Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| construction of a chimaeric type 1/type 2 poliovirus by genetic recombination. | a chimaeric poliovirus carrying a type-2-specific neutralization epitope on a type 1 capsid was created by site-directed mutagenesis of the mahoney strain of poliovirus type 1. an ecorv and a hindiii restriction sites were first constructed in the cdna of poliovirus type 1 at nucleotide positions 2756 and 2786, respectively, i.e. on either side of the sequence encoding neutralization epitope c3 (vp1 amino acids 93-103), which is part of neutralization site nimi. the cdna sequence framed by the t ... | 1988 | 2849960 |
| a study of hpv 1, 2 and 4 antibody prevalence in patients presenting for treatment with cutaneous warts to general practitioners in n. ireland. | three hundred and seventy-six patients attending their general practitioner with cutaneous warts at five health centres in northern ireland were screened for human papilloma virus (hpv) types 1 and 2 igm antibody using an indirect immunofluorescence test. eight-eight (23.4%) patients were positive for hpv type 1 igm and 156 (41.5%) for hpv type 2 igm. hpv 1 igm antibody was significantly more likely to be associated with plantar warts than warts elsewhere (p less than 0.0001). hpv 2 igm was pres ... | 1988 | 2850937 |
| potential contribution of mild pepsin treatment at ph4 to the viral safety of human immunoglobulin products. | different manufacturers use several different processes for the production of intravenous immunoglobulin. several manufacturers include a production step where the immunoglobulin is treated with low levels of pepsin at ph 4. a series of experiments were undertaken to assess whether or not ph 4/pepsin treatment could inactivate a range of test viruses. acid-labile viruses such as vaccinia, herpes simplex, mumps and semliki forest virus were found to be susceptible to ph 4/pepsin treatment whereas ... | 1988 | 3142151 |
| engineering a poliovirus type 2 antigenic site on a type 1 capsid results in a chimaeric virus which is neurovirulent for mice. | poliovirus type 2 (pv-2) lansing strain produces a fatal paralytic disease in mice after intracerebral injection, whereas poliovirus type 1 (pv-1) mahoney strain causes disease only in primates. atomic models derived from the three-dimensional crystal structure of the pv-1 mahoney strain have been used to locate three antigenic sites on the surface of the virion. we report here the construction of type 1-type 2 chimaeric polioviruses in which antigenic site 1 from the pv-1 mahoney strain was sub ... | 1988 | 2460345 |
| vaccine-associated contact paralytic poliomyelitis with atypical neurological presentation. | paralytic poliomyelitis presenting with quadriparesis, transient encephalitis and bulbar symptoms in 2 patients in close contact with recently vaccinated children with trivalent live oral polio vaccine is described. symmetrical lower motor neuron involvement of deltoid muscles with electromyographic confirmation was found. upper motor neuron signs, with symmetrical hyperactive deep tendon reflexes developed in the lower extremities. poliovirus type-2 vaccine-like strain was cultured from one pat ... | 1987 | 3687370 |
| lansing poliovirus infection in mice: antibody demonstrable by enzyme-linked immunosorbent assay (elisa) and immunoprecipitation but not by neutralization. | adult mice infected intracerebrally (i.c) with the lansing strain of type 2 human poliovirus (hpv2) failed to develop a systemic neutralizing antibody response until 2 months post-infection (p.i). in contrast, an enzyme-linked immunosorbent assay (elisa) demonstrated an antibody response of igm and igg classes beginning at day 1 p.i. with peak levels reached by 5 weeks p.i. this response was slightly greater in paralyzed than in nonparalyzed animals. immunoprecipitation of poliovirus proteins fr ... | 1987 | 3027122 |
| comparison between indirect immunofluorescence and microneutralization for detection of antibodies to polioviruses. | indirect immunofluorescence and microneutralization methods for the detection of antibodies to poliovirus serotypes 1, 2, and 3 were compared. of the 41 sera tested for poliovirus type 1 antibody, 40% were in complete agreement, 55% differed by one dilution, and 5% differed by two dilutions. for poliovirus type 2, 37 sera were tested; 56% completely agreed, and 44% differed by only one dilution. for poliovirus type 3, complete agreement occurred in 59% of 33 sera, while 41% differed by one dilut ... | 1987 | 3038952 |
| identification of proteins encoded by the l1 and l2 open reading frames of human papillomavirus 1a. | the human papillomavirus 1 (hpv-1) virion is composed of two virally encoded proteins: a 57,000-molecular-weight polypeptide (57k polypeptide), which is the product of the l1 open reading frame (orf), and a 78k polypeptide, which is derived from the l2 orf. the 57k (l1) product, which represents the major structural component, appears to be disulfide cross-linked in virus particles. the 78k (l2) protein is a minor component of the virion and does not appear to be disulfide linked either to the l ... | 1987 | 3039170 |
| inhibition of poliovirus uncoating by disoxaril (win 51711). | disoxaril [win 51711, 5-[7-[4(4,5-dihydro-2-oxazolyl)phenoxy]heptyl]-3- methylisoxazole] inhibits the replication of polioviruses types 1 and 2 in hela cells by stabilizing the virus capsid, which results in the inhibition of the ph-dependent viral uncoating in endosomes and/or lysosomes. as shown by electron microscopy the virus entered into the cell by receptor-mediated endocytosis via coated pits and coated vesicles into endosomes irrespective of the presence or absence of the compound. measu ... | 1987 | 2820136 |
| the morphology of butchers' warts as related to papillomavirus types. | hand warts were studied in 160 butchers. clinical and histological studies were performed in 190 warts and virological studies in 165 warts from 104 butchers. since we found almost perfect correlation between the histological pattern and the type of infecting virus, it was possible to evaluate the virus types in a further 39 of 56 butchers without virological studies, on the basis of the histology of the warts. the most common infection was with hpv-2 (human papilloma virus) and hpv-7. thirty-th ... | 1987 | 2821930 |
| reduced mouse neurovirulence of poliovirus type 2 lansing antigenic variants selected with monoclonal antibodies. | the lansing strain of poliovirus type 2 is a mouse-adapted virus that induces a fatal paralytic disease in mice after intracerebral inoculation. our previous results indicated that the mouse-adapted phenotype maps to the lansing viral capsid. to further define regions of the capsid that are specifically involved in the infection of mice, antigenic variants resistant to neutralization with monoclonal antibodies were selected, and their mouse neurovirulence was studied. the monoclonal antibodies u ... | 1987 | 2825415 |
| in vitro antiviral activity of a saponin from anagallis arvensis, primulaceae, against herpes simplex virus and poliovirus. | the antiviral activity of a triterpene saponin isolated from anagallis arvensis, primulaceae, was studied in vitro against several viruses including herpes simplex type 1, adenovirus type 6, vaccinia, vesicular stomatitis and poliovirus. the drug was found to inhibit the replication of herpes simplex virus type 1 and poliovirus type 2 as shown by inhibition of cytopathic effect and reduction of virus production. the action was not due to a virucidal effect but might involve inhibition of virus-h ... | 1987 | 2825589 |
| intratypic differentiation of poliovirus strains by enzyme-linked immunosorbent assay (elisa): poliovirus type 2 and poliovirus type 3. | a double-antibody sandwich elisa was developed for the detection of antigenic differences between wild and vaccine-derived strains of poliovirus type 2 and poliovirus type 3. antibodies were prepared in rabbits by immunization with purified antigens of vaccine strains (type 2: sabin p712, and type 3: sabin leon) and wild type strains (type 2: mef, and type 3: pool 30). immunoblotting analysis of all antisera demonstrated that the igg antibodies raised in rabbits have specificity towards the main ... | 1987 | 2826518 |
| antigenic and biochemical characterization of poliovirus type 2 isolated from two cases of paralytic disease. | three isolates of poliovirus type 2 were obtained from rectal and pharyngeal swabs of two patients with paralytic poliomyelitis from southern italy (1981/1983). neither one of the patients had received oral poliovirus vaccine, although a systematic vaccination campaign is conducted in the area. neutralization tests with strain-specific antisera and analysis of the structural proteins by sds-page indicated that the three isolates were typical sabin-like poliovirus type 2. moreover, neutralization ... | 1987 | 2447031 |
| human papillomavirus type 2 dna in oral verrucous carcinoma. | tissues from patients with oral verrucous carcinoma were examined for the presence of human papillomavirus (hpv). the tissues were stained for the presence of the type common papillomavirus antigen by immunohistochemical staining and the presence of hpv dna was determined by in situ hybridization with biotin-labelled hpv dna probes. seventeen tissue specimens were obtained from 9 patients, and included pre-malignant lesions and primary and recurrent tumors. one pre-malignant lesion was positive ... | 1986 | 3027288 |
| mapping of sequences required for mouse neurovirulence of poliovirus type 2 lansing. | intracerebral inoculation of mice with poliovirus type 2 lansing induces a fatal paralysis, while most other poliovirus strains are unable to cause disease in the mouse. to determine the molecular basis for lansing virus neurovirulence, we determined the complete nucleotide sequence of the lansing viral genome from cloned cdna. the deduced amino acid sequence was compared with that of two mouse-avirulent strains. there are 83 amino acid differences between the lansing and sabin type 2 strain and ... | 1986 | 3003384 |
| identification of the human papilloma virus-1a e4 gene products. | antibodies prepared against a human papilloma virus-1 (hpv-1) e4/beta-galactosidase fusion protein identified several polypeptides in hpv-1, but not hpv-2 or 4, induced papillomas. the major e4 protein, that represented up to 30% of total cellular protein, was a 16/17-k doublet which was purified by column chromatography and analysed for amino acid content. a peptide derived by chymotryptic digestion was purified by h.p.l.c. and subjected to amino acid sequencing. the unique sequence obtained, g ... | 1986 | 3011404 |
| [papilloma viruses in benign and malignant tumors of the mouth and upper respiratory tract]. | a total of 113 benign and malignant tumours of the mouth and upper respiratory tract have been analysed for the presence of papilloma virus dna. thirty-four (63%) of 54 papillomatous lesions were found to contain such dna. seventy-two percent of the laryngeal papillomas contained hpv 6 or hpv 11 dna, whereas the oral papillomatoses harboured hpv 6 (15.6%), hpv 11 (9.4%), hpv 7 (9.4%), hpv 13 (12.5%) and hpv 32 (9.4%) dnas. the high number of hpv 7 dna-positive lesions was unexpected. in most of ... | 1986 | 3012230 |
| [bowenoid papulosis and its relation to bowen's disease and atypical condyloma]. | all the 5 sexual partners of male patients suffering from bowenoid papulosis and bearing the hpv 16 genome showed cervical lesions induced by hpv. 2 of them proved to be noncondylomatous infections, 3 cases were cervical condylomas, and 2 patients revealed severe dysplasia or carcinoma in situ (cis). 3 out of 4 women suffering from multicentrical pigmented bowen's disease of the vulva showed plain condylomas of the cervix, with severe dysplasia or cis in 2 cases and slight dysplasia in one case. ... | 1986 | 3012895 |
| papillomavirus dna in warts of immunosuppressed renal allograft recipients. | renal allograft recipients were shown to have an increased incidence of warts and skin cancers. we examined 148 patients for evidence of wart virus infections and tested for papillomavirus types, which are known to be associated with human malignancies. of the 148, 36 (24.3%) patients were afflicted with warts at the end of our study period, in contrast to 5 of 148 (3.3%) before transplantation. dna from 16 different biopsies was extracted by phenol treatment for further virological studies. dna ... | 1986 | 3015050 |
| prevention of rhinovirus and poliovirus uncoating by win 51711, a new antiviral drug. | win 51711, a potent new antipicornavirus drug, has been shown to inhibit an early event in the replication cycle of human poliovirus type 2 and human rhinovirus type 2. win 51711 was not virucidal and had no measurable effect on the adsorption of [3h]uridine-labeled virions to cells. when virion penetration of the plasma membrane was determined through loss of sensitivity to neutralizing antisera, win 51711 had no effect on poliovirus penetration, but inhibited rhinovirus penetration by 40%. in ... | 1986 | 3019232 |
| ultrastructural immunohistochemical localization of poliovirus during virulent infection of mice. | ultrastructural immunohistochemistry was used to localize type 2 human poliovirus (hpv 2) during virulent infection of mice caused by the lansing strain. in the spinal cord, immune-reaction product was exclusively localized within neurons and their processes. the absence of viral antigen in glial, endothelial and inflammatory cells further supports the strict neuronotropicity of hpv. in addition, viral antigen and virus-like particles were localized in synaptic complexes and axons, including pre ... | 1986 | 3021913 |
| human papillomavirus infections in a group of renal transplant recipients. | one hundred and twenty renal transplant recipients were investigated. fifty-eight (48%) were found to have warts, 13 (11%) keratoses and six (5%) to have, or recently to have had cancers. the longer the time of immunosuppression, the greater the prevalence of warts; of those patients who had had their transplant for at least 5 years, 87% had warts. those with a graft survival time of 10 years or more are at special risk of warts, keratoses and malignancy. five (10%) of 50 women had genital warts ... | 1986 | 3026431 |
| [urogenital and anal papillomavirus infections]. | recently virologists and clinicians have focused attention on infections with human papillomaviruses (hpv). this is due to the ubiquity, the increasing frequency and the possible association of these viruses with the development of squamous cell carcinomas of the skin and of the mucous membranes of the respiratory, gastrointestinal, genitourinary and anorectal tracts. hpv represent a very heterogeneous group of dna tumor viruses. by means of molecular-biological techniques, more than 40 hpv type ... | 1986 | 3026998 |
| oral efficacy of win 51711 in mice infected with human poliovirus. | win 51711, a new broad-spectrum anti-picornavirus agent, prevented the development of paralysis and subsequent death in mice infected intracerebrally with a lethal dose of human poliovirus type 2 (mef strain). the prophylactic efficacy of intragastrically administered win 51711 was dose dependent over the 3.9- to 62.5-mg/kg (twice daily) dose range, with a minimal significantly effective dose of less than 15.6 mg/kg per dose (twice daily) (p less than 0.008). an oral four times a day dosage regi ... | 1986 | 3729333 |
| fatal disseminated infection due to poliovirus type 2 vaccine. | 1986 | 3776928 | |
| [poliovirus type 2 in sewage canals in prague in 1981-1982]. | 1985 | 2985289 | |
| isoxazoles with antipicornavirus activity. | the synthesis and evaluation of a series of 3,5-disubstituted isoxazoles as antipicornavirus agents have led to the discovery of several compounds effective in vitro against rhinovirus type 2 and poliovirus type 2. compound 32 was found more effective than 4',6-dichloroflavan against both viruses and was evaluated orally in mice infected intracerebrally with polio-2. at 31 mg/kg bid, compound 32 showed a 53% survival rate as compared to 22% for the nonmedicated animals. | 1985 | 2989517 |
| papillomavirus dna in human tongue carcinomas. | seven biopsies from different carcinomas of the tongue were analyzed for human papilloma virus (hpv) sequences by southern blot analysis. after hybridization with various types of human papilloma viruses, 3 tumors were found to be positive. whereas dna from one tumor hybridized with hpv 2 dna under conditions of high stringency, the 2 other positive biopsies contained hpv 16 dna. all positive tumors revealed high copy numbers of the respective dna. the cleavage pattern of the hpv-2-positive tumo ... | 1985 | 2997044 |
| poliovirus and echovirus survival in tetrahymena pyriformis culture in vivo. | axenic cultures of tetrahymena pyriformis, strain i mt iv, grown in a defined medium at room temperature, were used to study interactions of these protozoa with vaccination strain l sc 2ab of poliovirus type 1, vaccination strain p 712 of poliovirus type 2 and with type 30 echovirus, strain 480/78. t. pyriformis cultures in media containing 10(3.0) tcd50/1 ml of type poliovirus, 10(3.0) tcd50/1 ml of type 2 poliovirus or 10(2.5) tcd50/1 ml echovirus 30 and in virus-free medium did not differ one ... | 1984 | 6088623 |
| stability of hepatitis a virus. | the stabilities of hepatitis a virus (hav) and of poliovirus type 2 were compared under strictly controlled, identical conditions of ph value, temperature, and salt concentration. although the resistance of the viruses proved to be the same from ph 3 to 11, the temperature at which 50% of poliovirus particles became disintegrated during heating at ph 7.0 for 10 min (t50,10 = 43 degrees) differed significantly from that characteristic for hav (t50,10 = 61 degrees). in the presence of 1 m mgcl2, t ... | 1984 | 6096294 |
| serum antibodies to poliomyelitis in italy. | a seroepidemiological survey was conducted to assess the effectiveness of the italian nationwide immunization programme against poliomyelitis. over 3800 serum samples were collected from persons, aged between 6 months and 79 years and residing in urban and rural areas with different socioeconomic conditions and geographic locations.a very high level of protection was found in the target population, confirming the almost 100% history of complete immunization. almost all subjects over 30 years of ... | 1984 | 6333296 |
| long-term immunity following vaccination with killed poliovirus vaccine in sweden, a country with no circulating poliovirus. | the long-term immunity following vaccination with killed poliovirus vaccine was analyzed by making repeated antibody surveys. a comparison between the levels of antibody of vaccinated groups of children and young adults in 1968 and 1978 indicated that mean levels of antibody of the age groups that, during the period 1968-1978, had not received any extra booster differed by less than or equal to 0.3 log10. children born in the period 1964-1967 and young adults born in the period 1960-1963 were co ... | 1984 | 6740100 |
| characterization of human parainfluenza viruses. i. the structural proteins of parainfluenza virus 2 and their synthesis in infected cells. | two strains of human parainfluenza virus 2 (hpv2), p2 1972/6 and p2 1980, grow to high titre in mek3 cells, and their structural proteins and virus-induced protein synthesis have been characterized by gel electrophoresis and immunoprecipitation. purified viruses contain seven polypeptides, including cellular actin: l (175k mol. wt.), hn (72k to 74k), np (66k to 67k), f1 (52k to 58k), p (49k), a (44.5k) and m (39k). virus-induced polypeptide synthesis was first detected at 8 h post-infection with ... | 1983 | 6311949 |
| human papillomaviruses associated with epidermodysplasia verruciformis. ii. molecular cloning and biochemical characterization of human papillomavirus 3a, 8, 10, and 12 genomes. | the dnas of four human papillomaviruses (hpvs) that were found in the benign lesions of three patients suffering from epidermodysplasia verruciformis have been characterized. the flat wart-like lesions and the macular lesions of patient 1 contained two viruses, hpv-3a and hpv-8, respectively, whose genomes had previously been only partially characterized. the flat wart-like lesions of patient 2 and the macular lesions of patient 3 each contained a virus previously considered as belonging to type ... | 1983 | 6312091 |
| identification of human papillomavirus dna in cervical and vaginal intraepithelial neoplasia with molecularly cloned virus-specific dna probes. | the presence of human papillomavirus (hpv) dna was identified in the tissues of cervical and vaginal intraepithelial neoplasia by southern blot dna hybridization under conditions of low stringency. the specific types of hpv present in the tissues were identified by using molecularly cloned types 1 through 6 (hpv-1 through hpv-6) hpv dna probes under high-stringency conditions. all tissues of cervical and vaginal intraepithelial neoplasia analyzed contained hpv genomes. fifteen of 19 samples (79% ... | 1983 | 6313534 |
| inactivation of hepatitis a virus and indicator organisms in water by free chlorine residuals. | hepatitis a virus (hav) and selected indicator organisms were mixed together in chlorine-demand-free buffers at ph 6, 8, or 10 and exposed to free chlorine residuals, and the survival kinetics of individual organisms were compared. hav was enumerated by a most-probable-number dilution assay, using plc/prf/5 liver cells for propagation of the virus and radioimmunoassay for its detection. at all ph levels, hav was more sensitive than mycobacterium fortuitum, coliphage v1 (representing a type of ph ... | 1983 | 6314894 |
| production of hybrid cell lines secreting monoclonal antibodies against poliovirus type 2. | 1983 | 6317298 | |
| introduction of cloned human papillomavirus genomes into mouse cells and expression at the rna level. | the entire dna genomes of five different human papillomaviruses (hpvs) were cloned into the bamhi site of pbr322 (hpv-1a, hpv-3, hpv-4, and hpv-9) or the ecori site of pbr325 (hpv-2), using as starting materials virus preparations isolated from papillomas of individual patients. under stringent hybridization conditions (tm-28 degrees), the five cloned hpvs exhibited less than 10% homology with one another. to establish model cell systems that may be useful for the identification of hpv genes and ... | 1983 | 6193636 |
| effect of oral aromatic retinoid (ro 10-9359) on human papilloma virus-2-induced common warts. | persisting human papilloma virus (hpv) 2 induced common warts of a chronic lymphatic leukemia patient were orally treated with aromatic retinoid ro 10-9359 (tigason). clinically, the lesions improved rapidly. virus-specific cytopathogenic effects (cpe), virus particles and viral dna were no longer detectable. therapy was discontinued because of the development of a liposarcoma, which led to a complete relapse of the cutaneous lesions. hpv-2 specific parameters, cpe and viral dna, were restored. ... | 1983 | 6840376 |
| prevention of human poliovirus-induced paralysis and death in mice by the novel antiviral agent arildone. | arildone, a novel antiviral agent which blocks virion uncoating, was assessed for its ability to prevent paralysis and death in mice infected intracerebrally with a lethal dose of human poliovirus type-2 (strain mef). intraperitoneal administration of arildone suspended in gum tragacanth prevented paralysis and death in a dose-dependent manner (minimal inhibitory dose = 32 mg/kg, twice daily) and protected animals from virus challenges in excess of 20 50% lethal doses. oral medication with arild ... | 1982 | 7159068 |
| antiviral activity and mechanism of action of 2-(3,4-dichlorophenoxy)-5-nitrobenzonitrile (mdl-860). | a nitrobenzene derivative, mdl-860, was found to inhibit plaque formation, cytopathic effect, or both in 11 of 12 picornaviruses at concentrations which did not affect cell growth. the compound did not directly inactivate the virus. mdl-860 inhibited actinomycin d-resistant [3h]uridine uptake in cells infected with coxsackievirus a21 or rhinovirus 1-a, whereas incorporation into uninfected cells was not inhibited. with three picornaviruses (echovirus type 12, poliovirus type 2, and rhinovirus ty ... | 1982 | 7181474 |
| correlation between human papillomavirus (hpv) type and histology of warts. | forty warts from different patients and of different clinical type were examined histologically and virologically. eight lesions were found to be associated with human papillomavirus type 1 (hpv 1), 15 tumors were induced by hpv 2, hpv 3 was detected 4 times, hpv 4 twice, and hpv 6 eleven times. hpv 3, hpv 4, and hpv 6 induced warts revealed a correlation between histology and virus type. they are characterized by the so called "edematous type clear cells". in hpv 3 associated flat warts pycnoti ... | 1982 | 6276473 |
| viral receptors on isolated murine and human ependymal cells. | viruses that infect ependyma cause ependymitis in humans and hydrocephalus in experimental animals. we report that reovirus type 1 (which induces hydrocephalus in mice) binds to the surface of isolated human and murine ciliated ependymal cells. with the use of recombinant viral clones, the binding property was mapped to the type 1 viral hemagglutinin, which also determines in vivo the affinity of reovirus type 1 for ependyma. mumps virus, measles virus, parainfluenza type 3, and herpes simplex v ... | 1982 | 6276976 |
| excretion of attenuated polioviruses in children vaccinated with live oral poliovirus vaccine. | the excretion of attenuated polioviruses was studied in a group of nursery children vaccinated with 105tcd50 of each type of virus. the primovaccinated children were found to excrete type 1 poliovirus for 8 weeks, type 2 for 11 weeks after the vaccination with the type 1 + 2 bivaccine. poliovirus type 1 as eliminated by 78% and type 2 by 98% of the vaccinees. the separately administered type 3 was detectable for 6 weeks and was isolated from 100% of the vaccinees. the highest per cent of childre ... | 1982 | 6282962 |
| alteration in oligonucleotide fingerprint patterns of the viral genome in poliovirus type 2 isolated from paralytic patients. | a close relationship was demonstrated by oligonucleotide fingerprinting between genomes of the poliovirus type 2 sabin vaccine strain and recent isolates from paralytic cases associated with vaccination in japan. the oligonucleotide maps of isolates from an agammaglobulinemic patient, who continued to excrete poliovirus type 2 for 3.5 years after the administration of oral vaccine, showed that the genomic alteration proceeded gradually, retaining the majority of the oligonucleotides characterist ... | 1982 | 6179881 |
| the physicochemical properties of infectious hepatitis a virions. | the propagation of hepatitis a virus (hav) in the cell line plc/prf/5 made possible the radiolabelling in vivo of mature, infectious hepatitis a virions and the determination of their physicochemical properties. in contrast to poliovirus type 2 (160s, 1.340 g/ml), hav had a sedimentation coefficient of 156 +/- 2s and a buoyant density of 1.332 g/ml in cscl. the genome of hav consisted of linear single-stranded rna which sedimented at 32.5s under non-denaturing conditions. compared to the size an ... | 1981 | 6172556 |
| restriction map of poliovirus type 2 cdna. | poliovirus type 1 rna was reverse-transcribed into c-dna and inserted at the pst i site of the plasmid vector pbr322 of e. coli. resulting recombinant plasmids were analyzed by hybridization with rnase t1-resistant 32p-labeled oligonucleotides, and by restriction enzyme mapping. all of the genome was cloned in a series overlapping cdna inserts, the longest of which was 3.2 kb. the restriction map of the poliovirus cdna is presented. | 1981 | 6176488 |
| [dual infection of mycobacterium tuberculosis and poliovirus type 2 (author's transl)]. | 1981 | 6801154 | |
| identification of papillomaviruses in butchers' warts. | we have studied the papillomaviruses found in the hand warts of 60 butchers, most of them from 2 distant slaughterhouses. warts differing in morphology and location were studied separately. the viruses were identified by molecular hybridization, restriction enzyme analysis and immunofluorescence. four known human papillomaviruses (hpv-1, hpv-2, hpv-3, hpv-4) were detected and one hitherto unknown papillomavirus was identified in 9 butchers. the dna of the latter virus did not anneal with any of ... | 1981 | 6257792 |
| giant cell pneumonia associated with parainfluenza virus type 3 infection. | giant cell pneumonia associated with parainfluenza virus type 3 infection and chronic poliovirus type 2 meningoencephalomyelitis are documented in an infant with combined immunologic deficiency (swiss type). caution should be exercised in attributing cases of giant cell pneumonia to measles virus without serologic or virologic evidence. | 1981 | 6265340 |
| structural proteins of poliovirus type 2 isolates. | seventeen strains of type 2 poliovirus isolated from vaccines and vaccine-contacts in japan were analyzed for viral structural proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis. an alteration in electrophoretic mobility of vp1 was observed in most of the strains from paralytic patients and healthy vaccinees. the alteration in vp1 was observed at a high frequency among many strains, but no significant relation between the mobility change of vp1 and antigenic marker or rct/40 m ... | 1981 | 6268945 |
| inactivation of poliovirus by chloramine-t. | since concern has recently been expressed about the presence of genotoxic substances due to chlorination of water and wastewater, chloramine-t (cat) is proposed as an alternative disinfectant to chlorine. the viricidal properties of chlorine and cat were compared. kinetics of inactivation of poliovirus type 2 by chlorine and cat in chlorine demand-free water were investigated by using a kinetic apparatus. inactivation of the virus by chlorine and cat occurred in two steps. the initial linear par ... | 1981 | 6271058 |
| poliovirus adsorption by 34 minerals and soils. | the adsorption of radiolabeled infectious poliovirus type 2 by 34 well-defined soils and mineral substrates was analyzed in a synthetic freshwater medium containing 1 mm cacl(2) and 1.25 mm nahco(3) at ph 7. in a model system, adsorption of poliovirus by ottawa sand was rapid and reached equilibrium within 1 h at 4 degrees c. near saturation, the adsorption could be described by the langmuir equation; the apparent surface saturation was 2.5 x 10(6) plaque-forming units of poliovirus per mg of ot ... | 1981 | 6274259 |
| influence of ph and electrolyte composition on adsorption of poliovirus by soils and minerals. | the ph and the nature an concentration of simple electrolytes influenced the interaction of poliovirus type 2 with three soils, a sand, and a clay mineral. in electrolytes above ph 9 the virus was not adsorbed extensively to the substrates, but below ph 7 almost all virus was bound. for each adsorbent there was a characteristic ph region of transition from strong to weak uptake. differences between the soils in virus uptake were shown to parallel their ph-dependent mineral. in electrolytes above ... | 1981 | 6274260 |
| sequence of picornavirus rnas containing a radioiodinated 5'-linked peptide reveals a conserved 5' sequence. | virion rna (vrna) from poliovirus type 1 (pv1), poliovirus type 2 (pv2), and coxsackie virus b1 (cox b1) were treated with proteinase k to remove all but a small peptide of the covalently attached 5' genome-linked virion protein (vpg). the peptide on these rna molecules was then treated with bolton-hunter 125i reagent, which iodinates primary amine groups, in order to obtain specific 5'-terminal radioactive labeling. sequences of 125i-labeled vrnas were determined by using a set of base-specific ... | 1980 | 6244553 |
| the genome-linked protein of picornaviruses. vi. the 5'-terminal protein of poliovirus type 2rna is covalently linked to a nonanucleotide identical to that of poliovirus type 1 rna. | digestion of poliovirus type 2[32p]-rna with enzymes and analysis of the products by column chromatography and paper electrophoresis provides evidence that the rna is covalently linked to a small, basic protein, vpg, via a 5'-terminal pu residue. digestion of the rna with proteinase k followed by labeling of the peptidyl-rna with the bolton and hunter reagent [iodinated 3-(4-hydroxyphenyl)propionic acid n-hydroxysuccinimide ester] yields 5'-labeled material suitable for rapid sequencing. the 5'- ... | 1980 | 6246035 |
| relationships between environmental factors, bacterial indicators, and the occurrence of enteric viruses in estuarine sediments. | current standards for evaluation of the public health safety of recreational and shellfish-harvesting waters are based upon bacteriological analysis, but do not include an evaluation of the number of viruses. the objective of this study was to determine the occurrence of enteric viruses in estuarine sediments and to find a relationship, if any, between the presence of viruses in seawater or sediment or both and various biological and physicochemical characteristics of the environment. viruses we ... | 1980 | 6247974 |
| anogenital warts contain several distinct species of human papillomavirus. | anogenital warts from 26 patients were examined for the presence of human papillomavirus (hpv). although no whole, intact virus could be identified, varying amounts of nonintegrated hpv dna were detected in 18 tissue specimens (70%) by employing both an agarose gel-ethidium bromide staining method and the southern blot hybridization procedure. when hybridization analysis was performed under stringent conditions, six anogenital warts were observed to contain hpv genomic sequences related to eithe ... | 1980 | 6255208 |
| vaccine-associated poliomyelitis in an infant with agammaglobulinemia. | we describe a female infant with agammaglobulinemia who contracted vaccine-associated poliomyelitis. poliovirus type 2 was isolated from the initial stool specimen. in our patient, temporary changes in the cerebrospinal fluid resembled those in patients without immunodeficiencies, although gammaglobulin therapy had not yet been started. pleocytosis was observed for a short time after viremia, but soon there was a return to normal without antibody production. | 1980 | 6255734 |
| assay of human interferon in vero cells by several methods. | four methods for the assay of human interferon in vero cells were compared based on the inhibition of viral cytopathic effect (cpe) in tubes, the inhibition of cpe in microplates, the reduction of plaques, and the inhibition of quantitative hemadsorption. for inhibition of cpe, sindbis virus, vesicular stomatitis virus, poliovirus type 2, and vaccinia virus were used for challenge. in the plaque reduction method, sindbis virus, vesicular stomatitis virus, and poliovirus were employed, and newcas ... | 1979 | 222803 |
| sequence studies of poliovirus rna. iv. nucleotide sequence complexities of poliovirus type 1, type 2 and two type 1 defective interfering particles rnas, and fingerprint of the poliovirus type 3 genome. | the 32p-labelled genomes of poliovirus type 1, 2 and 3 have been digested with rnase t1 and the products separated by two-dimensional gel electrophoresis. all three fingerprints differ in the separation pattern of the large oligonucleotides. the molar yields of the large rnase t1-resistant oligonucleotides of type 1 and type 2 rna of poliovirus rna are close to one. by comparing the yields of these oligonucleotides to the amount of rna from which they originated, the chain length of type 1 polio ... | 1979 | 230285 |
| paralytic poliomyelitis in a child with agammaglobulinemia. | this paper gives the clinical, immunological and virological data on a patient with agammaglobulinemia who developed paralytic poliomyelitis. the patient was a 3 year-old boy who had a typical b-cell defect without a t-cell defect. he had profound hypogammaglobulinemia and defective plasma cells and had repeated pyogenic infections which were controlled by gammaglobulin replacement therapy. at 3 years of age, he was admitted to our hospital with suspected meningitis. he had fewer, tremor and nec ... | 1979 | 315313 |
| poliomyelitis survey in middle-east countries. | the poliomyelitis survey was started in 1976 in bahrain and kuwait. for each paralytic case, diagnosis was done by virus isolation, neutralizing antibody titration and specific igm characterization. more than 90% of the infants who were ill were less than 2 years old. in bahrain, 17 paralytic cases in 1976 and 12 in 1977 occurred between january and july (annual incidence was respectively 7.7 and 5.4/100,000). the first epidemic was due to poliovirus type 2, the second one to poliovirus type1. f ... | 1978 | 223907 |
| characterization and classification of virus particles associated with hepatitis a. i. size, density, and sedimentation. | virus-like particles were purified from stools of patients in an epidemic of hepatitis a in germany. when reference ms-1 chimpanzee pre-inoculation and convalescent sera were used, the close serological relationship of the purified particles to well-known isolates of hepatitis a could be established. on the other hand, the physicochemical characteristics of the particles were determined in parallel to the characteristics of a marker parvovirus (luiii) and a marker picornavirus (poliovirus type 2 ... | 1978 | 206730 |
| characterization and classification of virus particles associated with hepatitis a. ii. type and configuration of nucleic acid. | virus particles banding at 1.34 g/ml in cscl and sedimenting at 160s in sucrose gradients were isolated from fecal specimens of patients suffering from hepatitis. in the presence of 4 m urea and about 90% formamide, these particles released linear nucleic acid molecules of the kinked appearance characteristic of single-stranded rna or single-stranded dna. they could be distinguished from the nucleic acid of phage lambda added to the preparation as a marker for double-stranded configuration. expe ... | 1978 | 206731 |
| interaction of liposomes with subviral particles of poliovirus type 2 and rhinovirus type 2. | subviral particles ("a particles") were produced from rhinovirus type 2 by treatment with acid and from poliovirus type 2 by incubation at 37 degrees c in a low-ionic-strength buffer. a particles, but not virions, adsorbed to liposomes. it is proposed that these reactions may provide an in vitro model for two early steps of infection. | 1976 | 183023 |
| polyadenylate sequences of human rhinovirus and poliovirus rna and cordycepin sensitivity of virus replication. | the polyadenylate [poly(a)] content of the genome rna of human rhinovirus type 14 (hrv-14) is nearly twice as large as that of the genome rna of poliovirus type 2. the poly(a) content of viral rna was determined to be the rnase-resistant fraction of 32p-labeled viral rna extracted from purified virions. polyacrylamide gel electrophoresis indicated that the poly(a) sequences of hrv-14 are more heterogenous and on an average larger than those of poliovirus rna. on the basis of susceptibility to mi ... | 1976 | 185411 |
| interaction of listeria monocytogenes and influenza in an animal model. | this study was designed to investigate the effects of viruses in the pathogenesis of listeria monocytogenes. the organisms used in this study were: listeria monocytogenes type 1 isolated from a local fatal case; mouse adapted influenza a/pr8/34 (honi); streptococcus pneumoniae group b (u.m. med. ctr.) and poliovirus type 2 mef--g3m2. balb-c mice were inoculated intraperitoneally with one ld50 of listeria monocytogenes. ten days later, the survivors were challenged intransally with 10 ld50 of inf ... | 1976 | 10263 |
| early alteration of poliovirus in infected cells and its specific inhibition. | hela cells infected with radioactive poliovirus type 2 were disrupted with ultrasonic treatment, followed by addition of a non-ionic detergent. two types of virus particles were found to sediment at 80 to 90% the rate of native virus. the first of these appeared to be a complex of native virus particles and membrane components, since treatment with 0-2% sds released infectious native particles. the second was non-infectious and its sedimentation rate was not greatly altered by sds. one hour afte ... | 1975 | 167116 |
| the effects of concanavalin a on the early events of infection by rhinovirus type 2 and poliovirus type 2. | the effect of concanavalin a (con a) on the course of early infection of hela cells with purified radioactive human rhinovirus type 2 (hrv-2) or poliovirus type 2 (p-2) has been examined. several early steps in infection were inhibited before the uncoating of parental virus. con a, at 100 mug/ml, reduces attachment of virus when added to cells before infection. con a also detectably slows the normal progression of adsorbed virus to tightly bound forms characterized, in the case of hrv-2, by resi ... | 1975 | 170375 |
| [effect of sodium salicylate on the "in vitro" development of rna viruses (author's transl)]. | sodium salicylate 10(-3) m is able to limit the number of plaques induced by rna viruses on monkey kidney cell cultures (cercopithecus aethiops and macaccus rhesus). however, the inhibition rate changes with the virus type or even the strain used. poliovirus 2, coxsackie b3, echovirus and reovirus 1 were the most susceptible to the inhibitory effect of the drug. latent infection caused by sv5 decreases the antiviral activity of salicylate. the stages of the poliovirus type 2 growth affected by s ... | 1975 | 171988 |
| [adsorption and elution of poliovirus type 2 strains in al (oh)3 gel]. | 1974 | 4376884 | |
| cleavage of poliovirus-specific polypeptide aggregates. | zonal electrophoresis resolves two aggregates of poliovirus type 2 cytoplasmic polypeptides. the more negatively charged aggregate contains mainly noncapsid viral-specific polypeptides (ncvp) 2 and x, whereas the other consists of the capsid polypeptides (vp) 0, 1, 2, and 3 (vp0, vp1, vp2, vp3). after treatment with sodium deoxycholate (doc), the aggregates sediment at 5 to 6s. their electrophoretic mobilities are unaffected by doc or rnase. the capsid polypeptide aggregate is similar in mobilit ... | 1973 | 4355854 |
| antiviral activity of tobacco smoke condensate on encephalomyocarditis infection in mice. | a water-soluble nontumorigenic acidic fraction of tobacco smoke condensate of cigarettes has been found to have antiviral activity against encephalomyocarditis (emc) virus infection in mice. the portion of lower molecular weight was inhibitory to the growth of emc virus, vesicular stomatitis virus, reovirus type 2, vaccinia virus, and poliovirus type 2, but not against adenovirus type 12, in kb cell cultures. the cigarette smoke agent did not induce serum interferon although it protected mice fr ... | 1973 | 4364177 |
| 3-deazauridine: inhibition of ribonucleic acid virus-induced cytopathogenic effects in vitro. | 3-deazauridine, a synthetic analogue of uridine and a potential antitumor agent, was found to possess antiviral activity against a number of ribonucleic acid-containing animal viruses in mammalian cell culture. inhibition of virus-induced cytopathogenic effects by 3-deazauridine was observed in cells infected with rhinovirus types 1a and 13, coxsackievirus type a21, and pr-8 influenza virus, but not in cells infected with poliovirus type 2 or echovirus type 12. the extracellular yield of progeny ... | 1972 | 4799550 |
| comparative studies on the mode of neutralization reaction of poliovirus type 2 with serum igg and secretory iga from mother's milk and fecal extract. | 1971 | 4328202 | |
| methods for detecting food-borne enteroviruses. | a method previously reported for detecting virus in a model system composed of cottage cheese contaminated with coxsackievirus type a9 has been adapted to detecting selected strains of enteroviruses in a variety of foods. bentonite is omitted and serum is added for extracting virus from low-protein foods. samples of foods, usually 25 g, must contain at least 3 to 4 plaque-forming units for a 50% probability of detecting virus. sensitivity in detecting echovirus type 6 was lower than that for the ... | 1968 | 4300896 |
| [cytomorphology and enzymocytochemistry in viral infections in tissue cultures. action of poliovirus, type 2]. | 1967 | 4168735 | |
| counter-current distribution of poliovirus type 2 and 3. | 1966 | 4289063 | |
| relationship between structure of benzimidazole derivatives and selective virus inhibitory activity. inhibition of poliovirus multiplication and cytopathic effects by 2-(alpha-hydroxybenzyl)-benzimidazole, and its 5-chloroderivative. | the virus inhibitory activity and selectivity of certain benzimidazole, benzotriazole, and naphthimidazole derivatives were determined with influenza b and polio type 2 viruses. among the sixty-five compounds examined, several were highly active inhibitors of influenza b virus multiplication in the chorioallantoic membrane in vitro. the following compounds, listed in order of increasing inhibitory activity, were more than 100 times as active as benzimidazole: 5-(4'-toluenesulfonamido)-benzimidaz ... | 1961 | 13775109 |
| presence of two interrferring enterovirral agents (echo virus type 9 and poliovirus type 2) in the human central nervous system. | 1961 | 13780920 | |
| differentiation of closely related cells by a variant of poliovirus, type 2, mef1 strain. | by application of a variant of poliovirus, type 2, mef(1) strain, as a selective agent it was possible to distinguish among stable parent strains of epithelial cells and their clonal derivatives by their differential morphologic response to infection. the variant of poliovirus grew in a number of cell strains without induction of observable cytopathogenic changes. other strains of cells reacted to viral infection by manifesting partial or complete degeneration. parent hela cells and virus underw ... | 1959 | 14425416 |