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coaggregation profiles of the microflora from root surface caries lesions.bacterial coaggregation reactions between different species and the auto aggregation of the same species are associated with the initiation and development of dental plaque and biofilms. as no such data is available on isolates from root caries lesions, we evaluated, by a visual, semi-quantitative scoring system and a spectrophotometric, quantitative assay, the coaggregation of 22 different wild-type microbial species comprising ten bacterial genera and a single candida spp. the quantitative coa ...200515598414
prosthetic valve endocarditis caused by veillonella parvula.veillonella species is a rare cause of endocarditis. we report a case of a 49-year-old man with veillonella parvula prosthetic valve endocarditis who presented with acute cardiac failure due to valvular dehiscence. his clinical course was complicated by cortical blindness and limb paresis as a result of cerebral embolism. the endocarditis was successfully treated with urgent valve replacement surgery and a prolonged course of metronidazole.200515603847
differences in the subgingival microbiota of swedish and usa subjects who were periodontally healthy or exhibited minimal periodontal disease.previous studies have shown differences in the mean proportions of subgingival species in samples from periodontitis subjects in different countries, which may relate to differences in diet, genetics, disease susceptibility and manifestation. the purpose of the present investigation was to determine whether there were differences in the subgingival microbiotas of swedish and american subjects who exhibited periodontal health or minimal periodontal disease.200515642056
analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time pcr.the gut microbiota may contribute to the onset and maintenance of irritable bowel syndrome (ibs). in this study, the microbiotas of patients suffering from ibs were compared with a control group devoid of gastrointestinal (gi) symptoms.200515667495
evaluation of bacterial nitrate reduction in the human oral cavity.it is hypothesized that the enterosalivary nitrate circulation encourages nitrate reducing bacteria to reside within the oral cavity. nitrite production may then limit the growth of acidogenic bacteria as a result of the production of antimicrobial oxides of nitrogen, including nitric oxide. this study was carried out with 10 subjects to characterize oral nitrate reduction and identify the bacteria responsible. nitrate reduction varied between individuals (mean 85.4 +/- 15.9 nmol nitrite min(-1) ...200515693824
influence of antimicrobial treatment for helicobacter pylori infection on the intestinal microflora in japanese macaques.eradication treatment for helicobacter pylori is known to cause mild but relatively frequent adverse effects. some adverse effects such as diarrhoea and soft stools are related to disruption of the composition of the intestinal microflora. this study investigated the microfloral changes resulting from administration of an eradication regimen using proton pump inhibitor (ppi), amoxycillin and clarithromycin. twenty-eight laboratory-bred japanese macaques either were administered eradication treat ...200515713617
microbiological culture analysis of the tongue anaerobic microflora in subjects with and without halitosis.determination of the microflora present on the tongue dorsum of subjects with and without halitosis using conventional microbiological culture methods.200515752102
scaling and root planing, systemic metronidazole and professional plaque removal in the treatment of chronic periodontitis in a brazilian population ii--microbiological results.the current investigation evaluated changes in levels and proportions of 39 bacterial species in subgingival plaque samples after scaling and root planing (srp) alone or in combination with systemic metronidazole and/or professional cleaning in subjects with chronic periodontitis.200515811059
effect of khat chewing on 14 selected periodontal bacteria in sub- and supragingival plaque of a young male population.the habit of chewing khat (catha edulis) for its amphetamine-like effects is highly prevalent in yemen and east africa, and has expanded to western countries. the purpose of this study was to estimate and compare the prevalence and levels of 14 periodontal bacteria in gingival plaque of khat chewers and khat nonchewers, as well as of khat chewing sides and khat nonchewing sides.200515836514
molecular identification of the first human isolate belonging to the veillonella ratti-veillonella criceti group based on 16s rdna and dnak gene sequencing.anaerobic gram-negative cocci belonging to the genus veillonella are currently subdivided into eight species. among them, four have been isolated either from human flora or from clinical samples during infectious processes: veillonella atypica, v. dispar, v. parvula and v. montpellierensis. to date, the four other species have only been found in animals. in particular, v. ratti and v. criceti, since their characterization, have been exclusively reported from mouth and intestine of rodents. we re ...200515862461
microarrays complement culture methods for identification of bacteria in endodontic infections.the aim of this study was to investigate the microbial composition of necrotic root canals using culture methods and microarray technology. twenty uniradicular teeth with radiographic evidence of periapical bone loss and with no previous endodontic treatment were selected for this study. for molecular diagnosis a dna chip with 20 different species-specific, 16s-rdna-directed catcher probes was used. the microorganisms most frequently detected by the dna chip were: micromonas micros, fusobacteriu ...200515943771
does mechanical pressure force bacteria into the dentinal tubules of carious and non-carious teeth--an exploratory study.although many researchers have confirmed bacterial migration within dentine, no evidence was found to show whether bacteria can be easily forced into dentine during caries treatment.200515974430
periodontal disease and coronary heart disease: a reappraisal of the exposure.results from studies relating periodontal disease to cardiovascular disease have been mixed. residual confounding by smoking and use of clinical measures of periodontal disease rather than measures of infection have been 2 major criticisms. the aims of this study were to investigate relationships between prevalent coronary heart disease (chd) and 2 exposures, (1) clinical periodontal disease and (2) igg antibodies to 17 oral organisms, and to evaluate the role of smoking in these relationships.200515983248
[recovery of veillonella from saliva].veillonella spp. are anaerobic gram-negative cocci associated to oral health. different types of cultures have been reported for the isolation of these microorganisms. veillonella spp. colonies produce a red fluorescence, which is made visible through ultraviolet light and disappears in contact with oxygen. this feature would be very useful for rapid presumptive identification. the aims of this study were: 1. to compare the rogosa selective medium for veillonella with the cultures recommended by ...200515991476
multiplex fish analysis of a six-species bacterial biofilm.established procedures use different and seemingly incompatible experimental protocols for fluorescent in situ hybridization (fish) with gram-negative and gram-positive bacteria. the aim of this study was to develop a procedure, based on fish and confocal laser scanning microscopy (clsm), for the analysis of the spatial organization of in vitro biofilms containing both gram-negative and gram-positive oral bacteria. biofilms composed of the six oral species actinomyces naeslundii, candida albican ...200414706749
molecular dissection of the selenomonas ruminantium cell envelope and lysine decarboxylase involved in the biosynthesis of a polyamine covalently linked to the cell wall peptidoglycan layer.the wild type of selenomonas ruminantium subsp. lactilytica, which is a strictly anaerobic, gram-negative bacterium isolated from sheep rumen, requires one of the normal saturated volatile fatty acids with 3 to 10 carbon atoms for its growth in a glucose medium; however, no such obligate requirement of fatty acid is observed when the cells are grown in a lactate medium. this bacterium is characterized by a unique structure of the cell envelope and a novel lysine decarboxylase and its regulatory ...200414745158
[bacterial contamination of bony particles from the bone collection trap].intraoral bony defects can be filled with bony particles that are collected in a titanium filter while drilling. the rinsing liquid is contaminated with blood and saliva which implies that the bony particles are also contaminated with bacteria. the aim of this study was to determine quantitatively and qualitatively the degree of this contamination. over a period of three months bony particles were collected from 50 patients undergoing surgery. the bony particles were scraped off the filter, resu ...200415185482
[analysis of oral microflora early colonized in infants].to observe the dynamic changes of oral microflora early colonized in infants.200415190795
use of a continuous-flow anaerobic culture to characterize enteric virulence gene expression.we developed an in vitro culture method to characterize the expression of bacterial genes under conditions mimicking the colonic environment. our culture system (the intestinal simulator) comprised a continuous-flow anaerobic culture which was inoculated with fecal samples from healthy volunteers. as a test organism, we employed enteroaggregative escherichia coli (eaec), an emerging diarrheal pathogen that is thought to cause infection in both the small and large intestines. after the simulator ...200415213120
development of a multispecies oral bacterial community in a saliva-conditioned flow cell.microbial communities within the human oral cavity are dynamic associations of more than 500 bacterial species that form biofilms on the soft and hard tissues of the mouth. understanding the development and spatial organization of oral biofilms has been facilitated by the use of in vitro models. we used a saliva-conditioned flow cell, with saliva as the sole nutritional source, as a model to examine the development of multispecies biofilm communities from an inoculum containing the coaggregation ...200415240317
isolation of bacteriophages from the oral cavity.to isolate bacteriophages lytic for oral pathogens from human saliva, dental plaque and mature biofilms constituted from saliva-derived bacteria.200415242464
effect of amoxicillin use on oral microbiota in young children.dental plaque samples from 40 children were screened for the presence of bacteria resistant to amoxicillin. fifteen children had used amoxicillin and 25 had not used any antibiotic in the 3 months prior to sample collection. all (100%) of the children harbored amoxicillin-resistant oral bacteria. the median percentage of the total cultivable oral microbiota resistant to amoxicillin was 2.4% (range, 0.1 to 14.3%) in children without amoxicillin use and 10.9% (range, 0.8 to 97.3%) in children with ...200415273096
nime gene in a metronidazole-susceptible veillonella sp. strain. 200415273153
veillonella montpellierensis sp. nov., a novel, anaerobic, gram-negative coccus isolated from human clinical samples.three strains of a hitherto unknown, gram-negative, anaerobic coccus were isolated from human samples. at the phenotypic level, the isolates displayed all the characteristics of bacteria belonging to the genus veillonella. sequence analysis revealed that the three strains shared >99.5% similarity in 16s rdna sequence and >98.4% similarity in dnak sequence. the three unknown strains formed a separate subclade that was clearly remote from veillonella species of human and animal origin. based on th ...200415280307
was isolation of veillonella from spinal osteomyelitis possible due to poor tissue perfusion?from a needle biopsy of the body of the 12th thoracic vertebra in a 74-year-old man with spinal osteomyelitis a veillonella parvula was isolated. the significance of this bacterium as lactic acid indicator has been considered and discussed. since this strictly anaerobic bacterium mainly uses lactic acid for energy generation, lactic acid must continuously and sufficiently have been produced in or near to the vertebrate body to permit outgrowth of this bacterium. since neither microbial infection ...200415325011
t-rflp combined with principal component analysis and 16s rrna gene sequencing: an effective strategy for comparison of fecal microbiota in infants of different ages.the fecal microbiota of two healthy swedish infants was monitored over time by terminal restriction fragment length polymorphism (t-rflp) analysis of amplified 16s rrna genes. principal component analysis (pca) of the t-rflp profiles revealed that the fecal flora in both infants was quite stable during breast-feeding and a major change occurred after weaning. the two infants had different sets of microbiota at all sampling time points. 16s rdna clone libraries were constructed and the predominan ...200415325753
biofilm growth of lactobacillus species is promoted by actinomyces species and streptococcus mutans.the ability of oral bacteria to integrate within a biofilm is pivotal to their survival. a dependence on the amount of biofilm growth by noncoaggregating lactobacillus rhamnosus and lactobacillus plantarum on coculture with actinomyces naeslundii, actinomyces gerencseriae, streptococcus mutans and veillonella parvula was investigated using an artificial-mouth culture system. biofilm formation by the lactobacilli in mono-culture was poor. in coculture with actinomyces species the amount of l. rha ...200415327645
the prevalence of bacteraemia on removal of fixed orthodontic appliances.this study investigated the prevalence of bacteraemia on removal of fixed appliances. venous blood samples were taken before and after debonding and debanding for 30 patients (mean age 17 years 8 months) who had worn fixed appliances for an average of 19 months. before removal of the fixed appliances, bacteraemia was detected in one of the 30 subjects (3%) and in four subjects (13%) following removal of their fixed appliances. the 95 per cent confidence intervals for the prevalence of post-deban ...200415366390
interspecies communication in streptococcus gordonii-veillonella atypica biofilms: signaling in flow conditions requires juxtaposition.during the development of human oral biofilm communities, the spatial arrangement of the bacteria is thought to be driven by metabolic interactions between them. streptococcus gordonii and veillonella atypica, two early colonizing members of the dental plaque biofilm, have been postulated to participate in metabolic communication; s. gordonii ferments carbohydrates to form lactic acid, which is a preferred fermentation substrate for v. atypica. we found that, during agar-plate coculture of these ...200415546975
investigation of the effect of the coronal restoration quality on the composition of the root canal microflora in teeth with apical periodontitis by means of t-rflp analysis.to investigate the effect of the radiographic and clinical quality of coronal restorations on the composition of the root canal flora of teeth with necrotic pulps and teeth with root fillings associated with apical periodontitis.200415548272
do different implant surfaces exposed in the oral cavity of humans show different biofilm compositions and activities?osseointegrated dental implants play an important role in restorative dentistry. however, plaque accumulation may cause inflammatory reactions around the implants, sometimes leading to implant failure. in this in vivo study the influence of two physical hard coatings on bacterial adhesion was examined in comparison with a pure titanium surface. thin glass sheets coated with titanium nitride (tin), zirconium nitride (zrn) or pure titanium were mounted on removable intraoral splints in two adults. ...200415560835
isolation and characterisation of an equol-producing mixed microbial culture from a human faecal sample and its activity under gastrointestinal conditions.only about one third of humans possess a microbiota capable of transforming the dietary isoflavone daidzein into equol. little is known about the dietary and physiological factors determining this ecological feature. in this study, the in vitro metabolism of daidzein by faecal samples from four human individuals was investigated. one culture produced the metabolites dihydrodaidzein and o-desmethylangolensin, another produced dihydrodaidzein and equol. from the latter, a stable and transferable m ...200415578160
pattern of cytokine responses to gram-positive and gram-negative commensal bacteria is profoundly changed when monocytes differentiate into dendritic cells.the normal gastrointestinal bacterial flora is crucial for the maturation of acquired immunity via effects on antigen-presenting cells (apcs). here we investigated how two types of apcs, monocytes and dendritic cells (dcs), react to different bacterial strains typical of the commensal intestinal microflora. purified human monocytes and monocyte-derived dcs were stimulated with uv-inactivated gram-positive (lactobacillus plantarum and bifidobacterium adolescentis) and gram-negative (escherichia c ...200415102775
commensal gram-negative bacteria prime human dendritic cells for enhanced il-23 and il-27 expression and enhanced th1 development.dendritic cells (dc) are the main orchestrators of specific immune responses. depending on microbial information they encounter in peripheral tissues, they promote the development of th1, th2 or unpolarized th cell responses. in this study we have investigated the immunomodulatory effect of non-pathogenic intestinal gram-negative (escherichia coli, bacteroides vulgatus,veillonella parvula, pseudomonas aeruginosa) and gram-positive (bifidobacterium adolescentis, enterococcus faecalis, lactobacill ...200415114670
selection for high-level resistance by chronic triclosan exposure is not universal.to investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria.200415117935
acute disseminated encephalomyelitis following pleural empyema owing to boerhaave's syndrome.acute disseminated encephalomyelitis is reported in a 14-year-old male patient associated with a pleural empyema caused by an esophageal perforation. during the hospital course, the patient experienced an episode of cardiac arrest, raising the question of hypoxic-ischemic cerebral injury, but the magnetic resonance imaging (mri) findings and clinical course were most consistent with acute disseminated encephalomyelitis. after a course of methylprednisolone, broad-spectrum antibiotics, and bilate ...200415119485
systematic 16s rrna gene sequencing of atypical clinical isolates identified 27 new bacterial species associated with humans.clinical microorganisms isolated during a 5-year study in our hospital that could not be identified by conventional criteria were studied by 16s rrna gene sequence analysis. each isolate yielded a > or =1,400-bp sequence containing <5 ambiguities which was compared with the genbank 16s rrna gene library; 1,404 such isolates were tested, and 120 were considered unique (27 isolates) or rare (< or =10 cases reported in the literature) human pathogens. eleven new species, "actinobaculum massiliae," ...200415131188
antibiotic resistance profile of the subgingival microbiota following systemic or local tetracycline therapy.tetracyclines have been extensively used as adjunctives to conventional periodontal therapy. emergence of resistant strains, however, has been reported. this study evaluated longitudinally the tetracycline resistance patterns of the subgingival microbiota of periodontitis subjects treated with systemic or local tetracycline therapy+scaling and root planing (srp).200415142209
total hip prosthetic joint infection due to veillonella species. 200415050206
in vitro activity of ramoplanin and comparator drugs against anaerobic intestinal bacteria from the perspective of potential utility in pathology involving bowel flora.susceptibility of intestinal bacteria to various antimicrobial agents in vitro, together with levels of those agents achieved in the gut, provides information on the likely impact of the agents on the intestinal flora. orally administered drugs that are poorly absorbed may be useful for treatment of intestinal infections and for certain other situations in which intestinal bacteria may play a role. the antimicrobial activity of ramoplanin (mdl 62,198) against 928 strains of intestinal anaerobic ...200416701520
prevalence, proportions, and identities of antibiotic-resistant bacteria in the oral microflora of healthy children.the aims of this study were to determine the prevalence, proportions and identities of oral bacteria resistant to six antibiotics in 35 children (4-5 years old) who had not received antibiotics during the previous 3 months. ampicillin-, penicillin-, erythromycin-, and tetracycline-resistant bacteria were harbored by 35 (100%), 34 (97%), 35 (100%), and 34 (97%) children, respectively. none of the children harbored metronidazole-resistant anaerobic bacteria or gram-positive vancomycin-resistant ba ...200315000743
[megaesophagus microbiota and carcinogenesis].the risk of development of spin cell carcinoma of the esophagus is 33 times higher in patients with chagasic achalasia. it is possible that the production of n-nitroso compounds in the esophageal lumen by of bacterial action in the stasis liquid that reduce nitrates from diet into nitrites may play a role in this process.200314534659
in vitro activities of a new des-fluoro(6) quinolone, garenoxacin, against clinical anaerobic bacteria.the antimicrobial activities of garenoxacin and eight other antibiotics against 641 anaerobic isolates were evaluated with the nccls agar dilution method. overall, the mics of garenoxacin for 50 and 90% of the strains tested (in micrograms per milliliter) were as follows: bacteroides fragilis group, 0.5 and 2; prevotella spp., 0.25 and 2; fusobacterium spp., 0.25 and 0.5; porphyromonas spp., 0.125 and 0.25; bilophila wadsworthia, 0.5 and 1; veillonella spp., 0.25 and 0.5; clostridium spp., 0.25 ...200314576144
bacteriologic findings in patients with chronic sinusitis.we studied the bacteriology of maxillary sinus aspirates obtained from patients diagnosed with chronic sinusitis. we recovered 659 strains from 510 aspirates; of these, 572 (86.8%) were aerobes and 87 (13.2%) were anaerobes. aerobes only were recovered from 310 of the 510 specimens (60.8%) and anaerobes only from 31 (6.1%). among the 572 aerobic bacteria, the most prevalent organisms were streptococcus viridans (158 strains [27.6%]), streptococcus pneumoniae (67 [11.7%]), corynebacterium species ...200314606178
prevalence and identification of tetracycline-resistant oral bacteria in children not receiving antibiotic therapy.the prevalence of tetracycline-resistant oral bacteria in healthy 4- and 6-year-old children who had not received antibiotics during the 3 months prior to sampling was investigated. of the 47 children sampled, 46 harboured tetracycline-resistant bacteria. the median proportion of cultivable anaerobic and aerobic oral bacteria resistant to tetracycline was 1.1% and the mic50 of these was 64 microg ml(-1). the majority (56%) of tetracycline-resistant bacteria were resistant to at least one other a ...200314612243
susceptibility of oral bacteria to melaleuca alternifolia (tea tree) oil in vitro.the in vitro activity of melaleuca alternifolia (tea tree) oil against 161 isolates of oral bacteria from 15 genera was determined. minimum inhibitory concentrations (mic) and minimum bactericidal concentrations (mbc) ranged from 0.003 to 2.0% (v/v). mic90 values were 1.0% (v/v) for actinomyces spp., lactobacillus spp., streptococcus mitis and streptococcus sanguis, and 0.1% (v/v) for prevotella spp. isolates of porphyromonas, prevotella and veillonella had the lowest mics and mbcs, and isolates ...200314622345
an in vitro study into the corrosion of intra-oral magnets in the presence of dental amalgam.the aim of this investigation was to study the corrosion behaviour and products of uncoated neodymium-iron-boron magnets in the presence of dental amalgam. microcosm plaques were grown on discs of neodymium-iron-boron magnets or amalgam in a constant depth film fermentor. the biofilms were supplied with artificial saliva and growth was determined by viable counting. the results showed that the neodymium-iron-boron magnets corroded with an average daily weight loss of 0.115 +/- 0.032 per cent. ho ...200314700268
antibiotic susceptibility of bacterial isolates in gingivitis and periodontitis.the present study was conducted to isolate aerobic as well as anaerobic microbes from patients of gingivitis and adult periodontitis and to study the susceptibility patterns of these isolates to different antibiotics. in our study all the samples belonging to the control as well as the study groups yielded microbes. aerobes and facultative anaerobes were isolated from 100 percent and 96 percent cases of normal gingiva and orodental infections respectively. anaerobes were isolated from 80 percent ...200314705453
the microflora recovered from the outer-surfaces of the frialit-2 implanto-prosthetic connector.the aim of the present investigation was to examine the outer-surface microbiota of the prosthetic connector of frialit-2 implants, and to compare the microbial findings with the peri-implant parameters 2 years after functional loading. in 16 implant-treated patients (55.8 +/- 9.5 years) the outer-surface micro-organisms of the prosthetic connectors were determined in 32 frialit-2 implants. the functional loading time of the prosthetic suprastructures was 24.1 +/- 13.8 months on average. after r ...200312562362
mass transport of macromolecules within an in vitro model of supragingival plaque.the aim of this study was to examine the diffusion of macromolecules through an in vitro biofilm model of supragingival plaque. polyspecies biofilms containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, streptococcus sobrinus, veillonella dispar, and candida albicans were formed on sintered hydroxyapatite disks and then incubated at room temperature for defined periods with fluorescent markers with molecular weights ranging from 3,000 to 900,000. subsequent examinatio ...200312620862
in vitro activities of telithromycin and 10 oral agents against aerobic and anaerobic pathogens isolated from antral puncture specimens from patients with sinusitis.a study of the comparative in vitro activity of telithromycin, a new ketolide, against 155 aerobic and 171 anaerobic antral sinus puncture isolates showed it to be active against a broad range of sinus pathogens. all pneumococci, including erythromycin-resistant strains, were susceptible to telithromycin at < or = 0.5 microg/ml; all haemophilus influenzae and eikenella corrodens strains were inhibited by < or = 4 microg of telithromycin/ml; all moraxella spp. and beta-lactamase-producing prevote ...200312760875
intra-chromosomal heterogeneity between the four 16s rrna gene copies in the genus veillonella: implications for phylogeny and taxonomy.among the seven species characterized within the genus veillonella, three (veillonella dispar, veillonella parvula and veillonella atypica) have so far been isolated from human flora and during infectious processes. sequencing and analysis of 16s rdna (rrs) has been described as the best method for identification of veillonella strains at the species level since phenotypic characteristics are unable to differentiate between species. rrs sequencing for the three species isolated from humans showe ...200312777489
the effects of a triclosan/copolymer dentifrice on oral bacteria including those producing hydrogen sulfide.a clinical procedure was developed to examine the effects of short-term and extended use of a triclosan/copolymer dentifrice and a commercial fluoride dentifrice on oral bacteria, including those producing hydrogen sulfide. healthy adults volunteered for this double-blind, crossover design clinical study and provided saliva samples for culturing on enriched and indicator media to enumerate all salivary bacteria and those producing hydrogen sulfide (odorigenic), respectively. subjects brushed wit ...200312786953
microbiology of mandibular third molar pericoronitis: incidence of beta-lactamase-producing bacteria.the purpose of this study was to evaluate the predominant flora associated with pericoronitis in third molars and to investigate the presence of beta-lactamase-producing strains.200312789143
subgingival microbiota levels and their associations with periodontal status at the sampled sites in an adult sudanese population using miswak or toothbrush regularly.little information is available on the effect of miswak use on gingival microbiota. we assessed levels of 28 oral bacteria in subgingival plaque of adult sudanese miswak (n = 38) and toothbrush users (n = 36) age range 20-53 years (mean 34.6 years) to study associations between these bacteria, oral hygiene method, and periodontal status at the sampled sites. a pooled subgingival plaque sample from 6 probing sites of 1 selected tooth in each jaw was obtained from each subject. whole genomic dna p ...200312790510
penicillin-binding proteins involved in high-level piperacillin resistance in veillonella spp.to investigate high-level piperacillin resistance in veillonella spp. in the absence of beta-lactamase activity.200312805259
in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, bacitracin, and four other antimicrobials against intestinal anaerobic bacteria.by using an agar dilution method, the in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, and five other agents were determined against 300 gram-positive and 54 gram-negative strains of intestinal anaerobes. ramoplanin was active at <or=2 microg/ml against 287 of 300 (95.7%) gram-positive organisms, including 18 strains of clostridium difficile for which mics of ramoplanin were 0.25 to 0.5 microg/ml; for 3 of these, linezolid mics were 8 to 16 micro g/ml. nineteen clostridium ...200312821492
distribution of selected bacterial species on intraoral surfaces.to examine the proportions of 40 bacterial species in samples from 8 oral soft tissue surfaces and saliva in systemically healthy adult subjects and to compare these microbiotas with those of supra- and subgingival plaque.200312834503
effects of a chlorhexidine gluconate-containing mouthwash on the vitality and antimicrobial susceptibility of in vitro oral bacterial ecosystems.oral bacterial microcosms, established using saliva inocula from three individuals, were maintained under a feast-famine regime within constant-depth film fermenters. steady-state communities were exposed four times daily, postfeeding, to a chlorhexidine (chx) gluconate-containing mouthwash (chxm) diluted to 0.06% (wt/vol) antimicrobial content. the microcosms were characterized by heterotrophic plate counts and pcr-denaturing gradient gel electrophoresis (dgge). chxm caused significant decrease ...200312902270
age-related frequency of penicillin resistance of oral veillonella.veillonella spp. are early colonizing inhabitants in the mouth. as part of studies on penicillin resistance among oral indigenous anaerobic microbiota in childhood, the aim of the present longitudinal study was to examine the emergence of resistant strains in veillonella populations. altogether 305 veillonella isolates from saliva of 49 healthy infants followed from 2 to 24 months of age were examined for their in vitro susceptibility to penicillin g and, further, 20 penicillin-resistant isolate ...200312944020
effect of subtherapeutic concentrations of tylosin on the inhibitory stringency of a mixed anaerobe continuous-flow culture of chicken microflora against escherichia coli o157:h7.the aim of this study was twofold: first to determine the effect of subtherapeutic concentrations of tylosin, a macrolide antibiotic used for growth promotion, on a mixed anaerobic continuous-flow fermentation culture of chicken gastrointestinal microorganisms (ccf) and secondly, to determine if these concentrations would allow persistence of escherichia coli o157:h7 in ccf.200312492926
anaerobic bacteria cultured from the tongue dorsum of subjects with oral malodor.the bacteria on the dorsum of the tongue are the most frequent cause of oral malodor; however, the bacterial flora of the tongue has not been well defined. although recent studies have used dna probes to detect the presence of certain periodontal pathogens, cultural studies have been limited because of the complexity of the flora of the tongue dorsum. the purpose of this study was to grow and to identify maximum numbers of capnophylic gram-negative bacilli and anaerobic micro-organisms by cultur ...200316887710
removal and prevention of dental plaque with d-tagatose.dental plaque develops when early bacterial colonizers adhere to the acquired pellicle (saliva-derived proteinous coating on the tooth surface) followed by adhesion of late interspecies colonizers to form this type of biofilm (coaggregation). in developing a d-tagatose-based toothpaste, we examined 15 oral isolates, including both early colonizers (streptococcus and actinomyces) and late colonizers (fusobacterium, porphyromonas, prevotella, veillonella, capnocytophaga, and actinobacillus), and t ...200218498514
bronchopneumonia and oral health in hospitalized older patients. a pilot study.to correlate microbial findings obtained by bronchoalveolar lavage in pneumonia patients with the clinical situation of the oral cavity.200212542215
in vitro activity of gemifloxacin compared to seven other oral antimicrobial agents against aerobic and anaerobic pathogens isolated from antral sinus puncture specimens from patients with sinusitis.using a microbroth method for aerobes and agar dilution for anaerobes, we studied the comparative in vitro activity of gemifloxacin, three fluoroquinolones, two macrolides and two beta-lactams, against 207 aerobic and 162 anaerobic antral sinus puncture isolates. gemifloxacin was active at < or = 0.25 ug/ml against 198/207 [96%] aerobes and 127/162 [78%] anaerobes or 325/369 [88%] of all isolates and was the most active quinolone on a weight basis against gram-positive organisms. all haemophilus ...200211858906
bal 9141, a new broad-spectrum pyrrolidinone cephalosporin: activity against clinically significant anaerobes in comparison with 10 other antimicrobials.the in vitro potency of bal 9141, a new pyrrolidinone cephalosporin, was tested against non-duplicate strains of anaerobic bacteria. the mic(50) was 1 mg/l against actinomyces species, clostridium species, gram-positive anaerobic cocci, porphyromonas species, fusobacterium species, lactobacillus species, prevotella species and veillonella species. the mic(50) was 16 mg/l for bacteroides fragilis and other bacteroides species. bal 9141 was not active against cefoxitin-resistant bacteroides fragil ...200211864955
stress and the periodontal diseases: effects of catecholamines on the growth of periodontal bacteria in vitro.microorganisms possess the ability to recognize hormones within the host and utilize them to adapt to their surroundings. noradrenaline and adrenaline, which are released during human stress responses, may act as environmental cues to alter the growth of individual organisms within subgingival biofilms. the aims of this study were to modify, for anaerobic culture, existing methodology used in determining microorganism catecholamine responses and to investigate the growth responses to noradrenali ...200212354211
megaesophagus microbiota: a qualitative and quantitative analysis.bacterial overgrowth in the esophageal lumen in patients with megaesophagus can be the cause of recurring pulmonary infections, infectious complications due to surgical or endoscopic procedures, and the development of dysplasia of the esophageal mucosa and cancer. despite this, esophageal microbiota in the megaesophagus have never been studied. the aim of this study was to analyze qualitatively and quantitatively the microbiota in chagasic megaesophagus in comparison to the normal esophagus. twe ...200212399062
innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora.the hygiene hypothesis postulates that the prevalence of allergy has increased due to decreased microbial stimulation early in life, leading to delayed maturation of the immune system. the aim of this study was to examine the cytokine pattern produced from cord blood mononuclear cells relative to adult cells after stimulation with bacterial strains from the normal flora. mononuclear cells from cord and adult blood samples were stimulated with the following bacteria: bifidobacterium adolescentis, ...200212438343
correlations between bacterial levels in autologous subgingival plaque and saliva of adult sudanese.the aim of this study was to assess levels of oral bacteria and their correlations in paired samples of saliva and subgingival plaque in a population of adult sudanese. whole saliva and pooled subgingival plaque samples from six probing sites of one tooth in each jaw were obtained from 56 sudanese adults (mean age 35.2+/-8.9 years). levels of 24 oral bacteria in the autologous saliva and pooled plaque sample of each subject were assessed by dna probes and checkerboard dna-dna hybridization. ther ...200212483235
molecular analysis of bacterial species associated with childhood caries.although substantial epidemiologic evidence links streptococcus mutans to caries, the pathobiology of caries may involve more complex communities of bacterial species. molecular methods for bacterial identification and enumeration now make it possible to more precisely study the microbiota associated with dental caries. the purpose of this study was to compare the bacteria found in early childhood caries (ecc) to those found in caries-free children by using molecular identification methods. clon ...200211880430
meningitis and shunt infection caused by anaerobic bacteria in children.this review describes the microbiology and management of meningitis and shunt infections caused by anaerobic bacteria in children. the predominant anaerobes recovered in meningitis are bacteriodes spp., bacteriodes fragilis, fusobacterium spp., and clostridium spp. peptostreptococcus, veillonella, actinomyces, propionibacterium acnes, and eubacterium are less commonly isolated. the predisposing conditions for meningitis are acute or chronic middle-ear infection, sinusitis, pharyngitis, and pulmo ...200211897473
particulate bioglass reduces the viability of bacterial biofilms formed on its surface in an in vitro model.45s5 bioglass is a bioactive implant material which, in its particulate form, is used in the repair of periodontal defects. the surface reactions undergone by this material in an aqueous environment may exert an antibacterial effect that would be beneficial to periodontal surgical treatment. biofilms of streptococcus sanguis, an early plaque former, and mixed species biofilms from a salivary inoculum grown under conditions similar to those associated with periodontal implants, were grown on part ...200212005145
effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms.antimicrobial effects of fluoride in vivo remain contentious. previous studies suggested that 1 mm naf reduced acid production from glucose, and prevented the enrichment of bacteria associated with caries in a chemostat model. the present study examines the effects of a lower fluoride concentration (0.53 mm, 10 ppm naf) in both biofilm and planktonic microbial communities. nine oral species were grown at ph 7.0 and pulsed on 10 successive days with glucose; bacterial metabolism was allowed to re ...200212037363
extensive set of 16s rrna-based probes for detection of bacteria in human feces.for the detection of six groups of anaerobic bacteria in human feces, we designed seven new 16s rrna-based oligonucleotide probes. this set of probes extends the current set of probes and gives more data on the composition of the human gut flora. probes were designed for phascolarctobacterium and relatives (phasco741), veillonella (veil223), eubacterium hallii and relatives (ehal1469), lachnospira and relatives (lach571), and eubacterium cylindroides and relatives (ecyl387), and two probes were ...200212039758
energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-coa or acrylyl-coa.fermentation balances and growth yields were determined with various bacteria fermenting lactate to acetate plus propionate either via methylmalonyl-coa or via acrylyl-coa. all strains fermented lactate to acetate plus propionate at approximately a 1:2 ratio. growth yields of propionibacterium freudenreichii were more than twice as high as those of clostridium homopropionicum or veillonella parvula. hydrogen was formed as a side product to a significant extent only by v. parvula and pelobacter p ...200212052552
invasion of dentinal tubules by oral bacteria.bacterial invasion of dentinal tubules commonly occurs when dentin is exposed following a breach in the integrity of the overlying enamel or cementum. bacterial products diffuse through the dentinal tubule toward the pulp and evoke inflammatory changes in the pulpo-dentin complex. these may eliminate the bacterial insult and block the route of infection. unchecked, invasion results in pulpitis and pulp necrosis, infection of the root canal system, and periapical disease. while several hundred ba ...200212097359
changes in the intestinal flora after the administration of prophylactic antibiotics to patients undergoing a gastrectomy.changes in the intestinal flora were investigated in patients administered antibiotics for 4 days after a gastrectomy.200212111513
selection of dairy bacterial strains as probiotics for oral health.the aim of the present study was to select bacterial strains with potential properties as oral probiotics, namely for the prevention of dental caries. we examined 23 dairy microorganisms, out of which we identified two streptococcus thermophilus and two lactcoccus lactis strains that were able to adhere to saliva-coated hydroxyapatite beads to the same extent as streptococcus sobrinus omz176. two of them, strep. thermophilus ncc1561 and lactoc. lactis ssp. lactis ncc2211, were further successful ...200212120707
orbital cellulitis and abscess secondary to dacryocystitis. 200212149070
combinations of bacterial species in endodontic infections.this study was undertaken to investigate combinations of bacteria found in root-canal infections of teeth with periapical bone destruction without clinical signs and symptoms.200212196223
comparative in vitro activity of faropenem and 11 other antimicrobial agents against 405 aerobic and anaerobic pathogens isolated from skin and soft tissue infections from animal and human bites.faropenem, a new oral beta-lactam agent with a penem structure, was very active against 405 aerobic and anaerobic bite isolates. it inhibited 232 of 236 (98%) aerobic isolates, including all pasteurella spp. and eikenella corrodens at < or = 0.25 mg/l, and 164/169 (97%) anaerobic isolates, at < or = 1 mg/l. the 10 isolates that required > or = 2 mg/l for inhibition were one strain each of acinetobacter lwoffi, corynebacterium minutissimum, bacteroides ovatus, lactobacillus delbrueckii and peptos ...200212205068
bacterial diversity in necrotizing ulcerative periodontitis in hiv-positive subjects.necrotizing ulcerative periodontitis (nup) is a painful and potentially debilitating affliction that affects about 2% to 6% of hiv-positive subjects. nup may be caused by specific microorganisms that are presently unknown or by microbial species not usually thought to cause periodontal infections. the purpose of this study was to define the bacterial species associated with nup in hiv-positive patients.200216013212
[the microflora of ulzerous zone mucosa in patients with duodenal ulcer].bacteriological study of the biopsies taken from gastric and duodenal mucosa of 10 healthy volunteers and 74 patients with duodenal ulcer, was carried out. in the gastroduodenal zone of healthy subjects microorganisms of 6 genera (streptococcus, candida, staphylococcus, bacillus, helicobacter and lactobacillus) were detected. h. pylori was isolated in 20% of cases only in biopsy specimens taken from the antral section of the stomach of healthy as monoculture or in combination with c. albicans. i ...200111871291
spatial arrangements and associative behavior of species in an in vitro oral biofilm model.the spatial arrangements and associative behavior of actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis strains in an in vitro model of supragingival plaque were determined. using species-specific fluorescence-labeled antibodies in conjunction with confocal laser scanning microscopy, the volumes and distribution of the five strains were assessed during biofilm formation. the volume-derived cell numbers of each strain correlated w ...200111229930
sodium ion-translocating decarboxylases.the review is concerned with three na(+)-dependent biotin-containing decarboxylases, which catalyse the substitution of co(2) by h(+) with retention of configuration (deltag degrees '=-30 kj/mol): oxaloacetate decarboxylase from enterobacteria, methylmalonyl-coa decarboxylase from veillonella parvula and propiogenium modestum, and glutaconyl-coa decarboxylase from acidaminococcus fermentans. the enzymes represent complexes of four functional domains or subunits, a carboxytransferase, a mobile al ...200111248185
validation of an in vitro biofilm model of supragingival plaque.the study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. we describe a model of supragingival plaque containing actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis wherein cells are cultivated anaerobically in a saliva-based medium on hydroxyapatite discs coated with a salivary pellicle, with material and pieces of apparatus common to al ...200111269730
prosthetic joint infection due to veillonella dispar.described here is the first report of a monomicrobial joint infection caused by veillonella dispar that resulted in loosening of a prosthesis. this clinical case shows that veillonella spp. should not be disregarded as contaminant organisms, particularly when they are isolated in pure culture from clinical specimens involving cases of septic arthritis.200111453595
antibacterial properties of electron beam-sterilized gutta-percha cones.the purpose of the study was to determine the effect of electron beam sterilization on gutta-percha cones (gpcs) at different times after sterilization. an agar diffusion test was used with -one aerobic bacterium (bacillus subtilis) and five oral anaerobic bacteria (fusobacterium nucleatum, peptostreptococcus micros, porphyromonas gingivalis, propionibacterium acnes, and veillonella parvula). with each microorganism 30 agar plates were prepared, evenly distributed among three groups (group 1: un ...200111487145
molecular identification of microorganisms from endodontic infections.a relatively wide range of bacteria have been isolated from root canals using standard culture techniques. however, only 50% of the bacteria in the oral cavity are cultivable (s. s. socransky et al., arch. oral biol. 8:278-280, 1963); hence, bacterial diversity in endodontic infections is underestimated. this study used a pcr-based 16s rrna gene assay, followed by cloning and sequencing of 16s rrna amplicons from a small subset of samples to assess the diversity of bacteria present in infected r ...200111526164
efficacy of manual and powered toothbrushes (ii). effect on microbiological parameters.the purpose of the present investigation was to determine the effect of self-performed supragingival plaque removal using either manual (crest complete) or power (braun 3d plaque remover) toothbrushing on supra and subgingival plaque composition.200111686813
association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid.the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions.200111737506
supragingival and subgingival microbiota of adult patients with down's syndrome. changes after periodontal treatment.in this longitudinal study, five adult down's syndrome patients with periodontitis were placed on a frequent recall visit schedule (every 6 weeks) after treatment, in order to investigate: 1) the microbiological status, both supragingivally and subgingivally, and the changes that occurred after treatment and 2) the effect of frequent professional supragingival plaque control on the subgingival microbiota and clinical variables in these patients. the clinical variables recorded were probing pocke ...200111737662
the predominant microflora of nursing caries lesions.the predominant microflora recovered from infected dentine of 52 carious teeth from 14 children with nursing caries was determined using both selective and non-selective media for the isolation of specific genera and acidified media (ph 5.2) to isolate the predominant aciduric microorganisms, and compared with the microflora of sound enamel surfaces in caries-free children. streptococcus mutans formed a significantly greater proportion of the lesion flora while streptococcus oralis, streptococcu ...200111799279
deep neck infections in children.from december 1989 through 1998, a total of 68 children with deep neck infection were enrolled into this study. the mean age was 5.9 years (range, 1 month-15.8 years). infections in the retropharyngeal space (36.7%) were most common, followed by parapharyngeal space (30.8%), peritonsillar space (20.6%), and submandibular space (11.9%). fever, neck pain, and swelling were the most frequent symptoms. the most common pathogens were viridans streptococci (41%, 16/39) and staphylococcus aureus (26%, ...200111825010
the effect of scaling and root planing on the clinical and microbiological parameters of periodontal diseases: 12-month results.previously, we reported that srp resulted in a decrease in mean pocket depth and attachment level and reduced prevalence and levels of bacteroidesforsythus, porphyromonas gingivalis, and treponema denticola at 3 and 6 months post-srp in 57 subjects with adult periodontitis. 32 of the 57 subjects were monitored at 9 and 12 months. thus, the purpose of the present investigation was to evaluate the microbial and clinical effects of srp in 32 (mean age 48+/-11) subjects over a 12-month period.200010674959
[backache in a 70-yr-old man]. 200010974770
veillonella parvula meningitis: case report and review of veillonella infections.veillonella parvula is a small, nonfermentative anaerobic gram-negative coccus that is part of the normal flora of the mouth, gastrointestinal tract, and vagina in humans. when isolated from clinical specimens, v. parvula is often regarded as a contaminant or commensal, but it has been implicated as a pathogen in infections of the sinuses, lungs, heart, bone, and central nervous system. meningitis, however, is extremely rare; to our knowledge, only 2 cases have been previously described in the l ...200011017846
automated immunofluorescence for enumeration of selected taxa in supragingival dental plaque.the present study investigated a recently developed automated image analysis technique for its applicability to the enumeration of selected bacteria in supragingival dental plaque. following initial calibration, the system is capable to count fluorescence-labeled target cells in up to 48 samples without user interference. test samples contained a characteristic mixture of planktonic bacteria, small almost planar bacterial aggregates, and large, virtually indisruptable clumps with cells from mult ...200011037755
the formation of mixed culture biofilms of oral species along a gradient of shear stress.a chemostat mixed culture system was used to produce two distinct ecological states, state-1 (caries-like microcosm) and state-2 (periodontal-like microcosm). eleven bacterial species (streptococcus gordonii, strep. mitis i, strep. mutans, strep. oralis, actinomyces naeslundii, lactobacillus casei, neisseria subflava, fusobacterium nucleatum, porphyromonas gingivalis, prevotella nigrescens, veillonella dispar) were used to inoculate the planktonic system. a flow cell, designed to produce converg ...200011054158
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