasmodium falciparum, plasmodium vivax, plasmodium ovale and plasmodium malariae are malaria parasites responsible for infecting humans. mosquitoes that carry malaria parasites have become re ... | 2007 | 18221180 |
| stronger host response per parasitized erythrocyte in plasmodium vivax or ovale than in plasmodium falciparum malaria. | fever tends to start at a lower level of parasitemia in plasmodium vivax or ovale than in p. falciparum malaria, but hyperparasitemia and complications are more likely to occur in p. falciparum malaria. therefore, we compared the relationship between parasitemia and host response parameters before therapy in 97 patients with p. faciparum malaria (18 with complications), and 28 with p. vivax or ovale malaria. | 2006 | 16772003 |
| moderate transmission but high prevalence of malaria in madagascar. | malaria transmission remains poorly documented in areas of low transmission. a study has been carried out over two consecutive years in analamiranga, a village located at an altitude of 885m on the western edge of the malagasy highlands, with the aim of generating and updating malariometric indexes for both mosquitoes and schoolchildren. in this village, no vector control measures were performed during the study period nor during previous decades. mosquitoes were collected monthly when landing o ... | 2006 | 16842796 |
| detection and identification of human plasmodium species with real-time quantitative nucleic acid sequence-based amplification. | decisions concerning malaria treatment depend on species identification causing disease. microscopy is most frequently used, but at low parasitaemia (<20 parasites/mul) the technique becomes less sensitive and time consuming. rapid diagnostic tests based on plasmodium antigen detection do often not allow for species discrimination as microscopy does, but also become insensitive at <100 parasites/microl. | 2006 | 17018138 |
| [severe imported malaria. the experience of the military hospital of marrakech]. | incidence of severe imported malaria increases with the multiplication of humanitarian and military missions in malarial endemic areas. the purpose of this study was to describe the demographic, clinical, therapeutic and outcome aspects of 9 cases which have been hospitalized in the intensive care unit and medecine service of the military hospital of marrakech, between january 2001 and december 2004. out of 68 patients admitted with symptomatic malaria during this period, 9 cases were considered ... | 2006 | 17040884 |
| a case of imported plasmodium ovale malaria. | there have been reports in korea of imported malaria cases of four plasmodium species, but there has been no report of imported plasmodium ovale malaria confirmed by molecular biological methods. we report an imported case of that was confirmed by wright-giemsa-stained peripheral blood smear and nested polymerase chain reaction targeting the small subunit ribosomal rna gene. the amplified dna was sequenced and compared with other registered p. ovale isolates. the isolate in this study was a memb ... | 2006 | 17043432 |
| tertian malaria (plasmodium vivax and plasmodium ovale) in two travelers despite atovaquone-proguanil prophylaxis. | there is limited data regarding the efficacy of prophylaxis with atovaquone/proguanil (a/p) against non-falciparum malaria in travelers. two cases, one plasmodium vivax infection and another plasmodium ovale infection, in travelers despite a/p prophylaxis are presented. | 2006 | 17107431 |
| space-time clustering of childhood malaria at the household level: a dynamic cohort in a mali village. | spatial and temporal heterogeneities in the risk of malaria have led the who to recommend fine-scale stratification of the epidemiological situation, making it possible to set up actions and clinical or basic researches targeting high-risk zones. before initiating such studies it is necessary to define local patterns of malaria transmission and infection (in time and in space) in order to facilitate selection of the appropriate study population and the intervention allocation. the aim of this st ... | 2006 | 17118176 |
| patterns of imported malaria at the academic medical center, amsterdam, the netherlands. | in the netherlands, cases of imported malaria peaked in the late 1990s to around 500 (60% plasmodium falciparum) annually. about 30% to 40% of all cases and 57% to 69% of the falciparum cases presented in the academic medical center, amsterdam. in 1991 to 1994, a shift in population groups to more semi-immune patients, mostly settled immigrants visiting friends and relatives (vfrs), was noticed, when compared to 1979 to 1988. this study shows the ongoing trend in 2000 to 2002. | 2006 | 16412103 |
| real-time polymerase chain reaction assay for the rapid detection and characterization of chloroquine-resistant plasmodium falciparum malaria in returned travelers. | imported drug-resistant malaria is a growing problem in industrialized countries. rapid and accurate diagnosis is essential to prevent malaria-associated mortality in returned travelers. however, outside of a limited number of specialized centers, the microscopic diagnosis of malaria is slow, unreliable, and provides little information about drug resistance. molecular diagnostics have the potential to overcome these limitations. | 2006 | 16447106 |
| [malaria. primary symptoms: fever, headache, nausea, vomiting, diarrhea]. | 2006 | 16523989 | |
| low autochtonous urban malaria in antananarivo (madagascar). | the study of urban malaria is an area undergoing rapid expansion, after many years of neglect. the problem of over-diagnosis of malaria, especially in low transmission settings including urban areas, is also receiving deserved attention. the primary objective of the present study was to assess the frequency of malaria among febrile outpatients seen in private and public primary care facilities of antananarivo. the second aim was to determine, among the diagnosed malaria cases, the contribution o ... | 2006 | 16573843 |
| [severe imported malaria: the experience of the avicenna military hospital of marrakech]. | incidence of severe imported malaria increases with the multiplication of humanitarian and military missions in malarial endemic areas. the purpose of this study was to describe the demographic, clinical, therapeutic and outcome aspects of 9 cases which have been hospitalized in the intensive care unit and medecine service of the military hospital of marrakech, between january 2001 and december 2004. out of 68 patients admitted with symptomatic malaria during this period, 9 cases were considered ... | 2006 | 17446159 |
| comparison of pcr and microscopy for the detection of asymptomatic malaria in a plasmodium falciparum/vivax endemic area in thailand. | the main objective of this study was to compare the performance of nested pcr with expert microscopy as a means of detecting plasmodium parasites during active malaria surveillance in western thailand. | 2006 | 17169142 |
| minor liver profile dysfunctions in plasmodium vivax, p. malaria and p. ovale patients and normalization after treatment. | liver function tests were performed in 61 vivax, 54 malariae and 15 ovale malaria patients who were admitted to bangkok hospital for tropical diseases between 2001 and 2004. the objective of the study was to evaluate changes in hepatic biochemical indices before and after treatment with artemisinin derivatives. on admission and prior to treatment, hepatic dysfunction was found among the 3 groups. serum liver function tests and physical examinations were performed weekly during the 28-day follow- ... | 2006 | 17170571 |
| plasmodium ovale--a case report from gujarat. | 2006 | 17175709 | |
| outpatient treatment of malaria in recently arrived african migrants. | to describe the clinical features and management of african migrants recently arrived in western australia and subsequently diagnosed with malaria. | 2006 | 17181500 |
| epidemiology of malaria in new halfa, an irrigated area in eastern sudan. | a prospective study investigated the epidemiology of malaria in an agricultural area in eastern sudan from november 1999 to june 2000 (1 irrigation and 1 dry season). in monthly parasite surveys, 99/ 1539 blood films from 190 individuals were positive for malaria: 95% for plasmodium falciparum, 3% p. vivax and 2% p. ovale. the slide positive rate (spr) of malaria episodes ranged from 3.7% to 12.8% in different months of the survey, with a peak in january. there was no significant difference in s ... | 2005 | 16602473 |
| [an imported case of mixed malaria caused by plasmodium falciparum and plasmodium ovale.] | malaria caused by plasmodium species is an important parasitic infection in turkey as in the rest of the world. malaria cases originating in our country are caused by p. vivax; those caused by other plasmodium spp. are imported cases. in this article, after work-related travel to cameroon, a patient who acquired specific clinical signs and symptoms of malaria has been evaluated. the major clinical findings of the patient were fever, chills and shaking. after examination of thin and thick blood s ... | 2005 | 17160826 |
| failure of primaquine therapy for the treatment of plasmodium ovale malaria. | 2005 | 16231275 | |
| evaluation of a real-time polymerase chain reaction assay for the diagnosis of malaria in patients from thailand. | we compared the diagnosis of malaria in 297 patients from thailand by a real-time polymerase chain reaction (pcr) assay using the lightcycler with conventional microscopy using giemsa-stained thick and thin blood films. the pcr assay can be completed in one hour and has the potential to detect and identify four species of plasmodium in a single reaction by use of melting temperature curve analysis (however, we did not detect plasmodium ovale in this study). blood was collected, stored, and trans ... | 2005 | 16282292 |
| late relapse of plasmodium ovale malaria--philadelphia, pennsylvania, november 2004. | approximately 1,300 cases of malaria are reported each year in the united states; nearly all of these cases occur in travelers, many of whom fail to receive or adhere to prescribed chemoprophylaxis or do not follow recommendations for prevention of mosquito bites. malaria can persist if not treated or if treated incorrectly (e.g., with an ineffective drug or an incorrect dosage of an effective drug). early treatment is required to avoid severe illness or death. although malaria typically becomes ... | 2005 | 16340939 |
| malaria "epidemic" in quebec: diagnosis and response to imported malaria. | imported malaria is an increasing problem. the arrival of 224 african refugees presented the opportunity to investigate the diagnosis and management of imported malaria within the quebec health care system. | 2005 | 15632404 |
| [malaria in the emergency room. results of the emergency treatment of 137 patients with symptomatic malaria]. | to assess characteristics and outcome of emergency patients with acute malaria. | 2005 | 15662580 |
| ineffectiveness of the binax now malaria test for diagnosis of plasmodium ovale malaria. | 2005 | 15695736 | |
| active-site specificity of digestive aspartic peptidases from the four species of plasmodium that infect humans using chromogenic combinatorial peptide libraries. | two targeted chromogenic octapeptide combinatorial libraries, comprised of 38 pools each containing 361 different peptides, were used to analyze the enzyme/substrate interactions of five plasmepsins. the first library (p1 library) was based on a good synthetic aspartic peptidase substrate [westling, j., cipullo, p., hung, s. h., saft, h., dame, j. b., and dunn, b. m. (1999) protein sci. 8, 2001-2009; scarborough, p. e., and dunn, b. m. (1994) protein eng. 7, 495-502] and had the sequence lys-pro ... | 2005 | 15697202 |
| plasmodium (haemamoeba) cathemerium gene sequences for phylogenetic analysis of malaria parasites. | the dna sequence information on avian malaria parasites of the genus plasmodium is quite limited. at present, sequences of only 6 out of 34 valid species are available. however, sequence data of avian malaria parasites are particularly important with regard to the resolution of the phylogenetic relationships of the most virulent human malaria agent, plasmodium falciparum. the question as to whether p. falciparum originates from avian or from mammalian parasites would contribute to our understand ... | 2005 | 15812672 |
| glucose-6-phosphate dehydrogenase deficiency in two returning operation iraqi freedom soldiers who developed hemolytic anemia while receiving primaquine prophylaxis for malaria. | use of antimalarial prophylaxis continues to be routine practice among military personnel returning from areas where malaria is endemic. primaquine may be used for terminal prophylaxis against plasmodium ovale and plasmodium vivax. serious complications of this regimen are infrequent. we report the occurrence of significant hemolytic anemia for two soldiers returning from operation iraqi freedom. they presented with dark urine, headaches, and classic laboratory findings of hemolysis. both soldie ... | 2005 | 15916292 |
| large sequence heterogeneity of the small subunit ribosomal rna gene of plasmodium ovale in cambodia. | plasmodium ovale malaria has been reported in various countries in southeast asia, but never in cambodia. using a species-specific polymerase chain reaction (pcr) targeting the small subunit (ssu) ribosomal rna (rrna) gene, we detected p. ovale in nearly 4% of the inhabitants of a northeastern cambodian village. plasmodium ovale was associated with at least one other plasmodium species, and two quadruple infections were detected. the diagnosis was confirmed by microscopy and by ssu rrna pcr prod ... | 2005 | 15964956 |
| factors influencing the pattern of imported malaria. | data on imported malaria in industrialized areas are known to be incomplete because of underreporting and lack of homogeneity. these facts and the complexity of factors influencing the transmission of malaria render their interpretation difficult. the relevance of various factors is usually not fully considered, although their impact on recommendations for chemoprophylaxis may be important. | 2005 | 15996451 |
| plasmodium ovale: parasite and disease. | humans are infected by four recognized species of malaria parasites. the last of these to be recognized and described is plasmodium ovale. like the other malaria parasites of primates, this parasite is only transmitted via the bites of infected anopheles mosquitoes. the prepatent period in the human ranges from 12 to 20 days. some forms in the liver have delayed development, and relapse may occur after periods of up to 4 years after infection. the developmental cycle in the blood lasts approxima ... | 2005 | 16020691 |
| plasmodium species mixed infections in two areas of manhiça district, mozambique. | we compared the distribution patterns of individual plasmodium species and mixed-species infections in two geographically close endemic areas, but showing environmental differences. comparisons concerned circulating plasmodium infections in both human and mosquito vector populations in the dry and wet seasons, at a micro-epidemiological level (households). both areas revealed a very high overall prevalence of infection, all year-round and in all age groups. plasmodium falciparum was the predomin ... | 2005 | 16094461 |
| targeting primaquine into liver using chylomicron emulsions for potential vivax malaria therapy. | primaquine (pq) exerts a broad spectrum of activities against various stages of parasitic malaria. it remains as the only drug that destroys late hepatic stages and latent tissue forms of plasmodium vivax and plasmodium ovale. however, systems that can target pq to liver hepatocytes, where malarial sporozoites reside, are needed to minimize the dose-limiting severe toxicities and side-effects caused by pq. recently, a reconstituted artificial chylomicron emulsion was generated using commercially ... | 2005 | 16140485 |
| development of a real-time pcr assay for detection of plasmodium falciparum, plasmodium vivax, and plasmodium ovale for routine clinical diagnosis. | a taqman-based real-time pcr qualitative assay for the detection of three species of malaria parasites-plasmodium falciparum, p. ovale, and p. vivax-was devised and evaluated using 122 whole-blood samples from patients who had traveled to areas where malaria is endemic and who presented with malaria-like symptoms and fever. the assay was compared to conventional microscopy and to an established nested-pcr assay. the specificity of the new assay was confirmed by sequencing the pcr products from a ... | 2004 | 15004078 |
| compensatory evolution in the human malaria parasite plasmodium ovale. | the fixation of neutral compensatory mutations in a population depends on the effective population size of the species, which can fluctuate dramatically within a few generations, the mutation rate, and the selection intensity associated with the individual mutations. we observe compensatory mutations and intermediate states in populations of the malaria parasite plasmodium ovale. the appearance of compensatory mutations and intermediate states in p. ovale raises interesting questions about popul ... | 2004 | 15020451 |
| evaluation of a new plate hybridization assay for the laboratory diagnosis of imported malaria in italy. | a new molecular diagnostic method "malaria-ibridogen" (amplimedical s.p.a.--bioline division, turin, italy) based on a plate-hybridization assay for the simultaneous detection and identification of human malaria parasites was evaluated in this study. a target dna sequence of the plasmodial 18s ribosomal rna gene was amplified by polymerase chain reaction (pcr) and hybridized in microtiter wells with five biotinylated probes each specific for plasmodium falciparum, p. vivax, p. malariae, p. ovale ... | 2004 | 15164627 |
| recombinant expression and enzymatic subsite characterization of plasmepsin 4 from the four plasmodium species infecting man. | plasmepsin 4 from plasmodium falciparum and orthologs from plasmodium malariae, plasmodium ovale and plasmodium vivax have been expressed in recombinant form, and properties of the active site of each enzyme characterized by kinetic analysis. a panel of chromogenic peptide substrates systematically substituted at the p3, p2, p2' and p3' positions was used to estimate enzyme/ligand interactions in the corresponding enzyme subsites based upon kinetic data. the kinetic parameters kcat, km and kcat/ ... | 2004 | 15287591 |
| [characteristics of malaria cases diagnosed in edirne province between 1994-2002]. | in this study, the epidemiological characteristics of malaria cases in edirne province were investigated. between the years of 1994-2002, a total of 317,087 blood samples were collected from soldiers in the province with selective active surveillance and from the resident population with active or passive surveillance methods, by the medical staff of malaria control department and health centers, to search the presence of plasmodium. in 281 of them plasmodium spp. were detected, and the characte ... | 2004 | 15293910 |
| molecular analysis of plasmodium ovale variants. | complete dna sequences of the small subunit ribosomal rna (ssurrna) gene and partial sequences of three other loci were obtained from three variant-type and three classic-type plasmodium ovale isolates from southeast asia and compared with genbank-available data. three different ssurrna sequences (pov 1-3) were found in each variant-type isolate, and two different ssurrna sequences (poc 1-2) in each classic-type isolate. pov 1-3 were closer to sequences previously found in the cameroon and mal/m ... | 2004 | 15324543 |
| case report: plasmodium ovale infection acquired in malawi. | malaria due to plasmodium ovale is uncommon outside west africa. a 37-year-old male german who had returned from malawi four months previously presented in september 2003 because of fever recurring every two days. the patient had never been to west africa. microscopy of stained thick and thin blood films revealed p. ovale. this is the first report of a p. ovale infection acquired in malawi, east africa. malaria surveillance centres should monitor the possible emergence of autochtonous transmissi ... | 2004 | 15485706 |