Publications

selective sites of adenovirus (foreign) dna integration into the hamster genome: changes in integration patterns.	we investigated whether, upon the integration of multiple copies of adenovirus type 12 (ad12) dna into an established mammalian (hamster) genome, the pattern of foreign dna insertion would remain stable or change with consecutive passages of cells in culture. by the injection of purified ad12 into newborn hamsters, tumors were induced, cells from these tumors were cultivated, and five independent cell lines, ht5, h201/2, h201/3, h271, and h281, were established. these cell lines carried differen ...	1994	8254728
sequence analysis of bovine adenovirus type 3 early region 3 and fibre protein genes.		1993	8277294
subcloning and restriction enzyme mapping of bovine adenovirus type 2.	bovine adenoviruses (bavs), causing both respiratory and/or enteral diseases in cattle, have been isolated in many countries all over the world. in this study we report on the molecular cloning of the internal ecori fragments spanning 20.6-90.5%, and the internal sali fragments spanning 3.1-65.2% of the bav type 2 (bav2; strain no. 19) genome into the plasmid vector puc19. moreover, the subcloning of the bav2 genome facilitated the construction of a detailed physical restriction endonuclease map ...	1993	8294184
sequence and sequence analysis of e1 and pix regions of the bav3 genome.	bovine adenovirus type 3 (bav3) is a dna virus that causes respiratory and gastrointestinal disorders in cattle. we have sequenced the extreme left end of bav3 genome (0-11.7 map units). partial analysis of the nucleotide sequence revealed 19 potential open reading frames (orfs) that could encode for polypeptides of 50 or more amino acids. four of these orfs show homology to known adenovirus polypeptides. the four bav3 orfs are located in approximately the same area as the ad5 e1a, e1b, and pix ...	1993	8150592
restriction endonuclease analysis and molecular cloning of porcine adenovirus type 3.	the 6618 strain of porcine adenovirus type 3 was cultivated in swine testis cells, and viral dna was extracted from the infected cells by a modified hirt procedure. the dna was digested by each of 13 restriction endonucleases, and the number of cleavage sites which were identified varied from 1 to 17. the size of the porcine adenovirus type 3 genome, estimated from the sizes of the restriction enzyme fragments, was approximately 35 kb. fragments representing the entire genome were cloned. physic ...	1993	8150597
identification of ovine adenovirus types five and six in an epizootic of respiratory tract disease in recently weaned lambs.	eight hundred fourteen polypay-cross lambs, 32 to 35 days old, were housed in an isolation barn under semiconfinement conditions. the lambs were observed twice daily for clinical signs of respiratory tract disease. during the 8-week observation period, an epizootic of respiratory tract disease developed, in which the combined morbidity and mortality was approximately 13%. the overall mortality was 4.1%, of which 57.6% was attributable to pneumonia. pasteurella haemolytica was isolated from 11 (5 ...	1987	3038805
[comparative study of the hexons in adenoviruses of the genus mastadenovirus].		1986	2478865
inoculation of lambs with ovine adenovirus 5 (mastadenovirus ovi 5) strain rts-42.	twelve 1-week-old colostrum-deprived lambs inoculated with the rts-42 strain of mastadenovirus ovi 5 were killed and necropsied (2 lambs/day) on postinoculation days (pid) 2, 4, 6, 8, 12, and 21. four noninoculated lambs were killed and necropsied (2/day) on pid 6 and 12. virus was isolated from nasal secretions and feces on pid 1 to pid 6, from tracheal fluids and lung tissue of lambs necropsied on pid 2, 4, and 6, and from lung tissue from 1 lamb necropsied on pid 8. virus was not recovered fr ...	1986	3008604
pulmonary lesions in lambs experimentally infected with ovine adenovirus 5 strain rts-42.	twelve lambs were inoculated transtracheally and intranasally with mastadenovirus ovi 5 strain rts-42 and killed sequentially. pulmonary lesions were studied by light and electron microscopy. four lambs served as sham inoculated controls. pulmonary lesions consisted of multifocal areas of bronchiolitis and alveolitis associated with necrosis and sloughing of isolated type i and type ii alveolar epithelial cells and nonciliated bronchiolar epithelial cells. this was followed rapidly by hyperplasi ...	1986	3022454
seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs.	a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ...	1985	3002217
should the criteria for species distinction in adenoviruses be reconsidered? evidence from canine adenoviruses 1 and 2.	four strains of canine adenovirus type 1 (cav-1) and two strains of canine adenovirus type 2 (cav-2) were examined by restriction enzyme analysis. in all cases, cav-1 could be readily differentiated from cav-2. additionally, monoclonal antibodies were prepared against the mirandola and manhattan strains of cav-1 and cav-2, respectively. 18 of 36 monoclonal antibodies from the cav-1 fusion and 77 of 160 monoclonal antibodies from the cav-2 fusion were type-specific by an indirect fluorescent-anti ...	1985	3884549
adenovirus 38: a new human adenovirus species of subgenus d.	from the stool of a dutch girl, a new virus (lj) was isolated that by morphological, biological, and immunological criteria was a human adenovirus. because of its hemagglutinating activity for rat and human red blood cells and its dna restriction enzyme pattern, the virus was classified as a member of subgenus d. it was distinct in neutralization tests from all other human adenoviruses. the hemagglutinin was antigenically similar to that of adenoviruses 13 and 39, but the dna differed from that ...	1984	6094388
isolation and partial characterization of a human adenovirus type 4 temperature-sensitive mutant (mastadenovirus h 4 tsl).	a random selection procedure was used to isolate a temperature-sensitive (ts) mutant of human adenovirus type 4 (mastadenovirus h 4 tsl) from nitrous acid mutagenized virus stock. the mutant displayed restricted growth at the nonpermissive temperature of 39 degrees c. analysis of the mutant grown at 39 degrees c, by two-dimensional immunoelectrophoretic analysis, showed the mutant to be defective in the expression of the penton base and fibre structural components. the mutant was, however, capab ...	1984	6713306
adenovirus pneumonia in guineapigs: an experimental reproduction of the disease.	the recently described virus-induced pneumonia in guineapigs (naumann et al., 1981) was experimentally reproducible in newborn animals, though not in preadult animals. baby hamsters and newborn rats were also not susceptible to infection. 10 of 11 infected newborn guineapigs developed pathological changes identical with those found in spontaneous cases. the incubation period was from 5 to 10 days. the agent could not be cultivated in vitro, and therefore no applicable serological tests could be ...	1984	10628789
candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool.	about 200 antigenically related adenoviruses were isolated from cases of infantile diarrhoea in the netherlands and north-west germany. the viruses were fastidious and failed to replicate serially in human diploid fibroblasts and in primary human embryonic kidney cells. a number of strains were established in hela, hep-2, graham (293), cynomolgus monkey kidney, and chang conjunctival cells. the viruses were mammalian adenoviruses by the usual criteria. no relationship to the 39 known human adeno ...	1983	6306161
characteristics of mastadenovirus h 8, the causative agent of epidemic keratoconjunctivitis.	adenovirus 8 (ad 8, strain 1127) has the general structural features of an adenovirus; it produces the genus-specific hexon antigen. the fiber length of 12 nm corresponds to that of other subgenus d adenoviruses. in hemagglutination-inhibition, ad 8 is related to ad9 and ad 15/h9. the slow multiplication, compared to most other subgenus d viruses, was shown not to be caused by an extended growth cycle, but appears to be due to an inefficient virus release into the medium. after restriction analy ...	1983	6312928
new human adenovirus associated with respiratory illness: candidate adenovirus type 39.	a new human adenovirus was isolated from a stool specimen from a 2-year-old el salvadorian male hospitalized with severe respiratory illness. the virus, strain d335, is a typical adenovirus by routine classification tests and bears little resemblance to the other 38 adenovirus serotypes by standard hemagglutination inhibition and serum neutralization tests. the only significant cross-reaction observed was between adenovirus types 39 and 13, bilaterally, by the hemagglutination inhibition test. t ...	1982	6179962
immunological relationship among human adenoviruses of subgenus d.	antigenic relationships between adenoviruses of subgenus d were determined by neutralization tests in hela cell cultures by cpe inhibition. for cross-testing, several antisera of the same species were tested against the prototype viruses. 39 wild strains belonging to 12 different virus species were also studied. marked variation in the degree of cross-neutralization between individual sera of the same species was often observed. however, virus strains within a species mostly showed identical ser ...	1982	6180704
adenoviridae.	the elevation of the genus adenovirus to a family named adenoviridae is described. this family at the present stage contains two genera, mammalian adenoviruses--mastadenovirus--and avian adenoviruses--aviadenovirus. the two genera are separated primarily on the basis of difference in immunological properties of the virions.	1976	1002415