Publications

detection of genomic-length soybean mosaic virus rna on polyribosomes of infected soybean leaves.	soybean mosaic virus (smv)-related rnas were examined in both polyribosomal and nonpolyribosomal fractions of systemically infected soybean leaves. viral rnas were detected by northern blot hybridization analysis using two cloned smv-cdnas representing different regions of the viral genome as hybridization probes. genomic length smv-rna (mr of 3.3 x 10(6] was found in specific association with edta-sensitive polyribosomes of infected leaves, indicating that it functions as a messenger rna in the ...	1984	6388148
rna-protein interactions in some small plant viruses.	the structure of the three quasi-equivalent protein subunits a, b and c of the spherical, t = 3 southern bean mosaic virus (sbmv) have been carefully built in accordance with a refined electron density map of the complete virus. the lower electron density in the rna portion of the map could not be explicitly interpreted in terms of a preferred rna structure on which some icosahedral symmetry might have been imposed. however, the extremely basic nature of the interior surface of the coat protein ...	1983	6401119
the self-assembly of the cowpea strain of southern bean mosaic virus: formation of t = 1 and t = 3 nucleoprotein particles.	virion-like particles of the cowpea strain of southern bean mosaic virus were assembled from isolated coat protein and rna components in vitro at low ionic strength. purified southern bean mosaic virus rna was heterogeneous on sucrose gradients, and was separated into low (0.3 - 0.6 x 10(6) da) and high (1.0-1.4 x 10(6) da) molecular weight (mw) fractions. assembly with high mw rna resulted in t = 3 particles at ph 7 and 9. low mw rna assembled with coat protein into t = 1 particles at ph 5 and ...	1983	18638938
carbon-13 nmr study of southern bean mosaic virus.	the motional state of the coat protein of southern bean mosaic virus (sbmv) particles has been studied by 13c nmr at 50.3 mhz. in the native virus, 13c-nmr signals are observed only from a few highly mobile amino acid side chain groups such as methyls or from unprotonated carbons such as carbonyls or arginine-zeta. after swelling sbmv by treating with edta, additional 13c-nmr peaks become visible. the pattern of resonances matches that expected from the side chains of those amino acid residues i ...	1982	18635125
intermediates of the in vitro assembly and disassembly of southern bean mosaic virus.	southern bean mosaic virus (sbmv) was swollen by treatment with edta at ph 7.5 and dissociated into rna and protein in 1 m nacl. aliquots of this preparation were diluted with appropriate buffers to obtain samples in varying concentrations of nacl, and components of these samples were sedimented through sucrose solutions and dissolved in 0.01 m tris-hci buffer, ph 7.5. the protein content and sedimentation properties of components in these preparations were determined. when the nacl molarity in ...	1982	18635126
a neutron scattering study of the structure of compact and swollen forms of southern bean mosaic virus.	neutron small-angle scattering in h2o/d20 buffers has been used to investigate the radial distribution of protein and rna in the compact and swollen forms of southern bean mosaic virus (sbmv). the architecture of the particle is described by a set of four concentric shells. compact sbmv was found to consist of a tightly packed outer protein shell, with the rna and about 15% of the protein at the interior of the particle. the protein penetrates as deeply as the rna toward the center. this structu ...	1982	18635139
phosphorus-31 nuclear magnetic resonance study of internal motion in ribonucleic acid of southern bean mosaic virus.	phosphorus-31 nuclear magnetic resonance spectroscopy was used to study southern bean mosaic virus (sbmv) in its native and ethylenediaminetetraacetic acid treated forms at various ph values in the range 4.5-9.0. the degree of swelling in the latter form is a critical function of ph. spin-lattice relaxation time (t1), line-width, and nuclear overhauser effect (noe) measurements were made at 40.51, 80.98, and 190.3 mhz. the results indicate that the rna phosphate groups in native sbmv exhibit low ...	1982	6816273
detection of plant virus by using purified igg in elisa.	an optimized elisa system for the detection of soybean mosaic virus was developed. purified anti-soybean mosaic virus igg, a secondary ovalbumin coat, and a clearly defined optimal concentration of enzyme-conjugated anti-soybean mosaic virus igg are used. the test detects purified virus, or purified virus added to leaf and seed extracts, at a concentration of 2.5 ng/ml. various buffers significantly affect detection sensitivity.	1981	7021576
temperature-induced structural stabilization of the encapsidated rna of southern bean mosaic virus and its biologic significance.	virions of southern bean mosaic virus (sbmv) show two distinct sensitivity patterns upon heating. infectivity loss and capsid denaturation occur concurrently if virions are exposed at 50-55 degrees in 0.1 m sodium phosphate buffer, ph 7.5, or 0.1 m glycine-phosphate buffer, ph 9.0. contrastingly, virions are inactivated without any detectable capsid damage at 65-70 degrees in 0.1 m sodium phosphate buffer, ph 6.0, 0.1 m tris-hci buffer, ph 7.5, or 0.1 m glycine-naoh buffer, ph 9.0. heat treatmen ...	1981	18635030
chemical and serological properties of a cyanogen bromide peptide of southern bean mosaic virus protein.	southern bean mosaic virus (sbmv) protein was cleaved with cyanogen bromide and a highly basic peptide, cb-1, was isolated by ion exclusion and ion-exchange chromatography. twelve peptides were separated from a tryptic digest of cb-1 by ion-exchange chromatography and the composition of these peptides was similar to that of peptides released from edta-swollen virus particles by limited tryptic digestion. the composition and n-termini of the tryptic peptides indicated cb-1 was from the n-terminus ...	1980	7237270
ph-dependent urea sensitivity of southern bean mosaic virus.	the behavior of southern bean mosaic virus (sbmv) virions in urea was markedly ph dependent and revealed several novel features. in the absence of urea (5 degrees , 1 hr) sbmv was stable as a 115 s entity between ph 2.5 and 9.5, yielded a slowly sedimenting (ca 60-65 s) entity at ph 10.5, and was degraded into a 35-40 s product at ph 11.0. in 4.0 m urea, sbmv was precipitated irreversibly at ph 2.5 or 10.0, yielded variously sedimenting aggregates at ph 3.0, was transformed progressively into a ...	1979	18627892
replication of pea enation mosaic virus rna in isolated pea nuclei.	isolated nuclei from healthy pea plants were primed with pea enation mosaic virus (pemv), southern bean mosaic virus (sbmv), radish mosaic virus (rdmv), tobacco mosaic virus (tmv), pemv rna, sbmv rna, rdmv rna, or tmv rna. rna replication occurred only with pemv rna and not with intact pemv or any of the other viruses or rnas, as judged by ensuing actinomycin d-insensitive polymerase activity. molecular hybridization experiments showed that some of the product of the polymerase was pemv-specific ...	1977	16592421
differential effect of 2-thiouracil on synthesis of two plant viruses in the same host.	in cowpea leaves singly or doubly infected with cowpea chlorotic mottle virus (ccmv) and/or southern bean mosaic virus (sbmv), treatment with 2-thiouracil increased the accumulation of ccmv particles and strongly decreased the accumulation of sbmv particles. thiouracil prevented the usual inhibition of synthesis of ccmv particles at about 6 days after inoculation, and 2-3 times as many ccmv particles accumulated as in water-treated plants. a single treatment of thiouracil 48 h before, at the tim ...	1977	870449
possibilities for inter- and intracellular translocation of some icosahedral plant viruses.	southern bean mosaic virus (sbmv) and tomato ringspot virus (tomrv) were compared with regard to possible ways of inter- and intracellular translocation. the pore complexes in the nuclear membranes of nuclei in leaf palisade and mesophyll cells of several plant species commonly used in plant virus research were studied. the pore structure resembled that earlier described. the diameter of the pores was great enough to allow icosahedral plant viruses between 25 and 30 mmicro wide to move through. ...	1969	5765766
[intracellular inclusions of soybean mosaic virus].		1968	5753639