Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| whole-genome sequence analysis of g3 and g14 equine group a rotaviruses isolated in the late 1990s and 2009-2010. | equine group a rotavirus (rva) g3p[12] and g14p[12] strains cause gastroenteritis in foals worldwide. both of these strains have been co-circulating in japan since g14p[12] strains emerged in the late 1990s. although it is important to comprehensively understand the evolution of rva strains, whole-genome sequence data on recent equine rva strains in japan are lacking. therefore, in this study, whole-genome analysis of 23 equine rva isolates from the late 1990s and 2009-2010 and the vaccine strai ... | 2015 | 25708838 |
| changing patterns of rotavirus strains circulating in ireland: re-emergence of g2p[4] and identification of novel genotypes in ireland. | worldwide, group a rotavirus (rva) is recognized as the most common aetiological agent of acute diarrheal disease in children. one hundred and ninety seven positive faecal samples were obtained from patients between 2006 and 2008. reverse transcriptase polymerase chain reaction (rt-pcr) was used to amplify the vp7 and vp4 gene segments of these samples, and g and p typing was carried out subsequently. the most common strain type was g1p[8], and the emergent global g9-type was identified in both ... | 2015 | 25704492 |
| [characterization of group a rotavirus genotypes isolated from gastroenteritis among children during the 2008/2009 through 2013/2014 seasons in nara prefecture, japan]. | 2015 | 26630796 | |
| detection and genetic characterization of porcine group a rotaviruses in asymptomatic pigs in smallholder farms in east africa: predominance of p[8] genotype resembling human strains. | viral enteritis is a serious problem accounting for deaths in neonatal animals and humans worldwide. the absence of surveillance programs and diagnostic laboratory facilities have resulted in a lack of data on rotavirus associated diarrheas in pigs in east africa. here we describe the incidence of group a rotavirus (rva) infections in asymptomatic young pigs in east africa. of the 446 samples examined, 26.2% (117/446) were positive for rva. more nursing piglets (78.7%) shed rva than weaned (32.9 ... | 2015 | 25541378 |
| prevalence of rotaviruses groups a and c in egyptian children and aquatic environment. | the objective of this study is to compare the prevalence of rotaviruses groups a and c in egyptian children and aquatic environment. from 110 stool specimens of children with acute diarrhea and using rt-pcr, 35 samples (31.8 %) were positive for human rotavirus group a and 15 samples (13.6 %) were positive for human rotavirus group c. from 96 samples collected from zenin wastewater treatment plant over a 2-year period (november 2009-october 2011) and using rt-pcr, rotavirus group a was detected ... | 2015 | 25665825 |
| molecular epidemiology of group a rotavirus among children admitted to hospital in salto, uruguay, 2011-2012: first detection of the emerging genotype g12. | group a rotavirus (rva) is the most important etiologic agent of infant acute gastroenteritis (age) worldwide. detection and molecular characterization of rva in salto department, northwestern region of uruguay, was conducted on 175 clinical samples, being 153 stool and 22 vomit samples, collected from hospitalized children with age, between 0-15 years old, from two hospitals of salto city during 2011 and 2012. rva was detected and genotyped by seminested multiplex rt-pcr in order to determine g ... | 2015 | 25650154 |
| molecular characterization of equine rotaviruses isolated in europe in 2013: implications for vaccination. | equine group a rotavirus (rvas) mainly cause disease in foals under the age of 3 months. only sporadic data are available on the circulation of rvas in equine populations in europe. in this study, 65 diarrheic samples from foals under 4 months of age were collected in belgium (n=32), germany (n=17), slovenia (n=5), sweden (n=4), hungary (n=3), italy (n=2), france (n=1) and the netherlands (n=1). forty percent of these samples (n=26) were found to be rva positive by a quantitative rt-pcr assay. t ... | 2015 | 25637313 |
| rotavirus and adenovirus gastroenteritis: time series analysis. | this study investigated the effects of changes in weather conditions (monthly average temperature, monthly minimum temperature, monthly average humidity) on rotavirus and adenovirus gastroenteritis frequency and whether there was a seasonal correlation. | 2015 | 25625610 |
| genetic diversity in three bovine-like human g8p[14] and g10p[14] rotaviruses suggests independent interspecies transmission events. | the group a rotavirus (rva) p[14] genotype has been detected sporadically in humans and is thought to be acquired through zoonotic transmission. the present study describes the full-length genome analysis of two g8p[14] and one g10p[14] human rvas detected in italy. the strains possessed the typical bovine-like i2-r2-c2-m2-a3/a11-n2-t6-e2-h3 genotype constellation. all the segments of the two g8p[14] rvas were most closely related to bovine(-like) strains but were relatively distant to each othe ... | 2015 | 25614586 |
| molecular characterization of group a rotavirus before and after the introduction of vaccines in brazil. | in this study, the molecular characteristics of group a rotavirus (rva) were compared in samples obtained before and after rva vaccine-introduction in brazil. | 2015 | 26516971 |
| comparison of enzyme-linked immunosorbent assay and immunochromatography for rotavirus detection in children below five years with acute gastroenteritis. | group-a rotaviruses are responsible for 30 to 60% of severe watery diarrhea cases in young children. timely diagnosis of rotavirus infection helps to determine appropriate treatment and prevents unnecessary use of antibiotics. | 2015 | 26500903 |
| rotavirus vp7 epitope chimeric proteins elicit cross-immunoreactivity in guinea pigs. | vp7 of group a rotavirus (rva) contains major neutralizing epitopes. using the antigenic protein vp6 as the vector, chimeric proteins carrying foreign epitopes have been shown to possess good immunoreactivity and immunogenicity. in the present study, using modified vp6 as the vector, three chimeric proteins carrying epitopes derived from vp7 of rva were constructed. the results showed that the chimeric proteins reacted with anti-vp6 and with sa11 and wa virus strains. antibodies from guinea pigs ... | 2015 | 26459269 |
| detection of rotavirus in children with acute gastroenteritis in zagazig university hospitals in egypt. | rotavirus is the major cause of acute gastroenteritis (age) in infants and young children all over the world. the objective of the study was to compare different methods for detecting rotavirus and to assess the burden of rotavirus as a causative agent for age in children younger than five. | 2015 | 26435821 |
| diversity of group a rotavirus on a uk pig farm. | group a rotaviruses (garv) are a significant cause of enteritis in young pigs. the aim of this study was to extend our understanding of the molecular epidemiology of porcine garv in the uk by investigating the genetic diversity of garv on a conventional farrow-to-finish farm. faecal samples were obtained from six batches of pigs in 2009 and 8 batches in 2010, when the pigs were 2, 3 (time point omitted in 2009), 4, 5, 6 and 8 weeks of age. presence of rotavirus was detected by reverse transcript ... | 2015 | 26432051 |
| zoonotic transmission of rotavirus: surveillance and control. | group a rotavirus (rotavirus a, rva) is the main cause of acute dehydrating diarrhea in humans and numerous animal species. rva shows vast diversity and a variety of human strains share genetic and antigenic features with animal origin rva strains. this finding suggests that interspecies transmission is an important mechanism of rotavirus evolution and contributes to the diversity of human rva strains. rva is responsible for half a million deaths and several million hospitalizations worldwide. g ... | 2015 | 26428261 |
| qualitative effects of monovalent vaccination against rotavirus: a comparison of north america and south america. | rotavirus is the most common cause of severe gastroenteritis in young children worldwide. the introduction of vaccination programs has led to a significant reduction in number of hospitalizations due to rotavirus in north and south american countries. little work has been done, however, to examine the differential impact of vaccination as a function of strain distribution and strain-specific vaccine efficacy. we developed a two-strain epidemiological model of rotavirus transmission, and used it ... | 2015 | 26416267 |
| rotavirus nsp6 localizes to mitochondria via a predicted n-terminal a-helix. | specific roles have been ascribed to each of the 12 known rotavirus proteins apart from the non-structural protein 6 (nsp6). however, nsp6 may be present at sites of viral replication within the cytoplasm. here we report that nsp6 from diverse species of rotavirus a localizes to mitochondria via conserved sequences in a predicted n-terminal a-helix. this suggests that nsp6 may affect mitochondrial functions during rotavirus infection. | 2015 | 26404393 |
| whole genome analysis of vietnamese g2p[4] rotavirus strains possessing the nsp2 gene sharing an ancestral sequence with chinese sheep and goat rotavirus strains. | because imminent introduction into vietnam of a vaccine against rotavirus a is anticipated, baseline information on the whole genome of representative strains is needed to understand changes in circulating strains that may occur after vaccine introduction. in this study, the whole genomes of two g2p[4] strains detected in nha trang, vietnam in 2008 were sequenced, this being the last period during which virtually no rotavirus vaccine was used in this country. the two strains were found to be >99 ... | 2015 | 26382233 |
| a novel multiplex one-step real-time rt-pcr assay for the simultaneous identification of enterovirus and parechovirus in clinical fecal samples. | enterovirus (ev) and parechovirus (pev) can either infect humans asymptomatically or can cause gastroenteritis, respiratory symptoms and, sometimes, severe disease. as the number of newly identified ev and pev genotypes keeps increasing, diagnostic methods need to be updated. to this end, we described a novel multiplex one-step real-time rt-pcr to detect ev and human pev (hpev) simultaneously in fecal samples collected from children with rotavirus group a (rv-a)-related gastroenteritis. | 2015 | 26789989 |
| clinical symptoms of human rotavirus infection observed in children in sokoto, nigeria. | rotavirus has been identified among the most important causes of infantile diarrhoea, especially in developing countries. the present study was undertaken to determine the occurrence and clinical symptoms of human rotavirus disease among children presenting with varying degree of diarrhoea in selected urban hospitals in sokoto metropolis, nigeria. diarrhoea samples were collected from 200 diarrheic children younger than 5 years of age and tested using a commercially available dako rotavirus elis ... | 2015 | 26692016 |
| egg yolk igy antibodies: a therapeutic intervention against group a rotavirus in calves. | bovine group a rotavirus (rva) is considered the major cause of diarrhea in intensively reared neonatal calves. chicken egg yolk antibodies (igy) are efficient in protecting neonatal calves from rva diarrhea; however, the value of this intervention in calves once diarrhea has appeared is unclear. the aim of the present study was to evaluate the application of rva-specific igy as a passive treatment in those cases. the experimental groups were: g1=rva-specific igy treatment; g2=no ab treatment; a ... | 2015 | 26679788 |
| detection of avian-like rotavirus a vp4 from a calf in japan. | a total of 568 normal feces from calves on a beef farm in fukui prefecture, japan, in 2011-2012 were examined by rt-semi-nested pcr for rotavirus a (rva) vp4 genes. through partial sequencing and blast analyses of 84 vp4-positive specimens, we identified an avian-like rva strain, n2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. phylogenetic analysis also revealed a close genetic relationship between n2342 and avian rvas, suggest ... | 2015 | 25311984 |
| a bovine g8p[1] group a rotavirus isolated from an asymptomatically infected dog. | group a rotaviruses (rvas) are enteric pathogens with well-documented zoonotic transmissions to humans. the segmented genome of the virus enables reassortment events which might alter host susceptibility and/or disease course. genetic analysis of rotavirus in dogs has so far only revealed rvas with the vp7 and vp4 genome constellation g3p[3]. rva g3p[3] have also been found in cats, humans, monkeys and bats. in the present study, we described an unusual rva of genotype g8p[1] which was isolated ... | 2015 | 25304653 |
| relationship between viral detection and turbidity in a watershed contaminated with group a rotavirus. | enteric viruses are present in aquatic environments due to contamination by raw sewage, even in the absence of fecal coliforms, which are considered to be significant indicators when it comes to microbial water quality assessment. this study investigated the presence of group a rotavirus (rva) in surface water from a river basin in minas gerais, brazil, assessing the influence of the urbanization, the rainfall, and the microbiological and physico-chemical parameters regarding water quality. fort ... | 2015 | 25471713 |
| an outbreak of acute gastroenteritis associated with group a rotavirus in long-term care facility in slovenia. | in april 2013, an outbreak of acute gastroenteritis was reported in a residential long-term care facility (ltcf) in ljubljana. we carried out an outbreak investigation to describe the outbreak, identify factors associated with contracting infection and to recommend control measures. | 2015 | 25447968 |
| molecular epidemiology of rotavirus in cats in the united kingdom. | rotaviruses are leading causes of gastroenteritis in the young of many species. molecular epidemiological studies in children suggest that interspecies transmission contributes to rotavirus strain diversity in people. however, population-based studies of rotaviruses in animals are few. we investigated the prevalence, risk factors for infection, and genetic diversity of rotavirus a in a cross-sectional survey of cats housed within 25 rescue catteries across the united kingdom. morning litter tray ... | 2015 | 25411173 |
| isothermal cross-priming amplification implementation study. | cross-priming amplification (cpa) was evaluated for the early detection of norovirus (nv), rotavirus a (rv-a), enteric adenovirus (eadv) and astrovirus (astv). the analytical sensitivity of the cpa assay was 10(3) copies ml(-1) for nv, rv-a and astv detection and 10(4) copies ml(-1) for eadv detection. for each of the four pathogens, the positive detection rate by cpa was similar to real-time pcr methods and higher than the rate observed in an elisa. the detection coincidence rates of cpa and rt ... | 2015 | 25346035 |
| structural basis for 2'-5'-oligoadenylate binding and enzyme activity of a viral rnase l antagonist. | synthesis of 2'-5'-oligoadenylates (2-5a) by oligoadenylate synthetase (oas) is an important innate cellular response that limits viral replication by activating the latent cellular rnase, rnase l, to degrade single-stranded rna. some rotaviruses and coronaviruses antagonize the oas/rnase l pathway through the activity of an encoded 2h phosphoesterase domain that cleaves 2-5a. these viral 2h phosphoesterases are phylogenetically related to the cellular a kinase anchoring protein 7 (akap7) and sh ... | 2015 | 25878106 |
| crystal structure of the c-terminal 2',5'-phosphodiesterase domain of group a rotavirus protein vp3. | in response to viral infections, the mammalian innate immune system induces the production of the second messenger 2'-5' oligoadenylate (2-5a) to activate latent ribonuclease l (rnase l) that restricts viral replication and promotes apoptosis. a subset of rotaviruses and coronaviruses encode 2',5'-phosphodiesterase enzymes that hydrolyze 2-5a, thereby inhibiting rnase l activation. we report the crystal structure of the 2',5'-phosphodiesterase domain of group a rotavirus protein vp3 at 1.39 å re ... | 2015 | 25758703 |
| multicenter evaluation of the biofire filmarray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. | the appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. additionally, there are limited or no testing met ... | 2015 | 25588652 |
| microbial aetiology of acute diarrhoea in children under five years of age in khartoum, sudan. | diarrhoea is one of leading causes of morbidity and mortality worldwide. recent estimations suggested the number of deaths is close to 2.5 million. this study examined the causative agents of diarrhoea in children under 5 years of age in suburban areas of khartoum, sudan. a total of 437 stool samples obtained from children with diarrhoea were examined by culture and pcr for bacteria, by microscopy and pcr for parasites and by immunoassay for detection of rotavirus a. of the 437 samples analysed, ... | 2015 | 25713206 |
| genotyping and determining the distribution of prevalent g and p types of group a bovine rotaviruses between 2010 and 2012 in iran. | determination and distribution of the g and p genotypes of group a bovine rotavirus was investigated on 386 fecal samples collected from calves with diarrhea using a semi-nested rt-pcr typing assay. samples were collected from 11 provinces of iran during 2010-2012. the provinces divided into 5 different groups based on geographical distance and climates. one hundred and nine samples (28.2%) were confirmed positive for rotavirus group a using elisa. 75 positive samples were selected randomly and ... | 2015 | 26072368 |
| norovirus and rotavirus survival in urine collected from a public ecological sanitation system in ouagadougou, burkina faso. | urine from urine-diversion toilets (udts) is routinely used as fertilizer for urban agriculture in ouagadougou, burkina faso. because urine from udts can be accidentally spoiled by feces, we determined whether virulent enteric viruses could persist in urine that is used for agricultural purposes and pose a threat to human health. urine samples (n = 60) were first collected from 42 udts during the months of january and february 2012 in ouagadougou and screened negative for the presence of norovir ... | 2015 | 25408534 |
| phylogenetic analysis of a g6p[5] bovine rotavirus strain isolated in a neonatal diarrhea outbreak in a beef cattle herd vaccinated with g6p[1] and g10p[11] genotypes. | the aim of this study was to perform the molecular characterization of the eleven genes of a g6p[5] bovine group a rotavirus (rva) strain detected in a diarrhea outbreak from a vaccinated beef cattle herd. the outbreak affected 80 % of calves between 15-30 days old. rva was identified by rt-pcr in 12 (70.6 %) out of 17 diarrheic fecal samples evaluated. the rotavirus wild-type strain had the genotype constellation g6(iv)-p[5](ix)-i2c-r2-c2-m2-a3-n2-t6-e2e-h3a. this study confirms the importance ... | 2015 | 25377636 |
| [the application and epidemiological research of xtag gpp multiplex pcr in the diagnosis of infectious diarrhea]. | to investigate the application value of xtag (®) gastrointestinal pathogen panel (xtag9(®) gpp) multiplex pcr in the early diagnosis of infectious diarrhea, and understand the epidemiology of intestinal diarrhea pathogens. | 2015 | 25916780 |
| full-genome sequencing of a hungarian canine g3p[3] rotavirus a strain reveals high genetic relatedness with a historic italian human strain. | a canine rotavirus a strain was identified in the fecal specimen of a young dog during 2012 in hungary. the strain rva/dog-wt/hun/135/2012/g3p[3] shared complete genotype constellation (g3-p[3]-i3-r3-c3-m3-a15-n2-t3-e3-h6) and high genome sequence similarity (nt, 98.8 %) with a historic human strain, rva/human-tc/ita/pa260-97/1997/g3p[3]. this study provides evidence for the canine origin of the unusual nsp1 genotype, a15, and reinforces the hypothesis of direct interspecies transmission of cani ... | 2015 | 25634124 |
| whole genotype constellation of prototype feline rotavirus strains frv-1 and frv64 and their phylogenetic relationships with feline-like human rotavirus strains. | feline rotaviruses, members of the species rotavirus a, are an infrequent source of zoonotic infections, and were previously shown by rna-rna hybridization assays to possess two distinct genomic rna constellations, represented by strains frv-1 and frv64. due to the lack of whole genome sequence information for frv-1, human rotavirus strain au-1 has been used as a surrogate for the genotype constellation of feline rotaviruses. the aim of this study was to determine the whole genome sequence of fr ... | 2015 | 25351516 |
| prevalence of endemic enteropathogens of calves in new zealand dairy farms. | to conduct a country-wide prevalence study of bovine group a rotavirus, coronavirus, cryptosporidium parvum, salmonella spp. and enterotoxigenic k99(+) escherichia coli (k99) in calves on new zealand dairy farms. | 2015 | 25237728 |
| generation of an avian-mammalian rotavirus reassortant by using a helper virus-dependent reverse genetics system. | the genetic diversity of rotavirus a (rva) strains is facilitated in part by genetic reassortment. although this process of genome segment exchange has been reported frequently among mammalian rvas, it remained unknown if mammalian rvas also could package genome segments from avian rva strains. we generated a simian rva strain sa11 reassortant containing the vp4 gene of chicken rva strain 02v0002g3. to achieve this, we transfected bsr5/t7 cells with a t7 polymerase-driven vp4-encoding plasmid, i ... | 2015 | 26581988 |
| two-year monitoring of water samples from dam of iskar and the black sea, bulgaria, by molecular analysis: focus on mycobacterium spp. | the coast of the bulgarian black sea is a popular summer holiday destination. the dam of iskar is the largest artificial dam in bulgaria, with a capacity of 675 million m3. it is the main source of tap water for the capital sofia and for irrigating the surrounding valley. there is a close relationship between the quality of aquatic ecosystems and human health as many infections are waterborne. rapid molecular methods for the analysis of highly pathogenic bacteria have been developed for monitori ... | 2015 | 26133133 |
| aetiology of acute paediatric gastroenteritis in bulgaria during summer months: prevalence of viral infections. | paediatric acute gastroenteritis is a global public health problem. comprehensive laboratory investigation for viral, bacterial and parasitic agents is helpful for improving management of acute gastroenteritis in health care settings and for monitoring and controlling the spread of these infections. our study aimed to investigate the role of various pathogens in infantile diarrhoea in bulgaria outside the classical winter epidemics of rotavirus and norovirus. stool samples from 115 hospitalized ... | 2015 | 25596126 |
| rapid and simultaneous detection of three major diarrhea-causing viruses by multiplex real-time nucleic acid sequence-based amplification. | rotaviruses, noroviruses and astroviruses are the major viral pathogens leading to diarrhea worldwide. epidemiological investigations of outbreaks associated with these viruses have been impeded by the lack of methods for quick diagnosis and detection. in the current study, a multiplex real-time nucleic acid sequence-based amplification (rt-nasba) system was developed for the simultaneous detection of rotavirus a/norovirus genogroup ii/astrovirus. the specificity and sensitivity of the assay wer ... | 2015 | 25559674 |
| evaluation of molecular- and culture-dependent mst markers to detect fecal contamination and indicate viral presence in good quality groundwater. | microbial contamination of groundwater represents a significant health risk to resource users. culture-dependent bacteroides phage and molecular-dependent bacteroidales 16s rrna assays are employed in microbial source tracking (mst) studies globally, however little is known regarding how these important groups relate to each other in the environment and which is more suitable to indicate the presence of waterborne fecal pollution and human enteric viruses. this study addresses this knowledge gap ... | 2015 | 25871525 |
| detection and molecular characterization of zoonotic viruses in swine fecal samples in italian pig herds. | gastrointestinal disease is frequent in pigs, and among the different etiological agents involved, viruses are considered the leading cause of infection in this animal species. furthermore, about half of the newly identified swine pathogens are viruses, many of which may be transmitted to humans by direct contact or by indirect transmission pathways. in this study, the prevalence of astrovirus (astv), group a rotavirus (rva), norovirus (nov) and hepatitis e virus (hev) infections in pigs was inv ... | 2015 | 26215443 |
| molecular epidemiology of enteric viruses and genotyping of rotavirus a, adenovirus and astrovirus among children under 5 years old in gabon. | this study aimed to determine the prevalence of enteric viruses causing gastroenteritis, and the circulating stains, in gabonese children under five years old who visited health centers between march 2010 and june 2011. | 2015 | 25796432 |
| human and animal enteric virus in groundwater from deep wells, and recreational and network water. | this study was designed to assess the presence of human adenovirus (hadv), rotavirus-a (rva), hepatitis a virus (hav), and porcine circovirus-2 (pcv2) in groundwater from deep wells, and recreational and network waters. the water samples were collected and concentrated and the virus genomes were assessed and quantified by quantitative pcr (qpcr). infectious hadv was evaluated in groundwater and network water samples by integrated cell culture using transcribed messenger rna (mrna) (icc-rt-qpcr). ... | 2015 | 26300358 |
| detection and characterisation of novel bocavirus (genus bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in ireland. | livestock animals have been the assumed source of several human epidemics in recent years, for example, influenza h1n1, rotavirus g8/g9, and mers-cov. surveillance of novel viruses in animals is essential to evaluate the risk to human and animal health and to determine any economic impact, for example, failure to thrive. there is a paucity of data regarding detection and characterisation of gastroenteritis viruses, particularly novel viruses, in porcines in ireland. recently, a number of small n ... | 2015 | 26065833 |
| comparative in vitro and in vivo studies of porcine rotavirus g9p[13] and human rotavirus wa g1p[8]. | the changing epidemiology of group a rotavirus (rv) strains in humans and swine, including emerging g9 strains, poses new challenges to current vaccines. in this study, we comparatively assessed the pathogenesis of porcine rv (prv) g9p[13] and evaluated the short-term cross-protection between this strain and human rv (hrv) wa g1p[8] in gnotobiotic pigs. complete genome sequencing demonstrated that prv g9p[13] possessed a human-like g9 vp7 genotype but shared higher overall nucleotide identity wi ... | 2015 | 26468523 |
| whole genomic analysis of a porcine g6p[13] rotavirus strain. | we report here the whole genomic analysis of an unusual porcine g6p[13] rotavirus-a (rva) strain, rva/pig-wt/ind/hp113/2002/g6p[13], detected from a diarrheic piglet in eastern india in 2002. strain hp113 exhibited a g6-p[13]-i2-r1-c1-m1-a1-n1-t7-e1-h1 genotype constellation, not reported previously. the vp1, vp3, vp4, nsp1 and nsp3-nsp5 genes of rva strain hp113 were found to be closely related to those of porcine and/or porcine-like human rvas. on the other hand, the nsp2 gene of hp113 was fou ... | 2015 | 26454565 |
| rotavirus associated gastroenteritis in thailand. | group a rotavirus is the leading cause of severe diarrhea in infants and young children, and in young animals of many species worldwide. rotavirus is also the major cause of deaths of children younger than 5 years of age, particularly, in developing countries in asia and africa. in thailand, the burden of rotavirus infection rate in children admitted to the hospitals with acute gastroenteritis ranged from 28.4 to 44.5 %. the seasonality of rotavirus gastroenteritis in thailand was detected all-y ... | 2014 | 25674586 |
| rapid detection and high occurrence of porcine rotavirus a, b, and c by rt-qpcr in diagnostic samples. | rotaviruses are important cause of diarrhea in animals, including humans. currently, rotavirus species a, b, c, e, and h (rva-rvc, rve, and rvh) have been identified in pigs. traditionally, rva has been considered the primary cause of diarrhea in pigs, and rvb and rvc had been described sporadically in pigs until recently. qualitative porcine rva, rvb, and rvc rt-pcr (rt-qpcr) assays were designed and 7508 porcine diarrheic samples, submitted to university of minnesota, were tested to estimate t ... | 2014 | 25194889 |
| genetic diversity of porcine group a rotavirus strains in the uk. | rotavirus is endemic in pig farms where it causes a loss in production. this study is the first to characterise porcine rotavirus circulating in uk pigs. samples from diarrheic pigs with rotavirus enteritis obtained between 2010 and 2012 were genotyped in order to determine the diversity of group a rotavirus (garv) in uk pigs. a wide range of rotavirus genotypes were identified in uk pigs: six g types (vp7); g2, g3, g4, g5, g9 and g11 and six p types (vp4); p[6], p[7], p[8], p[13], p[23], and p[ ... | 2014 | 25123085 |
| alterations in oxidant/antioxidant balance, high-mobility group box 1 protein and acute phase response in cross-bred suckling piglets suffering from rotaviral enteritis. | rotaviral enteritis has emerged as a major cause of morbidity and mortality in piglets during their post-natal life. the present study was carried out to examine high-mobility group box 1 (hmgb1) protein, acute phase response and oxidative stress indices in the serum of suckling piglets suffering from enteritis with or without association of porcine group a rotavirus infection. the present investigation utilized 23 clinical cases with signs of acute enteritis and 12 more healthy piglets of a sim ... | 2014 | 24848720 |
| full-length genomic analysis of porcine rotavirus strains isolated from pigs with diarrhea in northern italy. | group a rotaviruses (rva) cause acute dehydrating diarrhea in young of man and many animal species, including pigs. swine rva has an important economic impact on the farming industry, and pigs represent a potential reservoir for zoonotic transmission of rva to humans. to investigate the genetic diversity of porcine rva strains in italy and identify their possible zoonotic characteristics, 25 rva-positive feces were collected from diarrheic pigs in northern italy, in 2009-2010; all viral strains ... | 2014 | 24704759 |
| utility of specific biomarkers to assess safety of swine manure for biofertilizing purposes. | swine production is an important economic activity in brazil, and there is interest in the development of clean production mechanisms to support sustainable agro-industrial activities. the biomass derived from swine manure has good potential to be used as a biofertilizer due to its high nutrient concentration. however, the land application of manure should be based on safety parameters such as the presence of pathogens that can potentially infect animals and people. this study was designed to as ... | 2014 | 24565860 |
| assessment of gastroenteric viruses from wastewater directly discharged into uruguay river, uruguay. | the aim of this study was to assess the viral contamination of group a rotavirus (rva), norovirus (nov), and human astrovirus (hastv) in sewage directly discharged into uruguay river and to characterize rva genotypes circulating in uruguay. for this purpose, sewage samples (n = 96) were collected biweekly from march 2011 to february 2012 in four uruguayan cities: bella unión, salto, paysandú, and fray bentos. each sample was concentrated by ultracentrifugation method. qualitative and quantitativ ... | 2014 | 24777819 |
| molecular epidemiologic trends of diarrhea-causing virus infection from clinical specimens in cheonan, korea, in 2010-2012. | the enteric viruses have high health significance in humans, ranging from poliomyelitis, hepatitis, and gastroenteritis to innocuous infections, and the human enteric viral infection is the one of the most common diseases during early childhood. this study was conducted from july 2010 to october 2012. | 2014 | 24375926 |
| screening of viral pathogens from pediatric ileal tissue samples after vaccination. | in 2010, researchers reported that the two us-licensed rotavirus vaccines contained dna or dna fragments from porcine circovirus (pcv). although pcv, a common virus among pigs, is not thought to cause illness in humans, these findings raised several safety concerns. in this study, we sought to determine whether viruses, including pcv, could be detected in ileal tissue samples of children vaccinated with one of the two rotavirus vaccines. a broad spectrum, novel dna detection technology, the lawr ... | 2014 | 24778651 |
| host-pathogen co-evolution and glycan interactions. | noroviruses and rotavirus a bind to polymorphic glycans of the histo-blood group type (hbgas). norovirus strains that bind to hbgas can collectively infect all humans but each strain only infects a subgroup of the population, suggesting a past co-evolution that led to a trade-off where the human population is partly protected whilst the virus circulation is maintained. we termed 'herd innate protection' the host species partial protection provided by the hbgas polymorphism. given its recent emer ... | 2014 | 25000207 |
| assessment of water quality in a border region between the atlantic forest and an urbanised area in rio de janeiro, brazil. | the preservation of water resources is one of the goals of the designation of parks that act as natural reservoirs. in order to assess the impact of the presence of humans in an environmental preservation area bordering urban areas, the presence of four pathogenic enteric viruses [group a rotavirus (rv-a), norovirus (nov), human adenoviruses (hadv), and hepatitis a virus (hav)], as well as the physico-chemical parameters, and escherichia coli levels were assessed in riverine water samples. from ... | 2014 | 24818567 |
| group a rotavirus and bacterial agents associated with diarrhoea-induced hospitalisations in children below 5 years of age in jammu. | out of 210 faecal samples collected from children below 5 years attending different hospitals in jammu and exhibiting clinical signs of diarrhoea, 41.9% samples were found positive for group a rotavirus by rna-page. escherichia coli isolated in the study belonged to nine serogroups, out of which o69 was most frequent, being present in 12.38% samples. e. coli serogroups well recognised as enteropathogens viz. o69, o20 and o153 were present in 27.6% samples. other bacterial pathogens associated wi ... | 2014 | 24399390 |
| detection and characterization of potentially zoonotic viruses in faeces of pigs at slaughter in germany. | pigs can harbour a variety of viruses in their gastrointestinal tract. some of them are closely related to human viruses and are therefore suspected to have a zoonotic potential. only little is known about the presence of those viruses in pigs at slaughter. however, by contamination of meat with zoonotic viruses during the slaughtering process, food-borne transmission to humans may be possible. here we analyzed 120 faecal samples of pigs at slaughter from 3 different geographical regions of germ ... | 2014 | 24247020 |
| prevalence of rotavirus (garv) and coronavirus (bcov) associated with neonatal diarrhea in calves in western algeria. | to study the prevalence of bovine group a rotavirus (garv) and bovine coronavirus (bcov) in diarrheic feces from calves and the sensitive's parameters such as age group and sex. | 2014 | 25183104 |
| simultaneous concentration of bovine viruses and agricultural zoonotic bacteria from water using sodocalcic glass wool filters. | infiltration and runoff from manured agricultural fields can result in livestock pathogens reaching groundwater and surface waters. here, we measured the effectiveness of glass wool filters to simultaneously concentrate enteric viruses and bacteria of bovine origin from water. the recovery efficiencies were determined for bovine viral diarrhea virus types 1 and 2, bovine rotavirus group a, bovine coronavirus, poliovirus sabin iii, toxigenic escherichia coli ,and campylobacter jejuni seeded into ... | 2014 | 25059211 |
| classification and characterization of a laboratory chicken rotavirus strain carrying g7p[35] neutralization antigens on the genotype 4 backbone gene configuration. | the laboratory rotavirus strain, brs/115, has been used for more than two decades to monitor rotaviruses in specific pathogen free flocks of laying hens. however, the virus strain has not been characterized in detail. therefore we aimed at the description of molecular features of brs115 by using random primed reverse transcription-pcr of the genomic rna followed by massive parallel sequencing using the semiconductor sequencing technology. over 64,000 trimmed reads mapped to reference sequences o ... | 2014 | 25284347 |
| the effect of new neonatal porcine diarrhoea syndrome (nnpds) on average daily gain and mortality in 4 danish pig herds. | the study evaluated the effect of new neonatal porcine diarrhoea syndrome (nnpds) on average daily gain (adg) and mortality and described the clinical manifestations in four herds suffering from the syndrome. nnpds is a diarrhoeic syndrome affecting piglets within the first week of life, which is not caused by enterotoxigenic escherichia coli (etec), clostridium perfringens (c. perfringens) type a/c, clostridium difficile (c. difficile), rotavirus a, coronavirus, cystoisospora suis, strongyloide ... | 2014 | 24755093 |
| evaluation of a multiplex pcr assay for simultaneous detection of bacterial and viral enteropathogens in stool samples of paediatric patients. | we evaluated a multiplex pcr assay, the seeplex diarrhoea ace detection, that simultaneously detects 15 enteric pathogens, including salmonella spp., shigella spp., vibrio spp., toxin b producer clostridium difficile, campylobacter spp., clostridium perfringens, yersinia enterocolitica, aeromonas spp., escherichia coli o157:h7, verocytotoxin-producing escherichia coli, adenovirus, group a rotavirus, norovirus gi and gii, and astrovirus. we compared this assay with clinical methods routinely used ... | 2014 | 24656922 |
| improved detection of gastrointestinal pathogens using generalised sample processing and amplification panels. | we aimed to streamline the diagnosis of gastrointestinal disease by producing multiplexed real time polymerase chain reaction (pcr) panels employing universal sample processing for dna and rna containing pathogens. a total of 487 stored, previously characterised stool samples comprising bacterial, viral, protozoan and clostridium difficile positive samples were tested using four multiplexed real time pcr panels. a further 81 pre-selected clinical samples from a teaching hospital were included to ... | 2014 | 24300711 |
| added value of multiplex luminex gastrointestinal pathogen panel (xtag® gpp) testing in the diagnosis of infectious gastroenteritis. | the luminex gastrointestinal pathogen panel (xtag(®) gpp) detects in one assay the most common gastroenteritis-causing pathogens and toxins, namely adenovirus 40/41, norovirus genogroup (ng) i/ii, rotavirus a, clostridium difficile toxin a/b, campylobacter sp., escherichia coli o157, enterotoxigenic e. coli heat-labile enterotoxin/heat-stable enterotoxin, salmonella sp., shiga-toxin producing e. coli, shiga-like toxin (stx)1/2, shigella sp., vibrio cholerae, yersinia enterocolitica, cryptosporid ... | 2014 | 24131399 |
| fluorescence in situ hybridization investigation of potentially pathogenic bacteria involved in neonatal porcine diarrhea. | neonatal diarrhea is a multifactorial condition commonly present on pig farms and leads to economic losses due to increased morbidity and mortality of piglets. immature immune system and lack of fully established microbiota at birth predispose neonatal piglets to infection with enteric pathogens. the microorganisms that for decades have been associated with enteritis and diarrhea in suckling piglets are: rotavirus a, coronavirus, enterotoxigenic escherichia coli (etec), clostridium perfringens t ... | 2014 | 24628856 |
| genomic characterization of a rotavirus g8p[1] detected in a child with diarrhea reveal direct animal-to-human transmission. | group a rotavirus is a major cause of severe gastroenteritis in children and young animals. during a retrospective analysis of samples collected from paraguayan children under 5 years old with diarrhea, and previously negative for rotavirus and norovirus, we detected the presence of bovine rotavirus sequences by viral metagenomics. nucleic acid was extracted direct from stool sample and determined to be g8p[1]. the genomic analyzes revealed that the strain presents an artiodactyl-like genome (g8 ... | 2014 | 25169054 |
| molecular analysis of non structural rotavirus group a enterotoxin gene of bovine origin from india. | the rotavirus enterotoxin nsp4 (nonstructural protein 4), plays a pivotal role in viral morphogenesis as well as pathogenesis. in this study, the nsp4 gene of rotavirus group a (rva) isolates of bovine origin isolated in several states of india from 2008 to 2011 were characterized. the complete open reading frame of 23 rva strains were sequenced and analyzed phylogenetically. genotype e1 was detected for the first time in bovines from india, in addition to the more common bovine genotype e2. seq ... | 2014 | 24747605 |
| predicted structure and domain organization of rotavirus capping enzyme and innate immune antagonist vp3. | rotaviruses and orbiviruses are nonturreted reoviridae members. the rotavirus vp3 protein is a multifunctional capping enzyme and antagonist of the interferon-induced cellular oligoadenylate synthetase-rnase l pathway. despite mediating important processes, vp3 is the sole protein component of the rotavirus virion whose structure remains unknown. in the current study, we used sequence alignment and homology modeling to identify features common to nonturreted reoviridae capping enzymes and to pre ... | 2014 | 24899176 |
| [serological survey of antibodies against viral diseases of public health interest in llamas (lama glama) from jujuy province, argentina]. | llama population from argentina is mainly concentrated in the andean puna, jujuy. llamas represent an important economic resource for the andean communities. the aim of this study was to investigate the prevalence of antibodies against viral antigens associated to viral diseases of economic impact (neonatal diarrhea, reproductive and respiratory syndromes). a total of 349 serum samples from adult llamas were analyzed. the obtained antibody prevalence was 100 % for rotavirus a and 70 % for bovine ... | 2014 | 24721276 |
| dna microarray for detection of gastrointestinal viruses. | gastroenteritis is a clinical illness of humans and other animals that is characterized by vomiting and diarrhea and caused by a variety of pathogens, including viruses. an increasing number of viral species have been associated with gastroenteritis or have been found in stool samples as new molecular tools have been developed. in this work, a dna microarray capable in theory of parallel detection of more than 100 viral species was developed and tested. initial validation was done with 10 differ ... | 2014 | 25355758 |
| relative abundance and treatment reduction of viruses during wastewater treatment processes--identification of potential viral indicators. | waterborne pathogenic viruses discharged from wastewater treatment plants (wwtps) pose potential public health risks. in the present study, we investigated the relative abundance, occurrence, and reduction of eleven different viruses at two wwtps in southern arizona over a 12-month period, from august 2011 to july 2012. influent and effluent samples from the two wwtps were collected monthly. viruses were concentrated using an electronegative filter method and quantified using taqman-based quanti ... | 2014 | 24836386 |
| [investigation of seven different rna viruses associated with gastroenteritis in children under five years old]. | viruses are the most frequently detected etiologic agents of gastroenteritis seen in small children. in addition to classical gastroenteritis viruses namely rotavirus, norovirus, adenovirus type 40/41, astrovirus and sapovirus, some novel picornaviruses (aichi virus, parechovirus, enterovirus) that have been identified in parallel to the developments in molecular diagnostic methods, thought to be associated with diarrhea in humans. however, the data are not enough to prove their actual roles in ... | 2014 | 24819261 |
| simultaneous detection of five enteric viruses associated with gastroenteritis by use of a pcr assay: a single real-time multiplex reaction and its clinical application. | we developed a highly sensitive reverse transcription and multiplex real-time pcr (rtpcr) assay that can identify five viruses, including six genogroups, in a single reaction: norovirus genogroups i and ii; sapovirus genogroups i, ii, iv, and v; human rotavirus a; adenovirus serotypes 40 and 41; and human astrovirus. in comparison to monoplex rtpcr assays, the sensitivities and specificities of the multiplex rtpcr ranged from 75% to 100% and from 99% to 100%, respectively, evaluated on 812 clini ... | 2014 | 24478418 |
| a two-tube multiplex reverse transcription pcr assay for simultaneous detection of viral and bacterial pathogens of infectious diarrhea. | diarrhea caused by viral and bacterial infections is a major health problem in developing countries. the purpose of this study is to develop a two-tube multiplex pcr assay using automatic electrophoresis for simultaneous detection of 13 diarrhea-causative viruses or bacteria, with an intended application in provincial centers for diseases control and prevention, china. the assay was designed to detect rotavirus a, norovirus genogroups gi and gii, human astrovirus, enteric adenoviruses, and human ... | 2014 | 24711998 |
| detection of rare g3p[19] group a rotavirus in human patient, italy. | infection with a rare g3p[19] rotavirus a strain was identified in an immunosuppressed patient in italy. the strain showed a p[19] viral protein 4 gene and a complete au-1-like genomic constellation. phylogenetic analyses showed high nucleotide identity between this strain and g3p[19] rotavirus a strains from asia, indicating possible reassortment events. | 2014 | 25340712 |
| analysis of rotavirus genotypes in korea during 2013: an increase in the g2p[4] genotype after the introduction of rotavirus vaccines. | group a rotavirus is the leading cause of acute gastroenteritis in children worldwide. we investigated g and p genotypes of group a rotavirus strains isolated from patients during 2013 and investigated which genotypes were identified from vaccinated patients. | 2014 | 25312273 |
| acute diarrhea in adults consulting a general practitioner in france during winter: incidence, clinical characteristics, management and risk factors. | data describing the epidemiology and management of viral acute diarrhea (ad) in adults are scant. the objective of this study was to identify the incidence, clinical characteristics, management and risk factors of winter viral ad in adults. | 2014 | 25358721 |
| rotavirus infection in children and adults with acute gastroenteritis in thailand. | and young children, but rotavirus gastroenteritis in adults is uncommon. in this study, 260 stool samples collected in thailand from january 2006 to february 2007 from patients, of all ages with acute gastroenteritis, were tested for group a rotavirus and compared with rotavirus infections in children and adults. rota- virus was detected in 42% of the patients' samples, but children (< 18 years old) have a significantly higher prevalence (57%) of rotavirus infection than adults (≥ 18 years old) ... | 2014 | 25427349 |
| full genome characterization of human rotavirus a strains isolated in cameroon, 2010-2011: diverse combinations of the g and p genes and lack of reassortment of the backbone genes. | over the past few years whole genome sequencing of rotaviruses has become a routine laboratory method in many strain surveillance studies. to study the molecular evolutionary pattern of representative cameroonian rotavirus a (rva) strains, the semiconductor sequencing approach was used following random amplification of genomic rna. in total, 31 rva strains collected during 2010-2011 in three cameroonian study sites located 120 to 1240 km from each other were sequenced and analyzed. sequence anal ... | 2014 | 25460824 |
| whole genomic analysis of porcine g10p[5] rotavirus strain p343 provides evidence for bovine-to-porcine interspecies transmission. | porcine group a rotavirus (rva) strain p343 (rva/pig-tc/tha/p343/1991/g10p[5]) was suggested to have vp7 and vp4 genes of bovine origin. in order to obtain precise information on the exact origin and evolution of this unusual porcine strain, the remaining nine genes (vp6, vp1-3, and nsp1-5) of strain p343 were sequenced and analyzed in the present study. on whole genomic analysis, strain p343 was found to have a bovine rva-like genotype constellation (g10-p[5]-i2-r2-c2-m2-a3-n2-t6-e2-h3) differe ... | 2014 | 25457370 |
| diversity of rotavirus strains causing diarrhea in <5 years old chinese children: a systematic review. | we conducted a systematic review of the diversity and fluctuation of group a rotavirus strains circulating in china. | 2014 | 24416267 |
| detection and genetic characterization of human enteric viruses in oyster-associated gastroenteritis outbreaks between 2001 and 2012 in osaka city, japan. | enteric viruses are an important cause of viral food-borne disease. shellfish, especially oysters, are well recognized as a source of food-borne diseases, and oyster-associated gastroenteritis outbreaks have on occasion become international occurrences. in this study, 286 fecal specimens from 88 oyster-associated gastroenteritis outbreaks were examined for the presence of 10 human enteric viruses using antigenic or genetic detection methods in order to determine the prevalence of these infection ... | 2014 | 24415518 |
| molecular analysis of group a rotaviruses detected in adults and adolescents with severe acute gastroenteritis in italy in 2012. | hospital-based surveillance of acute gastroenteritis caused by rotavirus has produced ample knowledge on the infection in children, whereas little is known on rotavirus infection among adults. the italian surveillance program rotanet-italia collected 1,595 samples from patients admitted to hospital with gastroenteritis in 2012. all patients presented with vomiting, diarrhea, fever, and/or abdominal pain. forty-two samples obtained by the rotanet-italia (2.6%) were from adolescents or adults (10- ... | 2014 | 24415411 |
| genetic diversity of group a rotavirus in swine in canada. | group a rotaviruses (rva) in pigs have been poorly investigated in canada. in a continued effort to fill this gap, ten finisher swine farms in quebec, canada, were sampled over a nine-month period. the presence of rva was detected in healthy pigs on all farms investigated during the entire sampling period. the genotypes detected included g2, g5, g9 and g11; p[6], p[7], p[13], p[27] and p[34]; and i5 and i14. the predominant types were g2, p[13] and i5, which is different from previous global rep ... | 2014 | 24402632 |
| epidemiology of rotavirus diarrhea and diversity of rotavirus strains among children less than 5 years of age with acute gastroenteritis in mauritius: june 2008 to december 2010. | rotavirus is an important etiologic agent of diarrhea worldwide and ongoing surveillance is essential to monitor strain diversity. to describe the epidemiology of rotavirus disease and circulating rotavirus strains in mauritius, surveillance for rotavirus diarrhea was conducted at 2 regional hospitals from june 2008 through december 2010. | 2014 | 24343614 |
| hospital-based surveillance for rotavirus gastroenteritis in children younger than 5 years of age in ethiopia: 2007-2012. | rotavirus surveillance was initiated in ethiopia to estimate the burden of rotavirus gastroenteritis in children <5 years of age, to generate data to assist the policy-making process for new vaccine introduction and to monitor impact of vaccination on disease burden after introduction. | 2014 | 24343610 |
| real-time rt-pcr assays to differentiate wild-type group a rotavirus strains from rotarix(®) and rotateq(®) vaccine strains in stool samples. | group a rotaviruses (rva) are the leading cause of severe diarrhea in young children worldwide. two live-attenuated rva vaccines, rotarix(®) and rotateq(®) are recommended by world health organization (who) for routine immunization of all infants. rotarix(®) and rotateq(®) vaccines have substantially reduced rva associated mortality but occasionally have been associated with acute gastroenteritis (age) cases identified in vaccinees and their contacts. high-throughput assays are needed to monitor ... | 2014 | 24342877 |
| complete genotype constellation of human rotavirus group a circulating in thailand, 2008-2011. | this study has identified diverse and re-assorted group a rotavirus (rva) strains by sequence and phylogenetic analysis of the 11 genomic segments. the 22 cases investigated in this study were collected from children with diarrhea between 2008 and 2011. the rva genomic constellations identified in this study were identified as g1-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1 22.7% (5/22); g2-p[4]-i2-r2-c2-m2-a2-n2-t2-e2-h2 27.3% (6/22); g3-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1 18.2% (4/22); g3-p[9]-i3-r3-c3-m3-a3-n ... | 2014 | 24296010 |
| a fut2 gene common polymorphism determines resistance to rotavirus a of the p[8] genotype. | attachment to carbohydrates of the histo-blood group type of several human rotavirus strains (rva) has recently been described. synthesis of these ligands requires a functional fut2 enzyme, suggesting that fut2 null homozygote (ie, nonsecretor) individuals may not be recognized by most human rva strains. whereas such individuals represent 20% of the control population, this retrospective study determined that none of 51 patients infected by p[8] rotavirus strains were nonsecretors. the lack of α ... | 2014 | 24277741 |
| g8p[6] rotaviruses isolated from amerindian children in mato grosso do sul, brazil, during 2009: close relationship of the g and p genes with those of bovine and bat strains. | during the 2009 national group a rotavirus (rva) surveillance, five unusual strains of the human g8p[6] genotype were detected in brazilian indian children with acute gastroenteritis. the aim of this study was to carry out sequence analysis of the two outer capsid proteins (vp4 and vp7) and the inner capsid protein (vp6) of the g8p[6] strains detected in order to provide further information on the genetic relationship between human and animal rva. a total of 68 stool samples, collected in mato g ... | 2014 | 24259191 |
| [evaluation of real-time multiplex pcr effectiveness for group a rotavirus genotyping]. | evaluate resolution and diagnostic significance of real-time multiplex pcr (mp rt-pcr) as a platform for group a rotavirus g/p genotyping test-systems. | 2014 | 25286527 |
| [evolutionary relationships of g3 garv isolated from pigs and humans in lulong county, hebei province, china]. | this study aimed to amplify major genome segments (vp7, vp4, vp6, vp2 and nsp2-5) of porcine g3 group a rotavirus (garv) llz212 isolated in our laboratory, determine their genotypes, and explore the evolutionary relationships between g3 garv strains isolated from humans and pigs in lulong county, hebei province, china. major genome segments of seven garv strains were amplified by reverse transcription-polymerase chain reaction (rt-pcr) and the segments were sequenced. the genome segments of seve ... | 2014 | 25272597 |
| prevalence and genomic characterization of g2p[4] group a rotavirus strains during monovalent vaccine introduction in brazil. | this study aims to: estimate the prevalence of g2p[4] rotaviruses in brazil between 2001-2011 from patients with acute gastroenteritis; perform phylogenetic analyses of g2p[4] brazilian strains (from vaccinated and non-vaccinated children) based on vp7 and vp8(∗) encoding genes and analyze the antigenic regions of these proteins comparing with rv1; and assess the full genetic background of eleven selected brazilian strains. the g2p[4] detection rate among rva positive samples was 0/157 in 2001, ... | 2014 | 25239525 |
| a possible packaging signal in the rotavirus genome. | group a rotavirus (rva), an etiological agent of gastroenteritis in young mammals and birds, possesses a genome of 11 double-stranded rna segments. although it is believed that the rva virion contains one copy of each genomic segment and that the positive-strand rna (+rna) is incorporated into the core shell, the packaging mechanisms of rva are not well understood. here, packaging signals of rva were searched for by analyzing genomic sequences of mammalian and avian rva, which are considered to ... | 2014 | 25224974 |
| noroviruses associated with outbreaks of acute gastroenteritis in the state of rio grande do sul, brazil, 2004-2011. | acute gastroenteritis norovirus (nov) in a country of continental dimensions like brazil has resulted in under-reporting of the number of outbreaks, as well as the genotypes associated. | 2014 | 25223919 |