Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| feline leukemia virus and feline immunodeficiency virus article - a comment. | 2011 | 22547834 | |
| infectious disease prevalence in a feral cat population on prince edward island, canada. | ninety-six feral cats from prince edward island were used to determine the prevalence of selected infectious agents. the prevalence rates were 5.2% for feline immunodeficiency virus, 3.1% for feline leukemia virus, 3.1% for mycoplasma haemofelis, 8.4% for candidatus mycoplasma haemominutum, 2.1% for bartonella spp. and 29.8% for exposure to toxoplasma gondii. oocysts of t. gondii were detected in 1.3% of the fecal samples that were collected. gender and retroviral status of the cats were signifi ... | 2011 | 22379197 |
| feline leukemia virus and feline immunodeficiency virus in canada: recommendations for testing and management. | feline leukemia virus (felv) and feline immunodeficiency virus (fiv) are common and important infectious disease agents of cats in canada. seroprevalence data for felv and fiv in various populations of canadian cats are reviewed and recommendations for testing and management of infections by these viruses in cats in canada are presented. retrovirus testing in canada is infrequent in comparison with the united states, and efforts should be focused on reducing physical and other barriers to testin ... | 2011 | 22294790 |
| uterine adenocarcinoma with feline leukemia virus infection. | feline endometrial adenocarcinomas are uncommon malignant neoplasms that have been poorly characterized to date. in this study, we describe a uterine adenocarcinoma in a persian cat with feline leukemia virus infection. at the time of presentation, the cat, a female persian chinchilla, was 2 years old. the cat underwent surgical ovariohystectomy. a cross-section of the uterine wall revealed a thickened uterine horn. the cat tested positive for feline leukemia virus as detected by polymerase chai ... | 2011 | 22232645 |
| an all-feline retroviral packaging system for transduction of human cells. | abstract the subgroup c feline leukemia virus (felv-c) receptor flvcr is a widely expressed 12-transmembrane domain transporter that exports cytoplasmic heme and is a promising target for retrovirus-mediated gene delivery. previous studies demonstrated that felv-c pseudotype vectors were more efficient at targeting human hematopoietic stem cells than those pseudotyped with gibbon ape leukemia virus (galv), and thus we developed an all felv-c-based packaging system, termed catpac. catpac is helpe ... | 2010 | 20222826 |
| prevalence of hemotropic mycoplasmas in healthy and unhealthy cats and dogs in spain. | the aims of the present study were to determine the prevalence of hemoplasmas in cats and dogs from the barcelona area of spain with the use of species-specific quantitative polymerase chain reaction (qpcr) assays and to evaluate any associations between hemoplasma infection, clinical presentation, and vector-borne infections. blood samples from cats (191) and dogs (182) were included and were classified as healthy (149) or unhealthy (224). ethylenediamine tetra-acetic acid blood samples underwe ... | 2010 | 20224091 |
| feline leukemia virus integrase and capsid packaging functions do not change the insertion profile of standard moloney retroviral vectors. | adverse events linked to perturbations of cellular genes by vector insertion reported in gene therapy trials and animal models have prompted attempts to better understand the mechanisms directing viral vector integration. the integration profiles of vectors based on mlv, aslv, siv and hiv have all been shown to be non-random, and novel vectors with a safer integration pattern have been sought. recently, we developed a producer cell line called catpac that packages standard momlv vectors with fel ... | 2010 | 20237508 |
| feline immunodeficiency virus, feline leukemia virus and toxoplasma gondii in stray and household cats in kerman-iran: seroprevalence and correlation with clinical and laboratory findings. | this study was carried out to determine the seroprevalence of feline leukemia virus (felv), feline immunodeficiency virus (fiv) and toxoplasma gondii (t. gondii) infection among stray and owned cats in southeastern iran and to identify the influence of age, sex, lifestyle, health status, and laboratory findings on seropositivity. the overall infection rate for fiv, felv, and t. gondii was 19.2%, 14.2%, and 32.1% respectively. results of the multivariate logistic regression analysis showed that o ... | 2010 | 20362315 |
| transduction of human primitive repopulating hematopoietic cells with lentiviral vectors pseudotyped with various envelope proteins. | lentiviral vectors are useful for transducing primitive hematopoietic cells. we examined four envelope proteins for their ability to mediate lentiviral transduction of mobilized human cd34(+) peripheral blood cells. lentiviral particles encoding green fluorescent protein (gfp) were pseudotyped with the vesicular stomatitis virus envelope glycoprotein (vsv-g), the amphotropic (ampho) murine leukemia virus envelope protein, the endogenous feline leukemia viral envelope protein or the feline leukem ... | 2010 | 20372106 |
| high prevalence of non-productive felv infection in necropsied cats and significant association with pathological findings. | applying a combination of semi-nested pcr and immunohistology (ihc), the presence of exogenous feline leukemia virus infection was studied in 302 necropsied cats with various disorders. 9% showed the classical outcome of persistent productive felv infection which was represented by felv antigen expression in different organs. 152 cats (50%) harboured exogenous felv-specific proviral sequences in the bone marrow but did not express viral antigen. these cats were considered as horizontally but non ... | 2010 | 20398945 |
| encephalomyelitis resembling human and ruminant rhombencephalitis caused by listeria monocytogenes in a feline leukemia virus-infected cat. | 2010 | 20412434 | |
| the frequency of occurrence and nature of recombinant feline leukemia viruses in the induction of multicentric lymphoma by infection of the domestic cat with felv-945. | during feline leukemia virus (felv) infection in the domestic cat, viruses with a novel envelope gene arise by recombination between endogenous felv-related elements and the exogenous infecting species. these recombinant viruses (felv-b) are of uncertain disease association, but have been linked to the induction of thymic lymphoma. to assess the role of felv-b in the induction of multicentric lymphoma and other non-t-cell disease, the frequency of occurrence and nature of felv-b were examined in ... | 2010 | 20451235 |
| diamond blackfan anemia at the crossroad between ribosome biogenesis and heme metabolism. | diamond-blackfan anemia (dba) is a rare, pure red-cell aplasia that presents during infancy. approximately 40% of cases are associated with other congenital defects, particularly malformations of the upper limb or craniofacial region. mutations in the gene coding for the ribosomal protein rps19 have been identified in 25% of patients with dba, with resulting impairment of 18s rrna processing and 40s ribosomal subunit formation. moreover, mutations in other ribosomal protein coding genes account ... | 2010 | 20454576 |
| immunodeficiencies caused by infectious diseases. | immunodeficiencies caused by infectious agents may result from disruption of normal host barriers or dysregulation of cellular immunity, the latter serving to promote survival of the infectious agent through immune evasion. such infections may be followed by opportunistic infections with a variety of other microorganisms. classic infectious causes of immunodeficiency in companion animals are the immunodeficiency retroviruses, including feline immunodeficiency virus and feline leukemia virus. oth ... | 2010 | 20471525 |
| naturally acquired feline immunodeficiency virus (fiv) infection in cats from western canada: prevalence, disease associations, and survival analysis. | this retrospective study evaluated epidemiologic features and disease associations of feline immunodeficiency virus (fiv) infection in client owned cats from western canada. among 1205 cats that were tested 66 (5.5%) were positive for fiv antibody (fiv(+)) with a higher prevalence in males than females. fiv(+) cats were older than the overall population. epidemiologic features and disease associations were compared between 58 fiv(+), but feline leukemia virus negative (felv(-)) cats and 58 age a ... | 2010 | 20514250 |
| unexpected allelic heterogeneity and spectrum of mutations in fowler syndrome revealed by next-generation exome sequencing. | protein coding genes constitute approximately 1% of the human genome but harbor 85% of the mutations with large effects on disease-related traits. therefore, efficient strategies for selectively sequencing complete coding regions (i.e., "whole exome") have the potential to contribute our understanding of human diseases. we used a method for whole-exome sequencing coupling agilent whole-exome capture to the illumina dna-sequencing platform, and investigated two unrelated fetuses from nonconsangui ... | 2010 | 20518025 |
| light whole genome sequence for snp discovery across domestic cat breeds. | the domestic cat has offered enormous genomic potential in the veterinary description of over 250 hereditary disease models as well as the occurrence of several deadly feline viruses (feline leukemia virus--felv, feline coronavirus--fecv, feline immunodeficiency virus--fiv) that are homologues to human scourges (cancer, sars, and aids respectively). however, to realize this bio-medical potential, a high density single nucleotide polymorphism (snp) map is required in order to accomplish disease a ... | 2010 | 20576142 |
| polymerase chain reaction survey of feline haemoplasma infections in greece. | the aim of this study was to use real-time polymerase chain reaction assays to determine the prevalence of three haemoplasma species in cats from greece and to evaluate possible associations between haemoplasma infection and age, gender, feline immunodeficiency virus/feline leukaemia virus (fiv/felv) status and packed cell volume (pcv). ninety-seven cats (24 ill anaemic, 55 ill non-anaemic, 18 healthy non-anaemic) were included in the study. twenty cats (20.6%) were haemoplasma positive; seven c ... | 2010 | 20580298 |
| kinetics and specificity of feline leukemia virus subgroup c receptor (flvcr) export function and its dependence on hemopexin. | the feline leukemia virus subgroup c receptor (flvcr) is a heme export protein that is required for proerythroblast survival and facilitates macrophage heme iron recycling. however, its mechanism of heme export and substrate specificity are uncharacterized. using [(55)fe]heme and the fluorescent heme analog zinc mesoporphyrin, we investigated whether export by flvcr depends on the availability and avidity of extracellular heme-binding proteins. export was 100-fold more efficient when the medium ... | 2010 | 20610401 |
| does a feline leukemia virus infection pave the way for bartonella henselae infection in cats? | domestic cats serve as the reservoir hosts of bartonella henselae and may develop mild clinical symptoms or none after experimental infection. in humans, b. henselae infection can result in self-limiting cat scratch disease. however, immunocompromised patients may suffer from more-severe courses of infection or may even develop the potentially lethal disease bacillary angiomatosis. it was reasoned that cats with immunocompromising viral infections may react similarly to b. henselae infection. th ... | 2010 | 20610682 |
| malignant lymphoma in african lions (panthera leo). | malignant lymphoma has become an increasingly recognized problem in african lions (panthera leo). eleven african lions (9 male and 2 female) with clinical signs and gross and microscopic lesions of malignant lymphoma were evaluated in this study. all animals were older adults, ranging in age from 14 to 19 years. immunohistochemically, 10 of the 11 lions had t-cell lymphomas (cd3(+), cd79a(-)), and 1 lion was diagnosed with a b-cell lymphoma (cd3(-), cd79a(+)). the spleen appeared to be the prima ... | 2010 | 20610770 |
| evolutionary dynamics of endogenous feline leukemia virus proliferation among species of the domestic cat lineage. | endogenous feline leukemia viruses (enfelvs) occur in the germ lines of the domestic cat and related wild species (genus felis). we sequenced the long terminal repeats and part of the env region of enfelvs in domestic cats and five wild species. a total of 305 enfelv sequences were generated across 17 individuals, demonstrating considerable diversity within two major clades. distinct proliferations of enfelvs occurred before and after the black-footed cat diverged from the other species. diversi ... | 2010 | 20615520 |
| characterization of neutrophil extracellular traps in cats naturally infected with feline leukemia virus. | feline leukemia virus (felv), a common, naturally occurring gammaretrovirus in domestic cats, is associated with degenerative diseases of the haematopoietic system, immunodeficiency and neoplasia. felv infection causes an important suppression of neutrophil function, leading to opportunistic infections. recently, a new microbicidal mechanism named netosis was described in human, bovine and fish neutrophils, as well as in chicken heterophils. the purpose of the present study was to characterize n ... | 2010 | 19793908 |
| exposure of cats to low doses of felv: seroconversion as the sole parameter of infection. | in felids, feline leukemia virus (felv) infection results in a variety of outcomes that range from abortive (virus readily eliminated and never detectable) to progressive infection (persistent viremia and viral shedding). recently, a novel outcome was postulated for low felv infectious doses. naïve cats exposed to faeces of persistently infected cats seroconverted, indicating infection, but remained negative for provirus and p27 antigen in blood. felv provirus was found in some tissues but not i ... | 2010 | 19861115 |
| factors affecting seroprevalence of toxoplasma gondii in the endangered iberian lynx (lynx pardinus). | wild felids are considered important in maintaining the sylvatic cycle of toxoplasma gondii. although, t. gondii antibodies have been reported in several species of wild felids, little is known of the epidemiology and risk factors associated with t. gondii infection in wild cats. the iberian lynx (lynx pardinus) is the most endangered felid species in the world. in the present study, seroprevalence and associated risk factors for t. gondii infection in a large population of iberian lynx in spain ... | 2010 | 19879052 |
| molecular cytogenetic analysis of feline leukemia virus insertions in cat lymphoid tumor cells. | this study was conducted to map the acquired proviral insertions in the chromosomal genome of feline lymphoid tumors induced by feline leukemia virus (felv). chromosome specimens of the lymphoid tumor-derived cell lines and normal cat lymphocytes were subjected to fluorescence in situ hybridization and tyramide signal amplification, using an exogenous felv-a genome as a probe. specific hybridization signals were detected only on the metaphase chromosomes of the tumor cells. poisson's distributio ... | 2010 | 19879301 |
| fusogenic membrane glycoproteins induce syncytia formation and death in vitro and in vivo: a potential therapy agent for lung cancer. | fusogenic membrane glycoproteins (fmgs) are viral envelope proteins, which bind surface receptors and induce fusion of the cell membrane. an fmg-transfected cell will fuse with neighbor cells, thus forming syncytia that die within 5 days. in this report, plasmids encoding for fmgs from human endogenous retrovirus-w (herv-w) was compared with gibbon ape leukemia virus (galv) and feline endogenous virus rd-114 (rd). these plasmids were transfected in human non-small-cell lung cancer (nsclc) cells ... | 2010 | 19893593 |
| feline leukemia virus infection: a threat for the survival of the critically endangered iberian lynx (lynx pardinus). | the iberian lynx (lynx pardinus) is considered the most endangered felid species in the world. to date, less than 200 animals remain in the wild. low numbers and genetic uniformity may contribute to render this species particularly susceptible to infectious diseases. different pathogens have been identified in iberian lynxes; including several feline bacterial and viral agents. within a 6-month period starting in december 2006, 12 lynxes living in the northern part of the doñana area were found ... | 2010 | 19896221 |
| restriction of feline retroviruses: lessons from cat apobec3 cytidine deaminases and trim5alpha proteins. | the interplay between viral and cellular factors determines the outcome of an initial contact between a given virus and its natural host or upon encounter of a novel host. thus, the potential of inducing disease as well as crossing host species barriers are the consequences of the molecular interactions between the parasite and its susceptible, tolerant or resistant host. cellular restriction factors, for instance apobec3 and trim5 proteins, targeting defined pathogens or groups of pathogens as ... | 2010 | 19896724 |
| focus assay on felix-dependent feline leukemia virus. | t-lymphotropic feline leukemia virus (felv-t) induces immunodeficiency in cats. felv-t is fusion-defective and requires a cofactor, termed felix, for infection. felix is a truncated envelope glycoprotein of an endogenous felv and mediates infection by binding a phosphate transporter pit-1. in this study, we established a feline sarcoma-positive leukemia-negative cell line expressing felix, named qn/felix cells. upon infection, felv-t induced prominent foci with syncytia in qn/felix cells and cou ... | 2010 | 19915325 |
| role of latent feline leukemia virus infection in nonregenerative cytopenias of cats. | nonregenerative cytopenias such as nonregenerative anemia, neutropenia, and thrombocytopenia in cats with feline leukemia virus (felv) antigen are assumed to be caused by the underlying felv infection. in addition, cats with negative felv antigen-test results that have cytopenias of unknown etiology often are suspected to suffer from latent felv infection that is responsible for the nonregenerative cytopenias. | 2010 | 19925574 |
| evaluation of a new in-clinic test system to detect feline immunodeficiency virus and feline leukemia virus infection. | many in-house tests for the diagnosis of feline immunodeficiency virus (fiv) and feline leukemia virus (felv) infection are licensed for use in veterinary practice. a new test with unknown performance has recently appeared on the market. | 2010 | 19930142 |
| 9th international feline retrovirus research symposium 2008. preface. | 2010 | 19931187 | |
| apparent feline leukemia virus-induced chronic lymphocytic leukemia and response to treatment. | chylothorax secondary to chronic lymphocytic leukemia (cll) was diagnosed in a feline leukemia virus (felv)-positive 8-year-old castrated male domestic shorthair feline. the leukemia resolved following therapy with chlorambucil, prednisone, cyclophosphamide, doxorubicin, and lomustine. to our knowledge, this is the first reported case of cll in an felv-positive cat. although a causative relationship cannot be proven, patients diagnosed with either disease may benefit from diagnostics to rule out ... | 2010 | 19945894 |
| seroprevalences to viral pathogens in free-ranging and captive cheetahs (acinonyx jubatus) on namibian farmland. | cheetah populations are diminishing rapidly in their natural habitat. one reason for their decline is thought to be a high susceptibility to (infectious) diseases because cheetahs in zoos suffer from high disease-induced mortality. data on the health status of free-ranging cheetahs are scarce, and little is known about their exposure and susceptibility to infectious diseases. we determined seroprevalences to nine key viruses (feline herpesvirus 1, feline calicivirus, feline parvovirus, feline co ... | 2010 | 19955325 |
| feline leukemia virus immunity induced by whole inactivated virus vaccination. | a fraction of cats exposed to feline leukemia virus (felv) effectively contain virus and resist persistent antigenemia/viremia. using real-time pcr (qpcr) to quantitate circulating viral dna levels, previously we detected persistent felv dna in blood cells of non-antigenemic cats considered to have resisted felv challenge. in addition, previously we used rna qpcr to quantitate circulating viral rna levels and determined that the vast majority of viral dna is transcriptionally active, even in the ... | 2010 | 20004483 |
| chronic progressive polyarthritis in a female cat. | feline chronic progressive polyarthritis is a rare immune-mediated disease that has only previously been reported in male cats. a one-year-old female cat was presented with anorexia, lassitude and lameness. the tarsal, carpal and elbow joints revealed swelling, pain, stiffness, crepitus and regional lymphadenopathy, and fever was present. the cat was clinically diagnosed with chronic progressive polyarthritis based on the fever, swelling of joints, imaging of erosive proliferative periosteal pol ... | 2010 | 20009422 |
| coombs', haemoplasma and retrovirus testing in feline anaemia. | to investigate the associations between coombs' testing, haemoplasma and retroviral infections, and feline anaemia. | 2010 | 20070499 |
| differential diagnosis of feline leukemia virus subgroups using pseudotype viruses expressing green fluorescent protein. | feline leukemia virus (felv) is classified into three receptor interference subgroups, a, b and c. in this study, to differentiate felv subgroups, we developed a simple assay system using pseudotype viruses expressing green fluorescent protein (gfp). we prepared gfp pseudotype viruses, named gfp(felv-a), gfp(felv-b) and gfp(felv-c) harboring envelopes of felv-a, b and c, respectively. the gfp pseudotype viruses completely interfered with the same subgroups of felv reference strains on fea cells ... | 2010 | 20124765 |
| feline immunodeficiency virus, feline leukemia virus and bartonella species in stray cats on st kitts, west indies. | stray cats trapped in various areas of basseterre, the capital of st kitts in the west indies, were tested for infection with feline immunodeficiency virus (fiv) and feline leukemia virus (felv) using commercial kits. of 99 (51 male and 48 female) cats trapped in 2006/7, 15% (12 males and three females) were positive for fiv while none were positive for felv. of 72 (41 males and 31 females) cats trapped in 2009, 14% (nine males and one female) were positive for fiv while none were positive for f ... | 2010 | 20138558 |
| dominance of highly divergent feline leukemia virus a progeny variants in a cat with recurrent viremia and fatal lymphoma. | in a cat that had ostensibly recovered from feline leukemia virus (felv) infection, we observed the reappearance of the virus and the development of fatal lymphoma 8.5 years after the initial experimental exposure to felv-a/glasgow-1. the goals of the present study were to investigate this felv reoccurrence and molecularly characterize the progeny viruses. | 2010 | 20167134 |
| protection against feline leukemia virus challenge for at least 2 years after vaccination with an inactivated feline leukemia virus vaccine. | twelve cats were vaccinated at 8 and 11 weeks of age with a commercially available inactivated felv vaccine (nobivac felv, intervet/schering-plough animal health). eleven cats served as age-matched, placebo-vaccinated controls. all cats were kept in isolation for 2 years after vaccination and were then challenged with virulent felv to evaluate vaccine efficacy and duration of immunity. cats were monitored for 12 weeks after challenge for development of persistent viremia using a commercial felv ... | 2010 | 20957616 |
| prevalence of feline leukaemia provirus dna in feline lymphomas. | a significant drop in the prevalence of feline leukaemia virus (felv) antigenaemic cats and antigen-associated lymphomas has been observed after the introduction of felv vaccination and antigen-testing with removal of persistently antigenaemic cats. however, recent reports have indicated that regressively infected cats may contain felv provirus dna and that lymphoma development may be associated with the presence of provirus alone. in the present study, we investigated the presence of felv antig ... | 2010 | 21036089 |
| relevance of feline calicivirus, feline immunodeficiency virus, feline leukemia virus, feline herpesvirus and bartonella henselae in cats with chronic gingivostomatitis. | despite its common occurrence, the aetiology of chronic gingivostomatitis in cats remains uncertain. aetiology is likely multifactorial, and several infectious agents may be associated with chronic gingivostomatitis. the purpose of this study was to investigate the prevalence of feline calicivirus (fcv), feline immunodeficiency virus (fiv), feline leukemia virus (felv), feline herpesvirus (fhv), and bartonella henselae (b. henselae) in cats with chronic gingivostomatitis and in an age-matched co ... | 2010 | 21038808 |
| mutations in flvcr1 cause posterior column ataxia and retinitis pigmentosa. | the study of inherited retinal diseases has advanced our knowledge of the cellular and molecular mechanisms involved in sensory neural signaling. dysfunction of two specific sensory modalities, vision and proprioception, characterizes the phenotype of the rare, autosomal-recessive disorder posterior column ataxia and retinitis pigmentosa (pcarp). using targeted dna capture and high-throughput sequencing, we analyzed the entire 4.2 mb candidate sequence on chromosome 1q32 to find the gene mutated ... | 2010 | 21070897 |
| immunization with the transmembrane protein of a retrovirus, feline leukemia virus: absence of antigenemia following challenge. | a major challenge in the development of vaccines against retroviruses is the induction of neutralizing antibodies since they prevent infection of the cells where the virus may persist. the transmembrane envelope (tm) protein contains highly conserved domains and seems to be a suitable target. to study whether vaccinating with a tm protein of a retrovirus could protect from infection in vivo, cats were immunized with the tm protein p15e of feline leukemia virus (felv) and subsequently challenged. ... | 2010 | 21108970 |
| incidence of persistent viraemia and latent feline leukaemia virus infection in cats with lymphoma. | in the past, feline leukaemia virus (felv) infection, and also latent felv infection, were commonly associated with lymphoma and leukaemia. in this study, the prevalence of felv provirus in tumour tissue and bone marrow in felv antigen-negative cats with these tumours was assessed. seventy-seven diseased cats were surveyed (61 antigen-negative, 16 antigen-positive). blood, bone marrow, and tumour samples were investigated by two polymerase chain reaction (pcr) assays detecting deoxyribonucleic a ... | 2010 | 21131219 |
| bartonella species, feline herpesvirus-1, and toxoplasma gondii pcr assay results from blood and aqueous humor samples from 104 cats with naturally occurring endogenous uveitis. | toxoplasma gondii, bartonella henselae and feline herpesvirus-1 (fhv-1) have been implicated as causative agents in feline uveitis. the usefulness of serum and aqueous humor (ah) antibody testing for these agents is limited as antibodies can be detected in both healthy cats and cats with uveitis. very few studies using polymerase chain reaction (pcr) assays to amplify organism dna from samples from cats with uveitis have been performed. in this study, assays to detect t gondii antibodies, feline ... | 2010 | 20817587 |
| the fowler syndrome-associated protein flvcr2 is an importer of heme. | mutations in flvcr2, a cell surface protein related by homology and membrane topology to the heme exporter/retroviral receptor flvcr1, have recently been associated with fowler syndrome, a vascular disorder of the brain. we previously identified flvcr2 to function as a receptor for fy981 feline leukemia virus (felv). however, the cellular function of flvcr2 remains unresolved. here, we report the cellular function of flvcr2 as an importer of heme, based on the following observations. first, flvc ... | 2010 | 20823265 |
| myelodysplastic syndromes and acute myeloid leukemia in cats infected with feline leukemia virus clone33 containing a unique long terminal repeat. | feline leukemia virus (felv) clone33 was obtained from a domestic cat with acute myeloid leukemia (aml). the long terminal repeat (ltr) of this virus, like the ltrs present in felv from other cats with aml, differs from the ltrs of other known felv in that it has 3 tandem direct 47-bp repeats in the upstream region of the enhancer (ure). here, we injected cats with felv clone33 and found 41% developed myelodysplastic syndromes (mds) characterized by peripheral blood cytopenias and dysplastic cha ... | 2009 | 19035458 |
| a serological survey of common feline pathogens in free-living european wildcats (felis silvestris) in central spain. | twenty-five serum samples of 22 free-living european wildcats (felis silvestris) captured from 1991 to 1993 in central spain were tested for evidence of exposure to seven feline pathogens. all the wildcats but one (95.4%) presented evidence of contact with at least one of the agents (mean = 2.2). contact with feline leukemia virus (felv) was detected in 81% of the wildcats (antibodies, 77%; antigen p27, 15%). antibodies to feline calicivirus (fcv, 80%), feline herpesvirus (fhv, 20%), feline parv ... | 2009 | 32214938 |
| [receptors for animal retroviruses]. | diseases caused by animal retroviruses have been recognized since 19th century in veterinary field. most livestock and companion animals have own retroviruses. to disclose the receptors for these retroviruses will be useful for understanding retroviral pathogenesis, developments of anti-retroviral drugs and vectors for human and animal gene therapies. of retroviruses in veterinary field, receptors for the following viruses have been identified; equine infectious anemia virus, feline immunodefici ... | 2009 | 20218331 |
| improved health and survival of fiv-infected cats is associated with the presence of autoantibodies to the primary receptor, cd134. | we analyzed antibody responses in sera from feline immunodeficiency virus (fiv)-infected and uninfected cats. a strong antiviral response to the viral surface glycoprotein (su) was noted in both natural and experimental infections. in addition, 143 of 226 fiv-infected animals (63%) also expressed antibodies to the primary binding receptor, cd134, whereas cats infected with other feline rna viruses, including calicivirus, coronavirus, herpesvirus, and feline leukemia virus, did not. both affinity ... | 2009 | 19901342 |
| the kinetics of feline leukaemia virus shedding in experimentally infected cats are associated with infection outcome. | feline leukaemia virus (felv) infection in felids results mainly from oronasal exposure to infectious saliva and nasal secretions, but the potential for viral transmission through faeces and urine has not been completely characterized. in order to assess and compare potential felv transmission routes, we determined the viral kinetics in plasma, saliva, faeces and urine during early experimental felv infection (up to week 15 post-exposure) in specific pathogen-free cats. in addition to monitoring ... | 2009 | 18774240 |
| fecal shedding of infectious feline leukemia virus and its nucleic acids: a transmission potential. | although it is assumed that fecal shedding of feline leukemia virus (felv) constitutes a transmission potential, no study has been performed showing that feces of infected cats can be a source of infection. in this study, we investigated fecal viral shedding of felv and its role in viral pathogenesis with the goal to improve infection control. felv rna and dna levels were determined in rectal swabs of experimentally infected cats by real-time pcr, and the results were correlated with proviral an ... | 2009 | 18842363 |
| single-dose pharmacokinetics and genotoxicity of metronidazole in cats. | single-dose pharmacokinetics and genotoxicity of metronidazole in cats were evaluated. cats received either 5mg/kg metronidazole intravenously, or 20mg/kg metronidazole benzoate (12.4mg/kg metronidazole base) orally in a single dose. serial plasma samples were collected and assayed for metronidazole using high pressure liquid chromatography (hplc). genotoxicity was assessed in vitro in feline peripheral blood mononuclear cells (pbmc) and a feline t-cell lymphoma line incubated with metronidazole ... | 2009 | 19155181 |
| identification of a novel common proviral integration site, flit-1, in feline leukemia virus induced thymic lymphoma. | a new proviral integration site for feline leukemia virus (felv), termed flit-1, was identified from feline thymic lymphoma. among 35 felv-related tumors examined, 5 of 25 thymic lymphomas demonstrated proviral insertion within flit-1 locus whereas none of four alimentary and five multicentric lymphomas and one t-lymphoid leukemia examined had rearrangement in this region. extensive sequence analysis has shown that flit-1, which is noncoding, is conserved on human chromosome 12 and mouse chromos ... | 2009 | 19203775 |
| feline leukemia virus and other pathogens as important threats to the survival of the critically endangered iberian lynx (lynx pardinus). | background: the iberian lynx (lynx pardinus) is considered the most endangered felid species in the world. in order to save this species, the spanish authorities implemented a captive breeding program recruiting lynxes from the wild. in this context, a retrospective survey on prevalence of selected feline pathogens in free-ranging lynxes was initiated. methodology/ principal findings: we systematically analyzed the prevalence and importance of seven viral, one protozoan (cytauxzoon felis), and s ... | 2009 | 19270739 |
| single-round selection yields a unique retroviral envelope utilizing gpr172a as its host receptor. | the recognition by a viral envelope of its cognate host-cell receptor is the initial critical step in defining the viral host-range and tissue specificity. this study combines a single-round of selection of a random envelope library with a parallel cdna screen for receptor function to identify a distinct retroviral envelope/receptor pair. the 11-aa targeting domain of the modified feline leukemia virus envelope consists of a constrained peptide. critical to the binding of the constrained peptide ... | 2009 | 19307586 |
| identification of ltr-specific small non-coding rna in felv infected cells. | the u3-ltr region of leukemia viruses transactivates cancer-related signaling pathways through the production of a non-coding rna transcript although the role of this transcript in virus infection remains unknown. in this study we demonstrate for the first time that an long terminal repeat (ltr)-specific small non-coding rna is produced from a feline leukemia virus (felv)-infected feline cell line. rna cloning identified this as a 104 base transcript that originates from the u3-ltr region. we al ... | 2009 | 19336234 |
| identification of a feline leukemia virus variant that can use thtr1, flvcr1, and flvcr2 for infection. | the pathogenic subgroup c feline leukemia virus (felv-c) arises in infected cats as a result of mutations in the envelope (env) of the subgroup a felv (felv-a). to better understand emergence of felv-c and potential felv intermediates that may arise, we characterized felv env sequences from the primary fy981 felv isolate previously derived from an anemic cat. here, we report the characterization of the novel fy981 felv env that is highly related to felv-a env but whose variable region a (vra) re ... | 2009 | 19369334 |
| seroprevalence of toxoplasma gondii and concurrent bartonella spp., feline immunodeficiency virus, and feline leukemia virus infections in cats from grenada, west indies. | toxoplasma gondii and bartonella spp. are zoonotic pathogens of cats. feline immunodeficiency virus (fiv) and feline leukemia virus (felv) are related to human immunodeficiency virus, and human leukemia virus, respectively; all of these viruses are immunosuppressive. in the present study, the prevalence of antibodies to t. gondi, bartonella spp., fiv, as well as felv antigen were determined in sera from 75 domestic and 101 feral cats (felis catus) from the caribbean island of grenada, west indie ... | 2009 | 19385716 |
| seroprevalence of viral infections in domestic cats in costa rica. | a cross-sectional survey of a convenient sample of domestic cats from costa rica's greater metropolitan area was carried out to determine the prevalence of antibodies against feline herpesvirus type 1 (fhv-1), feline parvovirus (fpv), feline immunodeficiency virus (fiv) and antigens of feline leukemia virus (felv). blood samples were collected from at least 96 cats from june 1998 to december 2001; data related to the individual cats and household variables were obtained using a questionnaire. an ... | 2009 | 19498296 |
| survey of feline leukemia virus and feline coronaviruses in captive neotropical wild felids from southern brazil. | a total of 57 captive neotropical felids (one leopardus geoffroyi, 14 leopardus pardalis, 17 leopardus wiedii, 22 leopardus tigrinus, and three puma yagouaroundi) from the itaipu binacional wildlife research center (refúgio bela vista, southern brazil) were anesthetized for blood collection. feces samples were available for 44 animals, including one l. geoffroyi, eight l. pardalis, 14 l. wiedii, 20 l. tigrinus, and one p. yagouaroundi. total dna and rna were extracted from blood and feces, respe ... | 2009 | 19569487 |
| prevalence of feline immunodeficiency virus and feline leukaemia virus among client-owned cats and risk factors for infection in germany. | this study was conducted to determine prevalence and risk factors for retrovirus infection of infected cats in a large cat population in germany by evaluation of 17,462 client-owned cats that were tested for the presence of feline immunodeficiency virus (fiv) antibodies or feline leukaemia virus (felv) antigen. the owners of a subset of 100 cats were contacted to determine their cat's survival times. prevalence of fiv and felv was 3.2% and 3.6%, respectively, remaining stable for fiv, but decrea ... | 2009 | 19616984 |
| hematology and serum biochemistry of feline immunodeficiency virus-infected and feline leukemia virus-infected cats. | hematological and biochemical values in cats naturally infected by feline immunodeficiency virus (fiv) or feline leukemia virus (felv) are not completely documented. | 2009 | 19645840 |
| new structural arrangement of the extracellular regions of the phosphate transporter slc20a1, the receptor for gibbon ape leukemia virus. | infection of a host cell by a retrovirus requires an initial interaction with a cellular receptor. for numerous gammaretroviruses, such as the gibbon ape leukemia virus, woolly monkey virus, feline leukemia virus subgroup b, feline leukemia virus subgroup t, and 10a1 murine leukemia virus, this receptor is the human type iii sodium-dependent inorganic phosphate transporter, slc20a1, formerly known as pit1. understanding the critical receptor functionalities and interactions with the virus that l ... | 2009 | 19717569 |
| seroprevalence of feline leukemia virus and feline immunodeficiency virus infection among cats in canada. | the purposes of this study were to determine the seroprevalence of feline leukemia virus (felv) and feline immunodeficiency virus (fiv) infection among cats in canada and to identify risk factors for seropositivity. signalment, lifestyle factors, and test results for felv antigen and fiv antibody were analyzed for 11 144 cats from the 10 canadian provinces. seroprevalence for fiv antibody was 4.3% and seroprevalence for felv antigen was 3.4%. fifty-eight cats (0.5%) were seropositive for both vi ... | 2009 | 19721785 |
| disease threats to the endangered iberian lynx (lynx pardinus). | the iberian lynx, (lynx pardinus), is the most endangered felid in the world. to determine whether sympatric carnivores are reservoirs of pathogens posing a disease risk for the lynx, evidence of exposure to 17 viral, bacterial and protozoan agents was investigated in 176 carnivores comprising 26 free-living lynx, 53 domestic cats, 28 dogs, 33 red foxes (vulpes vulpes), 24 egyptian mongooses (herpestes ichneumon), 10 common genets (genetta genetta) and 2 eurasian badgers (meles meles) in the are ... | 2009 | 18555712 |
| serological survey of toxoplasma gondii infection in domestic cats from northeastern portugal. | cats are very important hosts in the epidemiological cycle of toxoplasma gondii, a zoonotic protozoan parasite that can infect humans and many other animal species worldwide. we report a serological survey of antibodies to t. gondii in domestic cats from northeastern portugal, by means of the modified agglutination test. three cats had titres of 20 (3.9%), 18 had titres of 40 (23.7%) and 55 animals had titres of > or =800 (72.4%). results of three seropositive kittens with less than 4 months wer ... | 2008 | 18571327 |
| prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in rio de janeiro--brazil. | the aim of this study was to determine the prevalence and risk factors for mycoplasma haemofelis (mhf) and 'candidatus mycoplasma haemominutum' (mhm) infections in domestic cats tested for feline immunodeficiency virus (fiv) and feline leukemia virus (felv) with a commercial enzyme-linked immunosorbent assay (elisa) kit. based on serological testing, cats were grouped as i) fiv-positive (n=25); ii) felv-positive (n=39); iii) fiv/felv-positive (n=8); and iv) fiv/felv-negative (n=77). complete blo ... | 2008 | 17905624 |
| evaluation of the association of bartonella species, feline herpesvirus 1, feline calicivirus, feline leukemia virus and feline immunodeficiency virus with chronic feline gingivostomatitis. | gingivostomatitis (gs) is a significant condition in cats because of oral discomfort and associated periodontal disease. several infectious agents have been associated with the presence of gs, but a causal relationship is unclear. the cats in this study were housed together, had a history of flea exposure, and were vaccinated with a modified live fvrcp product. there were nine cats with active gs and 36 unaffected cats at the time of sample collection. serum was tested for feline leukemia virus ... | 2008 | 17766156 |
| the c domain in the surface envelope glycoprotein of subgroup c feline leukemia virus is a second receptor-binding domain. | the receptor-binding domain (rbd) in the surface (su) subunit of gammaretrovirus envelope glycoprotein is critical for determining the host receptor specificity of the virus. this domain is separated from the carboxy terminal c domain (cdom) of su by a proline-rich region. in this study, we show that the cdom region in the su from subgroup c feline leukemia virus (felv-c) forms a second receptor-binding domain that is distinct from its rbd, and which can independently bind to its host receptor f ... | 2008 | 17945326 |
| genetic characterization of feline leukemia virus from florida panthers. | from 2002 through 2005, an outbreak of feline leukemia virus (felv) occurred in florida panthers (puma concolor coryi). clinical signs included lymphadenopathy, anemia, septicemia, and weight loss; 5 panthers died. not associated with felv outcome were the genetic heritage of the panthers (pure florida vs. texas/florida crosses) and co-infection with feline immunodeficiency virus. genetic analysis of panther felv, designated felv-pco, determined that the outbreak likely came from 1 cross-species ... | 2008 | 18258118 |
| a heme export protein is required for red blood cell differentiation and iron homeostasis. | hemoproteins are critical for the function and integrity of aerobic cells. however, free heme is toxic. therefore, cells must balance heme synthesis with its use. we previously demonstrated that the feline leukemia virus, subgroup c, receptor (flvcr) exports cytoplasmic heme. here, we show that flvcr-null mice lack definitive erythropoiesis, have craniofacial and limb deformities resembling those of patients with diamond-blackfan anemia, and die in midgestation. mice with flvcr that is deleted n ... | 2008 | 18258918 |
| infectious diseases of dogs and cats on isabela island, galapagos. | vaccination and importation of dogs and cats are prohibited in the galapagos, resulting in a uniquely isolated population. the purpose of this study was to determine the prevalence of infectious diseases of dogs and cats that impact their health, could spill over to native wildlife, or sentinel diseases of concern to humans. | 2008 | 18289290 |
| advances in understanding molecular determinants in felv pathology. | feline leukemia virus (felv) occurs in nature not as a single genomic species but as a family of closely related viruses. the disease outcome of natural felv infection is variable and likely reflects genetic variation both in the virus and the naturally outbreeding host population. a series of studies have been undertaken with the objectives of examining natural felv genetic variation, the selective pressures operative in felv infection that lead to predominance of natural variants, and the cons ... | 2008 | 18289704 |
| quantification of endogenous and exogenous feline leukemia virus sequences by real-time pcr assays. | endogenous retroviruses are integrated in the genome of most vertebrates. they represent footprints of ancient retroviral infection and are vertically transmitted from parents to their offspring. in the genome of all domestic cats, sequences closely related to exogenous felv known as endogenous feline leukemia virus (enfelv), are present. enfelv are incapable of giving rise to infectious virus particles. however, transcription and translation of enfelv have been demonstrated in tissues of health ... | 2008 | 18295344 |
| how molecular methods change our views of felv infection and vaccination. | felv was discovered 40 years ago and vaccines have been commercially available for almost two decades. so far, most felv pathogenesis and vaccine studies were conducted assaying parameters, such as virus isolation and antigen detection. accordingly, regressive infection was characterized by transient or undetectable viremia, while persistent viremia is typically observed in cats with progressive infection. using real-time polymerase chain reaction assays, the spectrum of host response categories ... | 2008 | 18295346 |
| acute monoblastic leukemia in a felv-positive cat. | a 1.6-year-old male domestic short hair cat was brought to the veterinary medical teaching hospital, kasetsart university, with signs of severe anemia, depression, and general lymph node enlargement. complete blood count revealed leukocytosis and massive undifferentiated blasts. testing for antibodies specific to feline leukemia virus (felv) was positive, and felv nucleic acid was confirmed by nested polymerase chain reaction. base on cytochemistry and ultrastructure, the cat was diagnosed with ... | 2008 | 18296895 |
| real-time pcr investigation of feline leukemia virus proviral and viral rna loads in leukocyte subsets. | cats exposed to feline leukemia virus (felv), a naturally occurring gammaretrovirus develop either progressive or regressive infection. recent studies using analyses with enhanced sensitivity have correlated loads throughout felv with the clinical outcome, though remarkably, during the acute phase of infection, proviral and viral rna burdens in the peripheral blood do not differ between groups. we hypothesized that viral loads in specific leukocyte subsets influence the infection outcome. using ... | 2008 | 18304650 |
| molecular pathogenesis of feline leukemia virus-induced malignancies: insertional mutagenesis. | feline leukemia virus (felv), which is subclassified into three subgroups of a, b and c, is a pathogenic retrovirus in cats. felv-a is minimally pathogenic, felv-c can cause pure red cell aplasia, and felv-b is associated with a variety of pathogenic properties such as lymphoma, leukemia and anemia. felv-induced neoplasms are caused, at least in part, by somatically acquired insertional mutagenesis in which the integrated provirus may activate a proto-oncogene or disrupt a tumor suppressor gene. ... | 2008 | 18313764 |
| functions, structure, and read-through alternative splicing of feline apobec3 genes. | over the past years a variety of host restriction genes have been identified in human and mammals that modulate retrovirus infectivity, replication, assembly, and/or cross-species transmission. among these host-encoded restriction factors, the apobec3 (a3; apolipoprotein b mrna-editing catalytic polypeptide 3) proteins are potent inhibitors of retroviruses and retrotransposons. while primates encode seven of these genes (a3a to a3h), rodents carry only a single a3 gene. | 2008 | 18315870 |
| development and application of a quantitative real-time pcr assay to detect feline leukemia virus rna. | we previously defined four categories of feline leukemia virus (felv) infection, designated as abortive, regressive, latent, and progressive. to determine if detectable viral dna is transcriptionally active in the absence of antigenemia, we developed and validated a real-time viral rna qpcr assay. this assay proved to be highly sensitive, specific, reproducible, and allowed reliable quantitation. we then applied this methodology, together with real-time dna qpcr and p27 capsid antigen capture el ... | 2008 | 18321595 |
| [felv infection, the kiss of death]. | 2008 | 18384012 | |
| association between endogenous feline leukemia virus loads and exogenous feline leukemia virus infection in domestic cats. | recently, we demonstrated that endogenous feline leukemia virus (enfelv) loads may vary among cats of different populations and that felv-infected cats have higher enfelv loads than uninfected cats. thus, we hypothesized that enfelv might influence the pathogenesis and outcome of felv infection. no significant difference in the infection outcome (regressive versus progressive infection) was observed between groups of cats with high or low enfelv loads following felv-a challenge. however, cats wi ... | 2008 | 18407367 |
| seroprevalences of feline leukemia virus and feline immunodeficiency virus in cats with abscesses or bite wounds and rate of veterinarian compliance with current guidelines for retrovirus testing. | to determine the seroprevalences of and seroconversion rates for felv and fiv infection in cats treated for bite wounds and cutaneous abscesses and to evaluate compliance with recommendations to determine the retrovirus infection status of cats at acquisition and 60 days after a high-risk event. | 2008 | 18412524 |
| combined administration in a single injection of a feline multivalent modified live vaccine against fhv, fcv, and fplv together with a recombinant felv vaccine is both safe and efficacious for all four major feline viral pathogens. | nobivac tricat, a lyophilised trivalent modified live attenuated vaccine is routinely used to protect cats against three commonly diagnosed feline viral pathogens namely herpesvirus, calicivirus and panleukopenia virus. the recognition of feline leukaemia virus (felv) as an important viral pathogen has prompted the development of an efficacious liquid recombinant subunit felv vaccine (p45 envelope protein). lyophilised tricat vaccine was dissolved in the liquid felv vaccine and no detectable del ... | 2008 | 18448375 |
| 2008 american association of feline practitioners' feline retrovirus management guidelines. | feline leukemia virus (felv) and feline immunodeficiency virus (fiv) are among the most common infectious diseases of cats. although vaccines are available for both viruses, identification and segregation of infected cats form the cornerstone for preventing new infections. guidelines in this report have been developed for diagnosis, prevention, treatment, and management of felv and fiv infections. all cats should be tested for felv and fiv infections at appropriate intervals based on individual ... | 2008 | 18455463 |
| detection of feline leukemia virus in the endangered iberian lynx (lynx pardinus). | feline retroviruses are rarely reported in lynx species. twenty-one iberian lynx (lynx pardinus) blood and tissue samples collected from doñana national park and los villares (sierra morena) in southern spain during 1993-2003 were analyzed by polymerase chain reaction to amplify nucleic acids from feline retroviruses. six samples were positive for feline leukemia virus (felv), but no samples tested positive for feline immunodeficiency virus. the blast analysis indicated that 5 of the 6 sequences ... | 2008 | 18460634 |
| retroviral infections of small animals. | retroviral infections are particularly important in cats, which are commonly infected with feline leukemia virus and feline immunodeficiency virus. this article describes the biology of these viruses and explores current issues regarding vaccination and diagnosis. the seeming lack of a recognized retrovirus infection in dogs is speculated on, and current and potential future therapies are discussed. | 2008 | 18501285 |
| compatibility between a rabies vaccine and a combined vaccine against feline rhinotracheitis, fcv, fplv and felv, and chlamydophila felis. | 2008 | 18503070 | |
| incidence of positive heartworm antibody and antigen tests at idexx laboratories: trends and potential impact on feline heartworm awareness and prevention. | data from the idexx laboratories reference laboratory network were retrospectively examined for feline heartworm testing trends in testing frequency, geographic bias, and prevalence for the years 2000--2006. examination of the data supports the commonly held view that veterinarians do not embrace heartworm disease testing or prevention in cats to the same degree they do in dogs. despite significant awareness and adoption of heartworm testing and prevention in dogs, we hypothesized that heartworm ... | 2008 | 18922639 |
| naturally occurring feline leukemia virus subgroup a and b infections in urban domestic cats. | a nested-pcr (n-pcr) was used to detect feline leukemia virus (felv) proviral dna in blood samples from 464 sick and 608 healthy domestic cats (felis catus) selected by convenience, and a significantly high prevalence of felv infection was observed. n-pcr results revealed the presence of felv proviral dna in 47.2 % of sick cats and 47.4 % of healthy cats. phylogenetic analysis revealed that felv samples from healthy or sick cats were grouped into separate clades. we determined felv subgroups by ... | 2008 | 18931078 |
| epizootiology and management of feline leukemia virus in the florida puma. | feline leukemia virus (felv) was not detected in florida pumas (puma concolor coryi) in almost 20 yr of surveillance; however, the finding of two felv antigen-positive pumas during the 2002-2003 capture season led to an investigation of felv in the population. between january 1990 and april 2007, the proportion of pumas testing felv antibody positive increased, with antibody-positive pumas concentrated in the northern portion of puma range. five of 131 (4%) pumas sampled between july 2000 and ap ... | 2008 | 18689639 |
| ocelots on barro colorado island are infected with feline immunodeficiency virus but not other common feline and canine viruses. | transmission of pathogens from domestic animals to wildlife populations (spill-over) has precipitated local wildlife extinctions in multiple geographic locations. identifying such events before they cause population declines requires differentiating spillover from endemic disease, a challenge complicated by a lack of baseline data from wildlife populations that are isolated from domestic animals. we tested sera collected from 12 ocelots (leopardus pardalis) native to barro colorado island, panam ... | 2008 | 18689668 |
| absolute quantitation of feline leukemia virus proviral dna and viral rna loads by taqman real-time pcr and rt-pcr. | sensitive taqman real-time polymerase chain reaction (pcr)-based methods have been developed recently for the detection and quantitation of feline leukemia virus (felv) proviral dna in infected cats. in this chapter, we outline the design and implementation of a taqman real-time pcr assay to quantify total felv proviral and viral rna loads in infected cats. the assay is designed to amplify all three felv subtypes (a-c), but not felv-related endogenous retroviral sequences. the system is tested a ... | 2008 | 18695960 |
| erythrophagocytic low-grade extranodal t-cell lymphoma in a cat. | a 13-year-old male castrated domestic shorthair cat was presented to the referring veterinarian with a 2-month history of weight loss and lethargy. splenomegaly, hepatomegaly, nonregenerative anemia, neutropenia, and hyperbilirubinemia were noted. results of testing for feline immunodeficiency virus, feline leukemia virus, toxoplasma gondii, and mycoplasma sp. were negative. on cytologic examination of aspirates from the enlarged spleen and liver, a population of erythrophagocytic round cells wa ... | 2008 | 19055577 |
| the location in cartilage of infectious retrovirus in cats infected with feline leukemia virus. | previous studies have suggested that articular cartilage allografts were not likely to transmit infectious retrovirus since viral dna could not be isolated from chondrocytes of infected individuals. however, the ability of the extracellular matrix of articular cartilage to harbor and transmit a retrovirus has not been examined. we hypothesized that articular cartilage fragments, but not isolated chondrocytes, from cats systemically infected with feline leukemia virus (felv) are capable of transm ... | 2007 | 17768202 |
| transduction of human hematopoietic stem cells by lentiviral vectors pseudotyped with the rd114-tr chimeric envelope glycoprotein. | lentiviral vectors are efficiently pseudotyped with rd114-tr, a chimeric envelope glycoprotein made of the extracellular and transmembrane domains of the feline leukemia virus rd114 and the cytoplasmic tail of the murine leukemia virus amphotropic envelope. rd114-tr-pseudotyped vectors may be concentrated by centrifugation, are resistant to complement inactivation, and are suitable for both ex vivo and in vivo gene therapy applications. we analyzed rd114-tr-pseudotyped, hiv-1-derived lentiviral ... | 2007 | 17824830 |