Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
the severity of acute puumala hantavirus infection does not predict the long-term outcome of patients.we have found greater urinary protein excretion and higher glomerular filtration rate (gfr) and blood pressure in patients 6 years after acute nephropathia epidemica (ne) compared with seronegative controls. the present aim was to establish whether the long-term outcome is determined by the severity of acute illness.201020502044
steep rise in notified hantavirus infections in germany, april 2010.from january to april 2010, 396 hantavirus infections were notified in germany, a considerable increase compared with previous years (mean: 83 for january-april 2004-2009) including the record-setting year, 2007 (n=232 january-april). most patients are residents of known puumala virus endemic areas in southern germany. the recent increase in notified hantavirus infections is probably due to an increased population density of the main animal reservoir, the bank vole (myodes glareolus).201020504391
small mammal-associated zoonoses. 201020795915
enhanced thrombin formation and fibrinolysis during acute puumala hantavirus infection.nephropathia epidemica (ne) is a viral hemorrhagic fever with renal syndrome associated with thrombocytopenia and mild bleeding. we assessed activation of coagulation and fibrinolysis during the acute phase of ne.201020579693
[dynamics of hantavirus detection in the excretory organs of apodemus mice and its relation to epidemic manifestations of hantavirus infection].this paper presents data on the detection of hantavirus in the excretory organs of apodemus mice which are natural carriers of pathogenic hantaviral serotypes in the primorye region. acute infection periods were detected in naturally infected rodents with the highest hantavirus dissemination to the environment in different phases of rodent population cycles. there was a relationship between the rise of morbidity and the seasonal manifestation of hemorrhagic fever with renal syndrome morbidity an ...201020455471
models for the spread and persistence of hantavirus infection in rodents with direct and indirect transmission.hantavirus, a zoonotic disease carried by wild rodents, is spread among rodents via direct contact and indirectly via infected rodent excreta in the soil. spillover to humans is primarily via the indirect route through inhalation of aerosolized viral particles. rodent-hantavirus models that include direct and indirect transmission and periodically varying demographic and epidemiological parameters are studied in this investigation. two models are analyzed, a nonautonomous system of differential ...201020462286
sin nombre virus-specific immunoglobulin m and g kinetics in hantavirus pulmonary syndrome and the role played by serologic responses in predicting disease outcome.sin nombre virus (snv) is the primary cause of hantavirus pulmonary syndrome (hps) in the united states. although other studies have demonstrated a possible association between neutralizing antibody titers and the severity of hps, the exact nature of serologic responses and their association with outcomes have not been fully characterized.201020521946
convalescent pulmonary dysfunction following hantavirus pulmonary syndrome in panama and the united states.the objective of this study was to document persistent pulmonary symptoms and pulmonary function abnormalities in adults surviving hantavirus pulmonary syndrome (hps). acute infection by most hantaviruses result in mortality rates of 25-35%, while in panama the mortality rate of 10% is contrasted by an unusually high incidence. in all types of hps, the viral prodrome, cardiopulmonary phase due to massive pulmonary capillary leak syndrome, and spontaneous diuresis are followed by a convalescent p ...201020524006
the severity of puumala hantavirus induced nephropathia epidemica can be better evaluated using plasma interleukin-6 than c-reactive protein determinations.nephropathia epidemica (ne) is a scandinavian type of hemorrhagic fever with renal syndrome caused by puumala hantavirus. the clinical course of the disease varies greatly in severity. the aim of the present study was to evaluate whether plasma c-reactive protein (crp) and interleukin (il)-6 levels associate with the severity of ne.201020500875
ecology of hantavirus in a changing world.hantavirus is a genus of virus represented by 45 different species and is hosted by small mammals, predominantly rats and mice. roughly, half of all hantaviruses cause diseases in humans that vary in morbidity from mild to severe. the natural and anthropogenic changes occurring in the environment appear to be impacting the ecology of hantaviruses and their natural hosts as well as the incidence of hantaviral diseases in humans. although such studies are limited at this time, there is evidence th ...201020536819
tnf-α expression and promoter sequences reflect the balance of tolerance/resistance to puumala hantavirus infection in european bank vole populations.the tumor necrosis factor-alpha (tnf-α) influences the ability to limit parasite infection but its over-production might result in inflammatory disorders. the level of tnf-α gene expression could thus mediate a balance of tolerance/resistance to infections. this study focused on puumala hantavirus (puuv) infection in its rodent host, the bank vole (myodes glareolus). in humans, puuv is responsible of a mild form of hemorrhagic fever with renal syndrome, nephropathia epidemica (ne). the severity ...201020691810
central nervous system-related symptoms and findings are common in acute puumala hantavirus infection.puumala hantavirus (puuv) causes a hemorrhagic fever with renal syndrome (hfrs) also called nephropathia epidemica (ne). recent case reports and retrospective studies suggest that ne may damage the pituitary gland. based on these observations, our goal was to explore the nature of this complication prospectively.201020545485
[hantavirus infection associated to a staphylococcal sepsis. report of one case].we report a previously healthy 36-year-old mole who developed a pulmonary syndrome caused by hantavirus infection. he was admitted to an intensive care unit in shock and respiratory failure and died four hours after admission. blood cultures were positive for staphylococcus aureus. this patient could be an index case that shows, for the first time, an association of hantavirus pulmonary syndrome with staphylococcus aureus infection similar to what happens with other viruses.201020556338
travellers and viral haemorrhagic fevers: what are the risks?viral haemorrhagic fevers (vhf) are caused by zoonotic viral infections transmitted to humans directly or by ticks or mosquitoes. the overall risk to travellers is conservatively estimated at <1 in 1 million travel episodes to african countries where infection is present, and febrile patients returning from these countries are at least 1000 times more likely to have malaria than lassa fever or another vhf. no cases have been reported in fellow travellers exposed to a travelling case and only one ...201020705436
interaction between hantaviral nucleocapsid protein and the cytoplasmic tail of surface glycoprotein gn.hantaviral n and gn proteins were shown to interact, thus providing the long-awaited evidence for one of the crucial steps in the virus replication at which rnps are directed to the site of the virus assembly. using pull-down assay and point mutagenesis it was demonstrated that intact, properly folded zinc fingers in the gn protein cytoplasmic tail as well as the middle domain of the n protein (that includes aa residues 80-248) are essential for the interaction.201020566401
molecular characterization of two hantavirus strains from different rattus species in singapore.hantaviruses cause human disease in endemic regions around the world. outbreaks of hantaviral diseases have been associated with changes in rodent population density and adaptation to human settlements leading to their proliferation in close proximity to human dwellings. in a parallel study initiated to determine the prevalence of pathogens in singapore's wild rodent population, 1206 rodents were trapped and screened. the findings established a hantavirus seroprevalence of 34%. this paper descri ...201020096099
hantavirus transmission in sylvan and peridomestic environments.we developed a compartmental model for hantavirus infection in deer mice (peromyscus maniculatus) with the goal of comparing relative importance of direct and indirect transmission in sylvan and peridomestic environments. a direct transmission occurs when the infection is mediated by the contact of an infected and an uninfected mouse, while an indirect transmission occurs when the infection is mediated by the contact of an uninfected mouse with, for instance, infected soil. based on population d ...201019821001
hantavirus nephropathy as a pseudo-import pathology from ecuador.we report a case of hantavirus infection (nephropathia epidemica) diagnosed in a belgian backpacker returning from a trekking expedition in ecuador, after likely heavy exposure to rodents. because of epidemiological inconsistency, molecular investigation was performed and revealed a puumala infection acquired during very limited exposure in belgium upon return.201019821128
interactions and oligomerization of hantavirus glycoproteins.in this report the basis for the structural architecture of the envelope of hantaviruses, family bunyaviridae, is systematically studied by the interactions of two glycoproteins n and c (gn and gc, respectively) and their respective disulfide bridge-mediated homo- and heteromeric oligomerizations. in virion extracts gn and gc associated in both homo- and hetero-oligomers which were, at least partially, thiol bridge mediated. due to strong homo-oligomerization, the hetero-oligomers of gn and gc a ...201019828613
ecology of hantaviruses and their hosts in north america.since the 1993 discovery of a highly pathogenic hantavirus associated with the north american deer mouse (peromyscus maniculatus), intensive ecological studies have led to many advances in our understanding of the natural history of new world hantaviruses as it relates to human disease. seventeen named hantaviruses have been identified in north america. field and laboratory studies of sin nombre and other hantaviruses have delineated host associations, geographical distributions, mechanisms of t ...201019874190
extensive host sharing of central european tula virus.to examine the host association of tula virus (tulv), a hantavirus present in large parts of europe, we investigated a total of 791 rodents representing 469 microtus arvalis and 322 microtus agrestis animals from northeast, northwest, and southeast germany, including geographical regions with sympatric occurrence of both vole species, for the presence of tulv infections. based on serological investigation, reverse transcriptase pcr, and subsequent sequence analysis of partial small (s) and mediu ...201019889769
seoul virus suppresses nf-kappab-mediated inflammatory responses of antigen presenting cells from norway rats.hantavirus infection reduces antiviral defenses, increases regulatory responses, and causes persistent infection in rodent hosts. to address whether hantaviruses alter the maturation and functional activity of antigen presenting cells (apcs), rat bone marrow-derived dendritic cells (bmdcs) and macrophages (bmdms) were generated and infected with seoul virus (seov) or stimulated with tlr ligands. seov infected both dcs and macrophages, but copies of viral rna, viral antigen, and infectious virus ...201020170933
tula hantavirus nss protein accumulates in the perinuclear area in infected and transfected cells.the small rna segment of some hantaviruses (family bunyaviridae) encodes two proteins: the nucleocapsid protein and, in an overlapping reading frame, a non-structural (nss) protein. the hantavirus nss protein, like those of orthobunya- and phleboviruses, counteracts host innate immunity. here, for the first time, the nss protein of a hantavirus (tula virus) has been observed in infected cells and shown to localize in the perinuclear area. transiently expressed nss protein showed similar localiza ...201019956987
the effect of seasonality, density and climate on the population dynamics of montana deer mice, important reservoir hosts for sin nombre hantavirus.1. since sin nombre virus was discovered in the u.s. in 1993, longitudinal studies of the rodent reservoir host, the deer mouse (peromyscus maniculatus) have demonstrated a qualitative correlation among mouse population dynamics and risk of hantavirus pulmonary syndrome (hps) in humans, indicating the importance of understanding deer mouse population dynamics for evaluating risk of hps. 2. using capture-mark-recapture statistical methods on a 15-year data set from montana, we estimated deer mous ...201020015212
hantavirus pulmonary syndrome.hantavirus pulmonary syndrome, also known as hantavirus cardiopulmonary syndrome, is a recently described infectious syndrome found throughout the americas. although infection is sporadic and uncommon compared with other atypical pneumonia syndromes, its high mortality rate warrants the maintenance of a high index of suspicion in rural settings. because no specific therapies are available for the disease, prevention and early recognition play an important role in reducing mortality from the dise ...201020171551
highly differentiated, resting gn-specific memory cd8+ t cells persist years after infection by andes hantavirus.in man, infection with south american andes virus (andv) causes hantavirus cardiopulmonary syndrome (hcps). hcps due to andv is endemic in southern chile and much of argentina and increasing numbers of cases are reported all over south america. a case-fatality rate of about 36% together with the absence of successful antiviral therapies urge the development of a vaccine. although t-cell responses were shown to be critically involved in immunity to hantaviruses in mouse models, no data are availa ...201020174562
pathogenic hantaviruses direct the adherence of quiescent platelets to infected endothelial cells.hantavirus infections are noted for their ability to infect endothelial cells, cause acute thrombocytopenia, and trigger 2 vascular-permeability-based diseases. however, hantavirus infections are not lytic, and the mechanisms by which hantaviruses cause capillary permeability and thrombocytopenia are only partially understood. the role of beta(3) integrins in hemostasis and the inactivation of beta(3) integrin receptors by pathogenic hantaviruses suggest the involvement of hantaviruses in altere ...201020181715
serologic survey of hantavirus infection, brazilian amazon. 201020409399
hantaviruses in small mammals and humans in the coastal region of zhejiang province, china.to gain more insights into the epidemiology of hantaviruses in the coastal region of zhejiang province, china, the morbidity and mortality of hemorrhagic fever with renal syndrome (hfrs) were analyzed in two coastal areas: cixi (hilly terrain) and wenzhou (mountainous terrain). more hfrs cases have been reported in cixi than in wenzhou. annual incidence rate of hfrs in cixi had been on the level of approximately 1.5/100,000 from 1968 (when the first hfrs case was reported) to 2007, with the high ...201020419812
models for the spread and persistence of hantavirus infection in rodents with direct and indirect transmission.hantavirus, a zoonotic disease carried by wild rodents, is spread among rodents via direct contact and indirectly via infected rodent excreta in the soil. spillover to humans is primarily via the indirect route through inhalation of aerosolized viral particles. rodent-hantavirus models that include direct and indirect transmission and periodically varying demographic and epidemiological parameters are studied in this investigation. two models are analyzed, a nonautonomous system of differential ...201020104956
one-step rna pathogen detection with reverse transcriptase activity of a mutated thermostable thermus aquaticus dna polymerase.we describe the cloning and characterization of a mutated thermostable dna polymerase from thermus aquaticus (taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step pcr pathogen detection using established real-time detection methods. we demonstrate that this taq polymerase mutant (taq m1) has similar pcr sensitivity and nuclease activity as the respective taq wild-type dna polymerase. in addition, and in marked contrast to the wild-type, taq m1 e ...201020108275
seewis virus: phylogeography of a shrew-borne hantavirus in siberia, russia.hantaviral antigens were originally reported more than 20 years ago in tissues of the eurasian common shrew (sorex araneus), captured in european and siberian russia. the recent discovery of seewis virus (swsv) in this soricid species in switzerland provided an opportunity to investigate its genetic diversity and geographic distribution in russia.201020426688
sin nombre virus infection in field workers, colorado, usa.we report 2 cases of sin nombre virus (snv) infection in field workers, possibly contracted through rodent bites. screening for antibodies to snv in rodents trapped in 2 seasons showed that 9.77% were seropositive. quantitative real-time pcr showed that 2 of 79 deer mice had detectable titers of snv rna.201020113567
comprehensive panel of real-time taqman polymerase chain reaction assays for detection and absolute quantification of filoviruses, arenaviruses, and new world hantaviruses.viral hemorrhagic fever is caused by a diverse group of single-stranded, negative-sense or positive-sense rna viruses belonging to the families filoviridae (ebola and marburg), arenaviridae (lassa, junin, machupo, sabia, and guanarito), and bunyaviridae (hantavirus). disease characteristics in these families mark each with the potential to be used as a biological threat agent. because other diseases have similar clinical symptoms, specific laboratory diagnostic tests are necessary to provide the ...201020439981
different cross-reactivity of human and rodent sera to tula virus and puumala virus.tula virus (tulv) and puumala virus (puuv) are hantaviruses carried by the bank vole (myodes glareolus) and european common vole (microtus arvalis), respectively. puuv is a causative agent of hemorrhagic fever with renal syndrome (hfrs), while tulv is thought to be apathogenic to humans. the n-terminal regions of the n proteins from tulv and puuv were expressed and applied as enzyme-linked immunosorbent assay (elisa) antigens. colonized japanese grass voles (microtus montebelli) and balb/c mice ...201020116854
[hantavirosis: clinical and epidemiological characteristics of pediatric patients in chile].the first cases of hantavirus cardiopulmonary syndrome in children were described in the united states and represented 8% of the total reported cases; in chile, the proportion of pediatric cases represents 15% of all national cases.201020140316
hantavirus nucleocapsid protein has distinct m7g cap- and rna-binding sites.hantaviruses, members of the bunyaviridae family, are emerging category a pathogens that carry three negative stranded rna molecules as their genome. hantavirus nucleocapsid protein (n) is encoded by the smallest s segment genomic rna (viral rna). n specifically binds mrna caps and requires four nucleotides adjacent to the cap for high affinity binding. we show that the n peptide has distinct cap- and rna-binding sites that independently interact with mrna cap and viral genomic rna, respectively ...201020164193
pathology of black creek canal virus infection in juvenile hispid cotton rats (sigmodon hispidus).the purpose of this study was to assess the effect of inoculum dose on the pathogenesis of black creek canal virus (bccv) infection in the hispid cotton rat (sigmodon hispidus), the principal host of bccv. no sign of illness was observed in any of the 52 juvenile hispid cotton rats inoculated with 3.1, 1.1, -0.9, or -2.9 log(10) median infectious doses(veroe6) (id(50-veroe6)) of bccv and euthanized on day 9, 18, 27, or 54 postinoculation (pi). analysis of virus assay and serological data indicat ...201020455779
generation of e3-deleted canine adenovirus type 2 expressing the gc glycoprotein of seoul virus by gene insertion or deletion of related terminal region sequences.seoul virus (seov) is one of the four hantaviruses known to cause haemorrhagic fever with renal syndrome. the medium genome segment encodes the gn/gc glycoproteins of seov, which form the major structural part of the virus envelope. gc and/or gn are the candidate antigens of hantavirus for induction of a highly immunogenic response for hantavirus vaccine. in this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the gc protein of seov w ...201020181748
beechnuts and outbreaks of nephropathia epidemica (ne): of mast, mice and men. 201020237057
infection of in vivo differentiated human mast cells with hantaviruses.increased vascular permeability is a key feature of the pathological symptoms caused by hantaviruses. here, we analysed the interaction between hantaviruses and mast cells, which regulate vascular homeostasis. in highly purified human skin mast cells increasing amounts of hantaan (htnv) and, to a lower extent, prospect hill (phv) virions were produced. replication was confirmed by the production of viral plus-strand rna as determined by a virus strand-specific rt-pcr. phv but not htnv elicited e ...201020071485
epidemic viral pneumonia.two recent viral epidemics producing pneumonitis (severe acute respiratory syndrome and pandemic influenza a h1n1) have highlighted the potential for viral infections to cause respiratory failure with a significant risk of mortality. this review describes these epidemics and other causes of epidemic viral pneumonia.201020087179
association of -308g/a polymorphism in the tumor necrosis factor-alpha gene promoter with susceptibility to development of hantavirus cardiopulmonary syndrome in the ribeirão preto region, brazil.activation of the immune response in hantavirus cardiopulmonary syndrome (hcps) leads to a high tnf production, probably contributing to the disease. the polymorphic tnf2 allele (tnf -308g/a) has been associated with increased cytokine production. we investigated the association of the tnf2 allele with the outcome of hantavirus infection in brazilian patients. a total of 122 hantavirus-exposed individuals (26 presenting hcps and 96 only hantavirus seroconversion) were studied. the tnf2 allele wa ...201020372945
serological evidence of human hantavirus infections in guinea, west africa.we recently discovered a novel hantavirus, sangassou virus, in guinea, west africa. using enzyme-linked immunosorbent assays followed by confirmatory and serotyping assays, we retrospectively detected hantavirus antibodies in 3 (4.4%) of 68 patients with fever of unknown origin in sangassou village, forest guinea. a population-based survey in forest guinea (n = 649) found the prevalence of hantavirus antibodies to be 1.2%. specific neutralizing antibodies against sangassou virus were demonstrate ...201020187741
comparison of the effects of puumala and dobrava viruses on early and long-term renal outcomes in patients with haemorrhagic fever with renal syndrome.the clinical course and outcome of patients with haemorrhagic fever with renal syndrome (hfrs) caused by puumala (puuv) and dobrava viruses (dobv) were analyzed and whether it left long-term consequences on kidney function after 10 years was evaluated.201020470304
electron cryotomography of tula hantavirus suggests a unique assembly paradigm for enveloped viruses.hantaviruses (family bunyaviridae) are rodent-borne emerging viruses that cause a serious, worldwide threat to human health. hantavirus diseases include hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome. virions are enveloped and contain a tripartite single-stranded negative-sense rna genome. two types of glycoproteins, g(n) and g(c), are embedded in the viral membrane and form protrusions, or "spikes." the membrane encloses a ribonucleoprotein core, which consists of ...201020219926
characterization and outcome following puumala virus infection: a retrospective analysis of 75 cases.infection with the puumala virus (puuv), which belongs to the hantavirus family, is a common but often neglected cause of acute kidney injury (aki) in endemic areas of europe. the objective of the present study was to systematically analyse clinical presentation and renal outcomes following puuv infection.201020223893
truncated hantavirus nucleocapsid proteins for serotyping sin nombre, andes, and laguna negra hantavirus infections in humans and rodents.sin nombre virus (snv), andes virus (andv), and laguna negra virus (lanv) have been known as the dominant causative agents of hantavirus pulmonary syndrome (hps). andv and lanv, with different patterns of pathogenicity, exist in a sympatric relationship. moreover, there is documented evidence of person-to-person transmission of andv. therefore, it is important in clinical medicine and epidemiology to know the serotype of a hantavirus causing infection. truncated snv, andv, and lanv recombinant n ...201020335425
hantavirus pulmonary syndrome, french guiana. 201020350412
high-resolution computed tomography findings in hantavirus pulmonary syndrome.the hantavirus pulmonary syndrome (hps) is an acute, rapidly progressive disease transmitted by rodent excreta, with endothelial damage playing a central role in the pathophysiology. it usually affects rural workers. the lung itself is the target organ and reflects all the patterns of endothelial involvement of this disease. the radiologic findings of hps are vast and range from a mild interstitial involvement to total obliteration of the airspaces with or without pleural effusion. there are no ...201020351581
recognition of decay accelerating factor and alpha(v)beta(3) by inactivated hantaviruses: toward the development of high-throughput screening flow cytometry assays.hantaviruses cause two severe diseases in humans: hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps). the lack of vaccines or specific drugs to prevent or treat hfrs and hcps and the requirement for conducting experiments in a biosafety level 3 laboratory (bsl-3) limit the ability to probe the mechanism of infection and disease pathogenesis. in this study, we developed a generalizable spectroscopic assay to quantify saturable fluorophore sites solubilized ...201020363206
pathogenic hantaviruses andes virus and hantaan virus induce adherens junction disassembly by directing vascular endothelial cadherin internalization in human endothelial cells.hantaviruses infect endothelial cells and cause 2 vascular permeability-based diseases. pathogenic hantaviruses enhance the permeability of endothelial cells in response to vascular endothelial growth factor (vegf). however, the mechanism by which hantaviruses hyperpermeabilize endothelial cells has not been defined. the paracellular permeability of endothelial cells is uniquely determined by the homophilic assembly of vascular endothelial cadherin (ve-cadherin) within adherens junctions, which ...201020463083
a case report of crescentic glomerulonephritis associated with hantaan virus infection.although various glomerular diseases in hantavirus infection have been reported, an association between hantavirus infection and crescentic glomerulonephritis has not been described. herein, we report a case of immune complex-mediated crescentic glomerulonephritis in a 70-year-old man with hantaan virus infection.201020466689
the triplet repeats of the sin nombre hantavirus 5' untranslated region are sufficient in cis for nucleocapsid-mediated translation initiation.hantavirus nucleocapsid protein (n) can replace the cellular cap-binding complex, eukaryotic initiation factor 4f (eif4f), to mediate translation initiation. although n can augment translation initiation of nonviral mrna, initiation of viral mrna by n is superior. all members of the bunyaviridae family, including the species of the hantavirus genus, express either three or four primary mrnas from their tripartite negative-sense genomes. the 5' ends of the mrnas contain nonviral heterologous olig ...201020573811
roles of human disturbance, precipitation, and a pathogen on the survival and reproductive probabilities of deer mice.climate change, human disturbance, and disease can have large impacts on the dynamics of a species by affecting the likelihood of survival and reproduction of individuals. we investigated the roles of precipitation, off-road vehicle (orv) alteration of habitat, and infection with sin nombre virus on the survival and reproductive probabilities of deer mice (peromyscus maniculatus). we used generalized linear mixed models to estimate the effects of these factors and their interactions by fitting c ...201020392022
hormonal deficiencies during and after puumala hantavirus infection.previous reports have described panhypopituitarism associated with severe cases of hemorrhagic fever with renal syndrome (hfrs), but the prevalence of hormonal deficiencies after nephropathia epidemica (ne), a milder form of hfrs, has not been studied. this study was conducted in order to determine the prevalence of hormonal defects in patients with acute ne and during long-term follow-up. fifty-four patients with serologically confirmed acute ne were examined by serum hormonal measurements duri ...201020397036
tula virus in populations of small terrestrial mammals in a rural landscape.over 5 years (2000-2004), populations of small mammals from a rural landscape in southern moravia (czech republic) were investigated for the presence of tula virus (tulv) antigen using the elisa set hantagnost. in total, 1566 individuals from 10 species were examined. the prevalence in the common vole (microtus arvalis pallas 1778), the main reservoir of tulv, was 10% (n = 871). the prevalence of tulv antigen increases with its population numbers. the highest number of tulv antigen-positive comm ...201020420534
a global perspective on hantavirus ecology, epidemiology, and disease.hantaviruses are enzootic viruses that maintain persistent infections in their rodent hosts without apparent disease symptoms. the spillover of these viruses to humans can lead to one of two serious illnesses, hantavirus pulmonary syndrome and hemorrhagic fever with renal syndrome. in recent years, there has been an improved understanding of the epidemiology, pathogenesis, and natural history of these viruses following an increase in the number of outbreaks in the americas. in this review, curre ...201020375360
hantavirus outbreak associated with laboratory rats in yunnan, china.an outbreak of hemorrhagic fever with renal syndrome occurred among students in a college (college a) in kunming, yunnan province, china in 2003. subsequent investigations revealed the presence of hantavirus antibodies and antigens in laboratory rats at college a and two other institutions. hantavirus antibodies were detected in 15 additional individuals other than the index case in these three locations. epidemiologic data indicated that the human infections were a result of zoonotic transmissi ...201020380897
[construction and identification of a new type of recombinant adenovirus containing s0.7 gene of hantavirus].to construct a adenovirus vector containing the 0.7 kb fragment of s gene of hantavirus, cag promoter, and wpre (mrna-stabilizing post-transcriptional regulatory element from the woodchuck hepatitis virus).201020423645
novel hantavirus in the flat-skulled shrew (sorex roboratus).genetically distinct hantaviruses have been identified recently in multiple species of shrews (order soricomorpha, family soricidae) in eurasia and north america. to corroborate decades-old reports of hantaviral antigens in shrews from russia, archival liver and lung tissues from 4 siberian large-toothed shrews (sorex daphaenodon), 5 eurasian least shrews (sorex minutissimus), 12 flat-skulled shrews (sorex roboratus), and 18 tundra shrews (sorex tundrensis), captured in the sakha republic in nor ...201020426682
hantavirus-like particles generated in cho cells induce specific immune responses in c57bl/6 mice.a safe and effective hantavirus vaccine is highly desirable since hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, hfrs). virus-like particles (vlps) displaying functional viral proteins could provide effective vaccines against a few viruses, but their ability to induce hantavirus-specific immune response has not been adequately investigated. to measure the immunogenicity of hantaan virus-like particles (htn-vlps) vaccine, ...201020433802
cytoplasmic tails of hantavirus glycoproteins interact with the nucleocapsid protein.here we characterize the interaction between the glycoproteins (gn and gc) and the ribonucleoprotein (rnp) of puumala virus (puuv; genus hantavirus, family bunyaviridae). the interaction was initially established with native proteins by co-immunoprecipitating puuv nucleocapsid (n) protein with the glycoprotein complex. mapping of the interaction sites revealed that the n protein has multiple binding sites in the cytoplasmic tail (ct) of gn and is also able to bind to the predicted ct of gc. the ...201020444994
environmental and ecological potential for enzootic cycles of puumala hantavirus in great britain.puumala virus (puuv) is a zoonotic rodent-borne hantavirus in continental europe. its reservoir host, the bank vole (myodes glareolus), is ubiquitous in great britain (gb); however, there has been no reported incidence of virus in either animals or humans. in northwest europe, increases in bank vole numbers, stimulated by increases in production of beech/oak crops (mast), are associated with outbreaks of nephropathia epidemica (ne) in humans. these so-called 'mast years' are determined by sequen ...201019563697
andes virus recognition of human and syrian hamster beta3 integrins is determined by an l33p substitution in the psi domain.andes virus (andv) causes a fatal hantavirus pulmonary syndrome (hps) in humans and syrian hamsters. human alpha(v)beta(3) integrins are receptors for several pathogenic hantaviruses, and the function of alpha(v)beta(3) integrins on endothelial cells suggests a role for alpha(v)beta(3) in hantavirus directed vascular permeability. we determined here that andv infection of human endothelial cells or syrian hamster-derived bhk-21 cells was selectively inhibited by the high-affinity alpha(v)beta(3) ...201019846530
andes virus infections in the rodent reservoir and in humans vary across contrasting landscapes in chile.hantavirus cardiopulmonary syndrome (hcps) is an emerging infectious disease first reported in chile in 1995. andes hantavirus (andv) is responsible for the more than 500 cases of hcps reported in chile. previous work showed that andv is genetically differentiated in chile across contrasting landscapes. to determine whether the reservoir rodent (oligoryzomys longicaudatus) populations are also geographically segregated, we conducted range-wide spatial genetic analyses of o. longicaudatus in chil ...201019632357
a serosurvey reveals bosnia and herzegovina as a europe's hotspot in hantavirus seroprevalence.the extent of hantavirus seroprevalence in the healthy population from bosnia and herzegovina has not yet been investigated; therefore, the aim of this study was to assess the hantavirus seroprevalence in the population from different regions of bosnia and herzegovina and in different risk groups. the serosurvey included 1331 subjects from endemic and non-endemic regions in bosnia and herzegovina. all sera samples were examined using igg elisa, and western blot (bunyavirus igg) tests. hantavirus ...201019951455
puumala virus reference strain for hantavirus serodiagnosis in france. 201019885688
production and characterization of a recombinant single-chain antibody against hantaan virus envelop glycoprotein.hantaan virus (htnv) is the type of hantavirus causing hemorrhagic fever with renal syndrome, for which no specific therapeutics are available so far. cell type-specific internalizing antibodies can be used to deliver therapeutics intracellularly to target cell and thus, have potential application in anti-htnv infection. to achieve intracellular delivery of therapeutics, it is necessary to obtain antibodies that demonstrate sufficient cell type-specific binding, internalizing, and desired cellul ...201020012277
case-control study on puumala virus infection: smoking is a risk factor.puumala hantavirus (puuv) is apparently transmitted to humans by inhalation of aerosolized secretions of carrier rodents (bank voles). the means of transmission and the associated risk factors are poorly defined. an epidemiological study during the peak of an epidemic season in finland was conducted based on 282 acute clinical puuv infections and 204 controls without puuv infection or immunity. the main risk factors adjusted by age, sex and living environment were cigarette smoking [odds ratio ( ...201019781114
genetic detection of dobrava/belgrade virus in a czech patient with haemorrhagic fever with renal syndrome.in the summer of 2008, a 15-year-old boy was hospitalized in a paediatric intensive care unit in the czech republic. laboratory diagnosis of hantavirus infection was established by serological and molecular methods. sequence and phylogenetic analyses showed that the causative strain was dobrava/belgrade virus, which is genetically closer to strains associated with apodemus flavicollis rodents.201019832712
study of andes virus entry and neutralization using a pseudovirion system.andes virus (andv), a member of the hantavirus genus in the family bunyaviridae, causes an acute disease characteristic of new-world hantaviruses called hantavirus pulmonary syndrome (hps). hps is a highly pathogenic disease with a case-fatality rate of 40%. andv is the only hantavirus reported to spread directly from human-to-human. the aim of the present study was to develop a quantitative and high-throughput pseudovirion assay to study andv infection and neutralization in biosafety level 2 fa ...201019903496
reappearance of viral hemorrhagic fever with renal syndrome in northwestern greece.minor outbreaks of viral hemorrhagic fever (vhf) with renal failure have occurred in northwestern greece over past decades. however, during the most recent decade, there has been a paucity of human cases despite the detection of hantavirus in rodents of this area.201019926507
natural host relationships of hantaviruses native to western venezuela.strains of caño delgadito virus (cadv) and maporal virus (mapv) were isolated from 25 (8.9%) of the 280 rodents captured on farms in 1997 in western venezuela. the results of analyses of laboratory and zoographic data indicated that alston's cotton rat (sigmodon alstoni) is the principal host of cadv, horizontal virus transmission is the dominant mode of cadv transmission in alston's cotton rat in nature, a pygmy rice rat (oligoryzomys sp.) is the principal host of mapv, and the natural host rel ...201020055578
[molecular evolution analysis of hantaviruses in zhejiang province].in order to analyze the molecular epidemiology of hantavirus (hv) in zhejiang province, the complete m and s genome sequences of 12 hv strains from different hosts and locations in zhejiang province of china during the period of 1981-2007 were analyzed on genetic evolution by dnastar and mega 4.0 software in this research. phylogenetic analyses revealed that htn and seo strains were co-circulating in zhejiang province, and the difference in sequence similarity and the phylogeny was closely relat ...201021344751
an efficient in vivo method for the isolation of puumala virus in syrian hamsters and the characterization of the isolates from russia.puumala virus (puuv) and other arvicolinae-borne hantaviruses are difficult to cultivate in cell culture. to isolate these hantaviruses efficiently, hantavirus nucleocapsid protein (np)-positive but seronegative wild rodents were selected by np-detection elisa. three of 68 myodes glareolus captured in samara, russia, were np-positive and seronegative. syrian hamsters were inoculated with lung homogenates from np-positive rodents for virus propagation. virus isolation in vitro was carried out by ...201021192975
a pauci-symptomatic case of documented hantavirus (puumala) infection in a patient under anti-tnf treatment.we describe the case of an 18-yr-old male under anti-tnf treatment for crohn's disease for more than 8 months. he developed fever and biological inflammatory syndrome without absolutely no accompanying sign or symptom or paraclinical abnormality despite extensive work-up performed in the context of his immunocompromised state. symptoms disappeared after 10 days and a diagnosis of puumala infection was made retrospectively on a serological basis. the case illustrates that anti-tnf treatment does ...201021195021
[hantavirus infection: two case reports from a province in the eastern blacksea region, turkey.]hantaviruses which are the members of bunyaviridae, differ from other members of this family since they are transmitted to humans by rodents. more than 200.000 cases of hantavirus infections are reported annually worldwide. hantaviruses can lead to two different types of infection in humans, namely, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). hfrs is the most common type of hantavirus infection in europe and asia and the most common virus types are dobra ...201021063999
hantavirus pulmonary syndrome coexistent with dengue.dengue is an arthropod-borne disease caused by viruses of flaviviridae family. it poses a major public health burden in tropical and sub-tropical regions. clinical features of dengue vary from a mild flu-like disease and rash, to a potentially lethal haemorrhagic fever or shock syndrome. hantavirus pulmonary syndrome is a rodent-born disease emerging in the american continent and is caused by viruses of the bunyaviridae family. potential reservoirs of these agents were described in brazilian cen ...201021302604
vegfr2 and src kinase inhibitors suppress andes virus-induced endothelial cell permeability.hantaviruses predominantly infect human endothelial cells and, in the absence of cell lysis, cause two diseases resulting from increased vascular permeability. andes virus (andv) causes a highly lethal acute pulmonary edema termed hantavirus pulmonary syndrome (hps). andv infection enhances the permeability of endothelial cells in response to vascular endothelial growth factor (vegf) by increasing signaling responses directed by the vegfr2-src-ve-cadherin pathway, which directs adherens junction ...201021177802
[morphological findings in fatal cases of hantavirus cardiopulmonary syndrome. report of 7 autopsies].hantavirus cardiopulmonary syndrome (hcps) is an acute infectious disease characterized by sudden cardiorespiratory failure and high mortality, caused by a rna virus of the genus hantavirus, family bunyaviridae, 15% of chilean cases have been detected in the araucania region.201021186503
aromatic and polar residues spanning the candidate fusion peptide of the andes virus gc protein are essential for membrane fusion and infection.hantaviruses infect human cells through cell attachment and subsequent fusion of viral and cellular membranes at low ph. this largely unknown entry process is mediated by the gn and gc glycoproteins, anchored at the viral envelope membrane. performing bioinformatic analysis and peptide-liposome-binding assays we suggested in a former report that gc of andes virus (andv) and other hantaviruses corresponds to the viral fusion protein sharing characteristics with class ii fusion proteins. to gain i ...201021123541
modulation of innate immune responses by hantaviruses.hantavirus infection can cause hantavirus cardiopulmonary syndrome or hemorrhagic fever with renal syndrome depending on the virus species involved. the determinants for the virus species specific virulence in humans are unclear. successful infection is a conditio sine qua non for the virulence of a virus and it is well-known that the innate interferon (ifn) system generally plays a decisive role to prevent establishment of an infection. the importance of the ifn system is underscored by the fac ...201021175415
[epidemiology and surveillance programs on hemorrhagic fever with renal syndrome in mainland china, 2005 - 2008.]objective: to analyze the epidemiologic and surveillance data of hemorrhagic fever with renal syndrome (hfrs) in china, from 2005 to 2008, to describe the epidemiology and trend of hfrs. methods: descriptive epidemiology were studied to analyze the surveillance data from 2005 to 2008, collected from both the internet-based national notifiable disease reporting system and 40 hfrs sentinel sites developed since 2005 in 40 counties around china. results: a total of 56 077 hfrs cases and 692 deaths ...201021163102
a temporal dilution effect: hantavirus infection in deer mice and the intermittent presence of voles in montana.the effect of intermittently occurring, non-reservoir host species on pathogen transmission and prevalence in a reservoir population is poorly understood. we investigated whether voles, microtus spp., which occur intermittently, influenced estimated standing antibody prevalence (esap) to sin nombre hantavirus (snv, bunyaviridae: hantavirus) among deer mice, peromyscus maniculatus, whose populations are persistent. we used 14 years of data from central montana to investigate whether esap among de ...201021170746
the relative abundance of deer mice with antibody to sin nombre virus corresponds to the occurrence of hantavirus pulmonary syndrome in nearby humans.abstract sin nombre virus (snv) is the principal cause of hantavirus pulmonary syndrome (hps) in the united states and deer mice (peromyscus maniculatus) are its principal rodent host, and thus the natural cycle of the virus is related to the occurrence of hps. prevalence of rodent infection appears to be associated with fluctuations in deer mouse populations and, indirectly, with timing and amount of precipitation, a complex of biologic events. given that rodent population abundances fluctuate, ...201020954865
rapid expansion and long-term persistence of elevated nk cell numbers in humans infected with hantavirus.natural killer (nk) cells are known to mount a rapid response to several virus infections. in experimental models of acute viral infection, this response has been characterized by prompt nk cell activation and expansion followed by rapid contraction. in contrast to experimental model systems, much less is known about nk cell responses to acute viral infections in humans. we demonstrate that nk cells can rapidly expand and persist at highly elevated levels for >60 d after human hantavirus infecti ...201021173105
andes virus regulation of cellular micrornas contributes to hantavirus-induced endothelial cell permeability.hantaviruses infect human endothelial cells (ecs) and cause two diseases marked by vascular permeability defects, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). vascular permeability occurs in the absence of ec lysis, suggesting that hantaviruses alter normal ec fluid barrier functions. ecs infected by pathogenic hantaviruses are hyperresponsive to vascular endothelial growth factor (vegf), and this alters the fluid barrier function of ec adherens junctions ...201020844033
[genetic analysis of hantaviruses carried by rattus norvegicus collected from henan and neimenggu provinces, china.]objective: genetic analysis was performed to infer the relationship between hantaviruses carried by rattus norvegicus from henan and neimenggu provinces and the other known hantavirus and the vaccine strain. methods: total rna was extracted from lung tissues with trizol reagent. the complete m and s segment sequences of strains nm133 and q12 were amplified by rt-pcr. the purified dna fragments were directly subjected to sequencing, and then to sequence analysis and phylogenetic analysis. results ...201021162871
temporal variation in individual factors associated with hantavirus infection in bank voles during an epizootic: implications for puumala virus transmission dynamics.abstract puumala virus (puuv), the causal agent of nephropathia epidemica in humans, is one of the many hantaviruses included in the list of emerging pathogens. hantavirus infection is not distributed evenly among puuv reservoir hosts (i.e., bank voles [myodes glareolus]). besides environmental factors and local population features, individual characteristics play an important role in vole puuv infection risk. identifying the relative importance of these individual characteristics can provide cr ...201021142469
identification of a region of hantavirus nucleocapsid protein required for rna chaperone activity.sin nombre hantavirus (snv) is a new world hantavirus and causes hantavirus cardiopulmonary syndrome. the viral nucleocapsid protein (n) is an rna chaperone and has multiple functions important in virus replication. the three negative sense rna segments of hantaviruses form panhandle structures through imperfect hydrogen bonding of the 5' and 3' termini, and the chaperone activity of n can mediate correct panhandle formation. n also functions during transcription and translation initiation and t ...201021378500
hantavirus pulmonary syndrome in the atlantic argentinean patagonia region.the hantavirus causes a hemorrhagic disease and in 1993 was responsible for an outbreak of a pulmonary syndrome in the south of the united states of america. the disease is endemic in the north of argentina where the oran strain is prevalent. a fatal case of the hanta pulmonary syndrome (hps) occurring in a migrant agricultural worker is reported.201021413585
hantaviruses in the americas and their role as emerging pathogens.the continued emergence and re-emergence of pathogens represent an ongoing, sometimes major, threat to populations. hantaviruses (family bunyaviridae) and their associated human diseases were considered to be confined to eurasia, but the occurrence of an outbreak in 1993-94 in the southwestern united states led to a great increase in their study among virologists worldwide. well over 40 hantaviral genotypes have been described, the large majority since 1993, and nearly half of them pathogenic fo ...201021994631
[hantavirus]. 201020942073
cyclic hantavirus epidemics in humans--predicted by rodent host dynamics.wildlife-originated zoonotic diseases are a major contributor to emerging infectious diseases. hantaviruses cause thousands of human disease cases annually worldwide, and understanding and predicting human hantavirus epidemics still poses unsolved challenges. here we studied the three-level relationships between the human disease nephropathia epidemica (ne), its etiological agent puumala hantavirus (puuv) and the rodent host of the virus, the bank vole (myodes glareolus). a large and long-term d ...200921352757
transient hypopituitarism in a patient with nephropathia epidemica.involvement of the pituitary gland is only rarely observed in hantavirus infection. this report describes the case of a patient who had transient hypopituitarism requiring hormonal replacement therapy due to hypophysitis as a result of puumala virus infection. mri studies revealed oedematous swelling of the gland as a morphological correlate. this report provides new evidence that hypopituitarism can be a serious complication in puumala virus infection and highlights the clinical implications of ...200921691389
vaccines for hantaviruses.hantaviruses are assigned to categories a and c on the national institute of allergy and infectious diseases (niaid) priority pathogens list and to category c on the centers for disease control and prevention (cdc) emergency preparedness and response list of boterrorism agents/diseases. these rodent-borne viruses are members of the family bunyaviridae and are transmitted to humans in aerosols of rodent excreta. there are more than 20 recognized hantaviruses, some of which are associated with one ...200919837289
using satellite images of environmental changes to predict infectious disease outbreaks.recent events clearly illustrate a continued vulnerability of large populations to infectious diseases, which is related to our changing human-constructed and natural environments. a single person with multidrug-resistant tuberculosis in 2007 provided a wake-up call to the united states and global public health infrastructure, as the health professionals and the public realized that today's ease of airline travel can potentially expose hundreds of persons to an untreatable disease associated wit ...200919788799
hantavirus pulmonary syndrome in brasilia periphery: a diagnostic challenge.hantavirus pulmonary syndrome (hps) is an emerging rodent-borne disease in the american continent, characterized by acute respiratory distress and a high case-fatality ratio. the present work describes a case of hps, with favorable outcome, whose initial features were mistaken for leptospirosis or other less severe acute infections.200919801809
epidemiological study of hantavirus infection in the samara region of european russia.european russia is a highly endemic area of hemorrhagic fever with renal syndrome (hfrs), a rodent-borne zoonotic disease, caused by hantaviruses. in total, 145 small mammals of four species (myodes glareolus, apodemus flavicollis, a. agrarius, and a. uralensis) were trapped in the samara region of european russia in august 2005 and examined for the presence of hantavirus (hv). anti-hv antibodies were found in six of 68 (8.8%) m. glareolus and in one of 19 (5.3%) a. flavicollis by indirect immun ...200920046023
Refresh
firstprev
12345678910...
nextlast
Displaying items 201 - 300 of 2786