Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| pterocarpenes elicited by aspergillus caelatus in peanut (arachis hypogaea) seeds. | the substituted pterocarpenes named aracarpene-1 (1) and aracarpene-2 (2) were isolated from wounded peanut seeds challenged by a strain of aspergillus caelatus. the structures of these putative phytoalexins were determined by interpretation of nmr and ms data. the aracarpenes were investigated for their antifungal and antibacterial activities as well as antioxidant, anti-inflammatory, and cytotoxic activities in mammalian cells. aracarpene-2 demonstrated high antibacterial properties against te ... | 2010 | 21030054 |
| production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker. | the use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. mat (multi-auto-transformation) vector system has been one of the different strategies to excise the selection marker gene and produce marker-free transgenic plants. in the present study, ipt (isopentenyl transferase) gene was used as a selection marker gene. ... | 2010 | 21184230 |
| the arabidopsis botrytis susceptible1 interactor defines a subclass of ring e3 ligases that regulate pathogen and stress responses. | we studied the function of arabidopsis (arabidopsis thaliana) botrytis susceptible1 interactor (boi) in plant responses to pathogen infection and abiotic stress. boi physically interacts with and ubiquitinates arabidopsis bos1, an r2r3myb transcription factor previously implicated in stress and pathogen responses. in transgenic plants expressing the bos1-β-glucuronidase transgene, β-glucuronidase activity could be detected only after inhibition of the proteosome, suggesting that bos1 is a target ... | 2010 | 20921156 |
| [isolation and identification of an endophytic fungus of polygonatum cyrtonema and its antifungal metabolites]. | endophytic fungi in medicinal plants produce a variety of bioactivity compounds. in this study, an endophytic fungus zjqy610 with antifungal activity was isolated from polygonatum cyrtonema in zhejiang qingyuan baishanzu mountain nature reserve. | 2010 | 20931871 |
| multiple resistance of botrytis cinerea from kiwifruit to sdhis, qois and fungicides of other chemical groups. | botrytis cinerea pers.: fr. is a high-risk pathogen for fungicide resistance development that has caused resistance problems on many crops throughout the world. this study investigated the fungicide sensitivity profile of isolates from kiwifruits originating from three greek locations with different fungicide use histories. sensitivity was measured by in vitro fungitoxicity tests on artificial nutrient media. | 2010 | 20730988 |
| sensitivity of botrytis cinerea to chitosan and acibenzolar-s-methyl. | the antifungal properties of chitosan and acibenzolar-s-methyl were evaluated to assess their potential for protecting grapes against botrytis cinerea pers.: fr. isolated from vitis vinifera l. the objectives were to determine the effects of these compounds on the in vitro development of b. cinerea and to assess their effectiveness at controlling grey mould on grapes stored at different temperatures. | 2010 | 20730989 |
| gold nanoparticle-assisted single base-pair mismatch discrimination on a microfluidic microarray device. | two simple gold nanoparticle (gnp)-based dna analysis methods using a microfluidic device are presented. in the first method, probe dna molecules are immobilized on the surface of a self-assembled submonolayer of gnps. the hybridization efficiency of the target oligonulceotides was improved due to nanoscale spacing between probe molecules. in the second method, target dna molecules, oligonulceotides or polymerase chain reaction (pcr) amplicons, are first bound to gnps and then hybridized to the ... | 2010 | 21045930 |
| characterization and pathogenicity of botryosphaeriaceae species collected from olive and other hosts in spain and california. | species in the family botryosphaeriaceae are common pathogens causing fruit rot and dieback of many woody plants. in this study, 150 botryosphaeriaceae isolates were collected from olive and other hosts in spain and california. representative isolates of each type were characterized based on morphological features and comparisons of dna sequence data of three regions: internal transcribed spacer 5.8s, β-tubulin, and elongation factor. three main species were identified as neofusicoccum mediterra ... | 2010 | 20731532 |
| arabidopsis histone methyltransferase set domain group8 mediates induction of the jasmonate/ethylene pathway genes in plant defense response to necrotrophic fungi. | as sessile organisms, plants have to endure a wide variety of biotic and abiotic stresses, and accordingly they have evolved intricate and rapidly inducible defense strategies associated with the activation of a battery of genes. among other mechanisms, changes in chromatin structure are thought to provide a flexible, global, and stable means for the regulation of gene transcription. in support of this idea, we demonstrate here that the arabidopsis (arabidopsis thaliana) histone methyltransferas ... | 2010 | 20810545 |
| involvement of lipoxygenase in elicitor-stimulated sanguinarine accumulation in papaver somniferum suspension cultures. | the involvement of lipoxygenase (lox, ec 1.13.11.12) in elicitor-induced opium poppy defense response was investigated. papaver somniferum l. suspension cultures were treated with abiotic elicitor methyl jasmonate (mj), fungal elicitor (botrytis cinerea homogenate) and phenidone (specific inhibitor of lox) to determine the involvement of this enzyme in production of sanguinarine, the major secondary metabolite of opium poppy cultures. p. somniferum suspension cultures responded to elicitor treat ... | 2010 | 20829053 |
| isolation and identification of 5-hydroxyl-5-methyl-2-hexenoic acid from actinoplanes sp. hbdn08 with antifungal activity. | a bioactivity-guided approach was employed to isolate and determine the chemical identity of bioactive constituents with antifungal activity from actinoplanes sp. hbdn08. the structure of the antifungal metabolite was elucidated as 5-hydroxyl-5-methyl-2-hexenoic acid on the basis of spectral analysis. this compound showed strong in vitro antifungal activity against botrytis cinerea, cladosporium cucumerinum and corynespora cassiicola, with an ic(50) of 32.45, 27.17, and 30.66 mg/l, respectively; ... | 2010 | 20584599 |
| detection of botrytis cinerea by loop-mediated isothermal amplification. | to develop a sensitive, rapid and simple method for detection of botrytis cinerea based on loop-mediated isothermal amplification (lamp) that would be suitable for use outside a conventional laboratory setting. | 2010 | 21029140 |
| induction of systemic resistance in plants by biochar, a soil-applied carbon sequestering agent. | biochar is the solid coproduct of biomass pyrolysis, a technique used for carbon-negative production of second-generation biofuels. the biochar can be applied as a soil amendment, where it permanently sequesters carbon from the atmosphere as well as improves soil tilth, nutrient retention, and crop productivity. in addition to its other benefits in soil, we found that soil-applied biochar induces systemic resistance to the foliar fungal pathogens botrytis cinerea (gray mold) and leveillula tauri ... | 2010 | 20701489 |
| hypersensitivity pneumonitis due to molds in a saxophone player. | this 48-year-old patient was evaluated for an interstitial pneumonia. an open-lung biopsy showed a pattern of nonspecific interstitial pneumonia. the ct scan appearance, showing mosaic ground-glass opacities in the ventilated parts of the lung, the centrolobular predominance of inflammation on the lung sections, and the presence of a lymphocytic alveolitis at bal suggested a hypersensitivity pneumonitis. the patient was a white-collar worker and had no contact with pets, birds, drugs, or molds a ... | 2010 | 20822994 |
| hypoxylon sp., an endophyte of persea indica, producing 1,8-cineole and other bioactive volatiles with fuel potential. | an endophytic fungus of persea indica was identified, on the basis of its anamorphic stage, as nodulosporium sp. by sem. partial sequence analysis of its rdna revealed the identity of the teleomorphic stage of the fungus as hypoxylon sp. it produces an impressive spectrum of volatile organic compounds (vocs), most notably 1,8-cineole, 1-methyl-1,4-cyclohexadiene, and tentatively identified (+)-.alpha.-methylene-.alpha.-fenchocamphorone, among many others, most of which are unidentified. six-day- ... | 2010 | 20953951 |
| in vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mould disease agent botrytis cinerea. | the aim of this study was to find an alternative to synthetic fungicides currently used in the control of devastating fungal pathogen botrytis cinerea, the causal agent of grey mould disease of tomato. antifungal activities of essential oils obtained from aerial parts of aromatic plants, which belong to the lamiacea family such as origanum (origanum syriacum l. var. bevanii), lavender (lavandula stoechas l. var. stoechas) and rosemary (rosmarinus officinalis l.), were investigated against b. cin ... | 2010 | 20826038 |
| diverse accumulation of several dehydrin-like proteins in cauliflower (brassica oleracea var. botrytis), arabidopsis thaliana and yellow lupin (lupinus luteus) mitochondria under cold and heat stress. | dehydrins represent hydrophilic proteins acting mainly during cell dehydration and stress response. dehydrins are generally thermostable; however, the so-called dehydrin-like (dehydrin-related) proteins show variable thermolability. both groups immunoreact with antibodies directed against the k-segment of dehydrins. plant mitochondrial dehydrin-like proteins are poorly characterized. the purpose of this study was to extend previous reports on plant dehydrins by comparing the level of immunopreci ... | 2010 | 20718974 |
| role of nitric oxide and reactive oxide species in disease resistance to necrotrophic pathogens. | nitric oxide (no) and reactive oxygen species (ros) are important signaling molecules in plant immunity. however, roles of no and ros in disease resistance to necrotrophic pathogens are not fully understood. we have recently demonstrated that no plays a pivotal role in basal defense against botrytis cinerea and the expression of the salicylic acid (sa) -responsive gene pr-1in nicotiana benthamiana. by contrast, ros function negatively in resistance or positively in expansion of disease lesions d ... | 2010 | 20448455 |
| the arabidopsis p450 protein cyp82c2 modulates jasmonate-induced root growth inhibition, defense gene expression and indole glucosinolate biosynthesis. | jasmonic acid (ja) is a fatty acid-derived signaling molecule that regulates a broad range of plant defense responses against herbivores and some microbial pathogens. molecular genetic studies have established that ja also performs a critical role in several aspects of plant development. here, we describe the characterization of the arabidopsis mutant jasmonic acid-hypersensitive1-1 (jah1-1), which is defective in several aspects of ja responses. although the mutant exhibits increased sensitivit ... | 2010 | 20354503 |
| inhibitory activity of tea polyphenol and hanseniaspora uvarum against botrytis cinerea infections. | to investigate the effect of tea polyphenol (tp) and hanseniaspora uvarum alone or in combination against botrytis cinerea in grapes and to evaluate the possible mechanisms involved. | 2010 | 20633212 |
| biocatalytic preparation and absolute configuration of enantiomerically pure fungistatic anti-2-benzylindane derivatives. study of the detoxification mechanism by botrytis cinerea. | enantiomerically pure 2-benzylindane derivatives were prepared using biocatalytic methods and their absolute configuration determined. (1r,2s)-2-benzylindan-1-ol ((1r,2s)-2) and (s)-2-benzylindan-1-one ((s)-3) were produced by fermenting baker's yeast. lipase-mediated esterifications and hydrolysis of the corresponding racemic substrates gave rise to the enantiopure compounds (1s,2r)-2-benzylindan-1-ol ((1s,2r)-2) and (1r,2s)-2-benzylindan-1-ol ((1r,2s)-2), respectively. the antifungal activity ... | 2010 | 20574563 |
| inadvertent gene silencing of argininosuccinate synthase (bcass1) in botrytis cinerea by the plob1 vector system. | for several years, researchers working on the plant pathogen botrytis cinerea and a number of other related fungi have routinely used the plob1 vector system, based on hygromycin resistance, under the control of the aspergillus nidulans olic promoter and what was reported to be the beta-tubulin (tuba) terminator. recently, it has been demonstrated that this vector contains a 446-bp portion of the b. cinerea argininosuccinate synthase gene (bcass1) rather than the tuba terminator. as argininosucc ... | 2010 | 20696000 |
| postharvest management of gray mold and brown rot on surfaces of peaches and grapes using electrolyzed oxidizing water. | this study evaluated the potential use of near-neutral (ph=6.3-6.5) electrolyzed oxidizing water (eo water) to inactivate pure cultures of botrytis cinerea and monilinia fructicola and to mitigate fungal infection of these organisms on fruit surfaces. treatment of these organisms, in pure culture, with eo water at concentrations of 25, 50, 75, and 100ppm total residual chlorine (trc) and 10 min of contact time resulted in a 6 log(10) spores/ml reduction of both organisms. a dip treatment or a di ... | 2010 | 20696490 |
| potent cryoprotective activity of cold and co2-regulated cherimoya (annona cherimola) endochitinase. | a cryoprotective chitinase (bchi14) was isolated and purified from the mesocarp of co(2)-treated cherimoya fruit (annona cherimola mill.) stored at chilling temperature by anion exchange and chromatofocusing chromatography. this hydrolase was characterized as an endochitinase with a m(r) of 14.31 kda and a pi of 8.26, belonging to the family 19 of glycosyl hydrolases (gh19). while it was stable over a wide ph range and active in a broad acidic ph range, it had an optimum ph of 7.0. its optimum t ... | 2010 | 20576315 |
| trichome patterning in arabidopsis thaliana from genetic to molecular models. | the aerial organs of plants typically produce trichomes that may adopt various functions, including light, wind, frost, and herbivore protection. trichomes are of epidermal origin regularly distributed on the surface. the mechanism by which trichome differentiation is triggered in individual cells in a field of protodermal cells is best studied in arabidopsis thaliana. the genetic analysis has revealed a number of key genes controlling this patterning process, and further molecular analysis has ... | 2010 | 20705186 |
| pesticides' influence on wine fermentation. | wine quality strongly depends on the grape quality. to obtain high-quality wines, it is necessary to process healthy grapes at the correct ripeness stage and for this reason the farmer has to be especially careful in the prevention of parasite attacks on the grapevine. the most common fungal diseases affecting grape quality are downy and powdery mildew (plasmopara viticola and uncinula necator), and gray mold (botrytis cinerea). on the other hand, the most dangerous insects are the grape moth (l ... | 2010 | 20610173 |
| development of resistance against blackleg disease in brassica oleracea var. botrytis through in silico methods. | blackleg caused by leptosphaeria maculans is a very important disease worldwide and causes large yield loss of significant vegetable crops of brassicaceae family. absence of resistant 'b' genome in vegetable brassica oleracea proposed these species susceptible to blackleg disease. we present a specific piece of molecular modelling work, combining in silico docking methods, energy minimization calculations and in silico cloning, to develop a rational peptide as a candidate that functions as a res ... | 2010 | 20619351 |
| uv-b impact on aphid performance mediated by plant quality and plant changes induced by aphids. | plants face various abiotic and biotic environmental factors and therefore need to adjust their phenotypic traits on several levels. uv-b radiation is believed to impact herbivorous insects via host plant changes. plant responses to abiotic challenges (uv-b radiation) and their interaction with two aphid species were explored in a multifactor approach. broccoli plants [brassica oleracea l. convar. botrytis (l.), brassicaceae] were grown in two differently covered greenhouses, transmitting either ... | 2010 | 20636911 |
| microextraction and gas chromatography/mass spectrometry for improved analysis of geosmin and other fungal "off" volatiles in grape juice. | geosmin is a volatile fungal metabolite with an earthy aroma produced in grape products from rotten grapes. the accumulation of geosmin in grapes is caused by the interaction of botrytis cinerea and penicillium expansum. solid phase microextraction (spme) has great utility for collecting volatile compounds in wine. however, contamination with earthy odours may have occurred previously in the must and novel methods are required for this commodity. in the present report, several parameters of the ... | 2010 | 20655340 |
| validation of a predictive model for the growth of botrytis cinerea and penicillium expansum on grape berries. | the objective of this study was to develop and to validate a model for predicting the combined effect of temperature and a(w) on the radial growth rate, mu, of botrytis cinerea and penicillium expansum on grape berries. the proposed strategy was based on the gamma-concept developed previously [zwietering, m.h., wijtzes, t., de wit, j.c., van't riet, k. 1992. a decision support system for prediction of the microbial spoilage in foods. journal of food protection. 12, 973-979]: mu=mu(opt).gamma(t). ... | 2010 | 20619474 |
| characters of aerated compost tea from immature compost that limit colonization of bean leaflets by botrytis cinerea. | the aim was to produce and characterize an aerated compost tea (act) that suppressed growth of the plant pathogen botrytis cinerea. | 2010 | 20629795 |
| mapk phosphatase mkp2 mediates disease responses in arabidopsis and functionally interacts with mpk3 and mpk6. | mitogen-activated protein kinase (mapk) cascades have important functions in plant stress responses and development and are key players in reactive oxygen species (ros) signalling and in innate immunity. in arabidopsis, the transmission of ros and pathogen signalling by mapks involves the coordinated activation of mpk6 and mpk3; however, the specificity of their negative regulation by phosphatases is not fully known. here, we present genetic analyses showing that mapk phosphatase 2 (mkp2) regula ... | 2010 | 20626661 |
| antifungal activity of glyceollins isolated from soybean elicited with aspergillus sojae. | the aim of this study was to examine the efficacy of glyceollins isolated from soybean seeds elicited with aspergillus sojae for its antifungal potential. glyceollins i, ii, and iii were determined by hplc-ms analysis. the glyceollins (200 and 600 microg/disk) revealed remarkable antifungal effect against fusarium oxysporum , phytophthora capsici, sclerotinia sclerotiorum, and botrytis cinerea, within the growth inhibition range of 10.9-61.0%, along with their respective mic values ranging from ... | 2010 | 20666365 |
| disruption of the bcchs3a chitin synthase gene in botrytis cinerea is responsible for altered adhesion and overstimulation of host plant immunity. | the fungal cell wall is a dynamic structure that protects the cell from different environmental stresses suggesting that wall synthesizing enzymes are of great importance for fungal virulence. previously, we reported the isolation and characterization of a mutant in class iii chitin synthase, bcchs3a, in the phytopathogenic fungus botrytis cinerea. we demonstrated that virulence of this mutant is severely impaired. here, we describe the virulence phenotype of the cell-wall mutant bcchs3a on the ... | 2010 | 20672878 |
| genome characterization of a debilitation-associated mitovirus infecting the phytopathogenic fungus botrytis cinerea. | the full-length sequences of botrytiscinereamitovirus 1 (bcmv1) and an associated rna (bcmv1-s) in strain canbc-1c-78 of botrytis cinerea were determined. sequence analysis showed that bcmv1 is 2804 nt long and au-rich (66.8%). bcmv1 shares 95% nucleotide sequence identity with ophiostomanovo-ulmimitovirus 3b (onumv3b). however, it is 472 nt longer than onumv3b. mitochondrial codon usage revealed that bcmv1 contains one open reading frame encoding rdrp, which is 96% identical to the rdrp of onum ... | 2010 | 20674953 |
| synthesis, fungicidal activity, and structure-activity relationship of 2-oxo- and 2-hydroxycycloalkylsulfonamides. | to explore new potential fungicides, a series of novel compounds, including 11 2-oxocycloalkylsulfonamide (3) and 21 2-hydroxycycloalkylsulfonamide (4) derivatives, were synthesized and their structures were confirmed by (1)h nuclear magnetic resonance (nmr), infrared (ir), and elemental analysis. the results of the bioassay showed that the compounds 3 and 4 possessed excellent fungicidal activity against botrytis cinerea pers. both in vitro and in vivo. the fungicidal activity of the compounds ... | 2010 | 20929233 |
| distinct defenses induced in wheat against powdery mildew by acetylated and nonacetylated oligogalacturonides. | in wheat, little is known about disease resistance inducers and, more specifically, about the biological activities from those derived from endogenous elicitors, such as oligogalacturonides (ogas). therefore, we tested the ability of two fractions of ogas, with polymerization degrees (dps) of 2-25, to induce resistance to blumeria graminis f. sp. tritici and defense responses in wheat. one fraction was unacetylated (ogas-ac) whereas the second one was 30% chemically acetylated (ogas+ac). infecti ... | 2010 | 20684658 |
| exploring mechanisms of resistance to respiratory inhibitors in field strains of botrytis cinerea, the causal agent of gray mold. | respiratory inhibitors are among the fungicides most widely used for disease control on crops. most are strobilurins and carboxamides, inhibiting the cytochrome b of mitochondrial complex iii and the succinate dehydrogenase of mitochondrial complex ii, respectively. a few years after the approval of these inhibitors for use on grapevines, field isolates of botrytis cinerea, the causal agent of gray mold, resistant to one or both of these classes of fungicide were recovered in france and germany. ... | 2010 | 20693447 |
| deficiencies in jasmonate-mediated plant defense reveal quantitative variation in botrytis cinerea pathogenesis. | despite the described central role of jasmonate signaling in plant defense against necrotrophic pathogens, the existence of intraspecific variation in pathogen capacity to activate or evade plant jasmonate-mediated defenses is rarely considered. experimental infection of jasmonate-deficient and jasmonate-insensitive arabidopsis thaliana with diverse isolates of the necrotrophic fungal pathogen botrytis cinerea revealed pathogen variation for virulence inhibition by jasmonate-mediated plant defen ... | 2010 | 20419157 |
| enhancement of biocontrol efficacy of rhodotorula glutinis by salicyclic acid against gray mold spoilage of strawberries. | the control activity of rhodotorulaglutinis, salicylic acid (sa), alone or in combination, on gray mold spoilage and natural spoilage of strawberries was investigated. r. glutinis as stand-alone treatment, and the combined treatment of sa at 100 microg/ml with r. glutinis significantly reduced the disease incidence and lesion diameter of gray mold spoilage, respectively at both 20 degrees c and 4 degrees c. after 20 d storage at 4 degrees c, the combination of sa and r. glutinis was more effecti ... | 2010 | 20488570 |
| a domain swap approach reveals a role of the plant wall-associated kinase 1 (wak1) as a receptor of oligogalacturonides. | oligogalacturonides (ogs) released from the plant cell wall are active both as damage-associated molecular patterns (damps) for the activation of the plant immune response and regulators of plant growth and development. members of the wall-associated kinase (wak) family are candidate receptors of ogs, due to their ability to bind in vitro these oligosaccharides. because lethality and redundancy have hampered the study of waks by reverse genetics, we have adopted a chimeric receptor approach to e ... | 2010 | 20439716 |
| the mitogen-activated protein kinase kinase bos5 is involved in regulating vegetative differentiation and virulence in botrytis cinerea. | we present a characterization of bos5 from botrytis cinerea, a gene that encodes a mitogen-activated protein kinase kinase (mapkk), which is homologous to os-5 of neurospora crassa. the bos5 gene deletion mutant exhibited reduced vegetative growth and strongly impaired conidiation. the mutant also exhibited increased sensitivity to the dicarboximide fungicide iprodione and to osmotic stress mediated by nacl or kcl. western-blot analysis showed that the bcsak1 protein, the putative downstream com ... | 2010 | 20595070 |
| evidence that core histone h3 is targeted to the mitochondria in brassica oleracea. | the core histone proteins h2a, h2b, h3 (histone h3) and h4 are known to form nucleosomes with nuclear dna, but are historically considered to be absent from mitochondria. we suggest that h3 is a dual-targeted protein, found in mitochondria as well as n (nuclei). wolf psort and mitoprot analyses of h3 sequences revealed mitochondrial targeting signals, and immunohistochemistry indicated mitochondrial distribution. western blots of brassica oleracea cv. botrytis (cauliflower) mitochondrial extract ... | 2010 | 20597863 |
| a novel endophytic huperzine a-producing fungus, shiraia sp. slf14, isolated from huperzia serrata. | to characterize and identify a novel huperzine a (hupa)-producing fungal strain slf14 isolated from huperzia serrata (thunb. ex murray) trev. in china. | 2010 | 20602655 |
| a role for beta-sitosterol to stigmasterol conversion in plant-pathogen interactions. | upon inoculation with pathogenic microbes, plants induce an array of metabolic changes that potentially contribute to induced resistance or even enhance susceptibility. when analysing leaf lipid composition during the arabidopsis thaliana-pseudomonas syringae interaction, we found that accumulation of the phytosterol stigmasterol is a significant plant metabolic process that occurs upon bacterial leaf infection. stigmasterol is synthesized from beta-sitosterol by the cytochrome p450 cyp710a1 via ... | 2010 | 20444228 |
| antimicrobial activity of confertifolin from polygonum hydropiper. | confertifolin (6,6,9a-trimethyl-4,5,5a,6,7,8,9,9a-octahydronaphtho[1,2-c] furan-3 (1h)-one) was isolated from the essential oil of polygonum hydropiper l. (polygonaceae) leaves using column chromatography. confertifolin showed activity both in bacteria and fungi. the lowest mic for bacteria was observed against enterococcus faecalis (31.25 microg/ml). significant mic for fungi was observed against scopulariopsis sp (7.81 microg/ml), curvularia lunata (7.81 microg/ml), epidermophyton floccosum (7 ... | 2010 | 20645838 |
| [the effect of different endophytic fungi on chrysanthemum morifolium output and quality]. | to enhance the flowers yield of chrysanthemum morifolium by endophytic fungi. | 2010 | 20518295 |
| two new furanoeremophilanes from senecio santelisis. | from an argentine collection of senecio santelisis phil., the new furanoeremophilanoids, (10betah)-6beta-acetoxy-1alpha-hydroxyfuranoeremophilan-9-one (1) and (10betah)-1alpha-hydroxy-6beta-(propanoyloxy)furanoeremophilan-9-one (2), together with the known (10alphah)-6beta-acetoxy-1alpha-hydroxyfuranoeremophilan-9-one (3), (10alphah)-1alpha,6beta-diacetoxyfuranoeremophilan-9-one (4), and (10alphah)-1alpha-hydroxy-6beta-(propanoyloxy)furanoeremophilan-9-one (5) were isolated. their structures and ... | 2010 | 20658662 |
| mitogen-activated protein kinase 3 and 6 regulate botrytis cinerea-induced ethylene production in arabidopsis. | plants challenged by pathogens, especially necrotrophic fungi such as botrytis cinerea, produce high levels of ethylene. at present, the signaling pathways underlying the induction of ethylene after pathogen infection are largely unknown. mpk6, an arabidopsis stress-responsive mitogen-activated protein kinase (mapk) was previously shown to regulate the stability of acs2 and acs6, two type i acs isozymes (1-amino-cyclopropane-1-carboxylic acid synthase). phosphorylation of acs2 and acs6 by mpk6 p ... | 2010 | 20659280 |
| arabidopsis thaliana plant defensin atpdf1.1 is involved in the plant response to biotic stress. | *previously, it was shown that the arabidopsis thaliana plant defensins atpdf1.1 (at1g75830) and atpdf1.2a (at5g44420) exert in vitro antimicrobial properties and that their corresponding genes are expressed in seeds and induced in leaves upon pathogen attack, respectively. *in this study, the expression profile of both atpdf1.1 and atpdf1.2a is analysed in wild-type plants upon different stress-related treatments and the effect of modulation of their expression in transgenic plants is examined ... | 2010 | 20561213 |
| the botrytis cinerea early secretome. | the extracellular proteome, or secretome, of phytopathogenic fungi is presumed to be a key element of their infection strategy. especially interesting constituents of this set are those proteins secreted at the beginning of the infection, during the germination of conidia on the plant surfaces or wounds, since they may play essential roles in the establishment of a successful infection. we have germinated botrytis cinerea conidia in conditions that resemble the plant environment, a synthetic med ... | 2010 | 20564262 |
| a copper-transporting atpase bcccc2 is necessary for pathogenicity of botrytis cinerea. | copper is an essential trace element that serves as a cofactor for numerous enzymes. in eukaryotes, copper-transporting atpases deliver copper to various copper-containing proteins in the trans-golgi network. this study identified a copper-transporting atpase gene bcccc2 in a fungus pathogenic to plants, botrytis cinerea. we investigated the biological roles of bcccc2 by generating null mutants for bcccc2. melanization, conidiation and the formation of sclerotia were severely affected in deltabc ... | 2010 | 20526618 |
| the flavohemoglobin bcfhg1 is the main no detoxification system and confers protection against nitrosative conditions but is not a virulence factor in the fungal necrotroph botrytis cinerea. | flavohemoglobins constitute a group of proteins involved in the metabolism of nitric oxide (no). botrytis cinerea was shown to have a single flavohemoglobin coding gene, bcfhg1. its expression was developmentally regulated, with maximum expression levels during germination of conidia, and was enhanced very quickly upon exposure to no of germinating conidia, but not of mycelium growing and branching actively. expression in planta paralleled the expression pattern during saprophytic growth with ma ... | 2010 | 20223291 |
| effects of heat treatment on wound healing in gala and red fuji apple fruits. | this study investigated the effects of heat treatment (hot air at 38 degrees c for 4 days) on wound healing in gala and red fuji apple fruits (malus domestica borkh.) and the possible mechanism. wounded apples were healed at either 20 or 38 degrees c for 4 days. during the treatment, ethylene, phenylalanine ammonia-lyase (pal), peroxidase (pod), polyphenol oxidase (ppo), hydrogen peroxide (h(2)o(2)), and phenolic and lignin contents were measured. following the treatment, healed wounds were inoc ... | 2010 | 20232915 |
| genetic characterization of grapevine-infecting botrytis cinerea isolates from argentina. | botrytis cinerea is an ascomycete with a high genetic diversity and complex population structure, as reported from several hosts and sites. however, nothing is known about its genetic diversity in argentina. | 2010 | 20346300 |
| analysis of fungal gene expression by real time quantitative pcr. | the real-time quantitative pcr (qpcr) method has become central for the quantification of gene expression as well as other applications. the major advantages of qpcr are the utilization of small amount of template, high sensitivity and the ability to detect products during the reaction. after selecting qpcr among other options (northern blot, semi-quantitative pcr), one should consider several factors. the first and critical step in qpcr of fungi is the selection of an appropriate growth medium ... | 2010 | 20238263 |
| synthesis and fungicidal activity of aryl carbamic acid-5-aryl-2-furanmethyl ester. | chitin, a major structural component of insect cuticle and fungus cell wall but absent in plants and vertebrates, is regarded as a safe and selective target for pest control agents. chitin synthesis inhibitors (csis) have been well-known as insect growth regulators (igrs) but rarely found as fungicides in agriculture. to find novel csis with good activity, benzoylphenylurea, a typical kind of csis, was chosen as the lead compound and 26 novel aryl carbamic acid-5-aryl-2-furanmethyl esters were d ... | 2010 | 20151651 |
| contamination by moulds of grape berries in slovakia. | this paper describes the first map, albeit partial, of toxigenic fungi re-isolated from grape berries collected in three out of the six most important slovakia winemaking areas in two different periods of the harvest year 2008. low temperatures and high relative humidity during july 2008 favoured the development of grape fungal diseases that cause rots such as plasmopara, uncinula, botrytis, metasphaeria, elsinoë, and saccharomycetes. in the analysed samples, the following genera of toxigenic fu ... | 2010 | 20349371 |
| oligomycins a and c, major secondary metabolites isolated from the newly isolated strain streptomyces diastaticus. | during the screening program for fungicides, one actinomycete strain eco 00047 was isolated with the potential activity against fungus. according to the morphology and analysis of the nucleotide sequence of the 16s rrna gene (1500 bp) this isolate was identified as streptomyces diastaticus. the active compounds were separated by silica gel column chromatography, sephadex lh-20 gel filtration and then purified by flash chromatography on c18 (20-45 microm). the chemical structure of the bioactive ... | 2010 | 20336498 |
| wounding-induced wrky8 is involved in basal defense in arabidopsis. | the wrky family of plant transcription factors controls several types of plant stress responses. arabidopsis wrky8, localized to the nucleus, is mainly induced by abscissic acid, h(2)o(2), wounding, pseudomonas syringae and botrytis cinerea infection, and aphid and maggot feeding. to determine its biological functions, we isolated loss-of-function t-dna insertion mutants and generated gain-of-function overexpressing wrky8 transgenic plants in arabidopsis. plants expressing the mutated wrky8 gene ... | 2010 | 20367464 |
| transcriptional responses to polycyclic aromatic hydrocarbon-induced stress in arabidopsis thaliana reveal the involvement of hormone and defense signaling pathways. | polycyclic aromatic hydrocarbons (pahs) are toxic, widely-distributed, environmentally persistent, and carcinogenic byproducts of carbon-based fuel combustion. previously, plant studies have shown that pahs induce oxidative stress, reduce growth, and cause leaf deformation as well as tissue necrosis. to understand the transcriptional changes that occur during these processes, we performed microarray experiments on arabidopsis thaliana l. under phenanthrene treatment, and compared the results to ... | 2010 | 20377843 |
| polygalacturonase inhibiting protein: isolation, developmental regulation and pathogen related expression in panax ginseng c.a. meyer. | polygalacturonase inhibiting proteins (pgips) are the major defense proteins which play an important role in resistance to infection of pathogens. a putative novel gene encoding pgip was isolated from panax ginseng c.a. meyer, which shows 70.3 and 68.4% homology with chick pea and arabidopsis pgips. the race pcr was preformed to isolate the full-length pgip cdna from panax ginseng. sequence analysis revealed that the cdna of pgpgip is of 1,275 bp in length and that it's containing orf encodes fo ... | 2010 | 19946753 |
| local lesions and induced resistance. | the local lesion phenomenon is one of the most notable resistance mechanisms where virus after multiplying in several hundred cells around the point of entry, does not continue to spread and remains in a local infection. several types of local lesions are known, inter alia, necrotic, chlorotic, and starch lesions. cells inside the lesion generally contain much less virus than cells in a systemic infection. cytopathic changes accompany the local lesion development. proteases that may have propert ... | 2010 | 20109664 |
| disruption of poly(adp-ribosyl)ation mechanisms alters responses of arabidopsis to biotic stress. | poly(adp-ribosyl)ation is a posttranslational protein modification in which adp-ribose (adp-rib) units derived from nad(+) are attached to proteins by poly(adp-rib) polymerase (parp) enzymes. adp-rib groups are removed from these polymer chains by the enzyme poly(adp-rib) glycohydrolase (parg). in animals, poly(adp-ribosyl)ation is associated with dna damage responses and programmed cell death. previously, we hypothesized a role for poly(adp-ribosyl)ation in plant defense responses when we detec ... | 2010 | 19889874 |
| the botrytis cinerea xylanase xyn11a contributes to virulence with its necrotizing activity, not with its catalytic activity. | the botrytis cinerea xylanase xyn11a has been previously shown to be required for full virulence of this organism despite its poor contribution to the secreted xylanase activity and the low xylan content of b. cinerea hosts. intriguingly, xylanases from other fungi have been shown to have the property, independent of the xylan degrading activity, to induce necrosis when applied to plant tissues, so we decided to test the hypothesis that secreted xyn11a contributes to virulence by promoting the n ... | 2010 | 20184750 |
| synthesis and biological activity of organotin 4-methyl-1,2,3-thiadiazole-5-carboxylates and benzo[1,2,3]thiadiazole-7-carboxylates. | a series of organotin 4-methyl-1,2,3-thiadiazole-5-carboxylates and benzo[1,2,3]thiadiazole-7-carboxylates have been synthesized and characterized by nmr ((1)h, (13)c, and (119)sn), ir, and elemental analyses. the structure of the dimeric complex {[(bthco(2))snet(2)](2)o}(2) (bth represents benzo[1,2,3]thiadiazol-7-yl) has been further confirmed by x-ray diffraction crystallography. assessment for fungicidal activity indicates that all of the newly synthesized compounds exhibit good growth inhib ... | 2010 | 20014765 |
| novel bifunctional nucleases, ombbd and atbbd1, are involved in abscisic acid-mediated callose deposition in arabidopsis. | screening of the expressed sequence tag library of the wild rice species oryza minuta revealed an unknown gene that was rapidly and strongly induced in response to attack by a rice fungal pathogen (magnaporthe oryzae) and an insect (nilaparvata lugens) and by wounding, abscisic acid (aba), and methyl jasmonate treatments. its recombinant protein was identified as a bifunctional nuclease with both rnase and dnase activities in vitro. this gene was designated ombbd (for o. minuta bifunctional nucl ... | 2010 | 20018603 |
| tryptophan-derived metabolites are required for antifungal defense in the arabidopsis mlo2 mutant. | arabidopsis (arabidopsis thaliana) genes mildew resistance locus o2 (mlo2), mlo6, and mlo12 exhibit unequal genetic redundancy with respect to the modulation of defense responses against powdery mildew fungi and the control of developmental phenotypes such as premature leaf decay. we show that early chlorosis and necrosis of rosette leaves in mlo2 mlo6 mlo12 mutants reflects an authentic but untimely leaf senescence program. comparative transcriptional profiling revealed that transcripts of seve ... | 2010 | 20023151 |
| determination of trans- and cis-resveratrol in serbian commercial wines. | resveratrol (trans-3,5,4'-trihydroxystilbene) is a phytoalexin produced by grapevines in response to fungal infection, particularly to botrytis cinerea. it has been shown that it possess various biological effects such as prevention of cardiovascular diseases and anti-inflammatory and anticancerogenic properties. red wines are a primary source of resveratrol. although a number of investigations have focused on the determination of resveratrol in wines of different countries, there is no similar ... | 2010 | 20223091 |
| inhibitory effect of boron against botrytis cinerea on table grapes and its possible mechanisms of action. | boron, an essential plant micronutrient, was effective in the form of potassium tetraborate for control of postharvest gray mold caused by botrytis cinerea on table grapes stored at room temperature or at 0 degrees c. the inhibition of fruit decay was closely correlated with boron concentrations and partially influenced by ph value of the solution. boron strongly inhibited spore germination, germ tube elongation, and mycelial spread of b. cinerea in the culture medium. application of boron at 1% ... | 2010 | 20060611 |
| synthesis, fungicidal activity, and structure-activity relationship of spiro-compounds containing macrolactam (macrolactone) and thiadiazoline rings. | two series of novel spiro-compounds containing macrolactam or macrolactone and thiadiazoline rings, 1-thia-2-alkylimino-3,4,9-triaza-10-oxospiro[4.15]eicosyl-3-ene (4f) and 1-thia-2-alkylimino-3,4-diaza-9-oxa-10-oxospiro[4.15]eicosyl-3-ene (4g), were synthesized from 12-oxo-1,15-pentadecanlactam and 12-oxo-1,15-pentadecanlactone, respectively. their structures were confirmed by elemental analysis, (1)h nmr, and (13)c nmr. the conformation of compounds 4f was determined via the crystal structure ... | 2010 | 20041703 |
| effect of culture media and ph on the biomass production and biocontrol efficacy of a metschnikowia pulcherrima strain to be used as a biofungicide for postharvest disease control. | few strains of metschnikowia pulcherrima (pitt) m.w. miller are under development for control of postharvest pathogens on fruit. a substrate was developed to optimize the biomass production of m. pulcherrima strain bio126. different complex nutrient sources, with or without ph control, were tested. growth in yeast extract provided at concentrations > or =30 g*l-1 yielded the highest biomass. the addition of two carbon sources, d-mannitol and l-sorbose, at 5 g*l-1 each, significantly improved yea ... | 2010 | 20237574 |
| ethylene signaling renders the jasmonate response of arabidopsis insensitive to future suppression by salicylic acid. | cross-talk between jasmonate (ja), ethylene (et), and salicylic acid (sa) signaling is thought to operate as a mechanism to fine-tune induced defenses that are activated in response to multiple attackers. here, 43 arabidopsis genotypes impaired in hormone signaling or defense-related processes were screened for their ability to express sa-mediated suppression of ja-responsive gene expression. mutant cev1, which displays constitutive expression of ja and et responses, appeared to be insensitive t ... | 2010 | 20064062 |
| chitinolytic and antifungal activity of a bacillus pumilus chitinase expressed in arabidopsis. | the bacillus pumilus sg2 chitinase gene (chis) and its truncated form lacking chitin binding (chbd) and fibronectin type iii (fniii) domains were transformed to arabidopsis plants and the expression, functionality and antifungal activity of the recombinant proteins were investigated. results showed that while the two enzyme forms showed almost equal hydrolytic activity toward colloidal chitin, they exhibited a significant difference in antifungal activity. recombinant chis in plant protein extra ... | 2010 | 20035370 |
| insights into the mechanisms of toxicity and tolerance to the agricultural fungicide mancozeb in yeast, as suggested by a chemogenomic approach. | abstract saccharomyces cerevisiae was used to uncover the mechanisms underlying tolerance and toxicity of the agricultural fungicide mancozeb, linked to cancer and parkinson's disease development. chemogenomics screening of a yeast deletion mutant collection revealed 286 genes that provide protection against mancozeb toxicity. the most significant gene ontology (go) terms enriched in this dataset are associated to transcriptional machinery, vacuolar organization and biogenesis, intracellular tra ... | 2010 | 20337531 |
| tomato plants transformed with the inhibitor-of-virus-replication gene are partially resistant to botrytis cinerea. | tomato plants transformed with a cdna clone encoding the inhibitor-of-virus-replication (ivr) gene were partially resistant to botrytis cinerea. this resistance was observed as a significant reduction in the size of lesions induced by the fungus in transgenic plants compared with the lesions on the nontransgenic control plants. this resistance was weakened when plants were kept at an elevated temperature, 32 degrees c, before inoculation with b. cinerea compared with plants kept at 17 to 22 degr ... | 2010 | 20128695 |
| novel insights into mannitol metabolism in the fungal plant pathogen botrytis cinerea. | in order to redefine the mannitol pathway in the necrotrophic plant pathogen botrytis cinerea, we used a targeted deletion strategy of genes encoding two proteins of mannitol metabolism, bcmtdh (b. cinerea mannitol dehydrogenase) and bcmpd (b. cinerea mannitol-1-phosphate dehydrogenase). mobilization of mannitol and quantification of bcmpd and bcmtdh gene transcripts during development and osmotic stress confirmed a role for mannitol as a temporary and disposable carbon storage compound. in orde ... | 2010 | 20136633 |
| the role of mitogen-activated protein (map) kinase signalling components and the ste12 transcription factor in germination and pathogenicity of botrytis cinerea. | in all fungi studied so far, mitogen-activated protein (map) kinase cascades serve as central signalling complexes that are involved in various aspects of growth, stress response and infection. in this work, putative components of the yeast fus3/kss1-type map kinase cascade and the putative downstream transcription factor ste12 were analysed in the grey mould fungus botrytis cinerea. deletion mutants of the map triple kinase ste11, the map kinase kinase ste7 and the map kinase adaptor protein st ... | 2010 | 20078780 |
| ulocladium cantlous sp. nov. isolated from northwestern china: its morphology and molecular phylogenetic position. | a new species of ulocladium was isolated from diseased leaves from two cucumis sp. growing in sinkiang and gansu provinces of china. conidia were isolated from necrotic leaves and used to establish single-spore pure cultures. conidia were harvested from cultures 7 d after incubation for morphological comparisons. the morphology of this species resembles that of u. botrytis and u. consortiale. however it is distinguished from these two species by the sizes of obovoid to broadly ellipsoidal conidi ... | 2010 | 20361504 |
| optimization of a microfluidic microarray device for the fast discrimination of fungal pathogenic dna. | a microfluidic microarray device, which has been developed for parallel dna detection, is now further optimized for more rapid and sensitive dna detection and for the single-base-pair discrimination of two fungal pathogenic pcr products. two poly(dimethylsiloxane) (pdms)-based microfluidic chips consist of radial and spiral microchannels in which flexible probe creation and convenient sample delivery have been achieved by centrifugal pumping. the microarray hybridizations occurred at the cross s ... | 2010 | 20083083 |
| 2-de proteomic approach to the botrytis cinerea secretome induced with different carbon sources and plant-based elicitors. | botrytis cinerea is a phytopathogenic fungus infecting a number of crops (tomatoes, grapes and strawberries), which has been adopted as a model system in molecular phytopathology. b. cinerea uses a wide variety of infection strategies, which are mediated by a set of genes/proteins called pathogenicity/virulence factors. many of these factors have been described as secreted proteins, and thus the study of this sub-proteome, the secretome, under changing circumstances can help us to understand the ... | 2010 | 20376862 |
| burkholdines 1097 and 1229, potent antifungal peptides from burkholderia ambifaria 2.2n. | potent antifungal cyclic lipopeptides, burkholdines (bk), were isolated from a culture of burkholderia ambifaria 2.2n. bk-1229 (1) and bk-1097 (2) are octapeptides comprised of nonproteinogenic amino acids, including beta-hydroxytyrosine, beta-hydroxyasparagine, and a new fatty acyl amino acid. 1 and 2 are fungicidal against a panel of fungi with potencies 2-60-fold better than amphotericin b control. | 2010 | 20085289 |
| [construction of t-dna insertion mutants of botrytis cinerea via agrobacterium tumefaciens mediated transformation and sequence analysis of insertion site]. | construction of botrytis cinerea t-dna insertion mutant library through agrobacterium-mediated transformation and for further understanding of the molecular mechanisms underlying the pathogenicity of botrytis cinerea. | 2010 | 20387458 |
| geobacillus sp., a thermophilic soil bacterium producing volatile antibiotics. | geobacillus, a bacterial genus, is represented by over 25 species of gram-positive isolates from various man-made and natural thermophilic areas around the world. an isolate of this genus (m-7) has been acquired from a thermal area near yellowstone national park, mt and partially characterized. the cells of this organism are globose (ca. 0.5 mu diameter), and they are covered in a matrix capsule which gives rise to elongate multicelled bacilliform structures (ranging from 3 to 12 mum) as seen by ... | 2010 | 20091406 |
| sexual mating of botrytis cinerea illustrates prp8 intein heg activity. | strains of botrytis cinerea are polymorphic for the presence of an intein in the prp8 gene (intein +/-). the intein encodes a homing endonuclease (heg). during meiosis in an intein +/- heterozygote, the homing endonuclease initiates intein 'homing' by inducing gene conversion. in such meioses, the homing endonuclease triggers gene conversion of the intein together with its flanking sequences into the empty allele. the efficiency of gene conversion of the intein was found to be 100%. the extent o ... | 2010 | 20093192 |
| synthesis of 1-acyl-3-isopropenylbenzimidazolone derivatives and their activity against botrytis cinerea. | a series of 1-acyl-3-isopropenylbenzimidazolone derivatives were synthesized, and their structures were characterized by (1)h and (13)c nmr and elemental analysis. their fungicidal activities against botrytis cinerea were also evaluated by spore germination assay. the acrylic acid, methacrylic acid, 4-chlorophenyl acetic acid, and 2-chlorobenzoic acid derivatives exhibited strong fungicidal activity. this implied that benzimidazolones might be potential fungicide leading compounds. | 2010 | 20102200 |
| a transcriptomic study of grapevine (vitis vinifera cv. cabernet-sauvignon) interaction with the vascular ascomycete fungus eutypa lata. | eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. in the present work, microarrays were made in order (i) to improve our knowledge of grapevine (vitis vinifera cv. cabernet-sauvignon) responses to eutypa lata, the causal agent of eutypa dieback; and (ii) to identify genes that may prevent symptom development. qiagen/operon grapevine microarrays comprising 14,500 probes were used to compare, under three experimental conditions (in vitro, in the greenhou ... | 2010 | 20190040 |
| aromatic potential of botrytized white wine grapes: identification and quantification of new cysteine-s-conjugate flavor precursors. | sweet wines made from botrytized grapes contain much higher concentrations of volatile thiols, especially 3-sulfanylhexan-1-ol (3sh), than dry white wines. three new specific volatile thiols (3-sulfanylpentan-1-ol (3sp), 3-sulfanylheptan-1-ol (3shp), and 2-methyl-3-sulfanylbutan-1-ol (2m3sb) were recently identified in sauternes wines. like most volatile thiols, these compounds were almost totally absent from must, mainly being formed during alcoholic fermentation. in this work, we describe the ... | 2010 | 20103162 |
| transfer of the beta-tubulin gene of botrytis cinerea with resistance to carbendazim into fusarium graminearum. | resistance to carbendazim and other benzimidazole fungicides in botrytis cinerea (pers. ex fr.) and most other fungi is usually conferred by mutation(s) in a single chromosomal beta-tubulin gene, often with several allelic mutations. in fusarium graminearum schwade, however, carbendazim resistance is not associated with a mutation in the corresponding beta-tubulin gene. | 2010 | 20063268 |
| synthesis of nh006--a photostable fungicide effective against botrytis cinerea--according to the asymmetric total synthesis of mk8383. | mk8383, isolated from phoma sp. t2526 in 1993, exhibits potent antibiotic activities against a variety of phytopathogens and has been considered a promising fungicide against botrytis cinerea. unfortunately, mk8383 is a photosensitive compound and it undergoes irreversible decomposition. although much effort has been devoted to improving the photostability of mk8383 by chemical modification of its structure by a research group organized by meiji seika kaishya, ltd. and mitsubishi chemical corpor ... | 2010 | 20449485 |
| antifungal activities of an endophytic pseudomonas fluorescens strain pf1tz harbouring genes from pyoluteorin and phenazine clusters. | pseudomonas fluorescens pf1tz was inhibitory in vitro to a number of phytopathogenic fungi and could protect vine plantlets against botrytis cinerea. total protection was reached after 3 weeks of bacterial inoculation. the endophytism of pf1tz was confirmed by confocal microscopy using its inherent fluorescence. the molecular characterization of pf1tz indicated the presence of genes from clusters encoding pyoluteorin and phenazine. the chromatographic purification and lc-ms(n) analysis revealed ... | 2010 | 20458521 |
| outdoor allergenic fungal spores: comparison between an urban and a rural area in northern portugal. | the frequency and concentration of many airborne fungal spores associated with respiratory allergy symptoms are influenced by geographical and climatic characteristics. | 2010 | 20461966 |
| purification of novel protein elicitor from botrytis cinerea that induces disease resistance and drought tolerance in plants. | pebc1, a novel protein elicitor was isolated and purified from the mycelium of gray mold fungus, botrytis cinerea strain bc-4-2-2-1. the protein was eluted through hitrap deae ff and resource q anion exchange chromatography and displayed as a single band with an apparent molecular weight of 36 kda on silver staining sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the pi of the purified protein pebc1 was determined by 2-de and was 4.85. three peptide segments were obtained b ... | 2010 | 19616421 |
| arabidopsis thaliana class-ii tga transcription factors are essential activators of jasmonic acid/ethylene-induced defense responses. | the three closely related arabidopsis basic leucine zipper (bzip) transcription factors tga2, tga5 and tga6 are required for the establishment of the salicylic acid (sa)-dependent plant defense response systemic acquired resistance, which is effective against biotrophic pathogens. here we show that the same transcription factors are essential for the activation of jasmonic acid (ja)- and ethylene (et)-dependent defense mechanisms that counteract necrotrophic pathogens: the tga256 triple mutant i ... | 2010 | 19832945 |
| pr-1, a novel antifungal protein from pumpkin rinds. | a novel antifungal protein, m(r) = ca. 40 kda, was isolated from pumpkin rind and designated pr-1. when purified by anion exchange chromatography and hplc, it inhibited growth of several fungi including botrytis cinerea, fusarium oxysporum, fusarium solani and rhizoctonia solani, as well as the yeast, candida albicans, at 10-20 microm. it did not inhibit growth of escherichia coli or staphylococcus aureus even at 200 microm. laser scanning microscopy of fungal cells exposed to rhodamine-labeled ... | 2010 | 19760117 |
| a combination of heat treatment and pichia guilliermondii prevents cherry tomato spoilage by fungi. | this study investigated the effectiveness of heat treatment and pichia guilliermondii, either alone or in combination, to combat postharvest fungal spoilage in cherry tomato fruit. in vitro experiments demonstrated that heat treatment at 38 degrees c significantly inhibited mycelial growth of three different pathogens (botrytis cinerea, alternaria alternata and rhizopus stolonifer ehrenb). in vivo experiments unveiled that either heat treatment or p. guilliermondii reduced decay caused by these ... | 2010 | 19923029 |
| the nature of tobacco resistance against botrytis cinerea depends on the infection structures of the pathogen. | to protect themselves, plants have evolved an armoury of defences in response to pathogens and other stress situations. these include the production of pathogenesis-related (pr) proteins and the accumulation of antimicrobial molecules such as phytoalexins. here we report that resistance of tobacco to botrytis cinerea is cultivar specific. nicotiana tabacum cv. petit havana but not n. tabacum cv. xanthi or cv. samsun is resistant to b. cinerea. this resistance is correlated with the accumulation ... | 2010 | 19799622 |
| pseudomonas spp.-induced systemic resistance to botrytis cinerea is associated with induction and priming of defence responses in grapevine. | non-pathogenic rhizobacteria pseudomonas spp. can reduce disease in plant tissues through induction of a defence state known as induced systemic resistance (isr). this resistance is based on multiple bacterial determinants, but nothing is known about the mechanisms underlying rhizobacteria-induced resistance in grapevine. in this study, the ability of pseudomonas fluorescens cha0 and pseudomonas aeruginosa 7nsk2 to induce resistance in grapevine against botrytis cinerea is demonstrated. both str ... | 2010 | 19812243 |
| the botrytis cinerea aspartic proteinase family. | the ascomycete plant pathogen botrytis cinerea secretes aspartic proteinase (ap) activity. functional analysis was carried out on five aspartic proteinase genes (bcap1-5) reported previously. single and double mutants lacking these five genes showed neither a reduced secreted proteolytic activity, nor a reduction in virulence and they showed no alteration in sensitivity to antifungal proteins purified from grape juice. scrutiny of the b. cinerea genome revealed the presence of nine additional bc ... | 2010 | 19853057 |
| a storage protein-like trypsin inhibitor from the moth bean (phaseolus acutifolius) with antiproliferative activity toward lymphoma cells. | a 26-kda trypsin inhibitor with an n-terminal sequence resembling storage proteins was purified from moth beans (phaseolus acutifolius). it dose-dependently inhibited trypsin with an ic(50) value of about 0.38 microm. it inhibited [methyl-(3)h] thymidine incorporation by lymphoma mbl2 cells with an ic(50) value of 20 microm. | 2010 | 20044920 |