Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| assessment of the genetic diversity among arcobacters isolated from poultry products by using two pcr-based typing methods. | in this study, enterobacterial repetitive intergenic consensus pcr (eric-pcr) and randomly amplified polymorphic dna pcr (rapd-pcr) were optimized for characterization of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii. in addition, a simple and rapid dna extraction method was tested for use in both typing procedures. both methods had satisfactory typeability and discriminatory power, but the fingerprints generated with eric-pcr were more reproducible and complex than tho ... | 2002 | 11976086 |
| identification by 16s ribosomal rna gene sequencing of arcobacter butzleri bacteraemia in a patient with acute gangrenous appendicitis. | to identify a strain of gram negative facultative anaerobic curved bacillus, concomitantly isolated with escherichia coli and streptococcus milleri, from the blood culture of a 69 year old woman with acute gangrenous appendicitis. the literature on arcobacter bacteraemia and arcobacter infections associated with appendicitis was reviewed. | 2002 | 12032229 |
| discrimination of arcobacter butzleri isolates by polymerase chain reaction-mediated dna fingerprinting. | the objective of this study was to subtype arcobacter butzleri isolates using rapd-pcr. | 2002 | 12100590 |
| occurrence and distribution of arcobacter species in poultry processing. | a total of 16 broiler flocks slaughtered in the morning in eight belgian poultry slaughterhouses were examined for the presence of campylobacteraceae. in samples collected before and after chilling, the prevalence of arcobacters was found to be higher than the prevalence of thermophilic campylobacters, with the slaughter procedure used having no clear effect. two slaughterhouses were selected for a detailed investigation of the occurrence and distribution of arcobacters. sampling carried out bef ... | 2002 | 12182473 |
| attachment of arcobacter butzleri, a new waterborne pathogen, to water distribution pipe surfaces. | the capability of arcobacter butzleri to attach to various water distribution pipe surfaces, such as stainless steel, copper, and plastic, was evaluated using scanning electron microscopy. our results indicated that arcobacter cells could easily attach to all surface types and the number of attached cells depended on the length of exposure and temperatures (4 and 20 degrees c). extracellular fibrils were also observed on the stainless steel surface, especially after 72 h of contact times at both ... | 2002 | 12182474 |
| susceptibility of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii to antimicrobial agents used in selective media. | several antimicrobial agents used in selective media for the isolation of arcobacter were found to be inhibitory to strains belonging to this genus. all three species tested were susceptible to colistin and rifampin at concentrations used in selective media. arcobacter skirrowii was the most susceptible species. 5-fluorouracil, novobiocin, trimethoprim, and teicoplanin or vancomycin were found to be without any inhibitory effect on the strains tested at concentrations described for the isolation ... | 2001 | 11283110 |
| the recovery of arcobacter butzleri nctc 12481 from various temperature treatments. | the aim of this study was to determine the growth and survival characteristics for arcobacter butzleri nctc 12481. | 2001 | 11722672 |
| susceptibility of arcobacter butzleri isolates to 23 antimicrobial agents. | the objective of this study was to determine the susceptibility of arcobacter butzleri isolates to various antimicrobial agents used in the treatment of infectious diseases in humans and animals. | 2001 | 11737626 |
| development of a new protocol for the isolation and quantification of arcobacter species from poultry products. | none of the presently available selective supplements for the specific isolation of arcobacter species allows the growth of arcobacter butzleri, a. cryaerophilus and a. skirrowii and at the same time fully suppresses the accompanying flora present in poultry and poultry products. furthermore, little is known about the contamination levels of poultry with arcobacter species. in this study, a new selective supplement comprising amphotericin b (10 mg/l), cefoperazone (16 mg/l), 5-fluorouracil (100 ... | 2001 | 11789937 |
| arcobacter butzleri bacteremia in a patient with liver cirrhosis. | invasive arcobacter butzleri infection in humans has been rarely described. we report a 60-year-old man with liver cirrhosis who presented with high fever and esophageal variceal bleeding. two aerobic blood cultures grew campylobacter-like organisms. the biochemical reactions of the isolate were inconclusive, while sequence analysis of the 16s rrna gene definitively identified the organism as a. butzleri. the patient's bleeding stopped after endoscopic sclerosing therapy. although the organism a ... | 2000 | 10770033 |
| multiplex pcr detection of campylobacter jejuni and arcobacter butzleri in food products. | arcobacter is a recently described species, previously considered part of the campylobacter family. a sensitive assay such as that provided by pcr could help to distinguish the closely related arcobacter from campylobacter. a pcr method to specifically detect both campylobacter jejuni and arcobacter butzleri in the same reaction tube has been developed. c. jejuni and a. butzleri were inoculated into a range of dairy products, raw and ready-to-eat foods. the presence of these two organisms was de ... | 2000 | 10799270 |
| isolation of arcobacter spp. from a brackish environment. | arcobacter spp. were isolated from water and mussels of two brackish lakes near messina (italy). the isolates were phenotypically characterized on the basis of a large battery of cultural and biochemical tests. by comparison with the reference strains arcobacter butzleri atcc 49616 and a. cryaerophilus atcc 43157 they may be considered arcobacter butzleri-like bacteria. the current isolation suggests that the brackish environment may play an important role in the survival and transmission of arc ... | 2000 | 10872684 |
| development of a multiplex pcr assay for the simultaneous detection and identification of arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii. | a multiplex pcr assay with five primers targeting the 16s and 23s rrna genes was developed for the simultaneous detection and identification of arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii. the selected primers amplify a 257-bp fragment from a. cryaerophilus, a 401-bp fragment from a. butzleri and a 641-bp fragment from a. skirrowii. no pcr product was generated for closely related bacteria including campylobacter and helicobacter species. the assay was useful to identi ... | 2000 | 11094284 |
| prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. | the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ... | 2000 | 10618103 |
| identification of campylobacter jejuni, c. coli, c. lari, c. upsaliensis, arcobacter butzleri, and a. butzleri-like species based on the glya gene. | currently, the detection and identification of campylobacter and arcobacter species remains arduous, largely due to cross-species phenotypic similarities and a relatively narrow spectrum of biochemical reactivity. we have developed a pcr-hybridization strategy, wherein degenerate primers are used to amplify glya fragments from samples, which are then subjected to species-specific oligodeoxyribonucleotide probe hybridizations, to identify and distinguish between campylobacter jejuni, c. coli, c. ... | 2000 | 10747131 |
| arcobacter butzleri isolated from a diarrhoeic non-human primate. | the bacteriological examination of a faecal specimen from a 20-year-old female rhesus macaque (macaca mulatta) with diarrhoeal illness revealed the presence of a large number of a relatively new enteric pathogen, arcobacter butzleri. the animal was from a closed colony of about 60 females, some of them were showing intermittent diarrhoea possibly related to giardia spp. conditions for the isolation and identification of a. butzleri are reported, as well as discussions about its role as a primary ... | 1999 | 10759398 |
| arcobacter cryaerophilus and arcobacter butzleri isolated from preputial fluid of boars and fattening pigs in brazil. | 1999 | 12968762 | |
| the effect of citric acid, lactic acid, sodium citrate and sodium lactate, alone and in combination with nisin, on the growth of arcobacter butzleri. | the importance of arcobacter spp. as a cause of human foodborne illness is unresolved. organic acids and their sodium salts, and nisin are preservatives commonly used in the type of foods from which the organism is recovered. in this study their effect on the growth of a. butzleri in culture, alone and in combination, was investigated. at 0.5%, 1.0% and 2.0% lactic and citric acids inhibited a. butzleri growth; 2% sodium lactate was effective in inhibiting growth over 8 h incubation but not over ... | 1999 | 10664988 |
| isolation of arcobacter butzleri from ground water. | arcobacter butzleri was isolated from a contaminated ground water source. these organisms, previously designated as aerotolerant campylobacter, were capable of surviving in the ground water environment. specific dna probes were used to characterize the isolates in the initial identification and survival studies. arcobacter butzleri was found to be sensitive to chlorine inactivation. | 1999 | 10030029 |
| natural and experimental infections of arcobacter in poultry. | arcobacter butzleri causes human enteritis and is frequently recovered from poultry carcasses. the purpose of this study was to determine 1) the natural distribution of a. butzleri in poultry and 2) its relative pathogenicity in experimentally infected poultry. cloacal samples (n = 407) were collected on four occasions from three flocks of chickens. overall, arcobacter spp. were recovered from 15% of the birds; a. butzleri was identified in 1% of cloacal samples. three experimental trials were c ... | 1999 | 10230906 |
| occurrence, removal and seasonal variation of thermophilic campylobacters and arcobacter in sewage sludge. | the presence of thermophilic campylobacters and arcobacter was investigated in four types of sewage sludge taken from the treatment plant in bologna (italy): primary, activated, thickened and anaerobically digested sludge. campylobacter jejuni and campylobacter coli were more numerous during the march-september period and were found only in primary sludge (22.7%) with mean counts of 278 mpn/g dry matter and 1403 mpn/g dry matter respectively. arcobacter butzleri were found in all types of sludge ... | 1999 | 10418097 |
| identification and characterization of arcobacter isolates from broilers by biochemical tests, antimicrobial resistance patterns and plasmid analysis. | a total of 89 arcobacter butzleri isolates obtained from freshly slaughtered broilers was investigated for their biochemical capacities by using the api campy system as well as additional growth and tolerance tests, for their antimicrobial resistance patterns and their plasmid content. among these techniques, biochemical profiling and antimicrobial resistance testing proved to be the most discriminatory methods as confirmed by the calculation of discriminatory indices. these two methods might re ... | 1998 | 9557130 |
| isolation of arcobacter butzleri in raw water and drinking water treatment plants in germany. | a total of 147 "campylobacter-like" strains were isolated from six drinking water treatment plants within a two year investigation period. the strains were characterized by bio-, and serotyping according the scheme for arcobacter butzleri. the result was that 100 strains were typed as arcobacter butzleri, 17 strains as arcobacter butzleri-like and 6 strains as campylobacter jejuni/coli. 24 strains were typed as arcobacter spp. the strains isolated from the treatment plants showed the same seroty ... | 1998 | 9686447 |
| diversity and prevalence of arcobacter spp. in broiler chickens. | ninety-nine strains of arcobacter spp., isolated from 10 chicken carcasses purchased from a supermarket and 15 chicken carcasses collected from a poultry abattoir, were speciated using a variety of phenotypic identification methods. all were tested using api campy test strips and the 16-test (preston) identification scheme developed for campylobacters. fifty strains were selected for examination using a more comprehensive probabilistic identification scheme, and the identity of representative st ... | 1998 | 9717285 |
| prevalence and genetic variability of arcobacter species in mechanically separated turkey. | a survey for arcobacter spp. and arcobacter butzleri in mechanically separated turkey was conducted during the winter of 1995 and summer and fall of 1996. arcobacter spp. and a. butzleri were identified by polymerase chain reaction and species-specific oligonucleotide probes. arcobacter spp. were isolated from 77% (303 out of 395) of the mechanically separated turkey samples with 74% (223 out of 303) of these samples positive for a. butzleri. of the 121 a. butzleri isolates tested, 86 different ... | 1998 | 9874339 |
| in vitro virulence factors of arcobacter butzleri strains isolated from superficial water samples. | eighteen isolates of a. butzleri from river water samples were examined for their biotype, serogroup and putative virulence characteristics. toxin profiles based on cytotonic, cytotoxic and cytolethal distending factors were determined after analysis responses in vero and cho cells adhesivity and invasivity tests were performed on hela and intestine 407 cells. six biotypes and five serogroups were determined in our isolates. all strains but one induced cytotoxic effects on cells in culture. the ... | 1997 | 9037670 |
| emerging issues in microbiological food safety. | many microorganisms previously unrecognized as food-borne or harmful are emerging as human pathogens transmitted by food. this is a result of recent acquisition of key virulence factors, detection by newly developed isolation procedures, or astute detective-like laboratory skills of microbiologists. six microbial pathogens, including shiga toxin-producing escherichia coli, listeria monocytogenes, arcobacter butzleri, helicobacter pylori, cryptosporidium parvum, and cyclospora, have become recogn ... | 1997 | 9240928 |
| multiplex pcr for the identification of arcobacter and differentiation of arcobacter butzleri from other arcobacters. | a multiplex polymerase chain reaction (pcr) assay to identify arcobacter isolates and to distinguish a. butzleri from other arcobacters is described. the test uses two primer sets. set i targets a section of the 16s rrna genes of arcobacter spp. set ii amplifies a portion of the 23s rrna genes unique to a. butzleri. specificity of the primer sets was evaluated using atcc reference strains of a. butzleri, a. cryaerophilus, a. skirrowii, bacteroides spp., campylobacter spp., helicobacter spp. and ... | 1997 | 9453132 |
| comparative disinfection of secondary-treated sewage with chlorine dioxide and bromine chloride. | a comparison was made of the inactivation rates of arcobacter butzleri, coliphages, total coliforms, fecal coliforms, fecal streptococci and heterotrophic plate count in secondary sewage effluent using chlorine dioxide (2 and 4 ppm) and bromine chloride (4 or 8 and 12 ppm) as disinfecting agents. using these doses the clo2 gave higher reduction percentages (on average more than 99% at 4 ppm) than those obtained with brcl. the average values of the fecal indicators are well within the legal limit ... | 1996 | 9353543 |
| infection of cesarean-derived colostrum-deprived 1-day-old piglets with arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii. | neonatal piglets have been used as models to study human campylobacteriosis and helicobacteriosis. the purpose of this study was to determine the relative pathogenicities, on the basis of the duration of fecal shedding and colonization of tissues, of three arcobacter species in 1-day-old cesarean-derived colostrum-deprived piglets. two experiments were conducted. in experiment 1, two piglets each were infected per os with either arcobacter butzleri atcc 49616, arcobacter cryaerophilus 1b atcc 43 ... | 1996 | 8675340 |
| phenotypic and ribosomal rna characterization of arcobacter species isolated from porcine aborted fetuses. | aerotolerant organisms resembling campylobacter, now designated as arcobacter, have been described from aborted farm animals and from cases of human enteritis worldwide. the goals of this study were 1) to attempt to recover arcobacter spp. from cases of porcine abortion, 2) to characterize these isolates by phenotype and ribotype, and 3) to compare the usefulness of ribotype and phenotype patterns for identifying arcobacter butzleri and the dna hybridization groups 1a and 1b of a. cryaerophilus. ... | 1996 | 8744740 |
| prevalence of thermophilic campylobacter and arcobacter butzleri in food of animal origin. | thermophilic campylobacters and arcobacter butzleri were looked for in 57 manually shelled egg samples and 130 raw meat samples. no bacteria were found in the egg samples, but they were, however, found in 16.1% of meat samples with a frequency varying from 2.4% (port sausage) to 37.5% (poultry). a total of 21 strains were identified, one belonging to arcobacter butzleri (pork) and 20 to campylobacter. the most common species was c. jejuni (16/20) followed by c. coli (4/20) and in both species bi ... | 1996 | 8930716 |
| pcr-mediated dna fingerprinting of atypical campylobacter strains isolated from surface and drinking water. | this study shows that typing through polymerase chain reaction (pcr) can be used to differentiate between strains of campylobacter and arcobacter, whereas by means of conventional biotyping arcobacter might be identified as atypical campylobacter. microaerophilic "campylobacter-like" organisms were isolated from several drinking water treatment plants as well as environmental sources. the strains were characterized by biotyping and pcr amplification of campylobacter flaa/flab and 16s-rrna arcoba ... | 1996 | 8946702 |
| identification of arcobacter isolates by pcr. | a polymerase chain reaction (pcr) assay was developed for the identification of the three species of arcobacter which have been recovered from clinically ill or healthy humans and/or livestock, namely arcobacter butzleri, arcobacter skirrowii and arcobacter cryaerophilus. the assay utilizes primers targeted to the genes encoding 16s rrna of arcobacter spp. the assay reduces the amount of time required to positively identify strains of arcobacter. | 1996 | 8987697 |
| isolation of arcobacter butzleri from a neonate with bacteraemia. | we describe the case of a neonate with bacteraemia from whom the recently described organism arcobacter butzleri was isolated. this appears to be the first report of the organism causing neonatal infection. clinical details suggest that the infection was contracted in utero, although the mother showed no evidence of disease before delivery. treatment of the preterm infant was ultimately successful in resolving the infection but the organism proved resistant to a wide range of antibiotics. simila ... | 1995 | 8586843 |
| arcobacter-specific and arcobacter butzleri-specific 16s rrna-based dna probes. | the genus arcobacter encompasses gram-negative, aerotolerant, spiral-shaped bacteria formerly designated campylobacter cryaerophila. two genus-specific 16s rrna-based oligonucleotide dna probes (23-mer and 27-mer) were developed. the probes hybridized with strains of arcobacter butzleri (n = 58), arcobacter cryaerophilus (n = 19), and arcobacter skirrowii (n = 17). the probes did not cross-react with any of the reference strains of campylobacter, helicobacter, including "flexispira rappini," or ... | 1995 | 7545177 |
| severe diarrhea associated with arcobacter butzleri. | two patients with chronic disease (diabetes mellitus type i, hyperuricemia and alcohol abuse, respectively) were hospitalized with persistent diarrhea and severe abdominal cramps. using routine methods, the only pathogen isolated in stool specimens was arcobacter butzleri. in both cases acute symptoms subsided quickly after antibiotic therapy. after termination of antibiotic treatment, arcobacter butzleri could no longer be detected in stool specimens. although very little is known about the cli ... | 1994 | 7813498 |
| discrimination of epidemic and sporadic isolates of arcobacter butzleri by polymerase chain reaction-mediated dna fingerprinting. | dna polymorphisms of arcobacter butzleri outbreak-related strains and arcobacter reference strains were determined by use of the polymerase chain reaction with primers aimed at repetitive sequences. the epidemiological relationship among 14 outbreak-related strains was substantiated, as they showed virtually no genomic variations. their dna amplification patterns were, however, clearly different from those of all arcobacter reference strains studied; each reference strain was characterized by a ... | 1993 | 8308127 |
| isolation of arcobacter butzleri from a drinking water reservoir in eastern germany. | microaerophilic "campylobacter-like organisms" (clo) were isolated from a drinking water reservoir in germany during a period of some months. the strains were characterized by biotyping (api campy), serotyping and sds-disc-gelelectrophoresis as arcobacter butzleri. now this facultative humanpathogen species was first described in germany. with respect to the drinking water treatment, the hygienic importance of the findings is given. | 1993 | 8318136 |
| arcobacter (campylobacter) butzleri-associated diarrheal illness in a nonhuman primate population. | after dna hybridization identified an isolate from an ill rhesus macaque (macaca mulatta) as arcobacter (campylobacter) butzleri, we initiated a study to determine whether a. butzleri was associated with diarrheal disease in nonhuman primates at the yerkes primate research center. by using campy-cva medium incubated at 35 degrees c, 15 a. butzleri isolates were obtained from 14 macaques; 7 macaques were coinfected with campylobacter coli and campylobacter jejuni. a. butzleri was not isolated fro ... | 1993 | 8478115 |
| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| outbreak of recurrent abdominal cramps associated with arcobacter butzleri in an italian school. | in the autumn of 1983, an outbreak of recurrent abdominal cramps occurred in a nursery and primary school in the rovigo area in italy. none of the 10 affected children had diarrhea. an atypical campylobacter-like organism was isolated from feces in all cases. conventional enteropathogens were searched for but not detected. the campylobacter-like organism was identified as arcobacter butzleri by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins and cellular fa ... | 1992 | 1400998 |
| polyphasic taxonomic study of the emended genus arcobacter with arcobacter butzleri comb. nov. and arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. | the relationships of 77 aerotolerant arcobacter strains that were originally identified as campylobacter cryaerophila (now arcobacter cryaerophilus [p. vandamme, e. falsen, r. rossau, b. hoste, p. segers, r. tytgat, and j. de ley, int. j. syst. bacteriol. 41:88-103, 1991]) and 6 reference strains belonging to the taxa arcobacter nitrofigilis, arcobacter cryaerophilus, and "campylobacter butzleri" were studied by using a polyphasic approach, in which we performed dna-rrna hybridizations, dna-dna ... | 1992 | 1503968 |
| campylobacter butzleri sp. nov. isolated from humans and animals with diarrheal illness. | seventy-eight aerotolerant campylobacter isolates were characterized phenotypically and by dna hybridization (hydroxyapatite method at 50 and 65 degrees c). two dna relatedness groups were found. (i) sixty-four strains belonged to aerotolerant campylobacter dna hybridization group 2. these organisms were isolated from humans, primarily with diarrheal illness, and animals on several continents. strains were aerotolerant at 30 and 36 degrees c and catalase negative or weakly catalase positive, gre ... | 1991 | 2007646 |
| isolation of group 2 aerotolerant campylobacter species from thai children with diarrhea. | campylobacter species were isolated from 93 (15%) of 631 thai children with diarrhea using the membrane filter technique on nonselective blood agar incubated at 37 degrees c. campylobacter jejuni was isolated from 62 (10%), campylobacter coli from 14 (2%), and atypical campylobacters from 17 (3%). the 17 atypical strains were first characterized biochemically and by dot blot dna hybridization. catalase-negative strains also were characterized by dna hybridization and ribotype pattern. one strain ... | 1991 | 2019754 |