Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| management of spotted wilt vectored by frankliniella fusca (thysanoptera: thripidae) in virginia market-type peanut. | field tests were conducted during 2001 and 2002 in northeastern north carolina to evaluate the impact of cultural practices and in-furrow insecticides on the incidence of tomato spotted wilt virus (genus tospovirus, family bunyaviridae, tswv), which is transmitted to peanut, arachis hypogaea l., primarily by tobacco thrips, frankliniella fusca hinds (thysanoptera: thripidae). treatments included in row plant populations of 7, 13, and 17 plants per meter; the virginia market-type 'nc v-11' and 'p ... | 2005 | 16334308 |
| tomato spotted wilt virus particle assembly and the prospects of fluorescence microscopy to study protein-protein interactions involved. | 2005 | 16387194 | |
| [some biologic characteristics of two tomato spotted wilt virus isolates]. | biological properties of two tomato spotted wilt virus (tswv) isolates from the tomatoes, grown on plantations of the crimean tobacco-plant experimental stations have been investigated. these isolates were slightly differentiated by thermal inactivation point, by terms of virus storage in juice and by dilution end point. as a result the growth of the induced resistance effective with respect to tmv was observed in tobacco-plants of immunny-580 and n. sanderae varieties affected by tswv. | 2005 | 16396117 |
| host plant, temperature, and photoperiod effects on ovipositional preference of frankliniella occidentalis and frankliniella fusca (thysanoptera: thripidae). | host plant effects of tomato, lycopersicon esculentum mill., and chickweed, stellaria media (l.) vill., foliage infected and uninfected with tomato spotted wilt virus (family bunyaviridae, genus tospovirus, tswv) on the ovipositional preferences of western flower thrips, frankliniella occidentalis (pergande), and tobacco thrips, frankliniella fusca (hinds), were investigated for whole plants in the greenhouse. in addition, the preference for leaf disks from the same host plants was investigated ... | 2005 | 16539139 |
| first report of occurrence of two viruses on pea field in iran. | an intensive survey was conducted to identify virus diseases affecting pea crops in tehran province of iran. a total of 270 pea samples were collected randomly from pea fields. samples were tested by double antibody sandwich enzyme linked immunosorbent assay (das-elisa) using polyclonal antisera prepared against psbmv (as-0129, dsmz, braunschweig, germany) and tswv (as-0580, dsmz, braunschweig, germany). virus disease incidence in pea samples was followed by psbmv (33%) tswv (24.4%) and psbmv+ts ... | 2005 | 16637207 |
| the two envelope membrane glycoproteins of tomato spotted wilt virus show differences in lectin-binding properties and sensitivities to glycosidases. | tomato spotted wilt virus (tswv, genus: tospovirus, family: bunyaviridae) is a major constraint to the production of several different crops of agronomic and horticultural importance worldwide. the amino acid sequence of the two envelope membrane glycoproteins, designated as g(n) (n-terminal) and g(c) (c-terminal), of tswv contain several tripeptide sequences, asn-xaa-ser/thr, suggesting that the proteins are n-glycosylated. in this study, the lectin-binding properties of the viral glycoproteins ... | 2004 | 14967492 |
| using epidemiological information to develop effective integrated virus disease management strategies. | virus diseases cause serious losses in yield and quality of cultivated plants worldwide. these losses and the resulting financial damage can be limited by controlling epidemics using measures that minimise virus infection sources or suppress virus spread. for each combination of virus, cultivated plant and production system, there is an 'economic threshold' above which the financial damage is sufficient to justify using such measures. however, individual measures used alone may bring only small ... | 2004 | 15036832 |
| in vitro transcription of tomato spotted wilt virus is independent of translation. | ongoing transcription in vitro of tomato spotted wilt virus (tswv) has previously been demonstrated to require the presence of reticulocyte lysate. this dependence was further investigated by testing the occurrence of transcription in the presence of two translation inhibitors: edeine, an inhibitor that still allows scanning of nascent mrnas by the 40s ribosomal subunit, and cycloheximide, an inhibitor that completely blocks translation including ribosome scanning. neither of these inhibitors bl ... | 2004 | 15105551 |
| the plant virus tomato spotted wilt tospovirus activates the immune system of its main insect vector, frankliniella occidentalis. | tospoviruses have the ability to infect plants and their insect vectors. tomato spotted wilt virus (tswv), the type species in the tospovirus genus, infects its most important insect vector, frankliniella occidentalis, the western flower thrips (wft). however, no detrimental effects on the life cycle or cytopathological changes have been reported in the wft after tswv infection, and relatively few viral particles can be observed even several days after infection. we hypothesized that tswv infect ... | 2004 | 15113877 |
| [pathogenesis and induced virus resistance in tobacco plants affected by tomato spotted wilt virus]. | pathogenecity and virulence of tomato spotted wilt virus isolated from tobacco were studied under the field conditions of the crimea in the tobacco plants of immunny 580 variety and datura stramonium. as to their virulence the obtained 26 isolates were divided into three groups: strongly virulent (severe), middle-virulent and weakly virulent ("mosaic"). it has been shown that strongly virulent isolates interfere with weakly virulent ones and induce the development of nonspecific resistance in to ... | 2004 | 15208858 |
| host-associated genetic differentiation in thrips tabaci (insecta; thysanoptera), as determined from mtdna sequence data. | we tested for host-associated genetic differentiation in 22 populations of thrips tabaci collected from tobacco and leek, respectively. clustering analyses and haplotype networks based on sequence variation at a fragment of the mitochondrial cytochrome oxidase i gene yielded three major evolutionary lineages; two were clearly associated with leek and the third with tobacco. these genetic findings corroborated recent experimental observations on the heterogeneity of t. tabaci populations with reg ... | 2004 | 15241445 |
| trapping of tomato yellow leaf curl virus (tylcv) and other plant viruses with a groel homologue from the whitefly bemisia tabaci. | to avoid destruction in the haemolymph of their vector, many plant circulative viruses interact with groel homologues produced by insect endosymbiotic bacteria. we have exploited this phenomenon to devise tools allowing trapping of plant viruses by either groel purified from the whitefly bemisia tabaci or by whitefly groel over-expressed in e. coli. pcr tubes or 96-well plates coated with a groel preparation were incubated with cleared sap of virus infected plant leaves or insect vectors. groel- ... | 2004 | 15290375 |
| development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum. | an immunocapture reverse transcription loop-mediated isothermal amplification (ic/rt-lamp) was developed for the detection of tomato spotted wilt virus (tswv) from chrysanthemum. this method enabled sensitive, reproducible and specific detection of tswv from chrysanthemum plants. in the rt-lamp method, tswv genomic rna could be amplified under isothermal (65 degrees c) conditions within 1 h. the resulting amplicons were detected by the measurement or observation of the turbidity of the reaction ... | 2004 | 15350732 |
| specificity of accumulation and transmission of tomato spotted wilt virus (tswv) in two genera, frankliniella and thrips (thysanoptera: thripidae). | the accumulation and transmission of tomato spotted wilt virus (tswv) was examined in second instar larvae and adults of two thrips genera, frankliniella and thrips. the species tested were f. occidentalis (pergande), f. intonsa (trybom), t. tabacilindeman, t. setosus moulton, t. palmi karny and t. hawaiiensis (morgan). in a standard petunia leaf disc assay, the efficiencies of tswv transmission by two species of frankliniella were higher than those of any thrips species in the adult stage. a tr ... | 2004 | 15541189 |
| expression and characterization of a soluble form of tomato spotted wilt virus glycoprotein gn. | tomato spotted wilt virus (tswv), a member of the tospovirus genus within the bunyaviridae, is an economically important plant pathogen with a worldwide distribution. tswv is transmitted to plants via thrips (thysanoptera: thripidae), which transmit the virus in a persistent propagative manner. the envelope glycoproteins, g(n) and g(c), are critical for the infection of thrips, but they are not required for the initial infection of plants. thus, it is assumed that the envelope glycoproteins play ... | 2004 | 15542672 |
| sequence analysis of the glycoproteins of tomato chlorotic spot virus and groundnut ringspot virus and comparison with other tospoviruses. | the tospoviruses tomato chlorotic spot virus (tcsv) and groundnut ringspot virus (grsv) cause high economic losses in several vegetable crops in brazil. the glycoprotein precursor coding sequence was still not available for these two viruses. in this study, the 3' 4 kb m rna of tcsv and grsv genome was cloned and sequenced. the sequences were compiled with the available 5' region sequence (nsm gene and 5' utr) of the same isolates. the m rna of tcsv was deduced as formed by 4,882 nucleotides, wh ... | 2004 | 15550772 |
| tactics for management of thrips (thysanoptera: thripidae) and tomato spotted wilt virus in tomato. | four studies were conducted in georgia during spring 1999, 2000, 2001, and 2002 to evaluate various management tactics for reducing thrips and thrips-vectored tomato spotted wilt virus (tswv) in tomato and their interactions relative to fruit yield. populations of thrips vectors of tswv, frankliniella occidentalis (pergande) and frankliniella fusca (hinds), were determined using flower and sticky trap samples. the management practices evaluated were host plant resistance, insecticide treatments, ... | 2004 | 15568355 |
| occurrence and distribution of lettuce mosaic disease in tehran province from iran. | lettuce mosaic virus (lmv) cucumber mosaic virus (cmv) and tomato spotted wilt virus (tswv) were identified in fields of tehran province. in this study 452 leaf samples were collected from the fields throughout the tehran province during 2002 and 2003. distribution of lettuce mosaic virus (lmv), cucumber mosaic virus (cmv), tomato spotted wilt virus (tswv)and arabis mosaic virus (armv) was determined with das-elisa. percentage of single infection to lmv. cmv or tswv was 20.58, 15.93 and 9.96% re ... | 2004 | 15756832 |
| experimental and natural weed host-virus relations. | weeds, as alternative hosts of plant viruses and nutrient plants of virus vectors play important role in virus ecology and epidemiology. the aim of our study was to discover new weed-virus relations. therefore some weed species were mechanically inoculated with 28 viruses (strains or isolates) maintained in our glasshouse. different weed species with and without visible symptoms were collected from agro-, water ecosystems and wastelands of hungary between 1997 and 2003. virus infections were eva ... | 2004 | 15759395 |
| acquisition of tomato spotted wilt virus by adults of two thrips species. | abstract only larval thrips that acquire tomato spotted wilt virus (tswv), or adults derived from such larvae, transmit the virus. nonviruliferous adults can ingest virus particles while feeding on tswv-infected plants, but such adult thrips have not been shown to transmit tswv. immunofluorescence microscopy was used to show that thrips 1, 5, 10, and 20 days after adult emergence (dae) fed on tswv-infected plants acquired tswv with virus replication and accumulation occurring in both epithelial ... | 2004 | 18944108 |
| tomato spotted wilt virus infection improves host suitability for its vector frankliniella occidentalis. | abstract the effect of tomato spotted wilt virus (tswv) infection on plant attractiveness for the western flower thrips (frankliniella occidentalis) was studied. significantly more thrips were recovered on infected than were recovered on noninfected pepper (capsicum annuum) plants in different preference tests. in addition, more offspring were produced on the virus-infected pepper plants, and this effect also was found for tswv-infected datura stramonium. thrips behavior was minimally influenced ... | 2004 | 18943902 |
| interaction between tomato spotted wilt virus n protein monomers involves nonelectrostatic forces governed by multiple distinct regions in the primary structure. | abstract the ambisense rna genome of tomato spotted wilt virus (tswv) isby interaction with numerous copies of the virus encoded nucleocapsid (n) protein to form a subvirion structure called a ribonucleo-protein (rnp). rnps are central to the viral replication cycle because they, and not free viral rna, serve as templates for viral gene expression and genome replication. n protein monomers bind to viral rna molecules in a cooperative manner. we have examined regions of the n protein that are inv ... | 2004 | 18943909 |
| expression of a modified nucleocapsid-protein of tomato spotted wilt virus (tswv) confers resistance against tswv and groundnut ringspot virus (grsv) by blocking systemic spread. | summary tobacco transformed with a construct comprising the nucleocapsid-protein (n) gene of tomato spotted wilt virus (tswv) and the 5' non-translated leader sequence of plum pox virus (ppv) as a translation enhancer displays unusually broad resistance against tospoviruses. contrary to expectations, the transgenic plants did not express a higher level of tswv n protein than control plants harbouring the n gene without the ppv enhancer. instead, expression of an n-terminally elongated n protein, ... | 2004 | 20565598 |
| thrips resistance in pepper and its consequences for the acquisition and inoculation of tomato spotted wilt virus by the western flower thrips. | abstract different levels of thrips resistance were found in seven capsicum accessions. based on the level of feeding damage, host preference, and host suitability for reproduction, a thrips susceptible and a resistant accession were selected to study their performance as tomato spotted wilt virus (tswv) sources and targets during thrips-mediated virus transmission. vector resistance did not affect the virus acquisition efficiency in a broad range of acquisition access periods. inoculation effic ... | 2003 | 18944162 |
| restricted spread of tomato spotted wilt virus in thrips-resistant pepper. | abstract spread of tomato spotted wilt virus (tswv) and population development of its vector frankliniella occidentalis were studied on the pepper accessions cpro-1 and pikante reuzen, which are resistant and susceptible to thrips, respectively. viruliferous thrips were released on plants of each accession (nonchoice tests) or on plants in a 1:1 mixture of both accessions (choice tests) in small cages containing 8 or 16 plants. significantly fewer cpro-1 plants became infected in the primary inf ... | 2003 | 18944320 |
| negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. | posttranscriptional silencing of a green fluorescent protein (gfp) transgene in nicotiana benthamiana plants was suppressed when these plants were infected with tomato spotted wilt virus (tswv), a plant-infecting member of the bunyaviridae: infection with tswv resulted in complete reactivation of gfp expression, similar to the case for potato virus y, but distinct from that for cucumber mosaic virus, two viruses known to carry genes encoding silencing suppressor proteins. agrobacterium-based lea ... | 2003 | 12502849 |
| an unusual large intergenic region in the s-rna of a bulgarian tomato spotted wilt virus isolate. | the complete s-rna sequences of four bulgarian and one german isolate of tomato spotted wilt tospovirus were determined. all isolates show a high conservation in their n proteins, while the nss proteins and the intergenic regions (igr) are more variable. the bulgarian isolate 10hk96 has the largest s-rna (3364 nucleotides) among tomato spotted wilt viruses reported so far. the enlargement is based on an insertion of 365 nts in the igr that may have resulted from stuttering of the viral polymeras ... | 2003 | 12536305 |
| seasonal dispersal patterns of frankliniella fusca (thysanoptera: thripidae) and tomato spotted wilt virus occurrence in central and eastern north carolina. | the seasonal abundance and temporal pattern of frankliniella fusca hinds dispersal were monitored from 1996 to 2000 at 12 locations in central and eastern north carolina. the predominant vector species of tomato spotted wilt virus (tswv) captured across all locations was f. fusca (98%). the temporal patterns of f. fusca dispersal observed during spring seasons varied among locations in all years except 2000. regression analysis estimated that times of first flight in the spring seasons varied am ... | 2003 | 12650337 |
| detection of tomato spotted wilt virus in its vector frankliniella occidentalis by reverse transcription-polymerase chain reaction. | a method for rapid and reliable detection of tomato spotted wilt virus (tswv) (tospovirus, bunyaviridae) in its vector frankliniella occidentalis (thysanoptera thripidae) would be a useful tool for studying the epidemiology of this virus. a rt-pcr method developed for this purpose is reported. the method was tested on thrips involved in laboratory transmission trials and on thrips collected in the field, whose capability to transmit tswv was checked previously by leaf disk assays. the rt-pcr res ... | 2003 | 12668270 |
| peptide-mediated broad-spectrum plant resistance to tospoviruses. | plant viruses have a significant impact on agronomic losses worldwide. a new strategy for engineering virus-resistant plants by transgenic expression of a dominant interfering peptide is presented here. this peptide of 29 aa strongly interacts with the nucleocapsid proteins (n) of different tospoviruses. transgenic nicotiana benthamiana lines expressing the peptide fused to a carrier protein were challenged with five different tospoviruses that have a nucleocapsid protein interacting with the pe ... | 2003 | 12682295 |
| epidemiology and management of tomato spotted wilt in peanut. | tomato spotted wilt caused by thrips-vectored tomato spotted wilt virus (tswv) is a very serious problem in peanut (arachis hypogaea l.) production. tswv and the thrips frankliniella fusca and frankliniella occidentalis, which vector the virus, present a difficult and complicated challenge from the perspectives of both epidemiology and disease management. simply controlling the vector typically has not resulted in control of spotted wilt. no single measure can currently provide adequate control ... | 2003 | 12704217 |
| characterisation of the feeding behaviour of western flower thrips in terms of electrical penetration graph (epg) waveforms. | western flower thrips, frankliniella occidentalis (pergande) causes damage to plants when they are feeding. also, this thrips species transmits tomato spotted wilt virus (tswv) during stylet penetration. we investigated the penetration behaviour (probing) of thrips on pepper leaves and on liquid diet by electrical penetration graph (epg, dc-system) recording. in addition, we used high-magnification video observations to correlate epg waveforms with the insect's posture, head movements, and muscl ... | 2003 | 12769993 |
| association of l protein and in vitro tomato spotted wilt virus rna-dependent rna polymerase activity. | we have previously described an in vitro assay for rna-dependent rna polymerase activity in virions of tomato spotted wilt virus (tswv). here we report antibody inhibition of virion-associated rna synthesis in vitro with an l-protein-specific polyclonal antibody raised against the carboxy-terminus of the l protein. in contrast, rna synthesis was not inhibited by a heterologous antiserum and was unaffected by antiserum raised against an internal portion of the l protein. our results directly asso ... | 2003 | 12867756 |
| [changes in ultrastructure and protein metabolism of tobacco plants infected by tomato spotted wilt virus]. | as the result of electron microscope investigation of ultra-thin sections of the tissues infected by tomato spotted wilt virus it was shown that ultrastructural changes in the cells depend on the virus virulence. the isolate with low virulence induces mostly virus-specific changes (virus particles and virus inclusion bodies); the isolate with high virulence besides the virus-specific changes causes essential non-specific violation of cell organelle structure that could be the consequence of path ... | 2003 | 12945179 |
| integrated management tactics for frankliniella thrips (thysanoptera: thripidae) in field-grown pepper. | in a 2-yr study, the impacts of different plastic soil mulches, insecticides, and predator releases on frankliniella thrips and their natural enemies were investigated in field-grown peppers. ultraviolet light (uv)-reflective mulch significantly reduced early season abundance of adult thrips compared with standard black plastic mulch. this difference diminished as the growing seasons progressed. late season abundance of thrips larvae was higher in uv reflective mulch compared with black mulch pl ... | 2003 | 14503592 |
| selection of tomato plants resistant to a local polish isolate of tomato spotted wilt virus (tswv). | we found that the sw-5 gene confers resistance to one of the polish isolates of tomato spotted wilt virus (tswv). a series of tomato breeding accessions was analysed along with standards of resistance and susceptibility to tswv. the presence of the sw-5 gene was determined using the available pcr marker. subsequently plants from these accessions were grown in the presence of the tswv isolate from poland. some of them developed severe symptoms of the tswv disease. expression of the virus proteins ... | 2003 | 14617826 |
| factors determining vector competence and specificity for transmission of tomato spotted wilt virus. | the competence of a frankliniella occidentalis and a thrips tabaci population to transmit tomato spotted wilt virus (tswv) was analysed. adults of the f. occidentalis population transmitted this virus efficiently, whereas those of the thelytokous t. tabaci population failed to transmit. tswv replicated in the midgut of the larvae of both populations after ingestion of virus; however, lower amounts accumulated in t. tabaci larvae than in f. occidentalis larvae. the virus was almost undetectable i ... | 2002 | 11842261 |
| the detection of tomato spotted wilt virus (tswv) in individual thrips using real time fluorescent rt-pcr (taqman). | tomato spotted wilt virus (tswv) is an important virus, economically in the uk, causing damaging disease in ornamental and vegetable crops. the virus is vectored by several species of thrips, most importantly the western flower thrips (frankliniella occidentalis pergande [thysanoptera: thripidae]). the vector thrips themselves constitute a damaging pest and are difficult to control completely. monitoring thrips numbers is an important part of the control of virus, but does not give information o ... | 2002 | 11849682 |
| expression of the movement protein of tomato spotted wilt virus in its insect vector frankliniella occidentalis. | tomato spotted wilt virus (tswv) is able to infect both its botanical hosts and its insect vector (thrips). in plant tissue the ns(m) protein of tswv functions as viral movement protein (mp), aggregating into plasmodesma-penetrating tubules to establish cell-to-cell movement. as upon heterologous expression ns(m) was able to form similar tubules on the surface of insect (spodoptera frugiperda) cells, we have now investigated the expression and cellular manifestation of this protein in infected t ... | 2002 | 12038691 |
| induction of phenylpropanoid metabolism by pseudomonas fluorescens against tomato spotted wilt virus in tomato. | pseudomonas fluorescens (two native strains, one collection strain and their strain mixtures in all possible combinations) when applied through seed, seedling dip, soil and on leaf significantly reduced the tomato spotted wilt virus (tswv) disease. in p. fluorescens-treated plants, the peroxidase and phenylalanine ammonia-lyase activity increased. accumulation of phenolic compounds and lignin were shown to be increased in the p. fluorescens-treated plants. isoperoxidase native page indicated tha ... | 2002 | 12058389 |
| evaluation of metabolic detoxifying enzyme activities and insecticide resistance in frankliniella occidentalis. | the western flower thrips frankliniella occidentalis (pergande) is a very significant pest of a number of different agricultural crops in the south-east of spain. the importance of thrips as a pest is not due mainly to the direct damage inflicted on the plant, but to the loss in commercial value which occurs as a consequence of the development of dark spots caused by the tomato spotted wilt virus (tswv) which they transmit. the economic threshold is therefore almost zero, which enhances the prob ... | 2002 | 12233183 |
| the route of tomato spotted wilt virus inside the thrips body in relation to transmission efficiency. | the route of tomato spotted wilt virus (tswv) in the body of its vectors, frankliniella occidentalis and thrips tabaci (thysanoptera: thripidae) was studied during their development. first instar larvae were allowed, immediately upon hatching, to acquire virus from mechanically infected datura stramonium plants for 24 h. the rate of transmission by adults was determined in inoculation access feeding test on emilia sonchifolia leaf disks. thrips tissues were analysed for infection at 24 h interva ... | 2002 | 12417949 |
| identification of a novel rna silencing suppressor, nss protein of tomato spotted wilt virus. | rna silencing or post-transcriptional gene silencing (ptgs) in plants is known as a defense system against virus infection. several plant viruses have been shown to encode an rna silencing suppressor. using a green fluorescent protein-based transient suppression assay, we show that nss protein of tomato spotted wilt virus (tswv) has rna silencing suppressor activity. tswv nss protein suppressed sense transgene-induced ptgs but did not suppress inverted repeat transgene-induced ptgs. tswv nss pro ... | 2002 | 12459466 |
| purified tomato spotted wilt virus particles support both genome replication and transcription in vitro. | purified tomato spotted wilt virus particles were shown to support either genome replication or transcription in vitro, depending on the conditions chosen. transcriptional activity was observed only upon addition of rabbit reticulocyte lysate, indicating a dependence on translation. under these conditions rna molecules of subgenomic length were synthesized that hybridized to strand-specific probes for the n and nss genes. cloning of these transcripts demonstrated the presence of nonviral leader ... | 2002 | 12490389 |
| the efficiency by which thrips tabaci populations transmit tomato spotted wilt virus depends on their host preference and reproductive strategy. | abstract arrhenotokous and thelytokous populations of thrips tabaci from tobacco or leek plants were evaluated for their ability to transmit tomato spotted wilt virus (tswv) and for their host preference. transmission efficiencies were comparatively studied using leaf disks of petunia hybrida, datura stramonium, and nicotiana tabacum cv. basmas. adults of arrhenotokous populations collected on infected tobacco plants in the field were efficient transmitters (up to 48.5% transmission) and remaine ... | 2002 | 18944256 |
| dynamics of tomato spotted wilt virus replication in the alimentary canal of two thrips species. | abstract transmission of tomato spotted wilt virus (tswv) is dependent on virus uptake in the midgut prior to virus movement to the salivary glands. replication of tswv in the alimentary canal of tobacco thrips (tt, frankliniella fusca) and western flower thrips (wft, f. occidentalis) was investigated by immunolocalization of the nonstructural protein (nss) encoded by the small rna of tswv and fluorescence microscopy. analysis of cohorts during development from larva to adults following virus ac ... | 2002 | 18943268 |
| cross-talk between jasmonate and salicylate plant defense pathways: effects on several plant parasites. | plants are often attacked by many herbivorous insects and pathogens at the same time. two important suites of responses to attack are mediated by plant hormones, jasmonate and salicylate, which independently provide resistance to herbivorous insects and pathogens, respectively. several lines of evidence suggest that there is negative cross-talk between the jasmonate and salicylate response pathways. this biochemical link between general plant defense strategies means that deploying defenses agai ... | 2002 | 28547690 |
| replication of tomato spotted wilt virus after ingestion by adult thrips setosus is restricted to midgut epithelial cells. | abstract if acquisition access feeding (aaf) is first given after adult eclosion, none of the nine thrips species able to serve as tospovirus vectors can become infective. the previous cellular investigations of this phenomenon, carried out only in frankliniella occidentalis, suggested that infectivity was prevented because the type member of the tospoviruses, tomato spotted wilt virus (tswv), was unable to enter the midgut of adult thrips. the present study extends a cellular view of tospovirus ... | 2001 | 18943329 |
| completion of the genome sequence of watermelon silver mottle virus and utilization of degenerate primers for detecting tospoviruses in five serogroups. | abstract the nucleotide sequence of the l rna of watermelon silver mottle virus (wsmov) was determined. combined with the previous work on m and s rnas, the whole genomic sequence of this member of the genus tospovirus was completed. the l rna is 8,917 nucleotides in length, with one large open reading frame encoding a translation product of 2,878 amino acids (331.8 kda) on the viral complementary strand. the l protein shares amino acid identities of only 44.3 and 46.5% with tomato spotted wilt ... | 2001 | 18943848 |
| serological and molecular characterization of peanut chlorotic fan-spot virus, a new species of the genus tospovirus. | abstract to clarify the serological relationship of peanut chlorotic fan-spot virus (pcfv) with other tospoviruses, antisera were produced against the nucleocapsid (n) proteins of this virus and tospoviruses from four serogroups including tomato spotted wilt virus (tswv), impatiens necrotic spot virus (insv), groundnut ringspot virus (grsv), and watermelon silver mottle virus (wsmov). in immunodiffusion tests, the antisera only reacted with their homologous antigens. similar results were noticed ... | 2001 | 18944231 |
| overwintering of frankliniella fusca (thysanoptera: thripidae) on winter annual weeds infected with tomato spotted wilt virus and patterns of virus movement between susceptible weed hosts. | abstract overwintering of tobacco thrips, frankliniella fusca, was investigated on common winter annual host plants infected with tomato spotted wilt virus (tswv). populations of tobacco thrips produced on tswv-infected plants did not differ from those produced on healthy plants, whereas populations varied greatly among host plant species. the mean per plant populations of f. fusca averaged 401, 162, and 10 thrips per plant on stellaria media, scleranthus annuus, and sonchus asper, respectively, ... | 2001 | 18944235 |
| tomato spotted wilt virus glycoproteins exhibit trafficking and localization signals that are functional in mammalian cells. | the glycoprotein precursor (g1/g2) gene of tomato spotted wilt virus (tswv) was expressed in bhk cells using the semliki forest virus expression system. the results reveal that in this cell system, the precursor is efficiently cleaved and the resulting g1 and g2 glycoproteins are transported from the endoplasmic reticulum (er) to the golgi complex, where they are retained, a process that could be blocked by tunicamycin. expression of g2 alone resulted in transport to and retention in the golgi c ... | 2001 | 11134314 |
| gst fusion proteins cause false positives during selection of viral movement protein specific single chain antibodies. | glutathione s-transferase (gst) fusion proteins are used frequently for investigating protein-protein and protein-dna interactions. the present study demonstrates that the use of gst fusion proteins caused false positives during selection of phage-displayed single-chain antibody fragments (scfvs) specific for three domains of the movement protein (ns(m)) of tomato spotted wilt virus (tswv). to identify and exclude the false positives when using gst as a fusion partner linked to the antigen of in ... | 2001 | 11164495 |
| overcoming host- and pathogen-mediated resistance in tomato and tobacco maps to the m rna of tomato spotted wilt virus. | a viral genetic system was used to map the determinants of the ability of tomato spotted wilt virus (tswv) to overcome the r gene (sw-5) in tomato and the resistance conferred by the nucleocapsid gene of tswv (n gene) in tobacco. a complete set of reassortant genotypes was generated from tswv isolates a and d. tswv-a was able to overcome the sw-5 gene in tomato and the tswv n gene in tobacco, whereas tswv-d was repressed by both forms of resistance. the ability to overcome both forms of resistan ... | 2001 | 11204788 |
| variability of the n-protein and the intergenic region of the s rna of tomato spotted wilt tospovirus (tswv). | the genetic heterogeneity of the n protein gene and the intergenic region (igr) of the s rna from tomato spotted wilt virus (tswv) isolates, collected in bulgaria, were compared with isolates from other parts of the world. the results substantiated the highly conserved nature of the n protein. twenty six independent sequences revealed only seven variable amino acid positions, common to all isolates. the type of amino acids present in these positions seems to be independent of the geographical or ... | 2001 | 11346302 |
| in vivo analysis of the tswv cap-snatching mechanism: single base complementarity and primer length requirements. | requirements for capped leader sequences for use during transcription initiation by tomato spotted wilt virus (tswv) were tested using mutant alfalfa mosaic virus (amv) rnas as specific cap donors in transgenic nicotiana tabacum plants expressing the amv replicase proteins. using a series of amv rna3 mutants modified in either the 5'-non-translated region or in the subgenomic rna4 leader, sequence analysis revealed that cleaved leader lengths could vary between 13 and 18 nucleotides. cleavage oc ... | 2001 | 11350944 |
| rt-pcr for detecting five distinct tospovirus species using degenerate primers and dsrna template. | rt-pcr procedures for detection of multiple species of tospovirus from plant tissues were investigated. downstream primers were designated from the 3' untranslated sequences of the s rna. an upstream primer was designated from the degenerated sequences of the nucleocapsid protein. approximately 450 bp dna fragments were detected when tomato spotted wilt virus (tswv)- or impatiens necrotic spot virus (insv)- infected tissues were examined. approximately 350 bp dna fragments were detected when wat ... | 2001 | 11445145 |
| visual representation by atomic force microscopy (afm) of tomato spotted wilt virus ribonucleoproteins. | atomic force microscopy (afm) allows the observation of biological material without fixation procedures. here we present afm images of ribonucleoproteins (nucleocapsids) derived from a plant infecting rna virus (tomato spotted wilt virus, tswv), which have been recorded in contact mode. the nucleocapsids, prepared from systemically infected leaves of tobacco, were spreaded on a glass surface and dried in air, and appeared as regularly formed rings, resembling the proposed pseudocircular and panh ... | 2001 | 11767945 |
| constitutive expression of interferon-induced human mxa protein in transgenic tobacco plants does not confer resistance to a variety of rna viruses. | mxa is a key component in the interferon-induced antiviral defense in humans. after viral infections, mxa is rapidly induced and accumulates in the cytoplasm. the multiplication of many rna viruses, including all bunyaviruses tested so far, is inhibited by mxa. these findings prompted us to express mxa in plants in an attempt to create resistance to tospoviruses. here, we report the generation of transgenic tobacco plants that constitutively express mxa under the control of the 35 s cauliflower ... | 2000 | 11206971 |
| a minimum length of n gene sequence in transgenic plants is required for rna-mediated tospovirus resistance. | we showed previously that transgenic plants with the green fluorescent protein (gfp) gene fused to segments of the nucleocapsid (n) gene of tomato spotted wilt virus (tswv) displayed post-transcriptional gene silencing of the gfp and n gene segments and resistance to tswv. these results suggested that a chimeric transgene composed of viral gene segments might confer multiple virus resistance in transgenic plants. to test this hypothesis and to determine the minimum length of the n gene that coul ... | 2000 | 10640563 |
| a caps marker to assist selection of tomato spotted wilt virus (tswv) resistance in pepper. | the hypersensitive resistance to tomato spotted wilt virus (tswv) in pepper is determined by a single dominant gene (resistant allele: tsw) in several capsicum chinense genotypes. in order to facilitate the selection for this resistance, four rapd (among 250 10-mer primers tested) were found linked to the tsw locus using the bulked segregant analysis and 153 f2 individuals. a close rapd marker was converted into a codominant cleaved amplified polymorphic sequence (caps) using specific pcr primer ... | 2000 | 10701122 |
| simplified, rapid method for cloning of virus-binding polypeptides (putative receptors) via the far-western screening of a cdna expression library using purified virus particles. | a simplified, alternative method for cloning virus-binding polypeptides (receptor candidates) is described. the method is based on a far-western assay using purified tomato spotted wilt tospovirus (tswv, bunyaviridae) for screening a lambda-phage cdna expression library. the western flower thrips, frankliniella occidentalis pergande, the principal vector of tswv, in which the virus replicates, was used for library construction. using this method several virus-binding polypeptides were identified ... | 2000 | 10785290 |
| genetic mapping of the tsw locus for resistance to the tospovirus tomato spotted wilt virus in capsicum spp. and its relationship to the sw-5 gene for resistance to the same pathogen in tomato. | the tsw gene conferring dominant resistance to the tospovirus tomato spotted wilt virus (tswv) in capsicum spp. has been tagged with a random amplified polymorphic dna marker and mapped to the distal portion of chromosome 10. no mapped homologues of sw-5, a phenotypically similar dominant tswv resistance gene in tomato, map to this region in c. annuum, although a number of sw-5 homologues are found at corresponding positions in pepper and tomato. the relationship between tsw and sw-5 was also ex ... | 2000 | 10830267 |
| comparative genetics of disease resistance within the solanaceae. | genomic positions of phenotypically defined disease resistance genes (r genes) and r gene homologues were analyzed in three solanaceous crop genera, lycopersicon (tomato), solanum (potato), and capsicum (pepper). r genes occurred at corresponding positions in two or more genomes more frequently than expected by chance; however, in only two cases, both involving phytophthora spp., did genes at corresponding positions have specificity for closely related pathogen taxa. in contrast, resistances to ... | 2000 | 10835406 |
| immunoprecipitation of a 50-kda protein: a candidate receptor component for tomato spotted wilt tospovirus (bunyaviridae) in its main vector, frankliniella occidentalis. | a 50-kda protein that binds to viral particles in solid-phase assays and that is recognized by anti-idiotypic antibodies made against anti-viral glycoproteins g1/g2 (anti-ids) has been proposed as a receptor candidate for tomato spotted wilt tospovirus (tswv) in its main thrips vector, frankliniella occidentalis pergande (bandla et al., 1998. phytopathology 88, 98-104). here we show the immunoprecipitation of the 50-kda protein by anti-ids and by an anti-g1/g2-tswv conjugate - a new immunoprecip ... | 2000 | 10867190 |
| a single chimeric transgene derived from two distinct viruses confers multi-virus resistance in transgenic plants through homology-dependent gene silencing. | we showed previously that 218 and 110 bp n gene segments of tomato spotted wilt virus (tswv) that were fused to the non-target green fluorescent protein (gfp) gene were able to confer resistance to tswv via post-transcriptional gene silencing (ptgs). n gene segments expressed alone did not confer resistance. apparently, the gfp dna induced ptgs that targetted n gene segments and the incoming homologous tswv for degradation, resulting in a resistant phenotype. these observations suggested that mu ... | 2000 | 10900050 |
| real-time rt-pcr fluorescent detection of tomato spotted wilt virus. | a real-time reverse transcription-polymerase chain reaction assay based on taqman chemistry was developed for the detection and quantification of tomato spotted wilt virus (tswv). this method enabled sensitive, reproducible and specific detection of tswv in 'leaf soak' and total rna extracts from infected plants. taqman reliably detected tswv in as little as 500 fg total rna. the assay was 10-fold more sensitive than visualisation of ethidium bromide-stained bands following agarose gel electroph ... | 2000 | 10921836 |
| impeded thrips transmission of defective tomato spotted wilt virus isolates. | two defective rna-containing isolates (pe-1 and 16-2) and an envelope-deficient (env ) isolate of tomato spotted wilt virus (tswv) were tested for their transmissibility by frankliniella occidentalis. the pe-1 isolate contained a truncated l rna segment that barely interfered with symptom expression and replication of the wild-type (wt) l rna segment. this isolate was transmitted with an efficiency of 51%, a value comparable to that found for wt tswv (54%). isolate 16-2, which contained a genuin ... | 2000 | 18944549 |
| nucleocapsid gene-mediated transgenic resistance provides protection against tomato spotted wilt virus epidemics in the field. | abstract transformation of plants with the nucleocapsid (n) gene of tomato spotted wilt tospovirus (tswv) provides resistance to disease development; however, information is lacking on the response of plants to natural inoculum in the field. three tobacco cultivars were transformed with the n gene of a dahlia isolate of tswv (tswv-d), and plants were evaluated over several generations in the greenhouse. the resistant phenotype was more frequently observed in 'burley 21' than in 'ky-14' or 'k-326 ... | 2000 | 18944602 |
| application of phage display in selecting tomato spotted wilt virus-specific single-chain antibodies (scfvs) for sensitive diagnosis in elisa. | abstract a panel of recombinant single-chain antibodies (scfvs) against structural proteins of tomato spotted wilt virus (tswv) was retrieved from a human combinatorial scfv antibody library using the novel phage display technique. after subcloning the encoding dna sequences in the expression vector pskap/s, which allowed the scfvs to be expressed as alkaline phosphatase fusion proteins, 17 different scfv antibodies were obtained. of these, 12 scfvs were directed against the nucleoprotein (n) an ... | 2000 | 18944607 |
| tomato spotted wilt virus-positive steps towards negative success. | abstract taxonomy: tomato spotted wilt virus (tswv) is the type member of the plant-infecting tospovirus genus in the family bunyaviridae, a large group of predominantly vertebrate- and insect-infecting rna viruses. physical properties: virions are 80-120-nm pleomorphic particles with surface projections composed of two viral glycoproteins, g1 and g2 (fig. 1). virion composition is 5% nucleic acid, 70% protein, 5% carbohydrate and 20% lipid. the genome consists of three negative or ambisense ssr ... | 2000 | 20572961 |
| increase of tospoviral diversity in brazil with the identification of two new tospovirus species, one from chrysanthemum and one from zucchini. | abstract during a survey conducted in several different regions of brazil, two unique tospoviruses were isolated and characterized, one from chrysanthemum and the other from zucchini. the chrysanthemum virus displayed a broad host range, whereas the virus from zucchini was restricted mainly to the family cucurbitaceae. double-antibody sandwich-enzyme-linked immunosorbent assay and western immunoblot analyses demonstrated that both viruses were serologically distinct from all reported tospovirus ... | 1999 | 18944712 |
| tomato spotted wilt virus particle morphogenesis in plant cells. | a model for the maturation of tomato spotted wilt virus (tswv) particles is proposed, mainly based on results with a protoplast infection system, in which the chronology of different maturation events could be determined. by using specific monoclonal and polyclonal antisera in immunofluorescence and electron microscopy, the site of tswv particle morphogenesis was determined to be the golgi system. the viral glycoproteins g1 and g2 accumulate in the golgi prior to a process of wrapping, by which ... | 1999 | 9971812 |
| tissue tropism related to vector competence of frankliniella occidentalis for tomato spotted wilt tospovirus. | the development of tomato spotted wilt tospovirus (tswv) infection in the midgut and salivary glands of transmitting and non-transmitting thrips, frankliniella occidentalis, was studied to elucidate tissue tropism and the virus pathway within the body of this vector. immunohistological techniques used in this study showed that the midgut, foregut and salivary glands were the only organs in which virus accumulated. the first signals of infection, observed as randomly distributed fluorescent granu ... | 1999 | 10073714 |
| functional expression in bacteria and plants of an scfv antibody fragment against tospoviruses. | recombinant antibodies expressed in plants ('plantibodies'), directed against crucial antigens and addressed to the right cell compartment, may be able to protect against viral diseases. moreover, antibody fragments produced in bacteria or plants may provide low cost reagents for immunodiagnosis. | 1999 | 10231089 |
| alfalfa mosaic virus rnas serve as cap donors for tomato spotted wilt virus transcription during coinfection of nicotiana benthamiana. | tomato spotted wilt virus (tswv) was shown to use alfalfa mosaic virus (amv) rnas as cap donors in vivo during a mixed infection in nicotiana benthamiana. by use of nested reverse transcription-pcr, tswv n and nss mrnas provided with capped leader sequences derived from all four amv rnas could be cloned and sequenced. the sequence specificity of the putative tswv endonuclease involved is discussed. | 1999 | 10233983 |
| tomato spotted wilt virus in ornamental plants, vegetables and weeds in the czech republic. | the occurrence of tomato spotted wilt virus (tswv) in horticulture crops and weeds in the czech republic has been studied in 1992-1997. during this period tswv was established in 91 plant species. virus identity was based on the host range, serology and electron microscopy. natural tswv infection was detected in glasshouses where the main vector frankliniella occidentalis was present too. the most frequently tswv-infected plant species were chrysanthemum morifolium and zantedeschia sp. among veg ... | 1998 | 10358737 |
| an antiviral protein from bougainvillea spectabilis roots; purification and characterisation. | an antiviral protein active against mechanical transmission of tomato spotted wilt virus was identified in the root tissues of bougainvillea spectabilis willd. bougainvillea antiviral protein i (bap i) was purified to apparent homogeneity from the roots of bougainvillea by ammonium sulphate precipitation, cm- and deae-sepharose chromatography and reverse phase hplc. bap i is a highly basic protein (pi value > 8.6) with an mr of 28,000. the n-terminal sequence of bap i showed homology with other ... | 1998 | 9612957 |
| early events of tomato spotted wilt transcription and replication in protoplasts. | a protoplast transfection system for tomato spotted wilt tospovirus (tswv) was established by peg-mediated infection of tobacco protoplasts. analysis of viral rna synthesis revealed an asymmetric production of viral (v) and viral-complementary (vc) strands of all three genomic rna segments and a separation in time of subgenomic transcription during the first 72 hr post infection (p.i.). synthesis of vc-rna was detectable 8-10 hrs prior to v-rna production. during the first 72 h of infection the ... | 1998 | 9699207 |
| characterization of the nucleic acid binding properties of tomato spotted wilt virus nucleocapsid protein. | tomato spotted wilt tospovirus (tswv) is the type member of the plant-infecting viruses of the genus tospovirus in the family bunyaviridae. the three tswv rnas are encapsidated with nucleocapsid (n) protein to form ribonucleoprotein (rnp) which serves as the template for viral transcription and replication. regions of the open reading frame coding for the n protein on the small (s) rna were subcloned into pet protein expression vectors and expressed in escherichia coli bl21 (de3) cells. full-len ... | 1998 | 9705250 |
| molecular characterization of tomato spotted wilt virus defective interfering rnas and detection of truncated l proteins. | junction sites of 25 different defective interfering (di) rnas of tomato spotted wilt virus (tswv) were characterized. the di rnas varied in size from 2.0 to 5.2 kilobases (kb) and contained a single internal deletion. the absence of di rnas smaller than 2 kb suggested a size constraint for the survival of tswv di rnas. this hypothesis was reinforced by the finding of a dimeric di rna formed by two 1.6-long monomers linked head to tail. three types of junction sites were found, one type originat ... | 1998 | 9721243 |
| characterization of the large (l) rna of peanut bud necrosis tospovirus. | the nucleocapsids purified from peanut plants systemically infected with peanut bud necrosis virus (pbnv), a member of the genus tospovirus, contained both viral(v) and viral complementary(vc) sense l rnas. defective forms of l rna containing 'core polymerase region' were observed. the full length l rna of pbnv was sequenced using overlapping cdna clones. the 8911 nucleotide l rna contains a single open reading frame (orf) in the vc strand, and encodes a protein of 330 kda. at the 5' and 3' term ... | 1998 | 9930194 |
| comparison of ambisense m rna of watermelon silver mottle virus with other tospoviruses. | abstract double-stranded genomic rnas (dsrnas) extracted from chenopodium quinoa infected with watermelon silver mottle virus (wsmv) were similar to those of tomato spotted wilt virus (tswv, serogroup i) and impatiens necrotic spot virus (insv, serogroup iii), except that the s dsrna of wsmv is 0.75 and 0.6 kbp longer than those of tswv and insv, respectively. the complete nucleotide sequence of the genomic m rna of wsmv was determined from cdna clones generated from separated m dsrna. the m rna ... | 1998 | 18944959 |
| interaction of tomato spotted wilt tospovirus (tswv) glycoproteins with a thrips midgut protein, a potential cellular receptor for tswv. | abstract interactions between viral and cellular membrane fusion proteins mediate virus penetration of cells for many arthropod-borne viruses. electron microscope observations and circumstantial evidence indicate insect acquisition of tomato spotted wilt virus (tswv) (genus tospovirus, family bunyaviridae) is receptor mediated, and tswv membrane glycoproteins (gp1 and gp2) serve as virus attachment proteins. the tospoviruses are plant-infecting members of the family bunyaviridae and are transmit ... | 1998 | 18944977 |
| binding of tomato spotted wilt virus to a 94-kda thrips protein. | abstract using protein blot assays, a 94-kda thrips protein was identified that exhibited specific binding to tomato spotted wilt virus (tswv) particles. renaturation of the 94-kda protein, which is conserved among the two major vector species of tswv, frankliniella occidentalis and thrips tabaci, was crucial for its virus-binding properties, whereas under the same conditions no specific binding was observed with aphid (myzus persicae) proteins. the 94-kda protein species was present in all deve ... | 1998 | 18945001 |
| effects of temperature and host on the generation of tomato spotted wilt virus defective interfering rnas. | abstract the generation of defective interfering (di) rna molecules of tomato spotted wilt tospovirus (tswv) was studied by serially passaging in-ocula from plant to plant under different controlled conditions. di rnas were generated at higher rates in plants at 16 degrees c than in plants incubated at higher temperatures. another factor promoting the tswv di rna generation was the use of high virus concentrations in the inocula. the solanaceous species capsicum annuum, datura stramonium, lycope ... | 1997 | 18945014 |
| characterization of rna-mediated resistance to tomato spotted wilt virus in transgenic tobacco plants expressing ns(m) gene sequences. | transgenic nicotiana tabacum plants expressing rna sequences of the tomato spotted wilt virus ns(m) gene, which encodes the putative viral movement protein, were found to be highly resistant to infection with the virus. expression of untranslatable as well as anti-sense rna of the ns(m) gene resulted in resistance levels as high as those in plants expressing translatable rna sequences. for all three types of transgenic plants resistance levels of up to 100% were reached in the s2 progeny. these ... | 1997 | 9037142 |
| serological comparison of tospoviruses with polyclonal antibodies produced against the main structural proteins of tomato spotted wilt virus. | a new purification procedure for the tospoviruses of serogroups ii and iii was developed. sds-polyacrylamide gel electrophoresis of purified tomato spotted wilt virus (tswv; serogroup i), groundnut ringspot virus (grsv: serogroup ii), tomato chlorotic spot virus (tcsv; serogroup ii) and impatiens necrotic spot virus (insv; serogroup iii) showed that the glycoprotein g2 of serogroup ii members differs significantly in size from that of the serogroup i virus. western immunoblot analysis using poly ... | 1997 | 9170504 |
| multiplication of tomato spotted wilt virus in primary cell cultures derived from two thrips species. | primary cell cultures prepared from embryos of the thrips species frankliniella occidentalis and thrips tabaci were tested for their potential to support replication of tomato spotted wilt virus (tswv). using polyclonal antibodies against the viral nucleocapsid protein (n) and indirect immunofluorescent staining, discrete spots with strong signals were observed in the cytoplasm at 48 h post-inoculation in the cell cultures of a f. occidentalis, and a t. tabaci population which failed to transmit ... | 1997 | 9178497 |
| nontarget dna sequences reduce the transgene length necessary for rna-mediated tospovirus resistance in transgenic plants. | rna-mediated virus resistance has recently been shown to be the result of post-transcriptional transgene silencing in transgenic plants. this study was undertaken to characterize the effect of transgene length and nontarget dna sequences on rna-mediated tospovirus resistance in transgenic plants. transgenic nicotiana benthamiana plants were generated to express different regions of the nucleocapsid (n) protein of tomato spotted wilt (tswv) tospovirus. transgenic plants expressing half-gene segme ... | 1997 | 9223349 |
| a protoplast system for studying tomato spotted wilt virus infection. | a plant protoplast system for studying tomato spotted wilt tospovirus (tswv) infection was established and tested. using polyethylene glycol-mediated inoculation with highly infectious tswv particles, generally 50% or more of nicotiana rustica protoplasts were infected. in these cells viral rna and viral protein synthesis became detectable at 16 h post-inoculation (p.i.) and continued at least until 90 h p.i. both the structural viral proteins [nucleoprotein (n) and the envelope glycoproteins g1 ... | 1997 | 9225052 |
| cladistic analysis of tospovirus using molecular characters. | the genus tospovirus was thought to be composed only of the tomato spotted wilt virus (tswv), but now at least four tospovirus species have been proposed based on serological and molecular data. a classification of tospoviruses has been proposed taking into account global similarities of the n gene and n protein sequences of 7 isolates of tospovirus. because phylogenetic analyses based on global similarities can lead to classifications which do not mirror the genealogy of the group, we have empl ... | 1997 | 9242593 |
| map-based cloning of the tomato genomic region that spans the sw-5 tospovirus resistance gene in tomato. | two yeast artificial chromosomes (yacs) containing genomic dna from tomato have been isolated using ct220, an rflp marker which is tightly linked to the tomato spotted wilt virus resistance gene, sw-5. high-resolution mapping of the yac ends and internal yac probes demonstrated that one of the yac clones, ty257 (400 kb), spans sw-5. by chromosome walking in a cosmid library, the position of sw-5 has been delimited within the yac to a maximal chromosomal segment of 100 kb, spanned by nine overlap ... | 1997 | 9349703 |
| sequence analysis of the 5' ends of tomato spotted wilt virus n mrnas. | messenger rnas transcribed from the tomato spotted wilt virus (tswv) rna genome have characteristic extra non-templated heterogeneous sequences at their 5' ends which may be the result of a cap-snatching event involving cellular mrnas. in order to investigate the genetic origin of these extra sequences and to gain more insight in the process of cap-snatching as performed by tswv, nucleocapsid protein (n) mrnas derived from the tswv s rna were cloned and sequenced. twenty clones were obtained whi ... | 1996 | 8629946 |
| engineered rna-mediated resistance to tomato spotted wilt virus is sequence specific. | transgenic plants were produced that expressed a wide range of randomly chosen sequences of the tripartite tomato spotted wilt virus (tswv) rna genome or its complement. testing the progenies of these plants revealed that only transgenic expression of n or ns(m) gene sequences resulted in resistance to tswv. | 1996 | 8672819 |
| the complete nucleotide sequence and genome organization of the m rna segment of peanut bud necrosis tospovirus and comparison with other tospoviruses. | the m rna of peanut bud necrosis virus (pbnv; synonym groundnut bud necrosis virus) is 4801 nucleotides in length. it comprised two orfs in an ambisense organization and terminal inverted repeats. the 3' large orf (3363 nucleotides in the virus-complementary strand) encoded a protein with a predicted size of 127.2 kda which was identified as the glycoprotein precursor (gp) of the g1 and g2 glycoproteins. a comparison of the deduced amino acid sequence of gp revealed 37% identity and 58-59% simil ... | 1996 | 8811036 |
| a polyclonal antiserum against a recombinant viral protein combines specificity with versatility. | a polyclonal rabbit antiserum was obtained to the nucleoprotein of tomato spotted wilt tospovirus expressed as a recombinant fusion protein in e. coli. in indirect plate trapping elisa, the antiserum gave similar titres against purified tswv nucleocapsids in native form, the fusion protein and the carrier protein. the crude antiserum was also tested by western blotting, indirect plate trapping elisa and immunogold electron microscopy of thin sections: purified immunoglobulins were tested by das- ... | 1996 | 8882651 |
| an improved method for the detection of tospoviruses using the polymerase chain reaction. | a reverse transcription-polymerase chain reaction (rt-pcr)-based assay for the detection of tomato spotted wilt virus (tswv) has been improved and extended to enable the detection of additional tospoviruses. in addition to tswv-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (insv) and another which detects all tospoviruses tested, including tswv, insv, tomato chlorotic spot virus and groundnut ringspot virus. a ... | 1996 | 8919828 |
| clustering of argentinean tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide rna region of the n gene. | the genomic diversity of argentine tospoviruses from different geographical areas, and from several distinct crops was analysed here. for each isolate, rt-pcr, cloning and sequencing of a 450 nt fragment of the n gene were performed. comparisons of rna and predicted amino acid sequence identity and similarity were made. a partial sequence of the n gene was able to classify our local isolates within three tospovirus species previously described (tomato spotted wilt virus, tswv; tomato chlorotic s ... | 1996 | 9035370 |
| identification and mapping on chromosome 9 of rapd markers linked to sw-5 in tomato by bulked segregant analysis. | bulked segregant analysis was used to identify random amplified polymorphic dna (rapd) markers linked to the sw-5 gene for resistance to tomato spotted wilt virus (tswv) in tomato. using two pools of phenotyped individuals from one segregating population, we identified four rapd markers linked to the gene of interest. two of these appeared tightly linked to sw-5, whereas another, linked in repulsion phase, enabled the identification of heterozygous and susceptible plants. after linkage analysis ... | 1996 | 24166634 |